Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cytomegalovirus-infected cell polypeptides immune-precipitated by sera from children with congenital and perinatal infections. | congenital or perinatally acquired human cytomegalovirus (cmv) infections in children may be symptomatic or asymptomatic. in this study, we characterized the electrophoretic properties of cmv-infected cell polypeptides immune-precipitated by sera from children with different types of cmv infections from birth to 4 years of age. sodium dodecyl sulfate-polyacrylamide gel analysis of immune precipitates formed with radiolabeled extracts of cells infected with cmv strain ad169 showed the following. ... | 1983 | 6295952 |
| influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus dna. | the efficiency of transfection of purified hcmv genome was studied in synchronized human embryo fibroblasts. the data show that hcmv dna infection performed in s phase is more efficient than in cells infected in g2, m and g1 phases of cell cycle, respectively. these findings stress the importance of the metabolic state of cell cultures in hcmv genome transfection. | 1983 | 6318698 |
| [production of monoclonal antibodies against human cytomegalovirus]. | cell fusion of sp2/0, a murine myeloma derived non-secreting variant, with splenic lymphocytes from mice immunized against human cytomegalovirus, has originated two stable hybridomas producing monoclonal antibodies (igg) which react specifically with antigens that appear early in infectious cycle and remain localized in the cell nucleus. | 1983 | 6318932 |
| current problems in human cytomegalovirus diagnosis. | 1983 | 6319319 | |
| organization and expression of the immediate early genes of human cytomegalovirus. | the immediate early genes of human cytomegalovirus were characterized according to map location, rna transcripts, and translation products. three regions in the large unique component (0.709 to 0.751 map units) were transcribed in the presence of an inhibitor of protein synthesis (anisomycin). a single size class of polyadenylated mrna, 1.95 kilobases (kb), was transcribed abundantly relative to the other size classes. the predominant 1.95-kb viral rna was transcribed from right to left on the p ... | 1983 | 6298447 |
| supernatant virus release as a differentiating marker between low passage and vaccine strains of human cytomegalovirus. | the ratio of cmv virus released into the supernatant per infected human fibroblast was studied for three laboratory strains and seven fresh isolates. the former all gave ratios over one whereas the latter gave ratios less than one. this phenomenon may serve as a marker of high passage attenuated virus, as about 25 passages were required in case of one strain to change this property. | 1983 | 6099639 |
| replicative forms of human cytomegalovirus dna with joined termini are found in permissively infected human cells but not in non-permissive balb/c-3t3 mouse cells. | 1983 | 6300290 | |
| rapid detection and quantitation of human cytomegalovirus in urine through dna hybridization. | we detected cytomegalovirus dna in clinical urine specimens after immobilization on nitrocellulose filters and hybridization with a radioactively labeled, cloned fragment of cytomegalovirus dna. we accomplished the specific detection and quantitation of viral dna within 24 hours with 39 urine specimens from nine patients with cytomegalovirus viruria, mostly at a tissue-culture infective titer of 10(3) per milliliter or higher. none of 57 urine specimens from 21 patients that were culture-negativ ... | 1983 | 6300675 |
| correlation between stimulation of host cell dna synthesis by human cytomegalovirus and lack of expression of a subset of early virus genes. | the stimulation of host cell dna synthesis was studied in permissive human embryonic lung (hel) cells and in nonpermissive rabbit kidney (rk) cells infected with human cytomegalovirus (hcmv). host cell dna synthesis was induced by hcmv infection in resting cells of both types. in permissive cultures the stimulation of cellular dna synthesis was detectable mainly in those cells which had not become productively infected and in which virus antigens were not detectable. in abortively infected rk ce ... | 1983 | 6312675 |
| the use of acyclovir for cytomegalovirus infections in the immunocompromised host. | despite the lack of virus-specified thymidine kinase activity, human cytomegalovirus may be sensitive to acyclovir in vitro at concentrations between 10 and 25 mg/l. the inhibitory effect of acyclovir can be further increased by the presence of small amounts of human alpha or beta interferon. twenty-one allogeneic marrow graft recipients with biopsy-proven cytomegalovirus pneumonia were treated with either high doses of acyclovir (eight patients) or the combination of acyclovir and human alpha ( ... | 1983 | 6313597 |
| efficacy and selectivity of some nucleoside analogs as anti-human cytomegalovirus agents. | 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluridine (fmau), 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouridine (fiau), and 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-ethyluridine (feau) were evaluated for antiviral activities against human cytomegalovirus (hcmv) and compared with 9-[(2-hydroxyethoxy)methyl]guanine (acyclovir) and e-5-(2'-bromovinyl)-2'-deoxyuridine (bvdu). the relative anti-hcmv pote ... | 1983 | 6316843 |
| structure of the transforming region of human cytomegalovirus ad169. | the minimum size fragment of human cytomegalovirus ad169 required to initiate transformation was determined by transfection of primary rat embryo cells with deletion fragments constructed by digestion of a cloned fragment containing the transforming region (pcm4000) with exonuclease iii and s1 nuclease. the results indicate that the left-hand boundary of the minimum size sequence for transformation must reside between 490 and 318 bases from the hindiii site of pcm4000. the right-hand boundary wa ... | 1984 | 6317885 |
| effects of certain nucleoside analogues on human cytomegalovirus replication in vitro. | four nucleoside analogues, 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), -5-iodouracil (fiau), -5-methylcytosine (fmac) and -5-iodocytosine (fiac), were studied for their effect on human cytomegalovirus (hcmv) replication in vitro. fmau, fiau, fmac and fiac showed antiviral activities for four strains of hcmv (major, clegg, d550 and towne) in a plaque reduction assay, with a dose required for 50% inhibition (ed50) in the range of 0.1 to 0.65 microm. at a concentration of ... | 1984 | 6319573 |
| predominant immediate-early transcripts of human cytomegalovirus ad 169. | transcription of the human cytomegalovirus genome (strain ad 169) was investigated at the immediate-early (ie) time after infection, by using cycloheximide to suppress virus-specific protein synthesis. in total cell rna, four predominant ie transcripts were found which were encoded by one contiguous region of the long unique segment between map units 0.06 and 0.16 in prototype arrangement of human cytomegalovirus ad 169 dna. analysis by northern blot hybridizations demonstrated that the transcri ... | 1984 | 6319737 |
| the oncogenic potential of human cytomegalovirus. | 1984 | 6322235 | |
| characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins. | four classes of monoclonal antibody-producing cell lines have been obtained that detect human cytomegalovirus virion structural proteins. these antibodies react with (1) a major outer membrane virion glycoprotein(s) gp58-gp130, whose molecular weight varies between strains of cytomegalovirus, (2) a phosphoprotein, pp71, localized inside the virion membrane, (3) a phosphorylated nucleocapsid protein, pp155, and (4) a virion-associated phosphoprotein, pp29. polyclonal immune human sera react with ... | 1984 | 6322416 |
| the proteins of human cytomegalovirus. | 1984 | 6329373 | |
| lymphocyte subsets and urinary excretion of cytomegalovirus among homosexual men attending a clinic for sexually transmitted diseases. | a significantly higher frequency of urinary excretion of human cytomegalovirus (hcmv) was noted in homosexual men (29 [18%] of 161) than in heterosexual men (3 [4%] of 77) attending a clinic for sexually transmitted diseases. however, differences were not significant when only persons with antibody to hcmv were compared (29 of 157 vs. 3 of 33). the homosexual men who excreted hcmv had a significantly lower mean ratio of t-helper (okt4+) to t-suppressor (okt8+) cells (1.13 +/- 0.09) than did the ... | 1984 | 6090540 |
| complementation of an adenovirus 5 immediate early mutant by human cytomegalovirus. | the initiation of the lytic cycle of adenovirus 5 requires synthesis of a transcriptional activator encoded by the viral early genetic unit, e1a. mutant viruses lacking e1a are defective. human cytomegalovirus activated transcription of early adenovirus genes and complemented an e1a- adenovirus mutant in cells permissive for both viruses. | 1984 | 6091338 |
| characterization of the guinea pig cytomegalovirus genome by molecular cloning and physical mapping. | fragments of guinea pig cytomegalovirus (gpcmv) dna produced by hindiii or ecori restriction endonuclease digestion were cloned into vectors pbr322 and pacyc184, and recombinant fragments representing ca. 97% of the genome were constructed. hybridization of 32p-labeled cloned and gel-purified hindiii, ecori, and xbai fragments to southern blots of hindiii-, ecori-, and xbai-cleaved gpcmv dna verified the viral origin of cloned fragments and allowed construction of hindiii, ecori, and xbai restri ... | 1984 | 6092669 |
| structure of the heterogeneous l-s junction region of human cytomegalovirus strain ad169 dna. | the genome of human cytomegalovirus strain ad169 contains a region of heterogeneity located at the junction between the long (l) and short (s) components of the viral dna. twelve cloned l-s junction fragments were studied by using the restriction enzymes haeii and xhoi. the region of heterogeneity was localized within a single haeii restriction fragment. the enzyme xhoi was used to subdivide this region and revealed the presence of three types of heterogeneity within the junction fragments. each ... | 1984 | 6092675 |
| a human nucleolar antigen (pp90) associated with cell growth and its induction by epstein-barr virus and human cytomegalovirus. | immunofluorescent staining of hela cells with rabbit antiserum raised against isolated hela cell nucleoli showed bright nucleolar fluorescence. immunoprecipitation of nuclear extracts obtained from hela cells labelled with 35s-methionine or 32p-orthophosphate followed by gel electrophoresis of the precipitate revealed a major band of 90 kd. this antigen, called pp90, was judged to be responsible for the nucleolar fluorescence. serine residues were predominantly phosphorylated in pp90. similar nu ... | 1984 | 6094364 |
| characterization of the human cytomegalovirus envelope glycoproteins. | virions of human cytomegalovirus were shown to contain two discrete membranes. an outer, loose fitting, membrane was sensitive to osmotic shock and could be partially removed by diluting buffered preparations of virions with water. purified virions were shown, by sds-polyacrylamide gel electrophoresis of virion membranes labelled by carbohydrate-specific procedures, to contain five glycoproteins with molecular weights of 52, 67, 95, 130 and 250, all x 10(3). digestion of virions with endoglycosi ... | 1984 | 6094713 |
| human cytomegalovirus infections in vitro after treatment with arildone. | arildone (win 38020), a broad spectrum antiviral, aryl-beta-diketone (4-[6-(2-chloro-4-methoxy)phenoxyl]hexyl-3,5-heptanedione), blocks the replication of human cytomegalovirus at a stage prior to the synthesis of virus-specific dna. inhibitory action was demonstrated against a number of virus isolates from neonates and immune-compromised patients. intranuclear sites of virus replication, highlighted by dna-staining methods or immunofluorescence, were absent after arildone treatment and correspo ... | 1984 | 6096491 |
| inhibition of human cytomegalovirus replication by 9-(1,3-dihydroxy-2-propoxymethyl)guanine alone and in combination with human interferons. | the inhibitory action of 9-(1,3-dihydroxy-2-propoxymethyl)guanine on the replication of human cytomegalovirus was studied. three laboratory strains (ad-169, towne, and davis) and three early passage (less than 10) clinical isolates were all inhibited in yield inhibition assays. in cultures infected with ad-169, virus yields could be inhibited if the drug was added as late as 3 days after the replication cycle had begun. the effects of the drug were fully reversible during the first 4 days of the ... | 1984 | 6097163 |
| the immune response to human cytomegalovirus-induced early nuclear and early membrane antigens and its possible clinical significance. | before replication of the human cytomegalovirus (hcmv) genome takes place, two major antigenic complexes are induced in the infected cell by an early expression of parental viral genome: one (early antigen or ea) is localised in the nucleus, the other (early membrane antigen or ema) on the plasma-membrane. sequential samples of serum from renal transplant recipients, pregnant women, blood donors and patients with known hcmv disease were examined by means of indirect immunofluorescence tests for ... | 1984 | 6098617 |
| bwb759u, an analogue of acyclovir, inhibits human cytomegalovirus in vitro. | 1984 | 6148640 | |
| murine monoclonal antibody to a single protein neutralizes the infectivity of human cytomegalovirus. | murine monoclonal antibodies to human cytomegalovirus (cmv) strain ad169 were selected that neutralized virus infectivity. one monoclonal antibody-producing hybridoma, 1g6, was used to produce ascites fluid from which immunoglobulin was isolated. this antibody efficiently neutralized cmv ad169, other laboratory strains (towne, davis), and clinical isolates of cmv in early tissue culture passage (less than 10) in the absence of complement. the antibody immunoprecipitated a single 86,000-dalton pr ... | 1984 | 6199788 |
| detection of human cytomegalovirus in clinical specimens by dna-dna hybridization. | a diagnostic assay has been developed to detect human cytomegalovirus (hcmv) dna in clinical specimens with 32p-labeled cloned fragments of hcmv strain ad169. the labeled probe can detect 10 pg of hcmv dna and fails to hybridize to dna from other herpesviruses or human dna. the assay correctly identified 22 (92%) of 24 coded urine specimens culture positive for hcmv and 23 (88%) of 26 urine specimens culture negative for hcmv. in a prospective study of 67 buffy-coat specimens from recipients of ... | 1984 | 6086767 |
| human cytomegalovirus dna sequences with homologies to the cellular genome. | dna from a cosmid-cloned gene library of human cytomegalovirus (hcmv) strain ad169 was found to share sequence homologies with cellular dna of various origins. viral dna fragments subcloned in plasmid vectors enabled localization of the homologous sequences to five regions of the hcmv genome. four of these regions were found in the long unique (ul) segment of the viral genome (ecori fragments r, i and b, and hindiii fragment s); one region with virus--cell homology was located in the terminal in ... | 1984 | 6086821 |
| small fragments of herpesvirus dna with transforming activity contain insertion sequence-like structures. | a 737-base-pair fragment of herpes simplex virus type 2 dna with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the bgl ii n fragment region (map position 0.58-0.625), which were constructed in vitro. the transforming sequences lie within the coding region for a mr 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. contained within the transforming fragment are sequences that can b ... | 1984 | 6087345 |
| dexamethasone enhances human cytomegalovirus replication in human epithelial cell cultures. | an epithelial human hepatoma cell line (plc/prf/5) and a primary epithelial human baby kidney (hbk) cell culture showed restricted growth of human cytomegalovirus (hcmv). treatment of these two epithelial cell cultures with dexamethasone greatly enhanced their ability to support hcmv replication. growth kinetic experiments and infectious center assay revealed that in both the hormone-treated cultures infectious progeny virus appeared earlier by 1 or 2 days and 5- or 10-fold more cells are able t ... | 1984 | 6087554 |
| early and late antigens of human cytomegalovirus: electroimmunodiffusion assay of numbers, relationships, and reactivities with donor sera. | early antigens (ea) of human cytomegalovirus extracted from cytosine arabinoside-blocked cells infected with 0.01-20 infectious units (iu)/cell were assayed with human serum by electroimmunodiffusion (eid). the number of detectable ea types increased from one to eight as the iu/cell was raised from 0.01 to 10. there was no increase in the number of ea with further increases in iu/cell, with prolonged culture, or when detergent was included in the extraction buffer. at least five of the eight ea ... | 1985 | 2983008 |
| inhibition of cellular dna polymerase alpha and human cytomegalovirus-induced dna polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine. | the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine were examined for their inhibitory effect on highly purified cellular dna polymerase alpha and human cytomegalovirus (towne strain)-induced dna polymerase. these two nucleoside triphosphates competitively inhibited the incorporation of dgmp into dna catalyzed by the dna polymerases. the virus-induced dna polymerase had greater binding affinity for the triphosphate of 9-(2-hydroxyethoxymethyl)guanin ... | 1985 | 2983088 |
| antiprostate carcinoma monoclonal antibody (d83.21) cross reacts with a membrane antigen expressed on cytomegalovirus-transformed human fibroblasts. | virological and epidemiological studies have implicated human cytomegalovirus (hcmv) as a possible etiological agent of prostate cancer. because of the suspected associations, this laboratory tested the reactivity of a prostate-associated monoclonal antibody with hcmv-transformed cells. this mouse monoclonal antibody, d83.21, reacts with a membrane antigen on prostate and bladder tumor cells and does not bind to a variety of other malignant or normal cells. the results of this study indicated th ... | 1985 | 2983294 |
| precise localization of genes on large animal virus genomes: use of lambda gt11 and monoclonal antibodies to map the gene for a cytomegalovirus protein family. | we describe an efficient procedure, which uses monoclonal antibodies directed against specific viral proteins, for the precise mapping of genes on large dna virus genomes. we have used the technique to locate the gene encoding a family of antigenically related dna-binding proteins on the 240-kilobase-pair human cytomegalovirus (cmv) genome. a random library of cmv dna fragments was generated using the prokaryotic vector lambda gt11, which expresses open reading frames as beta-galactosidase fusio ... | 1985 | 2983334 |
| on the intracellular transport and the nuclear association of human cytomegalovirus structural proteins. | in cells productively infected with human cytomegalovirus (hcmv) ad169, large amounts of two viral proteins, the 150k major capsid and the 68k major matrix proteins, are continuously produced during the late phase of infection. in the present study, the mechanism for the intracellular transport of the 150k and 68k proteins was investigated. infected cells were labelled for 30 min at 72 h post-infection with [35s]methionine, chased for various periods of time at 37 degrees c, and fractionated int ... | 1985 | 2984313 |
| a very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus. | a strong transcription enhancer was identified in the genomic dna (235 kb) of human cytomegalovirus (hcmv), a ubiquitous and severe pathogen of the herpesvirus group. cotransfection of enhancerless sv40 dna with randomly fragmented hcmv dna yielded two sv40-hcmv recombinant viruses that had incorporated overlapping segments of hcmv dna to substitute for the missing sv40 enhancer. within hcmv, these enhancer sequences are located upstream of the transcription initiation site of the major immediat ... | 1985 | 2985280 |
| the structure of the major immediate early gene of human cytomegalovirus strain ad169. | the nucleotide sequence of the major immediate early (ie) gene of human cytomegalovirus strain ad169 was determined. the structure of the gene was examined by nuclease mapping and by sequence analysis of a cdna clone made from ie mrna. the gene encodes a spliced molecule of 1736 nucleotides, made up of four exon sequences of 121, 88, 185 and 1342 nucleotides. three introns (827, 114 and 170n) were located near the 5' end of the gene. a single open reading frame starting in the second exon extend ... | 1985 | 2986374 |
| catalytic properties of a human cytomegalovirus-induced protein kinase. | human cytomegalovirus, a dna virus whose genome contains a fragment of transforming dna, induces a threonine-serine protein kinase having a molecular mass of 68 kda (p68). p68 was extracted from cells 96-144 h after infection, and immunoprecipitated with a monoclonal antibody (f6b). antibody-enzyme complexes were immobilized on heat/formaldehyde-inactivated staphylococcus aureus. the best substrates for p68 were acidic proteins, phosvitin and casein. glycogen synthase, phosphorylase alpha and hi ... | 1985 | 2986975 |
| cytomegalovirus dna in colorectal carcinoma tissues. | numerous studies had linked human cytomegalovirus (hcmv) infections with neoplasia. among various other malignant tumors, colonic carcinoma tissues were reported to contain dna sequences hybridizing with dna extracted from virus particles. gene technology allowed us to use a cloned viral dna library to measure hcmv in colorectal tumors more specifically. four of 38 tissue specimens did contain dna sequences homologous to cloned viral dna probes; however, in each of those cases, identical hybridi ... | 1985 | 2987605 |
| inhibition of human cytomegalovirus by combined acyclovir and vidarabine. | the inhibition of human cytomegalovirus (hcmv) isolates by acyclovir (acv) and vidarabine (ara-a) was assessed by using an infectious-center plaque-reduction assay. when fixed concentrations of 4.5 micrograms of acv and 250 ng of ara-a per ml were compared singly and in combination, the viral inhibition resulting from the acv-ara-a combination was synergistic for three of four hcmv clinical isolates studied and additive for one hcmv isolate. an additional four hcmv strains obtained at postmortem ... | 1985 | 2988432 |
| monocyte-derived inhibitor of interleukin 1 induced by human cytomegalovirus. | it has previously been shown that human cytomegalovirus (hcmv) can exert immunosuppressive effects, and it has been suggested that these may be mediated by monocytes, although the mechanism is unclear. we showed that infection of human monocytes with the ad169 strain of hcmv abrogates their production of interleukin 1 (il-1) activity. this was associated with the release from infected monocytes of an inhibitor of il-1 activity which was also released after hcmv infection of the u937 macrophage-l ... | 1985 | 2991578 |
| cell-related sequences in the dna genome of human cytomegalovirus strain ad169. | human cytomegalovirus (hcmv) cloned ecori fragments r and b hybridized strongly, under standard high-stringency conditions, to uninfected cellular dna of human, murine, or sea urchin origin. less hybridization was detected with fragments, a, c, e, wl(f), wn(h), i, m, o, p, q, v, c, d, and e. southern blot analysis of the hcmv-related human dna localized the major sites of hybridization of hcmv ecori fragments r, b, and d to defined regions of the 28s rrna gene. | 1985 | 2991601 |
| the use of dna probes in studies of human cytomegalovirus. | hybridization assays provide a sensitive and rapid means for studying the molecular biology of viral replication and for identifying viral nucleic acid in biological specimens. such assays are attractive because the detection of virus does not require intact virions or concomitant viral protein synthesis, both of which may be absent in a latently infected cell or in a virus-associated tumor. for molecular and clinical studies on human cytomegalovirus (hcmv), we have cloned and characterized subg ... | 1985 | 2992841 |
| sequences in human cytomegalovirus which hybridize with the avian retrovirus oncogene v-myc are g + c rich and do not hybridize with the human c-myc gene. | the degree of relatedness between previously identified cross-hybridizing regions within human cytomegalovirus strain ad169 and the avian retrovirus oncogene v-myc were investigated by nucleotide sequence comparison. we found that the homologous regions between the human cytomegalovirus genome and v-myc are limited to short g + c-rich regions in each genome and that the human cytomegalovirus genome shares little or no homology with the human c-myc gene. | 1985 | 2993868 |
| abortive infection with human cytomegalovirus induces an alteration of growth pattern: morphological changes with cytocidal effect in rabbit kidney epithelial cells. brief report. | rabbit kidney epithelial cells (rk13) exhibited a cytopathic effect (cpe) characterized by cell rounding after infection with human cytomegalovirus (hcmv). although hcmv-specific immediate early and early antigens were detected by indirect immunofluorescence techniques, neither late antigens nor infectious progeny virus could be observed in virus-infected rk13 cells. hcmv-infected rk13 cells showed a prolonged doubling time and a decreased saturation density in cell growth compared to uninfected ... | 1985 | 2994600 |
| demonstration of the colinearity of human cytomegalovirus genomes and construction of restriction maps of unknown isolates using cloned subgenomic fragments. | in this study, we have established the colinearity of human cytomegalovirus (hcmv) genomes using stringent conditions of dna-dna filter hybridization of hcmv hindiii fragments and cosmid-cloned ad169 strain hcmv dna fragments. large cosmid-cloned fragments of ad169 dna were used for the preparation of radioactive probes by nick translation. these probes were hybridized to hindiii digests of dna from three fresh isolates of hcmv and to that of the davis strain. using published hindiii restriction ... | 1985 | 2995554 |
| immunological cross-reactivities among three herpesviruses. | sixty adults were tested for humoral and cell-mediated immunity to varicella zoster virus (vzv), type 1 herpes simplex virus (hsv-1) and the human cytomegalovirus (cmv). since herpesviruses share common antigens, we compared results in these individuals to assess whether our tests gave false positives due to cross-reactions. of the 60, igg antibody to vzv, hsv-1, cmv tested by elisa was detected in 51 (85%), 34 (57%) and 20 (33%) respectively. all possible permutations of results were obtained a ... | 1985 | 2997331 |
| detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. | in situ hybridization was used to detect human cytomegalovirus (hcmv) in the peripheral blood mononuclear cells of some naturally infected (seropositive) individuals. a subpopulation of cells hybridized specifically to a portion of the hcmv genome that is heavily transcribed during the immediate-early period of infection. the hybridization signal was markedly reduced by base hydrolysis and ribonuclease, and therefore the probe appears to be detecting viral rna. a fluorescence-activated cell sort ... | 1985 | 2997930 |
| [effect of tunicamycin on human cytomegalovirus replication]. | 1985 | 2998078 | |
| preparation of highly purified human cytomegalovirus envelope antigen. | a human cytomegalovirus (hcmv) envelope preparation was highly purified by immunoaffinity column chromatography using an anti-cellular-igg column. the purified envelope induced high titre antibodies to hcmv in guinea-pigs. analysis of the guinea-pig immune sera by ria and immunofluorescence (if) showed that this envelope preparation, unlike its unpurified counterpart, did not induce antibody to cellular contaminants. dot-blot assay revealed viral proteins in the flow-through fraction and cellula ... | 1985 | 2998113 |
| a murine monoclonal antibody recognising a single glycoprotein within a human cytomegalovirus virion envelope glycoprotein complex. | nonionic detergent solubilised polypeptides from highly purified human cytomegalovirus virions were used as immunogens to produce murine monoclonal antibody secreting hybridomas. one monoclonal antibody was shown, by immunoprecipitation followed by sds-polyacrylamide gel electrophoresis (sds-page), to precipitate three glycoproteins with molecular weights 52, 95, and 130 (all x 10(3)) and one minor component with a molecular weight of 50 x 10(3). when virion envelope components were first separa ... | 1985 | 2999324 |
| autoregulation of the human cytomegalovirus major immediate-early gene. | the gene coding for the human cytomegalovirus major immediate-early 72-kilodalton protein was cloned into simian virus origin of dna replication plasmid psvod. transfection of this plasmid (psvcc2) into cells constitutively expressing the simian virus 40 t-antigen resulted in readily detectable levels of immediate-early region 1-specific rna and protein. partial restriction enzyme digestion of psvcc2 was used to generate specific amino acid deletions within the 72-kilodalton protein. mutant delt ... | 1985 | 2999424 |
| detection of human cytomegalovirus-induced dna-binding proteins on western blots. | dna-binding proteins have been isolated from human embryonic fibroblast cells infected with human cytomegalovirus strain ad169 by native and denatured dna-cellulose affinity chromatography and polymin p precipitation. the dna-binding proteins separated by polyacrylamide gel electrophoresis and electrophoretically transferred to a nitrocellulose sheet were identified by a modified elisa reaction. six dna-binding proteins were found in infected cells, their molecular weights range from 52k to 18k. | 1985 | 3009514 |
| interaction of 9-(1,3-dihydroxy-2-propoxymethyl)guanine with cytosol and mitochondrial deoxyguanosine kinases: possible role in anti-cytomegalovirus activity. | the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) is a potent inhibitor of human cytomegalovirus in vitro and in vivo. in order to investigate the phosphorylation of dhpg to the monophosphate and identify the enzyme responsible, attempts were made to isolate dhpg kinase from calf thymus and from human cytomegalovirus-infected lung cells. from calf thymus, a mitochondrial deoxyguanosine kinase was partially purified which co-migrated with dhpg phosphorylating activity on deae ... | 1985 | 3001505 |
| a long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mrna in murine cytomegalovirus. | using the simian virus 40 "enhancer trap" approach, we have identified a transcription enhancer located just upstream of the major immediate early gene of murine cytomegalovirus. this enhancer has several striking properties. (i) together with the enhancer of human cytomegalovirus, it is the strongest transcription enhancer found to date. (ii) it is an extremely long enhancer, spanning greater than 700 base pairs. (iii) it consists of a rather complex pattern of sequence repeats, the longest of ... | 1985 | 3001696 |
| identification of a 65 000 dalton virion envelope protein of human cytomegalovirus. | human cytomegalovirus (cmv) specific monoclonal antibodies were shown to react with a broadly migrating 65-52 kda virion envelope protein. these antibodies efficiently neutralized cmv in vitro (greater than 98% plaque reduction), suggesting that this envelope protein was an important target of neutralizing antibodies. this virion envelope protein was shown to be antigenically and electrophoretically distinct from a more abundant 68 kda virion protein. the relationship between these virion protei ... | 1985 | 3002068 |
| human natural killer cell lysis of virus-infected cells. relationship to expression of the transferrin receptor. | natural killer (nk) cells lyse tumor and virus-infected cells yet the nature of the target structure they recognize is unknown. a normal host cell glycoprotein, the transferrin receptor (tfr), has been proposed as a target structure on tumor cells. we therefore investigated whether changes in the number or physiological recycling of the tfr, consequent on virus infection, were related to the differential susceptibility of virus-infected cells to nk lysis. there was a direct correlation between t ... | 1986 | 3009202 |
| [studies of the role of human cytomegalovirus infection in the etiology of retinitis and choroiditis. preliminary report]. | 1986 | 3025509 | |
| expression of a human cytomegalovirus late gene is posttranscriptionally regulated by a 3'-end-processing event occurring exclusively late after infection. | a phenomenon of posttranscriptional regulation has been previously identified in cytomegalovirus-infected human fibroblast cells (wathen and stinski, j. virol. 41:462, 1982). a region typifying this phenomenon has been located within the large unique component of the viral genome (map units 0.408 to 0.423). even though this transcriptional unit was highly transcribed at early times after infection, mrnas from this region were only detectable on the polyribosomes after viral dna replication. thus ... | 1986 | 3025644 |
| prospects for the clinical management of human cytomegalovirus infections. | infection of susceptible populations by human cytomegalovirus (hcmv) is a significant public health problem in western societies. vaccination with live attenuated strains of hcmv has demonstrated some degree of clinical benefit but objections based on the possibility of these viruses becoming latent and their potential oncogenicity must be considered. our knowledge of the biology and immunology of hcmv, although advancing rapidly, is still a long way short of being able to predict candidate subu ... | 1986 | 3026105 |
| synthesis and antiherpes virus activity of phosphate and phosphonate derivatives of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine. | a series of phosphate esters of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) were synthesized and evaluated for antiherpes virus activity. the cyclic phosphate esters were made by a new, efficient method utilizing stannic chloride as a solubilizing agent. monophosphate 2 and bisphosphate 4 showed comparable activity to dhpg and probably acted as prodrugs of dhpg. on the other hand, the cyclic phosphate of dhpg 3 was taken up by cells and bypassed the virus-specified thymidine kinase. as ... | 1986 | 3009811 |
| immune responses to isolated human cytomegalovirus envelope proteins. | a group of envelope proteins of human cytomegalovirus, ga protein (l. pereira, m. hoffman, m. tatsuno, and d. dondero, virology 139:73-86, 1984; l. pereira, p. 383-404, in b. roizman, ed., the herpesviruses, vol. 3, 1985), and two protein mixtures (58,000-molecular-weight [58k]-66k and 130k-66k), separated by serial columns prepared with anti-ga immunoglobulin g from sera of immunized guinea pigs, induced neutralizing antibodies and a cellular immune response in the animals. the ga is a disulfid ... | 1986 | 3009877 |
| establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate-early gene products by protein synthesis inhibition. | upon transfection of rat-2-tk- cells with plasmid pes, containing the cloned 7.0-kilobase (kb) ecori-sali fragment (0.063 to 0.089 map units) of the human cytomegalovirus genome, major immediate-early antigen expression was obtained in 1 to 2% of the nuclei of the transfected cells, as determined by immunofluorescence with the e3 monoclonal antibody. cotransfection of pes with the cloned herpes simplex virus type 1 thymidine kinase gene resulted in the establishment of a hypoxanthine-aminopterin ... | 1986 | 3009892 |
| recent clinical isolates of cytomegalovirus suppress human cytomegalovirus-specific human leukocyte antigen-restricted cytotoxic t-lymphocyte activity. | peripheral blood mononuclear cells harvested from healthy adults seropositive for human cytomegalovirus (hcmv) and cultured with laboratory strain ad-169 demonstrated human leukocyte antigen-restricted and hcmv-specific killing on target cells infected with either hcmv laboratory strain ad-169 or recent low-passage hcmv isolates. these results indicated that the determinants recognized by cytotoxic t lymphocytes (ctls) are shared among different strains of hcmv. however, when low-passage isolate ... | 1986 | 3012111 |
| immunoglobulin m to the membrane of uninfected fibroblasts in primary human cytomegalovirus infections. | thirty-two patients with primary human cytomegalovirus (hcmv) infection were examined for presence of antibody to the membrane (me) of uninfected human embryonic lung fibroblasts (helf) using an anti-complement immunofluorescence (acif) test. patients were divided into three groups according to age: less than or equal to 12 months (11 cases), 2-6 years (8 cases), greater than or equal to 13 years (13 cases). convalescent-phase sera from 13 out of 13 of the older and 6 out of 8 of the middle-aged ... | 1986 | 3012285 |
| rabbit kidney cells abortively infected with human cytomegalovirus are arrested in mitotic phase. | in rabbit kidney epithelial cells (rk13) abortively infected with human cytomegalovirus (hcmv), dna synthesis at 1 or 2 days post-infection was enhanced 4 to 5 fold, compared to mock-infected cells. dna analysis by isopycnic centrifugation revealed that the dna newly synthesized in the virus infected rk13 cells was of cellular origin. hcmv infection also caused a marked increase in the mitotic activity of rk13 cells. when semi-confluent rk13 cells were infected more than 20 per cent of cells dem ... | 1986 | 3013126 |
| detection of human cytomegalovirus in urine by dna-dna and rna-dna hybridization. | a diagnostic hybridization assay for detecting human cytomegalovirus (hcmv) dna in urine specimens was developed by using cloned viral dna and in vitro-synthesized rna probes. both probes detected 3 pg of homologous dna and hybridized with dna of hcmv but not with other viral or human cellular dna tested. in 95 urine specimens simultaneously tested by cell culture, the sensitivity of hybridization was at least 83%, and the specificity was at least 92%. this assay will be useful for rapid viral d ... | 1986 | 3014011 |
| characterization of glycoprotein complexes present in human cytomegalovirus envelopes. | three disulphide cross-bridged glycoprotein complexes were immunoprecipitated from purified human cytomegalovirus envelopes using a monoclonal antibody with a specificity for a glycoprotein of mol. wt. 52 x 10(3). these complexes were isolated by electroelution after polyacrylamide gel electrophoresis (page) under non-reducing conditions. compositional analysis of each complex by page under reducing conditions showed that at least two distinct complexes, one containing glycoproteins with mol. wt ... | 1986 | 3014056 |
| fc receptor(s) induced by human cytomegalovirus bind differentially with human immunoglobulin g subclasses. | the igg subclass specificity of fc receptor(s) induced on cells by infection with human cytomegalovirus (hcmv) was studied in a binding assay by using infected cells and purified iodinated igg of various subclasses from hcmv seronegative healthy adult donors. all four human igg subclasses bound to hcmv-infected cells, with the following relative magnitudes: igg1 greater than or equal to igg4 greater than igg2 greater than igg3. the igg subclass specificity of the fc receptor was further analysed ... | 1986 | 3014057 |
| intraocular 9-([2-hydroxy-1-(hydroxymethyl) ethoxy] methyl) guanine levels after intravitreal and subconjunctival administration. | clearance studies in rabbits following intravitreal injection of 400 micrograms of 9-([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl) guanine demonstrated levels of the antiviral at 60 hours above the in vitro id50 for several strains of human cytomegalovirus. intravitreal administration of this new antiviral may have therapeutic application in the treatment of acute retinal necrosis and cytomegalovirus retinitis complicating acquired immune deficiency syndrome (aids). | 1986 | 3014412 |
| nuclear factor 1 interacts with five dna elements in the promoter region of the human cytomegalovirus major immediate early gene. | the human cytomegalovirus (hcmv), a ubiquitous pathogen of the herpesvirus group, has a linear double-stranded dna genome of 235 kb. the expression of its major immediate early gene (ie1) is entirely dependent on host factors, presumably proteins binding to dna elements in the regulatory regions of the gene. we have identified four high-affinity binding sites for nuclear factor 1 (nf1) in the promoter upstream region of ie1 gene between nucleotides -780 and -610, and an additional, even stronger ... | 1986 | 3015602 |
| an illustrated guide to the structure of the human cytomegalovirus genome and a review of transcription data. | 1986 | 3018842 | |
| latent infection of human ovarian teratocarcinoma cells with human cytomegalovirus. brief report. | persistent infection with human cytomegalovirus (hcmv) can be established in cultures of human ovarian teratocarcinoma (pa1) cells, and maintained for more than 200 days. infected cultures maintained at 34 degrees c (pa1cmv34) and 37 degrees c (pa1cmv37) entered crisis and subsequently displayed massive cytopathic effects (cpe), whereas infected cultures maintained at 32 degrees c (pa1cmv32) and 39 degrees c (pa1cmv39) continued to release small amounts of infectious virus until 240 or 151 days ... | 1986 | 3019280 |
| in vitro and in vivo activities of phosphate derivatives of 9-(1,3-dihydroxy-2-propoxymethyl)-guanine against cytomegaloviruses. | the anti-cytomegalovirus activities of four phosphate derivatives of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) were evaluated against human, monkey and murine viruses. the 5'-mono-, 3'5'-bis(mono-), and 3',5'-cyclic monophosphate and 5'-homophosphonate forms of dhpg inhibited virus plaque formation at 1-15 microm. the cyclic phosphate and homophosphonate were more active than the other compounds against murine cytomegalovirus (mcmv) in vitro. in an in vivo mcmv infection model, dhpg homoph ... | 1986 | 3021055 |
| the selection and characterization of human monoclonal antibodies to human cytomegalovirus. | this communication describes the application of epstein-barr virus lymphocyte transformation technology to the production of human monoclonal antibodies specific for human cytomegalovirus. a group of such igg antibodies have been characterized in terms of subclass, light-chain composition, specificity for particular viral proteins and neutralizing capacity. these results have shown that the production of antibodies by transformed lymphocytes is representative of the in vivo human immune response ... | 1986 | 3021800 |
| molecular cloning and analysis of three cdna clones homologous to human cytomegalovirus rnas present during late infection. | three virus-specific clones were isolated from a cdna library synthesized from human cytomegalovirus (ad169)-infected cell rna and cloned into the expression vector lambda gt11. these clones, designated c3, d10, and h10 were each found to express a human cytomegalovirus/beta-galactosidase fusion protein that was reactive with antibody prepared against purified virions. by using the cloned cdna, we were able to identify the transcripts that code for each gene product and study the kinetics of exp ... | 1986 | 3021986 |
| killing of human cytomegalovirus-infected fibroblasts by antiviral antibody and complement. | complement-dependent cytolytic antibodies (cyab) to cytomegalovirus (cmv)-infected fibroblasts were detectable in acute- and convalescent-phase sera from renal allograft recipients (n = 44) and nonimmunocompromised patients (n = 14) with symptomatic cmv infection but not in sera from control donors (n = 75; p less than .001 by wilcoxon rank sum test). renal allograft recipients with secondary cmv infection had the highest levels of cyab activity. activity closely correlated with the serum antibo ... | 1986 | 3001193 |
| stimulating effect of heat shock on the early stage of human cytomegalovirus replication cycle. | the effect of mild heat shock on the replication of human cytomegalovirus (hcmv) was studied in human embryo fibroblasts. treatment of cell cultures at 44 degrees c for 10 min just before infection or at 24 h post infection (p.i.) shortened hcmv eclipse period and enhanced viral replication, while heat shock performed at 48 h p.i. had no effect on the hcmv replication cycle. study of hcmv-induced early and late antigens confirmed that the cellular response to heat shock influences hcmv replicati ... | 1986 | 3033930 |
| kaposi's sarcoma of the conjunctiva and cmv-retinitis in aids. | the number of aids patients is still increasing. in 30-50% of these patients ocular lesions occur, which are of diagnostic and prognostic significance. if the life-span of aids patients lengthens in the future, adequate treatment of the ocular conditions will become increasingly important. the two most important ocular manifestations aids are cmv-retinitis and kaposi's sarcoma of the conjunctiva. dhpg, a new virustatic for human cytomegalovirus, appears promising as treatment for the severe cmv- ... | 1986 | 3034535 |
| analysis of long-term human cytomegalovirus latency in vitro. | human leukocyte interferon (ifn-alpha) and acyclovir (acv) have been used to establish human cytomegalovirus (hcmv) latency in infected human embryo lung fibroblast (hel-f) cells. hcmv latency was maintained for a short interval (less than 9 days) after removal of inhibitors by increasing the incubation temperature. we now report a model system in which hcmv latency has been dramatically extended. hel-f cells pretreated with ifn-alpha (200 iu/ml) and acv (300 microm) were infected with a low moi ... | 1986 | 3034821 |
| a novel selective broad-spectrum anti-dna virus agent. | a new compound has been found, (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine ((s)-hpmpa), that has potent and selective activity against a broad spectrum of dna viruses, including herpes simplex virus (types 1 and 2); varicella zoster virus; thymidine kinase-deficient (tk-) mutants of herpes simplex and varicella zoster virus; human cytomegalovirus; phocid, simian, suid, bovid and equid herpesviruses; african swine fever virus; vaccinia virus; and human adenoviruses. it is also active again ... | 1986 | 3762696 |
| characterization of dna polymerase induced by salmon herpesvirus, oncorhynchus masou virus. | a dna polymerase induced by oncorhynchus masou virus (omv) was isolated from cultured salmon cells infected with omv using sequential ion-exchange column chromatography steps. the properties of the omv polymerase were compared to those of human cytomegalovirus (hcmv) polymerase and polymerase alpha from cherry salmon (masu salmon, o. masou) testes. omv polymerase was clearly distinguished from the other polymerases by its optimum temperature for enzyme activity, 25 degrees c. in an investigation ... | 1986 | 3944590 |
| application of a new medium supplement for propagation and storage of human cytomegalovirus. | a new medium supplement, nu-serum, was evaluated for cultivation of human embryonic lung fibroblasts (hel) and for propagation and storage of human cytomegalovirus (hcmv). nu-serum was comparable to fetal bovine serum (fbs) in promoting rapid growth of hel if they were seeded at a sufficient density. hcmv replicated quite satisfactorily in hel cultured with media supplemented with nu-serum as well as fbs. inactivation of hcmv at 37 c occurred similarly when the medium contained fbs or nu-serum. ... | 1987 | 2830472 |
| viral replication in hela/fibroblast hybrid cells infected with human cytomegalovirus. | three human hybrid cell lines were generated by the fusion of d 98or, a hela cell variant, and tig human diploid fibroblasts. chromosome numbers of the hybrid cells fell between that of d 98or cells and the combined chromosome number of the two cell lines, with three marker chromosomes identical to those of d 98or cells. one hybrid, b-3, produced large amounts of human fibronectin as analyzed by immunohistochemistry. only human cytomegalovirus (hcmv) infected b-3 cells showed positive fluorescen ... | 1987 | 3036044 |
| structural and immunological characterization of the intracellular forms of an abundant 68,000 mr human cytomegalovirus protein. | murine monoclonal antibodies and polyvalent monospecific antisera reactive with an abundant 68,000 mr (p68) human cytomegalovirus (cmv) virion protein were used to characterize this protein within cmv-infected cells. the protein was found to partition within the nucleus and cytoplasm of infected cells. pulse-chase analysis indicated the p68 was degraded into three proteins of 52,000, 51,000 and 50,000 mr which were found only within infected cells. both cellular forms as well as the virion p68 w ... | 1987 | 3037013 |
| epstein-barr virus-transformed lymphoblastoid cell lines as antigen-presenting cells and "augmenting" cells for human cmv-specific th clones. | the ability of epstein-barr virus-transformed lymphoblastoid cell lines (lcl) to present human cytomegalovirus (hcmv) antigen to a panel of hcmv-specific t helper (th) clones was evaluated. among the seven th clones studied, only one clone (sp-cn/t3-16) proliferated well to hcmv presented by both autologous mononuclear cells (mnc) and lcl, and one clone (sp-cn/t3-9) proliferated significantly better to hcmv presented by autologous lcl than by autologous mnc. the majority of the hcmv-specific th ... | 1987 | 3038341 |
| virus-specific serum igg, igm, and iga antibodies in cytomegalovirus mononucleosis patients as determined by immunoblotting technique. | the immune response to individual human cytomegalovirus (cmv) structural polypeptides was studied in paired sera from 15 adult cmv mononucleosis (cmv-mn) patients and healthy controls by immunoblotting technique (ib). igm and igg antibodies to at least 11 structural polypeptides with molecular weights of 28k, 49k, 55k, 57k, 66-70k, 82k, 87k, 110k, 150k, 205k, and 235k were detected in the patients' sera in the serum sample obtained in the acute phase of the disease. iga antibodies to polypeptide ... | 1987 | 3040895 |
| comparison of cytomegalovirus antibodies by means of cf, elisa and immunoblotting techniques. | antibody to human cytomegalovirus in sera of immune blood donors reacted with 12 viral polypeptides of molecular weight 200.000. 150.000, 65.000, 48.000, 34,000. 25.000, 23.000, 21.000 and 18.000. development of cmv infection in renal transplant recipients resulted in increase of antibody reacting with various cmv polypeptides as well as in intensity of this reaction. the highly comparable results for cmv antibody detecting were obtained with immunoblotting and elisa techniques. | 1987 | 2833198 |
| interactions of human cytomegalovirus with leukocytes in vivo: analysis by in situ hybridization. | reactivation of human cytomegalovirus (hcmv) from latency occurs in immunosuppressed individuals and infection is itself immunosuppressive. to better understand the basis for this virally induced impairment of immune function, we have analyzed virus-leukocyte interactions by in situ hybridization. we detected viral dna in 12 viremic patients in the mononuclear cell population, predominantly in cells identified as monocytes by their morphology and by labelling the cells with a monocyte specific m ... | 1987 | 2849021 |
| genomic localization, sequence analysis, and transcription of the putative human cytomegalovirus dna polymerase gene. | the human cytomegalovirus (hcmv)-induced dna polymerase has been well characterized biochemically and functionally, but its genomic location has not yet been assigned. to identify the coding sequence, cross-hybridization with the herpes simplex virus type 1 (hsv-1) polymerase gene was used, as suggested by the close similarity of the herpes group virus-induced dna polymerases to the hcmv dna polymerase. a cosmid and plasmid library of the entire hcmv genome was screened with the bamhi q fragment ... | 1987 | 3023689 |
| factors affecting the detection of cytomegalovirus in urine by sandwich enzyme immunoassays. | some factors influencing the detection of human cytomegalovirus (hcmv) in urine were investigated employing 2 enzyme-linked immunosorbent assays (elisas); one utilised anti-cmv dna polymerase while the other anti-cmv mouse monoclonals as the detecting antibodies. the use of anti-cmv dna polymerase was found to be superior in detecting hcmv in both urine and tissue culture fluids than anti-cmv monoclonals. furthermore, alkaline phosphatase conjugates produced much lower background than did peroxi ... | 1987 | 3027118 |
| cytomegalovirus in urine specimens has host beta 2 microglobulin bound to the viral envelope: a mechanism of evading the host immune response? | we have previously reported that human cytomegalovirus (cmv) from urine specimens cannot be captured onto a solid phase by cmv-specific monoclonal antibodies that can capture cmv grown in vitro. we report here that cmv exists in vivo in body fluids such as urine as beta 2 microglobulin (beta 2 m)-coated particles. we have demonstrated the presence of beta 2m on cmv purified directly from urine by western blotting and have shown that the beta 2m was associated with the viral envelope. urinary cmv ... | 1987 | 3029305 |
| a test for human cytomegalovirus-specific immunoglobulins using a modification of a commercial test kit. | a technique (ig-eia) for the detection of cmv-specific igg, igm and iga in human blood is described. ig-eia utilizes alkaline phosphatase-labeled goat anti-human igg, igm and iga as a detection probe and cmv antigen-coated solid phase from commercial kits. ig-eia is compared to indirect fluorescent assay (ifa) and indirect hemagglutination (iha) for sensitivity and specificity. on sequential samples of blood from a set of patients, ig-eia clearly demonstrated seroconversion in cmv-specific igg a ... | 1987 | 3031114 |
| effect of combinations of difluoromethylornithine (dfmo) and 9[(1,3-dihydroxy-2-propoxy) methyl]guanine (dhpg) on human cytomegalovirus. | both the nucleoside analog 9[(1,3-dihydroxy-2-propoxy)methyl]guanine (ganciclovir; dhpg) and the polyamine synthesis inhibitor difluoromethylornithine (dfmo) have been reported to inhibit replication of cytomegalovirus (cmv) in vitro. in these studies, dhpg inhibited human cmv replication at concentrations of 0.1 microm or greater, while dfmo was active (and then only inconsistently) at 5 mm or greater. (dfmo was added to cells 3 days before virus to maximize polyamine depletion.) however, dhpg ... | 1987 | 3031202 |
| the human cytomegalovirus immediate early gene promoter is a strong promoter in cultured drosophila melanogaster cells. | 1987 | 3031595 | |
| acyclic guanosine analogs as inhibitors of human cytomegalovirus. | the acyclic guanosine analogs r- and s-enantiomers of 9-(3,4-dihydroxybutyl)guanine [(r)- and (s)-dhbg], 9-(4-hydroxybutyl)guanine (hbg), and 9-(2-hydroxyethoxymethyl)guanine (acv) were examined for their effects on human cytomegalovirus (cmv) replication and on cmv dna synthesis in cell culture as well as for their ability as triphosphates to interact with cmv dna polymerase. production of early cmv antigens was not affected. all analogs inhibited cmv dna synthesis and late viral antigen synthe ... | 1987 | 3032096 |
| the two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. | human cytomegalovirus (hcmv) purified from cell culture contains two dominant structural phosphoproteins with apparent molecular weights of 65,000 and 150,000, designated as pp65 and pp150 respectively. the humoral immune response of infected individuals against pp65 is relatively weak and is not always detectable by western blot analyses. this report shows that recent clinical isolates of hcmv do not necessarily have pp65 as a prominent constituent, suggesting that the low immune reaction is du ... | 1987 | 3033138 |
| map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. | human cytomegalovirus particles contain a phosphoprotein of 150,000 (pp150) apparent molecular weight in their matrix; the protein appears particularly reactive in western blot analyses with human antisera. the gene for pp150 was mapped by screening a bacteriophage lambda gt11 cdna expression library with monospecific rabbit antisera. subsequent hybridization of cdna with cosmid and plasmid clones containing the human cytomegalovirus strain ad169 genome mapped the gene to hindiii fragments j and ... | 1987 | 3033266 |