Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human microbial ecology: lactobacilli, probiotics, selective decontamination. | health care-associated infections are closely associated with different medical interventions which interrupt the balance of human microbiota. the occasional predominance of opportunistic pathogens may lead to their translocation into the lymph nodes and bloodstream, causing endogenous (primary or secondary) hospital infections. the question is raised as to if there is a possibility for prevention of the imbalance of gi microbiota during medical interventions in critically ill patients. prophyla ... | 2011 | 21787875 |
| dissimilar properties of two recombinant lactobacillus acidophilus displaying salmonella flic with different anchoring motifs. | cell-surface display of heterologous antigens is considered a useful technique for vaccine-delivery by recombinant lactobacilli. in this study, two recombinant lactobacillus acidophilus derivatives displaying salmonella flagellin (flic) were constructed using different anchor motifs. in one instance the flic protein was fused to the c-terminal region of a cell envelope proteinase (prtp) and bound to the cell wall by electrostatic bonds. in the other case the same antigen was conjugated to the an ... | 2011 | 21784918 |
| characterization of novel glycolipid antigens with an {alpha}-galactose epitope in lactobacilli detected with rabbit anti-lactobacillus antisera and occurrence of antibodies against them in human sera. | anti-lactobacillus johnsonii (lj) antisera generated by immunization of rabbits with lj reacted with glyceroglycolipids in lj, i.e. dihexaosyl diacylglycerol (dh-dg), trihexaosyl dg (th-dg), and tetrahexaosyl dg (teth-dg), whose reactivities with antisera increased proportionally with longer carbohydrate chains of glycolipids. structural analyses of glycolipids from lj revealed that dh-dg was gala1-2glca1-3'dg, and th-dg and teth-dg were novel derivatives of it with a-gal at the nonreducing term ... | 2011 | 21784785 |
| the quorum sensing luxs gene is induced in lactobacillus acidophilus ncfm in response to listeria monocytogenes. | the luxs gene involved in quorum sensing has been shown to control different behaviour of probiotic lactobacilli. in this study we investigated if luxs in lactobacillus acidophilus ncfm was up-regulated in response to listeria monocytogenes egd-e. the two bacterial strains were grown in mono- and co-culture and the growth of both bacteria and the transcriptional level of luxs in l. acidophilus cells were monitored. contrary to l. acidophilus, the growth of l. monocytogenes was significantly affe ... | 2011 | 21784546 |
| intravaginal microbial flora by the 16s rrna gene sequencing. | objective: conventional diagnosis of bacterial vaginosis contains some controversial points. to understand accurately the relationship between clinical stages and the microbiotas, the intravaginal microbial flora was analyzed by the clone library method. study design: vaginal fluid samples from 31 patients were examined. lactobacillary grade, nugent score, culture-based method, and clone library analysis using the 16srrna gene sequencing were performed and were compared with each other. results: ... | 2011 | 21783170 |
| plantaricin a synthesized by lactobacillus plantarum induces in vitro proliferation and migration of human keratinocytes and increases the expression of tgf-ß1, fgf7, vegf-a and il-8 genes. | this work showed the effect of pheromone plantaricin a (plna) on the proliferation and migration of the human keratinocytes nctc 2544. plna was chemically synthesized and used as pure peptide or biologically synthesized during co-cultivation of lactobacillus plantarum dc400 and lactobacillus sanfranciscensis dppma174. the cell-free supernatant (cfs) was used as the crude preparation containing plna. the inductive effect of plna on the proliferation of nctc 2544 cells was higher than that found f ... | 2011 | 21782870 |
| transcriptome analysis of probiotic lactobacillus casei zhang during fermentation in soymilk. | lactobacillus casei zhang is a widely recognized probiotic bacterium, which is being commercially used in china. to study the gene expression dynamics of l. casei zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late logarithmic phase, and the stationary phase. comparisons of different transcripts next to each other revealed 162 and 63 significantly induced genes in the late logarithmic phase an ... | 2011 | 21779970 |
| the effect of temperature and ph gradients on lactobacillus rhamnosus gene expression of stress-related genes. | in this study, lactobacillus rhamnosus, a renowned probiotic, was cultivated in fluctuating environment. base gradients caused by a ph control in an industrial process and temperature gradients caused by uneven heating were simulated with a scale-down method. a ph gradient was created in a plug flow reactor (pfr). expression of ph stress-related genes (atpa, aldb, cfa, groel, hrca and psts) were studied as a relative gene expression study using ldhd as a reference gene. expression measurements w ... | 2011 | 21779891 |
| metabolic fate of orally administered enzymatically synthesized glycogen in rats. | we developed a new process for enzymatically synthesized glycogen (esg), which is equivalent in physicochemical properties to natural-source glycogen (nsg) except its resistant property to degradation by a-amylase in vitro. in this study the metabolic fates of orally administered esg in rats were investigated by a single oral administration test and a 2 week ingestion test. the glycemic index of esg was 79. after the 2 week ingestion of esg, the cecal content and production of short chain fatty ... | 2011 | 21779577 |
| the starting lineup: key microbial players in intestinal immunity and homeostasis. | the complexity of microbiota inhabiting the intestine is increasingly apparent. delicate balance of numerous bacterial species can affect development of the immune system, how susceptible a host is to pathogenic organisms, and the auto-inflammatory state of the host. in the last decade, with the increased use of germ-free mice, gnotobiotic mice, and animal models in which a germ-free animal has been colonized with a foreign microbiota such as humanized mice, it has been possible to delineate rel ... | 2011 | 21779278 |
| in vitro mutagen binding and antimutagenic activity of human lactobacillus rhamnosus 231. | in vitro mutagen binding ability of human lactobacillus rhamnosus 231 (lr 231) was evaluated against acridine orange (ao), n-methyl-n'-nitro-n-nitrosoguanidine (mnng), 2-amino-3, 8-dimethylimidazo-[4,5-f]-quinoxaline (meiqx) and 4-nitro-o-phenylenediamine (npd). binding of ao by lr 231 is due to adsorption, thereby leading to removal of mutagen in solution and is instantaneous, ph- and concentration-dependent. whereas, binding of mnng and meiqx by lr 231 results into biotransformation leading to ... | 2011 | 21777684 |
| functional identification in lactobacillus reuteri of a pocr-like transcription factor regulating glycerol utilization and vitamin b12 synthesis. | abstract: background: lactobacillus reuteri harbors the genes responsible for glycerol utilization and vitamin b12 synthesis within a genetic island phylogenetically related to gamma-proteobacteria. within this island, resides a gene (lreu_1750) that based on its genomic context has been suggested to encode the regulatory protein pocr and presumably control the expression of the neighboring loci. however, this functional assignment is not fully supported by sequence homology, and hitherto, compl ... | 2011 | 21777454 |
| methodological issues in randomized trials assessing probiotics for periodontal treatment. | dhingra k. methodological issues in randomized trials assessing probiotics for periodontal treatment. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01399.x.©2011 john wiley & sons a/s background and objective: probiotics traditionally used in medicine field are now being used in an attempt to control and treat periodontal disease. however, the trials used to analyze the effects of probiotics have been subject to methodological criticism. the aim of this review was to assess the methodolog ... | 2011 | 21777405 |
| enhanced growth of lactobacilli and bioconversion of isoflavones in biotin-supplemented soymilk upon ultrasound-treatment. | this study aimed at utilizing ultrasound treatment to further enhance the growth of lactobacilli and their isoflavone bioconversion activities in biotin-supplemented soymilk. strains of lactobacilli (lactobacillus acidophilus bt 1088, l. fermentum bt 8219, l. acidophilus ftdc 8633, l. gasseri ftdc 8131) were treated with ultrasound (30khz, 100w) at different amplitudes (20%, 60% and 100%) for 60, 120 and 180s prior to inoculation and fermentation in biotin-soymilk. the treatment affected the fat ... | 2011 | 21775184 |
| technological characterization and survival of the exopolysaccharide-producing strain lactobacillus delbrueckii subsp. lactis 193 and its bile-resistant derivative 193+ in simulated gastric and intestinal juices. | the capacity of lactic acid bacteria to produce exopolysaccharides (eps) conferring microorganisms a ropy phenotype could be an interesting feature from a technological point of view. progressive adaptation to bile salts might render some lactobacilli able to overcome physiological gut barriers but could also modify functional properties of the strain, including the production of eps. in this work some technological properties and the survival ability in simulated gastrointestinal conditions of ... | 2011 | 21774862 |
| immunomodulatory activity of lactobacillus strains isolated from fermented vegetables and infant stool. | four lactobacillus strains - lactobacillus plantarum cjlp133, l. plantarum cjlp243, l. plantarum cjnr26, and lactobacillus gasseri cjmf3 - were isolated from korean fermented food or healthy infant feces, and their capacity to modulate cellular and humoral immune responses was studied. feeding of the tested lactobacilli for 8 weeks did not alter the weight of and cell numbers in the spleen of mice. however, cjlp133 and cjlp243 strains increased the t lymphocyte population in the spleen of mice, ... | 2011 | 21774581 |
| [in process citation]. | 2011 | 21774182 | |
| chicory inulin does not increase stool weight or speed up intestinal transit time in healthy male subjects. | inulin is a non-digestible oligosaccharide classified as a prebiotic, a substrate that promotes the growth of certain beneficial microorganisms in the gut. we examined the effect of a 20 g day(-1) supplement of chicory inulin on stool weight, intestinal transit time, stool frequency and consistency, selected intestinal microorganisms and enzymes, fecal ph, short chain fatty acids and ammonia produced as by-products of bacterial fermentation. twelve healthy male volunteers consumed a well-defined ... | 2011 | 21773588 |
| microbial contamination of fuel ethanol fermentations. | microbial contamination is a pervasive problem in any ethanol fermentation system. these infections can at minimum affect the efficiency of the fermentation and at their worse lead to stuck fermentations causing plants to shut down for cleaning before beginning anew. these delays can result in costly loss of time as well as lead to an increased cost of the final product. lactic acid bacteria (lab) are the most common bacterial contaminants found in ethanol production facilities and have been lin ... | 2011 | 21770989 |
| prebiotic fibres dose-dependently increase satiety hormones and alter bacteroidetes and firmicutes in lean and obese jcr:la-cp rats. | there is a growing interest in modulating gut microbiota with diet in the context of obesity. the purpose of the present study was to evaluate the dose-dependent effects of prebiotics (inulin and oligofructose) on gut satiety hormones, energy expenditure, gastric emptying and gut microbiota. male lean and obese jcr:la-cp rats were randomised to either of the following: lean 0 % fibre (lc), lean 10 % fibre (lf), lean 20 % fibre (lhf), obese 0 % fibre (oc), obese 10 % fibre (of) or obese 20 % fibr ... | 2011 | 21767445 |
| probiotics for persistent diarrhea in children. | 2011 | 21766752 | |
| lactobacillus reuteri atcc 55730 and l22 display probiotic potential in vitro and protect against salmonella-induced pullorum disease in a chick model of infection. | lactobacillus reuteri atcc 55730 (l. reuteri atcc 55730) and l. reuteri l22 were studied for their probiotic potential. these two strains were able to produce an antimicrobial substance, termed reuterin, the maximum production of reuterin by these two strains was detected in the late logarithmic growth phase (16h in mrs and 20h in lb broths). these two strains could significantly reduce the growth of salmonella pullorum atcc 9120 in mrs broth, l. reuteri atcc 55730 with a reduction of 48.2±4.15% ... | 2011 | 21764090 |
| design of nanoemulsion-based delivery systems of natural antimicrobials: effect of the emulsifier. | this work aims at investigating the effect of the nanoemulsion delivery systems on the antimicrobial activity of different essential oil components. carvacrol, limonene and cinnamaldehyde were encapsulated in the sunflower oil droplets of nanoemulsions prepared by high pressure homogenization and stabilized by different emulsifiers: (a) lecithin, (b) pea proteins, (c) sugar ester and (d) a combination of tween 20 and glycerol monooleate. the antimicrobial activity was measured against three diff ... | 2011 | 21763730 |
| cdna-aflp to study the transcriptional responses of lactobacillus rhamnosus growing in cheese-like medium. | aims: lactobacillus rhamnosus is a dominant species during parmigiano reggiano cheese ripening and exhibits a great adaptability to unfavorable growth conditions. gene expression of a lact. rhamnosus, isolated from parmigiano reggiano cheese, grown in a rich medium (mrs) and in a cheese-like medium (cb) has been compared by a novel cdna amplified fragment length polymorphism (cdna-aflp) protocol. methods and results: two techniques, capillary and gel electrophoresis cdna-aflp, were applied to ... | 2011 | 21762473 |
| evaluation of waste chicken feathers as peptone source for bacterial growth. | aims: peptones are one of the most expensive constituents of microbial media. this study was undertaken to prepare the peptone from waste chicken feathers through a new process. methods and results: the chemical analysis of chicken feather peptone (cfp) was performed. the ability of cfp to support the growth of the three test bacteria in liquid and agar media was comparable to those of three commercial peptones [tryptone peptone (tp), fish peptone and protease peptone (pp)]. conclusions: cfp ... | 2011 | 21762471 |
| lactobacillus rhamnosus gg as an effective probiotic for murine giardiasis. | the gut microflora is an important constituent in the intestinal mucosal barrier and has been introduced as the concept of probiotic therapy that beneficially affects the host by improving its intestinal microbial balance. therefore, the main objective of the study was to explore the protective potential of various lactobacilli strains for murine giardiasis. by experimentation, it was found that the probiotic supplementation of either lactobacillus casei, l. acidophilus, l. plantarum, or l. rham ... | 2011 | 21760784 |
| effect of food models and low-temperature storage on the adhesion of lactobacillus rhamnosus gg to caco-2 cells. | this study evaluated the effects of fat and sugar levels on the surface properties of lactobacillus rhamnosus gg during storage in food model systems, simulating yogurt and ice cream, and related them with the ability of the bacterial cells to adhere to caco-2 cells. freeze-dried l. rhamnosus gg cells were added to the model food systems and stored for 7 days. the bacterial cells were analyzed for cell viability, hydrophobicity, ? potential, and their ability to adhere to caco-2 cells. the resul ... | 2011 | 21756003 |
| proteome reference map of lactobacillus acidophilus ncfm and quantitative proteomics towards understanding the prebiotic action of lactitol. | lactobacillus acidophilus ncfm is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits to the host. lactitol is a synthetic sugar alcohol used as a sugar replacement in low calorie foods and selectively stimulating growth of l. acidophilus ncfm. in the present study the whole-cell extract proteome of l. acidophilus ncfm grown on glucose until late exponential phase was resolved by 2-de (ph 3-7). a total of 275 unique proteins assigned to vario ... | 2011 | 21751373 |
| effect of heat-inactivated kefir-isolated lactobacillus kefiranofaciens m1 on preventing an allergic airway response in mice. | in this study, we assessed the anti-asthmatic effects of heat-inactivated lactobacillus kefiranofaciens m1 (hi-m1) and its fermented milk using different feeding procedures and at various dosage levels. the possible mechanisms whereby hi-m1 has anti-allergic asthmatic effects were also evaluated. ovalbumin (ova)-allergic asthma mice that have been orally administrated the hi-m1 samples showed strong inhibition of production of t helper cell (th) 2 cytokines, pro-inflammatory cytokines, and th17 ... | 2011 | 21749079 |
| characterization of the cysk2-ctl1-cyse2 gene cluster involved in sulfur metabolism in lactobacillus casei. | the up- and downstream regions of ctl1 and ctl2 that encode a cystathionine lyase were analyzed in various lactobacillus casei strains. ctl1 and ctl2 were found to be part of a gene cluster encoding two other open reading frames. one of the two open reading frames precedes ctl1 and encodes a putative cysteine synthase. the other open reading frame lies downstream of ctl1 and encodes a putative serine acetyltransferase. the gene cluster is not present in the publicly available genome sequences of ... | 2011 | 21745695 |
| persistence of microbial and chemical pig manure markers as compared to faecal indicator bacteria survival in freshwater and seawater microcosms. | natural seawater and freshwater microcosms inoculated with pig manure were set up to determine the persistence of pig faecal microbial and chemical markers in these two types of surface water. the concentrations of lactobacillus amylovorus, the bacteroidales pig-2-bac 16s rrna genetic marker, five stanols and the evolution of two ratios of stanols, r(1) (coprostanol to the sum of coprostanol and 24-ethylcoprostanol) and r(2) (sitostanol to coprostanol) were analyzed during two months along with ... | 2011 | 21745675 |
| [in process citation]. | 2011 | 21744644 | |
| anaerobic bacteria colonizing the lower airways in lung cancer patients. | anaerobes comprise most of the endogenous oropharyngeal microflora, and can cause infections of airways in lung cancer patients who are at high risk for respiratory tract infections. the aim of this study was to determine the frequency and species diversity of anaerobes in specimens from the lower airways of lung cancer patients. sensitivity of the isolates to conventional antimicrobial agents used in anaerobe therapy was assessed. respiratory secretions obtained by bronchoscopy from 30 lung can ... | 2011 | 21744326 |
| efflux of bile acids in lactobacillus reuteri is mediated by atp. | purpose of work: to study whether an active bile acid (ba) efflux occurs in lactobacillus reuteri crl 1098 as well as the nature (atp or proton motive force [pmf] mediated primary transport) of the ba extrusion mechanism. bas are powerful detergents which disorganize the lipid bilayer structure of cellular membranes. specific bile resistance mechanisms (bile efflux, bile salt hydrolysis, and intrinsic architecture and composition of cell membrane the most prevalent) have been described in intest ... | 2011 | 21744273 |
| discriminant ability for caries risk of modified colorimetric tests. | objetive: the aim of this study was to evaluate the relationship between the caries risk in children over a two-year period and their baseline caries status, salivary levels of mutans streptococci and lactobacilli, and results of the alban test and modifications thereof using different substrates. study design: ninety-five children aged 6-7 were examined in granada (southern spain) for dental caries at baseline and every six months. stimulated saliva was sampled and inoculated in 7 colorimetric ... | 2011 | 21743394 |
| a molecular design that stabilizes active state in bacterial allosteric l-lactate dehydrogenases. | l-lactate dehydrogenase (lldh) of lactobacillus casei (lcldh) is a typical bacterial allosteric lldh that requires fructose 1,6-bisphosphate (fbp) for its enzyme activity. a mutant lcldh was designed to introduce an inter-subunit salt bridge network at the q-axis subunit interface, mimicking lactobacillus pentosus non-allosteric lldh (lpldh). the mutant lcldh exhibited high catalytic activity with hyperbolic pyruvate saturation curves independently of fbp, and virtually the equivalent k(m) and v ... | 2011 | 21828088 |
| regulation of the il-10/il-12 axis in human dendritic cells with probiotic bacteria. | in this study, we have used monocyte-derived dendritic cells (dcs) to design a screening model for the selection of microorganisms with the ability to suppress dc-secreted il-12p70, a critical cytokine for the induction of t-helper cell type 1 immune responses under inflammatory conditions. by the treatment of dcs with cocktails containing tlr agonists and proinflammatory cytokines, the cells increased the secretion of the th1-promoting cytokine il-12p70. clinically used probiotics were tested f ... | 2011 | 21707779 |
| preservation of acidified cucumbers with a combination of fumaric acid and cinnamaldehyde that target lactic acid bacteria and yeasts. | ôçé the naturally occurring compound, fumaric acid, was evaluated as a potential preservative for the long-term storage of cucumbers. fumaric acid inhibited growth of lactic acid bacteria (lab) in an acidified cucumber juice medium model system resembling conditions that could allow preservation of cucumbers in the presence of sodium benzoate. forty millimolars of fumaric acid were required to inhibit growth of an extremely aciduric lactobacillus plantarum la0445 strain at ph 3.8. half of this c ... | 2011 | 21824132 |
| short communication: characterization of microflora in mexican chihuahua cheese. | this work was performed to identify the bacterial species present in 10 chihuahua cheeses obtained from commercial producers in mexico using 16s rrna gene analysis. as expected, some of the agar media initially used for isolation were not very selective, supporting the growth of several unrelated bacterial species. sequence analysis identified potential pathogens, including escherichia coli and staphylococcus aureus, in all raw milk samples and 2 pasteurized milk samples. streptococcus thermophi ... | 2011 | 21700016 |
| safety and persistence of orally administered human lactobacillus sp. strains in healthy adults. | the aim of the study was to evaluate the safety and persistence of selected lactobacillus strains in the gastrointestinal tract (git) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. in the first phase of the study, nine individuals consumed capsules containing lactobacillus gasseri 177 and e16b7, lactobacillus acidophilus 821-3, lactobacillus paracasei 317 and lactobacillus fermentu ... | 2011 | 21831792 |
| survival of probiotic lactobacilli in the upper gastrointestinal tract using an in-ávitro gastric model of digestion. | the aim of this study was to investigate survival of three commercial probiotic strains (lactobacillus casei subsp. shirota, l. casei subsp. immunitas, lactobacillus acidophilus subsp. johnsonii) in the human upper gastrointestinal (gi) tract using a dynamic gastric model (dgm) of digestion followed by incubation under duodenal conditions. water and milk were used as food matrices and survival was evaluated in both logarithmic and stationary phase. the % of recovery in logarithmic phase ranged f ... | 2011 | 21839386 |
| antifungal activity of 2 lactic acid bacteria of the weissella genus isolated from food. | ôçé in the present study, a total of 116 lactic acid bacteria (lab) strains isolated from mill flour and fermented cassava were screened for their antifungal activity. three strains among 116 were selected for their strongest inhibitory activity against food molds. these 3 strains were lactobacillus plantarum ve56, weissella cibaria fmf4b16, and w. paramesenteroides lc11. the compounds responsible for the antifungal activity were investigated. the strains displayed an inhibitory activity against ... | 2011 | 21729073 |
| sequencing and analysis of three plasmids from lactobacillus casei tistr1341 and development of plasmid-derived escherichia coli-l. casei shuttle vectors. | pyrosequencing followed by conventional pcr and sequencing was used to determine the complete nucleotide sequence of three plasmids (prceid2.9, prceid3.2, and prceid13.9) from the lactobacillus casei strain tistr1341. the plasmid sequences were found to be almost identical, respectively, to those of pla106, pla105, and pla103 from lactobacillus acidophilus strain tk8912, suggesting that these strains may be related. sequence analysis and comparison indicated that prceid2.9 replicates by a rollin ... | 2011 | 21822904 |
| functional characterisation of lp_2714, an eal-domain protein from lactobacillus plantarum. | bioinformatic analysis of lp_2714 from lactobacillus plantarum wcfs1 demonstrates that it encodes an eal-domain protein associated with a membrane targeting signal-sequence. comparison of the predicted primary amino-acid sequence of lp_2714 shows that it lacks critical catalytic residues and heterologous expression has determined that it does not encode a functional phosphodiesterase. we designate lp_2714 as a class-3 eal domain protein probably involved in regulating polysaccharide synthesis on ... | 2011 | 21723265 |
| effects of synbiotic fermentation products on primary chemoprevention in human colon cells. | the consumption of synbiotics, a mixture of probiotics and indigestible food constituents such as dietary fiber, has been reported to reduce colon cancer risk. we investigated the effects of fermented wheat aleurone enriched with the probiotics lactobacillus rhamnosus gg/bifidobacterium animalis supsp. lactis on the gene expression and functional end points related to cellular defence in ht29 and primary human colon cells. aleurone was digested and fermented in vitro with/without probiotics. the ... | 2011 | 21840698 |
| evolution of fermenting microbiota in tarhana produced under controlled technological conditions. | the purpose of this study was to evaluate the evolution of lactic acid bacteria (lab) and yeasts during the fermentation of tarhana produced with some pasteurised ingredients and carried out at 30 and 40-á-¦c. the chemical parameters were those typical for tarhana production. coliform bacteria were not detected during fermentation, while lab and yeasts were in the range 10(7)-10(8)-ácolony forming units (cfu)-ág(-1). plate counts showed an optimal development of both fermenting microbial groups ... | 2011 | 21839387 |
| microencapsulation of lactobacillus plantarum (mtcc 5422) by spray-freeze-drying method and evaluation of survival in simulated gastrointestinal conditions. | spray-drying (sd) and freeze-drying (fd) are widely used methods for microencapsulation of heat-sensitive materials like probiotics for long-term preservation and transport. spray-freeze-drying (sfd) is relatively a new technique that involves spraying a solution into a cold medium and removal of solvent (water) by conventional vacuum fd method. in this study, the sfd microencapsulated lactobacillus plantarum powder (1:1 and 1:1.5 core-to-wall ratios of whey protein) is compared with the microen ... | 2011 | 21827359 |
| lactobacillus plantarum (vr1) isolated from an ayurvedic medicine (kutajarista) ameliorates in vitro cellular damage caused by aeromonas veronii. | abstract: | 2011 | 21707995 |
| impairment of swimming motility by antidiarrheic lactobacillus acidophilus strain lb retards internalization of salmonella enterica serovar typhimurium within human enterocyte-like cells. | we report that the culture and cell-free culture supernatant (cfcs) of l. acidophilus lb have the ability: (i) to delay the appearance of s. typhimurium sl1344-induced mobilization of f-actin; and (ii) subsequently, to retard the cell-entry of s. typhimurium sl1344. time-lapse imaging and western immunoblotting showed that the s. typhimurium sl1344 swimming motility cell-track types were rapidly but temporarily blocked without affecting the expression of flic flagellar propeller protein. we show ... | 2011 | 21825295 |
| global transcriptome response in lactobacillus sakei during growth on ribose. | abstract: | 2011 | 21702908 |
| alterations in microbiota and fermentation products in equine large intestine in response to dietary variation and intestinal disease. | we aimed to determine the effects of variations in dietary composition on equine gut microbiota and their fermentation products, and proposed that dietary modifications profoundly affect microbial ecosystems and their metabolites. bacterial communities within the large intestine of three groups of horses were compared using oligonucleotide-rna hybridisation methodology. each group consisting of six horses was maintained on (1) a grass-only diet, (2) a concentrate diet (i.e. supplemented with hyd ... | 2011 | 21816118 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin a in lactic acid bacteria. | replacement of the leader sequence (ls) of the bacteriocin enterocin a (ls(enta)) by the signal peptides (sp) of the protein usp45 (sp(usp45)), and the bacteriocins enterocin p (sp(entp)), and hiracin jm79 (sp(hirjm79)) permits the production, secretion, and functional expression of enta by different lactic acid bacteria (lab). chimeric genes encoding the sp(usp45), the sp(entp), and the sp(hirjm79) fused to mature enta plus the enta immunity genes (enta+entia) were cloned into the expression ve ... | 2011 | 21839785 |
| nod dendritic cells stimulated with lactobacilli preferentially produce il-10 versus il-12 and decrease diabetes incidence. | dendritic cells (dcs) from nod mice produced high levels of il-12 that induce ifn╬│-producing t cells involved in diabetes development. we propose to utilize the microorganism ability to induce tolerogenic dcs to abrogate the proinflammatory process and prevent diabetes development. nod dcs were stimulated with lactobacilli (nonpathogenic bacteria targeting tlr2) or lipoteichoic acid (lta) from staphylococcus aureus (tlr2 agonist). lta-treated dcs produced much more il-12 than il-10 and accelera ... | 2011 | 21716731 |
| in vitro evaluation of gastrointestinal survival of lactobacillus amylovorus dsm 16698 alone and combined with galactooligosaccharides, milk and/or bifidobacterium animalis subsp. lactis bb-12. | probiotic properties of lactobacillus amylovorus dsm 16698 were previously demonstrated in piglets. here, its potential as a human probiotic was studied in vitro, using the tim-1 system, which is fully validated to simulate the human upper gastrointestinal tract. to evaluate the effect of the food matrix composition on the survival of l. amylovorus dsm 16698 in tim-1, the microorganism was inoculated alone or with prebiotic galactooligosaccharides (gos), partially skimmed milk (psm) and/or comme ... | 2011 | 21741105 |
| an antibacterial coating based on a polymer/sol-gel hybrid matrix loaded with silver nanoparticles. | abstract: in this work a novel antibacterial surface composed of an organic-inorganic hybrid matrix of tetraorthosilicate and a polyelectrolyte is presented. a precursor solution of tetraethoxysilane (teos) and poly(acrylic acid sodium salt) (paa) was prepared and subsequently thin films were fabricated by the dip-coating technique using glass slides as substrates. this hybrid matrix coating is further loaded with silver nanoparticles using an in situ synthesis route. the morphology and composit ... | 2011 | 21711825 |
| effect of functional yogurt ny-yp901 in improving the trait of metabolic syndrome. | background/objectives:this study was aimed to assess the beneficial effects on metabolic syndrome of functional yogurt ny-yp901 (namyang dairy product co. ltd and nutra r&bt inc., seoul, korea) supplemented with mixture of streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium infantis and extra-ingredients containing bifidobacterium breve (cbg-c2), enterococcus faecalis fk-23, fibersol-2 and so on.subjects/methods:this study was designed as an 8-week randomized, double-blind, pl ... | 2011 | 21697819 |
| lactobacillus s-layer protein inhibition of salmonella-induced reorganization of the cytoskeleton and activation of mapk signalling pathways in caco-2 cells. | surface layer (s-layer) proteins are crystalline arrays of proteinaceous subunits that are present as the outermost component of the cell wall in several lactobacillus species. the s-layer proteins have been shown to play a role in the antimicrobial activity of certain lactobacilli. however, it is not fully understood how the s-layer proteins exert this biological function. the aim of this study was to test the hypothesis that lactobacillus acidophilus s-layer proteins antagonize salmonella typh ... | 2011 | 21700664 |
| prevention of vaginal shiv transmission in macaques by a live recombinant lactobacillus. | most human immunodeficiency virus (hiv) transmissions in women occur through the cervicovaginal mucosa, which is coated by a bacterial biofilm including lactobacillus. this commensal bacterium has a role in maintaining a healthy mucosa and can be genetically engineered to produce antiviral peptides. here, we report a 63% reduction in transmission of a chimeric simian/hiv (shiv(sf162p3)) after repeated vaginal challenges of macaques treated with lactobacillus jensenii expressing the hiv-1 entry i ... | 2011 | 21734653 |
| in vitro antimicrobial effects of commercially available mouth-wetting agents. | products have been developed to provide palliation for persons with dry mouth. in addition to mouth-wetting agents, some products incorporate antimicrobial constituents with the goal of improving oral microbial defenses. the aim of this in vitro study was to investigate the potential antimicrobial and antifungal effects of two commercially available saliva substitutes on streptococcus mutans, lactobacillus acidophilus, and candida albicans by using the agar-well diffusion method. antimicrobial a ... | 2011 | 21729120 |
| whole-genome shotgun sequencing of an indian-origin lactobacillus helveticus strain, mtcc 5463, with probiotic potential. | lactobacillus helveticus mtcc 5463 was isolated from a vaginal swab from a healthy adult female. the strain exhibited potential probiotic properties, with their beneficial role in the gastrointestinal tract and their ability to reduce cholesterol and stimulate immunity. we sequenced the whole genome and compared it with the published genome sequence of lactobacillus helveticus dpc4571. | 2011 | 21705605 |
| the importance of inter-species cell-cell co-aggregation between lactobacillus plantarum ml11-11 and saccharomyces cerevisiae by4741 in mixed-species biofilm formation. | cells of lactobacillus plantarum ml11-11, an isolate from fukuyama pot vinegar, and the yeast saccharomyces cerevisiae formed significant mixed-species biofilms with concurrent inter-species co-aggregation. the co-aggregation did not occur with heated or proteinase k-treated ml11-11 cells, or in the presence of d-mannose, suggesting that surface proteins of ml11-11 and mannose-containing surface substance(s) of yeast were the predominant contributing factors. sugar fatty acid ester inhibited mix ... | 2011 | 21821955 |
| antagonistic effect of lactobacillus strains against gas-producing coliforms isolated from colicky infants. | infantile colic is a common disturb within the first 3 months of life, nevertheless the pathogenesis is incompletely understood and treatment remains an open issue. intestinal gas production is thought to be one of the causes of abdominal discomfort in infants suffering from colic. however, data about the role of the amount of gas produced by infants' colonic microbiota and the correlation with the onset of colic symptoms are scanty. the benefit of supplementation with lactobacilli been recently ... | 2011 | 21718486 |
| release of bacteriocins from nanofibers prepared with combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo). | plantaricin 423, produced by lactobacillus plantarum, and bacteriocin st4sa produced by enterococcus mundtii, were electrospun into nanofibers prepared from different combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo) dissolved in n,n-dimethylformamide (dmf). both peptides were released from the nanofibers with a high initial burst and retained 88% of their original antimicrobial activity at 37 -¦c. nanofibers have the potential to serve as carrier matrix for bacteriocins a ... | 2011 | 21731433 |
| characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products. | the present work was aimed at characterizing 12 strains of lactic acid bacteria (lab) to obtain improved potential starter or probiotic cultures that could be used for making dairy products from ewe's milk and cow's milk. eight strains with antimicrobial properties, isolated from ewe's milk and from cheese made from ewe's and/or cow's milk, were studied. they were identified as enterococcus faecalis (five strains), lactococcus lactis subsp. cremoris, leuconostoc mesenteroides, and lactobacillus ... | 2011 | 21819671 |
| suppression subtractive hybridization identifies bacterial genomic regions that are possibly involved in hbd-2 regulation by enterocytes. | scope: human +¦-defensin 2 (hbd-2) is an inducible antimicrobial peptide synthesized by the epithelium to counteract bacterial adherence and invasion. it has been suggested that probiotic bacteria sustain gut barrier function via induction of defensins. the goals of this study were (i) to evaluate the potential immunomodulatory effects of 11 different lactobacillus fermentum strains isolated from kimere, an african fermented pearl millet (pennisetum glaucum) dough, on the hbd-2 secretion by huma ... | 2011 | 21710560 |
| isolation and identification of lactic acid bacteria from tarag in eastern inner mongolia of china by 16s rrna sequences and dgge analysis. | tarag is a characteristic fermented dairy product with rich microflora (especially lactic acid bacteria), developed by the people of mongolian nationality in inner mongolia of china and mongolia throughout history. one hundred and ninety-eight samples of tarag were collected from scattered households in eastern inner mongolia, and total of 790 isolates of lactic acid bacteria (lab) were isolated by traditional pure culture method. to identify these isolates and analyze their biodiversity, 16s rr ... | 2011 | 21689912 |
| organism diversity between women with and without bacterial vaginosis as determined by polymerase chain reaction denaturing gradient gel electrophoresis and 16s rrna gene sequence. | aims:ôçé the aim of this study was to characterize the different structures of microbial communities between 20 healthy women and 17 bacterial vaginosis (bv)-positive women of reproductive age using denaturing gradient gel electrophoresis (dgge). material and methods:ôçé vaginal samples from 17ôçâbv-positive and 20ôçâbv-negative women were subjected to dna extraction, and amplified with eubacterial 16s rrna gene-specific primers via polymerase chain reaction. the polymerase chain reaction produc ... | 2011 | 21676075 |
| [study composition of lactoflora of the large intestine in patients with food allergy and irritable bowel syndrome]. | patients of different ages with various forms of food allergy and also with the irritable bowel syndrome with locks are studied. the quantitative levels of bifido- and lactoflora of large intestine, the qualitative characteristics of lactoflora population, including specific gravity of individual representatives in the sum of the isolates, are investigated, species composition is identified. shown that food allergy, irrespective of the disease manifestation form induces the balance disturbance o ... | 2011 | 21692345 |
| identification of the binding modes of n-phenylphthalimides inhibiting bacterial thymidylate synthase through x-ray crystallography screening. | to identify specific bacterial thymidylate synthase (ts) inhibitors, we exploited phenolphthalein (pth), which inhibits both bacterial and human enzymes. the x-ray crystal structure of lactobacillus casei ts (lcts) that binds pth showed multiple binding modes of the inhibitor, which prevented a classical structure-based drug design approach. to overcome this issue, we synthesized two phthalimidic libraries that were tested against ts enzymes and then we performed x-ray crystallographic screening ... | 2011 | 21696158 |
| cariogenic microorganisms and oral conditions in asthmatic children. | this study evaluated the caries risk of asthmatic patients on the basis of mutans streptococci (ms) and lactobacilli levels in saliva samples as well as the index of oral hygiene and dental caries (dmft index). the study population was composed of 80 asthmatic children, aged 3-15 years, who use specific medication, and 80 matched, healthy control children. the parents were interviewed about oral health-related factors. the world health organization criteria were used for dental examinations. the ... | 2011 | 21822017 |
| lactobacillus nasuensis sp. nov., a lactic acid bacterium isolated from silage, and emended description of the genus lactobacillus. | two strains of lactic acid bacteria isolated from silage prepared with sudangrass (sorghum sudanense (piper) stapf.) were characterized taxonomically. they were gram-positive, catalase-negative and facultative anaerobic rods that did not produce gas from glucose. these strains exhibited intragroup dna-dna hybridization values of over 93.5% and shared the same phenotypic characteristics, indicating that they comprised a single species. the g+c contents of the dna for the strains were 58.5-59.2 mo ... | 2011 | 21724957 |
| complete genome sequence of lactobacillus kefiranofaciens zw3. | lactobacillus kefiranofaciens zw3 was isolated in tibet, china, from kefir grain, a traditional dairy product that is known to provide many health benefits to humans. here, we present the genome features of l. kefiranofaciens zw3 and the identification of a gene cluster related to the synthesis of exopolysaccharide, an important constituent of the tibetan kefir. | 2011 | 21705607 |
| aflatoxin accumulation in whole crop maize silage as a result of aerobic exposure. | most of the maize silage stored in horizontal silos is exposed to air and can be spoiled by fungi. potentially toxigenic fungi have been found in maize silage, and about 300 mycotoxins have been detected. among these mycotoxins, the most harmful for feed and food safety are aflatoxins. the aim of the study was to set up a specific method to detect aflatoxins in maize silage, and to investigate whether aflatoxin contamination in maize silage depends on the level of field contamination of the crop ... | 2011 | 21710665 |
| effects of hydrogen peroxide-containing bleaching on cariogenic bacteria and plaque accumulation. | to evaluate the effects of a commercial bleaching agent containing 36% hydrogen peroxide on the clinical plaque index and streptococcus mutans and lactobacilli counts in dental plaque and saliva in vivo. | 2011 | 21734947 |
| correlation of rrna gene amplicon pyrosequencing and bacterial culture for microbial compositional analysis of faecal samples from elderly irish subjects. | the aim of this investigation was to establish the degree of correlation between measurements from culture-dependent microbiological techniques and from next generation sequencing technologies. | 2011 | 21718396 |
| sequence analysis of the lactobacillus temperate phage sha1. | bacteriophage sha1, a newly isolated temperate phage from a mitomycin-c-induced lysate of lactobacillus plantarum isolated from kimchi, has an isometric head (58-ánm-á+ù-á60-ánm) and a long tail (259-ánm-á+ù-á11-ánm). the double-strand dna genome of the phage sha1 was 41,726 base pairs (bp) long, with a g+c content of 40.61%. bioinformatic analysis of sha1 shows that this phage contains 58 putative open reading frames (orfs). sha1 can be classified as a member of the large family siphoviridae by ... | 2011 | 21701917 |
| lactobacilli secreting a tumor antigen and il15 activates neutrophils and dendritic cells and generates cytotoxic t lymphocytes against cancer cells. | lactobacillus rhamnosus gg (lgg) has been used to successfully induce tumor regression in an orthotopic model of bladder cancer. increased infiltration of neutrophils and macrophages into the tumor mass was observed after therapy. this study evaluates the potential of lgg to induce a directed anti-tumor response. lactobacilli were modified to secrete the prostate specific antigen (psa) or il15 and psa (il-15-psa). neutrophils and dc were exposed to lgg for 2h as in clinical therapy for bladder c ... | 2011 | 21723537 |
| short-term consumption of probiotic lactobacilli has no effect on acid production of supragingival plaque. | acidogenicity and the levels of mutans streptococci (ms) in dental plaque after the use of lactobacillus rhamnosus gg (lgg) and lactobacillus reuteri were determined. the study had a randomised, double-blind, crossover design. thirteen volunteers used tablets containing lgg or a combination of l. reuteri sd2112 and pta 5289 for 2-áweeks. at baseline and at the end of each tablet period, all available supragingival plaque was collected. lactic acid production was determined from a fixed volume (8 ... | 2011 | 21732090 |
| the role of components of bifidobacterium and lactobacillus in pathogenesis and serologic diagnosis of autoimmune thyroid diseases. | during recent years, researchers have been focusing on the concept of an infectious etiology of autoimmune diseases. the most discussed theory is molecular mimicry, i.e. the emergence of autoreactive clones of t- and b-lymphocytes as a result of cross-immune response to homologous bacterial or viral antigen. information on the role of probiotic microorganisms (pm) in the molecular mechanisms of autoimmune thyroid diseases (atd) is limited. using proteins and immunogenic peptides databanks and re ... | 2011 | 21831795 |
| anti-infective properties of lactobacillus fermentum against staphylococcus aureus and pseudomonas aeruginosa. | surgical wounds and implant-associated staphylococcus aureus and pseudomonas aeruginosa infections are often difficult to treat because of limited susceptibility of several of these strains to conventional antibiotics. as a result, there is a constant need for new alternative drugs. the aim of this study was to investigate the antimicrobial properties of lactobacillus fermentum, a probiotic bacterium, which we have isolated from colonic biopsies. the inhibition of s. aureus and p. aeruginosa gro ... | 2011 | 21701187 |
| diversity of bacteria and yeast in the naturally fermented cotton seed and rice beverage produced by brazilian amerindians. | microorganisms associated with the fermentation of cotton seed and rice were studied using a combination of culture-dependent and -independent methods. samples of the cotton seed and rice beverage were collected every 8-áh during the fermentation process for analysis of the microbiota present over 48-áh. the lactic acid bacteria (lab) population reached values of approximately 8.0-álog-ácfu/ml. a total of 162 bacteria and 81 yeast isolates were identified using polyphasic methods. lab (lactobaci ... | 2011 | 21839389 |
| effects of two lactobacillus strains on lipid metabolism and intestinal microflora in rats fed a high-cholesterol diet. | the hypocholesterolemic effects of lactic acid bacteria (lab) have now become an area of great interest and controversy for many scientists. in this study, we evaluated the effects of lactobacillus plantarum 9-41-a and lactobacillus fermentum m1-16 on body weight, lipid metabolism and intestinal microflora of rats fed a high-cholesterol diet. | 2011 | 21722398 |
| genome sequence of lactobacillus ruminis spm0211, isolated from a fecal sample from a healthy korean. | lactobacillus ruminis spm0211 is a potential probiotic strain that shows antimicrobial activity against emerging pathogens. here we present the draft genomic sequence of l. ruminis spm0211, isolated from a fecal sample from a healthy korean and describe both the common and unique features of this strain. | 2011 | 21742873 |
| [survival of probiotic microorganisms in the conditions in vitro imitating the process of human digestion]. | assessment of survival bifidobacteria and lactobacteria under the conditions in vitro, simulating digestion in human stomach and intestine, and study of survival probiotic and indigenous microorganisms in co-cultivation on solid nutrient medium. | 2011 | 21695946 |
| screening of representative cider yeasts and bacteria for volatile phenol-production ability. | representative cider microorganisms (47 yeast strains and 16 bacterial strains) were studied for their ability to produce volatile phenols in a synthetic medium simulating cider conditions and supplemented with the necessary precursors. the various strains were tested for cinnamoyl esterase activity and only lactobacillus collinoides were able to hydrolyse chlorogenic acid. phenolic acid decarboxylase (pad) activities were observed for 6 yeasts and 4 bacterial species allowing them to produce vi ... | 2011 | 21839372 |
| in situ analysis of multispecies biofilm formation on customized titanium surfaces. | many studies to identify surfaces that enhance the incorporation of dental implants into bone and soft-tissue have been undertaken previously. however, to succeed in the clinical situation, an implant surface must not support development of microbial biofilms with a pathogenic potential. as a first step in investigating this, we used two-species and three-species biofilm models with 16s ribosomal rna fluorescence in situ hybridization and confocal laser scanning microscopy to examine the effect ... | 2011 | 21729245 |
| lactobacillus brevis cd2 lozenges reduce radiation- and chemotherapy-induced mucositis in patients with head and neck cancer: a randomized double-blind placebo-controlled study. | background: oral mucositis is a frequent and serious complication in patients receiving chemo-radiotherapy for head and neck squamous cell carcinoma. this study evaluated the effects of administering lactobacillus brevis cd2 lozenges on the incidence and severity of mucositis and tolerance to chemo-radiotherapy. methods: two hundred patients suitable for chemo-radiotherapy were enrolled in a randomised, double-blind study to receive daily treatment with lozenges containing either l. brevis cd2 o ... | 2011 | 21741230 |
| molecular characterization of fecal microbiota in patients with viral diarrhea. | the study provides molecular analyses of fecal microbiota of diarrhea patients infected with four different types of viruses. fecal specimens from 52 patients with viral diarrhea (13 each of adenovirus, norovirus, rotavirus, and astrovirus) and six healthy individuals were collected and etiological viral agent was confirmed by enzyme immunoassay and specific pcr. to assess the changes in microbial diversity in patients with viral diarrhea, dna from stool were extracted and characterized by pcr-d ... | 2011 | 21739252 |
| effects of genetically modified t2a-1 rice on faecal microflora of rats during 90 day supplementation. | background: many animal studies have been performed on products with the bacillus thuringiensis insecticidal toxin-encoding gene (bt products), but less have focused on its effects on intestinal microflora owing to difficulties in culturing. this 90 day study was designed to assess unintended effects of genetically modified t2a-1 rice (gmr) on selected intestinal bacteria (lactobacillus group, bifidobacterium genus, escherichia coli subgroup, enterococcus genus and clostridium perfringens) of ra ... | 2011 | 21520451 |
| effect of the continuous intake of probiotic-fermented milk containing lactobacillus casei strain shirota on fever in a mass outbreak of norovirus gastroenteritis and the faecal microflora in a health service facility for the aged. | for conducting effective risk management in long-stay elderly people at a health service facility, we performed an open case-controlled study to evaluate the effect of the intake of probiotic-fermented milk containing lactobacillus casei strain shirota (lcs-fermented milk) on norovirus gastroenteritis occurring in the winter season during the intake period. a total of seventy-seven elderly people (mean age 84 years) were enrolled in the study. during a 1-month period, there was no significant di ... | 2011 | 21521545 |
| modification of selected bacteria and markers of protein fermentation in the distal gastrointestinal tract of pigs upon consumption of chitosan is accompanied by heightened manure odor emissions. | previous studies have indicated that reducing dietary cp may improve n utilization and effectively diminish manure ammonia emissions; however, the response of manure odor emissions to such dietary modifications has been inconsistent. the objective of the current experiment was to induce decreased lactobacilli (dl) numbers in the distal gastrointestinal tract (dgit; cecum + colon) of finishing pigs offered both high- and low-cp diets through consumption of chitosan, and examine the influence of t ... | 2011 | 21521815 |
| use of gases to improve survival of bifidobacterium bifidum by modifying redox potential in fermented milk. | the aim of this work was to study the effect of the oxidoreduction potential, modified using gas, on the growth and survival of a probiotic strain, bifidobacterium bifidum, and 2 yogurt strains, streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus. three fermented milks were manufactured with an initial oxidoreduction potential value adjusted to +440mv (control milk), +350mv (milk gassed with n(2)), and -300mv [milk gassed with n(2) plus 4% (vol/vol) h(2) (n(2)-h(2))]. acidif ... | 2011 | 21524508 |
| survival of probiotic adjunct cultures in cheese and challenges in their enumeration using selective media. | various selective media for enumerating probiotic and cheese cultures were screened, with 6 media then used to study survival of probiotic bacteria in full-fat and low-fat cheddar cheese. commercial strains of lactobacillus acidophilus, lactobacillus casei, lactobacillus paracasei, or bifidobacterium lactis were added as probiotic adjuncts. the selective media, designed to promote growth of certain lactic acid bacteria (lab) over others or to differentiate between lab, were used to detect indivi ... | 2011 | 21524512 |
| influence of ensiling time and inoculation on alteration of the starch-protein matrix in high-moisture corn. | the fates of hydrophobic zein proteins, which encapsulate corn starch to create vitreous endosperm, have not been investigated in high-moisture corn (hmc). to assess influences of ensiling time and inoculation on zein proteins in hmc, quadruplicate samples of 2 random corn hybrids (a and b), containing 25.7 and 29.3% moisture, were ground, inoculated with (i) or without 600,000 cfu/g of lactobacillus buchneri 40788 (lallemand animal nutrition, milwaukee, wi), and ensiled for 0, 15, 30, 60, 120, ... | 2011 | 21524538 |
| assessment of microbial contamination of toothbrush head: an in vitro study. | background: the most common oral hygiene aid used to improve the oral health of an individual is toothbrush and it is often neglected to maintain in a proper aseptic condition, which could probably result in contamination by various micro-organisms. aims: 1) to assess the microbial contamination of the hard deposit on the toothbrush head, between the bristle tufts, after 1 and 3 months of use. 2) to assess the microbial contamination of the hard deposit on the toothbrush head, between the bristl ... | 2011 | 21525668 |
| antimicrobial effectiveness of different preparations of calcium hydroxide. | background: intracanal medicaments have an antibacterial effect on the root canal flora. the effectiveness of such vehicles has to be tested and substantiated against the normal bacterial flora. the various vehicles that were tested for their effectiveness were commercially prepared calcium hydroxide (metapex), saline, glycerine, cmcp, and rexidine-m gel. the bacterial cultures tested for sensitivity were aerobes (staphylococcus aureus, streptococcus viridans, streptococcus pyogens, enterococcus ... | 2011 | 21525680 |
| the anti-biofouling effect of lactobacillus fermentum-derived biosurfactant against streptococcus mutans. | biofouling in the oral cavity often causes serious problems. the ability of streptococcus mutans to synthesize extracellular glucans from sucrose using glucosyltransferases (gtfs) is vital for the initiation and progression of dental caries. recently, it was demonstrated that some biological compounds, such as secondary metabolites of probiotic bacteria, have an anti-biofouling effect. in this study, s. mutans was investigated for the anti-biofouling effect of lactobacillus fermentum (l.f.)-deri ... | 2011 | 21526440 |
| effects of perioperative synbiotic treatment on infectious complications, intestinal integrity, and fecal flora and organic acids in hepatic surgery with or without cirrhosis. | background: the aim of this study was to assess the effect of preoperative and postoperative synbiotic treatment in hepatectomy patients with or without liver cirrhosis. methods: sixty-one patients with hepatic cancer were assigned randomly to receive either oral synbiotics that consisted of bifidobacterium, lactobacillus, and galactooligosaccharides or no synbiotics (control) preoperatively for 14 days and postoperatively for 11 days. infectious complications, intestinal mucosal integrity as me ... | 2011 | 21527594 |
| construction and characterization of lactobacillus pentosus expressing the d antigenic site of the spike protein of transmissible gastroenteritis virus. | this study explored the feasibility of lactobacillus pentosus as a live vehicle to deliver and express antigen. first of all, l. pentosus transformed by electroporation with the plasmids pg611-6d (anchored) and pg612-6d (secretory) based on the xylose operon generated the recombinant strains rlppg611-6d and rlppg612-6d, respectively, expressing the d antigenic site of the spike (s) protein of transmissible gastroenteritis virus (tgev), for intragastric administration in mice. secondly, we collec ... | 2011 | 21529121 |