Publications

isolation and characterization of a high molecular weight glycoprotein from human blood platelets.	a high molecular weight glycoprotein consisting of three disulfide-linked 142,000 molecular weight chains has been isolated from human blood platelets. the glycoprotein, designated thrombospondin, is released by platelets in response to thrombin treatment and is proteolyzed when left in the presence of platelets after liberation. it is relatively insensitive to degradation by thrombin. thrombospondin is a filamentous protein of dimensions approximately 7 x 70 nm and contains 1.9% neutral sugars, ...	1978	101549
[clinical studies of childhood empyema experienced for the past 12 years (author's transl)].		1978	101626
effect of hypotension induced by sodium nitrocyanoferrate (iii) on the release of arginine-vasopressin in the unanaesthetized monkey.		1978	101639
plasmid borne virulence of enterobacter hafniae.	the strain classified as enterobacter hafniae has been isolated in a severe epidemy of porcine diarrhoea. its pathogenicity has been found to be cotransmissible with resistance to oxytetracycline when the strain was grown in a mixed culture with some nonpathogenic e. hafniae strains as well as with escherichia coli. toxinogenity seems to be responsible for virulence of the strain.	1978	76419
purification and assay of murine leukemia viruses.		1979	85237
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.	amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...	1979	87374
moloney virus-induced cell surface antigens and histocompatibility antigens are located on distinct molecules.		1979	87439
passive transfer in diabetes mellitus.		1979	88629
modulation of cellular interactions between c3h/10t1/2 cells and their transformed counterparts by phosphodiesterase inhibitors.	it has been demonstrated previously that nontransformed c3h/10t1/2cl8 mouse embryo fibroblasts (10t1/2) can induce a state of reversible growth inhibition in cocultured malignantly transformed mouse fibroblasts and that this inhibition is modulated by serum concentration. the present study suggests that cyclic nucleotides may be implicated in this intercellular communication. the phosphodiesterase inhibitors theophylline, caffeine, and 3-isobutyl-1-methylxanthine (ibx) at concentrations of 10(-3 ...	1979	89899
alpha-fetoprotein as a carrier protein in plasma and its bilirubin-binding ability.	the bilirubin-binding ability of human alpha-fetoproteins, which were purified from fetal cord serum and from ascites fluid of a hepatoma-bearing patient, was examined by the difference spectrum and the jacobsen peroxidase methods. the difference spectrum observed as a result of the specific binding of bilirubin to alpha-fetoprotein had a maximum at 482 nm, and this pattern was quite similar to that observed for serum albumin. the result obtained by the difference spectrum method showed that 1 m ...	1979	89900
the indirect rat mast cell degranulation test reconsidered.	the indirect rat mast cell degranulation test for the detection of human reaginic antibodies was reinvestigated. when the mast cells were incubated with human reaginic serum at concentrations exceeding approximately 3%, a massive, non-specific histamine release occurred, irrespective of the presence or absence of allergen. at tolerated serum concentrations (below 3%), the allergen concentration did not influence the release process. we conclude that the indirect rat mast cell test is not applica ...	1979	90655
connectivity of the auditory forebrain nuclei in the guinea fowl (numida meleagris).	injection of tritiated leucine and proline into the nucleus ovoidalis of the guinea fowl (numida meleagris) produces terminal labeling in the palaeostriatum and in three adjacent zones (field l1-l3) of the auditory neostriatum (an). l2, situated between l1 and l3, receives the main input and corresponds to the former field l of rose. these neuroanatomically defined zones of the auditory neostriatum are also characterized by differing properties of their neurons. injection of radioactive material ...	1979	91442
occurrence of beta 2-microglobulin in mammalian lymphocytes and erythrocytes.	cell-associated beta 2-microglobulin (beta 2m) has been studied by quantitative techniques in lymphocytes and erythrocytes of guinea pig, rabbit, rat and man. beta 2m was solubilized by sonication followed by treatment with acid, deoxycholate or thiocyanate, and then determined by radioimmunoassay. the average number of beta 2m molecules per lymphocyte, estimated after acidification, varied between 0.89 x 10(5) and 7.1 x 10(5). rat erythrocytes contained 3.0 x 10(3) molecules per cell, whereas n ...	1979	91519
morphochemic studies on histamine and monoaminooxidase in the liver and studies on histaminolytic activity of guinea pig plasma in the course of sensitization with heterogenic protein.		1979	91564
antigen specific t cell-mediated suppression. vi. properties of in vitro generated l-glutamic acid60-l-alanine30-l-tyrosine10 (gat)-specific t-suppressor factor(s) (gat-tsf) in responder mouse strains.		1979	91643
olivocerebellar projections in the cat studied by means of anterograde axonal transport of labelled amino acids as tracers.		1979	92769
selective aromatic-hydrophobic binding and fractionation of immunoglobulin by means of phenyl-(ch2)n-nh-substituted agaroses.		1979	92988
a further member of the yucaipa group isolated from the common wren (troglodytes troglodytes).	in 1977, 477 small birds of 52 species were virologically examined. five cloacal swabs from troglodytes troglodytes yielded two paramyxovirus strains which were found antigenically related to, but not identical with ch/yucaipa/california/56 virus according to three serological tests. the ecological significance of these findings and the possibility of paramyxovirus classification into four types are discussed.	1979	94770
[immunological problems in oncology (author's transl)].	the authors, after a view of the problems of immunology, regarding the most recent discoveries, deal with the conclusions about the immunological reactions in research and clinical oncology. many specific antibodies, formed as a result of the tumor, have been discovered in the field of research. it is impossible to say the same about human tumors and the probable hypothesis are numerous. the results until now obtained, even if they don't give a definite response, show that the immune defense of ...	1979	95475
a receptor for polymerized human and chimpanzee albumins on hepatitis b virus particles co-occurring with hbeag.	a receptor for polymerized human serum albumin was demonstrated on dane particles as well as on 20-nm hepatitis b surface antigen particles, isolated from asymptomatic carriers of hepatitis b virus who were positive for hbeag. in contrast, such receptor was not born by 20-nm hepatitis b surface antigen particles obtained from carriers positive for antibody to hbeag. hepatitis b surface antigen particles with the receptor were heavier than those without, and when treated with pronase, they became ...	1979	103774
transcription and translation in a pleiotropic streptomycin-resistant mutant of escherichia coli.	the role of the ribosomal protein s12 (streptomycin protein) in ribosome function and in other metabolic processes in the cell has been investigated. a spontaneous streptomycin-resistant strain of escherichia coli (sm3) carrying a mutation in the rpsl gene is deficient in its ability to induce the synthesis of the enzyme bets-galactosidase. it was demonstrated that the reduced rate of enzyme synthesis results from deficiencies in both the transcription of the lactose operon and translation of th ...	1979	104958
demonstration of the intermediate cells of billingham and medawar in the epidermis of macaca mulatta and rhesus monkey.	the digest of the pure epidermis preparation from macaca mulatta demonstrated three types of dendritic cells i.e. (1) dopa-positive melanocytes, (2) dopa-negative langerhans' cells and (3) weakly dopa-positive intermediate cells of billingham and medawar. the last type of cell was found to have characteristics being intermediate between those of the melanocytes and of the langerhans' cell. a similar digest prepared from the epidermis of the rhesus monkey demonstrated isomorphous dendritic cells ...	1979	106619
renal emphysema of the tranplanted kidney: sonographic appearance.		1979	106703
circadian control of thermoregulation in the squirrel monkey, saimiri sciureus.	the characteristics and control of the circadian rhythms of core body temperature (colonic) and skin temperature (tail) were studied in chair-acclimatized squirrel monkeys (saimiri sciureus). when animals were entrained to a light-dark cycle (12 h 600 lx; 12 h less than 1 lx) these two temperatures displayed prominent, reproducible, tightly coupled circadian rhythms. in contsant light of 600 lx, where no other effective circadian time cues were present, both temperature rhythms persisted with fr ...	1979	106737
reproductive capacity of rhesus monkeys following bilateral ovarian x-irradiation.	the effect of ovarian x-irradiation of the rhesus monkey upon menstrual cycle length, duration of menstrual bleeding, excretion of gonadotrophin in the urine, concentration of gonadotrophin in the pituitary gland, ovarian histology, and breeding performance was investigated. doses below 4000 r had no significant effect upon the above criteria. by contrast, doses of 4000 to 7000 r rapidly induced amenorrhoea in most animals, but unexpectedly these animals did not excrete greater than normal amoun ...	1979	108398
severe mycoplasma pneumoniae infection in otherwise healthy siblings.		1979	115983
biologic properties of lyophilized live typhoid vaccine strains.		1979	262514
guinea pig lung lavage cells after intranasal bcg sensitization.	recent studies have suggested that intranasal administration of antigen can induce local cell-mediated immunity in lung lavage cells. the present study was designed to examine the changes in composition of lung lavage cells and their capacity to produce the lymphokine migration inhibitory factor after intranasal immunization with bcg in guinea pigs. results indicate that guinea pigs responded to respiratory tract bcg infection with an increase in immunocompetent cells in the bronchoalveolar trac ...	1979	387595
differentiation of selected enterobacteriaceae by pyrolysis-gas-liquid chromatography.	pyrolysis-gas-liquid chromatography was used to differentiate selected species of enterobacteriaceae. individual cultures of salmonella typhi, hafnia alvei, and proteus vulgaris, and 12 strains of yersinia enterocolitica were grown in nutrient broth. after harvest and lyophilization, the bacterial samples were pyrolyzed at 900 degrees c, and their volatile fractions were separated on a 50-m capillary column coated with carbowax 20m. the resulting pyrolysis elution patterns (pyrograms) of the fou ...	1979	393169
an xyy man.	this case report describes a young man with a 47, xyy karyotype who was convicted of arson. he suffered from a cardiac disorder which may well have been part of the xyy syndrome rather than a chance association. his abnormal karyotype was disclosed in court and used by the defence in a plea in mitigation.	1979	497632
spinal analgesia and sedation of goats with lignocaine and xylazine.	spinal anaesthesia of 20 does undergoing laparotomies was accomplished with epidural injections of 2 per cent lignocaine at a dosage of 1 ml per 4.55 kg in 16 goats. epidural injection resulted in paralysis lasting 198.5 minutes +/- 36.6 minutes. subarachnlid injection resulted in paralysis lasting 180.8 minutes +/- 44.9 minutes. xylazine was given intramuscularly at the dosage of 0.11 mg per kg after posterior paralysis was complete. these treatments resulted in effective analgesia for surgical ...	1979	516312
[diagnostic value of the acid production from galacturonate to differentiate strains of "salmonella" sub-genus i and monophasic sub-genus iii from the other "salmonella" strains belonging to sub-genera ii, diphasic iii, iv, "citrobacter" and "hafnia alvei" (author's transl)].	two methods were compared: (1) the ability to use sodium glacturonate as source of carbon and energy in defined medium; (2) the ability to produce acid by fermentation of this substrate in peptone water with phenol red. results of growth on, acid formation from, galacturonate are identical. salmonella strains of sub-genus i and monophasic serotypes of sub-genus iii do not use galacturonate. salmonella of sub-genera ii and iv as well as diphasic serotypes of sub-genus iii use galacturonate. glact ...	1979	533071
proton translocation in cytochrome-deficient mutants of escherichia coli.	cytochrome-deficient cells of a strain of escherichia coli lacking 5-amino-levulinate synthetase have been used to study proton translocation associated with the reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase region of the electron transport chain. menadione was used as electron acceptor, and mannitol was used as the substrate for the generation of intracellular nadh. the effects of iron deficiency on nadh- and d-lactate-menadione reductase activities were studied in iron-deficie ...	1979	154508
electron microscopy of synaptonemal complexes in semithin sections.	the technique of osmium tetroxide fixation followed by a treatment with p-phenylenediamine was applied to mouse testes in order to analyze pachytene nuclei. observation of epon semithin sections (0.25--0.5 micrometer) in conventional electron microscopy revealed the presence of positively stained synaptonemal complexes surrounded by unstained chromatin. the present results show that by using this method a suitable contrast and resolution can be achieved for semithin sections, which facilitates t ...	1979	156471
[interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)].	gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ...	1980	6104464
evaluation of the four-hour micro-id technique for direct identification of oxidase-negative, gram-negative rods from blood cultures.	a 4-h micro-id technique for direct identification of oxidase-negative gram-negative rods from positive blood cultures was compared to subculture and species identification of single colonies by api 20e and micro-id, using standardized inocula. a total of 127 patients (220 positive cultures) were studied. isolates included 96 escherichia coli, 46 klebsiella pneumoniae, 7 klebsiella oxytoca, 8 enterobacter aerogenes, 17 enterobacter cloacae, 19 serratia marcescens, 2 serratia liquefaciens, 8 prot ...	1980	6999020
biochemical characteristics and identification of enterobacteriaceae isolated from meats.	the isolation and identification of 2,220 enterobacteriaceae from meats indicated that escherichia coli biotype i, enterobacter agglomerans, and serratia liquefaciens were the principal types to be differentiated in meats. citrobacter freundii, klebsiella pneumoniae, enterobacter cloacae, and enterobacter hafniae were also commonly identified. identification of isolates by the encise ii (roche diagnostics inc., nutley, n.j.) and minitek (bbl microbiology systems, cockeysville, md.) coding system ...	1981	7013705
studies on virulence of shigella flexneri. protective effect of outer membrane proteins.	groups of mice were immunized intraperitoneally with proteins isolated from outer membrane of virulent strain of shigella flexneri 3a and its avirulent variant. all the animals were found to survive challenge with ld100 of virulent sh. flexneri 3a. similar protective effect against challenge with sh. flexneri 3a gave also immunization of mice with outer membrane proteins isolated from sh. flexneri of other serotypes (2a, 4a, 6, and x) and from escherichia coli, hafnia alvei and shigella sonnei.	1981	7025793
a case with severe diarrhoea and strongyloides stercoralis infection.	a case with severe diarrhoea and strongyloides stercoralis infection is described. further examination showed that the patient also had abnormal colonization of the duodenum with hafnia alvei and that this disappeared when the strongyloides infection was treated with mebendazole. symptoms such as abdominal pain, diarrhoea, "skin rash" and malabsorption in association with blood eosinophilia should arouse suspicion of strongyloidiasis.	1981	7234512
aerobic bacterial oral flora of garter snakes: development of normal flora and pathogenic potential for snakes and humans.	garter snakes that are used for scientific laboratory studies or kept as exotic pets often become ill and die early in captivity. they may also act as reservoirs of potential human pathogens or transmit infection to man. a total of 126 strains of aerobic and facultative bacteria, most potential human and snake pathogens, were isolated from 82 garter snake oropharyngeal cultures. coagulase-negative staphylococcus species were the most common species isolated. acinetobacter calcoaceticus var. anit ...	1981	7240404
activity of a new cephalosporin antibiotic, ro 13-9904 against dense populations of selected enterobacteria.	ro 13-9904 is a new cephalosporin antibiotic with a wide spectrum of activity similar to that of cefotaxime and some other newer beta-lactam compounds. in the present study, the response to ro 13-9904 of 13 strains of enterobacteria, selected on the basis of differential patterns of susceptibility to beta-lactam agents, was investigated. the new cephalosporin displayed high intrinsic activity against most of the enterobacteria tested, but a group of klebsiella and hafnia alvei strains was identi ...	1981	7247361
report of the 16th quadrennial congress of the international council of nurses held in tokyo city, japan from 30th may to 3rd june 1977.		1981	243752
fifth stenhous-williams memorial lecture. oxygen and the obligate anaerobe.		1981	181358
comparison of two methods for same-day identification of enterobacteriaceae.	two commercial methods, api 20e (as modified for same-day enterobacterial identification) and micro-id, were evaluated for ability to provide useful same-day information of 368 clinically isolated enterobacteriaceae. organisms included escherichia coli (54), shigella (7), edwardsiella tarda (1), salmonella enteritidis (10), citrobacter (30), klebsiella (55), enterobacter (68), hafnia alvei (2), serratia (33), proteus (64), morganella morganii (24), providencia (18), and yersinia enterocolitica ( ...	1982	7051807
comparison of three methods for identification of enterobacteriaceae.	this study compares the ability of three commercial overnight methods, api 20e, minitek and enteric-tek, to accurately and completely identify 368 clinically isolated enterobacteriaceae without supplemental tests. organisms included escherichia coli (54 strains), shigella spp. (7), edwardsiella tarda (1), salmonella enteritidis (10), citrobacter spp. (30), klebsiella spp. (55), enterobacter spp. (68), hafnia alvei (2), serratia spp. (33), proteus spp. (64), morganella morganii (24), providencia ...	1982	6756905
rhodanese activity: a simple and reliable taxonomic tool for gram-negative bacteria.	the thiosulphate: cyanide sulphurtransferase (rhodanese) test of vandenbergh, bawdon and berk (1979) has been simplified and 2469 strains from a wide variety of sources representing different biochemical, serological or phage-pattern entities were tested. the percentages of rhondanese-producing strains were: escherichia coli 98%, shigella flexneri serovars 1-5%, x and y 0%, other shigellae 73-100%, yersinia spp. 0%, salmonella subgenera i-iv 0%, citrobacter freundi 16%, klebsiella 37%, enterobac ...	1982	6958873
retardation of wool growth in merino sheep caused by bacteria.	a condition evidenced by retarded growth of wool with alteration of the yolk into a yellow, sticky, wax-like substance was investigated. the condition was associated with hyperaemia and cellular infiltration into the dermis in the affected areas. three bacterial species, viz. enterobacter aerogenes, e. agglomerans and hafnia alvei, which could grow on the water-extractable component of wool-yolk, were incriminated as the cause of the condition.	1983	6676689
evaluation of the updated ms-2 bacterial identification system in comparison with the api 20e system.	the recently updated ms-2 bacterial identification system software (abbott laboratories, diagnostic division, irving, tex.) was compared with the original ms-2 bacterial identification software and the api 20e, using 968 strains of enterobacteriaceae. the updated ms-2 software correctly identified 94.4% of the isolates tested. api 20e and the original ms-2 software correctly identified 91 and 85.3% of the strains, respectively. ms-2 responses were considered to be equivocal (needing additional t ...	1983	6350342
[influence of hemolytic plasmids on the development of the "killing" effect in various species of enterobacteria].	experiments on isogenic strains of different enterobacterial species, both carrying hemolytic plasmids and free from them, have demonstrated that their "killing" effect on mice is linked with the yield of hemolysin and does not depend on the origin of hly plasmids or the chemotype of bacteria. plasmid p ie 567 coding the synthesis of alpha-hemolysin has been shown to be capable of expression in enterobacter aerogenes, hafnia alvei and salmonella typhimurium strains, but e. aerogenes 3/43, h. alv ...	1983	6356727
[in vitro bacteriostatic activity of cefmenoxime (sce 1365), cefotaxime and moxalactam].	the in vitro activity of cefmenoxime (sce 1365), a new cephalosporin derivate was compared with two other "third generation" cephalosporins: cefotaxime and moxalactam. cefmenoxime as cefotaxime and moxalactam were very active against 305 cephalosporinase-producing and cephalosporinase-non-producing enterobacteriaceae. cefmenoxime was the most active against serratia marcescens, citrobacter freundii, morganella morganii, salmonella, shigella and yersinia enterocolitica with a mean mic at least tw ...	1983	6312395
biosynthesis of bacterial glycogen: characterization of adenosine diphosphate glucose synthetases from enterobacter hafniae and aeromonas hydrophila.	enterobacter hafniae and aeromonas hydrophila adpglucose synthetases were purified approximately 39- and 61-fold, respectively, over the crude extract. both enzymes were heat stable at 60 degrees c in the presence of inorganic phosphate. the molecular weights of both enzymes were approximately 200,000 which are similar to other enteric adpglucose synthetases studied. based on kinetic results obtained from the partially purified enzymes, the e. hafniae enzyme is activated twofold by phospho-enolp ...	1984	6331331
effect of chlorination on antibiotic resistance profiles of sewage-related bacteria.	a total of 1,900 lactose-fermenting bacteria were isolated from raw sewage influent and chlorinated sewage effluent from a sewage treatment plant, as well as from chlorinated and neutralized dilute sewage, before and after a 24-h regrowth period in the laboratory. of these isolates, 84% were resistant to one or more antibiotics. chlorination of influent resulted in an increase in the proportion of bacteria resistant to ampicillin and cephalothin, the increase being most marked after regrowth occ ...	1984	6476832
[study of lincomycin concentrations in hepatocystic duct].	penetration of lincomycin (lcm) in choledochal and cholecystic bile as well as in the gallbladder tissue and liver tissue was investigated together with bacteria detectable in the bile in order to evaluate basically usefulness of this antibiotic in the treatment of infections of the hepatocystic duct. intravenous drip infusion of lcm 1.5 g (in 500 ml of 5% glucose solution) over 1.5--2 hours resulted in mean drug concentrations of 33.9 and 10.1 micrograms/ml in serum at 2 and 4 hours post start ...	1984	6502929
kinetic mechanism and location of rate-determining steps for aspartase from hafnia alvei.	coupled spectrophotometric assays that monitor the formation of fumarate and ammonia in the direction of aspartate deamination and aspartate in the direction of fumarate amination were used to collect initial velocity data for the aspartase reaction. data are consistent with rapid equilibrium ordered addition of mg2+ prior to aspartate but completely random release of mg2+, nh4+, or fumarate. in addition to mg2+, mn2+ can also be used as a divalent metal with vmax 80% and a kaspartate 3.5-fold l ...	1984	6509019
a new partition thin-layer chromatographic method for steroid separations.	tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ...	1985	3835732
[minimally transformed 3t3 cell lines as an object for research on the mechanisms of proliferation].	the present review gives a detailed description of mouse cell lines 3t3 (swiss 3t3, balb/c 3t3, and nih 3t3) including their establishment, evolution, growth properties and formation of the specific monolayer with low saturation density. questionable views of the 3t3 cell origin are discussed. the main features of these continuous cell lines are shown, such as a high requirement for serum growth factors, substrate dependence, inhibition of cell division in dense monolayer and absence of oncogeni ...	1985	3885522
characteristics of insulin binding to h35 hepatoma cells.	well differentiated hepatoma cells in culture exhibit insulin binding and insulin effects. we have studied insulin binding in control and in h35 hepatoma cells down-regulated with insulin. h35 cells were grown in monolayers in alpha mem. insulin binding was measured with a14 mono 125i labelled insulin 72 h after seeding. binding was time, temperature and ph-dependent. receptor down-regulation was studied by exposing cells to increasing concentrations of unlabelled insulin. monolayers preincubate ...	1985	3907721
[inducible cephalosporinase: study of enterobacter hafniae].		1985	3913617
a role for inhibin in the control of follicle-stimulating hormone secretion in male rats.	we examined the relationship of testosterone (t) and porcine follicular fluid (pff) in the negative feedback control of fsh and lh secretion in adult male rats. either at the time of castration (acute) or at least 30 days after castration (chronic), we implanted t-filled silastic capsules, which were 2 mm, 10 mm, or 30 mm long; empty capsules (30 mm) served as controls. seven days later, we injected either 0.15 ml of pff or saline (i.v.), decapitated the rats 6 hours later, and collected trunk b ...	1985	3919236
koserella trabulsii, a new genus and species of enterobacteriaceae formerly known as enteric group 45.	the name koserella trabulsii is proposed for a group of enterobacteriaceae formerly called enteric group 45. this group consists of 12 strains that were originally identified as atypical hafnia alvei. k. trabulsii strains were negative for indole production, voges-proskauer, h2s production, urea hydrolysis, phenylalanine deaminase, and acid production from glycerol, lactose, sucrose, and d-sorbitol; they were positive for methyl red, citrate (simmons), lysine and ornithine decarboxylases, argini ...	1985	3968202
[persistent chlamydia infection in cell culture].		1985	3993192
diurnal variations in serum lithium and renal lithium clearance in rats given lithium as a single small dose or as multiple high doses.	clearance determinations were carried out in three groups of rats: a high lithium group given food to which 70 mmol/kg of lithium were added for 4-6 weeks leading to a mean serum lithium concentration of 0.85 mmol/l, a low lithium group given food to which 15 mmol/kg of lithium were added for two days before the clearance period leading to a mean serum lithium concentration of 0.22 mmol/l, and a group given the same food without lithium. the sodium and potassium contents of the food were kept hi ...	1985	4061097
massive callus formation after distal femoral fracture in association with trisomy 2.	massive callus formation in a distal femoral metaphyseal fracture of a trisomy-2 patient is reported. the child was neurologically severely handicapped. the possible pathomechanics of the callus formation is discussed.	1985	4062522
[effects of 5 derivatives of 3-azabicyclo [3,3,1]nonanes on isolated guinea pig ileum myenteric plexus-longitudinal muscle].		1985	3020868
peroxisomal oxidases and suggestions for the mechanism of action of insulin and other hormones.		1985	2863924
hypotensive effects after topical and intra-arterial administration of alpha-adrenergic agonists in isolated perfused rabbit eyes.	the influence of nonselective and selective alpha-adrenergic agonists on intraocular pressure (iop) was studied in isolated arterially perfused rabbit eyes. drugs were given intra-arterially or topically. with either route of administration, no initial hypertensive phase was observed after l-epinephrine, l-norepinephrine, l-phenylephrine (alpha 1), or methoxamine (alpha 1), but there was an immediate ocular hypotensive effect. the alpha 2-agonist b-ht 920 caused a nonsignificant 1 mm hg fall in ...	1985	2866480
regulation of the glucose phosphotransferase system in brochothrix thermosphacta by membrane energization.	uptake of 2-deoxyglucose, alpha-methylglucopyranoside, and glucose into intact cells of brochothrix thermosphacta (formerly microbacterium thermosphactum, atcc 11509) was stimulated by kcn or cccp. the glucose analogs were recovered almost totally as the sugar phosphates. membrane vesicles were isolated from protoplasts and shown to be right side out by freeze fracturing and by using atpase as a marker for the cytoplasmic membrane surface. uptake of glucose into vesicles was dependent on the pre ...	1985	2995314
anti-ischemic actions of a new thromboxane receptor antagonist during acute myocardial ischemia in cats.	thromboxane a2 (txa2) production increases significantly during acute myocardial ischemia. since txa2 induces platelet aggregation, coronary vasoconstriction, and has a direct cytolytic effect, thromboxane receptor antagonism would be expected to be beneficial in acute myocardial ischemia. thirty minutes after ligation of the left anterior descending coronary artery (lad) in anesthetized cats, the txa2 receptor antagonist bm-13,177 or its vehicle was given as a bolus injection at 20 mg/kg, follo ...	1985	3000159
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
acetyl-homocysteine-thiolactone-induced increase of superoxide dismutase counteracts the effect of subdiabetogenic doses of streptozocin.	acetyl-homocysteine-thiolactone (cyt) is an organic thio compound that exerts free radical scavenger activity and increases superoxide dismutase (sod) activity. administration of 32 mg cyt/kg body wt/day/30 days in rats increased sod activity in erythrocytes by 126%, and in pancreatic islets by 202%. treatment affected only the cu-zn fraction of the enzyme. transmission electron microscope observations showed that the damage to the pancreatic beta cells induced by single or multiple subdiabetoge ...	1986	2937674
[anopheles breeding in the municipal reservoir of the city of ustinov].		1986	2945087
[a test for the identification of hafnia alvei bacteria by the color reaction with a complex of 5-nitro-8-hydroxyquinoline + iron ions].		1986	2429042
antimicrobial activity of imipenem against 1386 clinical isolates.	1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...	1986	3468319
identification of the stable free radical tyrosine residue in ribonucleotide reductase.	the small subunit of iron-dependent ribonucleotide reductases contains a stable organic free radical, which is essential for enzyme activity and which is localized to a tyrosine residue. tyrosine-122 in the b2 subunit of escherichia coli ribonucleotide reductase has been changed into a phenylalanine. the mutation was introduced with oligonucleotide-directed mutagenesis in an m13 recombinant and verified by dna sequencing. purified native and mutant b2 protein were found to have the same size, ir ...	1986	3019680
identification of the stable free radical tyrosine residue in ribonucleotide reductase.	the small subunit of iron-dependent ribonucleotide reductases contains a stable organic free radical, which is essential for enzyme activity and which is localized to a tyrosine residue. tyrosine-122 in the b2 subunit of escherichia coli ribonucleotide reductase has been changed into a phenylalanine. the mutation was introduced with oligonucleotide-directed mutagenesis in an m13 recombinant and verified by dna sequencing. purified native and mutant b2 protein were found to have the same size, ir ...	1986	3019680
in vitro drug susceptibility and doses of metronidazole required for cure in cases of refractory vaginal trichomoniasis.	there are currently no laboratory or clinical guidelines for the identification and treatment of disease caused by metronidazole-resistant strains of trichomonas vaginalis. fifty-three isolates of t. vaginalis from cases of refractory vaginitis in the united states (26 states) and canada were tested for aerobic and anaerobic metronidazole susceptibility, and after various dosages of metronidazole, the therapeutic outcomes were evaluated for 31 of these cases. the mean aerobic metronidazole susce ...	1986	3486237
ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria.	the iron uptake test of szabó and vandra has been modified and used for the differentiation of gram-negative bacteria. nutrient agar containing 20 g per litre of ferric ammonium citrate was distributed into narrow tubes and solidified so as to form butts and slants. considering the localization of the rusty-brown coloration produced after seeding and incubation, 2367 strains were classified into four groups. (1) unchanged medium: escherichia coli, shigella spp., yersinia spp., hafnia alvei and m ...	1986	3529797
heterologous protection against rotavirus-induced disease in gnotobiotic piglets.	administration per os of 2 x 10(6) fluorescent cell-forming units of a human serotype 3 rotavirus (rv-3) protected all of nine gnotobiotic piglets against severe diarrheal disease when they were challenged 10 to 14 days later with 8 x 10(3) fluorescent cell-forming units of virulent wild-type porcine rotavirus (at/76). the porcine virus was similar antigenically to porcine prototype strain osu, previously described as antigenically distinct from all four recognized human serotypes. administratio ...	1986	3023436
modulation of complement fixation and the phlogistic capacity of group a, b, and d streptococci by human lysozyme acting on their cell walls.	streptococci and streptococcal cell wall fragments induce arthritis in rats, with the severity and duration depending on the capacity of the cells or cell fragments to resist degradation by tissue enzymes. their phlogogenic effects are apparently related to their ability to activate the alternate complement pathway (acp). the in vitro activation of the acp by lysozyme-treated cells and cell walls of group a, b, and d streptococci suggests that both rat and human lysozyme can modulate this activi ...	1986	3086232
in vitro activity of aztreonam against gram negative bacteria from clinical specimens and its comparison with other commonly used antibiotics.	a total of 755 gram negative bacteria isolated from clinical specimens were tested against aztreonam by the disc agar diffusion test. the strains of bacteria used in this study consisted of escherichia coli (314), enterobacter aerogenes (30), e. agglomerans (7), e. cloacae, (39), citrobacter diversus (9), c. freundii (13), hafnia alvei (3), acinetobacter calcoaceticus (10), klebsiella oxytoca (6), k. ozaenae (5), k. pneumoniae (107), morganella morganii (3), moraxella sp. (10), pasteurella multo ...	1986	3088346
galactosyltransferase: multiple forms in serum of normal and hepatoma mc-29 bearing chickens and from liver and hepatoma microsomal and plasma membrane preparations.	the multiple forms of galactosyltransferase in chicken serum and in microsomal and plasma membrane preparations from liver and viral induced hepatoma mc-29 have been studied by isoelectric focussing. an elevation of the hepatoma plasma membrane enzyme activity was described and in the pattern of the multiple forms of the enzyme two forms were found (pi-5.34 and 8.22) which were similar to those described in the serum of hepatoma bearing chickens (pi-5.36 and 8.24). a conclusion is drawn that the ...	1986	3102037
acute circulatory effects of doxorubicin in the conscious dog.	experimental work on the mechanisms of acute doxorubicin-induced cardiotoxicity may contribute to a better understanding of the clinical problem of cardiac failure after treatment with anthracycline derivatives. we studied aortic pressure and heart rate continuously for 1 h following a bolus injection of doxorubicin (1 mg/kg) in 7 dogs. in contrast with previous studies in intact animals, no anesthesia was used in order to eliminate possible interactions of doxorubicin with other drugs. one minu ...	1986	3153244
suppression of plasma aldosterone during stimulation of cardiopulmonary mechanoreceptors in man.		1986	3553842
an improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova.	we have developed an improved method for analyzing human sperm chromosome, using zona-free hamster ova. our main improvements of methodology are as follows: (1) fertilization rate of hamster oocytes by human spermatozoa was markedly raised by successive treatments of the spermatozoa with 5-15 microm ionophore a23187 solutions and a capacitation medium (bww medium) containing 3.5% hsa. the hsa most effective in inducing capacitation was selected from several kinds of hsa products commercially ava ...	1986	3717161
inhibition of yersinia enterocolitica serotype o3 by natural microflora of pork.	yersinia enterocolitica serotype o3 did not grow but did survive in inoculated raw ground pork kept at 6 and 25 degrees c. the antagonistic effect of microbial flora, especially hafnia alvei and environmental yersinia organisms, on the growth of y. enterocolitica serotype o3 in raw ground pork was evident. these results were supported by evidence of the inhibition of growth of y. enterocolitica serotype o3 by enterobacteriaceae, especially h. alvei and environmental yersinia organisms, in mixed ...	1986	3729395
analysis of factors involved in determining urinary bladder voiding cycle in urethan-anesthetized rats.	the mechanism(s) involved in determining the voiding cycle of the rat urinary bladder have been investigated in urethan-anesthetized animals. fluid emission is almost confined to that phase of the voiding cycle which is characterized by the presence of a series of high-frequency oscillations in intraluminal pressure (iphfo). during this phase the mean urethral flow rate reached a maximum and fluid was expelled in a stream-like fashion. the index obtained by multiplying the amplitude of iphfo by ...	1986	3740305
effects of dorsal column transection in the upper cervical segments on visually guided forelimb movements.	complete transection of the dorsal column in c2 in cat gave severe defects in forelimb target-reaching and food-taking tested with retrieval of food from a cylinder. among the symptoms were marked dysmetria in all directions and dyscoordination of movements in different joints, with only slow recovery over weeks and months. it is postulated that normal visual guidance of forelimb movements to a stationary target depends on somatosensory information to the brain via the dorsal column.	1986	3748476
[current methods of chromosome studies].		1986	3769837
bacterial sources of putrescine and cadaverine in chill stored vacuum-packaged beef.	of the meat strains of streptobacteria, leuconostocs, enterobacteriaceae and brochothrix thermosphacta tested, only hafnia alvei and serratia liquefaciens showed diamine-producing potential during growth in pure culture on beef stored in vacuum packs at 1 degree c. both organisms produced cadaverine at concentrations similar to those reported previously in naturally contaminated beef stored under the same conditions. putrescine concentrations produced by the two organisms, however, were an order ...	1986	3771410
[preparation of the isolated retina of rana nigromaculata and determination of the photosensitivity of rhodopsin in situ].		1986	3775398
cytopathic liver injury in acute delta virus hepatitis.	hepatitis delta-virus (the delta-agent) is now recognized as a cause of acute hepatitis, fulminant hepatitis, chronic hepatitis, and cirrhosis in hepatitis b surface antigen-positive hosts. this report describes the clinical course and liver biopsy histopathology of acute delta-hepatitis in a young american woman with presumed chronic hepatitis b infection. the liver biopsy specimen features included severe cytotoxic and cytopathic hepatocellular damage, small droplet vacuolar liver cell degener ...	1987	3557018
acoustic tumor in a young adult: documented growth rate.	the diagnosis of acoustic neuroma is being made more easily with the advent of late-generation, high-resolution computed tomography. it is known that acoustic tumor growth in the elderly is often slow. on the other hand, it is believed that an acoustic tumor can grow very rapidly in the young. a case of acoustic neuroma in a young man with computed tomographic evidence of extremely rapid growth over a period of fifteen months is presented.	1987	3591916
enterobacteriaceae, coliforms, faecal coliforms and salmonellas in raw ewes' milk.	thirty-nine samples of freshly drawn ewes' milk collected at 13 farms, and 120 samples of raw ewes' milk collected on arrival at a dairy were examined. farm samples had geometric mean counts of 4.4 x 10(2) enterobacteriaceae/ml, 3.9 x 10(2) coliforms/ml and 2.0 x 10(2) faecal coliforms/ml, whereas the respective mean counts were 6.2 x 10(3)/ml, 5.4 x 10(3)/ml and 1.3 x 10(3)/ml for dairy samples. salmonellas were not detected by enrichment procedures in any of the 159 samples examined. escherich ...	1987	3597207
albendazole therapy in alveolar hydatid disease: a report of favorable results in two patients after short-term therapy.	albendazole was administered preoperatively to two patients with active alveolar hydatid disease for 58 and 84 days. vesicles of larval echinococcus multilocularis obtained from surgical tissues were inoculated into red-backed voles for in vivo testing viability. no proliferation of the larval cestode had occurred when the animals were dissected three months post-inoculation. these findings suggest that short-term therapy with albendazole was effective in killing the larval cestode in these two ...	1987	3605498
[studies on the metabolic fate of 14c-rokitamycin. i. absorption, distribution, metabolism and excretion in rats].	blood concentrations of 14c-rokitamycin (14c-tms-19-q) reached their peaks at 1 hour after a single oral (200 mg/kg) administration to male and female rats, and they were 28.0 +/- 0.8 and 24.9 +/- 2.0 micrograms/ml, respectively. no significant differences were observed between male and female in auc values or maximum blood concentrations. the distribution of tms-19-q was good, and concentrations of 14c were high in liver, kidney, spleen, pancreas, adrenal, pituitary gland, thyroid, trachea, exo ...	1987	3613074
[ideal experimental microsurgical methods in vasovasostomy and vasoepididymostomy].	the vasovasostomy with the double-layered end-to-end anastomosis by silber is a microsurgical ideal method. we think we found experimentally an ideal method for the vasoepididymostomy with the open end-to-side anastomosis and direct spermato-tight microsurgical suture. specialties of the species rat and dog have to be observed. restrictions of the anastomotic region lead to infertility.	1987	3617849
the application of 1h/13c inversely correlated nmr spectroscopy to the determination of acylation and glycosylation sites in the o-specific polysaccharide from hafnia alvei 1187.	an inversely correlated 1h/13c nmr spectrum defined the amino sugars acylated by acetyl or 3-hydroxybutyryl groups and revealed partial sequences and glycosylation sites in a tetrasaccharide repeating unit of the title polysaccharide, (----2dglc alpha 1----3dglcnacyl alpha 1----4dgalnac alpha 1----3dgalnac beta 1----)n, where acyl = 3-hydroxybutyryl.	1987	3619933
night-day differences in the ultradian rhythmicity of plasma renin activity.	during night-sleep, plasma renin activity displays periodic oscillations which are closely related to the alternation of rem-nrem sleep. to see whether this nocturnal rhythm persisted during the day-time, plasma renin activity was measured every 10 min for 24 hours in 4 human volunteers and in 4 others over a 10-h day-time period. to avoid the influence of repeated food intake which stimulates renin release, the subjects were on continuous enteral nutrition. spectral analyses of the data reveale ...	1987	3108599
lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats.	lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ...	1987	3122678