Publications

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formation of complexes between dna isolated from tobacco crown gall tumours and rna complementary to agrobacterium tumefaciens dna. 19676064642
[action of capsicine on the multiplication and virulence of agrobacterium tumefaciens]. 19676077893
metabolism of amino acids in agrobacterium tumefaciens. 3. uptake of l-pro- line. 19676082570
[ribonucleic acids in crown-gall agrobacterium tumefaciens (smith and town) conn. bacteria exposed to sodium borate]. 19684878338
murine toxicit of agrobacterium tumefaciens.eleven strains of the crown gall organism, agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. five other species had some lethal strains. the lethal effect of a. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-killed cultures were lethal. the murine toxin disappeared when a. tumefaciens was grown at 36 c and reapp ...19685643064
methylation of the cellular lipid of methionine-requiring agrobacterium tumefaciens.mutants of agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of n-methyl-n'-nitro-n-nitrosoguanidine. in addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumarate. when ((14)c-methyl)-methionine was added to strain wm-11 growing in a prescribed basal medium, incorporation of (14)c into the cellular "residue" fraction and polar "n-methylate ...19685669890
[bacteriophages of agrobacterium tumefaciens (smith and town.) conn. i. isolation and characteristics]. 19685672117
[bacteriophages of agrobacterium tumefaciens. ii. lysogenic strain b6m in the tissues of pisum sativum l, and the effect of glycine on phage production]. 19685674588
ultraviolet repair capabilities of agrobacterium tumefaciens. 19685681510
activation of the tumor-inducing ability of agrobacterium tumefaciens (smith and townsend) conn. by means of capsicin. 19685709363
hexopyranoside: cytochrome c oxidoreductase from agrobacterium tumefaciens. 19685726844
[effect of maleic hydrazide on the agrobacterium tumefaciens. i. study of the action of maleic hydrazide on the growth and synthesis of beta-indolacetic acid]. 19685731452
[influence of maleic hydrazines on the agrobacterium tumefaciens. ii. formation of tumors due to the action of maleic hydrazine on the bacteria and precursors of beta-indolacetic acid]. 19685731453
cytochrome c552 in agrobacterium tumefaciens. 19684302139
[submerse bacterial plant tumor on roots].spontaneous infections seem to indicate that not only the crown of herbeous plants is disposed to generate tumors. under substrate conditions such as those normally found at the crown, infections caused by agrobacterium tumefaciens will yield tumors in the entire area of the roots.roots of helianthus annuus and solanum lycopersicum grown in nutritive medium are disposed to tumor when given appropriate conditions of culture and inoculation. tissue culture, the most widely applied method for the a ...196824522818
conversion of glucose-1-phosphate to 3-keto-glucose-1-phosphate by cells of agrobacterium tumefaciens.incubation of resting cells of agrobacterium tumefaciens with glucose-1-phosphate resulted in the accumulation of a new sugar phosphate in the suspending medium. approximately 80% of the glucose-1-phosphate consumed was converted to the new compound, which was identified as alpha-d-ribo-hexopyranosyl-3-ulose-1-phosphate (3-ketoglucose-1-phosphate). both utilization of glucose-1-phosphate and accumulation of 3-ketoglucose-1-phosphate were inhibited by 2,4-dinitrophenol, polymyxin, and d-glucose, ...19694304223
hexuronic acid dehydrogenase of agrobacterium tumefaciens.growth of agrobacterium tumefaciens on d-glucuronic acid (glcua) or d-galacturonic acid (galua) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (nad) oxidoreductase]. the dehydrogenase, which irreversibly converts glcua or galua to the corresponding hexaric acid with the concomitant reduction of nad, but not of nicotinamide adenine dinucleotide phosphate was purified 60-fold by mncl(2) treatment, (nh(4))(2)so(4) fractionation, chromatogr ...19694313130
bacterial attachment to a specific wound site as an essential stage in tumor initiation by agrobacterium tumefaciens.the number of tumors initiated by agrobacterium tumefaciens strain b6 on primary pinto bean leaves was decreased when cells of an avirulent strain (iibnv6) were included in the inoculum. with sufficient b6 cells to initiate ca. 50% of the maximal number of tumors per leaf, inhibition was detected at a 1:1 ratio of b6 to iibnv6 cells and increased linearly with the logarithm of the number of iibnv6. varying the number of b6 in the presence of a constant number of iibnv6 or varying the number of b ...19695773014
bacteriophage release in a lysogenic strain of agrobacterium tumefaciens.bacteriophage release in a lysogenic strain of agrobacterium tumefaciens v-1 is temperature-sensitive. at 25 c and 30 c, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 c, although bacterial growth was not inhibited, phage release was suppressed. phage synthesis was induced by heat shock, 42 c for 30 min, ultraviolet irradiation, and mitomycin c. induction by ultraviolet light was unusual-an immediate rise in phage titer followed irradiation. a large increase occur ...19695774138
methylation of fatty acids in a methionine-dependent agrobacterium tumefaciens controlled with exogenous methionine. 19695811212
agrobacterium tumefaciens rna in non-tumorous tomato cells. 19695353894
active transport of uridine diphosphate glucose in agrobacterium tumefaciens. 19695366997
[studies on the production of a cytokinic substance by agrobacterium tumefaciens (smith and town) conn]. 19695373660
growth requirements and infectivity of auxotrophic adenine-dependent mutants of agrobacterium tumefaciens. 19695374246
[interspecific transformations of agrobacterium tumefaciens and rhizobium leguminosarum]. 19695384628
[electron microscopy of tumor tissue in phaseolus vulgaris by agrobacterium tumefaciens]. 19695386306
anomaly in the neutralization kinetics of phages of agrobacterium radiobacter. 19695392738
[antagonistic relationship between actinomycetes and agrobacterium tumefaciens (smith and townsend) conn. i]. 19695398252
the nature, intergeneric distribution and biosynthesis of isoprenoid quinones and phenols in gram-negative bacteria.1. twenty-two aerobically grown gram-negative bacteria were analysed for demethylmenaquinones, menaquinones, 2-polyprenylphenols, 6-methoxy-2-polyprenylphenols and ubiquinones. 2. all the eight enterobacteria and both the two facultative organisms (aeromonas punctata and aeromonas hydrophila) examined contain all the compounds listed above. the principal homologues are octaprenyl; in addition lower (down to tri- or tetra-prenyl for the 2-polyprenylphenols) and sometimes higher homologues are als ...19694886765
suspected mutant of agrobacterium tumefaciens with an altered dna. 19694979962
agrobacterium tumefaciens cross-reacting antigens in sterile crown-gall tumors. 196911946999
physiological comparisons of pith callus with crown-gall and genetic tumors of nicotiana glauca, n. langsdorffii, and n. glauca-langsdorffii grown in vitro. i. tumor induction and proliferation.agrobacterium tumefaciens b-6 and t-37 strains, inoculated into nicotiana glauca, n. langsdorffii, and their interspecific hybrid, which forms genetic (spontaneous) tumors as well, initiate amorphous tumors from the b-6 strain and organoid tumors (aberrant roots, stems, and buds) from the t-37 strain. in the hybrid, the critical point was to induce crown gall tumors at the site of wounding and not spontaneous genetic tumors. to succeed, this inoculation had to be made at a very early (5-6 leaf s ...196916657159
physiological comparisons of pith callus with crown-gall and genetic tumors of nicotiana glauca, n. langsdorffii, and n. glauca-langsdorffii grown in vitro. ii. nutritional physiology.explants of genetic tumors, tumors initiated by agrobacterium tumefaciens strains b-6 and t-37, and excised pith plugs from nicotiana glauca, n. langsdorffii, and n. glauca-langsdorffii were cultured on murashige and skoog medium. all cultures, pith callus and tumors with the exception of n. langsdorffii pith grew on this medium. addition of glutamine to the medium resulted in highly organoid growth in n. langsdorffii pith. in order to have material comparable to other pith cultures, n. langsdor ...196916657160
studies on auxin protectors. vii. association of auxin protectors with crown gall development in sunflower stems.mature sunflower internodes contain only very low concentrations of auxin protectors. wounding such internodes results in a temporary increase of protector substances. inoculating the wounds with a virulent strain of agrobacterium tumefaciens conn results in a dramatic rise in protector substances, particularly the very high molecular weight protector "a", which continues as the tumors develop. the levels attained in tumor tissue are comparable to those normally encountered in meristematic tissu ...196916657184
transmission of an agrobacterium tumefaciens phage by pristionchus iheriteiri.pristionchus lheriteiri (maupas) paramonov, a saprozoic nematode, served as a carrier of an unnamed phage of agrobacterium tumefaciens (smith and townsend) conn. viable phage particles passed through the nematode, caused lysis and formed typical plaques on agar plates seeded to a. tumefaciens. phage retention by carrier nematodes was extended several hr by restricting food intake. female nematodes accumulated phage in greater quantities and more rapidly than male nematodes.196919325671
saprozoic nematodes as carriers and disseminators of plant pathogenic bacteria.the plant pathogenic bacteria agrobacterium tumefaciens (smith and townsend) conn. (strain 5-14 deep), erwinia amylovora (burill) winslow et al., e. carotovora (jones) holland and pseudornonas phaseolicola (burk.) dows. (icpb-pm3) and the red-pigmented non-pathogen serratia marcescens bizio were hosts for the saprozoic nematode pristionchus iheritieri (maupas, 1919) paramonov. viable bacteria survived passage through the nematode and produced typical colonies on nutrient agar plates. female nema ...196919325679
lysopine and octopine promote crown-gall tumor growth in vivo.growth of tumors induced on primary leaves of bean plants by agrobacterium tumefaciens was increased by the addition of lysopine and octopine. a detectable response was observed when as little as 1 microgram of these compounds was added per leaf, and the mean volume of the tumors was increased two- to threefold when greater amounts were applied. the specificity of the response and the unique association of these compounds with the tumors suggest that endogenous lysopine and octopine contribute t ...197017833501
gas-liquid chromatographic isolation of cytokinins from natural sources: 6-(3-methyl-2-butenylamino) purine from agrobacterium tumefaciens.gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. with one exception, all of the naturally occurring cytokinins are separated.ethyl acetate extraction of yeast transfer rna hydrolysates and of culture filtrates of agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography. this procedure permitted the detection of 6-(3-methyl-2-butenyla ...197016657339
bioassay and attributes of a growth factor associated with crown gall tumors.an improved bioassay is described for a factor that promotes tumor growth which was first obtained from extracts of pinto bean leaves with crown gall tumors. sixteen primary pinto bean leaves per sample are inoculated with sufficient agrobacterium tumefaciens to initiate about 5 to 10 tumors per leaf and treated with tumor growth factor at day 3 after inoculation. the diameters of 30 to 48 round tumors (no more than 3 randomly selected per leaf) are measured per test sample at day 6. mean tumor ...197016657534
enhancement of agrobacterium tumefaciens infectivity by chlorpromazine hydrochloride.the tumor-initiating ability of agrobacterium tumefaciens was enhanced two- to threefold after a preinoculation exposure to the phenothiazine derivative chlorpromazine hydrochloride. the mean number of tumors initiated per viable bacterium on pinto bean leaves was greatest after a 1-hr exposure to 15 mug of the drug per ml. chlorpromazine had no significant effect on tumor initiation when applied to previously inoculated leaves. chlorpromazine at 10 mug/ml was bacteriostatic; concentrations of 2 ...197016557863
physicochemical properties of agrobacterium tumefaciens phage lv-1 and its dna. 19705411193
[observations on the presence of phages in wounds infected with different strains of agrobacterium tumefaciens (smith and town) conn]. 19705422870
active transport of glucose-1-phosphate in agrobacterium tumefaciens.the presence of an active transport system for glucose-1-phosphate in agrobacterium tumefaciens was demonstrated from the following observations. (i) the bacterium could grow on a medium containing glucose-1-phosphate as carbon source; (ii) the entry of glucose-1-phosphate into the resting cells occurred against concentration gradient obeying michaelis-menten kinetics; and (iii) the entry reaction was energy-dependent. the transport system for glucose-1-phosphate was formed inducibly by growing ...19705438042
alpha-3-ketoglucosidase of agrobacterium tumefaciens.a 3-ketosucrose-degrading enzyme was purified 80-fold from the sonic extracts of agrobacterium tumefaciens iam 1525 grown on a sucrose-containing medium. the enzyme catalyzes hydrolysis of alpha-3-ketoglucosides such as 3-ketosucrose, 3-ketotrehalose, 3-ketomaltose, and 3-ketoglucose-1-phosphate but not of beta-3-ketoglucosides, beta-3-ketogalactosides, and other glycosides such as sucrose, trehalose, maltose, glucose-1-phosphate, cellobiose, lactose, or raffinose. from the strict substrate spec ...19705438043
biosynthesis of bacterial glycogen. 8. activation and inhibition of the adenosine diphosphoglucose pyrophosphorylase of rhodopseudomonas capsulata and of agrobacterium tumefaciens. 19705460185
[intervention in the inoculation wound of bacteria belonging to various strains of agrobacterium tumefaciens (smith and town) conn]. 19705478546
specificity patterns of agrobacterium tumefaciens phages. 19705485742
liberation of agrobacterium tumefaciens dna from the crown gall tumor cell dna by shearing. 19705495741
[comparative study of the formation and biogenesis of beta-indoleacetic acid by agrobacterium tumefaciens and its fixed l forms]. 19705499549
comments on the results of the preceding publication of manigault concerning agrobacterium tumefaciens m39 mutant. 19705512790
some characteristic properties concerning agrobacterium tumefaciens m39 mutant. 19705519706
d-glucaric acid and galactaric acid catabolism by agrobacterium tumefaciens.cell-free extract (crude extract) of agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. these compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. the same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine ...19704314480
[3. the influence of rifampicin on tumor induction by agrobacterium tumefaciens]. 19704394213
[iv. rifampicin and a resistant clone of agrobacterium tumefaciens in tumor induction]. 19704395544
electron microscopy of phages for agrobacterium tumefaciens. 19704098556
[the chlorophyll content of tumors from pale and green leaf tissues of kalanchoe fedtschenkoi].pale and green leaves of kalanchoe fedtschenkoi var. variegata were inoculated with agrobacterium tumefaciens. six weeks later the chlorophyll contents of the leaves and their tumors were investigated. the tumor tissues possess very similar concentrations of chlorophylls and a low number of chloroplasts per cell. thereby they differ from each of the initial tissues. the qualitative composition of pigments (chlorophylls and carotenoids) of the 4 tissues is the same.197024500130
purification & properties of a soluble reduced nicotinamide adenine dinucleotide (nadh) dehydrogenase from agrobacterium tumefaciens. 19714146370
the loss of tumor-initiating ability in agrobacterium tumefaciens by incubation at high temperature. 19715544763
conditions for production of 3-ketomaltose form agrobacterium tumefaciens. 19715549698
agrobacterium tumefaciens ribonucleic acid synthesis in tomato cells and crown gall induction.purified agrobacterium tumefaciens deoxyribonucleic acid (dna) does not produce crown gall tumors in growing plants, conditioned by wounding, as the living bacteria do. purified bacterial dna migrates in the plant and replicates, but it is not transcribed in our experimental conditions. on the contrary, when dna is released naturally from bacteria into plant cells, a bacterial ribonucleic acid (rna) can be found in these cells. there seems to be a direct relation between the appearance of a. tum ...19715573736
serological studies with agrobacterium radiobacter, a.tumefaciens, and rhizobium strains. 19714999394
conditions for production of 3-ketomaltose from agrobacterium tumefaciens.up to 39% yields of 3-ketomaltose were achieved in 18 to 22 hr when agrobacterium tumefaciens nrrl b-36 was cultured at 25 to 28 c in a simple medium containing 4.0 to 8.0% maltose, 0.09% urea, 0.5% caco(3), 0.6% kh(2)po(4), and 0.025% mgso(4).7h(2)o. for maximum production of 3-ketomaltose the culture had to be maintained approximately at ph 7.0.19714925530
a selective medium for agrobacterium radiobacter biotype 2. 19714935439
[induction of tumors by the thermostable endotoxin of agrobacterium tumefaciens on datura stramonium l]. 19715129961
[structure and chemical composition of the lipopolysaccharide of agrobacterium tumefaciens]. 19715159956
tumor induction by agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue.when agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (dna) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. such association is not observed in identical experiments when a. tumefaciens cell ribonucleic acid or protein are labeled. the extent of the retention of thymidine-derived label from bacterial cells by t ...19715167810
evidence for the presence of bacteria-specific proteins in sterile crown gall tumor tissue.cross-reacting antigens were found in bacteria-free crown gall tumor tissue tested with serum prepared against agrobacterium tumefaciens (smith and towns.) conn., but no such antigens were detected in callus tissue. soluble proteins from tumor tissue, callus tissue, and the crown gall bacteria were fractionated on a deae-sephadex (a-50) column. the diethylaminoethyl-sephadex elution profile for tumor tissue showed three protein fractions that were not detected in the callus tissue. two of these ...197116657748
 two auxin oxidases with distinct antigenic specificities have been identified in extracts of plant tumor induced in datura stramonium by agrobacterium tumefaciens. auxin oxidase and peroxidase activities are demonstrated for both enzymes. the adaptation of an immunochemical method allows the quantitative evaluation of both enzymes in different plant tissue extracts. the amount of these enzymes which is low in the organ of the healthy plant increases during tumorisation, healing and in vitro cult ...197111945769
promotion of crown-gall tumor growth by lysopine, octopine, nopaline, and carnosine.the growth of crown-gall tumors on primary bean leaves (phaseolus vulgaris l. cv. "pinto") was promoted by the addition of d-lysopine, d-octopine, l-carnosine, or nopaline. assayed on tumors induced by agrobacterium tumefaciens strain b6, the relative activity was octopine = carnosine > lysopine >> nopaline; assayed on tumors induced by a. tumefaciens strain t-37, which induces tumors which form nopaline, the relative activity was nopaline = octopine = carnosine > lysopine. from one to three app ...197216657911
degradation of lysopine by an inducible membrane-bound oxidase in agrobacterium tumefaciens. 197211946839
transformation of agrobacterium tumefaciens into a non-oncogenic species by an escherichia coli rna.transforming rna excreted by showdomycin-resistant escherichia coli induces a persistent, heritable, and spectacular change in agrobacterium tumefaciens b(6), a bacterium that carries the oncogenic principle for tumor induction in plants. transformants possessing new physiological and biochemical properties have completely or partially lost the capacity for tumor induction. they synthetize new ribosomes whose components are profoundly modified. on the basis of biological and biochemical characte ...19724550504
isolation of morphological mutants of agrobacterium tumefaciens.morphological mutants were isolated from a wild strain of agrobacterium tumefaciens at a high frequency by treatment with a nitrosoguanidine. seventeen of the 20 mutants isolated were temperature-sensitive. at 27 c, the mutant cells were rod-shaped and at 37 c, spherical or branched, whereas the wild-type cells were rod-shaped at both temperatures.19724553841
transfection in agrobacterium tumefaciens.intact cells of agrobacterium tumefaciens were examined for ability to take up biologically active lr-4 phage deoxyribonucleic acid (dna) from the surrounding medium. dna incorporation as measured by subsequent plaque formation (transfection) failed to occur when the bacteria were grown in defined minimal salts media, and was restricted to a 4-hr period in the early log phase of growth in enriched media. in the latter case, maximal transfection frequencies were obtained after a 25- to 30-min inc ...19725040385
[various tarnished facts about agrobacterium tumefaciens]. 19725068500
host-bacteriophage interaction in agrobacterium tumefaciens. i. characterization of bacteriophage r4 and physiological changes in the infected host cell.infection of agrobacterium tumefaciens b6, a tumor-producing plant pathogen, by bacteriophage r4, does not immediately shut off host deoxyribonucleic acid (dna), ribonucleic acid (rna), and protein synthesis. viral dna synthesis begins soon after infection, but the host dna is not shut off until after 35 min; net rna and protein synthesis are not inhibited until 30 min after infection. the pattern of synthesis of phage particles was confirmed by electron microscopy of thin sections during the in ...19725084466
[effect of bacterial attenuation on expression of the oncogenic capacity of agrobacterium tumefaciens]. 19724628302
[relationship between l-asparaginase activity and the oncogenic power of different agrobacterium tumefaciens mutants (smith and town.) conn]. 19724630665
[loss of virulence associated with loss of an enzyme activity in agrobacterium tumefaciens]. 19724631534
ultrastructure of stable l phase of agrobacterium tumefaciens causing tumor fromation in phaseolus vulgaris l. 19724663991
[structure and chemical composition of lipopolysaccharides of l forms of agrobacterium tumefaciens]. 19724664780
electron microscopy of lipopolysaccharides from erwinia carotovora and the bacillary and l forms of agrobacterium tumefaciens. 19724673374
morphological, biological, and physical properties of agrobacterium tumefaciens bacteriophages. 19724110281
[rifampicin and the agrobacterium tumefaciens phage ps8]. 19724403419
[ -amanitin inhibits tumor induction by agrobacterium tumefaciens]. 19724405303
control of pantothenate accumulation in agrobacterium tumefaciens.two pantothenate-requiring mutants of agrobacterium tumefaciens have been isolated. one of them (strain wmp-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. extracts of cells grown on limiting aspartate were found to contain four times more (14)c-pantothenate than those grown at optimal aspartate concentrations. this difference was found in ...19734347924
transmissible resistance to penicillin g, neomycin, and chloramphenicol in rhizobium japonicum.the genetic basis for resistance to a number of antibiotics was examined in rhizobium japonicum. resistance to penicillin g, neomycin, and chloramphenicol appears to be mediated by an extrachromosomal element similar to that found in the enterobacteriaceae. resistance to these antibiotics was eliminated from cells by treatment with acridine orange, and resistance to all three antibiotics could be transferred en bloc to agrobacterium tumefaciens under conditions excluding transformation or transd ...19734491197
a soluble cytochrome c from agrobacterium tumefaciens, strain b2a. 19734128307
"genetic' transformation of bacteria by rna and loss of oncogenic power properties of agrobacterium tumefaciens. transforming rna as template for dna synthesis. 19734137363
3-ketoglucose reductase of agrobacterium tumefaciens.two kinds of 3-ketoglucose-reducing enzyme were partially purified from the sonic extract of agrobacterium tumefaciens iam 1525 grown on a sucrose-containing medium. both enzymes have a specific requirement for reduced nicotinamide adenine dinucleotide phosphate (nadph) as a hydrogen donor and catalyze the reduction of 3-ketoglucose to glucose but do not reduce 3-ketoglucosides such as 3-ketosucrose, 3-ketoglucose-1-phosphate, 3-ketotrehalose, and 3-ketocellobiose. from the requirement and subst ...19734144143
[bacterial cell wall and tumor induction by agrobacterium tumefaciens (author's transl)]. 19734271607
investigations implying the invalidity of octopine as a marker for transformation by agrobacterium tumefaciens. 19734273701
[relationship between l-asparaginase activity and the oncogenic power of different agrobacterium tumefaciens mutants (smith and town.) conn]. 19734684029
on the question of crown-gall tumor initiation by dna of bacteriophage ps8.dna of agrobacterium tumefaciens bacteriophage ps8 was isolated by using several procedures. whole phage and phage dna were tested for tumor-inducing ability on 10 species of plants with various additions to assist such activity. the reported tumorigenicity of phage ps8 dna could not be confirmed, and no evidence to implicate phage ps8 involvement in tumor initiation was obtained.19734688706
glucose-1-phosphate-negative mutant of agrobacterium tumefaciens.glucose-1-phosphate-negative mutants that are unable to grow in a synthetic medium containing glucose-1-phosphate (g-1-p) as a sole carbon source were isolated by treatment of agrobacterium tumefaciens iam 1525 with n-methyl-n'-nitro-n-nitrosoguanidine. all of the enzymes involved in g-1-p metabolism (glucoside-3-dehydrogenase, 3-ketoglucose-1-phosphate-degrading enzyme, alpha-glucosidase, and phosphatases) were detected in the sonic extract prepared from resting cells of one of the mutants, str ...19734690962
the presence of both phage ps8 and agrobacterium tumefaciens a 6 dna base sequences in a 6 -induced sterile crown-gall tissue cultured in vitro. 19734691351
[specific degradation of octopine and arginine by virulent agrobacterium tumefaciens b6]. 19734725823
carbohydrate metabolism in agrobacterium tumefaciens.the activity of pentose cycling (pc) reactions in agrobacterium tumefaciens is much greater than that normally found in bacteria, and in this regard the organism represents a unique category. equations specifically derived from radiorespirometric data for bacteria with high pc activity in the presence of an alternate pathway are presented. a. tumefaciens utilizes d-glucose by strictly aerobic mechanisms involving the entner-doudoroff (ed) and pc pathways; relative participation by the ed pathway ...19734745418
["agrobacterium tumefaciens" (smith and town) conn.: minimal inhibitory concentrations of various antibiotics and sulphonamide (author's transl)]. 19734748294
induction of crown gall by nitrosoguanidine-treated agrobacterium tumefaciens. 19734753932
purification and properties of glucose i-phosphate-binding protein in agrobacterium tumefaciens. 19734761987
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