Publications

homogeneous assay of target molecules based on chemiluminescence resonance energy transfer (cret) using dnazyme-linked aptamers.	we have designed a single-stranded dnazyme-aptamer sensor for homogeneous target molecular detection based on chemiluminescence resonance energy transfer (cret). the structure of the engineered single-stranded dna (ssdna) includes the horseradish peroxidase (hrp)-like dnazyme, optimum-length linker (10-mer-length dna), and target-specific aptamer sequences. a quencher dye was modified at the 3' end of the aptamer sequence. the incorporation of hemin into the g-quadruplex structure of dnazyme yie ...	2014	24658027
development of enzyme-linked immunosorbent assay for estimation of urinary albumin.	yearly estimation of urinary albumin is a prerequisite for predicting renal status in diabetes type ii patients with negative dipstick results for overt proteinuria. a simple, sensitive, and cost-effective enzyme linked immunosorbent assay (elisa) for urinary albumin has been developed using human serum albumin antiserum (hsa-antiserum), hsa-biotin, and streptavidin-horseradish peroxidase (sa-hrp) conjugates. to the antibody-coated wells, 100 μl of hsa standards followed by 1:100 diluted urine s ...	2014	24654825
thin-layer polymer wrapped enzymes encapsulated in hierarchically mesoporous silica with high activity and enhanced stability.	a novel soft-hard cooperative approach was developed to synthesize bioactive mesoporous composite by pre-wrapping penicillin g amidase with poly(acrylaimde) nanogel skin and subsequently incorporating such penicillin g amidase nanocapsules into hierarchically mesoporous silica. the as-received bioactive mesoporous composite exhibited comparable activity and extraordinarily high stability in comparison with native penicillin g amidase and could be used repetitively in the water-medium hydrolysis ...	2014	24651701
expression of human apolipoprotein e4 reduces insulin-receptor substrate 1 expression and akt phosphorylation in the ageing liver.	the diabetic drug rosiglitazone was reported to improve glucose tolerance in insulin-resistant apoe3 but not apoe4 knock-in mice. we therefore examined whether apolipoprotein e (apoe) has genotype-specific effects on liver insulin function. at 12 weeks, no difference in liver insulin signaling was detected between fasting apoe3 and apoe4 mice. at 72 weeks however, apoe4 mice had lower irs-1 and pi3k expression, and reduced akt phosphorylation. this decline was associated with lower insulin and h ...	2014	24649407
ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum.	ursolic acid (3β-hydroxy-urs-12-en-28-oic acid) is a natural pentacyclic triterpenoid that is present in many plants, including medicinal herbs, and foods. ursolic acid was initially identified as an inhibitor of the expression of intercellular adhesion molecule-1 (icam-1) in response to interleukin-1α (il-1α). we report here a novel biological activity: ursolic acid inhibits intracellular trafficking of proteins. ursolic acid markedly inhibited the il-1α-induced cell-surface icam-1 expression i ...	2014	24649404
supramolecular assemblies of nucleoside functionalized carbon nanotubes: synthesis, film preparation, and properties.	nucleoside-functionalized multi-walled carbon nanotubes (n-mwcnts) were synthesized and characterized. a self-organization process using hydrogen bonding interactions was then used for the fabrication of self-assembled n-mwcnts films free of stabilizing agents, polymers, or surfactants. membranes were produced by using a simple water-dispersion-based vacuum-filtration method. hydrogen-bond recognition was confirmed by analysis with ir spectroscopy and tem images. restoration of the electronic co ...	2014	24648250
a dna origami nanorobot controlled by nucleic acid hybridization.	a prototype for a dna origami nanorobot is designed, produced, and tested. the cylindrical nanorobot (diameter of 14 nm and length of 48 nm) with a switchable flap, is able to respond to an external stimulus and reacts by a physical switch from a disarmed to an armed configuration able to deliver a cellular compatible message. in the tested design the robot weapon is a nucleic acid fully contained in the inner of the tube and linked to a single point of the internal face of the flap. upon actuat ...	2014	24648163
[irreversible chemical afm-fishing for the detection of low-copied proteins].	the atomic-force microscopy-based method of irreversible chemical afm-fishing (afm-if(ch)) has been developed for the detection of proteins at ultra-low concentrations in solution. using this method, a very low concentration of horseradish peroxidase (hrp) protein (10(-17) m) was detected in solution. a theoretical model that allows the description of obtained experimental data, is proposed. this model takes into consideration both the transport of the protein from the bulk solution onto the afm ...	2014	24749246
rapid screening of multiple antibiotic residues in milk using disposable amperometric magnetosensors.	disposable amperometric magnetosensors, involving a mixture of modified-magnetic beads (mbs), for the multiplex screening of cephalosporins (cphs), sulfonamides (sas) and tetracyclines (tcs) antibiotic residues in milk are reported for the first time in this work. the multiplexed detection relies on the use of a mixture of target specific modified magnetic beads (mbs) and application of direct competitive assays using horseradish peroxidase (hrp)-labeled tracers. the amperometric responses measu ...	2014	24745735
purification and characterization of novel cationic peroxidases from asparagus acutifolius l. with biotechnological applications.	four novel basic peroxidases, named aap-1, aap-2, aap-3, and aap-4, were purified from asparagus acutifolius l. seeds by cation-exchange and gel filtration chromatographies. the four proteins showed a similar electrophoretic mobility of 46 kda while, by maldi-tof ms, different mr values of 42758.3, 41586.9, 42796.3, and 41595.5 were determined for aap-1, aap-2, aap-3, and aap-4, respectively. n-terminal sequences of aaps 1-4 up to residue 20 showed a high percentage of identity with the peroxida ...	2014	24740695
macro/nano-gel composite as an injectable and bioactive bulking material for the treatment of urinary incontinence.	many women around the world are suffering from urinary incontinence, defined as the unintentional leakage of urine by external abnormal pressure. although various kinds of materials have been utilized to treat this disease, therapies that are more effective are still needed for the treatment of urinary incontinence. here, we present a macro/nanogel composed of in situ forming gelatin-based macrogels and self-assembled heparin-based nanogels, which can serve as an injectable and bioactive bulking ...	2014	24739122
whisker-related circuitry in the trigeminal nucleus principalis: ultrastructure.	trigeminal (v) nucleus principalis (prv) is the requisite brainstem nucleus in the whisker-to-barrel cortex model system that is widely used to reveal mechanisms of map formation and information processing. yet, little is known of the actual prv circuitry. in the ventral "barrelette" portion of the adult mouse prv, relationships between v primary afferent terminals, thalamic-projecting prv neurons, and gamma-aminobutyric acid (gaba)-ergic terminals were analyzed in the electron microscope. prima ...	2014	24738912
simultaneous electropolymerization and electro-click functionalization for highly versatile surface platforms.	simple preparation methods of chemically versatile and highly functionalizable surfaces remain rare and present a challenging research objective. here, we demonstrate a simultaneous electropolymerization and electro-click functionalization process (seec) for one-pot self-construction of aniline- and naphthalene-based functional polymer films where both polymerization and click functionalization are triggered by applying electrochemical stimuli. cyclic voltammetry (cv) can be applied for the simu ...	2014	24738664
synergy-based small-molecule screen using a human lung epithelial cell line yields δf508-cftr correctors that augment vx-809 maximal efficacy.	the most prevalent cystic fibrosis transmembrane conductance regulator (cftr) mutation causing cystic fibrosis, δf508, impairs folding of nucleotide binding domain (nbd) 1 and stability of the interface between nbd1 and the membrane-spanning domains. the interfacial stability defect can be partially corrected by the investigational drug vx-809 (3-[6-[[[1-(2,2-difluoro-1,3-benzodioxol-5-yl)cyclopropyl]carbonyl]amino]-3-methyl-2-pyridinyl]-benzoic acid) or the r1070w mutation. second-generation δf ...	2014	24737137
evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs.	bifocal distraction osteogenesis has been shown to be a reliable method for reconstructing segmental mandibular defects. however, there are few reports regarding the occurrence of inferior alveolar nerve regeneration during the process of distraction. previously, we reported inferior alveolar nerve regeneration after distraction, and evaluated the regenerated nerve using histological and electrophysiological methods. in the present study, we investigated axons regenerated by bifocal distraction ...	2014	24732938
a rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk.	an immunomagnetic beads-based enzyme-linked immunosorbent assay (imbs-elisa) was developed for the detection of β-casein in bovine milk. immunomagnetic beads (imbs) were employed as the solid phase. the anti-β-casein monoclonal antibody (mcab) bound to imbs was used as capture probe and an anti-β-casein polyclonal antibody (pcab), labelled with horseradish peroxidase (hrp), was employed as detector probe. three reaction and two washing steps were needed. each reaction needed 10 min or less, whic ...	2014	24731368
development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin m1 in milk.	a direct competitive chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) for detecting aflatoxin m1 (afm1) was developed. to improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (sptz) and 4-morpholinopyridine (morph) was used to enhance peroxidase-induced cl. the concentrations of the coating anti-afm1 antibody and the conjugate of afb1 with horseradish peroxidase the conditions of the chemiluminescent assay were varied to optimise the conditio ...	2014	24731347
highly sensitive microfluidic competitive enzyme immunoassay based on chemiluminescence resonance energy transfer for the detection of neuron-specific enolase.	a microfluidic competitive enzyme immunoassay based on chemiluminescence resonance energy transfer (cret) was developed for highly sensitive detection of neuron-specific enolase (nse). the cret system consisted of horseradish peroxidase (hrp)/luminol as a light donor and fluorescein isothiocyanate as an acceptor. when fluorescein isothiocyanate-labeled antibody binds with hrp-labeled antigen to form immunocomplex, the donor and acceptor are brought close each other and cret occurs in the immunoc ...	2014	24723253
enzyme-based inverse opals: a facile and promising platform for fabrication of biocatalysts.	a facile and promising approach was developed to fabricate enzyme-based 3d-ordered macroporous biocatalysts (enzyme-based inverse opals) by using the colloidal crystal templating method. horseradish peroxidase- and amylase-based inverse opals were prepared, which verified that this method is suitable for various enzymes.	2014	24722982
an optical sensor for pesticide determination based on the autoindicating optical properties of peroxidase.	during the enzymatic reaction of the heme-protein horseradish peroxidase (hrp) with hydrogen peroxide there are changes in the molecular absorption spectra of hrp and its different oxidation states which can be used for quantitative determination of the substrate. one of these intermediate oxidation states is the hrpii, with iron as an oxyferryl. this compound is assumed to be responsible for the organophosphate pesticide degradation in the fenton reaction. in this work, the enzymatic hrp-h₂o₂ r ...	2014	24720992
electrochemical immunosensor based on hydrophilic polydopamine-coated prussian blue-mesoporous carbon for the rapid screening of 3-bromobiphenyl.	a sensitive electrochemical immunosensor for 3-bromobiphenyl (3-bbp) detection was constructed by employing a new polydopamine coated prussian blue-mesoporous carbon (pdop/pb/cmk-3) nanocomposite as the substrate platform and multi-horseradish peroxidase-double helix carbon nanotubes-secondary antibody (multi-hrp-dhcnts-ab2) as the signal label. pb/cmk-3 was firstly successfully in-situ synthesized with the aid of the cmk-3 reduction, which was characterized by transmission electron microscope ( ...	2014	24709325
central pupillary light reflex circuits in the cat: i. the olivary pretectal nucleus.	the central pathways subserving the feline pupillary light reflex were examined by defining retinal input to the olivary pretectal nucleus (opt), the midbrain projections of this nucleus, and the premotor neurons within it. unilateral intravitreal wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) injections revealed differences in the pattern of retinal opt termination on the two sides. injections of wga-hrp into opt labeled terminals bilaterally in the anteromedian nucleus, and ...	2014	24706328
mechanism and regulation of peroxidase-catalyzed nitric oxide consumption in physiological fluids: critical protective actions of ascorbate and thiocyanate.	catalytic consumption of nitric oxide (no) by myeloperoxidase and related peroxidases is implicated as playing a key role in impairing no bioavailability during inflammatory conditions. however, there are major gaps in our understanding of how peroxidases consume no in physiological fluids, in which multiple reactive enzyme substrates and antioxidants are present. notably, ascorbate has been proposed to enhance myeloperoxidase-catalyzed no consumption by forming no-consuming substrate radicals. ...	2014	24704973
dna sensors and aptasensors based on the hemin/g-quadruplex-controlled aggregation of au nps in the presence of l-cysteine.	l-cysteine induces the aggregation of au nanoparticles (nps), resulting in a color transition from red to blue due to interparticle plasmonic coupling in the aggregated structure. the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme catalyzes the aerobic oxidation of l-cysteine to cystine, a process that inhibits the aggregation of the nps. the degree of inhibition of the aggregation process is controlled by the concentration of the dnazyme in the system. these functions are implement ...	2014	24700798
enzyme encapsulated hollow silica nanospheres for intracellular biocatalysis.	hollow silica nanospheres (hsn) with low densities, large interior spaces and permeable silica shells are suitable for loading enzymes in the cavity to carry out intracellular biocatalysis. the porous shell can protect the encapsulated enzymes against proteolysis and attenuate immunological response. we developed a microemulsion-templating method for confining horseradish peroxidase (hrp) in the cavity of hsn. this simple one-pot enzyme encapsulation method allows entrapping of the enzyme, which ...	2014	24694065
hierarchical hybrid peroxidase catalysts for remediation of phenol wastewater.	we report a new family of hierarchical hybrid catalysts comprised of horseradish peroxidase (hrp)-magnetic nanoparticles for advanced oxidation processes and demonstrate their utility in the removal of phenol from water. the immobilized hrp catalyzes the oxidation of phenols in the presence of h2 o2 , producing free radicals. the phenoxy radicals react with each other in a non-enzymatic process to form polymers, which can be removed by precipitation with salts or condensation. the hybrid peroxid ...	2014	24692298
sensitive electrochemical aptamer biosensor for dynamic cell surface n-glycan evaluation featuring multivalent recognition and signal amplification on a dendrimer-graphene electrode interface.	we demonstrate a multivalent recognition and highly selective aptamer signal amplification strategy for electrochemical cytosensing and dynamic cell surface n-glycan expression evaluation by the combination of concanavalin a (con a), a mannose binding protein, as a model, conjugated poly(amidoamine) dendrimer on a chemically reduced graphene oxide (rgo-den) interface, and aptamer- and horseradish peroxidase-modified gold nanoparticles (hrp-aptamer-aunps) as nanoprobes. in this strategy, the rgo- ...	2014	24684138
expression of maspin in benign and malignant salivary gland tumors: an immunohistochemical study.	maspin is a novel serine protease inhibitor (serpin) with multifaceted tumor-suppressive activities. it was originally identified in normal human breast myoepithelial cells and shows variable expression in different types of cancer cells. maspin displays anti-metastatic properties in mammary and prostate cancer. its expression is maintained during ovarian, lung and pancreatic carcinogenesis, indicating that maspin regulated metastatic potential is tissue specific. thus, it is possible that maspi ...	2014	25098993
fe-taml encapsulated inside mesoporous silica nanoparticles as peroxidase mimic: femtomolar protein detection.	peroxidase, such as horseradish peroxidase (hrp), conjugated to antibodies are routinely used for the detection of proteins via an elisa type assay in which a critical step is the catalytic signal amplification by the enzyme to generate a detectable signal. synthesis of functional mimics of peroxidase enzyme that display catalytic activity which far exceeds the native enzyme is extremely important for the precise and accurate determination of very low quantities of proteins (fm and lower) that i ...	2014	25089579
tyramine-based enzymatic conjugate repeats for ultrasensitive immunoassay accompanying tyramine signal amplification with enzymatic biocatalytic precipitation.	a new impedimetric immunoassay protocol based on enzyme-triggered formation of tyramine-enzyme repeats on gold nanoparticle (aunp) was designed for highly sensitive detection of carcinoembryonic antigen (cea, as a model) by virtue of utilizing enzymatic biocatalytic precipitation toward 4-chloro-1-naphthol (4-cn) on anti-cea antibody (ab1)-modified immunosensor. initially, aunp was functionalized with horseradish peroxidase and detection antibody (hrp-aunp-ab2), and then hrp-tyramine conjugate w ...	2014	25088522
excited singlet molecular o₂(¹δg) is generated enzymatically from excited carbonyls in the dark.	in mammalian tissues, ultraweak chemiluminescence arising from biomolecule oxidation has been attributed to the radiative deactivation of singlet molecular oxygen [o2 ((1)δg)] and electronically excited triplet carbonyl products involving dioxetane intermediates. herein, we describe evidence of the generation of o2 ((1)δg) in aqueous solution via energy transfer from excited triplet acetone. this involves thermolysis of 3,3,4,4-tetramethyl-1,2-dioxetane, a chemical source, and horseradish peroxi ...	2014	25087485
poly(neutral red) based hydrogen peroxide biosensor for chromium determination by inhibition measurements.	amperometric hydrogen peroxide enzyme inhibition biosensors based on horseradish peroxidase (hrp) immobilised on electropolymerised neutral red (nr) or directly on the surface of carbon film electrodes (cfe) have been successfully applied to the determination of toxic cr(iii) and cr(vi). parameters influencing the performance of the biosensor including the enzyme immobilisation method, the amount of hydrogen peroxide, applied potential and electrolyte ph were optimised. the inhibition of horsera ...	2014	25080156
π-conjugated carbon radicals at graphene oxide to initiate ultrastrong chemiluminescence.	graphene oxide has widely been employed in various fields, but its structure and composition has still not been fully understood. here we report that freshly prepared graphene oxide exhibits a large number of π-conjugated carbon radicals at its π-network plane, which result from the addition reaction of hydroxyl radicals from h2o2 onto the conjugated double bonds of graphene oxide. the π-conjugated carbon radicals can directly initiate the long-lasting visible chemiluminescence of luminol, which ...	2014	25078281
detecting the oxidative reactivity of nanoparticles: a new protocol for reducing artifacts.	understanding the oxidative reactivity of nanoparticles (nps; <100 nm) could substantially contribute to explaining their toxicity. we attempted to refine the use of 2'7-dichlorodihydrofluorescein (dcfh) to characterize np generation of reactive oxygen species (ros). several fluorescent probes have been applied to testing oxidative reactivity, but despite dcfh being one of the most popular for the detection of ros, when it has been applied to nps there have been an unexplainably wide variability ...	2014	25076842
hydrogen peroxide-independent generation of superoxide by plant peroxidase: hypotheses and supportive data employing ferrous ion as a model stimulus.	when plants are threaten by microbial attacks or treated with elicitors, alkalization of extracellular space is often induced and thus ph-dependent extracellular peroxidase-mediated oxidative burst reportedly takes place, especially at the site of microbial challenge. however, direct stimulus involved in activation of peroxidase-catalyzed oxidative burst has not been identified to date. here, we would like to propose a likely role for free ferrous ion in reduction of ferric native peroxidase int ...	2014	25071789
highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol.	a novel immunochromatographic assay (ica) was proposed for rapid and multiple assay of β2-agonists, by utilizing ractopamine (rac) and salbutamol (sal) as the models. owing to the introduction of chemiluminescent (cl) approach, the proposed protocol shows much higher sensitivity. in this work, the described ica was based on a competitive format, and horseradish peroxidase-tagged antibodies were used as highly sensitive cl probes. quantitative analysis of β2-agonists was achieved by recording the ...	2014	25066723
nerve growth factor promotes expression of novel genes in intervertebral disc cells that regulate tissue degradation: laboratory investigation.	increased neurotrophin activity in degenerative intervertebral discs (ivds) is one potential cause of chronic low-back pain (lbp). the aim of the study was to assess if nerve growth factor (ngf) might alter gene expression of ivd cells and contribute to disc degeneration by enhancing expression or activity of factors that cause breakdown of ivd matrix.	2014	25062286
glucosinolate profile and distribution among plant tissues and phenological stages of field-grown horseradish.	profile and distribution of glucosinolates (gls) were detected in plant tissues of horseradish at different developmental stages: beginning of vegetative re-growth, flowering and silique formation. the gls profile varied widely in the different tissues: we identified 17 gls in roots and sprouts, one of which was not previously characterized in horseradish, i.e. the 2(s)-hydroxy-2-phenylethyl-gls (glucobarbarin) and/or 2(r)-hydroxy-2-phenylethyl-gls (epiglucobarbarin), 11 already found in the roo ...	2014	25060759
g-quadruplex dna biosensor for sensitive visible detection of genetically modified food.	in this paper, a novel label-free g-quadruplex dnazyme sensor has been proposed for colorimetric identification of gmo using camv 35s promoter sequence as the target. the binary probes can fold into g-quadruplex structure in the presence of dna-t (target dna) and then combine with hemin to form a dnazyme resembling horseradish peroxidase. the detection system consists of two g-rich probes with 2:2 split mode by using the absorbance and color of abts(2-) as signal reporter. upon the addition of a ...	2014	25059184
roles of horseradish peroxidase in response to terbium stress.	the pollution of the environment by rare earth elements (rees) causes deleterious effects on plants. peroxidase plays important roles in plant response to various environmental stresses. here, to further understand the overall roles of peroxidase in response to ree stress, the effects of the ree terbium ion (tb(3+)) on the peroxidase activity and h2o2 and lignin contents in the leaves and roots of horseradish during different growth stages were simultaneously investigated. the results showed tha ...	2014	25055927
investigation of processes in black tea manufacture through model fermentation (oxidation) experiments.	flavanol depleted whole fresh green tea leaf powder, as reported in the literature, was used as matrix for a systematic study of the endogenous oxidative enzymatic conversion of selected flavanol combinations to theaflavins and thearubigins. the activity of the two crucial enzymes polyphenol oxidase (ppo) and peroxidase (pod) was controlled individually through addition of h2o2 and/or o2. using the endogenous peroxidase only it was shown that (-)-epicatechin alone did not react with pod. accordi ...	2014	25051300
using s-adenosyl-l-homocysteine capture compounds to characterize s-adenosyl-l-methionine and s-adenosyl-l-homocysteine binding proteins.	s-adenosyl-l-methionine (sam) is recognized as an important cofactor in a variety of biochemical reactions. as more proteins and pathways that require sam are discovered, it is important to establish a method to quickly identify and characterize sam binding proteins. the affinity of s-adenosyl-l-homocysteine (sah) for sam binding proteins was used to design two sah-derived capture compounds (ccs). we demonstrate interactions of the proteins comt and sahh with sah-cc with biotin used in conjuncti ...	2014	25172130
highly sensitive electrochemical aptasensor for immunoglobulin e detection based on sandwich assay using enzyme-linked aptamer.	here, we describe the fabrication of an electrochemical immunoglobulin e (ige) aptasensor using enzyme-linked aptamer in the sandwich assay method and thionine as redox probe. in this protocol, 5'-amine-terminated ige aptamer and thionine were covalently attached on glassy carbon electrode modified with carbon nanotubes/ionic liquid/chitosan nanocomposite. furthermore, another ige aptamer was modified with biotin and enzyme horseradish peroxidase (hrp), which attached to the aptamer via biotin-s ...	2014	25172129
development of an enzyme-linked immunospot assay for determination of rotavirus infectivity.	conventional rotavirus infectivity assays are time consuming, labor intensive, and with low sample throughput. to overcome these problems, a 96-well microplate enzyme-linked immunospot assay (elispot) was developed for the measurement of rotavirus infectious titers. the infected ma104 cells were stained with a horseradish peroxidase-conjugated anti-vp6 monoclonal antibody followed by detection with an elispot analyzer. a linear relationship was found between spot number and input of rotavirus do ...	2014	25172048
electron paramagnetic resonance and electron-nuclear double resonance studies of the reactions of cryogenerated hydroperoxoferric-hemoprotein intermediates.	the fleeting ferric peroxo and hydroperoxo intermediates of dioxygen activation by hemoproteins can be readily trapped and characterized during cryoradiolytic reduction of ferrous hemoprotein-o2 complexes at 77 k. previous cryoannealing studies suggested that the relaxation of cryogenerated hydroperoxoferric intermediates of myoglobin (mb), hemoglobin, and horseradish peroxidase (hrp), either trapped directly at 77 k or generated by cryoannealing of a trapped peroxo-ferric state, proceeds throug ...	2014	25046203
reversible chemical tuning of charge carriers for enhanced photoelectrochemical conversion and probing of living cells.	a facile, solution method for reversible tuning of oxygen vacancies inside tio2 nanowires, in which the reducing treatment of tio2 by nabh4 leads to 2.4-fold increase of photocurrent density, compared to pristine tio2 nanowires, is reported. subsequent oxidizing treatment using kmno4 or annealing in air can reset the photocurrent density to the original values. the incident photo-to-current conversion efficiency measurement exhibits that the reduced tio2 nanowires present both enhanced photoacti ...	2014	25044916
cell-free identification of novel n-myristoylated proteins from complementary dna resources using bioorthogonal myristic acid analogues.	to establish a non-radioactive, cell-free detection system for protein n-myristoylation, metabolic labeling in a cell-free protein synthesis system using bioorthogonal myristic acid analogues was performed. after cu(i)-catalyzed azide-alkyne cycloaddition (cuaac) with a biotin tag, the tagged proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and blotted on a polyvinylidene fluoride (pvdf) membrane, and then protein n-myristoylation was detected by en ...	2014	25043870
click synthesis of glucose-functionalized hydrophilic magnetic mesoporous nanoparticles for highly selective enrichment of glycopeptides and glycans.	selective enrichment of glycopeptides from complex biological samples is essential for ms-based glycoproteomics, but challenges still remain. in this work, glucose-functionalized magnetic mesoporous nanoparticles (mmns), which hold the attractive features of well-defined core/shell structure, high specific surface area (324m(2)/g), narrow pore size distribution (2.2nm) and high magnetic responsivity (69.1emug(-1)), were synthesized via click chemistry and applied to enrich glycopeptides and glyc ...	2014	25042437
effect of local application of transforming growth factor-β at the nerve repair site following chronic axotomy and denervation on the expression of regeneration-associated genes. laboratory investigation.	although peripheral nerves can regenerate after traumatic injury, functional recovery is often suboptimal, especially after injuries to large nerve trunks such as the sciatic nerve or brachial plexus. current research with animal models suggests that the lack of functional recovery resides in the lack of sufficient mature axons reaching their targets due to the loss of neurotrophic support by schwann cells in the distal stump of injured nerves. this study was designed to investigate the effect o ...	2014	25036208
naturally-occurring tetrahydro-β-carboline alkaloids derived from tryptophan are oxidized to bioactive β-carboline alkaloids by heme peroxidases.	β-carbolines are indole alkaloids that occur in plants, foods, and endogenously in mammals and humans, and which exhibit potent biological, psychopharmacological and toxicological activities. they form from naturally-occurring tetrahydro-β-carboline alkaloids arising from tryptophan by still unknown way and mechanism. results in this research show that heme peroxidases catalyzed the oxidation of tetrahydro-β-carbolines (i.e. 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and 1-methyl-1,2,3,4-t ...	2014	25035927
in vivo bioluminescence imaging for viable human neural stem cells incorporated within in situ gelatin hydrogels.	three-dimensional (3d) hydrogel-based stem cell therapies contribute to enhanced therapeutic efficacy in treating diseases, and determining the optimal mechanical strength of the hydrogel in vivo is important for therapeutic success. we evaluated the proliferation of human neural stem cells incorporated within in situ-forming hydrogels and compared the effect of hydrogels with different elastic properties in cell/hydrogel-xenografted mice.	2014	26116122
metabotropic glutamate receptor 5 shows different patterns of localization within the parallel visual pathways in macaque and squirrel monkeys.	glutamate is used as an excitatory neurotransmitter by the koniocellular (k), magnocellular (m), and parvocellular (p) pathways to transfer signals from the primate lateral geniculate nucleus (lgn) to primary visual cortex (v1). glutamate acts through both fast ionotropic receptors, which appear to carry the main sensory message, and slower, modulatory metabotropic receptors (mglurs). in this study, we asked whether mglur5 relates in distinct ways to the k, m, and p lgn axons in v1. to answer th ...	2014	25774086
dealing with large sample sizes: comparison of a new one spot dot blot method to western blot.	western blot is the gold standard method to determine individual protein expression levels. however, western blot is technically difficult to perform in large sample sizes because it is a time consuming and labor intensive process. dot blot is often used instead when dealing with large sample sizes, but the main disadvantage of the existing dot blot techniques, is the absence of signal normalization to a housekeeping protein.	2014	25648029
detection of the inflammation biomarker c-reactive protein in serum samples: towards an optimal biosensor formula.	the development of an electrochemical immunosensor for the biomarker, c-reactive protein (crp), is reported in this work. crp has been used to assess inflammation and is also used in a multi-biomarker system as a predictive biomarker for cardiovascular disease risk. a gold-based working electrode sensor was developed, and the types of electrode printing inks and ink curing techniques were then optimized. the electrodes with the best performance parameters were then employed for the construction ...	2014	25587427
a novel approach to investigate the effect of methionine oxidation on pharmacokinetic properties of therapeutic antibodies.	preserving the chemical and structural integrity of therapeutic antibodies during manufacturing and storage is a major challenge during pharmaceutical development. oxidation of fc methionines met252 and met428 is frequently observed, which leads to reduced affinity to fcrn and faster plasma clearance if present at high levels. because oxidation occurs in both positions simultaneously, their individual contribution to the concomitant changes in pharmacokinetic properties has not been clearly esta ...	2014	25517308
construction of a synthetic phage-displayed nanobody library with cdr3 regions randomized by trinucleotide cassettes for diagnostic applications.	nanobodies (nbs) have proved their great value as therapeutic molecules and clinical diagnostic tools. although the routine procedure to obtain nbs is to immunize camels with antigens, it is unavailable to immunize a camel when the antigens are highly toxic, pathogenic or nonimmunogenic. a synthetic phage display library is an alternative to generate nbs against such targets, besides all the other ones.	2014	25496223
autophagy modulates snca/α-synuclein release, thereby generating a hostile microenvironment.	snca/α-synuclein aggregation plays a crucial role in synucleinopathies such as parkinson disease and dementia with lewy bodies. aggregating and nonaggregating snca species are degraded by the autophagy-lysosomal pathway (alp). previously, we have shown that the alp is not only responsible for snca degradation but is also involved in the intracellular aggregation process of snca. an additional role of extracellular snca in the pathology of synucleinopathies substantiating a prion-like propagation ...	2014	25484190
generation of novel monoclonal antibodies for the enrichment and characterization of human corneal endothelial cells (hcenc) necessary for the treatment of corneal endothelial blindness.	corneal transplantation is the primary treatment option to restore vision for patients with corneal endothelial blindness. although the success rate of treatment is high, limited availability of transplant grade corneas is a major obstacle. tissue-engineered corneal endothelial grafts constructed using cultivated human corneal endothelial cells (hcenc) isolated from cadaveric corneas may serve as a potential graft source. currently, tools for the characterization of cultured hcenc and enrichment ...	2014	25484056
critical role of bioanalytical strategies in investigation of clinical pk observations, a phase i case study.	rg7652 is a human immunoglobulin 1 (igg1) monoclonal antibody (mab) targeting proprotein convertase subtilisin/kexin type 9 (pcsk9) and is designed for the treatment of hypercholesterolemia. a target-binding enzyme-linked immunosorbent assay (elisa) was developed to measure rg7652 levels in human serum in a phase i study. although target-binding assay formats are generally used to quantify free therapeutic, the actual therapeutic species being measured are affected by assay conditions, such as s ...	2014	25484037
activation volumes of enzymes adsorbed on silica particles.	the immobilization of enzymes on carrier particles is useful in many biotechnological processes. in this way, enzymes can be separated from the reaction solution by filtering and can be reused in several cycles. on the other hand, there is a series of examples of free enzymes in solution that can be activated by the application of pressure. thus, a potential loss of enzymatic activity upon immobilization on carrier particles might be compensated by pressure. in this study, we have determined the ...	2014	25479476
epithelial cell-derived periostin functions as a tumor suppressor in gastric cancer through stabilizing p53 and e-cadherin proteins via the rb/e2f1/p14arf/mdm2 signaling pathway.	periostin is usually considered as an oncogene in diverse human cancers, including breast, prostate, colon, esophagus, and pancreas cancers, whereas it acts as a tumor suppressor in bladder cancer. in gastric cancer, it has been demonstrated that periglandular periostin expression is decreased whereas stromal periostin expression is significantly increased as compared with normal gastric tissues. moreover, periostin produced by stromal myofibroblasts markedly promotes gastric cancer cell growth. ...	2014	25486483
a novel atg4b antagonist inhibits autophagy and has a negative impact on osteosarcoma tumors.	autophagy has been implicated in the progression and chemoresistance of various cancers. in this study, we have shown that osteosarcoma saos-2 cells lacking atg4b, a cysteine proteinase that activates lc3b, are defective in autophagy and fail to form tumors in mouse models. by combining in silico docking with in vitro and cell-based assays, we identified small compounds that suppressed starvation-induced protein degradation, lc3b lipidation, and formation of autophagic vacuoles. nsc185058 effect ...	2014	25483883
interfacing polymeric scaffolds with primary pancreatic ductal adenocarcinoma cells to develop 3d cancer models.	we analyzed the interactions between human primary cells from pancreatic ductal adenocarcinoma (pdac) and polymeric scaffolds to develop 3d cancer models useful for mimicking the biology of this tumor. three scaffold types based on two biocompatible polymeric formulations, such as poly(vinyl alcohol)/gelatin (pva/g) mixture and poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (peot/pbt) copolymer, were obtained via different techniques, namely, emulsion and freeze-drying, compress ...	2014	25482337
bactrian camel nanobody-based immunoassay for specific and sensitive detection of cry1fa toxin.	the variable domain of the heavy-chain-only antibody (vhh) or nanobody (nb), derived from camelids, begins to play an important role on the detection of protein markers. in this study, we constructed a phage-displayed library of vhhs against cry1fa by immunizing a healthy bactrian camel with cry1fa toxin. after a series of bio-panning and screening by phage display technology, three anti-cry1fa nanobodies (nbs) with great difference in complementarity determining region 3 (cdr3) were obtained an ...	2014	25448390
quantification of 5-methylcytosine, 5-hydroxymethylcytosine and 5-carboxylcytosine from the blood of cancer patients by an enzyme-based immunoassay.	genome-wide aberrations of the classic epigenetic modification 5-methylcytosine (5mc), considered the hallmark of gene silencing, has been implicated to play a pivotal role in mediating carcinogenic transformation of healthy cells. recently, three epigenetic marks derived from enzymatic oxidization of 5mc namely 5-hydroxymethylcytosine (5hmc), 5-formylcytosine (5fc) and 5-carboxylcytosine (5cac), have been discovered in the mammalian genome. growing evidence suggests that these novel bases posse ...	2014	25441900
histochemical study of retinal photoreceptors development during pre- and postnatal period and their association with retinal pigment epithelium.	the aim of this study was to evaluate distribution and changes of glycoconjugates of retinal photoreceptors during both pre- and postnatal development.	2014	25429338
oligomerization of optically active n-(4-hydroxyphenyl)mandelamide in the presence of β-cyclodextrin and the minor role of chirality.	the oxidative oligomerization of a chiral mandelamide derivative (n-(4-hydroxyphenyl)mandelamide, 1) was performed in the presence of horseradish peroxidase, laccase and n,n'-bis(salicylidene)ethylenediamine-iron(ii) to obtain chiral oligophenols 2. the low enantioselectivity of the enzymatic catalyzed asymmetric enantiomer-differentiating oligomerizations was investigated. in addition, the poor influence of cyclodextrin on the enantioselectivity of enzymatic catalyzed asymmetric enantiomer-diff ...	2014	25383106
two-stage nicking enzyme signal amplification combined with dnazyme amplification for the detection of bone morphogenetic protein 6 mrna.	highly sensitive detection of bone morphogenetic protein 6 (bmp6) mrna is essential to monitor bone regeneration in the regenerating defects. in this work, we proposed a quantitative approach based on two-stage nicking enzyme signal amplification (nesa) and dnazyme amplification for highly sensitive detection of bmp6 mrna. the two-stage nesa involves two templates and two-stage amplification reactions under isothermal conditions. the first template contains two repeat sequences that could hybrid ...	2014	25382038
highly tunable elastomeric silk biomaterials.	elastomeric, fully degradable and biocompatible biomaterials are rare, with current options presenting significant limitations in terms of ease of functionalization and tunable mechanical and degradation properties. we report a new method for covalently crosslinking tyrosine residues in silk proteins, via horseradish peroxidase and hydrogen peroxide, to generate highly elastic hydrogels with tunable properties. the tunable mechanical properties, gelation kinetics and swelling properties of these ...	2014	25395921
inhibition of cpla2 has neuroprotective effects on motoneuron and muscle atrophy following spinal cord injury.	surviving motoneurons undergo dendritic atrophy after spinal cord injury (sci), suggesting an important therapeutic target for neuroprotective strategies to improve recovery of function after sci. our previous studies showed that phospholipase a2 (pla2) may play an important role in the pathogenesis of sci. in the present study, we investigated whether blocking cpla2 pharmacologically with arachidonyl trifluoromethyl ketone (atk) or genetically using cpla2 knockout (ko) mice attenuates motoneuro ...	2014	25386720
craniocerebral injury promotes the repair of peripheral nerve injury.	the increase in neurotrophic factors after craniocerebral injury has been shown to promote fracture healing. moreover, neurotrophic factors play a key role in the regeneration and repair of peripheral nerve. however, whether craniocerebral injury alters the repair of peripheral nerve injuries remains poorly understood. rat injury models were established by transecting the left sciatic nerve and using a free-fall device to induce craniocerebral injury. compared with sciatic nerve injury alone aft ...	2014	25374593
identification of hydrogen peroxide-secreting cells by cytocompatible coating with a hydrogel membrane.	a method for identifying each cell secreting reactive oxygen species (ros) is highly desirable to advance the understanding of the physiological and pathological processes attributed to extracellular ros. here, we first report a method for realizing this. the individual cells secreting hydrogen peroxide (h2o2), a common ros, could be coated by a hydrogel membrane through a horseradish peroxidase-catalyzed reaction consuming h2o2 secreted from the cells themselves. this hydrogel membrane coating ...	2014	25359398
early maternal separation induces alterations of colonic epithelial permeability and morphology.	early maternal separation could lead to significant intestinal barrier and epithelial dysfunction. however, the exact mechanism remains to be elucidated and need to be investigated.	2014	25358892
development of monoclonal antibody-based sandwich elisa for detection of dextran.	dextran as anti-nutritional factor is usually a result of bacteria activity and has associated serial problems during the process stream in the sugar industry and in medical therapy. a sensitive method is expected to detect dextran quantitatively. here we generated four monoclonal antibodies (mabs) against dextran using dextran t40 conjugated with bovine serum albumin (bsa) as immunogen in our lab following hybridoma protocol. through pairwise, an mab named d24 was determined to be conjugated wi ...	2014	25358002
conserved dopamine neurotrophic factor-transduced mesenchymal stem cells promote axon regeneration and functional recovery of injured sciatic nerve.	peripheral nerve injury (pni) is a common disease that often results in axonal degeneration and the loss of neurons, ultimately leading to limited nerve regeneration and severe functional impairment. currently, there are no effective treatments for pni. in the present study, we transduced conserved dopamine neurotrophic factor (cdnf) into mesenchymal stem cells (mscs) in collagen tubes to investigate their regenerative effects on rat peripheral nerves in an in vivo transection model. scanning el ...	2014	25343619
a 3d-printed device for a smartphone-based chemiluminescence biosensor for lactate in oral fluid and sweat.	increasingly, smartphones are used as portable personal computers, revolutionizing communication styles and entire lifestyles. using 3d-printing technology we have made a disposable minicartridge that can be easily prototyped to turn any kind of smartphone or tablet into a portable luminometer to detect chemiluminescence derived from enzyme-coupled reactions. as proof-of-principle, lactate oxidase was coupled with horseradish peroxidase for lactate determination in oral fluid and sweat. lactate ...	2014	25343380
"ready-to-use" hollow nanofiber membrane-based glucose testing strips.	a novel "ready-to-use" glucose test strip based on a polyurethane hollow nanofiber membrane was fabricated through facile co-axial electrospinning. by utilizing glucose oxidase and horseradish peroxidase in the core-phase solution, and a chromogenic agent either in the core solution (in which case 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (abts) was used) or in the shell-phase solution (in which case o-dianisidine was used) for co-axial electrospinning, in situ co-encapsulation of the t ...	2014	25343161
metal-organic framework-derived copper nanoparticle@carbon nanocomposites as peroxidase mimics for colorimetric sensing of ascorbic acid.	metal-organic frameworks (mofs) have emerged as very fascinating functional materials due to their diversity nature. a nanocomposite consisting of copper nanoparticles dispersed within a carbon matrix (cu nps@c) is prepared through a one-pot thermolysis of copper-based metal-organic framework precursors. cu nps@c can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (tmb) to form a colored product in the presence of h2 o2 . as a peroxidase mimic, cu nps@c not only has the advantages of lo ...	2014	25332148
overelaborated synaptic architecture and reduced synaptomatrix glycosylation in a drosophila classic galactosemia disease model.	classic galactosemia (cg) is an autosomal recessive disorder resulting from loss of galactose-1-phosphate uridyltransferase (galt), which catalyzes conversion of galactose-1-phosphate and uridine diphosphate (udp)-glucose to glucose-1-phosphate and udp-galactose, immediately upstream of udp-n-acetylgalactosamine and udp-n-acetylglucosamine synthesis. these four udp-sugars are essential donors for driving the synthesis of glycoproteins and glycolipids, which heavily decorate cell surfaces and ext ...	2014	25326312
glycine oxidase based high-throughput solid-phase assay for substrate profiling and directed evolution of (r)- and (s)-selective amine transaminases.	transaminases represent one of the most important enzymes of the biocatalytic toolbox for chiral amine synthesis as they allow asymmetric synthesis with quantitative yields and high enantioselectivity. in order to enable substrate profiling of transaminases for acceptance of different amines, a glycine oxidase and horseradish peroxidase coupled assay was developed. transaminase activity is detected upon transfer of an amine group from an amino donor substrate to glyoxylate, generating glycine, w ...	2014	25321325
tio2@ceox core-shell nanoparticles as artificial enzymes with peroxidase-like activity.	the ce4+↔ce3+ redox switch is at the basis of an all-inorganic catalytic cycle that is capable of mimicking the activity of several natural redox enzymes. the efficiency of these artificial enzymes (nanozymes) strongly depends on the ce4+/ce3+ ratio. by capitalizing on the results obtained on oxide/oxide model systems, we implemented a simple and effective procedure to obtain conformal tio2@ceox core-shell nanoparticles whose thickness is controlled with single-layer precision. since the ce3+ sp ...	2014	25321080
polymerization amplified detection for nanoparticle-based biosensing.	efficient signal amplification processes are key to the design of sensitive assays for biomolecule detection. here, we describe a new assay platform that takes advantage of both polymerization reactions and the aggregation of nanoparticles to amplify signal. in our design, a cascade is set up in which radicals generated by either enzymes or metal ions are polymerized to form polymers that can entangle multiple gold nanoparticles (aunps) into aggregates, resulting in a visible color change. less ...	2014	25315059
how modification of accessible lysines to phenylalanine modulates the structural and functional properties of horseradish peroxidase: a simulation study.	horseradish peroxidase (hrp) is one of the most studied peroxidases and a great number of chemical modifications and genetic manipulations have been carried out on its surface accessible residues to improve its stability and catalytic efficiency necessary for biotechnological applications. most of the stabilized derivatives of hrp reported to date have involved chemical or genetic modifications of three surface-exposed lysines (k174, k232 and k241). in this computational study, we altered these ...	2014	25313804
simultaneous detection of forbidden chemical residues in milk using dual-label time-resolved reverse competitive chemiluminescent immunoassay based on amine group functionalized surface.	in this study, a sensitive dual-label time-resolved reverse competitive chemiluminescent immunoassay was developed for simultaneous detection of chloramphenicol (cap) and clenbuterol (cle) in milk. the strategy was performed based on the distinction of the kinetic characteristics of horseradish peroxidase (hrp) and alkaline phosphatase (alp) in chemiluminesecence (cl) systems and different orders of magnitude in hrp cl value for cap and alp cl value for cle in the chemiluminescent immunoassay. c ...	2014	25313517
spatial distance effect of bienzymes on the efficiency of sequential reactions in a microfluidic reactor packed with enzyme-immobilized microbeads.	three different configurations of microfluidic reactors packed with enzyme-bearing microbeads were examined to show that the overall efficiency of coupled enzyme-catalyzed reactions depends on the spatial relationship of two enzymes immobilized on the bead surfaces. the spatial distances of glucose oxidase (gox) and horseradish peroxidase (hrp) enzymes were controlled by using microbeads as a supporting matrix for immobilizing the two enzymes and packing them in two microfluidic chambers. a micr ...	2014	25312630
switchable enzyme/dnazyme cascades by the reconfiguration of dna nanostructures.	mimicking cellular transformations and signal transduction pathways by means of biocatalytic cascades proceeding in organized media is a scientific challenge. we describe two dna machines that enable the "on/off" switchable activation and deactivation of three-component biocatalytic cascades. one system consists of a reconfigurable dna tweezers-type structure, whereas in the second system the catalytic cascade proceeds on a switchable dna clamp scaffold. the three-component catalytic cascades co ...	2014	25308317
sulforaphane homologues: enantiodivergent synthesis of both enantiomers, activation of the nrf2 transcription factor and selective cytotoxic activity.	reported is an enantiodivergent approach for the synthesis of both enantiomers of sulforaphane (sfn) homologues with different chain lengths between the sulfinyl sulfur and the isothiocyanate groups and different substituents on the sulfinyl sulfur. the homologues were designed in order to unravel the effect of all the diversity elements included in sulforaphane's structure. the key step of the approach is the diastereoselective synthesis of both sulfinate ester epimers at sulfur, using as singl ...	2014	25299679
hemin-block copolymer micelle as an artificial peroxidase and its applications in chromogenic detection and biocatalysis.	following an inspiration from the fine structure of natural peroxidases, such as horseradish peroxidase (hrp), an artificial peroxidase was constructed through the self-assembly of diblock copolymers and hemin, which formed a functional micelle with peroxidase-like activity. the pyridine moiety in block copolymer poly(ethylene glycol)-block-poly(4-vinylpyridine) (peg-b-p4vp) can coordinate with hemin, and thus hemin is present in a five-coordinate complex with an open site for binding substrates ...	2014	25286878
conformational evaluation of hiv-1 trimeric envelope glycoproteins using a cell-based elisa assay.	hiv-1 envelope glycoproteins (env) mediate viral entry into target cells and are essential to the infectious cycle. understanding how those glycoproteins are able to fuel the fusion process through their conformational changes could lead to the design of better, more effective immunogens for vaccine strategies. here we describe a cell-based elisa assay that allows studying the recognition of trimeric hiv-1 env by monoclonal antibodies. following expression of hiv-1 trimeric env at the surface of ...	2014	25286159
[the establishment of a magnetic nanoparticle-based immunocapturing method for the detection of abrin].	to develop a high sensitive and specific method for the detection of abrin.	2014	25270215
small-molecule triggered cascade enzymatic catalysis in hour-glass shaped nanochannel reactor for glucose monitoring.	construction of an electrical signal-sensitive nanoreactor in response to small molecule remains a challenge in the developing fields of biomimetic device. solid nanochannels are considered as promising candidates for constructing smart systems, which is highly sensitive to biochemical stimulus. here, we report an hourglass shaped nanochannel reactor based on cascade enzymatic catalysis and cation-selective nanochannel system. the employed glucose-specific dual-enzyme combination, glucose oxidas ...	2014	25268828
immobilization of horseradish peroxidase on nh2-modified magnetic fe3o4/sio2 particles and its application in removal of 2,4-dichlorophenol.	fe3o4 nanoparticles were prepared by a co-precipitation method with the assistance of ultrasound irradiation, and then coated with silica generated by hydrolysis and condensation of tetraethoxysilane. the silica-coated fe3o4 nanoparticles were further modified with 3-aminopropyltriethoxysilane, resulting in anchoring of primary amine groups on the surface of the particles. horseradish peroxidase (hrp) was then immobilized on the magnetic core-shell particles by using glutaraldehyde as a crosslin ...	2014	25268726
fast and automatic imaging of immunoenzyme-stained neuronal circuits in the whole brain of drosophila.	knowledge of neuronal wiring and morphogenesis in drosophila is essential to understand brain function and dysfunction. the immunoenzyme method based on horseradish peroxidase/diaminobenzidine (hrp/dab) provides high-contrast images to resolve details underlying neuronal architecture. however, the poor staining penetration and a lack of corresponding three-dimensional imaging methodology limit its application. herein, we modified the hrp/dab method to stain neuronal circuits in the whole brain o ...	2014	25255400
evaluation of textile dye degradation due to the combined action of enzyme horseradish peroxidase and hydrogen peroxide.	the kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (hrp) with hydrogen peroxide in the degradation of methylene blue dye were investigated. twenty-one percent of color removal was obtained at ph 5.0 and temperature of 30 °c. under these conditions, the kinetic parameters k m and v max of enzymatic reactions were determined for hydrogen peroxide in the absence of methylene blue dye (k m = 17.3 mm; v max = 1.97 mm/min) and in the presence of methylene ...	2014	25248990
in situ formation of enzyme-free hydrogels via ferromagnetic microbead-assisted enzymatic cross-linking.	in situ formation of horseradish peroxidase (hrp)-free gelatin hydrogels was achieved via ferromagnetic microbead-assisted enzymatic cross-linking. gelation time and mechanical stiffness of the hydrogels can be tuned in situ, which makes hrp-free gelatin hydrogels suitable for injectable cell delivery.	2014	25247682
rapid and simultaneous detection of ricin, staphylococcal enterotoxin b and saxitoxin by chemiluminescence-based microarray immunoassay.	simultaneous detection of small and large molecules on microarray immunoassays is a challenge that limits some applications in multiplex analysis. this is the case for biosecurity, where fast, cheap and reliable simultaneous detection of proteotoxins and small toxins is needed. two highly relevant proteotoxins, ricin (60 kda) and bacterial toxin staphylococcal enterotoxin b (seb, 30 kda) and the small phycotoxin saxitoxin (stx, 0.3 kda) are potential biological warfare agents and require an anal ...	2014	25237676
pathological changes in the maxillary sinus mucosae of patients with recurrent odontogenic maxillary sinusitis.	to study the structural and functional changes of maxillary sinus mucosae of patients with odontogenic maxillary sinusitis, and to improve the therapeutic effects.	2014	25225509
immunohistochemical assessment of proliferating cell nuclear antigen protein expression in plaque, reticular and erosive types of oral lichen planus.	proliferating cell nuclear antigen (pcna) is a nuclear protein synthesized in the late g1 and s-phase of the cell cycle. immunodetection of this protein represents a useful marker of the proliferation status of lesions.	2014	25221712
combined experimental and theoretical study on the reactivity of compounds i and ii in horseradish peroxidase biomimetics.	for the exploration of the intrinsic reactivity of two key active species in the catalytic cycle of horseradish peroxidase (hrp), compound i (hrp-i) and compound ii (hrp-ii), we generated in situ [fe(iv) o(tmp(+.) )(2-meim)](+) and [fe(iv) o(tmp)(2-meim)](0) (tmp=5,10,15,20-tetramesitylporphyrin; 2-meim=2-methylimidazole) as biomimetics for hrp-i and hrp-ii, respectively. their catalytic activities in epoxidation, hydrogen abstraction, and heteroatom oxidation reactions were studied in acetoni ...	2014	25220399
enzymatic degradation of oxidized and reduced graphene nanoribbons by lignin peroxidase.	the expanding use of graphene for various industrial and biomedical applications requires efficient remediation strategies during their disposal into waste streams. additionally, the interactions of graphene with the biota need thorough evaluation. in this study, we investigated the interactions of oxidized and reduced graphene oxide nanoribbons (gonrs and rgonrs) with lignin peroxidase (lip), a ligninolytic enzyme released from white rot fungus. gonrs and rgonrs were treated with lip in the pre ...	2014	25215188