Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| comparison of an enzyme-linked immunospecific assay (elisa) with the cold complement fixation test for the serodiagnosis of brucella ovis infection. | an enzyme-linked immunospecific assay (elisa) for the serodiagnosis of brucella ovis infection in sheep is described and compared with the cold complement fixation (cf) test. elisa was performed in microtiter plates, using horse-radish peroxidase conjugated to anti-normal sheep serum globulins, and hydrogen peroxide plus o-phenylenediamine as substrate. a heated, cell-free b. ovis extract was used as antigen in both tests. elisa was easier to perform, distinguished better between positive and ne ... | 1984 | 16031008 |
| a comparison of three serological tests for the diagnosis of b. ovis infection in rams. | the complement fixation test (cft), the enzyme labelled immunosorbent assay (elisa) and the gel diffusion precipitin test (gd) were compared, for the diagnosis of brucella ovis infection in rams. the sensitivities of the tests in 109 rams which were shedding b. ovis in their semen were: cft 96.3%; elisa 97.2%; gd 91.7%. the specificities of the tests in 141 rams from non-infected flocks were: cfi 99.3%; elisa 98.6%; gd 100%. predictive values of the three tests were measured in 285 rams from inf ... | 1984 | 16031047 |
| serology and semen culture for the diagnosis of brucella ovis infection in chronically infected rams. | the serological response to brucella ovis and the shedding of the organism in semen was followed for a period of 13-14 months in 42 naturally infected rams. most rams remained chronically infected and excreted the organism in their semen throughout the investigation. b. ovis was isolated from 87.9% of the semen samples from the infected rams. the most common sites from which b. ovis could be isolated at necropsy were the epididymides and accessory sexual glands. in one ram the organism was isola ... | 1985 | 16031169 |
| ovine brucellosis: repeated vaccination with rev 1 vaccine and the prevalence of the disease in the winburg district. | the effect of repeated vaccination of ram lambs with brucella melitensis rev 1 vaccine was studied in relation to complement fixation titres. the presence of brucella organisms in semen and changes in neutrophil densities of the semen were also studied. a survey of the incidence of ovine brucellosis in the winburg district on the basis of a clinical examination of rams and the complement fixation test is also reported on. repeated vaccination of ram lambs with rev 1 vaccine resulted in high comp ... | 1985 | 3836306 |
| evaluation of an enzyme linked-immunosorbent assay for the diagnosis of brucella ovis infection in rams. | a simple enzyme linked immunosorbent assay (elisa) was developed for the serological diagnosis of brucella ovis infections in rams. serums from brucellosis accredited-free flocks and flocks known to be infected with b. ovis were tested and the results correlated with warm complement fixation (cf) test and bacteriological examination of semen. both the elisa and the cf test detected 0.5% false positive reactions in rams from clinically negative flocks. however the elisa detected significantly mor ... | 1985 | 3893408 |
| use of phage for the identification of brucella canis and brucella ovis cultures. | the brucella phage strains r, r/o and r/c standardised at routine test dilution on their propagating strains were effective in identifying cultures of brucella ovis and b canis and in differentiating these from other non-smooth brucella isolates. | 1985 | 3975479 |
| [comparative studies of the properties of br. ovis strains 03, 303 and 100671 to those of local strains for the production of antigen]. | colorimetric analysis, biochemical methods, and the complement-fixation test were employed to study 3 australian and 5 bulgarian strains of brucella ovis. determined were some biochemical manifestations, the adaptation capacity with regard to aerobic conditions of cultivation without serum, the amount of polysaccharides contained in the strains, and the properties of the antigens obtained as checked via cft. milanov's method was employed for antigen production. three of the bulgarian strains sho ... | 1985 | 3992937 |
| prevalence and causes of ram epididymitis in utah. | palpation of the testicles and epididymides was used to determine the prevalence of ram epididymitis (re) in utah range flocks over a 5-year period. of 62 range flocks examined, 58 had rams with palpable lesions. a comparison was made of prevalence by right vs left testicle involvement, but no significant difference was found. culling all rams with palpable lesions from the flock progressively reduced the annual incidence, but did not eliminate re from any flock. semen and specimens of reproduct ... | 1985 | 3997640 |
| use of enzyme-linked immunosorbent assay for detection of antibodies to brucella ovis in sheep: field trial. | an enzyme-linked immunosorbent assay (elisa), using an autoclave-extracted soluble antigen, for the detection of serum antibodies to brucella ovis in sheep was developed. the test seemed to be both sensitive and specific, on the basis of the control groups studied. the antigen showed no deterioration in prepared plates stored at -70 c for up to a year. the elisa was used in conjunction with palpation of rams for epididymal lesions as a means to detect and control b ovis infection in a naturally ... | 1985 | 4037490 |
| susceptibility of male white-tailed deer (odocoileus virginianus) to brucella ovis infection. | infection with brucella ovis was established by conjunctival instillation in 8 male white-tailed deer (odocoileus virginianus). infection was transient in young bucks, but persisted in bucks that were mature when inoculated. the deer were euthanatized and necropsied at various intervals after inoculation. brucella ovis was recovered from a mature buck at necropsy on postinoculation day 429. four deer had gross lesions and histopathologic changes of the epididymides. a mature noninfected buck con ... | 1985 | 4037505 |
| experimental infection of goats with brucella ovis. | six goats were inoculated with brucella ovis. two goats were inoculated with infected semen by the intratesticular route and 2 each by installation of the semen on to the nasal or preputial epithelium. all goats produced antibody responses as measured by an enzyme-linked immunosorbent assay (elisa) procedure and the serums of 5 goats reacted in complement fixation tests for b. ovis. the 2 goats inoculated by the intratesticular route and one receiving b. ovis instilled intranasally subsequently ... | 1985 | 4062738 |
| restriction endonuclease analysis of brucella ovis and other brucella species. | brucella ovis dna was analysed by using 11 different restriction endonucleases. the most clearly resolved dna fragment patterns were obtained after digestion with the enzyme hind iii. when dna preparations from 35 strains of b. ovis were digested with this enzyme, the fragment patterns appeared to be identical. the patterns obtained after hind iii digestion of dna from one strain each of b. abortus, b. canis and b. melitensis were more similar to each other than to the b. ovis pattern. | 1985 | 2996213 |
| epididymitis caused by brucella ovis in a southern ontario sheep flock. | epididymitis was diagnosed in three rams in a commercial sheep flock in southern ontario. the affected rams had palpably enlarged epididymides and two rams had semen which contained inflammatory cells and was of poor quality. serum compliment fixation titers for brucella ovis were 1:20, 1:80 and 1:90. five other rams in the flock were clinically normal and without titers. two of the affected rams had lesions similar to those produced by experimental infection with b. ovis. the infection in the r ... | 1985 | 17422577 |
| ovine brucellosis in alberta. | two parallel surveys of rams from alberta sheep flocks were conducted to determine the presence of infection with brucella ovis. in a retrospective study over a period of 24 months, using complement fixation test, 12 flocks out of 142 tested were considered infected. in another 17-month survey of slaughter rams by serology and culture methods 11 flocks out of 124 were found to be infected. the overall prevalence of ovine brucellosis was 8.6% of the flocks tested which represented 12.5% of the es ... | 1986 | 17422669 |
| etiologic significance of bacterial isolates from rams with palpable epididymitis. | identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ... | 1986 | 3521407 |
| comparison of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection. | brucella ovis hot saline extracts and petroleum ether-chloroform-phenol lipopolysaccharide were compared in an enzyme-linked immunosorbent assay for the diagnosis of b. ovis ram epididymitis. hot saline extracts detected greater numbers of infected rams. chemical characterization of the antigens showed that, although both contained lipopolysaccharide, hot saline extracts also contained outer membrane proteins. these proteins were active as antigens in western blot tests with sera of infected ram ... | 1986 | 3711279 |
| the influence of supplemental vitamins a and e on ovine humoral immune response. | these experiments determined if supplemental vitamins a and/or e would enhance ovine antibody responses. all-rac-alpha-tocopheryl acetate was fed to lambs approximately 6 months old (30 to 40 kg) at levels of 33 (controls), 121, 276, 396, and 476 mg/kg of feed (which are total vitamin e levels). primary and secondary immunizations with 10 mg keyhole limpet hemocyanin (klh) were given. a nonlinear dose response of serum antibody titers was observed and the 476 mg vitamin e/kg treatment significan ... | 1986 | 3714722 |
| correlation of the presence of seminal white blood cells and the prevalence of separated spermatozoal heads with subclinical brucella ovis infection in rams. | a breeding soundness evaluation was conducted on 824 colorado range rams. these rams were determined to be free from epididymitis via testicular palpation. semen evaluation included microscopic observation for the presence of wbc. of the 824 rams, 15.5% failed the breeding soundness evaluation on the basis of the semen evaluation: 10.6% had wbc in the semen and 4.9% had poor sperm morphology. the prevalence of brucella ovis isolation varied from 0% to 16.2% within flocks. the prevalence of subcl ... | 1986 | 3733506 |
| demonstration of a peptidoglycan-linked lipoprotein and characterization of its trypsin fragment in the outer membrane of brucella spp. | the sodium dodecyl sulfate (sds) extraction-trypsin digestion protocol used by braun and sieglin (v. braun and u. sieglin, eur. j. biochem. 13:336-346, 1970) to show the peptidoglycan-linked lipoprotein of escherichia coli was applied to both brucella abortus and e. coli. whereas a single polypeptide of 8,000 molecular weight was obtained from e. coli, several proteins of apparent molecular weight lower than 35,000 were demonstrated by sds-polyacrylamide gel electrophoresis in b. abortus. these ... | 1986 | 3744559 |
| identification of immunoreactive membrane antigens of brucella ovis by elisa profiling. | enzyme-linked immunosorbent assay (elisa) profiling was used to identify the immunoreactive membrane antigens of brucella ovis. immunoreactive membrane antigens obtained after detergent extraction of the bacterial membrane complex (inner and outer membranes) were resolved into five peaks (a, b, b1, c and d) by gel permeation chromatography. aliquots from each of the chromatographed fractions were coupled to 96-well microtitre plates and immunoreactive fractions identified with sera from two rams ... | 1986 | 3764093 |
| immune response in rams experimentally infected with brucella ovis. | cellular as well as humoral immune responses were detected in six rams experimentally infected with brucella ovis. specific antibodies were detectable by enzyme-linked immunosorbent assay by day 11 after infection in all the rams. the levels of igm antibodies and total antibodies in the serum rose until 33 and 41 days after infection respectively, then levelled off. antigen-induced blastogenic responses by lymphocytes developed as early as five days after infection in all rams but had decreased ... | 1986 | 3764107 |
| examination of rams culled during an ovine brucellosis accredited free flock scheme. | during the early stages of an ovine brucellosis accredited free flock scheme in new south wales 62 rams were examined to determine the status of their flocks of origin with regard to infection with brucella ovis. forty rams were selected because they were either single reactors to low titre or one of a small number of reactors in a b. ovis complement fixation test of the whole ram flock. twenty two rams were selected because they had palpable abnormalities within the scrotum but were negative se ... | 1986 | 3778368 |
| serological, bacteriological and pathological changes in rams following different routes of exposure to brucella ovis. | mature merino rams were exposed to brucella ovis by contact with infected semen, using either ewe transmission, intrapreputial, intranasal or intrarectal inoculation of infected semen or intrapreputial inoculation of b. ovis culture. thirty-six of the 41 rams developed significant complement fixation (cf) test titres, but only 9 of these reactors showed clinical, bacteriological or pathological evidence of infection. infection occurred in some of the rams from all groups. the results are discuss ... | 1986 | 3800795 |
| association of age of ram with distribution of epididymal lesions and etiologic agent. | a 2-year study of the frequency of isolation of various organisms from mature and yearling rams with epididymitis was conducted at the us sheep experiment station at dubois, idaho. investigation into the distribution of lesions in the epididymis in relation to age of the ram also was studied. serologic or bacteriologic evidence of brucella ovis infection was demonstrated in 79.5% of the mature rams with epididymal lesions. actinobacillus seminis and histophilus ovis were the 2 most frequently is ... | 1986 | 3949615 |
| distribution of brucella ovis in the tissues of rams reacting in a complement fixation test for ovine brucellosis. | 1986 | 3954693 | |
| detection of antibody in rams with contagious epididymitis, using the enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay, using whole-cell and sonicated antigens prepared from brucella ovis, actinobacillus seminis, and actinobacillus seminis-like cultures isolated from rams in wyoming, was able to detect antibody to these antigens in rams with epididymitis. the whole-cell antigens used in this procedure gave lower background values, compared with those of the sonicated antigens. the procedure was able to detect antibody in rams before clinical signs of epididymitis became appar ... | 1986 | 3963577 |
| [discovery of rams shedding brucella ovis by immunochemical study of the seminal plasma]. | clinical, bacteriological, serum and immunochemical experiments were carried out on rams, infected with br. ovis. the most advisable for the discovery of infectious rams proved to be the immunochemical research on the seminal plasma. the demonstration of immunoglobulin fractions in the seminal plasma of rams of the infected group permits to find out the rams, detaching br. ovis, without treating the animals with the abortive form of the infection. | 1987 | 3450075 |
| a rapid slide agglutination test for the serodiagnosis of brucella canis infection that employs a variant (m-) organism as antigen. | an improved antigen is described for use in the serodiagnosis of canine brucellosis. the novel antigen employs a less mucoid (m-) variant of b. canis. the replacement of brucella ovis with b. canis (m-) cells as antigen in slide agglutination tests would increase accuracy by reducing the rate of false positive results from ca. 50% to ca. 10%. rose bengal stained b. canis (m-) cells are slightly more sensitive than the commercially available brucella ovis as slide test antigens. both test procedu ... | 1987 | 3802828 |
| diagnostic sensitivity and specificity of an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection in rams. | an enzyme-linked immunosorbent assay (elisa) for antibody to brucella ovis was compared with a standard complement fixation test. sera of 176 rams from uninfected flocks gave 175 negative and one suspect elisa reaction (diagnostic specificity 99.4%) whereas the complement fixation test yielded 167 negative, seven suspect and two anticomplementary reactions (diagnostic specificity of 96.0%). diagnostic sensitivity was evaluated on sera of 79 rams from which b. ovis had been isolated. the elisa sh ... | 1987 | 3567755 |
| economics of brucella ovis control in sheep: epidemiologic simulation model. | the epidemiology and economics of brucella ovis control in a hypothetical, commercial sheep flock (100 rams and 2,500 ewes) were investigated. the investigation consisted of an epidemiologic simulation model, reported here, and a decision-tree analysis, reported in a companion paper. the epidemiologic model was designed to simulate the transmission and persistence of b ovis in a ram flock during the mating season as well as the nonmating (isolation) season. a constant contact rate was selected f ... | 1987 | 3570957 |
| economics of brucella ovis control in sheep: computerized decision-tree analysis. | the epidemiology and economics of brucella ovis control in a hypothetical, commercial sheep flock (100 rams and 2,500 ewes) were investigated. the investigation consisted of an epidemiologic simulation model, reported in a companion paper, and a decision-tree analysis, reported here. it was predicted from the simulation model that b ovis could be eradicated successfully in 2 test periods (less than 1 year) from a flock by using intensive screening and culling. a computerized decision-tree progra ... | 1987 | 3570958 |
| the distribution of histopathological lesions in rams reacting in a complement fixation test for brucella ovis. | 1987 | 3619789 | |
| immunization with brucella melitensis rev 1 against brucella ovis infection of rams. | the efficacy of brucella melitensis rev 1 vaccine (rev 1) for the prophylaxis of brucella ovis ram epididymitis was evaluated. twenty-nine 3-month-old rams were vaccinated with 2 x 10(9) rev 1 and 14 were revaccinated with 5 x 10(8) at 14 months of age. six rams remained unvaccinated as a control group. all rams were challenged with 5 x 10(8) b. ovis at 21 months of age. before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams deve ... | 1987 | 3686824 |
| pathology of the accessory sex glands of rams infected with brucella ovis. | 1987 | 3689264 | |
| isolation and antigenic reactivity of brucella ovis outer membrane proteins. | brucella ovis cell membranes were isolated from fractured and lysozyme-treated cells by ultracentrifugation. these preparations appeared to consist largely of outer membranes, as judged from the results of ultracentrifugation experiments in sucrose density gradients under conditions that are widely used to separate inner and outer membranes of gram-negative bacteria. the sequential detergent extraction of cell membranes yielded mainly lipopolysaccharide and three groups of outer membrane protein ... | 1987 | 3693544 |
| restriction endonuclease (ecor1) analysis of brucella ovis dna. | seven brucella ovis isolates from diverse sources (including two used in the manufacture of a br. ovis vaccine, four field strains and a reference strain from the national culture type collection in london) were subjected to bacterial restriction endonuclease dna analysis using the endonuclease ecor1. no genetic differences were detected. it was concluded that strain variation is unlikely to be a problem in br. ovis control schemes, whether these are based on vaccination or on the eradication of ... | 1987 | 16031329 |
| the serological response of rams to four methods of vaccination against brucella ovis infection. | a comparative study was made of the serological response of rams to an inactivated brucella ovis saline in-oil vaccine administered either once or twice by either the subcutaneous or the intraperitoneal route. the serological response of rams to two spaced doses of vaccine was more consistent and more persistent than when a single dose of vaccine was used. the rise in titre was more rapid and the final titre of greater magnitude when the subcutaneous route of administration was used in compariso ... | 1987 | 16031388 |
| semen characteristics, scrotal circumference and bacterial isolates of fine wool range rams. | during 1983, 887 fine wool rams were subjected to breeding soundness evaluation to determine effects of age and scrotal circumference on semen characteristics. of rams evaluated, 94.4, 84.8, and 81.9% of young (</=1.5 yr), mature (2 to 5 yr), and old (>6 yr), respectively, were rated as satis-factory. old rams had fewer (p < 0.05) motile cells and more (p < 0.05) abnormal cells than young rams. among older rams there was a higher percentage (p < 0.05) with testicular lesions than among young or ... | 1987 | 16726345 |
| relationships between brucella ovis semen culture and various semen and serology parameters. | semen and blood samples from 154 rams from two montana range flocks (flock a, vaccinated for brucella ovis ; flock b, nonvaccinated) were evaluated to determine the relationship between brucella ovis (b. ovis ) semen culture results and various semen and blood parameters. all rams utilized in this study exhibited no palpable ram epididymitis lesions. thirteen and 25.6% of the rams tested in flocks a and b, respectively, had positive b. ovis semen cultures. only age of ram and ram condition score ... | 1988 | 16726417 |
| adherence of brucella ovis to preimplantation ovine ova. | seventy-three zona pellucida-intact ova were collected surgically from 15 superovulated, brucella -free mixed-breed ewes. twenty-one groups containing one to five ova were incubated in medium containing brucella ovis . subsequently, seven and five groups were incubated for 24 and 4 h, respectively, at 37 degrees c in medium containing penicillin and streptomycin, while nine groups were not treated with antibiotics. all groups of ova were washed 10 times, and ova and sequential washes were cultur ... | 1988 | 16726479 |
| local tissue reaction to the administration of an inactivated brucella ovis saline-in-oil vaccine by the intraperitoneal route. | an inactivated brucella ovis saline-in-oil vaccine was administered to 14 adult ewes using both the intraperitoneal route and the subcutaneous route. pairs of animals were necropsied at intervals between 24 hours and ten weeks after injection. the nature of the local inflammatory reaction to the administration of the vaccine was similar at all sites. the lesion consisted of granulomatous inflammation arranged around droplets of oily vaccine. diffuse peritonitis was seen at necropsy in 12 of the ... | 1988 | 16031427 |
| the response of vaccinated rams to experimental challenge using brucella ovis. | one hundred and thirty eight rams were allocated to four experimental groups. an inactivated br. ovis vaccine was administered either once by the intraperitoneal route (1 i/p), twice by the intraperitoneal route (2 i/p), or twice by the subcutaneous route (2 s/c), and the last group was left unvaccinated. they were then challenged by the intravenous inoculation of between 123 and 1.23 x 108 live br. ovis bacteria. the number of rams that succumbed to infection within the four groups was 4135 (11 ... | 1988 | 16031445 |
| characterization of brucella ovis surface antigens. | a rough antigen (sra) extracted from brucella ovis in hot saline by myers procedure, showed three precipitation lines when tested in immunodiffusion against sera from experimentally infected rams. the components responsible for the lines could be isolated by ultracentrifugation or gel filtration which gave 3 fractions, named pi, pii and piii. the lipopolysaccharide (lps) appeared in the pellet (sra-pp) after ultracentrifugation as judged by the presence of lipids, sugar composition, 2 keto-2deox ... | 1988 | 3148241 |
| [brucella ovis as a possible cause of epididymitis in angora goat rams]. | 1988 | 3210211 | |
| serodiagnosis of histophilus ovis-associated epididymitis in rams. | an elisa for the detection of antibodies to histophilus ovis was used to evaluate the association of epididymal lesions in rams with serologic response to his ovis. comparison of elisa results for his ovis in groups of rams with epididymal lesions with elisa results of clinically normal rams (control group) revealed a significant difference (p less than 0.01) between the control group and those rams from which his ovis was isolated. a significant difference (p less than 0.01) was noticed between ... | 1988 | 3348531 |
| immunoglobulins and immunoglobulin-containing cells in the reproductive tracts of rams naturally infected with brucella ovis. | thirteen rams with serological evidence of brucella ovis exposure (cft of 1:8 or greater), but with no or only mild epididymitis, were selected from a ram flock. serum, semen, preputial washings and fluids from the accessory sex glands (asgf) and testis and epididymis (tef) were examined and immunoglobulin (ig) concentrations estimated. genital tissues were examined histologically and the percentages of class specific immunoglobulin containing cells (icc) determined. eleven of these rams had his ... | 1988 | 3355449 |
| identification of brucella ovis in formalin-fixed paraffin-embedded genital tissues of naturally infected rams by the indirect peroxidase-antiperoxidase technique. | 1988 | 2461696 | |
| comparison of protein components of different species and strains of brucella. | the proteins constituents of brucella abortus, brucella melitensis and brucella ovis were analyzed sds-page. from the comparison appears that the three species of brucella studied shows a different electrophoretic pattern specially at the level of small peptides. on the contrary when two strains of b. abortus are analyzed no differences can be noticed. | 1989 | 2506718 |
| comparison of three serological tests for brucella ovis infection of rams using different antigenic extracts. | the sensitivity and specificity of the complement fixation, gel diffusion and elisa tests for the diagnosis of brucella ovis infection of rams have been compared using three different antigenic preparations. the antigens obtained by petroleum ether - chloroform - phenol, or cold saline extractions gave poorer diagnostic results than those obtained by hot saline extraction in all the tests. the best sensitivity was obtained with the elisa (97.6 per cent) followed by the gel diffusion (96.4 per ce ... | 1989 | 2512715 |
| comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of brucella ovis and brucella melitensis. | sheep infected with brucella ovis produce antibody responses to the rough lipopolysaccharide and to proteins present in hot saline (hs) extracts of b. ovis (j. i. riezu-boj, i. moriyón, j. m. blasco, c. m. marín, and r. díaz, j. clin. microbiol. 23:938-942, 1986). the distribution and antigenic relatedness of proteins in hs extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting for 41 strains of b. ovis and 26 strai ... | 1989 | 2651307 |
| efficacy of long-acting oxytetracycline alone or in combination with streptomycin for treatment of brucella ovis infection of rams. | twenty-four rams inoculated with brucella ovis by conjunctival and preputial routes were treated with a long-acting oxytetracycline alone or in combination with dihydrostreptomycin sulfate. the combined treatment eliminated brucella ovis from 11 of 12 (91.6%) treated rams. only 4 of 12 (33.3%) rams treated with oxytetracycline alone were bacteriologically negative. neither treatment resolved clinical epididymitis in 2 rams affected before treatment. many rams had pathologic lesions in the epidid ... | 1989 | 2712423 |
| discrimination between sheep antibodies to brucella melitensis and to brucella ovis. | when preparations containing smooth brucella abortus lipopolysaccharide (lps) were used as antigen in an elisa, strong positive reactions were obtained with sera from sheep infected with brucella melitensis or with brucella ovis. oxidation of the lps with sodium metaperiodate greatly reduced the extent of the cross-reactions with antisera to b. ovis, with little effect on the reactions with antisera to smooth b. melitensis. periodate oxidation of hot saline extract (hsx) antigen of b. ovis marke ... | 1989 | 2800307 |
| temporal elisa response of rams to brucella ovis following experimental infection or vaccination. | sera from rams vaccinated with antigens extracted chaotropically from brucella ovis by potassium thiocyanate treatment were used to optimise a whole-cell, enzyme-linked immunosorbent assay (celisa) and to monitor the temporal serological response of rams which had been challenged with infected semen by the intranasal or intrapreputial route. three patterns of celisa response were detected. thirteen of 15 rams intranasally challenged did not respond serologically (pattern 1 or nil response). only ... | 1989 | 2922509 |
| comparison of subcutaneous and conjunctival routes of rev 1 vaccination for the prophylaxis of brucella ovis infection in rams. | the serological response and protection conferred against brucella ovis by the rev 1 vaccine was evaluated in both adult (experiment 1) and young rams (experiment 2) vaccinated either subcutaneously or conjunctivally. in experiment 1 the rev 1 vaccine protected 55.5 per cent and 100 per cent, respectively, of subcutaneously and conjunctivally vaccinated rams against three consecutive challenges that infected 100 per cent of unvaccinated controls. in experiment 2, rev 1 protected 100 per cent of ... | 1990 | 2110377 |
| trials with an enzyme-linked immunosorbent assay (elisa) for the diagnosis of subclinical genital infections in rams caused by histophilus ovis and actinobacillus seminis. | the performance of an enzyme-linked immunosorbent assay (elisa) was evaluated in the serological diagnosis of subclinical genital infection in 6 naturally infected ram flocks and 2 experimentally infected ram hoggets. the test employs lipopolysaccharide (lps) antigen prepared by autoclaving from actinobacillus seminis and histophilus ovis. a total of 193 sheep (118 unmated virgin rams and 75 mature breeding rams) were examined clinically, serologically (by elisa) and bacteriologically (semen bac ... | 1990 | 2220189 |
| epididymitis in rams and lambs. | this article reviews lamb epididymitis and epididymitis caused by brucella ovis. pathogenesis, occurrence, signs and lesions, diagnosis, prevention, and treatment are discussed. | 1990 | 2245369 |
| polymorphism in brucella spp. due to highly repeated dna. | the species of brucella are very closely related, but brucella ovis does not express detectable amounts of a protein, designated bcsp31, that is common to the other species. we studied the lack of expression of bcsp31 by southern analysis. dnas from the b. ovis culture collection strains and field isolates were probed with a 1.3-kb hindiii fragment encoding bcsp31 of brucella abortus. the probe hybridized to a 1.6-kb hindiii fragment of all b. ovis strains tested, showing that the gene is presen ... | 1990 | 2254243 |
| extraction of membrane antigens from brucella ovis and an assessment of their serological activity by immunoblotting. | the efficacy and selectivity of chaotropic and phase-partitioning procedures for the extraction of membrane proteins from brucella ovis were compared with a standard sarkosyl method. major group 1, 2 and 3 outer-membrane proteins (omps) of b. ovis stained by coomassie blue in sds-page gels had, respectively, apparent molecular masses of 81/82 kda, 39-41 kda and 30-32 kda. the presence of these bands in the sarkosyl extract of total membrane vesicles (tmvs) indicate that the procedure failed to s ... | 1990 | 2262793 |
| profiles of serological reactivity against cytosoluble antigens of brucella ovis in experimentally infected rams. | sera from rams infected with and excreting brucella ovis in the semen (shedders), as well as from animals which had recovered from previous experimental challenge with b. ovis, were analyzed for their serological reactivities against cytosolic antigens of the bacterium. membrane vesicles, including outer and inner membrane components, were precluded from the analyses by subjecting french-pressed bacteria to ultracentrifugation. the resulting cytosolic supernatant was fractionated into four major ... | 1990 | 2279996 |
| antibody response to brucella ovis outer membrane proteins in ovine brucellosis. | hot saline extracts of brucella ovis were composed of vesicles with outer membrane proteins (omps), lipopolysaccharide, and phospholipid as constituents. extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 omps (28,500 apparent molecular weight [28.5k], 27.0k, and 25.5k) represented 81% of the total. group 1 omps and 67.0k, 22.5k to 21.5k, and 19.5k to 18.0k proteins were also detected. adsorption of immune sera wit ... | 1990 | 2298488 |
| brucella ovis excretion in semen of seronegative, clinically normal breeding rams. | a commercial flock of suffolk and suffolk-cross breeding rams was monitored for 5 years in an effort to control epididymitis caused by brucella ovis. scrotal palpation, semen evaluations, and vaccination against b ovis were used the first 3 years. serologic evaluation (complement fixation and elisa) was added the fourth year, and bacteriologic culturing was added to the program the fifth year. semen culturing in the fifth year revealed 9 (37.5%) of 24 rams were actively excreting b ovis; 6 of th ... | 1990 | 2298657 |
| brucella ovis epizootic in virgin ram lambs. | 1990 | 2329082 | |
| immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of brucella ovis. | brucella ovis rough lipopolysaccharide (r-lps) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. r-lps was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. gel-filtration of the upper phase in a column of bio-gel p-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharide units, 2-keto-deoxy-octulosonic acid (kdo), and monosaccharides. strong acid hydrolysis followed by pap ... | 1990 | 2363245 |
| antimicrobial therapy for rams with brucella ovis infection of the urogenital tract. | rams shedding brucella ovis in semen but without palpable abnormalities of the epididymides were treated with long-acting oxytetracycline for 15 days and dihydrostreptomycin for 7 days (n = 9) or conventional oxytetracycline and dihydrostreptomycin (n = 9) for 7 days. nine rams were not treated. more treated rams were considered to have satisfactory breeding soundness examination results at posttreatment weeks 3, 7, 12, and 19. nontreated rams continued to shed b ovis in semen. after treatment, ... | 1990 | 1689288 |
| genetic variation at the omp2 porin locus of the brucellae: species-specific markers. | the omp2 locus of brucella abortus is composed of two closely related genes (omp2a and omp2b) that encode, and potentially both express, homologous porin proteins. genetic variation at this locus is revealed in the form of restriction-fragment-length polymorphisms which can be used to distinguish the type strains of all six brucella species. five of the six species contain single copies of omp2a and omp2b, whereas brucella ovis appears to have two copies of the omp2a gene. the implications of th ... | 1990 | 1978222 |
| seroconversion of recipient ewes after transfer of ova exposed to brucella ovis in vitro. | zona pellucida-intact ova collected from ewes seronegative to brucella ovis were exposed in vitro to b ovis and washed 10 times in medium that contained no antibiotics. after exposure and washing, nontransferable ova were cultured for isolation of brucella , and the viable ova were transferred into seven b ovis seronegative ewes. no pregnancies resulted, thus recipient ewes were bred during the next breeding season, and blood samples were collected for bacteriological and serological examination ... | 1990 | 16726896 |
| ontario. brucella ovis, une cause d'avortement chez le mouton. | 1991 | 17423724 | |
| comparison of seroreactivity of rams with brucellosis in a complement fixation test, whole cell elisa and by immunoblotting. | in rams with ovine brucellosis, a high degree of serological correlation exists between the complement fixation (cf) test which utilises antigen extracted from bacteria with hot saline, and the elisa reactivity using methanol-fixed brucella ovis as the assay reagent. since the whole cell elisa (celisa) detects mainly antibodies against surface antigens of b. ovis, it was concluded that the similar findings of the two serological tests is due in part to the presence of membrane antigens in the cf ... | 1991 | 2024447 |
| serological responses of rams to a brucella ovis-vitamin e adjuvant vaccine. | rams which were vaccinated at 6-8 months of age with a water-in-oil brucella ovis-vitamin e adjuvant vaccine had significantly higher serum antibody levels than rams vaccinated with a commercial b. ovis bacterin or b. melitensis rev 1. the adjuvant vaccine did not cause abscesses at the site of injection as some water-in-oil emulsions do. two years after vaccination, the vitamin e adjuvant-vaccinated rams had higher antibody level than the other groups. this was most likely due to a secondary re ... | 1991 | 2058270 |
| immunochemical studies on a brucella ovis specific protein antigen. | a brucella ovis surface protein antigen (p-ii), obtained by gel filtration with sepharose 4b of a hot saline extract was characterized. the analysis of p-ii over gradient sodium dodecylsulfate electrophoresis yielded an 18.5 and a 20 kda band. in a radioimmunoprecipitation assay using p-ii labeled with 125i, the antigen reacted specifically only with sera from rams experimentally infected with a naturally occurring rough strain of b. ovis and did not react with sera from rams experimentally infe ... | 1991 | 2063544 |
| myofibroblasts and myoepithelial cells in the chicken harderian gland. | an electron microscopic study of the myoepithelial cells in the chicken harderian gland provides evidence that these cells can be transformed into myofibroblasts. after the application of a brucella ovis suspension in sterile saline onto the eyeball, every 5 minutes for half an hour, myoepithelial cells gradually develop over a 90-minute period the characteristic features of myofibroblasts: bundles of intracytoplasmic microfilament; abundant rough endoplasmic reticulum; prominent golgi complex; ... | 1991 | 1810530 |
| [comparative study of culture media for isolating brucella ovis]. | for the isolation of brucella ovis, modified thayer-martin medium with the addition of 0.01 micrograms/ml of trimethoprim and 100 iu of nystatin (tmm) was compared with skirrow agar (sk). using viable counting technique, 11 strains were evaluated and the results were compared with those using columbia base agar with bovine blood 7% (cba). ninety-four semen samples of 33 rams from a flock with infection antecedents were cultured on the same media. growth of brucella ovis strains was similar in al ... | 1991 | 1815277 |
| observations on the eradication of brucella ovis infection from a ram flock. | the measures taken to eradicate brucella ovis infection from a naturally infected flock of 64 rams are described. lesions of epididymitis were detected in 18 rams, all of which gave either positive or suspicious reactions in the complement fixation test. a further 20 rams gave serological reactions in the complement fixation test. subsequently, semen was collected from 14 of these 20 rams and b. ovis was cultured from the semen of all 14 rams. serum samples from two rams failed to react in the c ... | 1991 | 16031612 |
| cloning and characterization of the brucella ovis heat shock protein dnak functionally expressed in escherichia coli. | the brucella ovis dnak gene, homolog to the eukaryotic hsp70 genes, was cloned by using a drosophila melanogaster probe. comparison of b. ovis and escherichia coli sequences revealed a similar organization for the dnak and dnaj genes and putative regulatory signals. in e. coli transfected with the cloned fragment, b. ovis hsp70 was expressed at 30 and 50 degrees c apparently under the control of its own promoter. the recombinant protein and a b. ovis native protein displaying the same molecular ... | 1992 | 1459952 |
| serological and necropsy findings for rams infected with brucella ovis which were not identified by the complement fixation test. | the eradication of brucella ovis from a commercial flock of 36 romney rams was complicated by four infected rams remaining undetected despite four successive flock examinations using the complement fixation test. these four rams were subsequently tested using an enzyme-linked immunosorbent assay and a gel diffusion test and shown to be infected by semen culture. all four rams could have been identified as infected at the initial test if the enzyme-linked immunosorbent assay had been used in addi ... | 1993 | 16031700 |
| use of the double immuno gel diffusion test and the enzyme-linked immunosorbent assay to distinguish false from true reactors in the complement fixation test for brucella ovis. | a gel diffusion test with sonicated brucella ovis antigen and an enzyme-linked immunosorbent assay based on heat-extracted antigen were used to distinguish false from true reactions in a complement fixation test based on heat-extracted antigen. of 142 complement fixing reactors (occurring in supposedly brucella ovis-free, accredited flocks), the gel diffusion test correctly identified the status of 139 animals as compared to 128 with the enzyme-linked immunosorbent assay. a combination of the tw ... | 1993 | 16031707 |
| identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies. | an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ... | 1993 | 8126444 |
| the dangers of disease transmission by artificial insemination and embryo transfer. | this review summarizes the major infectious diseases of the three major agricultural species (cattle, sheep and pigs) and horses, and presents the evidence for and against the possibility of infectious agents being transmitted between animals via the venereal route or by the use of semen or early embryos in commercial artificial insemination (ai) or embryo transfer (et). cattle feature most prominently in the widespread distribution of frozen semen, and national and international organizations h ... | 1993 | 8221041 |
| sequence and characterization of an insertion sequence, is711, from brucella ovis. | the nucleotide (nt) sequence of a previously discovered insertion in brucella ovis was determined and found to have the hallmarks of an insertion sequence (is). the element, designated is711, of 842 bp, is similar in g + c content to that of the brucella genome and is bounded by 20-bp imperfect inverted repeats (ir). the element appears to duplicate the nt ta of a consensus target site, ytar (r, purines; y, pyrimidines). when the complete nt sequence of four elements and 300 bp of the 3' ends of ... | 1993 | 8224885 |
| evaluation of whole cell and subcellular vaccines against brucella ovis in rams. | five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ... | 1993 | 8236802 |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| avridine and lps from brucella ovis: effect on the memory induced by foot-and-mouth disease virus vaccination in mice. | foot-and-mouth disease is one of the more economically important diseases among meat-producing biungulate species. in contrast to natural infection, current foot-and-mouth disease virus (fmdv) vaccines, prepared with inactivated virus and adjuvants, elicit short-lived protection. the immunomodulating effect on fmdv vaccines of avridine and lipopolysaccharide of brucella ovis (lps) was tested in a murine model. the duration of immunity, protection, stimulation of immunocompetent cells producing a ... | 1993 | 8296482 |
| characterization of an 18-kilodalton brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis. | some anticytoplasmic protein monoclonal antibodies (mabs) from mice immunized by infection with brucella ovis cells have been obtained. one of these mabs, bi24, was used to purify by immunoaffinity a protein with a pi of 5.6 and a molecular mass of 18 kda. this protein was present in all of the rough and smooth brucella species studied, but it could not be detected in yersinia enterocolitica 09. three internal peptides of this protein were partially sequenced; no homology with other bacterial pr ... | 1993 | 8370742 |
| evaluation of vaccines and of antigen therapy in a mouse model for brucella ovis. | a mouse model was developed to study brucella ovis infection. the evolution of the number of b. ovis per spleen of mice inoculated intravenously, intraperitoneally or subcutaneously was found to be independent of the sex of the mice. the number of b. ovis increased in the spleen when increasing the challenge dose up to 1.7 x 10(7). at higher doses of challenge, the response remained constant. in this model it was observed that the inoculation of brucella melitensis rev 1 vaccine or subcellular b ... | 1993 | 8427038 |
| [brucella ovis infection in rams of the "white alp" breed. case report]. | the clinical findings of seven "white alp" rams are discussed. six of the animals contracted brucella ovis and exhibited signs of illness. the seventh animal indicated no brucella ovis contact. the ejaculates of all rams showed significant changes. a spermatocele, typical for brucella ovis, could be proven, pathologically and anatomically, in only one case. brucella ovis was isolated from two samples. a brief discussion on the treatment of herds infected with brucella ovis is included. | 1993 | 8456270 |
| protective immunity to brucella ovis in balb/c mice following recovery from primary infection or immunization with subcellular vaccines. | experiments were performed with balb/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (hs) of b. ovis. mice convalescing from a primary infection with b. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks ... | 1994 | 8300219 |
| brucella ovis infection in two flocks of sheep. | brucella ovis infection could not be eradicated from two flocks of sheep despite serologically testing and culling the infected rams for four years. the hypothesis that the ewes played a major role in the maintenance of the infection in both flocks was investigated by using serological, bacteriological and pathological criteria. specific antibodies against b ovis were demonstrated in 71 ewes in the two flocks. forty-four of the seropositive ewes were slaughtered for bacteriological and pathologi ... | 1994 | 7810048 |
| bacteriologically confirmed cases of ovine epididymo-orchitis caused by brucella ovis in sub-carpathia. | during the eradication of brucella ovis infection from five large breeding ram flocks of sub-carpathia (the ukraine), the genital organs of 55 rams culled because of seropositivity in the agar-gel precipitation (agp) test and elisa were subjected to gross pathological, histopathological and bacteriological examination. the results of these examinations, as well as the properties of b. ovis strains isolated for the first time in the region are reported. thirty-three out of the 55 pairs of epididy ... | 1994 | 7810398 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| transmission of brucella ovis by ewes. | 1994 | 7886906 | |
| vaccination with brucella abortus rough mutant rb51 protects balb/c mice against virulent strains of brucella abortus, brucella melitensis, and brucella ovis. | vaccination of balb/c mice with live brucella abortus rb51, a stable rough mutant, produced protection against challenge with virulent strains of brucella abortus, brucella melitensis, and brucella ovis. passive-transfer experiments indicated that vaccinated mice were protected against b. abortus 2308 through cell-mediated immunity, against b. ovis pa through humoral immunity, and against b. melitensis 16m through both forms of immunity. live bacteria were required for the induction of protectiv ... | 1994 | 7927779 |
| a sensitive immunoblotting technique for the serodiagnosis of brucella ovis infections. | a simplified electrophoretic immunoblotting technique based on antigen extracted from brucella ovis cells with sodium dodecyl sulfate/mercaptoethanol was compared with the complement fixation test (cft), the enzyme-linked immunosorbent assay, and the gel diffusion test. sera from 89 chronically infected, semen culture-positive rams, 378 sera from b. ovis-infected flocks, 300 sera from accredited disease-free flocks, and 29 sera from specific-pathogen-free sheep were used. the immunoblotting tech ... | 1994 | 8068750 |
| complementation of a dnak-deficient escherichia coli strain with the dnak/dnaj operon of brucella ovis reduces the rate of initial intracellular killing within the monocytic cell line u937. | facultatively intracellular bacteria express heat shock proteins after phagocytosis by macrophages. using non-pathogenic escherichia coli strains and the human monocytic cell line u937, we showed that deletion of the dnak gene significantly increased the rate of initial intracellular killing of bacteria. trans-complementation of the deletion mutant with the dnak/dnaj operon of brucella ovis restored the pattern of intracellular elimination of the control strain expressing dnak. these differences ... | 1994 | 8076809 |
| eradication of brucella ovis from the falkland islands 1977-1993. | a campaign to eradicate brucella ovis from sheep in the falkland islands, using a combination of serological tests and culling, was initiated in 1977 and continued until 1993 when, after a total of 65,266 tests had been carried out, the organism had been eradicated from the national flock. the paper discusses the relative values of the serological tests and suggests guidelines for maintaining the flock free of the infection. | 1994 | 8085323 |
| seroprevalence of brucella ovis infection in commercial ram flocks in the tamworth area. | commercial ram flocks in the tamworth area of northern new south wales were surveyed to estimate the proportion of flocks, and rams, infected with brucella ovis. the flock prevalence (percentage of flocks containing seropositive rams) of 9.1% for merino flocks was significantly lower than that for british-breed flocks (43.8%, p=0.006) and mixed-breed flocks (46.7%, p=0.017). the mean flock prevalence over all flock types was 32.9%. these estimates were supported by data obtained from diagnostic ... | 1994 | 16031755 |
| diagnosis of brucella ovis infection of rams with an elisa using protein g as conjugate. | the complement fixation and gel diffusion tests for brucella ovis ram epididymitis were compared with an indirect elisa using antigens from b ovis extracted in hot saline, rough b ovis lipopolysaccharide or a cytosolic fraction of rough b melitensis 115, and commercial anti-igg (heavy and light chain specificity) or protein g conjugates. none of the antigens and conjugates used in the elisa gave better results in terms of sensitivity and specificity than the complement fixation or the gel diffus ... | 1995 | 8540208 |
| lectin histochemical study on the reproductive tract in normal and brucella ovis-infected rams. | histochemical studies on tissue sections showed alterations of lectin-binding reactivities in the epididymis, seminal vesicle and ampulla of brucella ovis-infected rams. these modifications in the carbohydrate composition of organs participating in maturation, transport, and storage of spermatozoa, could be involved in the impaired fertility observed in this disease. | 1995 | 8593306 |
| isotype profiles induced in balb/c mice during foot and mouth disease (fmd) virus infection or immunization with different fmd vaccine formulations. | the igg isotype response in balb/c mice infected with fmdv or immunized with different vaccine formulations using inactivated virus particles as antigen was analyzed at various times post-inoculation. for this purpose an elisa based on polyclonal antibodies for detection and quantification of mouse igg isotypes with fmd virus (fmdv) specificity was developed. three immunomodulators, which have been shown to be very effective in inducing strong and long-lasting antibody responses (bahnemann, arch ... | 1995 | 7483770 |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| outer-membrane protein- and rough lipopolysaccharide-specific monoclonal antibodies protect mice against brucella ovis. | brucella ovis, a naturally virulent rough brucella species, is the aetiological agent of ram epididymitis. the identification of protective antigens is necessary to obtain a safe, specific subcellular vaccine. monoclonal antibodies (mabs) directed at both brucella outer-membrane proteins (omps) and rough lipopolysaccharide (r-lps) in a mouse protection test were used to identify potential targets for humoral immunity. mixtures of mabs directed at the 16.5-, 25-27-, 31-34- and 36-38-kda omps conf ... | 1995 | 7562998 |