Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| raman evidence that the lyoprotectant poly(ethylene glycol) does not restore nativity to the heme active site of horseradish peroxidase suspended in organic solvents. | resonance raman spectroscopy was used to interrogate the heme active site of horseradish peroxidase (hrp) lyophilized in the presence and absence of the lyoprotectant poly(ethylene glycol) (peg; fw 5000; 0-80% w/w) suspended in acetone, chloroform, or acetonitrile. in aqueous solution, fe(3+)hrp is characterized by a five-coordinate high-spin (5-c hs) heme system. the structure of the heme-active site of hrp in all solvents is perturbed by co-lyophilization of hrp with peg. heme active site stru ... | 2015 | 12099832 |
| enhancer effect of fluorescein on the luminol-h2o2-horseradish peroxidase chemiluminescence: energy transfer process. | the chemiluminescence of the luminol-h2o2-horseradish peroxidase system is increased by fluorescein. fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. the maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. the emission sp ... | 2015 | 9481607 |
| [a sensitive method for quantitative analysis of hydrogen peroxide and oxidase substrates in biological samples]. | the sensitive method for peroxidative assays of hydrogen peroxide (up to 5 nmole in 1 ml) and oxidases' substrates (glucose, ethanol) has been developed by use of a non-cancerogenic chromogen--3,3', 5,5'-tetramethylbenzidine (tmb), horseradish peroxidase and corresponding oxidases. the proposed modification of the known method consists in using decreased concentration of tmb (0.05-0.1 mm) and stopping the reaction by adding of acid to ph 1.4-2.1. this approach allowed to enhance the sensitivity ... | 2015 | 10445279 |
| quantification of peroxide ion passage in dentin, enamel, and cementum after internal bleaching with hydrogen peroxide. | the aim of this study was to evaluate the amount of peroxide passage from the pulp chamber to the external enamel surface during the internal bleaching technique. fifty bovine teeth were sectioned transversally 5 mm below the cemento-enamel junction (cej), and the remaining part of the root was sealed with a 2-mm layer of glass ionomer cement. the external surface of the samples was coated with nail varnish, with the exception of standardized circular areas (6-mm diameter) located on the enamel, ... | 2015 | 22621165 |
| horseradish peroxidase-catalysed in situ-forming hydrogels for tissue-engineering applications. | in situ-forming hydrogels are an attractive class of implantable biomaterials that are used for biomedical applications. these injectable hydrogels are versatile and provide a convenient platform for delivering cells and drugs via minimally invasive surgery. although several crosslinking methods for preparing in situ forming hydrogels have been developed over the past two decades, most hydrogels are not sufficiently versatile for use in a wide variety of tissue-engineering applications. in recen ... | 2015 | 24916126 |
| a sensitive electrochemical biosensor for detection of protein kinase a activity and inhibitors based on phos-tag and enzymatic signal amplification. | a simple, highly sensitive and selective electrochemical assay is developed for the detection of protein kinase a (pka) activity based on the specific recognition utility of phos-tag for kinase-induced phosphopeptides and enzymatic signal amplification. when the substrate peptide was phosphorylated by pka reaction, they could specifically bind with phos-tag-biotin in the presence of zn(2+) through the formation of a specific noncovalent complex with the phosphomonoester dianion in phosphorylated ... | 2015 | 25048450 |
| a simple and sensitive fluorescence based biosensor for the determination of uric acid using h2o2-sensitive quantum dots/dual enzymes. | a novel optical detection system consisting of combination of uricase/hrp-cds quantum dots (qds) for the determination of uric acid in urine sample is described. the qds was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and hrp (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. the hydrogen peroxide produced was able to quench the qds fluorescence, which was proportional to uric ... | 2015 | 25113659 |
| photoinduced electron transfer (pet) based label-free aptasensor for platelet-derived growth factor-bb and its logic gate application. | platelet-derived growth factor-bb (pdgf-bb) is often overexpressed in human malignant tumors as an indicator for tumor angiogenesis. here by the photoinduced electron transfer (pet) between dna-ag fluorescent nanoclusters (ncs) and g-quadruplex/hemin complexes, we present a sensitive label-free fluorescent sensor for pdgf-bb. in the presence of pdgf-bb, the specific conjugation with its aptamer induced the conformational change of the duplex-like dna sequence, releasing the g-quadruplex sequence ... | 2015 | 25150781 |
| a smartphone-based colorimetric reader for bioanalytical applications using the screen-based bottom illumination provided by gadgets. | a smartphone-based colorimetric reader (sbcr) was developed using a samsung galaxy siii mini, a gadget (ipad mini, ipad4 or iphone 5s), integrated with a custom-made dark hood and base holder assembly. the smartphone equipped with a back camera (5 megapixels resolution) was used for colorimetric imaging via the hood and base-holder assembly. a 96- or 24-well microtiter plate (mtp) was positioned on the gadget's screensaver that provides white light-based bottom illumination only in the specific ... | 2015 | 25168283 |
| ectopic expression of arabidopsis rci2a gene contributes to cold tolerance in tomato. | cold is a major stress that limits the quality and productivity of economically important crops such as tomato (solanum lycopersicum l.). generating a cold-stress-tolerant tomato by expressing cold-inducible genes would increase agricultural strategies. rare cold-inducible 2a (rci2a) is expressed in arabidopsis, but its molecular function during cold stress is not fully understood. here we ectopically expressed arabidopsis rci2a in transgenic tomato to evaluate tolerance to cold stress without a ... | 2015 | 25260337 |
| enzymatic removal of paracetamol from aqueous phase: horseradish peroxidase immobilized on nanofibrous membranes. | paracetamol is a widely used as an analgesic and an antipyretic that can easily accumulate in aquatic environments. this study aimed to enhance paracetamol removal efficiency from water by combining the biocatalytic activity of horseradish peroxidase (hrp) with the adsorption of nanofibrous membrane. poly(vinyl alcohol)/poly(acrylic acid)/sio2 electrospinning nanofibrous membrane was prepared with fiber diameters of 200 to 300 nm. the membrane was made insoluble by the thermal cross-linking proc ... | 2015 | 25269844 |
| activation of vegf/flk-1-erk pathway induced blood-brain barrier injury after microwave exposure. | microwaves have been suggested to induce neuronal injury and increase permeability of the blood-brain barrier (bbb), but the mechanism remains unknown. the role of the vascular endothelial growth factor (vegf)/flk-1-raf/mapk kinase (mek)/extracellular-regulated protein kinase (erk) pathway in structural and functional injury of the blood-brain barrier (bbb) following microwave exposure was examined. an in vitro bbb model composed of the ecv304 cell line and primary rat cerebral astrocytes was ex ... | 2015 | 25195697 |
| horseradish peroxidase immobilization on carbon nanodots/cofe layered double hydroxides: direct electrochemistry and hydrogen peroxide sensing. | carbon nanodots and cofe layered double hydroxide composites (c-dots/ldhs) were prepared via simply mixing c-dots and cofe-ldhs. the as-prepared composites were used for the immobilization of horseradish peroxidase (hrp) on the glass carbon (gc) electrode. the electrochemical behavior of the hrp/c-dots/ldhs/gc electrode and its application as a h2o2 biosensor were investigated. the results indicated that hrp immobilized by c-dots/ldhs retained the activity of enzyme and displayed quasi-reversibl ... | 2015 | 25194796 |
| amperometric inhibition biosensors based on horseradish peroxidase and gold sononanoparticles immobilized onto different electrodes for cyanide measurements. | new biosensors based on inhibition for the detection of cyanide and the comparison of the analytical performances of nine enzyme biosensor designs by using three different electrodes: sonogel-carbon, glassy carbon and gold electrodes were discussed. three different horseradish peroxidase immobilization procedures with and without gold sononanoparticles were studied. the amperometric measurements were performed at an applied potential of -0.15v vs. ag/agcl in 50mm sodium acetate buffer solution p ... | 2015 | 25179932 |
| electrical signaling of enzyme-linked immunosorbent assays with an ion-sensitive field-effect transistor. | optical laboratory-based immunoassays, such as enzyme-linked immunosorbent assay (elisa) give a high sensitivity and specificity of various fatal diseases. however, these assays are no longer efficient in on-spot diagnostics of wide-spreading and contagious infections. at this point in time, portable and handhold devices play a pivotal role in infectious diseases with quick diagnostics at or near the site of the disease propagation. in this paper, we demonstrated a novel electrical immunoassay o ... | 2015 | 25240958 |
| antioxidant and antiproliferative activity of stachys glutinosa l. ethanol extract. | ethanol extracts of stachys glutinosa l. (lamiaceae) were investigated for antioxidative properties, as well as antiproliferative action on various cell lines. the antioxidant activities were investigated by abts (2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) assay, dpph (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, β-carotene/linoleic acid assay, scavenging of hydrogen peroxide (horseradish peroxidase test), superoxide anion scavenging, and hypochlorous acid scavenging (taurine t ... | 2015 | 25205114 |
| mir-34c regulates the permeability of blood-tumor barrier via maz-mediated expression changes of zo-1, occludin, and claudin-5. | the purposes of this study were to investigate the potential roles of mir-34c in regulating blood-tumor barrier (btb) functions and its possible molecular mechanisms. the over-expression of mir-34c significantly impaired the integrity and increased the permeability of btb, which were detected in an in vitro btb model by transendothelial electric resistance and horseradish peroxidase flux assays, respectively. meanwhile, real-time quantitative pcr (qrt-pcr), western blot and immunofluorescence as ... | 2015 | 25201524 |
| horseradish peroxidase-encapsulated chitosan nanoparticles for enzyme-prodrug cancer therapy. | among various enzyme-based therapies, enzyme-prodrug therapy (ept) promises minimized side effects in that it activates non-toxic prodrugs locally where the enzymes are placed. the success of such an approach requires high enzyme stability against both structural denaturation and potential immunogenicity. this work examines the efficiency of nanoparticles for enzyme protection in ept applications. specifically, horseradish peroxidase (hrp)-encapsulated chitosan nanoparticles (hrp-csnp) were cons ... | 2015 | 25257586 |
| capillary electrophoresis-chemiluminescence detection for carcino-embryonic antigen based on aptamer/graphene oxide structure. | a new strategy is proposed for determination of carcino-embryonic antigen (cea) based on aptamer/graphene oxide (apt/go) by capillary electrophoresis-chemiluminescence (ce-cl) detection system. cea aptamer conjugated with horseradish peroxidase (hrp) firstly mixes with go, and the cl will be quenched because the stack of hrp-apt on go leads to chemiluminescence resonance energy transfer (cret). when cea exists, the specific combination of hrp-apt and cea can form hrp-apt-cea complex, which disso ... | 2015 | 25299985 |
| electrochemical biotin detection based on magnetic beads and a new magnetic flow cell for screen printed electrode. | the use of the first flow-cell for magnetic assays with an integrated magnet is reported here. the flow injection analysis system (fia) is used for biotin determination. the reaction scheme is based on a one step competitive assay between free biotin and biotin labeled with horseradish peroxidase (b-hrp). the mixture of magnetic beads modified with streptavidin (strep-mb), biotin and b-hrp is left 15 min under stirring and then a washing step is performed. after that, 100 μl of the mixture is in ... | 2015 | 25281162 |
| enzymatic amplification detection of peanut allergen ara h1 using a stem-loop dna biosensor modified with a chitosan-mutiwalled carbon nanotube nanocomposite and spongy gold film. | in this paper, a highly sensitive biosensor was constructed for peanut allergen ara h1 detection. the biosensor was constructed by coating a glassy carbon electrode with a chitosan-mutiwalled carbon nanotube nanocomposite and then adding a spongy gold film via electro-deposition to increase the effective area. the probe switched from an "on" to an "off" state in the presence of target dna, which detached biotin from the electrode surface. this also detached streptavidin-horseradish peroxidase (h ... | 2015 | 25281135 |
| electrochemical magnetoimmunosensing platform for determination of the milk allergen β-lactoglobulin. | a very sensitive magnetoimmunosensor for the determination of β-lactoglobulin (β-lg) is reported in this work. a sandwich configuration involving covalent immobilization of the capture antibody (antiβ-lg) onto activated carboxylic-modified magnetic beads (hooc-mbs) and incubation of the modified mbs with a horseradish peroxidase labeled antibody (hrp-antiβ-lg), is used. the resulting modified mbs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (s ... | 2015 | 25281087 |
| an effective and practical immunohistochemical protocol for bone specimens characterized by hyaluronidase and pepsin predigestion combined with alkaline phosphatase-mediated chromogenic detection. | the aim of this study was to provide an effective procedure for immunohistochemistry (ihc) investigations of bone specimens. samples from rat femoral and human vertebral bone were processed with a detailed and effective ihc protocol summarized here. first, a novel antigen retrieval (ar) method of hyaluronidase combined pepsin predigestion (h+p) was established and the optimal concentration and ph value for ar of bone specimens were determined. second, the newly developed method was compared with ... | 2015 | 25279539 |
| a novel biosensor-based microarray assay for the visualized detection of cyp2c19 *2, *3, *4 and *5 polymorphisms. | a number of studies have indicated that the conversion of clopidogrel to its active metabolite is reduced in patients who carry the cyp2c19 *2, *3, *4 or *5 loss-of-function allele, resulting in decreased response of platelet to clopidogrel treatment and worse cardiovascular outcome. the aim of this study was to develop a novel biosensor-based microarray to visually detect cyp2c19 polymorphisms. | 2015 | 25274959 |
| engineering a horseradish peroxidase c stable to radical attacks by mutating multiple radical coupling sites. | peroxidases have great potential as industrial biocatalysts. in particular, the oxidative polymerization of phenolic compounds catalyzed by peroxidases has been extensively examined because of the advantage of this method over other conventional chemical methods. however, the industrial application of peroxidases is often limited because of their rapid inactivation by phenoxyl radicals during oxidative polymerization. in this work, we report a novel protein engineering approach to improve the ra ... | 2015 | 25335829 |
| direct and mediated electrochemistry of peroxidase and its electrocatalysis on a variety of screen-printed carbon electrodes: amperometric hydrogen peroxide and phenols biosensor. | this study compares the behaviour of direct and mediated electrochemistry of horseradish peroxidase (hrp) immobilised on screen-printed carbon electrodes (spces), screen-printed carbon electrodes modified with carboxyl-functionalised multi-wall carbon nanotubes (mwcnt-spces) and screen-printed carbon electrodes modified with carboxyl-functionalised single-wall carbon nanotubes (swcnt-spces). the techniques of cyclic voltammetry and amperometry in the flow mode were used to characterise the prope ... | 2015 | 25374125 |
| enzyme-linked immunosorbent assay by enhanced chemiluminescence detection for the standardization of estrogenic miroestrol in pueraria candollei graham ex benth. | miroestrol (me) is a potent phytoestrogen from the p. candollei tuberous root. it has been approved for use in clinical trials due to its beneficial effect on disorders associated with estrogen deficiency. to ensure medical efficacy and safety, high performance analytical methods for me analysis are required to standardize products from the p. candollei root. an enhanced chemiluminescence enzyme-linked immunosorbent assay (ecl-elisa) was developed and validated using a polyclonal antibody agains ... | 2015 | 25363375 |
| poly(methyl methacrylate) particles for local drug delivery using shock wave lithotripsy: in vitro proof of concept experiment. | to leverage current local drug delivery systems methodology, there is vast use of polymeric particles serving as drug carriers to assure minimal invasive therapy with little systemic distribution of the released drug. there is an increasing interest in poly(methyl methacrylate) (pmma) serving as carriers in drug delivery. the study aims to develop pmma carriers for localized drug delivery and release system, combining innovative biomaterial technology and shock wave lithotripsy (swl), and to stu ... | 2015 | 25350479 |
| techno-economic analysis of horseradish peroxidase production using a transient expression system in nicotiana benthamiana. | despite the advantages of plant-based transient expression systems relative to microbial or mammalian cell systems, the commercial production of recombinant proteins using plants has not yet been achieved to any significant extent. one of the challenges has been the lack of published data on the costs of manufacture for products other than biopharmaceuticals. in this study, we report on the techno-economic analysis of the production of a standard commercial enzyme, namely, horseradish peroxidase ... | 2015 | 25344434 |
| site-specific conjugation of an antibody-binding protein catalyzed by horseradish peroxidase creates a multivalent protein conjugate with high affinity to igg. | cross-linking proteins offers an approach to enhance the distinct function of proteins due to the multivalent effect. in this study, we demonstrated the preparation of a multivalent antibody-binding protein possessing high affinity to igg by conjugating a number of antibody-binding proteins using the horseradish peroxidase (hrp)-mediated protein conjugation method. by introducing a peptide tag containing a tyrosine (y-tag) to the c-terminus of the model protein, a chimera protein of protein g an ... | 2015 | 25381877 |
| microfluidic chip with thermoresponsive boronate affinity for the capture-release of cis-diol biomolecules. | the polydimethylsiloxane microfluidic chip grafted with poly(n-isopropylacrylamide-3-acrylamidephenylboronic acid) (p(nipaam-co-aapba)) was fabricated by uv-induced grafting polymerization for the capture-release of cis-diol-containing biomolecules by temperature-modulated changes instead of changing the ph value of the mobile phase. based on the optimal time for benzophenone soaking and uv irradiation of grafting polymerization, p(nipaam-co-aapba) was successfully grafted on the polydimethylsil ... | 2015 | 25376697 |
| effects of hypothermia combined with neural stem cell transplantation on recovery of neurological function in rats with spinal cord injury. | the microenvironment of the injured spinal cord is hypothesized to be involved in driving the differentiation and survival of engrafted neural stem cells (nscs). hypothermia is known to improve the microenvironment of the injured spinal cord in a number of ways. to investigate the effect of nsc transplantation in combination with hypothermia on the recovery of rat spinal cord injury, 60 sprague‑dawley female rats were used to establish a spinal cord hemisection model. they were divided randomly ... | 2015 | 25385306 |
| chemically glycosylation improves the stability of an amperometric horseradish peroxidase biosensor. | we constructed a biosensor by electrodeposition of gold nano-particles (aunps) on glassy carbon (gc) and subsequent formation of a 4-mercaptobenzoic acid self-assembled monolayer (sam). the enzyme horseradish peroxidase (hrp) was then covalently immobilized onto the sam. two forms of hrp were employed: non-modified and chemically glycosylated with lactose. circular dichroism (cd) spectra showed that chemical glycosylation did neither change the tertiary structure of hrp nor the heme environment. ... | 2015 | 25479876 |
| synthesis of poly(glutamic acid)-tyramine hydrogel by enzyme-mediated gelation for controlled release of proteins. | an in situ-formed hydrogel was synthesized by enzymatic cross-linking of poly(γ-glutamic acid)-tyramine conjugates (pga-tyr) using horseradish peroxidase (hrp) and hydrogen peroxide (h2o2). the gelation time ranged from 25 s to 5 min was accomplished by tuning the concentration of hrp, h2o2/tyr molar ratio and the degree of substitution (ds) of tyr groups. the storage modulus (g'), cross-link density, and mesh size can be tailored by controlling the h2o2/tyr ratio and ds. the rheological analysi ... | 2015 | 25421870 |
| a glucose-powered antimicrobial system using organic-inorganic assembled network materials. | a new glucose-driven photodynamic antimicrobial system was developed to efficiently kill bacteria and fungi, taking advantage of organic-inorganic network materials encapsulating glucose oxidase and horseradish peroxidase and bioluminescence resonance energy transfer (bret). | 2015 | 25418364 |
| role of camp-dependent protein kinase a activity in low-dose endothelial monocyte-activating polypeptide-ii-induced opening of blood-tumor barrier. | our previous studies demonstrated that low-dose endothelial monocyte-activating polypeptide-ii (emap-ii) can selectively increase the permeability of blood-tumor barrier (btb). in addition, low-dose emap-ii significantly decreases the cyclic adenosine monophosphate (camp) concentration and the protein kinase a (pka) expression level in tumor tissues in the rat c6 glioma model. in this study, an in vitro btb model was used to investigate the potential role of camp/pka signaling cascade in emap-ii ... | 2015 | 25416651 |
| electrochemical immunosensor assay (eia) for sensitive detection of e. coli o157:h7 with signal amplification on a sg-pedot-aunps electrode interface. | a novel electrochemical immunosensor assay (eia) for highly sensitive and specific detection of escherichia coli o157:h7 has been developed. this immunosensor is constructed by the assembly of capture antibody on sg-pedot-aunps composites modified glass carbon electrode. in the presence of target e. coli o157:h7, horseradish peroxidase (hrp)-labeled antibody is captured on the electrode surface to form a sandwich-type system via the specific identification. as a result, e. coli o157:h7 detection ... | 2015 | 25412211 |
| disposable and reliable electrochemical magnetoimmunosensor for fumonisins simplified determination in maize-based foodstuffs. | an electrochemical magnetoimmunosensor involving magnetic beads and disposable carbon screen-printed electrode (cspe) for fumonosins (fb1, fb2 and fb3) has been developed and evaluated through a certified reference material (crm) and beer samples. once the immunochemical reactions took place on the magnetic beads solution, they were confined on the surface of cspe, where electrochemical detection is achieved through the addition of suitable substrate and mediator for enzymatic tracer (horseradis ... | 2015 | 25441412 |
| precise tailoring of tyramine-based hyaluronan hydrogel properties using dmtmm conjugation. | injectable tyramine modified hyaluronic acid (ha-tyr) hydrogels which are bio-orthogonally cross-linked with horseradish peroxidase (hrp) and hydrogen peroxide (h2o2) are excellent candidate biomaterials for drug delivery, regenerative medicine and tissue engineering. ligation of tyramine to ha has been reported using the very well established n-(3-dimethylaminopropyl)-n'-ethylcarbodiimide hydrochloride (edc) and n-hydroxysuccinimide (nhs) chemistry. here we demonstrate the applicability of 4-(4 ... | 2015 | 25439901 |
| automated chemiluminescence immunoassay for a nonionic surfactant using a recycled spinning-pausing controlled washing procedure on a compact disc-type microfluidic platform. | a fully automated and integrated chemiluminescence immunoassay, carried out on a compact disc (cd)-type microfluidic platform, for the detection of alkylphenol polyethoxylates (apneos) is described. the pattern of the cd-type microchip was designed so as to permit the sequential solution delivery of the sample solution, the washing solution and the luminol solution, which are required in the chemiluminescence immunoassay process, along with a designed rotation program for spinning the cd-type mi ... | 2015 | 25435234 |
| pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows' milk. | new rapid method of lateral flow enzyme immunoassay (lfeia) for progesterone detection in whole cows' milk was developed. the test system utilized horseradish peroxidase as a label along with the substrate solution containing 3,3',5,5'-tetramethylbenzidine and dextran sulfate to obtain an insoluble blue colored product of the enzyme reaction on a surface of analytical membrane (test and control lines). several aspects of lfeia were optimized: time of the signal detection, membrane materials and ... | 2015 | 25476365 |
| chemiluminescence lateral flow immunoassay based on pt nanoparticle with peroxidase activity. | a lateral flow immunoassay (lf-immunoassay) with an enhanced sensitivity and thermostability was developed by using pt nanoparticles with a peroxidase activity. the pt nanoparticles were synthesized by citrate reduction method, and the peroxidase activity of pt nanoparticles was optimized by adjusting reaction conditions. the peroxidase activity was estimated by using michaelis-menten kinetics model with tmb as a chromogenic substrate. the kinetics parameters of km and vmax were calculated and c ... | 2015 | 25467480 |
| ultrasensitive electrochemical immunosensor for carbohydrate antigen 19-9 using au/porous graphene nanocomposites as platform and au@pd core/shell bimetallic functionalized graphene nanocomposites as signal enhancers. | a facile and feasible sandwich-type electrochemical immunosensor for ultrasensitive determination of carbohydrate antigen 19-9 (ca19-9) was designed by using au nanoparticles functionalized porous graphene (au-pgo) as sensing platform and au@pd core/shell bimetallic functionalized graphene nanocomposites (au@pd-gra) as signal enhancers. herein, au@pd-gra with a large surface area was prepared for immobilizing plentiful of redox probe-thionine (thi), horseradish peroxidase (hrp) and secondary ant ... | 2015 | 25463643 |
| injectable gelatin derivative hydrogels with sustained vascular endothelial growth factor release for induced angiogenesis. | injectable biomaterials are attractive for soft tissue regeneration because they are handled in a minimally invasive manner and can easily adapt to complex defects. however, inadequate vascularization of the injectable constructs has long been a barrier, leading to necrosis or volume reduction after implantation. in this work, we developed a three-step process to synthesize injectable gelatin-derived hydrogels that are capable of controlling growth factor delivery to induce angiogenesis. in our ... | 2015 | 25462840 |
| identification of heme oxygenase-1 stimulators by a convenient elisa-based bilirubin quantification assay. | the upregulation of heme oxygenase-1 (ho-1) has proven to be a useful tool for fighting inflammation. in order to identify new ho-1 inducers, an efficient screening method was developed which can provide new lead structures for drug research. we designed a simple elisa-based ho-1 enzyme activity assay, which allows for the screening of 12 compounds in parallel in the setting of a 96-well plate. the well-established murine macrophage cell line raw264.7 is used and only about 26µg of protein from ... | 2015 | 25462643 |
| amplified and selective detection of manganese peroxidase genes based on enzyme-scaffolded-gold nanoclusters and mesoporous carbon nitride. | this work has demonstrated an amplified and selective detection platform using enzyme-scaffolded-gold nanoclusters as signal label, coupling with mesoporous carbon nitride (mcn) and gold nanoparticles (gnps) modified glassy carbon electrode (gce). streptavidin-horseradish peroxidase (sa-hrp) has been integrated with gold nanoclusters (gncs) as scaffold using a simple, fast and non-toxic method. the mechanisms of enzymatic amplification, redox cycling and signal amplification by this biosensor we ... | 2015 | 25461185 |
| three-dimensional electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on monolithic and macroporous graphene foam. | a high performance three-dimensional (3d) electrochemical immunosensor was developed for sensitive detection of the tumor biomarker, carcinoembryonic antigen (cea). monolithic and macroporous graphene foam grown by chemical vapor deposition (cvd) served as the scaffold of the free-standing 3d electrode. immuno-recognition interface was fabricated via simple and non-covalent immobilization of antibody using lectin-mediated strategy. briefly, the well-known lectin macromolecule (concanavalin a, co ... | 2015 | 25461170 |
| enzyme incorporated microfluidic device for in-situ glucose detection in water-in-air microdroplets. | droplet generating microfluidic systems can provide miniaturized bioanalytical tools by using the homogenous and high-throughput droplets as nanoreactors. in this study, we demonstrated a sensitive and in-situ glucose monitoring system using water-in-air droplets in an enzyme incorporated microfluidic device. a thin film structure of a glucose oxidase (gox) enzyme immobilized hydrogel was constructed in the middle of the microfluidic channel, and nanoliter scaled water-in-air droplets which cont ... | 2015 | 25461161 |
| chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction. | a novel ligation chain reaction (lcr) methodology for single-nucleotide polymorphism (snp) detection was developed based on luminol-h2o2-horseradish peroxidase (hrp)-mimicking dnazyme-fluorescein chemiluminescence resonance energy transfer (cret) imaging on magnetic particles. for lcr, four unique target-complement probes (x and x(⁎), yg and y(⁎)) for the amplification of k-ras (g12c) were designed by modifying g-quadruplex sequence at 3'-end of yg and fluorescein at 5'-end of y(⁎). after the lc ... | 2015 | 25461149 |
| evaluation of a carbon nanotube-titanate nanotube nanocomposite as an electrochemical biosensor scaffold. | a significant aspect of this work is the development of a multi-wall carbon nanotube (mwcnt)-titanate nanotube (tnt) nanocomposite to serve as a biocompatible scaffold with high conductivity on a biosensor surface. unlike other scaffolds consisting of mwcnts alone or tnts alone, the mwcnt-tnt nanocomposite synergistically provides excellent biocompatibility, good electrical conductivity, low electrochemical interferences and a high signal-to-noise ratio. for comparison, after characterising a sc ... | 2015 | 25460903 |
| immunoassay of paralytic shellfish toxins by moving magnetic particles in a stationary liquid-phase lab-on-a-chip. | in this study, we devised a stationary liquid-phase lab-on-a-chip (slp loc), which was operated by moving solid-phase magnetic particles in the stationary liquid phase. the slp loc consisted of a sample chamber to which a sample and reactants were added, a detection chamber containing enzyme substrate solution, and a narrow channel connecting the two chambers and filled with buffer. as a model system, competitive immunoassays of saxitoxin (stx), a paralytic shellfish toxin, were conducted in the ... | 2015 | 25460894 |
| toxic effects of heavy metal terbium ion on the composition and functions of cell membrane in horseradish roots. | the environmental safety of rare earth elements (rees), especially the toxic effect of rees on plants, has attracted increasing attention. however, the cellular mechanism of this toxic effect remains largely unknown. here, the toxic effects of heavy ree terbium ion [tb(iii)] on the cell membrane of horseradish roots were investigated by using electron microscope autoradiography (emarg) and histochemical methods. the results indicated that tb(iii) was distributed in the extracellular and intracel ... | 2015 | 25450914 |
| advanced lsi-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes. | we have developed a large-scale integrated (lsi) complementary metal-oxide semiconductor (cmos)-based amperometric sensor array system called "bio-lsi" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. in this study, we newly developed a bio-lsi chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of bio-lsi. the light shield created by the top metal layer of the lsi chip significantl ... | 2015 | 25483361 |
| screening of single-stranded dna (ssdna) aptamers against a zearalenone monoclonal antibody and development of a ssdna-based enzyme-linked oligonucleotide assay for determination of zearalenone in corn. | a hypotoxic immunosorbent assay for the detection of zearalenone (zen) was developed, by identifying a single-stranded dna (ssdna) aptamer with high specificity and affinity for a zen monoclonal antibody (mab-zen). ssdna aptamers, which could mimic zen epitopes, were identified using the modified systematic evolution of ligands by an exponential enrichment (selex) technique. the purified mab-zen was coated on microtiter plates as a target recognized by the random oligonucleotide ssdna library. t ... | 2015 | 25485848 |
| self-assembled dipeptide-gold nanoparticle hybrid spheres for highly sensitive amperometric hydrogen peroxide biosensors. | novel self-assembled dipeptide-gold nanoparticle (dp-aunp) hybrid microspheres with a hollow structure have been prepared in aqueous solution by a simple one-step method. diphenylalanine (ff) dipeptide was used as a precursor to form simultaneously peptide spheres and a reducing agent to reduce gold ions to gold nanoparticles in water at 60°c. scanning electron microscopy (sem) and transmission electron microscopy (tem) revealed that formed aunps were localized both inside and on the surface of ... | 2015 | 25483915 |
| topographic distribution of cortical projection cells in the rat subiculum. | the topographic distribution of subicular pyramidal cells, which give rise to projections to the entorhinal cortex, presubiculum, parasubiculum, and the retrosplenial granular cortex, was investigated in the rat using retrograde labeling with wheat germ agglutinin-horseradish peroxidase. using two-dimensional unfolded maps of the entire hippocampal and parahippocampal fields, we found that the cells originating the projections to the above cortical areas were consistently observed throughout the ... | 2015 | 25514386 |
| versatile biosensing platform for dna detection based on a dnazyme and restriction-endonuclease-assisted recycling. | on the basis of a dnazyme and a restriction-endonuclease-assisted target recycling strategy using pd-au alloy nanocrystals to immobilize probe dna on an electrode and catalyze the reduction of h2o2 which amplified signal and promoted the detection sensitivity, a versatile biosensing platform for dna detection was proposed. using p53 and oral cancer genes as models, hemin/g-quadruplex simultaneously acted as a reduced nicotinamide adenine dinucleotide (nadh) oxidase and a horseradish peroxidase ( ... | 2015 | 25493424 |
| nanocrystals of zeolite act as enhanced sensing interface for biosensing of leviteracetum. | in present work, nanocrystals of zeolites were employed as sensing interface for augmented electrochemical sensing of antiepileptic drug, that is, leviteracetum (lev). electrochemical sensing method was developed by depositing nanocrystals of zeolites and horseradish peroxidase (nanocrys zeolites-hrp) onto indium tin oxide (ito)-coated glass surface for lev detection. various stages of biosensor fabrication were characterized by transmission electron microscopy, x-ray diffraction, scanning elect ... | 2015 | 25446962 |
| chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism. | a 2-dimensional imaging system was constructed and applied in measurements of gaseous ethanol emissions from the human palm. this imaging system measures gaseous ethanol concentrations as intensities of chemiluminescence by luminol reaction induced by alcohol oxidase and luminol-hydrogen peroxide-horseradish peroxidase system. conversions of ethanol distributions and concentrations to 2-dimensional chemiluminescence were conducted on an enzyme-immobilized mesh substrate in a dark box, which cont ... | 2015 | 25445620 |
| the three lineages of the diploid hybrid verticillium longisporum differ in virulence and pathogenicity. | verticillium longisporum is an economically important vascular pathogen of brassicaceae crops in different parts of the world. v. longisporum is a diploid hybrid that consists of three different lineages, each of which originated from a separate hybridization event between two different sets of parental species. we used 20 isolates representing the three v. longisporum lineages and the relative v. dahliae, and performed pathogenicity tests on 11 different hosts, including artichoke, cabbage, cau ... | 2015 | 25585057 |
| restriction cascade exponential amplification (rcea) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qpcr. | an alternative to qpcr was developed for nucleic acid assays, involving signal rather than target amplification. the new technology, restriction cascade exponential amplification (rcea), relies on specific cleavage of probe-target hybrids by restriction endonucleases (rease). two mutant reases for amplification (ramp), s17c bamhi and k249c ecori, were conjugated to oligonucleotides, and immobilized on a solid surface. the signal generation was based on: (i) hybridization of a target dna to a ram ... | 2015 | 25583452 |
| reconstruction of atonic bladder innervation after spinal cord injury: a bladder reflex arc with afferent and efferent pathways. | background establishing bladder reflex arcs only with the efferent pathway to induce micturition after spinal cord injury (sci) has been successful. however, the absence of sensory function and micturition desires can lead to serious complications. objectives to reconstruct a bladder reflex arc with both afferent and efferent pathways to achieve atonic bladder innervation after sci. methods a reflex arc was established by microanastomosis of the s2 dorsal root to the peripheral process of the l5 ... | 2015 | 25582052 |
| unraveling the role of hydrogen peroxide in α-synuclein aggregation using an ultrasensitive nanoplasmonic probe. | aggregation of α-synuclein (α-syn) in lewy bodies is largely responsible for the demise and death of dopamine neurons. oxidative stress associated with the aggregation-induced oxidative damage is considered as a possible origin of the toxicity. however, the cellular mechanism of h2o2 in the aggregation of α-syn remains a debate, i.e., whether the aggregation is caused by endogenously secreted or exogenous h2o2 from upstream. here, we report on the development of an ultrasensitive plasmonic assay ... | 2015 | 25590377 |
| horseradish peroxidase-immobilized magnetic mesoporous silica nanoparticles as a potential candidate to eliminate intracellular reactive oxygen species. | horseradish peroxidase-immobilized magnetic mesoporous silica nanoparticles (mmsns-hrp) have been synthesized by a nhs/edc coupling between the amino groups of horseradish peroxidase (hrp) and the carboxyl groups on the mmsns surface. it is found that the immobilized hrp on mmsns still retain high activity and the mmsns-hrp can eliminate the reactive oxygen species (ros) in chinese hamster ovary (cho) cells induced by the addition of h2o2 aqueous solution. further, the fluorescent mmsn-hrp-cd na ... | 2015 | 25587910 |
| oxidation pathways underlying the pro-oxidant effects of apigenin. | apigenin, a natural flavone, is emerging as a promising compound for the treatment of several diseases. one of the hallmarks of apigenin is the generation of intracellular reactive oxygen species (ros), as judged by the oxidation of reduced dichlorofluorescein derivatives seen in many cell types. this study aimed to reveal some mechanisms by which apigenin can be oxidized and how apigenin-derived radicals affect the oxidation of 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein (h(2)dcf), ... | 2015 | 26119779 |
| one-pot synthesis of porphyrin functionalized γ-fe2o3 nanocomposites as peroxidase mimics for h2o2 and glucose detection. | meso-tetrakis(4-carboxyphenyl)-porphyrin-functionalized γ-fe2o3 nanoparticles (h2tcpp-γ-fe2o3) were successfully prepared by one-pot method under hydrothermal conditions and were found to possess intrinsic peroxidase-like activity. the h2tcpp-γ-fe2o3 nanocomposites can catalytically oxidize peroxidase substrate 3,3',5,5'-tetramethylbenzidine (tmb) in the presence of h2o2 to produce a blue color reaction, which can be easily observed by the naked eye. furthermore, kinetic studies indicate that th ... | 2015 | 26117755 |
| [the distribution of map kinase phosphatase-1 in the cerebrospinal fluid-contacting nucleus and its functional contribution to depressive behaviors]. | the purpose of this research is to explore the distribution and expression of map kinase phosphatase-1 (mkp-1) in cerebrospinal fluid (csf)-contacting nucleus in depression, and provide experimental evidence to reveal the biological function and regulatory mechanisms of csf-contacting nucleus in depression. depression model was produced by chronic forced swimming stress (cfss) in sprague-dawley (sd) rats. intracerebroventricular injection of cholera toxin subunit b (ctb) labeled with horseradish ... | 2015 | 25672631 |
| ultrastructural visualization of trans-ciliary rhodopsin cargoes in mammalian rods. | cilia are vital to various cellular and sensory functions. the pathway by which ciliary membrane proteins translocate through the transition zone is not well understood. direct morphological characterization of ciliary cargoes in transit remains lacking. in the vertebrate photoreceptor, rhodopsin is synthesized and transported from the inner segment to the disc membranes of the outer segment (os), which is a modified cilium. to date, the membrane topology of the basal os and the mechanisms by wh ... | 2015 | 25664179 |
| enhancement of colorimetric response of enzymatic reactions by thermally evaporated plasmonic thin films: application to glial fibrillary acidic protein. | we report the enhancement of the colorimetric response of horseradish peroxidase (hrp) and alkaline phosphatase (ap) in bioassays by thermally evaporated silver, gold, copper and nickel thin films. in this regard, a model bioassay based on biotin-avidin interactions was employed. biotin groups and enzymes were introduced to all surfaces using a biotinylated linker molecule and avidin, respectively. the colorimetric response of hrp in the model bioassay carried out on the plasmonic thin films wer ... | 2015 | 25663850 |
| a sensitive chemiluminescent immunoassay for point-of-care testing of repaglinide in natural dietary supplements and serum. | for point-of-care testing of the illegal fortification of repaglinide (rep) in natural dietary supplements, a competitive chemiluminescent immunoassay (clia) was established, using a horseradish peroxidase (hrp)-luminol-h2o2 system for signal amplification. polyclonal antibodies for rep were produced via immunization technique. following optimization of the enzyme reaction time and concentrations of antibody and coating antigen, the method showed a limit of quantification (loq) of 1.0 ng/ml in p ... | 2015 | 25656849 |
| an updated view on horseradish peroxidases: recombinant production and biotechnological applications. | horseradish peroxidase has been the subject of scientific research for centuries. it has been used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a major role in these applications. numerous studies have been conducted on the role of horseradish peroxidase in the plant and its catalytic mechanism. however, little progress has been made in its recombinant production. until now, commercial preparations of horseradish peroxidase are still isolated from plant roots ... | 2015 | 25575885 |
| controlling mesopore size and processability of transparent enzyme-loaded silica films for biosensing applications. | silica-based nanoporous thin films including large mesopores are relevant as enzyme supports for applications in biosensing. the diffusion and immobilization of large biomolecules such as enzymes in such porous films require the presence of large mesopores. creating such morphologies based on a bottom-up synthesis using colloidal templates is a challenge in view of the combination of desired material properties and the robustness of the casting process for the fabrication of thin films. here a s ... | 2015 | 25574584 |
| simultaneous detection of hydrogen peroxide and glucose in human serum with upconversion luminescence. | in this paper, a green upconversion photoluminescence (ucpl) system for the highly sensitive and selective detection of h2o2 and glucose in human sera was developed by utilizing the excellent optical properties of nayf4:yb(3+)/er(3+) upconversion nanoparticles (ucnps). in the presence of h2o2, the colorless 3,3',5,5'-tetramethylbenzidine (tmb) was oxidized into blue oxidized tmb (oxtmb) by assistance of horseradish peroxidase (hrp). and the green ucpl of ucnps was quenched by the oxtmb linearly ... | 2015 | 25569878 |
| development of a fed-batch process for a recombinant pichia pastoris δoch1 strain expressing a plant peroxidase. | pichia pastoris is a prominent host for recombinant protein production, amongst other things due to its capability of glycosylation. however, n-linked glycans on recombinant proteins get hypermannosylated, causing problems in subsequent unit operations and medical applications. hypermannosylation is triggered by an α-1,6-mannosyltransferase called och1. in a recent study, we knocked out och1 in a recombinant p. pastoris cbs7435 mut(s) strain (δoch1) expressing the biopharmaceutically relevant en ... | 2015 | 25567661 |
| gelsolin bound β-amyloid peptides(1-40/1-42): electrochemical evaluation of levels of soluble peptide associated with alzheimer's disease. | a method for the highly sensitive determination of soluable β-amyloid peptides (aβ(1-40/1-42)) that employs a detection bioconjugate of hrp-au-gelsolin as the electrochemical nanoprobe is presented. contrary to previous detection notions that utilized antibodies, which could specifically recognize the n- or c-terminus of peptides, we demonstrate herein that the reported specific binding between gelsolin and aβ might provide an alternative way to evaluate the peptides sensitively and selectively. ... | 2015 | 25562737 |
| caseoperoxidase, mixed β-casein-sds-hemin-imidazole complex: a nano artificial enzyme. | a novel peroxidase-like artificial enzyme, named "caseoperoxidase", was biomimetically designed using a nano artificial amino acid apo-protein hydrophobic pocket. this four-component nano artificial enzyme containing heme-imidazole-β-casein-sds exhibited high activity growth and k(cat) performance toward the native horseradish peroxidase demonstrated by the steady state kinetics using uv-vis spectrophotometry. the hydrophobicity and secondary structure of the caseoperoxidase were studied by ans ... | 2015 | 25562503 |
| enzyme dehydration using microglassification™ preserves the protein's structure and function. | controlled enzyme dehydration using a new processing technique of microglassification™ has been investigated. aqueous solution microdroplets of lysozyme, α-chymotrypsin, catalase, and horseradish peroxidase were dehydrated in n-pentanol, n-octanol, n-decanol, triacetin, or butyl lactate, and changes in their structure and function were analyzed upon rehydration. water solubility and microdroplet dissolution rate in each solvent decreased in the order: butyl lactate > n-pentanol > triacetin > n-o ... | 2015 | 25557848 |
| organically modified silica nanoparticles doped with new acridine-1,2-dioxetane analogues as thermochemiluminescence reagentless labels for ultrasensitive immunoassays. | doped organically modified silica nanoparticles (ormosil nps) with luminescent molecules represent a potent approach to signal amplification in biomolecule labeling. herein, we report the synthesis of new ormosil nps incorporating thermochemiluminescent (tcl) 1,2-dioxetane derivatives to prepare tcl labels for ultrasensitive immunoassay, displaying a detectability comparable to those offered by other conventional luminescence-based systems. amino-functionalized ormosil nps were synthesized for i ... | 2015 | 25542582 |
| a sensitive fluorescent assay for thiamine based on metal-organic frameworks with intrinsic peroxidase-like activity. | metal-organic frameworks (mofs) with tunable structures and properties have recently been emerged as very interesting functional materials. however, the catalytic properties of mofs as enzymatic mimics remain to be further investigated. in this work, we for the first time demonstrated the peroxidase-like activity of copper-based mofs (hkust-1) by employing thiamine (th) as a peroxidase substrate. in the presence of h2o2, hkust-1 can catalyze efficiently the conversion of non-fluorescent th to st ... | 2015 | 25542362 |
| peroxidase-mediated conjugation of corn fiber gum and bovine serum albumin to improve emulsifying properties. | the emulsifying properties of corn fiber gum (cfg), a naturally occurring polysaccharide-protein complex, was improved by kinetically controlled formation of hetero-covalent linkages with bovine serum albumin (bsa), using horseradish peroxidase (hrp). the formation of hetero-crosslinked cfg-bsa conjugates was confirmed using ultraviolet-visible and fourier-transform infrared analyses. the optimum cfg-bsa conjugates were prepared at a cfg:bsa weight ratio of 10:1, and peroxidase:bsa weight ratio ... | 2015 | 25542109 |
| effects of terbium (iii) on signaling molecules in horseradish. | rare earth elements, especially terbium (tb), are high-valence heavy metal elements that accumulate in the environment, and they show toxic effects on plants. signaling molecules regulate many physiological and biochemical processes in plants. how rare earth elements affect signaling molecules remains largely unknown. in the present study, the effects of tb(3+) on some extracellular and intracellular signaling molecules (gibberellic acid, abscisic acid, auxin, h2o2, and ca(2+)) in horseradish le ... | 2015 | 25534291 |
| axogenesis in the antennal nervous system of the grasshopper schistocerca gregaria revisited: the base pioneers. | the antennal nervous system of the grasshopper schistocerca gregaria comprises two parallel pathways projecting to the brain, each pioneered early in embryogenesis by a pair of sibling cells located at the antennal tip. en route, the growth cones of pioneers from one pathway have been shown to contact a guidepost-like cell called the base pioneer. its role in axon guidance remains unclear as do the cellular guidance cues regulating axogenesis in the other pathway supposedly without a base pionee ... | 2015 | 25527188 |
| apohrp-based assay to measure intracellular regulatory heme. | the majority of the heme-binding proteins possess a "heme-pocket" that stably binds to heme. usually known as housekeeping heme-proteins, they participate in a variety of metabolic reactions (e.g., catalase). heme also binds with lower affinity to the "heme-regulatory motifs" (hrm) in specific regulatory proteins. this type of heme binding is known as exchangeable or regulatory heme (rh). heme binding to hrm proteins regulates their function (e.g., bach1). although there are well-established met ... | 2015 | 25525887 |
| magnetic bead and gold nanoparticle probes based immunoassay for β-casein detection in bovine milk samples. | in this work, a double-probe based immunoassay was developed for rapid and sensitive determination of β-casein in bovine milk samples. in the method, magnetic beads (mbs), employed as supports for the immobilization of anti-β-casein polyclonal antibody (pab), were used as the capture probe. colloidal gold nanoparticles (aunps), employed as a bridge for loading anti-β-casein monoclonal antibody (mcab) and horseradish peroxidase (hrp), were used as the amplification probe. the presence of β-casein ... | 2015 | 25522084 |
| a hot-spot-active magnetic graphene oxide substrate for microrna detection based on cascaded chemiluminescence resonance energy transfer. | herein, a cascaded chemiluminescence resonance energy transfer (c-cret) process was demonstrated from horseradish peroxidase (hrp)-mimicking dnazyme-catalyzed luminol-h2o2 to fluorescein and further to graphene oxide (go) when hrp-mimicking dnazyme/fluorescein was in close proximity to the go surface. the proposed c-cret system was successfully implemented to construct three modes of c-cret hot-spot-active substrates (modes i, ii and iii) by covalently immobilizing hrp-mimicking dnazyme/fluoresc ... | 2015 | 25644330 |
| fluorescent, bioactive protein nanoparticles (prodots) for rapid, improved cellular uptake. | a simple and effective method for synthesizing highly fluorescent, protein-based nanoparticles (prodots) and their facile uptake into the cytoplasm of cells is described here. prodots made from bovine serum albumin (nbsa), glucose oxidase (ngo), horseradish peroxidase (nhrp), catalase (ncatalase), and lipase (nlipase) were found to be 15-50 nm wide and have been characterized by gel electrophoresis, transmission electron microscopy (tem), circular dichroism (cd), fluorescence spectroscopy, dynam ... | 2015 | 25642999 |
| stability and structural changes of horseradish peroxidase: microwave versus conventional heating treatment. | effects of conventional heating (ch) and microwave (mw) on the structure and activity of horseradish peroxidase (hrp) in buffer solution were studied. ch incubation between 30 and 45 °c increased activity of hrp, reaching 170% of residual activity (ra) after 4-6 h at 45 °c. ch treatment at 50 and 60 °c caused hrp inactivation: ra was 5.7 and 16.7% after 12 h, respectively. secondary and tertiary hrp structural changes were analyzed by circular dichroism (cd) and intrinsic fluorescence emission, ... | 2015 | 25640719 |
| tight junctions in gerbil von ebner's gland: horseradish peroxidase and freeze-fracture studies. | the permeability of tight junctions to horseradish peroxidase (hrp) and the freeze-fracture appearance of junctional structures were investigated in the von ebner's gland of gerbils. in the tracing study, hrp was either administered topically on the dorsal surface of tongues or injected subepithelially into the connective tissue of vallate papillae for 5-30 min. lingual tissues containing the von ebner's gland were sectioned and examined by light and electron microscopy. in von ebner's glands, t ... | 2015 | 25639455 |
| enzyme and voltage stimuli-responsive controlled release system based on β-cyclodextrin-capped mesoporous silica nanoparticles. | enzyme and voltage stimuli are more biologically compatible in the field of stimuli-responsive systems and release systems based on β-cyclodextrin (cd) and ferrocene (fc) have attracted much attention. herein, mesoporous silica nanoparticles (msns) have been functionalized with the ferrocenyl moiety (fc) which interacts with β-cyclodextrin (β-cd) to form an inclusion complex on the opening of the pores. the size of the inclusion complex results in a barrier to the opening of the pores. based on ... | 2015 | 25639347 |
| horseradish peroxidase and antibody labeled gold nanoparticle probe for amplified immunoassay of ciguatoxin in fish samples based on capillary electrophoresis with electrochemical detection. | this paper describes a new amplified immunoassay with horseradish peroxidase (hrp) and antibody (ab) labeled gold nanoparticles (aunps) probe hyphenated to capillary electrophoresis (ce) with electrochemical (ec) detection for ultrasensitive determination of ciguatoxin ctx1b. aunps were conjugated with hrp and ab, and then incubated with limited amount of ctx1b to produce immunocomplex. the immunoreactive sample was injected into capillary for ce separation and ec detection. enhanced sensitivity ... | 2015 | 25637767 |
| stabilization of prussian blue with polyaniline and carbon nanotubes in neutral media for in vivo determination of glucose in rat brains. | this study demonstrates a new electrochemical microbiosensor for selective in vivo monitoring of glucose in rat brains. the microbiosensor is prepared by using prussian blue (pb)/polyaniline (pani)/multi-walled carbon nanotubes (mwnts) as the electrocatalyst for the reduction and determination of h2o2 generated from the glucose oxidase (gox)-based enzymatic catalytic reaction. pani and mwnts are used to stabilize pb nanoparticles in physiological solutions. as a result, the as-formed three-dimen ... | 2015 | 25631755 |
| chemiluminescence reaction kinetics-resolved multianalyte immunoassay strategy using a bispecific monoclonal antibody as the unique recognition reagent. | the multianalyte immunoassay (mia) has attracted increasing attention due to its high sample throughput, short assay time, low sample consumption, and reduced overall cost. however, up to now, the reported mia methods commonly require multiple antibodies since each antibody can recognize only one antigen. herein, a novel bispecific monoclonal antibody (bsmcab) that could bind methyl parathion and imidacloprid simultaneously was produced by a hybrid hybridomas strategy. a chemiluminescence (cl) r ... | 2015 | 25622025 |
| filling polymersomes with polymers by peroxidase-catalyzed atom transfer radical polymerization. | polymersomes that encapsulate a hydrophilic polymer are prepared by conducting biocatalytic atom transfer radical polymerization (atrp) in these hollow nanostructures. to this end, atrpase horseradish peroxidase (hrp) is encapsulated into vesicles self-assembled from poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (pdms-b-pmoxa) diblock copolymers. the vesicles are turned into nanoreactors by uv-induced permeabilization with a hydroxyalkyl phenone and used to polymerize poly(ethylene gly ... | 2015 | 25619496 |
| design, synthesis, and application of ob2c combinatorial peptide and peptidomimetic libraries. | the "one-bead two-compound" (ob2c) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. an ob2c library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. when live cells are incubated with such ob2c libraries, every bead w ... | 2015 | 25616322 |
| 5-sulfosalicylic acid dihydrate-based pretreatment for the modification of enzyme-linked immunoassay of fluoroquinolones in fishery products. | a simple, rapid sample extraction method for the determination of fqs was developed. fishery samples were extracted with 2% of 5-sulfosalicylic acid dihydrate and the extracts were analyzed directly without any further purification or clean-up procedures. the fqs were determined with standards of 2% of 5-sulfosalicylic acid dihydrate in the concentration range of 0.1-25.6 μg l(-1), and the limit of detection (lod) was 0.1 μg l(-1). the matrix interference originated from fishery samples was elim ... | 2015 | 25611486 |
| glycoconjugates distribution during developing mouse spinal cord motor organizers. | the aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. | 2015 | 25605492 |
| the effect on structural and solvent water molecules of substrate binding to ferric horseradish peroxidase. | ultrafast, multi-dimensional infrared spectroscopy, in the form of 2d-ir and pump-probe measurements, has been employed to investigate the effect of substrate binding on the structural dynamics of the horseradish peroxidase (hrp) enzyme. using nitric oxide bound to the ferric haem of hrp as a sensitive probe of local dynamics, we report measurements of the frequency fluctuations (spectral diffusion) and vibrational lifetime of the no stretching mode with benzohydroxamic acid (bha) located in the ... | 2015 | 25605054 |
| enzymatically cross-linked hyaluronic acid/graphene oxide nanocomposite hydrogel with ph-responsive release. | hyaluronic acid (ha) is made up of repeating disaccharide units (β-1,4-d-glucuronic acid and β-1,3-n-acetyl-d-glucosamine) and is a major constituent of the extracellular matrix. ha and its derivatives which possess excellent biocompatibility and physiochemical properties have been studied in drug delivery and tissue engineering applications. tyramine-based ha hydrogel with good compatibility to cell and tissue has been reported recently. however, inferior mechanical property may limit the biome ... | 2015 | 25598448 |
| a modular dna origami-based enzyme cascade nanoreactor. | in this communication, we present a nanoscale reactor assembled from tuneable and spatially addressable tubular dna origami units. we can controllably combine separate origami units equipped with glucose oxidase (gox) and horseradish peroxidase (hrp), and demonstrate efficient gox/hrp enzyme cascade reaction inside the tube. the reactor could be utilized as a nanoscale diagnostic tool, and modularity of the proposed system would further enable more complex reactions. | 2015 | 25594847 |
| long-term chemiluminescence signal is produced in the course of luminol oxidation catalyzed by enhancer-independent peroxidase purified from jatropha curcas leaves. | isoenzyme c of horseradish peroxidase (hrp-c) is widely used in enzyme immunoassay combined with chemiluminescence (cl) detection. for this application, hrp-c activity measurement is usually based on luminol oxidation in the presence of hydrogen peroxide (h2o2). however, this catalysis reaction was enhancer dependent. in this study, we demonstrated that jatropha curcas peroxidase (jcgp1) showed high efficiency in catalyzing luminol oxidation in the presence of h2o2. compared with hrp-c, the jcgp ... | 2015 | 25511847 |