Publications

probiotic fermented milk containing dietary fiber has additive effects in ibs with constipation compared to plain probiotic fermented milk.	although controversial, probiotics and dietary fiber are commonly used for patients with irritable bowel syndrome (ibs). we evaluated the effects of multistrain probiotics on the symptoms of ibs to determine whether the addition of dietary fi ber had an additive effect on constipation-predominant ibs.	2011	21461068
position statement. part two: maintaining immune health.	the physical training undertaken by athletes is one of a set of lifestyle or behavioural factors that can influence immune function, health and ultimately exercise performance. others factors including potential exposure to pathogens, health status, lifestyle behaviours, sleep and recovery, nutrition and psychosocial issues, need to be considered alongside the physical demands of an athlete's training programme. the general consensus on managing training to maintain immune health is to start wit ...	2011	21446353
autoinducer-2-like activity in lactic acid bacteria isolated from minced beef packaged under modified atmospheres.	fifteen fingerprints (assigned to leuconostoc spp., leuconostoc mesenteroides, weissella viridescens, leuconostoc citreum, and lactobacillus sakei) of 89 lactic acid bacteria (lab) isolated from minced beef stored under modified atmospheres at various temperatures were screened for their ability to exhibit autoinducer-2 (ai-2)-like activity under certain growth conditions. cellfree meat extracts (cfme) were collected at the same time as the lab isolates and tested for the presence of ai-2-like m ...	2011	21477479
effect of chlorhexidine pretreatment on the stability of salivary lactobacilli probiotic in six- to twelve-year-old children: a randomized controlled trial.	this study aimed to investigate the efficacy of chlorhexidine mouthwash disinfection on the stability of lactobacilli probiotic. this study involved 105 children in 3 groups. subjects in group a received chlorhexidine for 2 weeks, subjects of group b received probiotic yogurt containing lactobacillus rhamnosus gg (lgg) at a concentration of 2 × 10(8) colony-forming units per gram for 3 weeks, and subjects in group c received chlorhexidine in the same manner as group a and 24 h after cessation, t ...	2011	21454978
antihypertensive effects of lactobacillus-fermented milk orally administered to spontaneously hypertensive rats.	products fermented with lactic acid bacteria may show antihypertensive effects via substances such as angiotensin i-converting enzyme inhibitor (acei) and ?-aminobutyric acid (gaba). it was previously found that milk fermented with lactobacillus paracasei subsp. paracasei ntu 101 (101fm) or lactobacillus plantarum ntu 102 (102fm) has acei and gaba activities. this study aimed to investigate the antihypertensive effects of 101fm and 102fm orally administered to spontaneously hypertensive rats (sh ...	2011	21446645
role of the probiotic strain lactobacillus paracasei lmgp22043 carried by artichokes in influencing faecal bacteria and biochemical parameters in human subjects.	to evaluate the positive influence of the probiotic strain lactobacillus paracasei lmgp22043 carried by artichokes into the human gut with special reference to faecal bacterial balance, short-chain fatty acid concentrations and enzyme activities in a randomized, double-blind human trial in comparison with probiotic-free artichokes (control).	2011	21447019
complete genome sequencing of lactobacillus acidophilus 30sc, isolated from swine intestine.	lactobacillus acidophilus 30sc has been isolated from swine intestines and considered a probiotic strain for dairy products because of its ability to assimilate cholesterol and produce bacteriocins. here, we report the complete genome sequence of lactobacillus acidophilus 30sc (2,078,001 bp) exhibiting strong acid resistance and enhanced bile tolerance.	2011	21478365
comparative proteomic analysis of lactobacillus plantarum for the identification of key proteins in bile tolerance.	lactic acid bacteria are commonly marketed as probiotics based on their putative or proven health-promoting effects. these effects are known to be strain specific but the underlying molecular mechanisms remain poorly understood. therefore, unravelling the determinants behind probiotic features is of particular interest since it would help select strains that stand the best chance of success in clinical trials. bile tolerance is one of the most crucial properties as it determines the ability of b ...	2011	21447177
the effect of hyperbaric oxygenation on postradiation xerostomia and saliva in patients with head and neck tumours.	the study was designed to evaluate the influence of hyperbaric oxygenation (hbo) therapy on salivary gland function and the growth of salivary streptococcus mutans, lactobacillus and candida albicans in patients with head and neck tumours who had undergone radiation therapy. sixteen patients were included, with radiation doses from 58 to 70 gy. the first examination was performed at baseline before the first hbo therapy (33.7 ± 9 months after radiation therapy), and the second after 20 daily hbo ...	2011	21447949
[effect of lactobacilli on th1/th2 cells balance in primary lymphocytes.]	aim: to analyse the effect of 7 lactobacillus species on proliferation and cytokine production of primary lymphocytes, and explored the effect on the th1/th2 cells balance. methods: the mouse spleen cells were stimulated with active/heat-killed lactobacillus strains at different species and concentrations. after be cultured with bacteria 60 hours, the proliferation of splenocytes were tested by mtt colorimetry, and il-12, ifn-γ, il-4, il-10 and tgf-β were detected by elisa. results: (1) both ali ...	2011	21481313
evaluation of the functional potential of weissella and lactobacillus isolates obtained from nigerian traditional fermented foods and cow's intestine.	the characterisation of 24 lactic acid bacteria (lab) isolates from nigerian traditional fermented dairy foods, including some cow's intestine isolates, was conducted in order to select isolates for potential use as probiotics. lab isolates were identified by partial sequencing the 16s rrna gene as belonging to the species lactobacillus paracasei, lactobacillus brevis and mainly weissella confusa. at the end of a characterisation process, 2 l. paracasei and 2 w. confusa isolates were selected, a ...	2011	21482440
probiotics affect the clinical inflammatory parameters of experimental gingivitis in humans.	to determine the effects of a probiotic milk drink consumed over a period of 28 days, regarding the expression of clinical inflammatory parameters of the oral gingiva during various phases of plaque-induced gingivitis.	2011	21448219
development and evaluation of a safe and effective sugar-free herbal lollipop that kills cavity-causing bacteria.	dental caries (tooth decay) is caused by a specific group of cariogenic bacteria, like streptococcus mutans, which convert dietary sugars into acids that dissolve the mineral in tooth structure. killing cariogenic bacteria is an effective way to control or prevent tooth decay. in a previous study, we discovered a novel compound (glycyrrhizol a), from the extraction of licorice roots, with strong antimicrobial activity against cariogenic bacteria. in the current study, we developed a method to pr ...	2011	21449211
aberrant response to commensal bacteroides thetaiotaomicron in crohn's disease: an ex vivo human organ culture study.	human ex vivo evidence indicating that an inappropriate immune response(s) to nonpathogenic bacteria contributes to disease pathogenesis in pediatric crohn's disease (cd) is limited. the aim of the present study was to compare and contrast the early innate immune response of pediatric "healthy" versus cd mucosa to pathogenic, probiotic, and commensal bacteria.	2011	21484962
effects on weight gain and gut microbiota in rats given bacterial supplements and a high-energy-dense diet from fetal life through to 6 months of age.	the aim of the present study was to assess the long-term effects of a high-energy-dense diet, supplemented with lactobacillus plantarum (lp) or escherichia coli (ec), on weight gain, fattening and the gut microbiota in rats. since the mother's dietary habits can influence offspring physiology, dietary regimens started with the dams at pregnancy and throughout lactation and continued with the offspring for 6 months. the weight gain of group lp was lower than that of groups c (control) and ec (p = ...	2011	21450114
fermentation of calcium-fortified soya milk does not appear to enhance acute calcium absorption in osteopenic post-menopausal women.	ageing women may choose to drink soya milk to reduce menopausal symptoms. as fermentation enriches soya milk with isoflavone aglycones, its beneficial qualities may improve. to reduce osteoporotic risk, however, soya milk must be ca enriched, and it is not known how fermentation affects ca bioavailability. a randomised crossover pilot study was undertaken to compare the ca absorption of fortified soya milk with that of fermented and fortified soya milk in twelve australian osteopenic post-menopa ...	2011	20854699
monoassociation with probiotic lactobacillus delbrueckii ufv-h2b20 stimulates the immune system and protects germfree mice against listeria monocytogenes infection.	in the present study, we investigated the protective effects of lactobacillus delbrueckii ufv-h2b20 on the resistance to listeria monocytogenes infection in gnotobiotic mice. germfree mice or monoassociated mice were infected with l. monocytogenes, and the microbiological and immunological responses were evaluated after 1, 3, and 5 days of infection. monoassociation with l. delbrueckii was capable of protecting mice against death caused by l. monocytogenes and induced a faster clearance of the b ...	2011	20838807
probiotic lactobacilli interfere with streptococcus mutans biofilm formation in vitro.	in clinical studies, probiotic bacteria have decreased the counts of salivary mutans streptococci (ms). we compared the effects of probiotic lactobacillus strains on the biofilm formation of streptococcus mutans. the bacterial strains used included four s. mutans strains (reference strains nctc 10449 and ingbritt and clinical isolates 2366 and 195) and probiotic strains lactobacillus rhamnosus gg, l. plantarum 299v, and l. reuteri strains pta 5289 and sd2112. the ability of ms to adhere and grow ...	2011	20835828
distinct microbiome in pouchitis compared to healthy pouches in ulcerative colitis and familial adenomatous polyposis.	pouchitis occurs in up to 50% of patients with ulcerative colitis (uc) undergoing ileal pouch anal anastomosis (ipaa). pouchitis rarely occurs in patients with familial adenomatous polyposis (fap) who undergo ipaa. our aim was to compare mucosal and luminal flora in patients with uc-associated pouchitis (ucp), healthy uc pouches (huc), and healthy fap pouches (fap).	2011	20845425
dying in yoghurt: the number of living bacteria in probiotic yoghurt decreases under exposure to room temperature.	while probiotic bacteria are successfully used in the treatment of ulcerative colitis, the effect of commercially available probiotic products is still controversial. here, we study whether the number of living probiotic bacteria in yoghurts is altered by an interruption of the cold chain.	2011	20838050
contemporary perspectives on vaginal ph and lactobacilli.	ever since the ph of the vagina was found to be much more acidic than blood and interstitial fluids, a belief going back more than a century has persisted that the vagina is protected from pathogenic organisms by the high level of hydronium ions present. a corollary of this belief is that the ph of the vagina and antipathogen activity is due to colonizing lactobacilli. unfortunately, this dogma lacks empirical research support. the vaginal ph is determined by the interplay between vaginal physio ...	2011	20832044
galactoglucomannan oligosaccharide supplementation affects nutrient digestibility, fermentation end-product production, and large bowel microbiota of the dog.	a galactoglucomannan oligosaccharide (ggmo) obtained from fiberboard production was evaluated as a dietary supplement for dogs. the ggmo substrate contained increased concentrations of oligosaccharides containing mannose, xylose, and glucose, with the mannose component accounting for 35% of dm. adult dogs assigned to a 6 × 6 latin square design were fed 6 diets, each containing a different concentration of supplemental ggmo (0, 0.5, 1, 2, 4, and 8%) that replaced dietary cellulose. total tract d ...	2011	20852078
differences between the normal vaginal bacterial community of baboons and that of humans.	humans and baboons (papio spp.) share considerable anatomical and physiological similarities in their reproductive tracts. given the similarities, it is reasonable to expect that the normal vaginal microbial composition (microbiota) of baboons would be similar to that of humans. we have used a 16s rrna phylogenetic approach to assess the composition of the baboon vaginal microbiota in a set of nine animals from a captive facility and six from the wild. results show that although gram-positive ba ...	2011	20853395
adsorption of temperate phages of lactobacillus delbrueckii strains and phage resistance linked to their cell diversity.	aims: the aim of this work was to study the adsorption step of two new temperate bacteriophages (cb1/204 and cb1/342) of lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. methods and results: the effect of divalent cations, ph, temperature and cell viability on adsorption step was analysed. the ca(2+) presence was necessary for the phage cb1/342 but not for the phage cb1/204. both phages showed to be stable at ph values between 3 an ...	2011	21219557
efficacy of an anaerobic swab transport system to maintain aerobic and anaerobic microorganism viability after storage at -80 degrees c.	an amies agar gel swab transport system was evaluated for its ability to maintain bacterial viability and relative quantity after freezing at -80°c. nine american type culture collection (atcc) bacterial strains were used: 3 anaerobic strains (propionibacterium acnes, peptostreptococcus anaerobius, and clostridium sporogenes) and 6 facultative or strict aerobic bacterial strains (stenotrophomonas maltophilia, escherichia coli ([atcc 25922 and atcc 11775], salmonella enterica subsp. enterica sero ...	2011	21217035
sensorically and antimicrobially active metabolite production of lactobacillus strains on jerusalem artichoke juice.	in the tubers of jerusalem artichoke (helianthus tuberosus l.) the main carbohydrate is the well-known prebiotic inulin, which is a good growth substrate for gut microorganisms. jerusalem artichoke tuber is traditionally consumed boiled or pickled rather than in fermented form. lactic acid bacteria are traditionally used in the production of fermented foods; nevertheless their behavior and metabolite production are considerably influenced by the substrate. the purpose of this study was to invest ...	2011	21213229
characterization of lactic acid bacteria from musts and wines of three consecutive vintages of ribeira sacra.	this study was designed to isolate and characterize the lactic acid microbiota of the musts and wines of a young denomination of origin area, ribeira sacra in north-west spain.	2011	21204877
fish analysis of lactobacillus biofilms in the gastrointestinal tract of different hosts.	to investigate the spatial organization of endogenous and exogenously applied lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts.	2011	21204880
bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri.	to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage.	2011	21204884
administration of lactobacillus gg to hospitalised children reduces the risk of gastrointestinal and respiratory tract infections.		2011	21163787
in vitro fermentation of rice bran combined with lactobacillus acidophilus 14 150b or bifidobacterium longum 05 by the canine faecal microbiota.	the fermentability of rice bran (rb), alone or in combination with one of two probiotics, by canine faecal microbiota was evaluated in stirred, ph-controlled, anaerobic batch cultures. rb enhanced the levels of bacteria detected by probes bif164 (bifidobacteria) and lab158 (lactic acid bacteria); however, addition of the probiotics did not have a significant effect on the predominant microbial counts compared with rb alone. rb sustained levels of bifidobacterium longum 05 throughout the fermenta ...	2011	21204868
comparison of alginate and pectin based beads for production of poultry probiotic cells.	a comparative study on the stability and potential of alginate and pectin based beads for production of poultry probiotic cells using mrs medium in repeated batch fermentation was conducted. the bead cores, made of three types of materials, i.e., ca-alginate, ca-pectinate and ca-alginate/pectinate, were compared. the effect of single and double layer coatings using chitosan and core material, respectively, on the bead stability and cell production were also studied. the pectin based beads were f ...	2011	21216192
distinctive anti-allergy properties of two probiotic bacterial strains in a mouse model of allergic poly-sensitization.	we compared the immunomodulatory properties of bifidobacterium longum ncc 3001 and lactobacillus paracasei ncc 2461 in a mouse model of poly-sensitization to birch and grass pollen allergens. mucosal application of both strains at the time of sensitization and challenge led to significant suppression of airway inflammation and down-regulated allergen-specific immune responses. in contrast, in the mice treated with probiotics prior to sensitization and challenge, only b. longum displayed protecti ...	2011	21216308
fluorescence and atomic force microscopy imaging of wall teichoic acids in lactobacillus plantarum.	although teichoic acids are major constituents of bacterial cell walls, little is known about the relationships between their spatial localization and their functional roles. here, we used single-molecule atomic force microscopy (afm) combined with fluorescence microscopy to image the distribution of wall teichoic acids (wtas) in lactobacillus plantarum, in relation with their physiological roles. phenotype analysis of the wild-type strain and of mutant strains deficient for the synthesis of wta ...	2011	21218855
green kiwifruit modulates the colonic microbiota in growing pigs.	to investigate whether green kiwifruit modulates the composition of colonic microbiota in growing pigs.	2011	21255058
evaluation of helicobacter pylori eradication by triple therapy plus lactobacillus acidophilus compared to triple therapy alone.	the purpose of this study was to evaluate the influence of adding lactobacillus acidophilus to a triple regimen for helicobacter pylori eradication in untreated patients with peptic ulcers or ulcer-scars. this was a pre-randomized, single-blind, interventional, treatment-efficacy study with active controls and parallel-assignment, set in coimbra, portugal, on 62 consecutive h. pylori-positive untreated adults with peptic ulcers or ulcer-scars, diagnosed by gastroduodenoscopy, with pre-treatment ...	2011	21207091
characterization and transfer of antibiotic resistance in lactic acid bacteria from fermented food products.	the study provides phenotypic and molecular analyses of the antibiotic resistance in lactic acid bacteria (lab) from fermented foods in xi'an, china. lab strains (n = 84) belonging to 16 species of lactobacillus (n = 73), and streptococcus thermophilus (n = 11) were isolated and identified by sequencing their 16s rrna gene. all strains were susceptible to ampicillin, bacitracin, and cefsulodin, and intrinsically resistant to nalidixic acid, kanamycin, and vancomycin (except l. bulgaricus, l. aci ...	2011	21212956
heat-killed body of lactobacillus brevis sbc8803 ameliorates intestinal injury in a murine model of colitis by enhancing the intestinal barrier function.	background:: probiotics have been clinically administered to improve intestinal damage in some intestinal inflammations. however, probiotic treatments are not always effective for these intestinal disorders because live bacteria must colonize and maintain their activity under unfavorable conditions in the intestinal lumen when displaying their functions. this study investigated the physiological functions of a heat-killed body of a novel probiotic, lactobacillus brevis sbc8803, on the protection ...	2011	21213286
predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium animalis subsp. lactis bi-07.	the effect of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium lactis bi-07 on the composition of the lactobacillus group, bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. the study included 50 children randomized to intake of one of the probiotic strain or placebo. microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative pcr and, in a subset of subjects, by pyrose ...	2011	21204871
global transcriptional analysis of spontaneous sakacin p-resistant mutant strains of listeria monocytogenes during growth on different sugars.	subclass iia bacteriocins have strong antilisterial activity and can control the growth of listeria monocytogenes in food. however, l. monocytogenes may develop resistance towards such bacteriocins. in this follow-up study, the transcriptomes of a high level (l502-1) and a low level (l502-6) spontaneous sakacin p-resistant mutant strain of l. monocytogenes were compared to the wild-type (l502). the growth of the resistant strains was reduced on mannose but not affected on cellobiose and the tran ...	2011	21253597
intraspecies discrimination of lactobacillus paraplantarum by pcr.	lactobacillus paraplantarum is a species phenotypically close to lactobacillus plantarum. several pcr methods were evaluated to discriminate l. paraplantarum strains and among them, a pcr using an enterobacterial repetitive intergenic consensus (eric) sequence differentiated l. paraplantarum from other lactobacillus species. in addition, a combination of eric and random amplified polymorphic dna (rapd) analysis distinguished among seven strains of l. paraplantarum tested. eric-pcr profiles showe ...	2011	21204929
strain-dependent proliferation in response to human gastric mucin and adhesion properties of helicobacter pylori are not affected by co-isolated lactobacillus sp.	helicobacter pylori colonize the mucus layer that covers the gastric epithelium and can cause gastritis, ulcers, and gastric cancer. recently, lactobacillus sp. have also been found to reside in this niche permanently. this study compares adhesive properties and proliferation of co-isolated lactobacilli and h. pylori in the presence of mucins and investigates possibilities for lactobacilli-mediated inhibition of h. pylori.	2011	21241407
phylogenomic reconstruction of lactic acid bacteria: an update.	lactic acid bacteria (lab) are important in the food industry for the production of fermented food products and in human health as commensals in the gut. however, the phylogenetic relationships among lab species remain under intensive debate owing to disagreements among different data sets.	2011	21194491
expression of integrins and toll-like receptors in cervical cancer: effect of infectious agents.	we hypothesized that development of cervical cancer is associated with alterations in the expression of innate immune receptors, i.e. integrins and tlrs, and that these alterations can be induced by infectious agents. we have studied the expression of these proteins in cervical biopsy tissues and cervical cancer-derived cell lines hela, caski, siha, c-33 a, and me180. immunohistochemistry analysis demonstrated an increase in integrin αv, β3, β4, and β6 expression in the epithelium during the dev ...	2011	21239458
structure and functions of exopolysaccharide produced by gut commensal lactobacillus reuteri 100-23.	lactobacillus reuteri strain 100-23 together with a lactobacillus-free mouse model, provides a system with which the molecular traits underpinning bacterial commensalism in vertebrates can be studied. a polysaccharide was extracted from sucrose-containing liquid cultures of strain 100-23. chemical analysis showed that this exopolysaccharide was a levan (β-2, 6-linked fructan). mutation of the fructosyl transferase (ftf) gene resulted in loss of exopolysaccharide production. the ftf mutant was ab ...	2011	21248858
potential beneficial properties of bacteriocin-producing lactic acid bacteria isolated from smoked salmon.	aims: to evaluate the probiotic properties of strains isolated from smoked salmon and previously identified as bacteriocin producers. methods and results: strains lactobacillus curvatus et06, et30 and et31, lactobacillus fermentum et35, lactobacillus delbrueckii et32, pediococcus acidilactici et34 and enterococcus faecium et05, et12 and et88 survived conditions simulating the gastrointestinal tract (git) and produced bacteriocins active against several strains of listeria monocytogenes, but pr ...	2011	21251174
the vaginal microbiome: new information about genital tract flora using molecular based techniques.	vaginal microbiome studies provide information that may change the way we define vaginal flora. normal flora appears dominated by one or two species of lactobacillus. significant numbers of healthy women lack appreciable numbers of vaginal lactobacilli. bacterial vaginosis (bv) is not a single entity, but instead consists of different bacterial communities or profiles of greater microbial diversity than is evident from cultivation-dependent studies. bv should be considered a syndrome of variable ...	2011	21251190
fish analysis of lactobacillus biofilms in the gastrointestinal tract of different hosts.	aims: to investigate the spatial organization of endogenous and exogenously applied lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. methods and results: samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a lactobacillus probiotic were analysed by fluorescence in situ hybridization (fish) with rrna-targeted probes. high levels of endogenous lactobacilli were observed on the nonsecretory, ...	2011	21223342
effects of multistrain probiotic-containing yogurt on second-line triple therapy for helicobacter pylori infection.	the adjuvant effects of probiotic-containing yogurt on second-line triple therapy for helicobacter pylori (h. pylori) infection have not been evaluated.	2011	21175792
use of caenorhabditis elegans for preselecting lactobacillus isolates to control salmonella typhimurium.	host-specific probiotics have been used to control enteric pathogens, including foodborne pathogens, in food animal production. however, evaluation of the efficacy of these probiotics requires costly in vivo assays in the target animal. the nematode caenorhabditis elegans has been used for prescreening of antimicrobial agents and for studies of host-pathogen interactions. in the present study, 17 lactobacillus isolates from chicken and pig intestines were tested with c. elegans, and the ability ...	2011	21219766
bactometer system versus traditional methods for monitoring bacteria populations in salchichón during its ripening process.	the performance of the bactometer system (an impedimetric microbial monitoring system) compared with traditional methods (microbial colony counts) for monitoring bacterial populations (lactic acid bacteria [lab], enterobacteriaceae, and coliforms) was studied in 90 samples of an experimental salchichón (a type of spanish ripened dry sausage) during its ripening process. the population quantitations were carried out with fresh sausage, semiripened sausage (14 days of ripening), and finished produ ...	2011	21219778
molecular and biochemical properties of the s-layer protein from the wine bacterium lactobacillus hilgardii b706.	different strains of the genus lactobacillus can be regularly isolated from must and wine samples. by various physiological activities, they can improve or reduce the wine quality. lactobacillus hilgardii that is known to survive under harsh wine conditions is classified as a spoilage bacterium, e.g. due to the production of histamine. many lactobacilli form an s-layer as the outermost cell wall component which has been found to facilitate the colonization of special ecological niches. a detaile ...	2011	21221529
antioxidant, antihypertensive, and immunomodulatory activities of peptide fractions from fermented skim milk with lactobacillus delbrueckii ssp. bulgaricus lb340.	the aim of this study was to evaluate the antioxidant, antihypertensive and immunomodulatory characteristics of skim milk fermented with lactobacillus delbrueckii ssp. bulgaricus lb340. supernatants obtained from the ferments after centrifugation were subjected to ultrafiltration and yielded four peptidic fractions of 10-5 kda, 5-3 kda, 3-1 kda, and <1â·0 kda. peptides in 5-3 kda range exhibited a good antioxidant activity. the peptides (<1â·0 k) was applied to superdex-30 g column fractionation ...	2011	21214965
impact of dietary counselling and probiotic intervention on maternal anthropometric measurements during and after pregnancy: a randomized placebo-controlled trial.	to establish whether probiotic supplemented dietary counselling influences maternal anthropometric measurements during and after pregnancy.	2011	20970896
effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers.	this study investigated the effects of dietary supplementation with the prebiotics fructo-oligosaccharide (fos) and mannan-oligosaccharide (mos) on the performance, small intestinal microflora, and immune response of broilers. two hundred forty 1-d-old ross broiler chickens were randomly assigned to 6 dietary treatment groups: control, avilamycin (6 mg/kg), 0.25% fos, 0.5% fos, 0.025% mos, and 0.05% mos. each treatment was fed to 4 replicates of 10 birds per diet for 4 wk. except for the 0.5% fo ...	2011	21177446
role of probiotics on gut permeability and endotoxemia in patients with acute pancreatitis: a double-blind randomized controlled trial.	to study the role of probiotics on gut permeability and endotoxemia in patients with acute pancreatitis (ap).	2011	21135704
pulse probiotic administration induces repeated small intestinal muc3 expression in rats.	upon ingestion, probiotics may act to protect the host through a number of protective mechanisms including modulation of genes involved in intestinal innate mucosal defense such as epithelial cell-derived mucin glycoproteins and inhibitor of apoptosis proteins. to determine the specificity of effect and sustainability of response in vivo, lactobacillus plantarum 299v (lp299v), lactobacillus rhamnosus r0011 (lrr0011), and bifidobacterium bifidum r0071 (bbr0071) were added repeatedly or intermitte ...	2011	21135754
improvement of l-lactic acid production by osmotic-tolerant mutant of lactobacillus casei at high temperature.	l-lactic acid production by lactobacillus casei was used as a model to study the mechanism of substrate inhibition and the strategy for enhancing l-lactic acid production. it was found that the concentration of cell growth and l-lactate decreased with the increase of glucose concentration and fermentation temperature. to enhance the osmotic stress resistance of the strain at high temperature, a mutant g-03 was screened and selected with 360 g/l glucose at 45°c as the selective criterion. to furt ...	2011	20857288
rapid evaluation of the antibiotic susceptibility of fuel ethanol contaminant biofilms.	bacterial contaminants from commercial fuel ethanol production facilities were previously shown to form biofilms as mixed cultures under laboratory conditions. in this study, a rapid assay was developed to simultaneously compare isolates for their ability to form biofilms as pure cultures. a total of 10 strains were isolated from a dry-grind fuel ethanol plant that routinely doses with virginiamycin. these were identified by sequence analysis as six strains of lactobacillus fermentum, two strain ...	2011	20855199
human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways.	probiotic bacteria, specific representatives of bacterial species that are a common part of the human microbiota, are proposed to deliver health benefits to the consumer by modulation of intestinal function through largely unknown molecular mechanisms. to explore in vivo mucosal responses of healthy adults to probiotics, we obtained transcriptomes in an intervention study after a double-blind placebo-controlled cross-over design. in the mucosa of the proximal small intestine of healthy volunteer ...	2011	20823239
effects of oral administration of lactobacillus acidophilus l-92 on the symptoms and serum markers of atopic dermatitis in children.	few studies have investigated the complementary effects of long-term oral administration of lactobacillus acidophilus on traditional medical therapy in the treatment of patients with atopic dermatitis (ad).	2011	20861645
perioperative synbiotic treatment to prevent infectious complications in patients after elective living donor liver transplantation: a prospective randomized study.	although the effect of synbiotic therapy using prebiotics and probiotics has been reported in hepatobiliary surgery, there are no reports of the effect on elective living-donor liver transplantation (ldlt).	2011	20619394
lactobacillus plantarum prevents the development of colitis in il-10-deficient mouse by reducing the intestinal permeability.	it is reported that defects exist in the small intestinal epithelial barrier of inflammatory bowel disease, which might be associated with increased intestinal permeability at a very early stage. our study aims to investigate the role of lactobacillus plantarum on the decrease of epithelial permeability and the further protective effects on the intestinal epithelial barrier using the il-10-deficient mouse model. our study showed that tight junction associated proteins were increased after the pr ...	2011	20571906
immobilization of a recombinant esterase from lactobacillus plantarum on polypropylene accurel mp1000.	a recombinant esterase from lactobacillus plantarum was immobilized on hydrophobic support polypropylene accurel mp1000 by adsorption. adsorption efficiency was 83%, and the immobilized protein was 12.4 mg/g of support. esterase activity was determined using p-nitrophenyl butyrate as substrate, and highest activities were observed at 50 °c for immobilized enzyme and 30 °c for free enzyme extract. concerning thermal stability, after enzyme incubation at 80 °c for 30 min, immobilized and free enzy ...	2011	20652764
oral administration of clostridium butyricum for modulating gastrointestinal microflora in mice.	this study aimed to evaluate the safety of clostridium butyricum and to investigate the effect of c. butyricum on mice ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from caecal contents. we firstly evaluated the safety of c. butyricum using acute toxicity test and ames test. then forty male balb/c mice were divided into the following four treatment groups, each consisting of ten mice: normal group, low-dose group, medium-dose group and ...	2011	20711781
analysis of human and animal fecal microbiota for microbial source tracking.	microbial compositions of human and animal feces from south korea were analyzed and characterized. in total, 38 fecal samples (14 healthy adult humans, 6 chickens, 6 cows, 6 pigs and 6 geese) were analyzed by 454 pyrosequencing of the v2 region of the 16s rrna gene. four major phyla, actinobacteria, proteobacteria, firmicutes and bacteroidetes, were identified in the samples. principal coordinate analysis suggested that microbiota from the same host species generally clustered, with the exceptio ...	2011	20686512
characterization of the cryptic plasmid ptxw from lactobacillus paracasei txw.	a cryptic plasmid from lactobacillus paracasei txw isolated from koumiss, designated as ptxw, was sequenced and characterized. it is 3178 bp in length with a g+c content of 42.9%. the plasmid ptxw was predicted to encode 4 putative orfs. repb shared high homology with initiator proteins of plasmids from the rolling circle replication (rcr) pmv158 family. copg was predicted to encode a transcriptional repressor, which was involved in plasmid copy number control together with a putative antisense ...	2011	20709099
the effect of exopolysaccharide-producing probiotic strains on gut oxidative damage in experimental colitis.	oxidative stress plays a role in disease initiation and progression in inflammatory bowel disease (ibd) and manipulation of this pathway may attenuate disease progress. in this study, the effect of exopolysaccharide (eps)-producing probiotic bacteria on gut oxidative damage was evaluated in a rat model of experimental colitis.	2011	20683661
in vitro oral biofilm formation on triclosan-coated sutures in the absence and presence of additional antiplaque treatment.	this study evaluated the in vitro plaque inhibitory effect of triclosan-coated polyglactin 910 sutures in the absence and presence of an additional antiplaque agent commonly used after oral surgery.	2011	20674122
splenic abscess caused by lactobacillus paracasei.	splenic abscess is relatively uncommon. infections caused by lactobacilli, which are usually one of the constituents of normal flora of human gut and vagina, are also very uncommon. here we report a case of splenic abscess caused by lactobacillus paracasei. we performed a literature review for this rare entity. immunocompromised status of the patient, who had uncontrolled diabetes, could have contributed to the pathogenesis of this rare disease.	2011	20669039
role of probiotic in preventing acute diarrhoea in children: a community-based, randomized, double-blind placebo-controlled field trial in an urban slum.	summaryacute diarrhoea remains a major public health challenge in developing countries. we examined the role of a probiotic in the prevention of acute diarrhoea to discover if there was an effect directed towards a specific aetiology. a double-blind, randomized, controlled field trial involving 3758 children aged 1-5 years was conducted in an urban slum community in kolkata, india. participants were given either a probiotic drink containing lactobacillus casei strain shirota or a nutrient drink ...	2011	20670468
glycemic index and phenolics of partially-baked frozen bread with sourdough.	different lactic acid bacteria starters were used to prepare sourdough to make partially-baked frozen wholemeal wheat bread. the sourdough was prepared with a pure culture of lactobacillus plantarum or with commercial starters containing lactobacillus brevis combined with saccharomyces cerevisiae var. chevalieri (lv4), lactobacillus fermentum (pl1), or lactobacillus fermentum with phytase (pl3). we determined the acetic and lactic acid concentrations in sourdough, bread chemical composition, tot ...	2011	20715901
sensitive lactate determination based on acclimated mixed bacteria and palygorskite co-modified oxygen electrode.	a sensitive bacteria biosensor was prepared for the detection of trace lactate. the sensitive bioelement, lactobacillus bulgaricus and streptococcus thermophilus mixed culture, and palygorskite, a perfect matrix for bacteria, was co-immobilized on the surface of oxygen electrode. the biosensor possesses fine selective specificity, good sensitivity and longer operational life time, which were due to the mutual help relationship of symbiotic bacteria and 240 days acclimation with lactate as the ca ...	2011	20724225
microfiltration conditions modify lactobacillus bulgaricus cryotolerance in response to physiological changes.	this work aimed at analyzing the effect of microfiltration conditions (cross-flow velocity and transmembrane pressure) on the quality of frozen lactobacillus bulgaricus cfl1 starters produced on pilot scale. microfiltered cells were less resistant during the concentration process than centrifuged cells. in contrast, bacterial cryotolerance during freezing was improved after microfiltration, in a range of 28-88%, depending on the microfiltration conditions. during frozen storage, cell resistance ...	2011	20803034
pisciglobus halotolerans gen. nov., sp. nov., isolated from fish sauce.	two strains of gram-stain-positive, catalase-negative, tetrad-forming cocci, c01(t) and c02, were isolated in thailand from fish sauce. they were facultatively anaerobic, non-motile and non-spore-forming bacteria. these strains produced l-lactic acid from glucose. they grew at ph 5.0-9.0, at 15-40 °c and in the presence of 10 % (w/v) nacl. the dominant fatty acid was c(18 : 1)ω9c. the dna g+c contents of strains c01(t) and c02 were 38.6 and 38.7 mol%, respectively. strain c01(t) was related most ...	2011	20729308
probiotics versus antibiotic decontamination of the digestive tract: infection and mortality.	selective decontamination of the digestive tract (sdd) has been shown to decrease the infection rate and mortality in intensive care units (icus); lactobacillus plantarum 299/299v plus fibre (lab) has been used for infection prevention and does not harbour the potential disadvantages of antibiotics. the objective was to assess whether lab is not inferior to sdd in infection prevention.	2011	20721536
the dnak gene as a molecular marker for the classification and discrimination of the lactobacillus casei group.	it is hard to accurately identify specific species of the lactobacillus casei group using phenotypic techniques alone. some strains of this species group are considered to be probiotic and are widely applied in the food industry. in this study, we compared the use of two phylogenetic markers, the 16s rrna and dnak genes, for species discrimination of the members of the l. casei group using sequencing and rflp. the results showed that l. casei, lactobacillus paracasei, lactobacillus zeae and lact ...	2011	20700765
lactobacillus rhamnosus gr-1 stimulates colony-stimulating factor 3 (granulocyte) (csf3) output in placental trophoblast cells in a fetal sex-dependent manner.	bacterial vaginosis is associated with a 1.4-fold increased risk of preterm birth. we have shown previously that lactobacillus rhamnosus gr-1 supernatant up-regulates interleukin 10 and down-regulates tumor necrosis factor-alpha output in lipopolysaccharide (lps)-treated human primary placenta cultures in a fetal sex-dependent manner. we hypothesize that lactobacilli also exert their anti-inflammatory effect by up-regulation of colony-stimulating factor 3 (granulocyte) (csf3), which is secreted ...	2011	20811016
effect of freeze-drying on viability and in vitro probiotic properties of a mixture of lactic acid bacteria and yeasts isolated from kefir.	the effect of freeze-drying on viability and probiotic properties of a microbial mixture containing selected bacterial and yeast strains isolated from kefir grains (lactobacillus kefir, lactobacillus plantarum, lactococcus lactis, saccharomyces cerevisiae and kluyveromyces marxianus) was studied. the microorganisms were selected according to their potentially probiotic properties in vitro already reported. two types of formulations were performed, a microbial mixture (mm) suspended in milk and a ...	2011	20822567
the synergism of natural compounds in the pursuit of safe and healthier food.	food producers apply modern processing techniques and use a variety of preservative additives to guarantee safe food and a longer shelflife. regrettably many of these impact the sensory characteristics of the foodstuffs, such as colour, texture, and flavour, which can result in low consumer acceptance. additionally, strategies used to reduce growth of spoilage and pathogenic bacteria are not selective enough and may inactivate also desired microbiota. food is usually overdosed with antimicrobial ...	2011	20811804
rectal administration of lactobacillus casei dg modifies flora composition and toll-like receptor expression in colonic mucosa of patients with mild ulcerative colitis.	an imbalance in gut microbiota seems to contribute to the development of chronic inflammatory disorders of the gastrointestinal tract, such as ulcerative colitis (uc). although it has been suggested that probiotic supplementation is an effective approach to colitis, its effects on intestinal flora and on mucosal cytokine balance have never been explored.	2011	20737210
randomised, double-blind and placebo-controlled study using new probiotic lactobacilli for strengthening the body immune defence against viral infections.	the aim of this study was to investigate whether consumption of lactobacillus plantarum heal 9 (dsm 15312) and lactobacillus paracasei 8700:2 (dsm 13434) could affect naturally acquired common cold infections in healthy subjects.	2011	20803023
new perspectives for lactobacilli exopolysaccharides.	lactobacilli have the ability to produce different kinds of exopolysaccharides (eps) exhibiting a wide diversity of structures. eps are classified, according to their composition into homopolysaccharides and heteropolysaccharides. one of their most described applications is their utilization as texturing agents naturally synthesized in the fermented food products. nowadays, in regard to demand of modern consumers focusing towards safe and healthy food without additives, new perspectives of devel ...	2011	20807563
mannitol production by lactic acid bacteria grown in supplemented carob syrup.	detailed kinetic and physiological characterisation of eight mannitol-producing lactic acid bacteria, leuconostoc citreum atcc 49370, l. mesenteroides subsp. cremoris atcc19254, l. mesenteroides subsp. dextranicum atcc 19255, l. ficulneum nrrl b-23447, l. fructosum nrrl b-2041, l. lactis atcc 19256, lactobacillus intermedius nrrl 3692 and lb. reuteri dsm 20016, was performed using a carob-based culture medium, to evaluate their different metabolic capabilities. cultures were thoroughly followed ...	2011	20820868
diversity of intestinal bifidobacteria in patients with japanese cedar pollinosis and possible influence of probiotic intervention.	this study was conducted to evaluate the potential association between intestinal bifidobacteria and japanese cedar pollinosis (jcpsis) and possible influences of probiotic intervention. in this study, fecal samples were the collected from 29 jcpsis patients. the qualitative and quantitative analyses of fecal bifidobacteria were conducted by quantitative real-time pcr with 16s rrna-gene-targeted species-specific primers before cedar pollen spread and after a 10-week intervention with fermented m ...	2011	20512497
lactobacillus kimchicus sp. nov., a β-glucosidase-producing bacterium isolated from kimchi.	a gram-reaction-positive, non-spore-forming, rod-shaped, non-motile, β-glucosidase-producing bacterium, designated dcy51(t), was isolated from kimchi, a korean fermented vegetable food. 16s rrna gene sequence analysis revealed that strain dcy51(t) belonged to the genus lactobacillus and exhibited highest 16s rrna gene sequence similarity with lactobacillus paracollinoides ab 74 (96.9 %), l. similis jcm 2765(t) (96.9 %), l. collinoides jcm 1123(t) (96.7 %) and l. hilgardii dsm 20176(t) (95.5 %). ...	2011	20495030
the effect of starch gelatinisation degree on intestinal morphology, intestinal ph and bacteriology in pigeons.	the effect of starch gelatinisation degree in extruded feed on intestinal morphology, intestinal ph and faecal bacteriology was investigated in pigeons. extruded complete pigeon diets would offer the principle advantage of providing equilibrated nutrients and energy, but factors such as starch gelatinisation require investigation before these diets are offered as main dietary items to pigeons. birds were fed two diets with equal ingredient composition and nutrient content, but with a different d ...	2011	20487096
vaginal microbiome of reproductive-age women.	the means by which vaginal microbiomes help prevent urogenital diseases in women and maintain health are poorly understood. to gain insight into this, the vaginal bacterial communities of 396 asymptomatic north american women who represented four ethnic groups (white, black, hispanic, and asian) were sampled and the species composition characterized by pyrosequencing of barcoded 16s rrna genes. the communities clustered into five groups: four were dominated by lactobacillus iners, l. crispatus, ...	2011	20534435
functional characteristics of lactobacillus fermentum f1.	in this study, lactobacillus fermentum (l. fermentum) f1 reduced cholesterol 48.87%. the strain was screened from cattle feces using an api 50 chl system and the 16s rrna sequence contrasting method. l. fermentum f1 showed acid and bile tolerance, and antimicrobial activity against pathogenic escherichia coli and staphylococcus aureus. l. fermentum f1 deconjugated 0.186 mm of free cholalic acid after it was incubated at 37°c in 0.20% sodium taurocholate (tca) broth for 24 h. heat-killed and rest ...	2011	20490496
lactobacillus koreensis sp. nov., isolated from the traditional korean food kimchi.	a lactic acid bacterium, strain dcy50(t), isolated from the traditional korean food kimchi, was studied to determine its taxonomic position. the strain was gram-stain-positive, catalase-negative, facultatively anaerobic, rod-shaped and motile. the genomic dna g+c content was 49 mol% and the peptidoglycan structure was of the a4α (l-lys-d-asp) type. chemotaxonomic markers of the strain were consistent with its classification in the genus lactobacillus. comparisons of 16s rrna and rpoa gene sequen ...	2011	20435754
repeated fed-batch lactic acid production in a packed bed-stirred fermentor system using a ph feedback feeding method.	lactic acid production by repeated fed-batch fermentation using free and immobilized cells of lactobacillus lactis-11 in a packed bed-stirred fermentor (pbsf) system filled with different support materials including ceramic beads, macro-activated carbon cylinders and glass fiber balls was investigated. the results showed that the optimal support materials were the ceramic beads with diameters of 1-2 mm. compared with the free cell fermentation system, lactic acid production and volumetric produc ...	2011	20607299
preparation of selenium/zinc-enriched probiotics and their effect on blood selenium and zinc concentrations, antioxidant capacities, and intestinal microflora in canine.	the aim of this study was to prepare se/zn-enriched probiotics and investigate their effect on blood se and zn concentrations, blood antioxidant capacities, and intestinal microflora in canine. the preparation was performed in a single-factor experiment. the optimal culture conditions were as follows: the initial concentrations of se(4+) and zn(2+) were 5 and 150 µg/ml, respectively; the inoculation volume was 5%; and the liquid volume of the medium was 50 ml in a 250-ml flask. the medium was th ...	2011	20563665
effects of liquid metabolite combinations produced by lactobacillus plantarum on growth performance, faeces characteristics, intestinal morphology and diarrhoea incidence in postweaning piglets.	a study was carried out to investigate the effects of feeding liquid metabolite combinations produced by lactobacillus plantarum strains on growth performance, diarrhoea incidence, faecal ph, microfloral counts, short-chain fatty acids (scfa) and intestinal villus height and crypt depth of postweaning piglets. a total of 120 piglets (26 days old) were randomly assigned evenly into five treatment groups treated with same basal diet: (1) -ve control (free antibiotic); (2) + ve control (0.03% of ch ...	2011	20632092
antimicrobial activity of terpenoids from copaifera langsdorffii desf. against cariogenic bacteria.	in the present work, the anticariogenic activities of nine labdane type-diterpenes and four sesquiterpenes were investigated. among these metabolites, (-)-copalic acid (ca) was the most active compound displaying mic values very promising (ranging from 2.0 to 6.0 µg/ml) against the main microorganisms responsible for dental caries: streptococcus salivarius, s. sobrinus, s. mutans, s. mitis, s. sanguinis and lactobacillus casei. time kill assays performed with ca against the primary causative age ...	2011	20632306
host-microbial symbiosis in the vertebrate gastrointestinal tract and the lactobacillus reuteri paradigm.	vertebrates engage in symbiotic associations with vast and complex microbial communities that colonize their gastrointestinal tracts. recent advances have provided mechanistic insight into the important contributions of the gut microbiome to vertebrate biology, but questions remain about the evolutionary processes that have shaped symbiotic interactions in the gut and the consequences that arise for both the microbes and the host. here we discuss the biological principles that underlie microbial ...	2011	20615995
bioconversion of renewable resources into lactic acid: an industrial view.	lactic acid, an anaerobic product of glycolysis, can be theoretically produced by synthetic route; however, it is commercially produced by homo-fermentative batch mode of operations. factors affecting its production and strategies improving it are considered while devising an optimized protocol. although a hetero-fermentative mode of production exists, it is rarely used for commercial production. attempts to use rhizopus sp. for lactic acid production through either hetero-fermentative or thermo ...	2011	20476870
cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract.	cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ...	2011	20488844
probiotics for patients with compensated liver cirrhosis: a double-blind placebo-controlled study.	gut flora is related to the major complications of liver cirrhosis including hepatic encephalopathy, spontaneous bacterial peritonitis, and variceal bleeding. prior studies have reported a beneficial effect of gut flora modification with probiotic bacteria in patients with minimal hepatic encephalopathy. we aimed to study the effect of probiotics on clinical and laboratory parameters of patients with compensated cirrhosis.	2011	20452184
role of probiotics in the prevention of the enteric colonization by candida in preterm newborns: incidence of late-onset sepsis and neurological outcome.	to evaluate the efficacy of probiotics in the prevention of gastrointestinal colonization by candida species, of late-onset sepsis and neurological outcome in preterm newborns.	2011	20410904
characterization of a bacteriocin produced by lactobacillus sakei r1333 isolated from smoked salmon.	strain r1333, isolated from commercially available smoked salmon, was identified as lactobacillus sakei based on biochemical tests, sugar fermentation reactions (api 50 chl), pcr with species-specific primers and sequencing of the 16s rrna gene. strain r1333 produces a 3811 kda class iia bacteriocin, active against streptococcus caprinus, streptococcus macedonicus, streptococcus spp., l. sakei, lactococcus lactis subsp. lactis, listeria innocua, listeria ivanovii subsp. ivanovii and listeria mon ...	2011	20152920