Publications

demonstration of non-degraded aleutian disease virus (adv) proteins in lung tissue from experimentally infected mink kits. brief report.	isolates of adv replicate to rather high quantities in lungs from neonatally infected mink kits. the virus was analysed for polypeptide composition, and for the first time high molecular weight polypeptides have been observed in in vivo produced advs. these polypeptides are analogous to those of in vitro produced advs. the molecular weights of the structural polypeptides of the low virulence pullman adv and the highly virulent dk and utah i isolates of adv were found to be 88kd and 78kd and in v ...	1986	3002299
central nervous system lesions in suckling mice and rats inoculated intranasally with sialodacryoadenitis virus.	suckling cd-1 and cfw mice and wi wistar rats were inoculated intranasally with sialodacryoadenitis virus. in animals inoculated during the first week of life, there was an acute necrotizing encephalitis with malacia and minimal inflammatory cell response. in mice and rats which survived for up to 12 days post inoculation, loss of brain substance, gitter cells and occasionally mineralized debris were seen. viral antigen was readily demonstrated in neurons and nasal epithelium in trypsin-treated, ...	1986	3004003
tn4351 transposes in bacteroides spp. and mediates the integration of plasmid r751 into the bacteroides chromosome.	the gene for resistance to erythromycin and clindamycin, which is carried on the conjugative bacteroides plasmid, pbf4, has been shown previously to be part of an element (tn4351) that transposes in escherichia coli. we have now introduced tn4351 into bacteroides uniformis 0061 on the following two suicide vectors: (i) the broad-host-range incp plasmid r751 (r751::tn4351) and (ii) pss-2, a chimeric plasmid which contains 33 kilobases of pbf4 (including tn4351) cloned into the incq plasmid rsf101 ...	1986	3005243
[morphological changes caused by adrenalectomy in rabbits].		1986	3019628
[morphological changes caused by adrenalectomy in rabbits].		1986	3019628
experimental models of type-i diabetes.		1986	3110756
the effect of insulin and catecholamines on the activities of 3-hydroxy-3-methyl glutaryl coenzyme a reductase and acyl-coenzyme a: cholesterol-o-acyltransferase in isolated rat hepatocytes.	this study was concerned with the effect of insulin and catecholamines on the rate limiting enzymes of cholesterol metabolism in rat hepatocytes. insulin was found to increase the activity of 3-hydroxy-3-methyl glutaryl coenzyme a reductase and to have no effect on the activity of acyl-coenzyme a: cholesterol-o-acyltransferase. noradrenaline and isoprenaline increased the activities of both 3-hydroxy-3-methyl glutaryl coenzyme a reductase and acyl-coenzyme a: cholesterol-o-acyltransferase. the e ...	1986	3516768
low-grade lymphomas in young homosexual men without lav/htlv iii infection.		1986	2877052
bvd virus infection and embryo transfer.		1986	3716157
18q+, the progeny of a balanced translocation t(1;18)mat: case report with necropsy findings.	a female infant with additional genetic material on the long arm of chromosome 18 is described. cytogenetic studies of the infant and her mother showed that the altered region resulted from an unbalanced translocation of part of the long arm of chromosome 1. this chromosomal abnormality has not been reported previously, according to a recent registry of abnormal chromosome patterns. the patient had hydrops fetalis and multiple congenital abnormalities, involving the cardiovascular, respiratory, ...	1986	3723558
pressure evaluation of the antireflux ability of the rat ureterovesical junction.	the bladder pressure necessary to cause vesicoureteral reflux (vur) was measured in 16 female rats. under general anesthesia, the ureters were exposed via an abdominal incision and a pressure catheter was placed near the ureterovesical junction. values of bladder distension and bladder pressure increase to cause vur were obtained by injecting isotonic saline in one ureter until vur in the opposite ureter was detected as a sudden pressure increase. after 5 min the same procedure was done on the c ...	1986	3750580
the relationship between motor activity and transmural potential difference in the guinea pig intestine in vitro: is there a neural link?	the aim of the experiments was to examine, in vitro, the role of the enteric nervous system in the relationship between motor activity and transmural potential difference (pd) in the guinea pig jejunum and colon using the nerve blocking agents tetrodotoxin (ttx) and aconitine. histological data showed that perfusion of the intestinal segments with gassed hepes solution was essential for the maintenance of transmural pd. disruption of the mucosa was associated with a loss of spontaneous fluctuati ...	1986	3768803
[kinetics of the iha antibody response before and after pyquiton treatment in rats infected with paragonimus westermani].		1986	3791589
detoxication of cyanide in the chicken by conversion to thiocyanate, as influenced by the availability of transferable sulphur.	the urinary excretion of thiocyanate by hens after dosage with cyanide (30 mumol) has been studied in a series of acute experiments involving 6 hr urine collection periods. more than half of the dose could be recovered as thiocyanate when cyanide was given by intravenous infusion and the rate of excretion closely paralleled plasma thiocyanate concentration. little cyanide was excreted directly. the excretion of thiosulphate fell by an amount that suggested that availability of sulphane sulphur m ...	1987	3829625
hydrolysis of dietary flavonoid glycosides by strains of intestinal bacteroides from humans.	rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. the enzymes required for these hydrolyses, namely alpha-rhamnosidase and beta-galactosidase, were produced by some strains of bacteroides distasonis; other strains, however, synthesized beta-glucosidase. the last-named enzyme was also elaborated by bacteroides uniformis and bacteroides ovatus. all the enzymes were produced constitutively. a cell-free extract of b. dis ...	1987	3435494
[cardiac defibrillation. experimental and theoretical aspects].		1987	3577372
identification in rat liver and serum of water-soluble class i mhc molecules possibly homologous to the murine q10 gene product.	we have identified large quantities of a water-soluble, non-rt1.a class i mhc molecule in the serum of the da rat strain, with a similar molecule being found in aqueous extracts of da liver. the non-rt1.a class i molecules have heavy chains of 41 kd, which is smaller than rt1.a class i molecules isolated from liver membranes (45 kd) but larger than water-soluble rt1.a class i molecules previously identified in serum and aqueous extracts of liver and kidney (40 kd). nh3-terminal amino acid sequen ...	1987	3585249
serological evaluation of the outer membrane protein complexes of five saccharolytic intestinal bacteroides species.	the purpose of this investigation was to develop a serological procedure for rapid identification of the following five bacteroides species: bacteroides distasonis, bacteroides fragilis, bacteroides ovatus, bacteroides uniformis, and bacteroides vulgatus. the outer membrane fractions were assayed using sds-polyacrylamide slab gel electrophoretic techniques. the species-specific protein band from each species as well as the group-specific protein were purified and used to develop an indirect enzy ...	1987	3665895
the binding of decomposition products of udp-galactose to the microsomes and polyribosomes isolated from rat liver.	udp-d-[u-14c]galactose is decomposed to [u-14c]galactose-1-phosphate and [u-14c]galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. udp-d-[u-14c]galactose, at neutral ph, is also chemically degraded to the [u-14c]galactose-1,2-cyclic phosphate. the 1,2-cyclic phosphate derivative of galactose also exists in the commercial udp-d-[u-14c]galactose. it is a very important finding that products of the udp-d-[u ...	1987	3675917
evaluation of the syphilis bio-enzabead assay for detection of treponemal antibody.	an enzyme-linked immunosorbent assay for treponema pallidum antibody (syphilis bio-enzabead kit; organon teknika corp., formerly litton bionetics, kensington, md.) was compared with the standard fluorescent treponemal antibody absorption test for syphilis (indirect fluorescent-antibody confirmatory test; zeus scientific inc., raritan, n.j.). six hundred specimens tested in the rapid plasma reagin card test (hynson, westcott and dunning, inc., baltimore, md.) and microhemagglutination assay for a ...	1987	3294884
a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis.	six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo.	1987	3329531
the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.	the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ...	1987	3117781
[feed-dependent changes in the rumen mucosa of high-producing cows from the dry period to eight weeks post partum].		1987	3122467
u3 sequences from htlv-i and -ii ltrs confer px protein response to a murine leukemia virus ltr.	human t-cell leukemia virus (htlv) types i and ii are unusual among replication-competent retroviruses in that they contain a fourth gene (chi) necessary for replication. the chi gene product, p chi, transcriptionally transactivates the viral long repeat (ltr), and is thus a positive regulator. to investigate p chi transactivation, sequences from the u3 regions of the ltrs of htlv-i and -ii were inserted into the moloney murine leukemia virus (m-mulv) ltr by recombinant dna techniques. transient ...	1987	3027896
facilitated transfer of incp beta r751 derivatives from the chromosome of bacteroides uniformis to escherichia coli recipients by a conjugative bacteroides tetracycline resistance element.	the broad-host-range incp beta plasmid r751 can mobilize itself from escherichia coli to bacteroides spp, but it is not maintained in bacteroides spp. if r751 carries the bacteroides transposon tn4351, it can be integrated into the bacteroides chromosome. previously we showed that r751, integrated in the chromosome of bacteroides uniformis, cannot mobilize itself out of b. uniformis into e. coli or isogenic b. uniformis strains. in this report, we showed that if the bacteroides conjugative tetra ...	1987	3036772
structure and expression of the hepatitis b virus genome.		1987	2948897
[interferon production in vitro].	interferon production in vitro was induced by poly i: c and pha in leukocytes obtained from patients with cancer, benign diseases and control subjects. the interferon response per lymphocyte was relatively constant in all groups. however, interferon production was slightly inhibited in patients receiving cancer chemotherapy. the peak response of interferon occurred at 48 hours after the initiation of the combined culture in each specimen. from our observations, it was suggested that interferon p ...	1987	2952067
[the growth curve. its interest in orthodontics. utilization of the radius of the hand].		1987	212365
insulin action inhibits insulin-like growth factor-ii (igf-ii) receptor phosphorylation in h-35 hepatoma cells. igf-ii receptors isolated from insulin-treated cells exhibit enhanced in vitro phosphorylation by casein kinase ii.	insulin caused a rapid, dose-dependent increase in the binding of 125i-insulin-like growth factor-ii (igf-ii) to the surface of cultured h-35 hepatoma cells. the [32p]phosphate content of the igf-ii receptors, immunoprecipitated from extracts of h-35 cell monolayers previously incubated with [32p]phosphate for 24 h, was decreased after brief exposure of the cells to insulin. analysis of tryptic digests of labeled igf-ii receptors by bidimensional peptide mapping revealed that the decrease in the ...	1988	2963823
tetracycline-dependent appearance of plasmidlike forms in bacteroides uniformis 0061 mediated by conjugal bacteroides tetracycline resistance elements.	some human colonic bacteroides strains carry conjugal tetracycline resistance (tcr) elements, which are thought to be chromosomal. we have found that some of these tcr elements can mediate the appearance of plasmidlike forms in bacteroides uniformis 0061. when b. uniformis 0061, containing a conjugal tcr element designated tcr erl, was grown in medium containing tetracycline (1 microgram/ml), two circular dna forms were found in the alkaline plasmid preparations: nbu1 (10.3 +/- 0.5 kilobases) an ...	1988	2832373
high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen.	sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ...	1988	2832429
[human chorionic prealbumin: its identification, physicochemical properties and detection in the blood serum in trophoblastic disease].	human chorionic prealbumin (hcpa) was immunochemically identified and isolated in pure form from immature placenta, particularly, from chorion. comparative immunodiffusion analysis shows that hcpa differs from the known placenta-specific proteins. hcpa is a glycoprotein with mw about 100 kd determined by gel-filtration and 45 kd by sds polyacrylamide gel electrophoresis. by immunodiffusion hcpa was not found in fetal and adult tissues and in biological fluids. hcpa was discovered in patients wit ...	1988	2848598
antiviral chemoprophylaxis for cytomegalovirus infection.		1988	2849602
intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection.	seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ...	1988	3134244
evaluation of the quality of bacteriologic methods used to diagnose tuberculosis in argentina.		1988	3147118
subendocardial and subepicardial wall thickening during ischemia in exercising dogs.	to determine whether ischemia in the exercising dog is associated with preservation of subepicardial thickening relative to subendocardial thickening, 10 dogs were chronically instrumented with circumflex artery flow probes, hydraulic occluders, and pairs of ultrasonic microcrystals for determination of wall thickness in the circumflex artery distribution. one pair of crystals spanned the entire ventricular wall (transmural), and the other spanned the outer half of the ventricular wall. inner wa ...	1988	3180383
neuropathological definition of alzheimer disease: multivariate analyses in the morphometric distinction between alzheimer dementia and normal aging.	although establishing that a patient is suffering from dementia of the alzheimer type initially reflects a clinician's opinion, neuropathological study is for the present the most definitive examination to confirm the clinical diagnosis of alzheimer disease. we review several comprehensive publications attempting on a quantitative basis to differentiate the changes occurring with normal aging of the human brain from those indicative of alzheimer disease. new morphometric data on 5 histopathologi ...	1988	3207478
individual free fatty acids and lactate uptake in the human heart during severe sepsis.	coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ...	1988	3232957
mobilization of bacteroides plasmids by bacteroides conjugal elements.	a 4.2-kilobase cryptic bacteroides plasmid, pb8-51, is found in several colonic bacteroides species. to determine whether pb8-51 is mobilized by any of the known bacteroides conjugal elements, we constructed an escherichia coli-bacteroides shuttle vector, pval-1, which contains pb8-51. we constructed bacteroides uniformis 0061 derivatives which carry pval-1 and various bacteroides conjugal elements. the bacteroides conjugal elements tested were six conjugal tetracycline resistance (tcr) elements ...	1988	3343220
evidence of estrogen receptors in normal human osteoblast-like cells.	in seven strains of cultured normal human osteoblast-like cells, a mean of 1615 molecules of tritium-labeled 17 beta-estradiol per cell nucleus could be bound to specific nuclear sites. the nuclear binding of the labeled steroid was temperature-dependent, steroid-specific, saturable, and cell type-specific. these are characteristics of biologically active estrogen receptors. pretreatment with 10 nanomolar estradiol in vitro increased the specific nuclear binding of progesterone in four of six ce ...	1988	3388021
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
effects of serum testosterone level with buserelin on the activities of drug and testosterone hydroxylase and on the content of a male-specific form of cytochrome p-450 in male rats.	the effects of administration of buserelin, a synthetic agonist of lutenizing hormone-releasing hormone (lh-rh), on the content of p-450-male, a male specific form of cytochrome p-450, and the activities of drug and testosterone hydroxylases were examined in liver microsomes of male rats. administration of buserelin resulted in a decrease in the serum level of testosterone in a dose-dependent manner. similar decreases were seen in the activities of aminopyrine n-demethylase, 7-propoxycoumarin o- ...	1989	2492807
morphological response of mycoplasma to fleroxacin.	quinolones exhibit partially reduced activity in vitro against genital mycoplasma. therefore, the morphological response to fleroxacin, a difluorinated quinolone, by mycoplasma hominis was studied. even at subinhibitory concentrations the ultrastructural investigations revealed distinct alterations such as cytolysis, vacuole and cell "ghost" formation. the typical dumbbell-shaped cells observed with monofluorinated compounds were not found, which may indicate a qualitatively different mode of ac ...	1989	2515157
evidence that sindbis virus nsp2 is an autoprotease which processes the virus nonstructural polyprotein.	the four nonstructural proteins (nsp1-4) of sindbis virus, a member of the togaviridae family, are initially expressed from the 5' segment of the single-stranded genomic (+)rna as a polyprotein which is subsequently proteolytically processed. in attempts to identify the protease acting on this nonstructural polyprotein, we established a coupled in polyprotein, we established a coupled in vitro transcription-translation system which was able to faithfully process the major polyprotein when an mrn ...	1989	2525839
specificity spillover at the hormone receptor--exploring its role in human disease.		1989	2537464
electroporation induced transformation of bacteroides ruminicola and bacteroides uniformis by plasmid dna.	frequencies of greater than 10(5) transformants per microgram dna were achieved in bacteroides ruminicola f101 by electroporation of cells under anaerobic conditions, using the 19.5 kbp tetracycline resistance plasmid prri4. similar procedures gave frequences of 10(6) erythromycin resistant transformants per microgram dna with the shuttle plasmid pdp1 (19 kbp) in bacteroides uniformis. transformation of b. uniformis occurred at a far lower frequency (10(3) micrograms) when pdp1 dna was derived f ...	1989	2599352
omental autoimplantation of splenic tissue and intravenous pneumococcal challenge. a comparative study in rats.	splenectomized subjects show a higher incidence of myocardial ischemia and of overwhelming post-splenectomy infection (opsi). it is doubtful that implanted splenic tissue guarantees an adequate protection from opsi. the histological characteristics and the capacity of protection from opsi of two models of omental autoimplantation of splenic tissue in rats were examined. the implanted splenic tissue offers a significant protection from pneumococcal sepsis, even though there is no relation between ...	1989	2628385
cloning and characterization of a bacteroides conjugal tetracycline-erythromycin resistance element by using a shuttle cosmid vector.	the bacteroides conjugal tetracycline resistance (tcr) elements appear not to be plasmids. in many cases, resistance to erythromycin (emr) is cotransferred with tcr. using a newly constructed shuttle cosmid, pnjr1, we cloned 44 to 50 kilobase pairs of a conjugal tcr emr element on overlapping cosmid clones. cosmid libraries were made in escherichia coli with dna from the original clinical bacteroides thetaiotaomicron dot strain containing tcr emr-dot or from a bacteroides uniformis tcr emr-dot t ...	1989	2646276
monoclonal antibody-defined human endothelial antigens as vascular markers.	a review is given of human endothelial antigens recognized by monoclonal antibodies and used as vascular markers. these antigens can be classified tentatively into two categories that partly overlap: 1) differentiation markers and 2) antigens involved in specific cellular functions. monoclonal antibodies recognizing endothelial differentiation markers reacting with all types of human endothelium can be regarded as constitutive endothelial markers. other differentiation markers have a restricted ...	1989	2666520
pathogenicity of the bacteroides fragilis group.	the bacteroides fragilis group is one of the most important pathogens in polymicrobial infections. the distribution of the different members of the b. fragilis group in clinical infections varies. bacteroides fragilis accounts for 63 percent of all the group isolates, bacteroides thetaiotaomicron for 14 percent, bacteroides vulgatus and bacteroides ovatus for seven percent each, bacteroides distasonis for six percent and bacteroides uniformis for two percent. all members of the group induced bac ...	1989	2679351
minitek urea disk test, a sensitive and cost-effective method to screen for campylobacter pylori in gastric biopsies.	one hundred fifty-five biopsy specimens from the gastric mucosa of 81 patients undergoing routine endoscopy procedures were tested for the presence of campylobacter pylori by three methods: gram stain, culture, and modified minitek, a rapid urea disk test (bbl microbiology systems, cockeysville, md.). twenty-nine patients were infected with c. pylori. sensitivities and specificities of detection were 100 and 94% with the minitek test and 93 and 100% with gram stain, respectively. rapid testing b ...	1989	2687317
design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum).	the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ...	1989	2708344
protein kinase c is activated in glomeruli from streptozotocin diabetic rats. possible mediation by glucose.	glomerular inositol content and the turnover of polyphosphoinositides was reduced by 58% in 1-2 wk streptozotocin diabetic rats. addition of inositol to the incubation medium increased polyphosphoinositide turnover in glomeruli from diabetic rats to control values. despite the reduction in inositol content and polyphosphoinositide turnover, protein kinase c was activated in glomeruli from diabetic rats, as assessed by an increase in the percentage of enzyme activity associated with the particula ...	1989	2708528
use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine.	4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%.	1989	2713417
antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.	the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ...	1989	2769234
adverse effects of the use of unusual phenethylamine compounds sold as illicit amphetamine.	a 40-year-old man, who had regularly taken illicit amphetamine by intra-nasal inhalation for several years without ill effects, was admitted to hospital with signs of massive adrenergic overstimulation shortly after inhaling material which he had purchased in the belief that it was amphetamine. the administration of the beta-blocker practolol produced a paradoxical increase in blood pressure. after his discharge from hospital he suffered disabling feelings of anxiety for several weeks. analysis ...	1989	2770471
direct measurement of basolateral membrane potentials from cells of the macula densa.	at the present time, little is known concerning the electrophysiology of the cells of the macula densa and whether or not these cells are electrically responsive to alterations in luminal fluid composition. to investigate this issue, cortical thick ascending limbs (ctal) containing macula densa and attached glomeruli were dissected from rabbit kidney and the ctal perfused in vitro. basolateral membrane potential (vbl) was measured with microelectrodes in macula densa cells and, for comparison, i ...	1989	2782426
the role of calcium in the development of diastolic force during hypoxia in rat myocardium.		1989	2798517
spontaneous tumors in f-344/jcl rats.	the age-related incidence of spontaneously occurring neoplastic and non-neoplastic lesions in untreated f-344/jcl rats, used as controls in carcinogenicity testing, were studied from the histological examination of tissues from 469 males and 354 females. the incidence of spontaneous tumors was 83.2% in the males and 71.2% in the females. the most common neoplasms were leukemia (males: 24.3%, females: 24.0%), pituitary adenoma (males: 16.0%, females: 45.2%), pheochromocytoma (males: 14.7%, female ...	1989	2810400
the role of alpha- and beta-adrenoceptor subtypes in mediating the effects of catecholamines on fasting glucose and insulin concentrations in the rat.	1. the role of alpha- and beta-adrenoceptor subtypes in the regulation of plasma glucose and immunoreactive insulin (iri) levels has been investigated in normal conscious fasted rats by employing selective agonists and antagonists. 2. adrenaline (0.2 mg kg-1)-induced hyperglycaemia was abolished by the selective alpha 2-adrenoceptor antagonist idazoxan (1.0 mg kg-1), unaltered by non-selective beta-adrenoceptor blockade (propranolol, 1.0 mg kg-1) and potentiated by the selective alpha 1-adrenoce ...	1990	1976400
plasmid transformation of bacteroides spp. by electroporation.	transformation of bacteroides spp. with a variety of plasmid dnas was accomplished using electroporation. the standard transformation assay system used to deduce the optimal electroporation parameters employed a 50-to 100-fold concentrated cell suspension of mid-logarithmic phase bacteroides fragilis strain 638 and the 5.4-kb clindamycin resistance (ccr) vector, pbi191. a variety of electroporation buffers were used successfully in transformation experiments but of these, 1 mm mgcl2 in 10% glyce ...	1990	2096397
role of bacteroides uniformis in susceptibility of ta:cf# 1 mice to infection by treponema hyodysenteriae.	among the various strains and colonies of mice tested specific-pathogen-free (spf) ta:cf#1 mice exhibited the highest susceptibility to treponema hyodysenteriae infection. staphylococcus epidermidis 1047, streptococcus faecalis 2039, lactobacillus murinus 3010, escherichia coli 1049, and bacteroides thetaiotaomicron 3008 were administered to the ta:cf#1 mice. b. uniformis ik, which appeared to colonize in the breeding facility under spf conditions, was isolated from the cecal content of a ta:cf# ...	1990	2148089
a cryptic 65-kilobase-pair transposonlike element isolated from bacteroides uniformis has homology with bacteroides conjugal tetracycline resistance elements.	a 65-kilobase-pair element, xbu4422, which has some transposonlike characteristics but carries no known antibiotic resistance genes, has been isolated from bacteroides uniformis 0061. xbu4422 was trapped on bacteroides-escherichia coli shuttle vectors during experiments in which one of the conjugal bacteroides tetracycline resistance (tcr) elements was being used to mobilize the shuttle vectors to bacteroides recipients. results of southern hybridization experiments showed that xbu4422 is normal ...	1990	2156799
the region of a bacteroides conjugal chromosomal tetracycline resistance element which is responsible for production of plasmidlike forms from unlinked chromosomal dna might also be involved in transfer of the element.	large (greater than 50 kilobases) conjugal chromosomal tetracycline resistance (tcr) elements have been found in many human colonic bacteroides strains. recently, n. b. shoemaker and a. a. salyers (j. bacteriol, 170:1651-1657, 1988) reported that some of these tcr elements appeared to mediate production of plasmidlike forms, nbu1 and nbu2, from an unlinked region of the chromosome of bacteroides uniformis 0061. production of the plasmidlike forms and the transfer frequency of the tcr elements we ...	1990	2165473
[new antitubercular drugs].		1990	2168041
non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report.		1990	2223690
heterologous expression of the bacteroides ruminicola xylanase gene in bacteroides fragilis and bacteroides uniformis.	a cloned xylanase gene from the ruminal bacterium bacteroides ruminicola 23 was transferred by conjugation into the colonic species bacteroides fragilis and bacteroides uniformis by using the escherichia coli-bacteroides shuttle vector pval-1. the cloned gene was expressed in both species, and xylanase specific activity in crude extracts was found to be at least 1400-fold greater than that found in the b. ruminicola strain. analysis of crude extract proteins from the recombinant b. fragilis by s ...	1990	2323546
conjugal transfer of a shuttle vector from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4.	prevotella ruminicola (formerly bacteroides ruminicola) is an anaerobic, gram-negative, polysaccharide-degrading bacterium which is found in the rumina of cattle. since p. ruminicola is thought to make an important contribution to digestion of plant material in rumina, the ability to alter this strain genetically might help improve the efficiency of rumen fermentation. however, previously there has been no way to introduce foreign dna into p. ruminicola strains. in this study we transferred a sh ...	1991	1768083
the anti-tachycardic mechanism of a direct-acting vasodilator, budralazine, in rats.	the present study was undertaken to elucidate the anti-tachycardic effect of a direct-acting vasodilator, budralazine, using an electrophysiological technique. normotensive male wistar rats were used. rats were anesthetized intraperitoneally with urethane and alpha-chloralose. intravenous administration of budralazine (0.5-5.0 mg/kg) produced a dose-dependent reduction of mean arterial pressure in anesthetized rats. at doses of 0.5 and 1.0 mg/kg, budralazine induced bradycardia accompanied with ...	1991	1773498
effect of testosterone on the pressor response to common carotid occlusion in gonadectomized conscious male rats with lesion of the median eminence.	the influence of testosterone on the development of the pressor response to common carotid occlusion was investigated in control and median eminence-lesioned male rats. in control rats (n = 9), gonadectomy performed 21 days before the experiments reduced by 22% (from 51 +/- 2 to 40 +/- 2 mmhg) and treatment with testosterone (300 micrograms for 4 days before the measurements) increased the initial peak pressor response (from 51 +/- 2 to 57 +/- 2 mmhg) which depends on carotid innervation. the ma ...	1991	1797293
effect of testosterone on the pressor response to common carotid occlusion in gonadectomized conscious male rats with lesion of the median eminence.	the influence of testosterone on the development of the pressor response to common carotid occlusion was investigated in control and median eminence-lesioned male rats. in control rats (n = 9), gonadectomy performed 21 days before the experiments reduced by 22% (from 51 +/- 2 to 40 +/- 2 mmhg) and treatment with testosterone (300 micrograms for 4 days before the measurements) increased the initial peak pressor response (from 51 +/- 2 to 57 +/- 2 mmhg) which depends on carotid innervation. the ma ...	1991	1797293
evaluation of the rapid id 32a system for the identification of bacteroides fragilis and related organisms.	this study evaluated the ability of a rapid identification system for anaerobic bacteria, atb 32a, now renamed rapid id 32a (api-biomérieux uk ltd., basingstoke), to identify accurately 74 strains of the 'b. fragilis group'. atb 32a identified correctly 78.4% of strains to species level, without supplemental tests. the percentage of strains identified to species level rose to 94.6% when a supplementary test (advised by biomérieux) for catalase production was used to differentiate between bactero ...	1991	1960112
german multicentre study on the in vitro susceptibility of bacteroides species. the german bacteroides study group.	in 1990 the first german multicentre study on the in vitro susceptibility of bacteroides species was completed. employing a commercially prepared microbroth dilution assay, nine participating institutions evaluated approximately 100 consecutive isolates of bacteroides species from relevant clinical specimens. a total of 911 strains (449 bacteroides fragilis, 201 bacteroides thetaiotaomicron, 79 bacteroides ovatus, 78 bacteroides vulgatus, 77 bacteroides distasonis, 25 bacteroides uniformis, 2 ot ...	1992	1295762
effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum.	the effect of three beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam used in clinical practice were compared for their activity against purified beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. the enzymes from b. uniformis and c. butyricum were produced in fermenters under controlled growth conditions and the enzyme from f. nucleatum was produced in batch cultures. purification of the beta-lactamases was achieved by anion-exchang ...	1992	1331018
effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria.	three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and fplc. the inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. various concentrations of the inhibitors were prei ...	1992	1338315
recovery of bacteroides fragilis group from clinical specimens following antimicrobial therapy.	over a period of 14 years (1973-1987), 3165 specimens submitted to the microbiology laboratory demonstrated the recovery of anaerobic bacteria. a total of 988 bacteroides fragilis group isolates were recovered (0.3 isolates per specimen). bacteroides fragilis accounted for 62% of the total of all b. fragilis group isolates, bacteroides thetaiotaomicron for 15%, bacteroides vulgatus for 8%, bacteroides ovatus for 7%, bacteroides distasonis for 6%, and bacteroides uniformis for 2%. of the 988 b. f ...	1992	1393825
antibiotic sensitivity of the bacteroides fragilis group in europe. european study group.	rates of resistance to 12 antibiotics were determined for 1,289 isolates of bacteroides fragilis group submitted in 1988-1989 by 22 laboratories in 15 european countries. there was no resistance to metronidazole (breakpoint 8 mg/l) and only one isolate was resistant to chloramphenicol (breakpoint 8 mg/l). resistance was uncommon for imipenem (0.3% at greater than 4 mg/l), amoxicillin/clavulanate (1% at greater than 8 mg/l), cefoxitin (3% at greater than 32 mg/l), mezlocillin (6% at greater than ...	1992	1396748
thermally-induced delayed fluorescence of photosystem i and ii chlorophyll in thermophilic cyanobacterium synechococcus elongatus.	stationary delayed fluorescence (df) of chlorophyll in isolated membrane preparations from thermophilic cyanobacterium synechococcus elongatus was investigated as a function of temperature. two peaks at different temperatures were observed. the low-temperature peak (54-60 degrees c) coincided with the main maximum of the thermally-induced delayed fluorescence of chlorophyll in intact cells and psii-particles with active oxygen-evolving system. the high-temperature peak (78 degrees c) coincided w ...	1992	1426973
evidence for natural transfer of a tetracycline resistance gene between bacteria from the human colon and bacteria from the bovine rumen.	previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, prdb5, from the human colonic anaerobe bacteroides uniformis to the ruminal anaerobe prevotella (bacteroides) ruminicola b(1)4. we have now shown that naturally occurring gene transfer elements in bacteroides species and prevotella ruminicola can also be transferred between these two genera. a self-transmissible chromosomal element originally found in a clinical isolate of bacteroides fragilis (tcr emr 12256 ...	1992	1599250
transfer of hybrid plasmids based on the replicon prri7 from escherichia coli to bacteroides and prevotella strains.	new shuttle vectors based on a prevotella ruminicola 9.5 kb cryptic plasmid (prri7) inserted within the escherichia coli vector pkc71, carrying the ccr/emr bacteroides marker, were constructed. these constructs (pkbr23-1 and pkbr23-2) were transferred into bacteriodes distasonis, bacteroides thetaiotaomicron, bacteroides uniformis and into p. ruminicola ncfb 2202 either by conjugal mobilization or by electroporation. another prri7 derivative based on pkc72, pkbr23-3, was smaller (13.1 kb) and no ...	1993	8486560
unexpected sequence similarity between nucleosidases and phosphoribosyltransferases of different specificity.	amino acid sequences of enzymes that catalyze hydrolysis or phosphorolysis of the n-glycosidic bond in nucleosides and nucleotides (nucleosidases and phosphoribosyltransferases) were explored using computer methods for database similarity search and multiple alignment. two new families, each including bacterial and eukaryotic enzymes, were identified. family i consists of escherichia coli amp hydrolase (amn), uridine phosphorylase (udp), purine phosphorylase (deod), uncharacterized proteins from ...	1994	7920254
cloning and expression of the bacteroides fragilis ych46 neuraminidase gene in escherichia coli and bacteroides uniformis.	a neuraminidase-encoding gene nanh of bacteroides fragilis strain ych46 was cloned into the cosmid vector phc79. the nanh gene was subcloned from the cosmid and was located within a 2.2-kb xhoi-kpni fragment. southern hybridization experiments demonstrated that the gene was present as a single copy on the bacterial chromosome. neuraminidase activity expressed in the initial escherichia coli clone was approximately 3600-fold lower than that expressed in b. fragilis ych46. however, when nanh was t ...	1994	7926664
molecular and genetic analysis of the bacteroides uniformis cephalosporinase gene, cbla, encoding the species-specific beta-lactamase.	the gene, cbla, encoding the species-specific, clavulanate-susceptible, endogenous cephalosporinase was cloned from bacteroides uniformis wal-7088. the nucleotide sequence was determined, and the cbla structural gene was found to be 891 nucleotides, with a 48% g+c composition, which is similar to that of the b. uniformis genome. the cbla open reading frame encoded an ambler class a beta-lactamase polypeptide precursor of 296 amino acid residues with a predicted molecular weight of 33,450. a beta ...	1994	7985999
gender difference in the risk factors for sudden cardiac death.	sudden cardiac death is most often caused by ventricular dysrhythmias. although women have an incidence of sudden death lower than that of men, 34% of coronary deaths in women are sudden deaths. the atherogenic risk factors do not predict which women are at the highest risk of sudden cardiac death. left ventricular hypertrophy is a strong risk factor for sudden cardiac death in women. asymptomatic ventricular dysrhythmias, a risk factor for death after myocardial infarction in men, do not increa ...	1994	2142895
purification and characterization of a new beta-lactamase from bacteroides uniformis.	a beta-lactam-resistant bacteroides uniformis strain was isolated from a clinical specimen. the strain produced large amounts of beta-lactamase and was resistant to penicillins and cephalosporins. the specific activity of the unpurified beta-lactamase was 4.8 u/mg of protein with nitrocefin as the substrate. the enzyme was purified 188-fold by q-sepharose, sephacryl s-300, and mono q column passages. kinetic parameters of the enzyme were determined by a micromethod performed in microtiter plates ...	1995	7492085
expression of a cloned cellulase/xylanase gene from prevotella ruminicola in bacteroides vulgatus, bacteroides uniformis and prevotella ruminicola.	a new shuttle vector, prh3 (8.7 kb), was constructed for use in prevotella/bacteroides host strains. this vector combines the prri2 replicon from p. ruminicola, pbluescript sequences and a tetq marker gene for selection in prevotella/bacteroides hosts. following insertion of a fragment carrying an endoglucanase/xylanase gene from p. ruminicola 23 into the multiple cloning site, the resulting construct, prh3x, was introduced into b. vulgatus 1447, b. uniformis 1100 and p. ruminicola 2202. this re ...	1995	7592134
effect of h2-receptor antagonists on bile acid metabolism.	several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ...	1995	7735920
a case of subacute necrotizing fasciitis.	we report a 48-year-old woman who developed necrotizing groin fasciitis with insidious onset. before she visited us, she had been unsuccessfully treated with several kinds of antibiotics by other doctors for one month, because of a small ulcer covered by blackish necrotic tissue. she was referred to us because of high fever, an ulcer on the left labium majus, and a cellulitis-like lesion with severe pain on the lower abdomen. methicillin-resistant staphylococcus aureus (mrsa), streptococcus inte ...	1995	8648007
use of a modified bacteroides-prevotella shuttle vector to transfer a reconstructed beta-1,4-d-endoglucanase gene into bacteroides uniformis and prevotella ruminicola b(1)4.	a carboxymethyl cellulase (cmcase) gene from prevotella ruminicola b(1)4 was reconstructed by adding a cellulose binding domain from a thermomonospora fusca cellulase and was conjugally transferred from escherichia coli to bacteroides uniformis 0061 by using a chloramphenicol and tetracycline resistance shuttle vector (ptc-cow). ptc-cow was specifically constructed to facilitate conjugal transfer of vectors from b. uniformis donors to p. ruminicola recipients. b. uniformis transconjugants contai ...	1996	8572695
characterization of saccharolytic bacteroides and prevotella isolates from infected dog and cat bite wounds in humans.	saccharolytic, nonpigmented, anaerobic gram-negative rods isolated from infected dog and cat bite wounds in humans have been poorly characterized, and most are not included in the databases of kits used for anaerobic identification; thus, they are problematic for clinical laboratories to identify. fifty strains isolated from such wounds were characterized with commercial kits for preformed-enzyme detection, carbohydrate fermentation, and other biochemical tests. pcr fingerprinting was performed ...	1997	9003606
stimulation of transforming growth factor beta1 by enteric bacteria in the pathogenesis of rat intestinal fibrosis.	bacteria and their products stimulate inflammatory responses. certain mediators, such as transforming growth factor beta1 (tgf-beta1), induce collagen synthesis. excess collagen deposition results in bowel strictures. the aim of this study was to investigate the role of bacteria and tgf-beta1 in the pathogenesis of intestinal fibrosis.	1998	9496942
[c-peptide, igg-insulin antibodies, island cell antibodies and immune response genes in diabetes mellitus].		1998	105488
total nose reconstruction: a single stage method.		1998	105856
jack tizard.		1999	89494
john frederick paterson.		1999	89445
vitiligo patterns simulating autoimmune and rheumatic diseases.	several variants of generalised vitiligo can be recognised by their cutaneous distribution. in some, certain anatomical regions--e.g., hands--are predominantly affected while the cutaneous depigmentation in other variants shows a similar anatomical distribution to that of the internal structures affected in some of the autoimmune and rheumatic diseases. five patients have been seen, and three described by others, with vitiligo affecting skin of eyelids and lower front of neck, reminiscent of the ...	1999	89452
fatal leishmaniasis in renal-transport patient.		1999	88649
in vitro activity of gemifloxacin (sb 265805) against anaerobes.	gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...	1999	10471570
use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites.	conditions for preparing immunoadsorbents of sperm-whale myoglobin and its five synthetic antigenic sites and for desorption of radiolabeled antibodies from the immunoadsorbents were studied. in immunoadsorbent titration studies, the sum of the amounts of antibodies bound in the plateau (maximum binding) by the adsorbents of the five sites accounted quantitatively for the entire (100%) antibody response to sperm-whale myoglobin.	1999	91647
monitoring drug use in a community.		1999	3250707