Publications

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cxcl1 contributes to β-amyloid-induced transendothelial migration of monocytes in alzheimer's disease.bone marrow-derived microglia that originates in part from hematopoietic cells, and more particularly from monocytes preferentially attach to amyloid deposition in brains of alzheimer's disease (ad). however, the mechanism of monocytes recruited into the amyloid plaques with an accelerated process in ad is unclear.201323967336
probing horseradish peroxidase catalyzed degradation of azo dye from tannery wastewater.biocatalysis based effluent treatment has outclassed the presently favored physico-chemical treatments due to nil sludge production and monetary savings. azo dyes are commonly employed in the leather industry and pose a great threat to the environment. here, we show the degradation of c. i. acid blue 113 using horseradish peroxidase (hrp) assisted with h2o2 as a co-substrate. it was observed that 0.08 u hrp can degrade 3 ml of 30 mg/l dye up to 80% within 45 min with the assistance of 14 μl of h ...201323961406
superparamagnetic beads for estimation of spinal subarachnoid space permeability in rats.human spinal pathological processes have been linked to a loss of spinal subarachnoid space (ssas) permeability, which has therefore become a target for therapy. hence, it has become important to measure ssas patency in rat models of these human disorders.201323958748
organic cation transporter 2 (slc22a2), a low-affinity and high-capacity choline transporter, is preferentially enriched on synaptic vesicles in cholinergic neurons.organic cation transporters (octs) are expressed mainly in the kidney and liver. octs transport intrinsic organic cations, including monoamine, dopamine, serotonine and choline, across the plasma membrane. here, we demonstrate that oct2 (slc22a2) is expressed in cholinergic neurons, motoneurons in the anterior horn of the spinal cord, and is implicated in acetylcholine (ach) recycling in presynaptic terminals. application of rabbit anti-peptide antibody revealed that oct2 was expressed in the an ...201323958595
the influence of angiotensin-(1-7) mas receptor agonist (ave 0991) on mitochondrial proteome in kidneys of apoe knockout mice.excessive action of angiotensin ii on mitochondria has been shown to play an important role in mitochondrial dysfunction, a common feature of atherogenesis and kidney injury. angiotensin-(1-7)/mas receptor axis constitutes a countermeasure to the detrimental effects of angiotensin ii on at1 receptors. the aim of the study was to assess the effects of angiotensin-(1-7) peptidomimetic ave0991 on the kidney mitochondrial proteome in widely used animal model of atherosclerosis (apoe(-/-) mice). prot ...201323988828
spinal interleukin-33 and its receptor st2 contribute to bone cancer-induced pain in mice.cancer pain, particularly bone cancer pain, affects the quality of life of cancer patients, and current treatments are limited. interleukin (il)-33, a new member of the il-1 super family, has been reported to be involved in the modulation of inflammatory pain. however, studies focused on its role in the modulation of cancer pain have been rare. the present study was designed to investigate whether spinal il-33/st2 signaling was involved in bone cancer-induced pain in mice. bone cancer was induce ...201323988433
kinetic rotating droplet electrochemistry: a simple and versatile method for reaction progress kinetic analysis in microliter volumes.here, we demonstrate a new generic, affordable, simple, versatile, sensitive, and easy-to-implement electrochemical kinetic method for monitoring, in real time, the progress of a chemical or biological reaction in a microdrop of a few tens of microliters, with a kinetic time resolution of ca. 1 s. the methodology is based on a fast injection and mixing of a reactant solution (1-10 μl) in a reaction droplet (15-50 μl) rapidly rotated over the surface of a nonmoving working electrode and on the re ...201323985016
introducing novel amorphous carbon nanoparticles as energy acceptors into a chemiluminescence resonance energy transfer immunoassay system.a novel chemiluminescence resonance energy transfer (cret) system for competitive immunoassay of biomolecules was developed by using novel amorphous carbon nanoparticles (cnps) prepared from candle soot as energy acceptors. the cnps were firstly prepared to bind with the antigen (ag) for obtaining the nanocomposite cnp-ag, and this obtained cnp-ag was then reacted with the horseradish peroxidase-labeled antibody (hrp-ab) to assemble the cret system. the luminol catalyzed by hrp serving as the en ...201323979821
[establishment of the dot immunoenzyme filtration assay for quantitative detection of estriol].to make anti-estriol (e3) monoclonal antibody (mab) with high specificity, and develop a rapid, simple and sensitive method for the detection of e3 in county-level institutions.201323948419
direct replacement of antibodies with molecularly imprinted polymer nanoparticles in elisa--development of a novel assay for vancomycin.a simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanomips) to develop assays similar to the enzyme-linked immunosorbent assay (elisa) is presented here for the first time. nanomips were synthesized by a solid-phase approach with an immobilized vancomycin (template) and characterized using biacore 3000, dynamic light scattering, and electron microscopy. immobilization, blocking, and washing conditions were optimized in microplat ...201323947402
n-myc knockdown and apigenin treatment controlled growth of malignant neuroblastoma cells having n-myc amplification.malignant neuroblastomas mostly occur in children and are frequently associated with n-myc amplification. oncogene amplification, which is selective increase in copy number of the oncogene, provides survival advantages in solid tumors including malignant neuroblastoma. we have decreased expression of n-myc oncogene using short hairpin rna (shrna) plasmid to increase anti-tumor efficacy of the isoflavonoid apigenin (apg) in human malignant neuroblastoma sk-n-dz and sk-n-be2 cell lines that harbor ...201323941992
flow system for the automatic screening of the effect of phenolic compounds on the luminol-hydrogen peroxide-peroxidase chemiluminescence system.in this work, an automated flow-based procedure for the screening of the effect of the different phenolic compounds on the chemiluminescence (cl) luminol-hydrogen peroxide-horseradish peroxidase (hrp) system is presented. this procedure involves the combination of multisyringe flow injection analysis (mfsia) and sequential injection analysis (sia) techniques and exploits the ability of the different subgroups of phenols, such as cholorophenols, nitrophenols, methylphenols and polyphenols, to enh ...201321308949
peroxidase-mediated polymerization of 1-naphthol: impact of solution ph and ionic strength.peroxidase-mediated oxidation has been proposed as a treatment method for naphthol-contaminated water. however, the impact of solution chemistry on naphthol polymerization and removal has not been documented. this research investigated the impact of ph and ionic strength on peroxidase-mediated removal of 1-naphthol in completely mixed batch reactors. the impact of hydrogen peroxide to 1-naphthol ratio and activity of horseradish peroxidase was also studied. size exclusion chromatography was used ...201319704146
[catalytic activity and the stability of horseradish peroxidase increase as a result of its incorporation into a polyelectrolyte complex with chitosan].the incorporation of horseradish peroxidase into polyelectrolyte complexes with chitosans of different molecular weights (mw 5-150 kda) yielded highly active and stable enzyme preparations. as a result of the selection of optimal conditions for the formation of peroxidase-chitosan complexes, it was found that 0.1% chitosan with a mw of 10 kda had the strongest activatory effect on peroxidase (activation degree, > 70%) in the reaction of o-dianisidine oxidation by hydrogen peroxide. the complex f ...201319382699
automated determination of asulam by enhanced chemiluminescence using luminol/peroxidase system.a flow injection system with chemiluminescence detection for the determination of asulam, enhancer of the system luminol-h(2)o(2)-horseradish peroxidase, is proposed. the method shows a moderate selectivity against other pesticides usually present in formulations of herbicides and in water. the procedure was applied to the determination of asulam in tap water samples and a recovery study was carried out in order to validate the method. the obtained results show acceptable recovery values (betwee ...201319544320
[luminol oxidation by hydrogen peroxide with chemiluminescent signal formation catalyzed by peroxygenase from the fungus agrocybe aegerita v.brig].conditions of luminol oxidation by hydrogen peroxide in the presence of peroxygenase from the mushroom agrocybe aegerita v.brig have been optimized. the ph value (8.8) at which fungal peroxygenase produces a maximum chemiluminescent signal has been shown to be similar to the ph optimum value of horseradish peroxidase. luminescence intensity changed when the concentration of tris buffer was varied; maximum intensity of chemiluminescence was observed in 40 mm solution. it has been shown that enhan ...201320198921
trace-level detection of atrazine using immuno-chemiluminescence: dipstick and automated flow injection analyses formats.a sensitive chemiluminescence (cl)-based immunoassay technique based on both dipstick and flow injection analytical formats is reported for the detection of atrazine. in the dipstick-based immunoassay technique, antibody (anti-atrazine) was first immobilized on the nitrocellulose membranes. the dipstick was then treated with atrazine and atrazine-horseradish peroxidase conjugate (atra-hrp) to facilitate the competitive binding. the dipstick was further treated with urea-hydrogen peroxide (u-h202 ...201320334163
biological and enzymatic treatment of bisphenol a and other endocrine disrupting compounds: a review.bisphenol a is predominantly used as an intermediate in the production of polycarbonate plastics and epoxy resins. traces of bisphenol a released into the environment can reach into the wastewater and soil via application of sewage sludge from wastewater treatment systems that receive water containing bisphenol a, or from leachate from uncontrolled landfills. in this study we have made an effort to review the work on the presence of bisphenol a and other related endocrine disrupting compounds in ...201322712546
electrochemical immunoassay on expression of integrin β1 on tumor cells and drug-resistant tumor cells.an electrochemical indirect competitive immunoassay protocol as a promising cytosensing strategy was developed to detect integrin β1 expression on human breast cancer mcf-7 cells and adriamycin-resistant human breast cancer mcf-7 (mcf-7/adr) cells and quantify the cell number. integrin α5β1 was adsorbed on the gold-nanoparticle modified glassy carbon electrode to bind integrin β1 monoclonal antibody (anti-cd29 mab). a sandwich structure was then formed using nanocomposites which consisted of hor ...201322776180
a novel membrane-based process to isolate peroxidase from horseradish roots: optimization of operating parameters.the optimization of operating parameters for the isolation of peroxidase from horseradish (armoracia rusticana) roots with ultrafiltration (uf) technology was systemically studied. the effects of uf operating conditions on the transmission of proteins were quantified using the parameter scanning uf. these conditions included solution ph, ionic strength, stirring speed and permeate flux. under optimized conditions, the purity of horseradish peroxidase (hrp) obtained was greater than 84 % after a ...201322773175
chemiluminescence flow biosensor for glucose using mg-al carbonate layered double hydroxides as catalysts and buffer solutions.in this work, serving as supports in immobilizing luminol reagent, catalysts of luminol chemiluminescence (cl), and buffer solutions for the cl reaction, mg-al-co(3) layered double hydroxides (ldhs) were found to trigger luminol cl in weak acid solutions (ph 5.8). the silica sol-gel with glucose oxidase and horseradish peroxidase was immobilized in the first half of the inside surface of a clear quartz tube, and luminol-hybrid mg-al-co(3) ldhs were packed in the second half. therefore, a novel c ...201322770831
mgfe-layered double hydroxide modified electrodes for direct electron transfer of heme proteins.in this study, the fe-based layered double hydroxides (mg(3)fe ldh) were used to immobilize heme proteins including hemoglobin (hb), myoglobin (mb) and horseradish peroxidase (hrp) for fabrication of heme/mg(3)fe ldh film on glassy carbon electrode (mg(3)fe-heme/gce). the possible role of iron in framework of ldh to promote direct electron transfer (det) of heme proteins was investigated using an ldh containing non-iron as a reference. hb was selected as a model protein for studying the electroc ...201322721646
capillary electrophoresis-based immunoassay for the determination of brevetoxin-b in shellfish using electrochemical detection.neurotoxic shellfish poisoning is a significant food-borne disease caused by potent cyclic polyether toxins (brevetoxins) that accumulate in the flesh of shellfish. here we report a new procedure for brevetoxin analysis by capillary electrophoresis-based immunoassay (ce-ia) with electrochemical (ec) detection. in this method, after the competitive immunoreaction in liquid phase, the horseradish peroxidase (hrp)-labeled antigen and the bound enzyme-labeled complex were separated and then the syst ...201322781183
sulfate determines the glucosinolate concentration of horseradish in vitro plants (armoracia rusticana gaertn., mey. & scherb.).horseradish plants (armoracia rusticana) contain high concentrations of glucosinolates. former studies have revealed that armoracia plants cultivated in vitro have markedly lower glucosinolate concentrations than those grown in soils. yet, these studies neglected that the sulfate concentration in the growth medium may have had a strong impact on glucosinolate metabolism. accordingly, in this study horseradish in vitro plants were cultivated with differing sulfate concentrations and the glucosino ...201322886455
highly amplified electrochemiluminescence of peroxydisulfate using bienzyme functionalized palladium nanoparticles as labels for ultrasensitive immunoassay.an immunosensor based on the electrochemiluminescence (ecl) of peroxydisulfate was firstly proposed by coupling the cooperation of two enzymes to in situ generate coreactant with palladium nanoparticles (pdnps) as catalyst for the ecl reaction. pdnps were previously synthesized, which successfully attached to functional carbon nanotubes (fcnts), to bind the secondary antibody and bienzyme (horseradish peroxidase and glucose oxidase). then the prepared bioconjugates were introduced to the electro ...201322857905
enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes.the use of gold nanoparticles (aunps) as labeling carriers in combination with the enzymatic activity of the horseradish peroxidase (hrp) in order to achieve an improved optical lateral flow immunoassay (lfia) performance is presented here. briefly in a lfia with an immune-sandwich format aunps are functionalized with a detection antibody already modified with hrp, obtaining an 'enhanced' label. two different detection strategies have been tested: the first one following just the red color of th ...201322795532
adipose tissue engineering using adipose-derived stem cells enclosed within an injectable carboxymethylcellulose-based hydrogel.in situ gelation of an aqueous solution of carboxymethylcellulose derivative bearing phenolic hydroxyl groups (cmc-ph) that contained suspended adipose-derived stem cells (ascs) was studied in vitro and in vivo for evaluating feasibility in adipose tissue-engineering strategies. the rat ascs that were enclosed in the cmc-ph gels through a horseradish peroxidase-catalysed reaction showed 92.8% viability, good proliferation and adipogenic differentiation in vitro. ten weeks after the subcutaneous ...201322489051
development of an enzyme-linked immunoassay for the quantitation of influenza haemagglutinin: an alternative method to single radial immunodiffusion.the current method used to measure haemagglutinin (ha) content for influenza vaccine formulation, single radial immunodiffusion (srid), is lengthy and relies on the availability of matched standardised homologous reagents. the 2009 influenza pandemic highlighted the need to develop alternate assays that are able to rapidly quantitate ha antigen for vaccine formulation.201322583601
opposing effects of diabetes and tetracycline on the degradation of collagen membranes in rats.increased collagenolytic activity, characteristic of uncontrolled diabetes, may compromise collagen membrane (cm) survival. tetracycline (tcn) possesses anticollagenolytic properties and delays cm degradation in healthy animals. this study evaluates the degradation of tcn--immersed and -non-immersed cms in rats with diabetes compared to those with normoglycemia.201322631880
[a new look at reactions with participation of metal-porphyrines].reactions ofnucleophilic substitution and enzymatic processes with participation of metal-porphyrins (mp) are considered from the point of view of zn-tetraphenylporphin (zn-tphp) coordination with corresponding ligand/nucleophyl/substrate/base. linear correlations perform between kinetic parameters of process of coordination of zn-tphp in chloroform (constants of stability) and reactions of nucleophilic substitution both in aqueous and organic solvents with participation ofpyridines, n-oxides of ...201322792729
intact fibroblast growth factor 23 and fragments in plasma from gambian children.fibroblast growth factor 23 (fgf23) is grossly elevated in gambian children with rickets and, at a lower prevalence, in those without bone deformities. we used western blotting to mimic the detection capabilities of the c-terminal fgf23 enzyme-linked immunosorbent assay (elisa). only intact fgf23 hormone was present in gambian plasma samples from children with and without rickets.201322648001
anionic peroxidase production by arnebia euchroma callus.arnebia euchroma callus, obtained from the root cell culture of an iranian native specimen, has gained a doubling time of 63 h after regular subculturing on linsmaier-skoog (ls) medium containing sugar (50 g/l), 2,4-dichlorophenoxyacetic acid (10(-6) m), and kinetin (10(-5) m) under darkness at 25°c. despite the observed somaclonal variations, peroxidase production by the a. euchroma calli has been stable over 4 years under the aforementioned conditions. isoelectric focusing experiments revealed ...201322172108
development of raphe-spinal connections in the north american opossum.the falck-hillarp technique, serotonin (5-ht) immunohistochemistry and the retrograde transport of horseradish peroxidase (hrp) were utilized to investigate the development of raphe-spinal connections in the pouch-young opossum. the brainstem raphe and adjacent reticular formation contain 5-ht immunoreactive neurons in the newborn opossum (12 days after conception) and processes from these cells can be visualized in the marginal zone of the spinal cord. between eight and 15 days after birth 5-ht ...20136756557
[kinetics of peroxidase recombination with carbon dioxide in the +20 to -70 degree c temperature interval].using flash photolysis, horseradish peroxidase rebinding of carbon monoxide has been observed in the temperature range between 20 and -70 degrees c. a lag-period of about 50 ms immediately after photodissociation takes place. this lag-period is assumed to be due to the formation of the intermediate protein form with the low reactivity. following rebinding is bimolecular process with the rate constant 4 x 10(3) m-1 s-1 usually observed for the peroxidase reaction with carbon monoxide. for some pe ...20136797479
the morphologic effects of histamine on the lateral cochlear wall.the chinchilla lateral cochlear wall (stria vascularis, spiral ligament, and spiral prominence) was examined by morphologic and histochemical techniques following various doses of intravenous histamine. the three main findings were as follows: (1) the basic ultrastructure was not altered by histamine; (2) there is a time- and dose-dependent change in the rate of stria vascularis vessel permeability to a small protein tracer (horseradish peroxidase), but the mode of transport (large pore system) ...2013503533
[kinetics and mechanism of action of horseradish peroxidase in the reaction of dioxyfumaric acid oxidation with atmospheric oxygen].quantitative kinetic data are given on the oxidation reaction of dioxyfumaric acid (dfa) with atmospheric oxygen in the presence of horseradish peroxidase (hrp) depending on ph. activation constants of oxidation with hydrogen peroxide and mn ions are determined at ph 3.0. autocatalytic character of fra oxidation is shown to be due to the formation of h2o2 and other hydro peroxide-type compounds in the reaction, hrp convertions in the dfa--o2 system are studied using spectrophotometry. a mechanis ...20131108
endocytosis and phosphate transport in ok epithelial cells.endocytosis was studied in ok epithelial cells, an established cell line from opossum kidney. the presence of fluid-phase endocytosis in these cells was demonstrated by measuring cell uptake of lucifer yellow and horseradish peroxidase. the intracellular distribution of lucifer yellow fluorescence was consistent with uptake by endocytosis. endocytosis was inhibited in medium made hyperosmolar by addition of sucrose. in hyperosmolar medium the action of parathyroid hormone on na+/phosphate cotran ...20132623350
the effect of quenchers on the chemiluminescence of luminol and lucigenin.the chemiluminescence of luminol and lucigenin is often used to detect the production of reactive oxygen derivatives by phagocytic cells. also, several quenchers and enzyme inhibitors are used to determine which oxygen derivatives are responsible for the observed effects. in the present work we have assessed the reliability of dimethylthiourea and cysteamine (oh. quenchers), desferrioxamine (iron chelator) and diethyldithiocarbamate (superoxide dismutase inhibitor). they all react with cio- and ...20132728913
model systems for metabolism studies. biomimetic oxidation of acetaminophen and ellipticine derivatives with water-soluble metalloporphyrins associated to potassium monopersulfate.some original water-soluble metalloporphyrins/khso5 systems were developed to mimic the metabolic biooxidation of drugs. oxidation of acetaminophen and various ellipticine derivatives were used as model reactions. oxidative products (mainly quinone-imine structures) were obtained in good yield after 2 min of reaction, for a catalyst/substrate ratio of 0.04. iron(iii) derivative of tetrasodium meso-tetrakis(p-sulfonatophenyl)porphyrin and manganese(iii) derivative of tetraacetate meso-tetrakis(4- ...20131676637
[extraction of horseradish peroxidase and its conjugates formed by surface-active agent micelles].the author studied peculiarities of the extraction of horseradish peroxidase (hrp) and its conjugates with 3 and 7 molecules of progesterone (prog) from the aqueous solution into heptane and chloroform containing reversed micelles of surfactants. micelles of cetyltrimethylammonium bromide, aerosol ot, and triton x-45 protect the enzyme from denaturation in the biphasic system. the enzyme is readily extracted from the aqueous phase in the organic medium containing reversed micelles of surfactants ...20132082348
enzyme immunoassay for detection of drosophila melanogaster antigens in the juice of various foods.an enzyme immunoassay (eia) was developed to identify and quantitate soluble antigens originating from drosophila melanogaster eggs. polystyrene microtiter plates were coated with anti-egg antibody. egg antigen standards or samples were reacted with the sensitized wells. the immobilized antigen was reacted with biotinylated antibody, and the complex was quantitated by an avidin-horseradish peroxidase conjugate. the assay can detect egg antigens equivalent to 0.03 eggs/ml. the antibody was direct ...20133134333
[corticosteroids and cerebral vessel permeability during embryonic development].the effects of cortisol on the development of the blood-brain barrier (b.b.b.) were microscopically investigated in the chick embryo optic tectum using horseradish peroxidase (hrp) as marker of vascular permeability. hrp was injected intracardially at the 15th and 21st incubation day (i.d.), i.e. 5 and 11 days after the last administration of the drug (10 micrograms/50 microliters saline solution at the 8th and 10th i.d.). this treatment caused damage to the maturation process of the b.b.b. to h ...20132739535
sensitivity limitations encountered in enhanced horseradish peroxidase catalysed chemiluminescence.conditions for the enhanced horseradish peroxidase (hrp) catalysed reaction between luminol and hydrogen peroxide were optimized to determine detection limits for hrp conjugated to antibody fragment (hrp-fab) in solution phase. light output was linear with respect to hrp-fab concentration but became nonlinear at low hrp-fab concentrations when an accelerator (enhancer) of the reaction was used. para-phenylphenol was a more effective enhancer than p-iodophenol at hrp-fab concentrations below 20 p ...20133213591
enhanced chemiluminescence in the peroxidase-luminol-h2o2 system: anomalous reactivity of enhancer phenols with enzyme intermediates.phenols which markedly enhance chemiluminescence in the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide show anomalously high reactivity (by factors of approximately 10(2) compared with published hammett correlations) in the reduction of the enzyme intermediates, compound i and compound ii. the results support the hypothesis that efficient production of phenoxy radicals from phenols is a necessary criterion for chemiluminescence enhancer action.20132711890
cochlear vessel permeability to horseradish peroxidase after diuretic administration in the chinchilla.the permeability of strial vessels to the small protein, hrp, was examined after administration of diuretics to determine if increased vascular permeability is a factor in the development of strial edema as it is in acoustic trauma. chinchillas were injected with hrp and either ethacrynic acid, a loop-inhibiting diuretic, or mannitol, an osmotic diuretic. there was no increased vascular permeability to hrp. therefore, unlike the increased vessel permeability of hrp seen after an acoustic insult, ...20132511735
[properties of the halide complexes of horseradish peroxidase in the presence of aromatic substrates].the fluoride, chloride and bromide complexes of horseradish peroxidase were investigated by optical methods, nmr and esr. the galide binding may be increased or reduced by means of enzyme-substrate complex formation of horseradish peroxidase with aromatic donors. both sign and magnitude of the effect depends on galide ligand properties and structure of the donor's molecule. the maximum of the galide complex optical density removes redwise with the galide weight increasing. cloride and bromide bi ...20136855760
[giant raman scattering of alkaline phosphatase, horseradish peroxidase and lactoperoxidase on silver electrodes].surface enhanced raman scattering of three enzymes--alkaline phosphatase, horseradish peroxidase and lactoperoxidase is studied. the intensity of normal vibrations of definite amino acids is determined by their orientation on the surface and depends on the electrode potential. alkaline phosphatase and lactoperoxidase make a complex with silver ions.20133435720
peroxidase-mediated in vitro metabolism of diethylstilbestrol and structural analogs with different biological activities.the comparative peroxidative metabolism of diethylstilbestrol (des) and structurally related compounds of different biological activity was investigated in vitro with horseradish peroxidase (hrp) and hydrogen peroxide (h2o2) as a model peroxidase system: uv spectroscopy revealed the formation of p-quinone intermediates in hrp-h2o2 catalyzed incubations of des, 3',3",5',5"-tetrafluoro-des (tf-des) and dimethylstilbestrol (dms) and the tautomerization of the quinones to their dien compounds, z,z-d ...20134053219
[phenothiazines are slowly oxidizable substrates of horseradish peroxidase].reactions of peroxidase oxidation of triftazine and thioproperazine have been investigated in the presence of horseradish peroxidase using steady state kinetic methods. it has been shown that phenothiazines are slowly oxidizable substrates for horseradish peroxidase. k(cat) and k(m) values have been determined in the range of ph from 4.5 to 7.5. the study of co-oxidation of phenothiazines and o-dianisidine (odn) revealed that in the presence of aminazine and odn in the reaction medium both subst ...201422629605
preparation of graphene nanoplatelet-titanate nanotube composite and its advantages over the two single components as biosensor immobilization materials.a novel nanocomposite consisting of graphene nanoplatelets (gnps) and titanate nanotubes (tnts) have been synthesized successfully utilizing the hydrothermal method. the gnp-tnt composite was characterized by transmission electron microscopy, x-ray diffraction, fourier transform infrared spectroscopy and electrochemical impedance spectroscopy. the voltammetric characterization of gnp-tnt composite, pure gnps and pure tnts modified horseradish peroxidase (hrp) biosensors were conducted to select ...201423939473
electrochemical immunoassay for procalcitonin antigen detection based on signal amplification strategy of multiple nanocomposites.procalcitonin, as a medium of inflammation, has become a new marker of the identification of severe bacterial infections in recent years and has received high attention due to its most ideal diagnostic indicators of specificity with major types of organism systemic inflammation of bacterial infection in the early stages. thus, a novel method for the determination of procalcitonin (pct) was developed based on a sandwich-type electrochemical immunosensor, which combined a simple immunosensor array ...201423978454
[calcium-binding proteins and cytochrome oxidase activity in the turtle optic tectum characterzing the visual tectofugal pathway].using immunohistochemistry and tracer technique, we studied in the optic tectum of turtles (emys orbicularis and testudo horsfieldi) the distribution of cabpr (parvalbumin, pv, calbindin, cb, calretinin, cr) before and after horseradish peroxidase delivery into nucleus rotundus (rot). in parallel, activity of cytochrome oxidase (co) was studied. in the main link of the tectofugal visual pathway (the central gray layer, sgc) in the both chelonian species rare pv-ir, as well cb- and cr-ir neurons ...201425507791
[calcium-binding proteins and cytochrome oxidase activity in the turtle optic tectum characterzing the visual tectofugal pathway].using immunohistochemistry and tracer technique, we studied in the optic tectum of turtles (emys orbicularis and testudo horsfieldi) the distribution of cabpr (parvalbumin, pv, calbindin, cb, calretinin, cr) before and after horseradish peroxidase delivery into nucleus rotundus (rot). in parallel, activity of cytochrome oxidase (co) was studied. in the main link of the tectofugal visual pathway (the central gray layer, sgc) in the both chelonian species rare pv-ir, as well cb- and cr-ir neurons ...201425434193
effect of ionic liquids alkyl chain length on horseradish peroxidase thermal inactivation kinetics and activity recovery after inactivation.the effects of different alkyl chain lengths of ionic liquids 1-ethyl-, 1-butyl- and 1-hexyl-3-methylimidazolium chloride, on the catalytic activity, thermal stability and deactivation kinetics of horseradish peroxidase were studied in the temperature range of 45-85 °c. the presence of 1-ethyl- and 1-butyl-ionic liquids up to 25% (w/v) did not affect significantly the enzyme activity at 25 °c, whereas the addition of 1-hexyl-solvent resulted in lower activity of enzyme. typical biphasic deactiva ...201423990070
ros generation and multiple forms of mammalian mitochondrial glycerol-3-phosphate dehydrogenase.overproduction of reactive oxygen species (ros) has been implicated in a range of pathologies. mitochondrial flavin dehydrogenases glycerol-3-phosphate dehydrogenase (mgpdh) and succinate dehydrogenase (sdh) represent important ros source, but the mechanism of electron leak is still poorly understood. to investigate the ros production by the isolated dehydrogenases, we used brown adipose tissue mitochondria solubilized by digitonin as a model. enzyme activity measurements and hydrogen peroxide p ...201423999537
hollow graphitic nanocapsules as efficient electrode materials for sensitive hydrogen peroxide detection.carbon nanomaterials are typically used in electrochemical biosensing applications for their unique properties. we report a hollow graphitic nanocapsule (hgn) utilized as an efficient electrode material for sensitive hydrogen peroxide detection. methylene blue (mb) molecules could be efficiently adsorbed on the hgn surfaces, and this adsorption capability remained very stable under different ph regimes. hgns were used as three-dimensional matrices for coimmobilization of mb electron mediators an ...201424035356
analytical approaches to determination of carnitine in biological materials, foods and dietary supplements.l-carnitine is a vitamin-like amino acid derivative, which is an essential factor in fatty acid metabolism as acyltransferase cofactor and in energy production processes, such as interconversion in the mechanisms of regulation of cetogenesis and termogenesis, and it is also used in the therapy of primary and secondary deficiency, and in other diseases. the determination of carnitine and acyl-carnitines can provide important information about inherited or acquired metabolic disorders, and for mon ...201424001835
a highly sensitive prostate-specific antigen immunosensor based on gold nanoparticles/pamam dendrimer loaded on mwcnts/chitosan/ionic liquid nanocomposite.we have developed a sensitive electrochemical immunosensor for the detection of prostate-specific antigen (psa), based on covalently immobilizing of anti-psa and redox mediator (thionine) onto gold nanoparticles-incorporated polyamidoamine dendrimer (aunps-pamam) and multiwalled carbon nanotubes/ionic liquid/chitosan nanocomposite (mwcnts/il/chit) as the support platform. the mwcnts/il/chit nanocomposite and synthesized aunps were characterized using sem and tem microscopy techniques. greatly am ...201424016535
fast in situ enzymatic gelation of ppo-peo block copolymer for injectable intraocular lens in vivo.foldable intraocular lenses (iols) have been utilized to substitute natural lens of cataract patients. in this study, we developed a fast, in situ gelable hydrogel requiring no toxic agent as an injectable iol material. a 4-armed ppo/peo-phenol conjugate by a non-degradable linker was synthesized to form a hydrogel in situ by horseradish peroxidase. the gelation time and modulus could be controlled, ranging from 20 s to 2 min and from 1 to 43 kpa. the adhesion of human lens epithelial cells on t ...201424051202
sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification.whole-mount in situ hybridization is the preferred method for detecting transcript distributions in whole embryos, tissues, and organs. we present here a sensitive fluorescent in situ hybridization method for colocalization analysis of different transcripts in whole embryonic zebrafish brains. the method is based on simultaneous hybridization of differently hapten-labeled rna probes followed by sequential rounds of horseradish peroxidase (pod)-based transcript detection. sequential detection inv ...201424048934
dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, schistocerca gregaria.injection of fluorochromes such as alexa fluor(®) 568 into single cells in brain slices reveals a network of dye-coupled cells to be associated with the central complex. subsequent immunolabeling shows these cells to be repo positive/glutamine synthetase positive/horseradish peroxidase negative, thus identifying them as astrocyte-like glia. dye coupling fails in the presence of n-heptanol indicating that dye spreads from cell to cell via gap junctions. a cellular network of dye-coupled, astrocyt ...201424048929
finding the best antibody dilution by repeated immunostaining of the same tissue section.one can find the optimal antibody dilution for immunostaining by repeated staining on the same tissue section by using a less dilute antibody for each attempt. using secondary antibody and horseradish peroxidase conjugated to a dextran polymer, a section stained repeatedly with several dilutions of antibody appears as good as a section stained with only the last dilution.201424047151
a new assay for fast, reliable crim status determination in infantile-onset pompe disease.pompe disease is caused by a deficiency of acid α-glucosidase (gaa; ec, 3.2.1.20), and the infantile-onset form is rapidly fatal if left untreated. however, recombinant human gaa (rhgaa) enzyme replacement therapy (ert) extends survival for infantile pompe patients. although cross-reactive immunologic material (crim)-negative patients, who lack detectable endogenous gaa, mount an immune response to rhgaa that renders the therapy ineffective, timely induction of immune tolerance in these patients ...201424044919
an assay for pro-oxidant reactivity based on phenoxyl radicals generated by laccase.a transient species may be detected with uv-vis and epr spectroscopy during turnover of a laccase with quercetin; this species is assigned as a quercetin-derived radical, based on epr spectra as well the observed uv-vis similarities (a 540nm centred band) with previously reported data. the rates of formation and decay of this species correlate well (r=0.9946) with the pro-oxidant reactivity manifested by flavonoids in the presence of laccase. an assay for the pro-oxidant reactivity of natural pr ...201424054233
a paper-based microfluidic electrochemical immunodevice integrated with amplification-by-polymerization for the ultrasensitive multiplexed detection of cancer biomarkers.a novel signal amplification strategy for ultrasensitive multiplexed detection of cancer biomarkers using a paper-based microfluidic electrochemical immunodevice is described. specifically, a controlled radical polymerization reaction is triggered after the capture of target molecules on the immunodevice surface. growth of long chain polymeric materials provides numerous sites for subsequent horseradish peroxidase (hrp) coupling, which in turn significantly enhances electrochemical signal output ...201424055931
developing new materials for paper-based diagnostics using electrospun nanofibers.the use of electrospun nanofibers as functional material in paper-based lateral flow assays (lfas) was studied. specific chemical features of the nanofibers were achieved by doping the base polymer, poly(lactic acid) (pla), with poly(ethylene glycol) (peg) and polystyrene8k-block-poly(ethylene-ran-butylene)25k-block-polyisoprene10k-brij76 (k3-brij76) (kb). the lfas were assembled such that the sample flowed through the nanofiber mat via capillary action. initial investigations focused on the sus ...201424068171
comparison of lignin peroxidase and horseradish peroxidase for catalyzing the removal of nonylphenol from water.concentrations of aqueous-phase nonylphenol (np), a well-known endocrine-disrupting chemical, are shown to be reduced effectively via reaction with lignin peroxidase (lip) or horseradish peroxidase (hrp) and hydrogen peroxide. we systematically assessed their reaction efficiencies at varying conditions, and the results have confirmed that the catalytic performance of lip toward np was more efficient than that of hrp under experimental conditions. mass spectrum analysis demonstrated that polymeri ...201424062065
amplified colorimetric detection of mercuric ions through autonomous assembly of g-quadruplex dnazyme nanowires.an amplified colorimetric detection of hg(2+) is proposed by combining t-hg(2+)-t base pairs and hybridization chain reaction (hcr). two hairpins consisting of three-fourths and one-fourth of the horseradish peroxidase (hrp)-mimicking dnazyme in inactive configuration are used as functional elements. in the presence of hg(2+), one of the hairpins is opened by an assistant probe with the help of t-hg(2+)-t base pairs and this triggers an autonomous cross-opening of the two hairpins using the stra ...201424060975
characterization of a layered methylene blue/vanadium oxide nanocomposite and its application in a reagentless h2o2 biosensor.layered nanocomposite of methylene blue (mb)-intercalated vanadium oxide was obtained through a simple hydrothermal synthesis method using mb, v2o5, and nai as starting materials. the intercalation reaction was proven to be successful using x-ray diffraction pattern. the mb-v2o5 nanocomposite was characterized using a scanning electron micrograph, infrared spectra, thermogravimetric analysis, uv spectra, and electrochemical measurements. the intercalated mb cations showed a fine diffusion-contro ...201424057304
chitosan-g-hematin: enzyme-mimicking polymeric catalyst for adhesive hydrogels.phenol derivative-containing adhesive hydrogels has been widely recognized as having potential for biomedical applications, but their conventional production methods, utilizing a moderate/strong base, alkaline buffers, the addition of oxidizing agents or the use of enzymes, require alternative approaches to improve their biocompatibility. in this study, we report a polymeric, enzyme-mimetic biocatalyst, hematin-grafted chitosan (chitosan-g-hem), which results in effective gelation without the us ...201424071001
multidisciplinary approach to study the effect of water status and mobility on the activity of peroxidase in solutions.the effect of water mobility on horseradish peroxidase (hrp) activity in solutions was investigated by measuring water activity (aw), freezable water content, (1)h proton transverse relaxation time and water self-diffusivity determined by nuclear magnetic resonance. the effect of system mobility as described by viscosity and glass transition temperature (t'g) was also studied. the aw and viscosity of aqueous solutions were modulated using ligands (glucose, sorbitol and trehalose) and a thickener ...201424099539
an amplex red-based fluorometric and spectrophotometric assay for l-asparaginase using its natural substrate.we report on the development of a sensitive real-time assay for monitoring the activity of l-asparaginase that hydrolyzes l-asparagine to l-aspartate and ammonia. in this method, l-aspartate is oxidized by l-aspartate oxidase to iminoaspartate and hydrogen peroxide (h2o2), and in the detection step horseradish peroxidase uses h2o2 to convert the colorless, nonfluorescent reagent amplex red to the red-colored and highly fluorescent product resorufin. the assay was validated in both the absorbance ...201424113285
triple signal amplification using gold nanoparticles, bienzyme and platinum nanoparticles functionalized graphene as enhancers for simultaneous multiple electrochemical immunoassay.here we demonstrated an ultrasensitive electrochemical immunoassay employing graphene, platinum nanoparticles (ptnps), glucose oxidase (god) and horseradish peroxidase (hrp) as enhancers to simultaneously detect carcinoembryonic antigen (cea) and alpha-fetoprotein (afp). this immunosensor is based on the observation that multiple-labeled antibodies (thionine-labeled anti-cea and ferrocene-labeled anti-afp) recognition event yielded a distinct voltammetric peak through "sandwich" immunoreaction, ...201424113435
estrogen reduces endoplasmic reticulum stress to protect against glucotoxicity induced-pancreatic β-cell death.estrogen can improve glucose homeostasis not only in diabetic rodents but also in humans. however, the molecular mechanism by which estrogen prevents pancreatic β-cell death remains unclear. to investigate this issue, ins-1 cells, a rat insulinoma cell line, were cultured in medium with either 11.1mm or 40mm glucose in the presence or the absence of estrogen. estrogen significantly reduced apoptotic β-cell death by decreasing nitrogen-induced oxidative stress and the expression of the er stress ...201424120916
the role of survivin in thyroid tumors: differences of expression in well-differentiated, non-well-differentiated, and anaplastic thyroid cancers.survivin is involved in human cancer and is responsible for aggressive biological behavior and poor clinical outcomes in several human malignancies. thus, we hypothesized that the upregulation of survivin protein expression may be enhanced in parallel with transition toward a poorly differentiated phenotype in human thyroid carcinomas.201424117205
antioxidant action of 7,8-dihydroxyflavone protects pc12 cells against 6-hydroxydopamine-induced cytotoxicity.oxidative stress-induced neuronal death plays a pivotal role in pathogenesis of neurodegenerative disorders. recently, 7,8-dihydroxyflavone (7,8-dhf) has been shown to exert neuroprotective effects by acting as a selective tyrosine kinase receptor b (trkb) agonist. in addition, the antioxidant action of 7,8-dhf may protect neuronal cells against oxidative injury. in the present study, we used pc12 cells, a cell line generally thought to lack trkb, to investigate the effect of 7,8-dhf on 6-hydrox ...201424220540
regeneration of optic nerve fibers with unoprostone, a prostaglandin-related antiglaucoma drug, in adult cats.we investigated the effects of unoprostone on neurite extension of cultured retinal pieces and axonal regeneration of retinal ganglion cells in the crushed optic nerve of adult cats.201424129676
in vivo modulation of polo-like kinases supports a key role for plk2 in ser129 α-synuclein phosphorylation in mouse brain.α-synuclein is the major component of lewy bodies. α-synuclein phosphorylated at ser 129 (phospho-α-syn) is the most common synuclein modification observed in parkinson's disease pathology and transgenic animal models. polo-like kinase 2 (plk2) was previously proposed as an important kinase in α-synuclein phosphorylation at ser129. to better understand the role of plk2 in α-synuclein phosphorylation in vivo, we further evaluated the effect of plk2 genetic knockdown and pharmacological inhibition ...201424128992
seasonal lectin binding variations of thumb pad in the frog (pelophylax ridibundus).the thumb pad is one of the most common secondary sexual characteristics in frogs. although it is known that amphibian skin has affinity for several lectins, there is no report regarding lectin-binding affinity of the thumb pad or its structural components. this study investigated localization and seasonal variation of specific carbohydrate moieties of glycoconjugates in both the epidermal and dermal components of the frog thumb pad at the light microscopic level using lectin histochemistry. the ...201424127244
target-induced self-assembly of dna nanomachine on magnetic particle for multi-amplified biosensing of nucleic acid, protein, and cancer cell.a biosensing system is established for the multi-amplified detection of dna or specific substrates of aptamers under isothermal conditions, which combines nicked rolling circle amplification (n-rca) and beacon assisted amplification (baa) with sensitive colorimetric technique by using dnazymes as reporter units. according to the configuration, the analysis of dna is accomplished by recognizing the target to capture nucleic acid-functionalized magnetic particles, followed by the self-assembly of ...201424140870
integrating bipolar electrochemistry and electrochemiluminescence imaging with microdroplets for chemical analysis.here we develop a microdroplet sensor based on bipolar electrochemistry and electrochemiluminescence (ecl) imaging. the sensor was constructed with a closed bipolar cell on a hybrid poly(dimethylsioxane) (pdms)-indium tin oxide (ito) glass microchip. the ito microband functions as the bipolar electrode and its two poles are placed in two spatially separate micro-reservoirs predrilled on the pdms cover. after loading microliter-sized liquid droplets of tris(2,2'-bipyridyl) ruthenium (ii)/2-(dibut ...201424140829
correlations between blood-brain barrier disruption and neuroinflammation in an experimental model of penetrating ballistic-like brain injury.abstract blood-brain barrier (bbb) disruption is a pathological hallmark of severe traumatic brain injury (tbi) and is associated with neuroinflammatory events contributing to brain edema and cell death. the goal of this study was to elucidate the profile of bbb disruption after penetrating ballistic-like brain injury (pbbi) in conjunction with changes in neuroinflammatory markers. brain uptake of biotin-dextran amine (bda; 3 kda) and horseradish peroxidase (hrp; 44 kda) was evaluated in rats at ...201424138024
effects of intestinal bacteria-derived p-cresyl sulfate on th1-type immune response in vivo and in vitro.protein fermentation by intestinal bacteria generates various compounds that are not synthesized by their hosts. an example is p-cresol, which is produced from tyrosine. patients with chronic kidney disease (ckd) accumulate high concentrations of intestinal bacteria-derived p-cresyl sulfate (pcs), which is the major metabolite of p-cresol, in their blood, and this accumulation contributes to certain ckd-associated disorders. immune dysfunction is a ckd-associated disorder that frequently contrib ...201424161588
intracellular nogo-a facilitates initiation of neurite formation in mouse midbrain neurons in vitro.nogo-a is a transmembrane protein originally discovered in myelin, produced by postnatal cns oligodendrocytes. nogo-a induces growth cone collapse and inhibition of axonal growth in the injured adult cns. in the intact cns, nogo-a functions as a negative regulator of growth and plasticity. nogo-a is also expressed by certain neurons. neuronal nogo-a depresses long-term potentiation in the hippocampus and modulates neurite adhesion and fasciculation during development in mice. here we show that n ...201424157929
injectable 3d hydrogel scaffold with tailorable porosity post-implantation.since rates of tissue growth vary significantly between tissue types, and also between individuals due to differences in age, dietary intake, and lifestyle-related factors, engineering a scaffold system that is appropriate for personalized tissue engineering remains a significant challenge. in this study, a gelatin-hydroxyphenylpropionic acid/carboxylmethylcellulose-tyramine (gtn-hpa/cmc-tyr) porous hydrogel system that allows the pore structure of scaffolds to be altered in vivo after implantat ...201424151286
mechanisms of enhanced neutralization of botulinum neurotoxin by monoclonal antibodies conjugated to antibodies specific for the erythrocyte complement receptor.immune complexes formed between monoclonal antibodies (mabs) and toxins can neutralize toxicity in vivo by multiple mechanisms. toxin sequestration and clearance by mabs may be improved by enhancing their ability to bind to red blood cells (rbcs) through immune adherence. this can be achieved by converting the mabs to heteropolymers (hps), which are antigen-specific mabs cross-linked to mabs targeting the complement receptor (cr1), a protein that is expressed on the surface of rbcs in primates a ...201424184879
quantification of cytochrome 2e1 in human liver microsomes using a validated indirect elisa.cyp2e1 is an important cytochrome p450 isoform in many endogenous processes and in the metabolism of organic solvents, a number of drugs and pre-carcinogens. information on the abundance of the enzyme may be valuable in various types of research in the field of toxicology and pharmacology. an indirect elisa for the quantification of cyp2e1 in human liver microsomes was developed and successfully validated. all samples, including validation samples and calibrators, were diluted to a final concent ...201424184659
retinal ganglion cells in the pacific redfin, tribolodon brandtii dybowski, 1872: morphology and diversity.we studied the morphology and diversity of retinal ganglion cells in the pacific redfin, tribolodon brandtii. these cells were retrogradely labeled with horseradish peroxidase and examined in retinal whole mounts. a sample of 203 cells was drawn with a camera lucida. a total of 19 structural parameters were estimated for each cell, and a variety of clustering algorithms were used to classify the cells. the optimal solution was determined by using silhouette analysis. it was based on three variab ...201424178784
efficient sirna delivery and tumor accumulation mediated by ionically cross-linked folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate nanoparticles: for the potential targeted ovarian cancer gene therapy.for effective ovarian cancer gene therapy, systemic administrated tumor-targeting sirna/folic acid-poly(ethylene glycol)-chitosan oligosaccharide lactate (fa-peg-col) nanoparticles is vital for delivery to cancer site(s). sirna/fa-peg-col nanoparticles were prepared by ionic gelation for effective fa receptor-expressing ovarian cancer cells transfection and in vivo accumulation. the chemical structure of fa-peg-col conjugate was characterized by maldi-tof-ms, ft-ir and (1)h nmr. the average size ...201424178005
novel electrochemical aptamer biosensor based on an enzyme-gold nanoparticle dual label for the ultrasensitive detection of epithelial tumour marker muc1.a novel platform based on a hairpin oligonucleotide (ho) switch, gold nanoparticles (aunps), and enzyme signal amplification for the ultrasensitive detection of mucin 1 protein (muc1) was developed in this assay. this ho aptamers and horseradish peroxidase (hrp) were immobilised on the aunps to yield ho-aunp-hrp conjugates. aunps were used as labels and bridges between the ho and hrp. hrp was also used as label for catalysing the oxidation of o-phenylenediamine by h2o2. the reaction product was ...201424189297
multi-enzyme co-embedded organic-inorganic hybrid nanoflowers: synthesis and application as a colorimetric sensor.this study reports a facile method for the synthesis of multi-enzyme co-embedded organic-inorganic hybrid nanoflowers, using glucose oxidase (gox) and horseradish peroxidase (hrp) as the organic components, and cu3(po4)2 · 3h2o as the inorganic component. the synthesized nanoflowers enable the combination of a two-enzyme cascade reaction in one step, in which the gox component of the nanoflowers oxidizes glucose to generate h2o2, which then reacts with the adjacent hrp component on the nanoflowe ...201424186239
differential up-regulation of vesl-1/homer 1 protein isoforms associated with decline in visual performance in a preclinical glaucoma model.glaucoma is a multifactorial progressive ocular pathology, clinically presenting with damage to the retina and optic nerve, ultimately leading to blindness. retinal ganglion cell loss in glaucoma ultimately results in vision loss. vesl/homer proteins are scaffolding proteins that are critical for maintaining synaptic integrity by clustering, organizing and functionally regulating synaptic proteins. current anti-glaucoma therapies target iop as the sole modifiable clinical parameters. long-term p ...201424219919
gaba-induced uncoupling of gaba/benzodiazepine site interactions is mediated by increased gabaa receptor internalization and associated with a change in subunit composition.persistent activation of gabaa receptors triggers compensatory changes in receptor function that are relevant to physiological, pathological and pharmacological conditions. chronic treatment of cultured neurons with gaba for 48h has been shown to produce a down-regulation of receptor number and an uncoupling of gaba/benzodiazepine site interactions with a half-time of 24-25h. down-regulation is the result of a transcriptional repression of gabaa receptor subunit genes and depends on activation o ...201424215979
expression of β1- and β2-adrenoceptors in different subtypes of interneurons in the medial prefrontal cortex of mice.noradrenaline acting via β-adrenoceptors (β-ars) in the cns plays an important role in learning/memory and cognitive functions. β-ars have been shown to be expressed in cortical pyramidal and subcortical principal cells. however, little is known about β-ar expression in different subtypes of gabaergic neurons. here, we report that both β1- and β2-ars are expressed in a majority of gabaergic interneurons in the medial prefrontal cortex of mice, including parvalbumin (pv)-, calretinin (cr)-, calbi ...201424215978
differential cortical neurotrophin and cytogenetic adaptation after voluntary exercise in normal and amnestic rats.voluntary exercise (vex) has profound effects on neural and behavioral plasticity, including recovery of cns trauma and disease. however, the unique regional cortical adaption to vex has not been elucidated. in a series of experiments, we first examined whether vex would restore and retain neurotrophin levels in several cortical regions (frontal cortex [fc], retrosplenial cortex [rsc], occipital cortex [oc]) in an animal model (pyrithiamine-induced thiamine deficiency [ptd]) of the amnestic diso ...201424215977
direct electron transfer of horseradish peroxidase on a functional nanocomplex modified glassy carbon electrode.direct electron transfer of horseradish peroxidase (hrp) was achieved by immobilizing hrp on a functional nanocomplex modified glassy carbon (gc) electrode. the cyclic voltammograms (cvs) of the modified electrode have a pair of well-defined redox peaks with a formal potential (e°') of -26 ± 2 mv versus ag/agcl, in 0.05 m, ph7.0 phosphate buffer solution (pbs) at a scan rate of 0.05 v/s. the heterogeneous electron transfer constant (ks) was calculated to be 1.94 s-1. the modified electrode respo ...201424211999
measurement of salivary cortisol by a chemiluminescent organic-based immunosensor.a highly sensitive chemiluminescent immunoassay (clia) using a sensitive organic photodetector was developed to detect human cortisol, an important biomarker for stress-related diseases. the developed clia was performed onto gold-coated glass chips, on which anti-cortisol antibodies were immobilised and chemiluminescent horseradish peroxidase-luminol-peroxide reactions were generated. using cortisol-spiked artificial saliva samples, the clia biosensor showed a linear range of detection between 0 ...201424211877
microrna-101 inhibits human hepatocellular carcinoma progression through ezh2 downregulation and increased cytostatic drug sensitivity.oncogene polycomb group protein enhancer of zeste homolog 2 (ezh2) has been proposed to be a target gene of putative tumor suppressor microrna-101 (mir-101). the aim of our study was to investigate the functional role of both mir-101 and ezh2 in human hepatocellular carcinoma (hcc).201424211739
selective vulnerability of hippocampal sub-fields to oxygen-glucose deprivation is a function of animal age.for more than a century, the hippocampal sub-fields have been recognized as being differentially vulnerable to injury. while the cause remains unknown, the explanations generally considered have involved either vascular differences, or innate variability among cells. to examine the latter possibility, we prepared acute hippocampal slices from sprague-dawley rats, applied a brief period of oxygen-glucose deprivation (ogd; an in vitro model of ischemia), and assessed the viability of dissected sub ...201424211659
one-electron oxidation of diclofenac by human cytochrome p450s as a potential bioactivation mechanism for formation of 2'-(glutathion-s-yl)-deschloro-diclofenac.reactive metabolites have been suggested to play a role in the idiosyncratic hepatotoxicity observed with diclofenac (df). by structural identification of the gsh conjugates formed after p450-catalyzed bioactivation of df, it was shown that three types of reactive intermediates were formed: p-benzoquinone imines, o-imine methide and arene-oxide. recently, detection of 2'-(glutathion-s-yl)-deschloro-diclofenac (ddf-sg), resulting from chlorine substitution, suggested the existence of a fourth typ ...201424246759
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