Publications

identification of seoul hantavirus in rattus norvegicus in indonesia.	the first genetic evidence for the presence of seoul hantavirus (seov) in indonesia is presented. partial m segment sequence was recovered from the lung tissue of rattus norvegicus trapped in central jakarta. the sequence belongs to seov genotype and is most closely related to the strain b-1 from japan.	2004	15287380
seoul hantavirus in europe: first demonstration of the virus genome in wild rattus norvegicus captured in france.	although rats (rattus rattus or rattus norvegicus) worldwide have been found to carry seoul hantavirus, there are at present only a very few reports of confirmed human seoul hantavirus infections outside asia, where the virus, in certain areas, is responsible for approximately 25% of the human hantavirus infections. in europe, no confirmed human infections outside laboratories have been described, and although rats occasionally have been found to be antibody positive, the viral genome has not be ...	2004	15322934
epizootiological and epidemiological study of hantavirus infection in japan.	epizootiological surveys on hantavirus infections in rodents were carried out in various areas of japan, including the four major islands of hokkaido, honshu, shikoku, and kyushu from 2000 to 2003. a total of 1,221 rodents and insectivores were captured. seropositive animals were found in apodemus (a.) speciosus (5/482, 1.0%), rattus (r.) norvegicus (4/364, 1.1%), r. rattus (3/45, 6.7%), and clethrionomys (c.) rufocanus (7/197, 3.6%). the partial s segment was amplified from one seropositive r. ...	2004	15557742
identification of dobrava, hantaan, seoul, and puumala viruses by one-step real-time rt-pcr.	we developed four assays for specifically identifying dobrava (dob), hantaan (htn), puumala (puu), and seoul (seo) viruses. the assays are based on the real-time one-step reverse transcriptase polymerase chain reaction (rt-pcr) with the small segment used as the target sequence. the detection limits of dob, htn, puu, and seo assays were 25, 25, 25, and 12.5 plaque-forming units, respectively. the assays were evaluated in blinded experiments, each with 100 samples that contained andes, black cree ...	2004	15664046
microarray technology for identification and distinction of hantaviruses.	dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ...	2004	14981768
wounding: the primary mode of seoul virus transmission among male norway rats.	in rodent populations, males are more likely to be infected with hantaviruses and to engage in aggression than are females. to assess the relationship between aggression and seoul virus infection, norway rats were trapped in baltimore, maryland and wounding, infection status, and steroid hormone concentrations were examined. older males and males with high-grade wounds were more likely to have igg antibody to seoul, to shed virus in saliva, urine, and feces, and to have viral rna in organs than ...	2004	15031523
[seroprevalence of antibodies to seoul virus in occupational risk groups].	a seroprevalence study was performed to determine the percentage of people exposed to seoul virus among biologists and police officers (n = 226), whose work might put them into contact with viral reservoirs. detection of specific igg antibodies was performed by indirect immunofluorescence and western-blot. there was one positive result among the biologists (4.54%). this study confirms seoul virus exposure in one of the employment groups studied.	2004	15056437
nucleocapsid protein of cell culture-adapted seoul virus strain 80-39: analysis of its encoding sequence, expression in yeast and immuno-reactivity.	seoul virus (seov) is a hantavirus causing a mild to moderate form of hemorrhagic fever with renal syndrome that is distributed mainly in asia. the nucleocapsid (n) protein-encoding sequence of seov (strain 80-39) was rt-pcr-amplified and cloned into a yeast expression vector containing a galactose-inducible promoter. a survey of the pattern of synonymous codon preferences for a total of 22 n protein-encoding hantavirus genes including 13 of seov strains revealed that there is minor variation in ...	2005	15744561
variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china from 1985 to 2000.	we studied variability of g1 gene of hantaviruses occurring in the hubei province, p.r. china. serum samples were collected from 229 patients with hemorrhagic fever with renal syndromes (hfrs) during 1985--1989 and 1996--2000 and were tested by rt-pcr for the presence of hantaan and seoul viruses (htnvs, seovs) and by restriction fragment length polymorphism (rflp) analysis for the respective pattern. out of 229 sera 166 (72.5%) were hantavirus-positive by rt-pcr, including 124 from 1985--1989 a ...	2005	15929399
norway rat population in baltimore, maryland, 2004.	norway rats are reservoirs for several zoonotic agents, including hantaviruses, and are implicated in the transmission of pathogens to humans in urban environments. the rat population of baltimore, maryland was estimated from surveys in 1949 and again in 1952, but has not been evaluated for more than 50 years. previously identified sociodemographic risk factors for rat infestation, including median income, human density, and percentage of rental properties, were used to categorize census block g ...	2005	16187901
a pilot study for serological evidence of hantavirus infection in human population in south india.	hantaviruses are rodent-borne viruses of the family bunyaviridae that have been identified as aetiological agents of two human diseases, haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). there are no reports of hantavirus infections in humans from india, hence this pilot study was undertaken to provide the serological evidence of hantavirus infections in humans in south india.	2005	16251777
[genetics subtypes and distribution of seoul virus in henan].	to investigate the sub-genotypes and distribution ot seoul virus in henan.	2005	16390005
host factors mediating sex differences in viral infection.		2005	16464732
characterization of truncated hantavirus nucleocapsid proteins and their application for serotyping.	hemorrhagic fever with renal syndrome (hfrs) is a fulminant infectious disease characterized by fever, hemorrhage, renal impairment, and thrombocytopenia. hantaviruses associated with this belong to different serotypes: hantaan (htn), seoul (seo), dobrava/belgrade (dob), and puumala (puu). the first two, htn and seo, are endemic in china. to investigate the epidemiology of hfrs and virus transmission in china, we constructed prokaryotic plasmids encoding truncated recombinant htn and seo nucleoc ...	2006	16721853
[investigation on hantaviruses infection in rodents from free markets in beijing areas].	in order to find out the factors related to hemorrhagic fever with renal syndrome (hfrs) infection, and to evaluate the probability of ecdemic hantaviruses (hv) infection in rodents in beijing areas.	2006	16749997
[the pathogenetic role of peripheral t-lymphocytes activation-induced cell death in hemorrhagic fever with renal syndrome].	to study the activation-induced cell death (aicd) in peripheral blood t-lymphocytes (pbl-t) from patients of hemorrhagic fever with renal syndrome (hfrs) and the effect of aicd on the pathogenesis of hfrs.	2006	17313872
social status does not predict responses to seoul virus infection or reproductive success among male norway rats.	trade-offs exist among life history strategies that are used to increase survival and reproduction; such that, males that engage in more competitive behaviors may be more susceptible to infection. hantaviruses are transmitted horizontally between rodents through the passage of virus in saliva during wounding and male rodents are more likely to be infected with hantaviruses than females. to determine whether a trade-off exists between dominance and susceptibility to seoul virus infection, male lo ...	2006	16040226
genetic analysis of thailand hantavirus in bandicota indica trapped in thailand.	sixty one tissue samples from several rodent species trapped in five provinces of thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. four samples, all from the great bandicoot rat bandicota indica, were confirmed positive for the hantaviral n-antigen. two of them were trapped in nakhon pathom province, the other two in nakhon ratchasima province, approximately 250 km from the other trapping site. when analysed by rt-nested pcr, all four rodent ...	2006	16953877
regions of importance for interaction of puumala virus nucleocapsid subunits.	puumala virus (puuv) is a hantavirus that causes a mild form of hemorrhagic fever with renal syndrome in northern and central europe, and in large parts of russia. the nucleocapsid (n) protein encoded by hantaviruses plays an important role in the life-cycle of these viruses, and one important function for the n-protein is to oligomerize, surround and protect the viral rnas. we have identified amino- and carboxy-terminal regions involved in puuv n-n interactions, which comprise amino acids 100-1 ...	2006	16972031
[isolation and characterization of hantavirus carried by rodents in huludao, liaoning province].	to investigate the hantavirus infection and their genotype in rodents in huludao.	2006	17152513
a survey of zoonotic pathogens carried by norway rats in baltimore, maryland, usa.	norway rats (rattus norvegicus) carry several zoonotic pathogens and because rats and humans live in close proximity in urban environments, there exists potential for transmission. to identify zoonotic agents carried by rats in baltimore, maryland, usa, we live-trapped 201 rats during 2005-2006 and screened them for a panel of viruses, bacteria, and parasites. antibodies against seoul virus (57.7%), hepatitis e virus (hev, 73.5%), leptospira interrogans (65.3%), bartonella elizabethae (34.1%), a ...	2007	17224086
cross-reactive immune responses in mice after genetic vaccination with cdna encoding hantavirus nucleocapsid proteins.	hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in about 150,000 individuals in eurasia, and several hundred cases of hantavirus pulmonary syndrome (hps) on the american continent annually. there is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. in this study we have performed dna-vaccination of mice with full-length genes encoding the immunogenic nucleocapsid protein (np) of puumala (puuv), seoul (seov) and sin nombre virus (snv). th ...	2007	17110000
[virological surveillance on hemorrhagic fever with renal syndrome in hunan province in 2006].	to understand the rate of viral carrying status among rodents as well as genotypes and distribution of hantaviruses (hv) isolated in hunan province.	2007	18476580
regulatory t cells enhance persistence of the zoonotic pathogen seoul virus in its reservoir host.	hantaviruses are zoonotic pathogens that maintain a persistent infection in their reservoir hosts, yet the mechanisms mediating persistence remain unknown. regulatory t cell responses cause persistent infection by suppressing proinflammatory and effector t cell activity; hantaviruses may exploit these responses to cause persistence. to test this hypothesis, male norway rats were inoculated with seoul virus and regulatory t cells were monitored during infection. increased numbers of cd4(+)cd25(+) ...	2007	17878294
sequence analysis of the m genome segment of two seoul virus isolates from shandong province, china.		2007	17432948
nucleotide sequence characterization and phylogenetic analysis of hantaviruses isolated in shandong province, china.	china is the most severe endemic area of hemorrhagic fever with renal syndrome (hfrs) in the world with 30,000-50,000 cases reported annually, which accounts for more than 90% of total number of cases worldwide. the incidence rate of the syndrome in shandong province is one of the highest in china, which has ever reached 50 per 100,000 persons per year. however, the molecular characteristics of hantaviruses (hv) epidemic in shandong province remain unclear. therefore it is useful to clarify nucl ...	2007	17531126
[an epidemiologle investigation of hantaviruses carried by rodent hosts in hunan province].	to explore the hantavirus infection and their genotype in rodents in hunan.	2007	17575936
envelope glycoproteins of hantavirus can mediate cell-cell fusion independently.	hantaviruses (htvs) are enveloped viruses and can induce low ph-dependent cell fusion. in this report we molecularly cloned viral glycoproteins (gps) cdna and nucleocapsid (np) cdna of two strains of hantaan virus and one strain of seoul virus and expressed in vero e6 cells under control of a cmv promoter. the examinations of viral gene expressions were carried out by ifa and immune-precipitation. after treatment with low ph (ph 5.8) medium the syncytium were observed in the cells transfected wi ...	2007	17619252
detection and typing of human pathogenic hantaviruses by real-time reverse transcription-pcr and pyrosequencing.	because the clinical course of human infections with hantaviruses can vary from subclinical to fatal, rapid and reliable detection of hantaviruses is essential. to date, the diagnosis of hantavirus infection is based mainly on serologic assays, and the detection of hantaviral rna by the commonly used reverse transcription (rt)-pcr is difficult because of high sequence diversity of hantaviruses and low viral loads in clinical specimens.	2007	17717126
elevated testosterone and reduced 5-hiaa concentrations are associated with wounding and hantavirus infection in male norway rats.	among rodents that carry hantaviruses, males are more likely to engage in aggression and to be infected than females. one mode of hantavirus transmission is via the passage of virus in saliva during wounding. the extent to which hantaviruses cause physiological changes in their rodent host that increase aggression and, therefore, virus transmission has not been fully documented. to assess whether steroid hormones and neurotransmitters contribute to the correlation between aggression and seoul vi ...	2007	17719050
genetic properties of medium (m) and small (s) genomic rna segments of seoul hantavirus isolated from rattus norvegicus and antigenicity analysis of recombinant nucleocapsid protein.	a novel isolate of seoul (seo) hantaviruses was detected and identified in rattus norvegicus in shandong province, china and designated as jun5-14. the partial m segment and the coding region of nucleocapsid protein (np) in the s segment of jun5-14 were pcr-amplified and sequenced. nucleotide sequence analysis of the partial m segment (300 bp) revealed that jun5-14 isolate was closely related to former seo isolates in shandong (97.3-99.0% homology) and non-shandong seo viruses (84.1-97.7% homolo ...	2007	16927115
a soluble and heat-resistant form of hantavirus nucleocapsid protein for the serodiagnosis of hfrs.	hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne viral zoonosis characterized by fever, hemorrhagic manifestations, and renal disorder. the causative agent of hfrs has been identified as a hantavirus. hantavirus nucleocapsid proteins have recently been shown to be immunodominant antigens in hfrs, inducing an early and long-lasting immune response, and their amino termini are sensitive tools for the detection of specific antibodies in hfrs patient sera. previous work has demonstrate ...	2008	17826847
sex differences in the recognition of and innate antiviral responses to seoul virus in norway rats.	among rodents that carry hantaviruses, more males are infected than females. male rats also have elevated copies of seoul virus rna and reduced transcription of immune-related genes in the lungs than females. to further characterize sex differences in antiviral defenses and whether these differences are mediated by gonadal hormones, we examined viral rna in the lungs, virus shedding in saliva, and antiviral defenses among male and female rats that were intact, gonadectomized neonatally, or gonad ...	2008	18053684
new and old world hantaviruses differentially utilize host cytoskeletal components during their life cycles.	recently we reported that the n protein of the old world hantavirus, hantaan (htnv), traffics on microtubules to the er-golgi intermediate compartment (ergic) prior to its movement to the golgi and requires an intact ergic for viral replication. we have extended these studies to the new world hantaviruses, andes virus (andv) and black creek canal virus (bccv), and an additional old world hantavirus, seoul virus (seov). these studies support microtubule-dependent trafficking of the n protein to e ...	2008	18234268
development of recombinant canine adenovirus type-2 expressing the gn glycoprotein of seoul virus.	seoul virus glycoprotein gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. in this study, a replication-competent recombinant canine adenovirus type-2 expressing gn was constructed by the in vitro ligation method. the gn expression cassette, including the human cytomegalovirus (hcmv) promoter/enhancer and the sv40 early mrna polyadenylation signal, was cloned into the sspi site of the e3 region which is not ess ...	2008	18249007
prevalence of hantavirus infection in wild rattus norvegicus and r. rattus populations of buenos aires city, argentina.	to determine the prevalence of infection with hantaviruses in wild rattus norvegicus and r. rattus populations in areas of buenos aires city.	2008	18291001
[molecular characterization of hantavirus zhejiang isolate zt10 strain from m. fartis].	to learn about the complete genomic sequence of the seoul virus strain zt10 isolated from m. fartis.	2008	18414685
genetic characterization of hantaviruses isolated from guizhou, china: evidence for spillover and reassortment in nature.	to gain more insights into the epidemiology of hantaviruses in the guizhou province, china, rodents were captured in guizhou during the period from 2001 to 2003. in addition, serum sample was collected from one patient. virus isolation was attempted from human serum and rodent samples. four hantaviruses were isolated successfully in cell culture from one human, two a. agrarius, and one r. norvegicus. the nucleotide sequences for the entire s and m and partial l segment were determined from these ...	2008	18428127
seoul virus in patients and rodents from beijing, china.	hemorrhagic fever with renal syndrome (hfrs) is a significant public health problem with an increasing incidence in beijing, china (report of disease surveillance from the center for disease control and prevention of beijing, china). hantaviruses were detected using rt-pcr method in blood samples of hfrs patients and lung tissues of rodents captured in beijing. phylogenetic analyses of 724bp partial s segment of the hantavirus gene showed that the detected seoul virus (seov) fell into three diff ...	2008	18458321
seoul virus enhances regulatory and reduces proinflammatory responses in male norway rats.	zoonotic pathogens, including hantaviruses, are maintained in the environment by causing persistent infection in the absence of disease in their reservoir hosts. spillover of hantaviruses to humans can cause severe disease that is mediated by excessive proinflammatory responses. the mechanisms mediating hantaviral persistence in rodent reservoirs remain largely unknown. male norway rats were inoculated with their species-specific hantavirus, seoul virus (seov), and viral rna, cytokine, and chemo ...	2008	18461618
development of a serotyping elisa system for thailand virus infection.	to distinguish thailand virus infection from infections with other hantaviruses, we established an elisa serotyping system using a truncated nucleocapsid protein of thailand virus lacking 49 amino acids at the n-terminus. in evaluations using patient and rodent sera, thailand virus infection was readily distinguished from hantaan and seoul virus infections. therefore, this elisa system is an effective alternative to neutralization tests.	2008	18551243
[related substitutions in the small segment of old world hantavirus genome].	whether there were related nucleotide substitutions in the s-segments of the genome of tula, puumala, hantaan, dorava/belgrad, and seoul viruses was studied. the significance of the correlations found in nucleotide substitutions was estimated by the level of mutual information. the majority of the correlated changes were synonymous substitutions. the correlations were mostly restricted to non-synonymous substitutions sites: tula 258 (a.a.) and dobrava/belgrad (56 a.a.). in the genome of puumala, ...	2008	18590133
cross-reactive and serospecific epitopes of nucleocapsid proteins of three hantaviruses: prospects for new diagnostic tools.	the diagnosis of infectious diseases is sometimes difficult because of extensive immunological cross-reactivity between related viral antigens. on the path of constructing sero-specific antigens, we have identified residues involved in sero-specific and cross-reactive recognition of the nucleocapsid proteins (nps) of puumala virus (puuv), seoul virus (seov), and sin nombre virus (snv) using serum samples from 17 nephropathia epidemica patients. the mapping was performed by enzyme-linked immunoso ...	2008	18620010
[zoonoses transmitted by mouse and rat maintained as laboratory or pet animals].	large numbers of mice (mus spp.) and rats (rattus spp.) are maintained for scientific reasons and as pet animals in germany. while laboratory animals are monitored for pathogenic agents, the hygienic status of pet animals is usually completely unknown. despite great efforts, zoonotic infections were reported even in laboratory settings, e.g. with hantavirus (seoul virus), streptobacillus moniliformis, and trichphyton mentagrophytes. however, in current reports, zoonotic infections were transmitt ...	2008	18712260
[study on the molecular epidemiology of hantavirus carried by rodent hosts in cixi, zhejiang province].	to collect more data on the epidemiology of hantavirus in rodents in cixi, zhejiang province.	2008	18843995
[study on the molecular characteristic of natural infection of rodents with hantaviruses in shenzhen city].	in order to investigate hantavirus (hv) infection of captured rodents and to understand the genotypes and the molecular characteristic of hantaviruses in shenzhen.	2008	18844081
corticosteroids modulate seoul virus infection, regulatory t-cell responses and matrix metalloprotease 9 expression in male, but not female, norway rats.	human hantaviral disease is mediated by excessive proinflammatory and cd8+ t-cell responses, which can be alleviated by administration of corticosteroids. in contrast with humans, male rats that are infected with their species-specific hantavirus, seoul virus (seov), have reduced proinflammatory and elevated regulatory t-cell responses in tissues where virus persists. to determine the effects of glucocorticoids on seov persistence and immune responses during infection, male and female norway rat ...	2008	18931068
[the molecular epidemiologic investigation of hantavirus carried by rodent hosts in wenzhou, zhejiang province].	to study the epidemiological features of hantavirus in rodents in wenzhou, zhejiang province.	2008	19173853
hantaviruses: an emerging public health threat in india? a review.	the emerging viral diseases haemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps) are a cause of global concern as they are increasingly reported from newer regions of the world. the hantavirus species causing hfrs include hantaan virus,seoul virus, puumala virus, and dobrava-belgrade virus while sin nombre virus was responsible for the 1993 outbreak of hcps in the four corners region of the us. humans are accidental hosts and get infected by aerosols gener ...	2008	19208975
[molecular characterization and sequence comparison of the m and s segments of hantavirus zj5 strain].	in order to understand the molecular characters of hantavirus zj5 strain, its complete m and s genome were sequenced and compared with that of other hantavirus strains.	2008	19544637
a single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus gn glycoprotein confers protective immunity against seoul virus in mice.	seoul virus (seov), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) and afflicts tens of thousands of people annually. in this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gn protein of seov (rcav-2-gn) in balb/c mice. sera from immunized mice contained neutralizing antibodies that could specifically recognize seov and neutralize its infectivity in vitro. ...	2009	19583957
[survey on host animal and molecular epidemiology of hantavirus in chuxiong prefecture, yunnan province].	to determine the hosts of hantavirus (hv) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (hfrs).	2009	19642377
[development and evaluation of taqman-based one-step reverse transcription-polymerase chain reaction assay for the detection of japanese encephalitis virus].	to establish a taqman based real-time reverse transcription-polymerase chain reaction (rt-pcr) assay for the detection of japanese encephalitis virus.	2009	19642386
genetic characterization of a juquitiba-like viral lineage in oligoryzomys nigripes in rio de janeiro, brazil.	hantaviruses, family bunyaviridae, are rodent-borne rna viruses that have caused cases of hantavirus cardiopulmonary syndrome (hcps) in various regions of the americas. there are five hantaviral lineages associated with hcps in brazil: juquitiba virus (juqv), araraquara virus (arav), laguna negra-like virus (lnv), castelo dos sonhos virus (casv), and anajatuba virus (anajv). three additional hantaviruses have been described in rodents alone: rio mearim virus, jaborá virus, and a hantavirus linea ...	2009	19660427
domestically acquired seoul virus causing hemorrhagic fever with renal syndrome-maryland, 2008.	hantaviruses are rodent-borne viruses capable of causing human disease. the seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome in east asia. to our knowledge, we report the first domestically acquired case of hemorrhagic fever with renal syndrome caused by the seoul virus, confirmed by serology testing, reverse-transcriptase polymerase chain reaction, and nucleotide sequence analysis. the patient presented with myalgias and fever, and developed acute renal failure.	2009	19848600
co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary.	hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ...	2009	19856478
molecular epidemiological and serological studies of hantavirus infection in northern vietnam.	the distribution of anti-hantavirus antibodies in humans and rodents in northern vietnam was examined. in total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for igg antibodies against hantaan or seoul virus (seov) by elisa, ifa, and western blot analysis. antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of hai phong, and 2/185 residents of ha nam province. in comparison ...	2009	19887743
hantaviruses in rodents and humans, inner mongolia autonomous region, china.	surveys were carried out in 2003-2006 to better understand the epidemiology of hantaviruses in the inner mongolia autonomous region of china (inner mongolia). hemorrhagic fever with renal syndrome (hfrs) was first reported in this region in 1955 and has been an important public health problem here since then. during 1955-2006, 8,309 persons with hfrs were reported in inner mongolia (average incidence rate 0.89/100,000), and 261 (3.14%) died. before the 1990s, all hfrs cases occurred in northeast ...	2009	19523286
genotyping of hantaviruses occurring in linyi, china, by nested rt-pcr combined with single-strand conformation polymorphism analysis.	the hemorrhagic fever with renal syndrome (hfrs) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. in this study, 48 serum samples were collected from patients with hfrs in linyi area, china, that is seriously affected by this disease. the collected samples were analyzed by nested rt-pcr combined with single-strand conformation polymorphism (sscp) for geno ...	2009	19537913
seoul virus and hantavirus disease, shenyang, people's republic of china.	an outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred among students in shenyang pharmaceutical university in 2006. we conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals. immunoglobulin (ig) m or igg antibodies against seoul virus (seov) were detected in the serum samples of all 8 patients. igg antibodies against hantavirus were also identified in laboratory rats, which were ...	2009	19193263
a newly recognized hantavirus in the asian house rat (rattus tanezumi) in indonesia.	hantaviral sequences were recovered from the lung tissue of an asian house rat (rattus tanezumi) captured in serang, indonesia. phylogenetic analysis of partial l, m and s segment sequences showed that they belonged to a novel hantavirus provisionally named serang virus (serv). notably, serv is distinct from the hantaviruses associated with rodents of the species rattus: seoul virus associated with rattus norvegicus worldwide and gou virus isolated from rattus rattus in china. instead serv appea ...	2009	19088290
serological and genetic evidence for the presence of seoul hantavirus in rattus norvegicus in flanders, belgium.	seoul hantavirus (seov), carried by rattus rattus (black rat) and r. norvegicus (norway, brown rat), was reported to circulate as well as cause hfrs cases in asia. as rattus sp. are present worldwide, seov has the potential to cause human disease worldwide. in europe however, only seov prevalence in rats from france was reported and no confirmed cases of seov infection were published. we here report genetic and serological evidence for the presence of seov virus in brown rat populations in belgi ...	2009	18821445
[comparison on the difference of virulence between hantaan virus and seoul virus isolated both from rattus norvegicus].	comparing the difference of virulence between the strain cgrn5310 (htnv) and the strain hr54 (seov) isolated both from rattus norvegicus.	2009	20193387
[association of hemorrhagic fever with renal syndrome and hla-drb allele polymorphisms in han nationality, hubei province.].	to understand the association of human leukocyte antigen (hla)-drb polymorphism and patients diagnosed as hemorrhagic fever with renal syndrome (hfrs).	2009	20193583
hantaviruses in small mammals and humans in the coastal region of zhejiang province, china.	to gain more insights into the epidemiology of hantaviruses in the coastal region of zhejiang province, china, the morbidity and mortality of hemorrhagic fever with renal syndrome (hfrs) were analyzed in two coastal areas: cixi (hilly terrain) and wenzhou (mountainous terrain). more hfrs cases have been reported in cixi than in wenzhou. annual incidence rate of hfrs in cixi had been on the level of approximately 1.5/100,000 from 1968 (when the first hfrs case was reported) to 2007, with the high ...	2010	20419812
hantavirus infections in humans and animals, china.	hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in the people's republic of china. although 7 sero/genotypes of hantaviruses have been found in rodents, only hantaan virus (carried by apodemus agrarius mice) and seoul virus (carried by rattus norvegicus rats) reportedly cause disease in humans. during 1950-2007, a total of 1,557,622 cases of hfrs in humans and 46,427 deaths (3%) were reported in china. hfrs has been reported in 29 of 31 provinces in china. after i ...	2010	20678311
spatiotemporal trends and climatic factors of hemorrhagic fever with renal syndrome epidemic in shandong province, china.	hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne disease caused by hantaviruses. it is endemic in all 31 provinces, autonomous regions, and metropolitan areas in mainland china where human cases account for 90% of the total global cases. shandong province is among the most serious endemic areas. hfrs cases in shandong province were first reported in yutai county in 1968. since then, the disease has spread across the province, and as of 2005, all 111 counties were reported to have l ...	2010	20706629
tracking hantavirus nucleocapsid protein using intracellular antibodies.	hantavirus nucleocapsid (n) protein is a multifunctional viral macromolecule involved in multiple stages of the viral replication cycle. the intracellular trafficking of n protein during virus assembly remains unclear.	2010	21092325
seoul virus infection in a wisconsin patient with recent travel to china, march 2009: first documented case in the midwestern united states.	diagnosis of seoul virus-associated hemorrhagic fever with renal syndrome (hfrs) cases among united states residents is rare. we describe confirmation of a seoul virus infection in a 36-year-old scientist who worked with laboratory rats in milwaukee, wisconsin, but most likely acquired the infection during a trip to shenyang, china.	2010	21118933
evidence of human hantavirus infection and zoonotic investigation of hantavirus prevalence in rodents in western java, indonesia.	abstract during febrile surveillance in the western java city of bandung, indonesia, a patient with clinical symptoms consistent with hantavirus infection was found to have elevated titers of hantavirus-specific immunoglobulin m (igm) and igg antibodies. a subsequent epizoological investigation demonstrated a higher prevalence of hantavirus igg antibodies in rodents trapped in the vicinity of the patient's home compared with rodents from a control area (13.2% vs. 4.7%, p = 0.036). the old world ...	2010	21142967
[genetic analysis of hantaviruses carried by rattus norvegicus collected from henan and neimenggu provinces, china.]	objective: genetic analysis was performed to infer the relationship between hantaviruses carried by rattus norvegicus from henan and neimenggu provinces and the other known hantavirus and the vaccine strain. methods: total rna was extracted from lung tissues with trizol reagent. the complete m and s segment sequences of strains nm133 and q12 were amplified by rt-pcr. the purified dna fragments were directly subjected to sequencing, and then to sequence analysis and phylogenetic analysis. results ...	2010	21162871
[genetic characterization of two seoul virus strains isolated from patients and rats in hebei province.]	objective: complete s and m segments of two seoul virus (seov) strains were obtained to determine their genetic types and characteristics. methods: the complete s and m segments from the isolate li and lung tissue (sample lf18) were amplified by rt-pcr. genetic analyses were performed by using dnastar and phylip program package. results: their sequences consisted of 1772 nucleotides, and had an open reading frame (orf, 43 to 1332 nt) encoding a nucleoprotein of 429 amino acids for both two strai ...	2010	21162996
molecular characterization of two hantavirus strains from different rattus species in singapore.	hantaviruses cause human disease in endemic regions around the world. outbreaks of hantaviral diseases have been associated with changes in rodent population density and adaptation to human settlements leading to their proliferation in close proximity to human dwellings. in a parallel study initiated to determine the prevalence of pathogens in singapore's wild rodent population, 1206 rodents were trapped and screened. the findings established a hantavirus seroprevalence of 34%. this paper descri ...	2010	20096099
hemorrhagic fever with renal syndrome, vietnam.		2010	20113591
seoul virus suppresses nf-kappab-mediated inflammatory responses of antigen presenting cells from norway rats.	hantavirus infection reduces antiviral defenses, increases regulatory responses, and causes persistent infection in rodent hosts. to address whether hantaviruses alter the maturation and functional activity of antigen presenting cells (apcs), rat bone marrow-derived dendritic cells (bmdcs) and macrophages (bmdms) were generated and infected with seoul virus (seov) or stimulated with tlr ligands. seov infected both dcs and macrophages, but copies of viral rna, viral antigen, and infectious virus ...	2010	20170933
generation of e3-deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences.	seoul virus (seov) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. the medium genome segment encodes the gn/gc glycoproteins of seov, which form the major structural part of the virus envelope. gc and/or gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. in this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gc protein of seov w ...	2010	20181748
geo-spatial hotspots of hemorrhagic fever with renal syndrome and genetic characterization of seoul variants in beijing, china.	hemorrhagic fever with renal syndrome (hfrs) is highly endemic in mainland china, and has extended from rural areas to cities recently. beijing metropolis is a novel affected region, where the hfrs incidence seems to be diverse from place to place.	2011	21264354
serological evidence of thailand virus-related hantavirus infection among suspected leptospirosis patients in kandy, sri lanka.	a cross-sectional study was undertaken to determine the current prevalence of leptospirosis and hantaviral infections, and the socio-demographic characteristics and risk factors of infected patients, in kandy, sri lanka. this report discusses the serological evidence of hantavirus infections among 105 suspected leptospirosis patients, 8 of whom had hantavirus antibodies. serotyping elisa showed that these 8 patients had high optical density values for thailand virus. most of the sera showed that ...	2011	21266762
seroepidemiological survey of hantavirus infection in healthy people in vallès occidental, barcelona.	hantaviruses are the etiological agents of hemorrhagic fever with renal syndrome in europe and asia, and hantavirus pulmonary syndrome in america. approximately 150,000 cases are reported annually worldwide. in spain, some hantavirus infection cases have been described. besides, rodents that have been described as hantavirus reservoirs are present. the aim of the present study was to determinate the seroprevalence of hantavirus in humans in the northeast of spain.	2011	21417923
comparison of hantaan and seoul viral infections among patients with hemorrhagic fever with renal syndrome (hfrs) in heilongjiang, china.	hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in china, and is primarily caused by either the hantaan virus (htnv) or seoul virus (seov) strains. however, the causative hantavirus has only been definitively identified in a few hfrs cases, and detailed comparisons of patient data for the 2 strains are limited.	2011	21428852
prevalence of hantavirus infection in wild rodents from five provinces in korea, 2007.	hemorrhagic fever with renal syndrome (hfrs) is an infectious disease caused by hantaviruses of the family bunyaviridae. among them, hantaan virus (htnv) is most widely distributed in korea. the striped field mouse, apodemus agrarius, is the natural host of htnv in rural korea. we trapped 766 small mammals of three species (1 eothenomys regulus, 13 crocidura suaveolens, and 752 apodemus agrarius) in five provinces in korea from january to december 2007. we tested 542 rodent sera for htnv antibod ...	2011	21441196
comparison of innate immune responses to pathogenic and putative non-pathogenic hantaviruses in vitro.	hantaviruses are human pathogens that cause hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. the mechanisms accounting for the differences in virulence between pathogenic and non-pathogenic hantaviruses are not well known. we have examined the pathogenesis of different hantavirus groups by comparing the innate immune responses induced in the host cell following infection by pathogenic (sin nombre, hantaan, and seoul virus) and putative non-pathogenic (prospect hill, ...	2011	21820021
Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand.	In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detec ...	2011	22124701
Genetic analysis of hantaviruses and their rodent hosts in central-south China.	Hantaan virus (HTNV) and Seoul virus (SEOV) are two major zoonotic pathogens of hemorrhagic fever with renal syndrome (HFRS) in Asia. Hubei province, which is located in the central-south China, had been one of the most severe epidemic areas of HFRS. To investigate phylogenetic relationships, genetic diversity and geographic distribution of HTNV and SEOV in their reservoir hosts, a total of 687 rodents were trapped in this area between 2000 and 2009. Sequences of partial S- and M-segments of han ...	2011	22108583
application of truncated nucleocapsid protein (n) for serotyping elisa of murinae-associated hantavirus infection in rats.	truncated recombinant nucleocapsid proteins (trns) that lack n-terminally located cross-reactive epitopes of four murinae rodent-associated hantaviruses, seoul virus (seov), thailand virus, hantaan virus (htnv) and dobrava-berglade virus, were produced by using a baculovirus expression system. elisa with the trns as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses as cut-off od values of 60% to those to whole n of htnv. the trn ba ...	2011	21937858
identification of three novel ctl epitopes within nucleocapsid protein of hantaan virus.	hantaan virus (htnv) is a member of the hantavirus genus that causes human hemorrhagic fever with renal syndrome (hfrs) in humans. the ctl response seems to play a key role in control of viral infection, but only a few htnv epitopes recognized by the ctls have been reported. herein, we screened a panel of overlapping peptides covering the htnv nucleocapsid protein by elispot assays for those that can elicit ifn-γ production in vitro. three novel cd8(+) ctl epitopes, n197-205 (ryrtavcgl), n245-25 ...	2011	22111596
Severe pulmonary involvement in a case attributed to domestically acquired seoul hantavirus in the United States.	Hantavirus is known to cause 2 distinct clinical syndromes: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome. Seoul virus is an Old World hantavirus known to cause HFRS. We report a case attributed to domestically acquired Seoul hantavirus with prominent pulmonary involvement and a fatal outcome.	2012	22042876
induction of protective immunity in a syrian hamster model against a cytopathogenic strain of andes virus.	andes virus (andv) is responsible for the hantavirus pulmonary syndrome cases in argentina and neighboring countries, with moderate to high case-fatality rates. andv has some particular features, which make it unique among other members of the hantavirus genus such as person-to-person transmission and causing a disease similar to hantavirus pulmonary syndrome in the hamster as an animal model. the kinetics of replication in vero e6 cells of an andv strain isolated in argentina, called andes/arg, ...	2012	22095538
pathogenicity of a natural reassortant hantavirus cgrn9415 in newborn rats and newborn mice.	to better understand the pathogenicity and infectivity of a natural reassortant cgrn9415 generated from hantaan virus (htnv) and seoul virus (seov), cgrn9415, htnv 76-118, and seov l99 were used to infect newborn kunming (km) mice and newborn wistar rats. in km mice, there was no statistic difference between the death rate with cgrn9415 and that with l99, while 76-118 killed all mice even at low dosage; cgrn9415 killed all infected rats like l99 at the dosage of 105 ffu while no death occurred i ...	2012	22258864
Migration of norway rats resulted in the worldwide distribution of seoul hantavirus today.	Despite the worldwide distribution, most of the known Seoul viruses (SEOV) are closely related to each other. In this study, the M and the S segment sequences of SEOV were recovered from 130 lung tissue samples (mostly of Norway rats) and from six patient serum samples by reverse transcription-PCR. Genetic analysis revealed that all sequences belong to SEOV and represent 136 novel strains. Phylogenetic analysis of all available M and S segment sequences of SEOV, including 136 novel Chinese strai ...	2012	22090114
genetic characterization of seoul hantavirus originated from norway rats (rattus norvegicus) captured in belgium.	hantaviruses (genus hantavirus, family bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. so far, in europe, four pathogenic hantaviruses have been found, often in co-circulation: puumala virus (puuv), dobrava virus (dobv), saaremaa virus (saav), and seoul virus (seov). of those, only puuv was found in belgium. recently, in our search for hantaviruses in the flanders region of belgium we collected genetic ...	2012	22711359
immunogenicity of a multi-epitope dna vaccine against hantavirus.	hemorrhagic fever with renal syndrome (hfrs) is a severe epidemic disease caused by hantaviruses including hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus. three of the four hfrs hantaviruses, htnv, seov, and puuv are found in china. currently, there is no effective strategy available to reduce infection risk. in this study, we constructed a multi-epitope chimeric dna vaccine that encodes expressing 25 glycoprotein epitopes from seov, htnv and puuv (designated as ...	2012	22426376
[genetic characteristics of hantaviruses carried by microtus maximowixzii in yakeshi of inner mongolia, china].	to analyze the viral genetic characteristics of hantaviruses carried by microtus maximowixzii in yakeshi of inner mongolia autonomous region and its relationship with hantaan virus (htnv) and seoul virus (seov) viruses as well as to identify the natural host of khabarovsk virus (khav).	2012	22967339
dynamics of hantavirus infections in humans and animals in wuhan city, hubei, china.	hemorrhagic fever with renal syndrome (hfrs) has been a significant public problem since the first cases were reported in 1961 in wuhan city (capital of hubei province of china). epidemiological surveys were carried out to better understand the dynamics of hantavirus infection in humans and animals in wuhan. during 1961-2011, a total of 21,820 hfrs cases were registered in wuhan. the two large epidemics had occurred during 1970-1991. they reached peaks in 1973 and 1983, respectively. there have ...	2012	22910184
cross-species transmission in the speciation of the currently known murinae-associated hantaviruses.	to gain more insight into the phylogeny of dabieshan virus (dbsv), carried by niviventer confucianus and other murinae-associated hantaviruses, genome sequences of novel variants of dbsv were recovered from niviventer rats trapped in the mountainous areas of wenzhou, china. genetic analyses show that all known genetic variants of dbsv, including the ones identified in this study, are distinct from other murinae-associated hantaviruses. dbsv variants show geographic clustering and high intraspeci ...	2012	22855492
hantaviruses in rodents and humans, xi'an, pr china.	xi'an, the capital of shaanxi province, located in north-west china, is one of the major endemic areas for haemorrhagic fever with renal syndrome (hfrs). in this study, the epidemiological data of hfrs in xi'an from 1959 to 2010, especially in the past ten years (2001-2010), were surveyed. the features of hantavirus (hv) host carriers, the molecular characteristics of the hv s gene from hosts and patients, and the genome of the viral isolate were also investigated. data showed that there might b ...	2012	22837422
studies on hantavirus infection in small mammals captured in southern and central highland area of vietnam.	to investigate the distribution of hantaviruses among animals in southern and central highland area of vietnam, a total of 1311 serum samples were obtained from rats and asian house shrews (suncus murinus) captured at 11 locations between 2006 and 2009. a total of 1066 serum samples from rats were examined for igg antibodies against hantaan virus, and there were 30 antibody-positive serum samples from rats that had been captured mainly in a port area and urban area in ho chi minh city (hcmc) (2. ...	2012	22673720
isolation and characterization of hantaviruses in far east russia and etiology of hemorrhagic fever with renal syndrome in the region.	hemorrhagic fever with renal syndrome (hfrs) is a serious public health issue in far east russia. two different hantaviruses were isolated from rodents captured in the khabarovsk region: amur virus (amrv; khekhtsir/ap209/2005 strain from apodemus peninsulae) and hantaan virus (htnv; galkino/aa57/2002 strain from a. agrarius). genetic analysis of the new isolates revealed that the m and l segments were apparently different between amrv and htnv, but s segments of the two viruses were closer. the ...	2012	22403334
complete genome sequence of seoul virus isolated from rattus norvegicus in the democratic people's republic of korea.	seoul virus (seov) is responsible for 25% of cases of hemorrhagic fever with renal syndrome in asia. here we report the complete genome of strain dprk08. the sequence information provided here is useful for understanding the molecular character of seov in the democratic people's republic of korea (dprk) and the circulation of seov in east asia.	2012	23166256
[genetic analysis on s segment of hantaviruses in rodent hosts from wuhan area, hubei province].	to investigate the infection and genotype of hantaviruses in rodents from wuhan area, hubei province.	2012	22967338