Publications

[dna base composition and taxonomic position of glucose-fermenting marine bacteria of the genus vibrio, and related genera].		1973	4707592
regulation of aspartokinase activity in the genus beneckea and marine, luminous bacteria.		1973	4709503
bacterial bioluminescence-identification of fatty acid as product, its quantum yield and a suggested mechanism.		1973	4712197
studies on yeast hemoglobin. the properties of yeast hemoglobin and its physiological function in the cell.		1973	4713242
intermediates in the bacterial luciferase reaction.		1973	4717759
studies on luciferase from photobacterium phosphoreum. iv. preparation and properties of stripped luciferase.		1973	4770374
[influence of the nature of support on radiation dosage near the marginal surfaces during irradiation with soft x-rays].		1973	4778523
[stability of the properties of luminescent bacteria stored for a long time on vaseline oil].		1973	4791165
conversion of aldehyde to acid in the bacterial bioluminescent reaction.		1973	4796920
[optimization of the nutrient medium composition for bacteria of the genus photobacterium].		1973	4799686
photolumazines, new naturally occurring inhibitors of riboflavin synthetase.		1973	4355564
the cyclic amp receptor of escherichia coli: immunological studies in extracts of escherichia coli and other organisms.		1973	4356533
control of in vivo luminescence in psychrophilic marine photobacterium.		1973	4360241
bioluminescence: fundamental and practical aspects.		1973	4127503
the chemistry of bioluminescence.		1973	4131760
extractable lipids of gram-negative marine bacteria: phospholipid composition.	phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. results showed that phospholipids of marine bacteria differed very little from those of nonmarine organisms with phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol being the predominant phospholipids in all strains examined. lyso-phosphatidylethanolamine occurred in significant quantities among a number of the marine bacteria, and two of the isolates contained significant quantities ...	1973	4197274
isolation, enumeration, and host range of marine bdellovibrios.		1974	4211210
enzymatic reduction of 5-deazariboflavine from reduced nicotinamide adenine dinucleotide by direct hydrogen transfer.		1974	4152873
flavin binding by bacterial luciferase: affinity chromatography of bacterial luciferase.		1974	4364563
mitochondrial functions under hypoxic conditions. the steady states of cytochrome c reduction and of energy metabolism.		1974	4366888
decyl nitrite: an aldehyde analog in the bacterial bioluminescence reaction.		1974	4826468
the binding and spectral alterations of oxidized flavin mononucleotide by bacterial luciferase.		1974	4830741
studies on luciferase form photobacterium phosphoreum. v. an enzyme-fmn intermediate complex in the bioluminescent reaction.		1974	4834652
effects of superoxide radicals on myoblast growth and differentiation.		1974	4834688
isolation of a new copper-containing superoxide dismutase bacteriocuprein.		1974	4836277
effect of sodium chloride on growth of heterotrophic marine bacteria.		1974	4837198
[studies in bioluminescence. 13. bacterial bioluminescence: demonstration and identification of the transformation product of aldehyde].		1974	4847069
natural antibodies and the intestinal flora of rodents.		1974	4606835
studies on luciferase from photobacterium phosphoreum. vi. stoichiometry and mode of binding of fmnh2 and o2 to stripped luciferase.		1974	4426891
iron containing superoxide dismutases from luminous bacteria.		1974	4451675
studies on luciferase from photobacterium phosphoreum. vii. interaction with carboxylic acid.		1974	4452671
the aldehyde content of luminous bacteria and of an "aldehydeless" dark mutant.	fatty aldehydes, present in the luminescent cells of photobacterium phosphoreum and achromobacter fischeri, and to a very slight extent in the cells of a visually dark, "aldehydeless" mutant of the latter species, were extracted, purified, and oxidized to the corresponding acids. the acids were analyzed by mass spectrometry. the results, in conjunction with various other lines of evidence, indicate that saturated fatty aldehydes, comprising mostly dodecanal, tetradecanal, and hexadecanal, functi ...	1974	4531008
effect of temperature on potency of anesthetic agents.		1974	4413098
luminescence and respiratory activities of photobacterium phosphoreum. competition for cellular reducing power.	changes in the in vivo luminescence, respiratory activities, contents of cytochromes, extractable luciferase and nad(p)h-fmn reductase during growth of the wild (bright) strain of photobacterium phosphoreum and its dim mutant were determined. the intensity of the in vivo luminescence per cell increased 10 times in the wild strain and 750 times in the dim strain during logarithmic growth, while the contents of luciferase and nad(p)h-fmn reductase remained almost constant. it is suggested that a c ...	1975	5397
flavin mononucleotide reductase of luminous bacteria.	nad(p)h: fmn oxidoreductase (flavin reductase) couples in vitro to bacterial luciferase. this reductase, which is also postulated to supply reduced flavin mononucleotide in vivo as a substrate for the bioluminescent reaction, has been partially purified and characterized from two species of luminous bacterial. from photobacterium fischeri the enzyme has a m. w. determined by sephadex gel filtration, of 43,000 and may have a subunit structure. the turnover number at 20 degrees c, based on a purit ...	1975	47604
proceedings: evidence for specific nadh- and nadph-fmn reductases in bacterial bioluminescence.		1975	54079
enzymes of d-fructose catabolism in species of beneckea and photobacterium.	cell-free extracts of strains representative of the genera beneckea and photobacterium catalyzed a p-enolpyruvate dependent phosphorylation of d-fructose. the resulting product, fructose-1-p, was converted to fructose-1,6-p2 by 1-p-fructokinase. both activities were inducible, being present in d-fructose-grown cells and reduced or absent in d-gluconate-(or succinate-) grown cells.	1975	125566
blue--green bacteria: status and photoqutotrophic metabolism.		1975	236953
ferredoxins, blue-green bacteria and evolution.		1975	236955
bioluminescence: from chemical bonds to photons.	the biological transformation of chemical to photic energy involves an enzyme-mediated chemiluminescent reaction, in which one of the products exists in an electronically excited state, emitting a photon as it returns to the ground state. the colour of bioluminescence differs in different organisms, ranging from the deep blue (460 nm) of certain crustacea, through the bluish green (490 nm) of some bacteria, the green (530 nm) of mushrooms to the red (about 600 nm) of the railroad worm. in one ca ...	1975	238805
5s rna secondary structure.		1975	808733
bacterial luciferase requires one reduced flavin for light emission.	recent reports revive a hypothesis that the bacterial bioluminescence reaction involves two reduced flavin mononucleotide molecules per enzyme turnover. a two-flavin mechanism requires that the two flavins bind simultaneously or sequentially to the same or different sites on luciferase during a catalytic cycle. measurements using equilibrium techniques show that the luciferase dimer has only a single reduced flavin binding site. quantum yield results demonstrate that bioluminescence requires onl ...	1975	1059124
the continuous culture of luminous bacteria: a luminostat.		1975	1107290
[cellular protrusions of luminescent bacteria and relation of their appearance to cultivation conditions].	the luminescent cells of photobacterium were studied by electron microscopy in order to establish what caused their sticking together and growth on the glass walls of the vessel during continuous cultivation. electron microscopy revealed protrusions, of various shape and size, on the cells. the phenomenology of various protrusions, including fimbria, is described, and the effect of cultivation conditions (continuous culture, periodic culture) and growth phases on their emergence was elucidated. ...	1975	1160626
identification of nadh-specific and nadph-specific fmn reductases in beneckea harveyi.	distinct fmn reductases specific for nadh and nadph were identified in extracts of beneckea harveyi. these enzymes differ in their physical (molecular weight, thermostability) as well as in their chemical properties (binding constants for nadh and nadph). the nadh-specific enzyme is more efficient than the nadph-specific one with respect to the bioluminescent reaction.	1975	1175652
[impulse characteristics of bacterial bioluminescence].		1975	1183308
mechanism of inhibition of bacterial luciferase by anaesthetics.		1975	1219299
[deoxyribonucleic acid hybridizations among some vibrio-like marine bacteria (author's transl)].	the genotypic relationships established by dna/dna hybridization in vitro confirm the results obtained by earlier phenotypic analyses of certain vibrio-like marine bacteria. these strains are closely related to the species photobacterium fischeri, and do not belong to the genus vibrio. the group of marine, vibrio-like bacteria that require na+ for growth is genotypically very heterogeneous.	1975	1190947
the nucleotide sequence of the 5s ribosomal rna from a photobacterium.	comparative sequencing studies provide powerful insights into molecular function and evolution. the sequence for 5s ribosomal rna from photobacter strain 8265 is eighteen base replacements removed from that of escherichia coli. of these, the vast majority involve a g or c becoming an a or u. these variations also define unequivocally a hexanucleotide base paired region, which appears to be a universal feature of the 5s rna molecule. the base composition of this helix seems to be under rather str ...	1975	1177325
[osmiophilic formations in cells of luminous bacteria].		1975	1140069
bacterial bioluminescence: equilibrium association measurements, quantum yields, reaction kinetics, and overall reaction scheme.	the characteristics of the bioluminescence reactions with bacterial luciferase from two different cell types, photobacterium fischeri and beneckea harveyi, are reported. the reduced flavine mononucleotide (fmnh2)-luciferase association constant, directly measured by equilibrium dialysis and gel filtration is the same for both luciferases, 3 times 10(-4) mminus1 at romm temperature, and is significantly different from the kinetic reciprocal michaelis-menten constant. the luciferase bioluminescenc ...	1975	807236
[biochemical bases of bioluminescence].		1975	235818
symbiotic association of photobacterium fischeri with the marine luminous fish monocentris japonica; a model of symbiosis based on bacterial studies.	isolation of bacteria from the luminous organ of the fish monocentris japonica has revealed that the organ contains a pure culture of luminous bacteria. for the four fish examined, all contained photobacterium fischeri as their luminous bacterial symbiont. this is the first time that p. fischeri has been identified in a symbiotic association. a representative isolate (mjl) of the light organ population was selected for in vivo studies of its luminous system. several physiological features sugge ...	1976	1016667
studies on luciferase from photobacterium phosphoreum. viii. fmn-h2o2 initiated bioluminescence and the thermodynamics of the elementary steps of the luciferase reaction.	bacterial luciferase from photobacterium phosohoreum was found to produce bioluminescence on reaction with fmn and h2o2 in the presence of aldehyde. this luminescence is presumably produced by the same x1 intermediate as that found in fmnh2-o2 initiated luminescence. from the ratio of the light intensities of the fmn-h2o2 initiated reactions, we calculated the association constant of the reaction, luciferase+fmn+h2o2in equilibriumx1, and estimated its temperature dependence. from these results w ...	1976	950335
study of genetic relationships among marine species of the genera beneckea and photobacterium by means of in vitro dna/dna hybridization.	strains representative of species of the marine genera beneckea and photobacterium were used as reference standards in in vitro dna/dna competition experiments. within a given species, strains were found to be related by over 80% competition. (competition was defined as the amount of radioactive dna displaced by heterologous dna relative to the amount displaced by homologous dna.) on the basis of interspecies competition values (expressed as averages), the following groupings could be made: 1. " ...	1976	1015934
[interaction of dexamethasone-receptor complexes with rat liver nuclei and dnas].	the role of dnas in the nuclear binding of dexamethasone-receptor complexes (drc) was studied. the cytosolic receptors from rat liver have a sedimentation coefficient of about 7s, the stock's radius--of about 50 a and possess a high affinity to dexamethasone (kas = 2,6 x 10(8) m-1). their capacity is 3 x 10(-13) and 5.5--7.0 x 10(-12) mole of dexamethasone per mg cytosolic protein and mg dna, respectively. drc has the ability to bind to the nuclei of rat liver. drc binding to nuclei is increased ...	1976	1022278
studies on luciferase from photobacterium phosphoreum. ix. further studies on the spectroscopic characteristics of the enzyme-fmn intermediates.	the absorption and fluorometric changes of the reaction mixture of luciferase-fmnh2 complex with o2 were re-examined. rapid formation (k2(app) = 2.0 s-1 at [o2] = 120 micrometer) of an intermediate with a single absorption maximum at 380 nm within the range of 350-550 nm, and a weak fluorescence at 520 nm (less than or equal to 10% of that of fmn when excited at 380 nm) was observed. the absorption and fluorescence spectra and decay rate of the intermediate were estimated by simulation using an ...	1977	881410
a luminous bacterium that emits yellow light.	a strain of photobacterium fischeri that emits yellow light has been isolated from seawater. the bimodal spectrum, which is unique among the luminous bacteria, consists of a major band with a maximum at 545 nanometers and a minor band with a maximum at 500 nanometers. the former represents a heretofore unreported range of emission for luminous bacteria, while the latter coincides with the emission spectrum of typical blue-greeen-emitting strains of p. fischeri. the relative contributions of thes ...	1977	850787
low oxygen is optimal for luciferase synthesis in some bacteria. ecological implications.	the synthesis of the bioluminescent systems in many strains of two species of the genus photobacterium which were isolated as symbionts is greater at low oxygen concentrations, where aerobic growth is blocked. in strains of two other species, one photobacterium of symbiotic orgin, and one (genus beneckea) whose lent response is observed. at low oxygen concentrations, where there is an inhibition of growth, there is also a similar decrease in the synthesis of the luminescent system. these species ...	1977	843171
autoinduction of bacterial luciferase. occurrence, mechanism and significance.	the synthesis of the luminous system of the marine luminous bacterium photobacterium fischeri is subject to a complex, self-regulated control system called autoinduction. the bacteria produce an autoinducer which accumulates in the medium at a constant rate (as a function of cell growth). when autoinducer reaches a critical concentration it stimulates, at the level of transcription, the synthesis of the luminous system. autoinduction is thus viewed as an environmental sensing mechanism, which cu ...	1977	843170
pyruvate production and excretion by the luminous marine bacteria.	during aerobic growth on glucose, several species of luminous marine bacteria exhibited an imcomplete oxidative catabolism of substrate. pyruvate, one of the products of glucose metabolism, was excreted into the medium during exponential growth and accounted for up to 50% of the substrate carbon metabolized. when glucose was depleted from the medium, the excreted pyruvate was promptly utilized, demonstrating that the cells are capable of pyruvate catabolism. pyruvate excretion is not a general p ...	1977	303077
luminescence and respiratory activities of photobacterium phosphoreum. ii. control by monovalent cations.	luminescent activity of spheroplasts of the cells of photobacterium phosphoreum was stimulated by rb+ and k+ and inhibited by na+ in the medium. opposite effects of these ions were observed on the rate of o2 consumption of the spheroplasts through the cytochrome and luciferase electron transfer systems. in vitro activities of nadh-fmn reductase and luciferase were only slightly stimulated by rb+, k+, and na+, while na+ exhibited significant activation of the nadh-oxidizing activity of the cells. ...	1977	599151
characterization of some fish and shrimp spoiling bacteria.	the classification of some important groups of bacteria involved in fish and shrimp spoilage was studied. trimethylamine is produced by pseudomonas putrefaciens, a "non-defined" group resembling ps. putrefaciens, photobacterium spp. and some moraxella-like bacteria. hypoxanthine is produced by the same groups of bacteria except the last named and also by the "typical shrimp spoilers" (presumptive alteromonas). strong off-odours are produced on fresh fish by ps. putrefaciens, dextrose-oxidative p ...	1977	341802
purification and properties of the nadh and nadph specific fmn oxidoreductases from beneckea harveyi.	the nadh and nadph specific fmn oxidoreductases from beneckea harveyi have been purified to homogeneity as judged by single bands on sodium dodecyl sulfate gel electrophoresis. the overall purification for the nadh specific enzyme is 3000-fold and 4000-fold for the nadph specific enzyme from a crude extract. the final step in the purification procedure is chromatography on a 5'-amp-sepharose 4b affinity column which results in approximately a 50-fold purification to a final specific activity of ...	1977	880288
the isolation of a bacterial glycoprotein with luciferase activity.		1977	900939
chemical modification studies on a ferri-superoxide dismutase from marine bacteria.		1977	913572
control of luciferase synthesis in a newly isolated strain of photobacterium leiognathi.	in previous studies with luminous bacteria of all different species it has been reported that the synthesis of luciferase is autoinducible: during growth at low cell densities synthesis is effectively repressed while after induction, at higher cell densities, the rate of synthesis of enzyme is up to five times the growth rate. in this paper we report on newly isolated strains of photobacterium leiognathi which show continued luciferase synthesis irrespective of the cell density. the specific syn ...	1977	603341
an experimental model of the krogh tissue cylinder: two dimensional quantitation of the oxygen gradient.		1977	613755
reaction rate theory in bioluminescence and other life phenomena.		1977	326144
biology of the marine enterobacteria: genera beneckea and photobacterium.		1977	334043
bacterial bioluminescence.		1977	199107
a pulse-radiolysis study of the catalytic mechanism of the iron-containing superoxide dismutase from photobacterium leiognathi.	the mechanism of the enzymic reaction of an iron-containing superoxide dismutase purified from the marine bacterium photobacterium leiognathi was studied by using pulse radiolysis. measurements of activity were done with two different preparations of enzyme containing either 1.6 or 1.15 g-atom of iron/mol. in both cases, identical values of the second-order rate constant for reaction between superoxide dismutase and the superoxide ion in the ph range 6.2-9.0 (k=5.5 x 10(8) m-1-s-1 at ph 8.0) wer ...	1977	15540
a calorimetric investigation of the growth of the luminescent bacteria beneckea harveyi and photobacterium leiognathi.	direct calorimetric determinations of the rate of heat production along with simultaneous determinations of the rate of photon emission and the number of viable cells have provided insight into the growth of beneckea harveyi and photobacterium leiognathi. these experiments were performed with a tronac isothermal microcalorimeter modified with a fiber optic light guide to allow in situ detection of light. escherichia coli and a dark variant of p. leiognathi were also examined to provide points of ...	1977	401656
electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes.		1978	202508
separation of a blue fluorescence protein from bacterial luciferase.		1978	623648
studies on luciferase from photobacterium phosphoreum. x. heat of formation of the intermediate in the bioluminescent reaction studied by stopped-flow calorimetry.	the heat production in the reaction of luciferase-fmnh2 complex with o2 in the absence of aldehyde was measured by stopped-flow calorimetry. deltah of the reaction, luciferase-fmnh2+ o2 leads to intermediate x1, is -1.3 x 10(2) kj.mol-1 and the calculated deltas for the reaction is -180 j.mol-1.k-1 at 20 degrees c. the heat production in the bioluminescent reaction was also measured in the presence of a saturating concentration of aldehyde, and it was estimated that 43 and 79% of the c10 and c13 ...	1978	659382
[luminescence and growth of photobacterium mandapamensis in periodic culture].	the luminescent photobacterium mandapamensis, strain 54 (the collection of the institute of physics, siberian branch of the ussr academy of sciences), was isolated from the water of the pacific ocean in the equatorial zone and studied in the course of periodic cultivation. the dynamics of changes in the main parameters of the culture, such as luminescence, growth, respiration, heat emission etc., was investigated. the data obtained were used to establish a correlation between changes in these pa ...	1978	713875
sensitive oxygen assay method by luminous bacteria.		1978	732582
activity and stability of the luciferase--flavin intermediate.	a luciferase intermediate in the bacterial bioluminescence system, which is formed by reaction of enzyme with reduced flavin mononucleotide (fmnh2) and oxygen, is shown to emit light with added aldehyde under anaerobic conditions. the reaction with oxygen is thus effectively irreversible under the conditions used. the flavin chromophore has an absorption maximum at about 370 nm and the potential activity (bioluminescence yield) in the further reaction of the isolated intermediate with aldehyde i ...	1978	306832
properties and kinetics of salt activation of a membrane-bound nadh dehydrogenase from a marine bacterium photobacterium phosphoreum.	a membrane-bound nadh dehydrogenase, solubilized and partially purified from a marine bacterium photobacterium phosphoreum, contains fad as the prosthetic group, and is specific for nadh. ferricyanide, various other redox dyes and cytochrome c can act as electron acceptors. the enzymatic activity when assayed with electron acceptors other than cytochrome c, is activated by monovalent cations (na+ and k+) and deactivated by high concentrations of monovalent anions (scn-, no3-, and cl-) but not by ...	1978	721793
myristic acid stimulation of bacterial bioluminescence in "aldehyde" mutants.	the involvement of long chain aldehyde in bacterial luminescence was known both from its being required for light emission in the in vitro reaction with pure luciferase and from its ability to stimulate luminescence in vivo in a certain class of dark "aldehyde" mutants. we have found that the luminescence of some (but not all) of such aldehyde mutants is also stimulated by long chain aliphatic fatty acids, with a marked specificity for myristic (tetradecanoic) acid. this stimulation has been dem ...	1978	24214
studies on luciferase from photobacterium phosphoreum. xi. interaction of 8-substituted fmnh2 with luciferase.	the interaction of bacterial luciferase from photobacterium phosphoreum with reduced flavin was investigated using various 8-substituted fmnh2 analogs. flavins tested were fmnh2 and fmnh2 substituted at the 8 position with ho-, ch3o-, c2h5o-, cl-, br-, i-, h2n-, (ch3)hn-, and (ch3)2n. 8-ch30-, c2h5o-, cl-, and br-fmnh2 showed luminescent activity in the luciferase reaction with emission peaks at various wavelengths. 8-ho- and i-fmnh2 were competitive inhibitors toward fmnh2 in the luminescent re ...	1978	738995
[isolation of bacterial luminescence reaction inhibitor from photobacterium sp. cells].	the factor having a strong inhibitory effect on bacterial luminescence was isolated from the luminous bacteria species photobacterium sp. the inhibitor purified by gel filtration on the biogel and by deae chromatography was homogenous (single bound during electrophoresis in polyacrylamide gel), it reacted with coomassie brilliant blue and gave a positive lowry reaction on protein. molecular weight was about 30,000 as determined by sds-polyacrylamide gel electrophoresis. the absorption spectrum w ...	1978	737225
superoxide dismutases: defence against endogenous superoxide radical.	attempts to measure the rate of o2- production, in whole cells or in intact subcellular organelles, are frustrated by the endogenous superoxide dismutase (sod). streptococcus faecalis contains a single manganese-sod which was isolated and used as an antigen in the rabbit. a precipitating and inhibiting antibody was obtained and used to suppress the sod in crude lysates of s. faecalis. it allowed the demonstration that 17% of the total oxygen uptake by such lysates, in the presence of nadh, was a ...	1978	225147
isolation of the in vivo emitter in bacterial bioluminescence.	a blue fluorescence protein has been isolated and purified from extracts of the luminous bacterium photobacterium phosphoreum. it is a single polypeptide of molecular weight 22,000 with absorption maxima at 274 and 418 nm. it is efficiently fluorescent (varphi(f) 0.45), with a fully corrected spectral maximum (476 nm) and distribution identical to the in vivo bioluminescence from this same type of bacterium. at low concentration this fluorescence shifts towards the red and becomes identical to t ...	1978	16592497
separation and structure of the prosthetic group of the blue fluorescence protein from the bioluminescent bacterium photobacterium phosphoreum.	the highly fluorescent prosthetic group of the blue fluorescence protein purified from the bioluminescent bacterium photobacterium phosphoreum has been dissociated and separated from its apoprotein by affinity chromatography on cibacron blue-sepharose. it has been identified as 6,7-dimethyl-8-(1'-d-ribityl)lumazine by several methods of characterization, all of which gave results identical to those for an authentic sample. in neutral solution, absorption maxima are at 407, 275 (shoulder), and 25 ...	1979	16592674
seasonal and geographic distribution of luminous bacteria in the eastern mediterranean sea and the gulf of elat.	luminous bacteria in the mediterranean sea and the gulf of aqaba-elat have different distribution patterns. in the mediterranean sea, beneckea harveyi is present all year round, with different subtypes alternating in summer and winter; photobacterium fischeri was only present during the winter. in the gulf of elat, p. leiognathi is present throughout the water column in similar densities during the entire year. this constancy in distribution is presumably due to the near-constancy in water tempe ...	1979	16345404
physiological characteristics underlying the distribution patterns of luminous bacteria in the mediterranean sea and the gulf of elat.	physiological characteristics of luminous bacteria isolated from the mediterranean and gulf of elat were compared to determine their relationship to the specific seasonal and geographic distribution patterns of these bacteria. the effects of temperature on growth rate and yield, relative sensitivity to photooxidation, resistance to high salt concentration (8%), and ability to grow in nutrient-poor conditions appear to control these patterns. the winter appearance of photobacterium fischeri and t ...	1979	16345442
luminous enteric bacteria of marine fishes: a study of their distribution, densities, and dispersion.	three taxa of luminous bacteria (photobacterium fischeri, p. phosphoreum, and beneckea spp.) were found in the enteric microbial populations of 22 species of surface- and midwater-dwelling fishes. these bacteria often occurred in concentrations ranging between 10 and 10 colony-forming units per ml of enteric contents. by using a genetically marked strain, it was determined that luminous cells entering the fish during ingestion of seawater or contaminated particles traversed the alimentary tract ...	1979	16345429
luminescence in clinical analysis.		1979	231928
applications of bio- and chemiluminescence in the clinical laboratory.		1979	380839
the terminal oxidase of photobacterium phosphoreum. a novel cytochrome.	the terminal oxidase of photobacterium phosphoreum has been purified to the electrophoretically homogeneous state and some of its properties have been studied. the enzyme catalyses oxidation of ascorbate in the presence of phenazine methosulphate or n,n,n',n'-tetramethyl-p-phenylenediamine. the reaction is inhibited by cyanide. nitrite at comparatively high concentrations inhibits the enzyme, but the enzyme does not catalyse nitrite reduction with ascorbate plus the electron mediator as the elec ...	1979	465487
covalent structure of subunits of bacterial luciferase: nh2-terminal sequence demonstrates subunit homology.	the heterodimeric subunit structure of bacterial luciferase was demonstrated more than 10 years ago. the enzymes from both beneckea harveyi and photobacterium fischeri have since been studied in detail; they each consist of two nonidentical subunits, designated alpha and beta. both are required for bioluminescence activity, with the active center apparently confined to the alpha subunit. amino acid sequence analysis of the nh2 termini of the alpha and beta subunits of the b. harveyi and p. fisch ...	1979	315557
kinetic studies on the mechanism of bacterial nad(p)h:flavin oxidoreductase.		1979	222213
biosynthesis of aliphatic aldehydes for the bacterial bioluminescent reaction: stimulation by atp and nadph.		1979	223549
interaction of the mannosephilic lectins of pseudomonas aeruginosa with luminous species of marine enterobacteria.	the marine bacteria beneckea harveyi and photobacterium leiognathi were shown to bear mannose-containing binding sites for the mannosephilic lectins of pseudomonas aeruginosa and concanavalin a (con a). the interaction between the lectins and the marine bacteria was demonstrated by the bacteriagglutination test, by adsorption of the lectins onto the bacteria and by mannose-specific peroxidase-binding to the lectin-coated bacteria. treatment of the bacteria with formaldehyde, phenol, ethanol or b ...	1979	120927
high molecular weight blue fluorescence protein from the bioluminescent bacterium photobacterium fischeri.		1979	435324
[genetic studies of photobacterium mandapamensis. ii. the classification of mutants with an altered luminescence intensity according to their sensitivity to exogenous aldehyde].	the collection of 157 dark and dim photobacterium mandapamensis strains was divided into four groups using the addition of 0.2 ml of 0.15% myristis aldehyde to cell suspension. the luminescence did not change in the presence of the aldehyde in 76 mutants, it decreased in 30 mutants, it was 2--8-fold increased in 35 mutants, and it was increased more than 10-fold in 16 mutant strains. 19 strains of those having luminescence in the presence of the aldehyde have mutations in genes controlling the b ...	1979	488711
[determination of trace amounts of 2,4-dinitrofluorobenzene by a bioluminescence method].		1979	508879
[ultrastructural organization of marine luminescent bacteria and their mutants].	the aim of this work was to study the submicroscopic organization of luminescent bacteria belonging to the genera photobacterium and lucibacterium as well as that of their "dark" mutants incapable of luminescence. the ultrastructural organization of all studied bacteria is typical of gram-negative species. the luminescent bacteria are characterized by the presence, in their cytoplasm, of osmophilic formations 22--110 nm in size. the cells of "dark" mutants accumulate volutin and contain complex ...	1979	530133