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testing of viscous anti-hiv microbicides using lactobacillus.the development of topical microbicides for intravaginal use to prevent hiv infection requires that the drugs and formulated products be nontoxic to the endogenous vaginal lactobacillus. in 30min exposure tests we found dapivirine, tenofovir and uc781 (reverse transcriptase inhibitor anti-hiv drugs) as pure drugs or formulated as film or gel products were not deleterious to lactobacillus species; however, psc-rantes (a synthetic ccr5 antagonist) killed 2 strains of lactobacillus jensenii. to dem ...201122226641
effects of hydrolysed saccharomyces cerevisiae yeast and yeast cell wall components on live performance, intestinal histo-morphology and humoral immune response of broilers.1. the effects of enzymatically hydrolysed whole saccharomyces cerevisiae yeast (hy) and the pellets of yeast cell wall (ycw) on production traits, the microbiology and histo-morphology of the small intestine, and humoral immune responses against newcastle disease virus (ndv), of ross 308 broilers were investigated. 2. the control group received a maize-soyabean meal based basal diet for 42 days. in the treated groups the basal diet was supplemented with 1 g/kg of hy and ycw. there were 8 repl ...201122221235
effect of tween series on growth and cis-9, trans-11 conjugated linoleic acid production of lactobacillus acidophilus f0221 in the presence of bile salts.cis-9, trans-11 conjugated linoleic acid (c9, t11 cla) producing bacteria have attracted much attention as novel probiotics which have shown beneficial effects on host health. however, bile salts are able to inhibit bacterial growth and c9, t11 cla production. for recovering growth and c9, t11 cla production of lactobacillus acidophilus f0221 in the presence of bile salts, tween series (tween 20, tween 40, tween 60 and tween 80) were added in growth culture containing 0.3% oxgall. results showed ...201122272124
evidence for gardnerella vaginalis uptake and internalization by squamous vaginal epithelial cells: implications for the pathogenesis of bacterial vaginosis.bacterial vaginosis (bv), a common condition seen in premenopausal women, is associated with preterm labor, pelvic inflammatory disease, and delivery of low birth weight infants. gardnerella vaginalis is the predominant bacterial species associated with bv, although its exact role in the pathology of bv is unknown. using immunofluorescence, confocal and transmission electron microscopy, we found that vk2 vaginal epithelial cells take up g. vaginalis after exposure to the bacteria. confocal micro ...201122227318
in vitro selection and transferability of antibiotic resistance in the probiotic strain lactobacillus reuteri dsm 17938. 201122233824
[quorum-sensing behavior of lactobacillus paracasei hd1.7].paracin 1.7 was a bacteriocin extracted from lactobacillus paracasei hd1.7. in this study, we investigated quorum-sensing mechanism that regulates the metabolism of paracin 1.7 in strain hd1.7.201122260055
microchip electrophoresis of bacteria using lipid-based liquid crystalline nanoparticles.the aggregation and adhesion of bacterial cells is a serious disadvantage for electrophoretic separations of bacteria. in this study, lipid-based liquid crystalline nanoparticles were used as a pseudostationary phase to minimise the bacterial aggregation and adsorption to the inner walls of microchannels. lactobacillus delbrueckii subsp. bulgaricus, streptococcus thermophilus and lactobacillus rhamnosus were selected as analytes and were separated by microchip electrophoresis (mce) with laser-in ...201121968347
alginate films containing lactobacillus plantarum as wound dressing for prevention of burn infection.the topical application of lactobacilli is receiving attention for the prevention of superficial skin and burn wound infections. we studied the properties of lactobacillus plantarum immobilized in calcium alginate films and investigated the antibacterial activity of these films in a model burn wound in rats. a multiresistant clinical isolate, vim-2-metallo-β-lactamase-producing pseudomonas aeruginosa, was used as the indicator strain. films incorporating l. plantarum at cell concentrations of 10 ...201122000853
antimicrobial activity of an amazon medicinal plant (chancapiedra) (phyllanthus niruri l.) against helicobacter pylori and lactic acid bacteria.the potential of water extracts of the amazon medicinal plant chancapiedra (phyllanthus niruri l.) from ecuador and peru for antimicrobial activity against helicobacter pylori and different strains of lactic acid bacteria such as lactobacillus acidophilus, lactobacillus casei and lactobacillus plantarum was investigated. h. pylori was inhibited by both water extracts in a dose dependent manner, whereas lactic acid bacterial growth was not affected. both extracts contained ellagic acid and hydrox ...201122034238
Effects of microencapsulated Allyl isothiocyanate (AITC) on the extension of the shelf-life of Kimchi.Allyl isothiocyanate (AITC) is a well-recognized antimicrobial agent but, application of AITC to food systems is limited due to its high volatility and strong odor. This study was performed to overcome the volatility of AITC by encapsulation using gum Arabic and chitosan and to investigate the effect of microencapsulated AITC as a natural additive on the shelf-life and quality of Kimchi. AITC loaded microparticles were prepared using gum Arabic and chitosan and were added to Kimchi at various co ...201122104119
Probiotic-derived polyphosphate enhances the epithelial barrier function and maintains intestinal homeostasis through integrin-p38 MAPK pathway.Probiotics exhibit beneficial effects on human health, particularly in the maintenance of intestinal homeostasis in a complex manner notwithstanding the diversity of an intestinal flora between individuals. Thus, it is highly probable that some common molecules secreted by probiotic and/or commensal bacteria contribute to the maintenance of intestinal homeostasis and protect the intestinal epithelium from injurious stimuli. To address this question, we aimed to isolate the cytoprotective compoun ...201121858054
Immunization of chukar partridges against coccidia (Eimeria kofoidi and Eimeria legionensis) with low doses of live oocysts.Experiments were conducted to determine whether chukar partridge (Alectoris chukar) chicks would develop protective immunity after inoculation with coccidia. Young chukar chicks in battery cages inoculated with 100 or more oocysts of Eimeria kofoidi or Eimeria legionensis had significant protection at challenge 4 wk later, as measured by greatly reduced oocyst shedding and improved weight gain as compared with unvaccinated, challenged controls. However, when birds were housed in litter pens and ...201122017029
Coordination chemistry and biological activity of 5'-OH modified quinoline-B12 derivatives.The consequences of structural modifications at the 5'-OH ribofuranotide moiety of quinoline modified B12 derivatives are discussed in regard of the coordination chemistry, the electrochemical properties and the biological behaviour of the compound.201121850334
Daily intake of Lactobacillus casei Shirota increases natural killer cell activity in smokers.Dietary probiotics supplementation exerts beneficial health effects. Since cigarette smoking reduces natural killer (NK) activity, we evaluated the effect of Lactobacillus casei Shirota (LcS) intake on NK cytotoxic activity in male smokers. The double-blind, placebo-controlled, randomised study was conducted on seventy-two healthy Italian blue-collar male smokers randomly divided for daily intake of LcS powder or placebo. Before and after 3 weeks of intake, peripheral blood mononuclear cells wer ...201122142891
In vitro fermentation of sugar beet arabino-oligosaccharides by fecal microbiota obtained from patients with ulcerative colitis to selectively stimulate the growth of Bifidobacterium spp. and Lactobacillus spp.The potential prebiotic properties of arabino-oligosaccharides (AOS) derived from sugar beet pulp was studied using mixed cultures of human fecal bacteria from patients with ulcerative colitis (UC), in remission or with active disease, and in healthy controls. These results were compared to those for fructo-oligosaccharides (FOS), which are known to have a prebiotic effect. Fermentation studies were carried out using a small-scale static batch system, and changes in the fecal microbial communiti ...201121984234
identification of the amidotransferase asnb1 as being responsible for meso-diaminopimelic acid amidation in lactobacillus plantarum peptidoglycan.the peptidoglycan (pg) of lactobacillus plantarum contains amidated meso-diaminopimelic acid (mdap). the functional role of this pg modification has never been characterized in any bacterial species, except for its impact on pg recognition by receptors of the innate immune system. in silico analysis of loci carrying pg biosynthesis genes in the l. plantarum genome revealed the colocalization of the mure gene, which encodes the ligase catalyzing the addition of mdap to udp-n-muramoyl-d-glutamate ...201121949063
synbiotic effect of various prebiotics on in vitro activities of probiotic lactobacilli.in the present study, five lactobacillus strains were evaluated for their viability in presence of different prebiotics viz. inulin, oligofructose, lactulose, raftilose, and honey. the viability of lactobacilli was observed before and after 5 weeks of refrigerated storage. the doubling time varied from 5.2 hrs to 9.6 hrs. the lowest doubling time was for lactobacillus plantarum m5 followed by l. plantarum ch1 with inulin. viability of lactobacilli was greatest with inulin. the growth and viabili ...201121888588
follow-up of probiotic lactobacillus gg effects on allergic sensitization and asthma in infants at risk. 201122107145
inhibition of hiv-1 infection in ex vivo cervical tissue model of human vagina by palmitic acid; implications for a microbicide development.approximately 80% of all new hiv-1 infections are acquired through sexual contact. currently, there is no clinically approved microbicide, indicating a clear and urgent therapeutic need. we recently reported that palmitic acid (pa) is a novel and specific inhibitor of hiv-1 fusion and entry. mechanistically, pa inhibits hiv-1 infection by binding to a novel pocket on the cd4 receptor and blocks efficient gp120-to-cd4 attachment. here, we wanted to assess the ability of pa to inhibit hiv-1 infect ...201121949756
alternative antimicrobial compounds to control potential lactobacillus contamination in bioethanol fermentations.antibiotics are commonly used to control microbial contaminants in yeast-based bioethanol fermentation. given the increase in antibiotic-resistant bacteria, alternative natural antimicrobials were evaluated against the potential contaminant, lactobacillus. the effects of nisin, ϵ-polylysine, chitosan (cs) and lysozyme were screened against 5 lactobacillus strains. a standard broth- microdilution method was used in 96-well plates to assess the minimal inhibitory concentration (mic). l. delbruecki ...201121879832
Immunization with recombinant Lactobacillus casei strains producing K99, K88 fimbrial protein protects mice against enterotoxigenic Escherichia coli.To exploit a safe and effective vaccine for the prevention against K99 or K88 infections of enterotoxigenic Escherichia coli (ETEC), we have developed a mucosal delivery vehicle based on Lactobacillus casei CICC 6105 using poly-?-glutamate synthetase A (PgsA) as an anchoring matrix. To evaluate the immunization effect of the recombinant strains (harboring plasmids pLA-K99-K88-LTB, pLA-K99, and pLA-K88), anti-ETEC K99 or K88 antibody responses, T-cell proliferation, and cytokines by intracellular ...201121856357
Surface proteins from Lactobacillus kefir antagonize in vitro cytotoxic effect of Clostridium difficile toxins.In this work, the ability of S-layer proteins from kefir-isolated Lactobacillus kefir strains to antagonize the cytophatic effects of toxins from Clostridium difficile (TcdA and TcdB) on eukaryotic cells in vitro was tested by cell detachment assay. S-layer proteins from eight different L. kefir strains were able to inhibit the damage induced by C. difficile spent culture supernatant to Vero cells. Besides, same protective effect was observed by F-actin network staining. S-layer proteins from ag ...201122126976
effect of enteral nutrition on dextran sulfate sodium-induced colitis in rats.to study the effect of enteral nutrition (en) on dextran sulfate sodium (dss)-induced colitis in rats.201122118695
antagonistic intestinal microflora produces antimicrobial substance inhibitory to pseudomonas species and other spoilage organisms.chicken intestine harbors a vast number of bacterial strains. in the present study, antimicrobial substance produced by lactic acid bacteria (lab) isolated from the gastrointestinal tract of healthy chicken was detected, characterized, and purified. based on 16s rrna sequencing, the bacteria were identified as lactobacillus plantarum vn. the antimicrobial substance produced by this bacterium was designated vn-1 and exhibited a broad-spectrum of activity against many important pathogenic and spoi ...201121913924
using the good to beat out the bad: probiotics for eliminating vancomycin-resistant enterococci colonization. 201121989278
Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults.Several studies have reported limited or no reduction in serum cholesterol in response to probiotic formulations. Recently, probiotics have shown promise in treating metabolic disease due to improved strain selection and delivery technologies. The aim of the present study was to evaluate the cholesterol-lowering efficacy of a yoghurt formulation containing microencapsulated bile salt hydrolase (BSH)-active Lactobacillus reuteri NCIMB 30242, taken twice per d over 6 weeks, in hypercholesterolaemi ...201122067612
characterisation of the oral, vaginal and rectal lactobacillus flora in healthy pregnant and postmenopausal women.to investigate the hypothesis that the rectum may be an important reservoir for vaginal colonisation by lactobacillus species.201122088236
species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional ecuadorian spontaneous cocoa bean fermentations.traditional fermentations of the local ecuadorian cocoa type nacional, with its fine flavor, are carried out in boxes and on platforms for a short time. a multiphasic approach, encompassing culture-dependent and -independent microbiological analyses of fermenting cocoa pulp-bean samples, metabolite target analyses of both cocoa pulp and beans, and sensory analysis of chocolates produced from the respective fermented dry beans, was applied for the investigation of the influence of these fermentat ...201121926224
Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme.N-deoxyribosyltransferases are essential enzymes in the nucleotide salvage pathway of lactobacilli. They catalyze the exchange between the purine or pyrimidine bases of 2'-deoxyribonucleosides and free pyrimidine or purine bases. In general, N-deoxyribosyltransferases are referred to as cytoplasmic enzymes, although there is no experimental evidence for this subcellular localization. In this work, the subcellular localization of N-deoxyribosyltransferase II (NTD) from Lactobacillus fermentum was ...201122092712
fibers from fruit by-products enhance probiotic viability and fatty acid profile and increase cla content in yoghurts.this study evaluated the effect of the supplementation of total dietary fiber from apple, banana or passion fruit processing by-products on the post-acidification, total titratable acidity, bacteria counts and fatty acid profiles in skim milk yoghurts co-fermented by four different probiotics strains: lactobacillus acidophilus l10 and bifidobacterium animalis subsp. lactis bl04, hn019 and b94. apple and banana fibers increased the probiotic viability during shelf-life. all the fibers were able t ...201122264421
intraoral ph measurement of carious lesions with qpcr of cariogenic bacteria to differentiate caries activity.objectives: a low ph environment is created by cariogenic bacteria. this study was aimed to measure ph of carious lesions intraorally using a micro-ph sensor, and assess predominant acid-producing cariogens by qpcr to differentiate caries activities. methods: 103 dentine lesions classified as active or arrested caries based on the clinical and radiological examinations were collected from patients after intraoral measurement of the lesion surface ph using a micro-ph sensor. quantitative detectio ...201122222970
effect of micronutrient and probiotic fortified yogurt on immune-function of anti-retroviral therapy naive hiv patients.background: micronutrient supplementation has been shown to reduce the progression of hiv but does not have an effect on the intestinal barrier or the intestinal microbiota of hiv patients. studies have suggested that probiotics could potentially complement micronutrients in preserving the immune-function of hiv patients. objective: assess the impact of micronutrient supplemented probiotic yogurt on the immune function of hiv patients. design:we performed a randomized, double blind, controlled t ...201122254084
effect of lactobacilli on paracellular permeability in the gut.paracellular permeability is determined by the complex structures of junctions that are located between the epithelial cells. already in 1996, it was shown that the human probiotic strain lactobacillus plantarum 299v and the rat-originating strain lactobacillus reuteri r2lc could reduce this permeability in a methotrexate-induced colitis model in the rat. subsequently, many animal models and cell culture systems have shown indications that lactobacilli are able to counteract increased paracellul ...201122254077
structure of plantaricin locus of lactobacillus plantarum 8p-a3.lactobacillus plantarum 8p-a3 is a strain which is well known on the russian pharmaceutical market and it is included in several probiotic products. the strain has been widely used since 1973 but the mechanisms of its antibacterial activity were unknown. l. plantarum 8p-a3 expressed high antagonistic activity against the wide range of bacterial pathogens including streptococcus agalactiae, streptococcus pyogenes, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa and klebsiella pneu ...201122146685
probiotic therapy: immunomodulating approach toward urinary tract infection.urinary tract infection (uti) is an extremely common health problem, with an unpredictable history. members of enterobacteriaceae family such as escherichia coli, which are normal inhabitants of human intestines, account for the majority of these uncomplicated infections. rarely, uti can result from virus or fungus. there is a close correlation between loss of the normal genital microbiota, particularly lactobacillus species, and an increased incidence of genital and bladder infections. although ...201121901556
Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons.ABSTRACT:201122047020
Probiotic yogurt consumption may improve gastrointestinal symptoms, productivity, and nutritional intake of people living with human immunodeficiency virus in Mwanza, Tanzania.The gut-associated lymphoid tissue is a major site of human immunodeficiency virus (HIV) activity and significantly influences disease prognosis. Reducing immune activation due to gastroenteritis may thus help slow disease progression. Probiotic microorganisms have considerable immunomodulatory effects at the level of the gut-associated lymphoid tissue. A probiotic yogurt initiative was thus established in Mwanza, Tanzania, to improve gastrointestinal (GI) integrity and reduce the incidence and ...201122153512
Characteristics of spoilage-associated secondary cucumber fermentation.Secondary fermentations during the bulk storage of fermented cucumbers can result in spoilage that causes total loss of the fermented product at an estimated cost of $6,000 to $15,000 per affected tank. Previous research has suggested that such fermentations are the result of microbiological utilization of lactic acid and formation of acetic, butyric and propionic acids. The objectives of this study were to characterize the chemical and environmental conditions associated with secondary cucumber ...201122179234
Opinion paper: Quo vadis - EFSA?In times when human life and quality of life remain threatened by infectious diseases, increasing microbial resistance and a diminished armamentarium of treatments, you might think that the European Commission would urgently seek and encourage alternative approaches. On the contrary, the European Food Safety Authority (EFSA) has created a panel that has ignored good science, over-ridden peer-reviewed medical studies, and seems intent on breaking the back of a validated approach to infectious dis ...201121986356
growth and exopolysaccharide yield of lactobacillus delbrueckii ssp. bulgaricus dsm 20081 in batch and continuous bioreactor experiments at constant ph.some lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (eps) and are therefore highly important for the dairy industry as starter cultures. the aim of this study was to investigate the nutritional requirements for growth and eps production of lactobacillus delbrueckii ssp. bulgaricus dsm 20081. a medium was developed from a semi-defined medium (sdm) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, ...201122074989
Probiotic Bacteria Induce Maturation of Intestinal Claudin 3 Expression and Barrier Function.An immature intestinal epithelial barrier may predispose infants and children to many intestinal inflammatory diseases, such as infectious enteritis, inflammatory bowel disease, and necrotizing enterocolitis. Understanding the factors that regulate gut barrier maturation may yield insight into strategies to prevent these intestinal diseases. The claudin family of tight junction proteins plays an important role in regulating epithelial paracellular permeability. Previous reports demonstrate that ...201122155109
Treatment with probiotics in experimental oral colonization by Candida albicans in murine model (DBA/2).Oral Diseases (2011) The aim of this study is to evaluate the oral colonization by Candida albicans in experimental murine immunosuppressed DBA/2 and treatment with probiotic bacteria. To achieve these objectives, 152 DBA/2-immunosuppressed mice were orally inoculated with a suspension of C. albicans containing 10(8) viable yeast cells, the animals were treated with nystatin or with the probiotics (Lactobacillus acidophilus and Lactobacillus rhamnosus). Evaluations were performed by Candida cou ...201122059932
utilization of different types of dietary fibres by potential probiotics.a better understanding of the functionality of probiotics and dietary fibres with prebiotic activity is required for the development of improved synbiotic preparations. in this study, utilization of β(2-1) fructans, galactooligosaccharides, and plant polysaccharides as prebiotics by lactobacilli, bifidobacteria, and pediococci was investigated. our results demonstrate that prebiotics with linear chains consisting of galactose units are better utilized by probiotics than are those consisting of g ...201121958046
description of a french natural wheat sourdough over 10 consecutive days focussing on the lactobacilli present in the microbiota.sourdoughs are complex ecosystems which are widely used to produce baked goods. this study aimed to provide a dynamic description of an industrial french sourdough ecosystem over 10 consecutive days. the sourdough was obtained from a natural fermentation that has been propagated over several years by the traditional backslopping method. the physico-chemical properties (ph, temperature, total titratable acidity) and the diversity of the lactobacilli among the microbiota were analyzed. a culture-d ...201121935690
Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing.It is important to know the comprehensive microbial communities of fecal pollution sources and receiving water bodies for microbial source tracking. Pyrosequencing targeting the V1-V3 hypervariable regions of the 16S rRNA gene was used to investigate the characteristics of bacterial and Bacteroidales communities in major fecal sources and river waters. Diversity analysis indicated that cow feces had the highest diversities in the bacterial and Bacteroidales group followed by the pig sample, with ...201121887641
Combination of culture-dependent and culture-independent molecular methods for the determination of lactic microbiota in sucuk.In this study, the culture-dependent and culture-independent molecular methods were used for the identification of lactic acid bacteria (LAB) in sucuk a Turkish fermented dry sausage. On the one hand, the PCR-DGGE method targetting the V1 and V3 regions of 16S DNA was applied to DNA that was directly extracted from sucuk samples. On the other hand, rep-PCR fingerprinting was performed for the primary differentiation and grouping of the isolates, and the results were confirmed by sequencing of th ...201122209604
Directed chromosomal integration and expression of the reporter gene gusA3 in Lactobacillus acidophilus NCFM.Lactobacillus acidophilus NCFM is a probiotic microbe that survives passage through the human gastrointestinal tract and interacts with the host epithelium and mucosal immune cells. The potential for L. acidophilus to express antigens at mucosal surfaces has been investigated with various antigens and plasmid expression vectors. Plasmid instability and antibiotic selection complicate the possibility of testing these constructs in human clinical trials. Integrating antigen encoding genes into the ...201121873486
Microbial dynamics during the ripening of a mixed cow and goat milk cheese manufactured using frozen goat milk curd.To overcome the seasonal shortage of goat milk in mixed milk cheese manufacture, pasteurized goat milk curd and high-pressure-treated raw goat milk curd manufactured in the spring were held at -24 °C for 4 mo, thawed, and mixed with fresh cow milk curd for the manufacture of experimental cheeses. Control cheeses were made from a mixture of pasteurized cow and goat milk. The microbiota of experimental and control cheeses was studied using culture-dependent and culture-independent techniques. Bact ...201121943728
Effects of daily consumption of probiotic yoghurt on inflammatory factors in pregnant women: a randomized controlled trial.Previous studies have shown that inflammatory factors increases in pregnancy and is associated with several complications of pregnancy. The aim of this study was to assess effects of daily consumption of probiotic yoghurt on inflammatory factors in pregnant women. In a randomized clinical trial, seventy primigravid (the first pregnancy) and singleton pregnant women aged 18-30 years were assigned to two groups. Subjects consumed daily 200 g probiotic yoghurt containing Lactobacillus acidophilus L ...201121936251
Randomised clinical trial: the synbiotic food supplement Probiotical vs. placebo for acute gastroenteritis in children.Some probiotic strains reduce the duration of acute diarrhoea. As a result of strain and product specificity, each product needs support by clinical data.201121899583
Fate of Escherichia coli O26 in Corn Silage Experimentally Contaminated at Ensiling, at Silo Opening, or after Aerobic Exposure, and Protective Effect of Various Bacterial Inoculants.Shiga toxin-producing Escherichia coli (STEC) strains are responsible for human illness. Ruminants are recognized as a major reservoir of STEC, and animal feeds, such as silages, have been pointed out as a possible vehicle for the spread of STEC. The present study aimed to monitor the fate of pathogenic E. coli O26 strains in corn material experimentally inoculated (10(5) CFU/g) during ensiling, just after silo opening, and after several days of aerobic exposure. The addition of 3 bacterial inoc ...201121984243
Impact of acetaldehyde and pyruvic acid bound sulfur dioxide on wine lactic acid bacteria.Aims: To investigate the impact of acetaldehyde and pyruvic acid bound sulfur dioxide on wine lactic acid bacteria. Methods and Results: Growth studies were performed where Oenococcus oeni, Pediococcus parvulus, P. damnosus, and Lactobacillus hilgardii were inoculated into media containing various concentrations of acetaldehyde or pyruvic acid and an equimolar concentration of SO(2) at pH 3.50 and 3.70. Low concentrations of acetaldehyde and pyruvic acid bound SO(2) were inhibitory to the gr ...201122150460
oral colonization by streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase vi expression in saliva.carbonic anhydrase vi (ca vi), encoded by type a transcripts of the gene car6, is a secretory product of salivary glands and is found in the enamel pellicle. because higher caries prevalence is associated with lower salivary concentrations of ca vi in humans, we tested whether ca vi protects enamel surfaces from caries induced by streptococcus mutans, using car6(-/-) mice, in which salivary ca vi expression is absent. we detected aberrant car6 type a transcripts in car6(-/-) mice, likely targets ...201121945428
investigation of biomarkers of bile tolerance in lactobacillus casei using comparative proteomics.the identification of cell determinants involved in probiotic features is a challenge in current probiotic research. in this work, markers of bile tolerance in lactobacillus casei were investigated using comparative proteomics. six l. casei strains were classified on the basis of their ability to grow in the presence of bile salts in vitro. constitutive differences between whole cell proteomes of the most tolerant strain (l. casei rosell-215), the most sensitive one (l. casei atcc 334), and a mo ...201122040141
food-borne bacteremic illnesses in febrile neutropenic children.bacteremia following febrile neutropenia is a serious complication in children with malignancies. preventive measures are currently targeted at antimicrobial prophylaxis, amelioration of drug-induced neutropenia, and nosocomial spread of pathogens, with little attention to community-acquired infections. a retrospective study was conducted at a pediatric oncology center during a 3-year period to identify probable cases of food-borne infections with bacteremia. twenty-one bacteremic illnesses affe ...201122184532
Immunoregulatory potential of exopolysaccharide from Lactobacillus rhamnosus KL37. Effects on the production of inflammatory mediators by mouse macrophages.The ability to produce exopolysaccharides (EPS) is widespread among lactobacilli including Lactobacillus rhamnosus, the commonly used probiotic bacteria. Exopolysaccharides are a major component of the bacterial biofilm with a well-documented impact on adherence of bacteria to host cells. However, their immunoregulatory properties are unknown. The aim of this study was to examine the immunostimulatory potential of EPS derived from L. rhamnosus KL37. We investigated the effect of EPS on the produ ...201121950581
Readers prefer probiotic pills to probiotic yogurt. 201122022756
In vitro testing of commercial and potential probiotic lactic acid bacteria.Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such as transit tolerance in the upper human gastrointestinal tract, adhesion capacity to human intestinal cell lines and effect on epithelial barrier function. The selected bacteria include strains of Lac ...201122177712
Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage.Aims: Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. Methods and Results: Direct-cut and wilted guinea grass silage was prepared with and without molasses. A high acetic acid level was observed during the fermentation of direct-cut silage, and long storage inc ...201122150390
Pretreatment with the probiotic VSL#3 delays transition from inflammation to dysplasia in a rat model of colitis-associated cancer.Evidence supports involvement of microflora in the transition of chronic inflammation to neoplasia. We investigated the protective efficacy of the probiotic VSL#3 in a model of colitis-associated colorectal cancer. Chronic colitis was induced in Sprague-Dawley rats by administration of trinitrobenzene sulfonic acid (TNBS), followed 6 wk later by systemic reactivation. To induce colitis-associated dysplasia and cancer, the animals received TNBS (intravenously) twice a week for 10 wk. One group re ...201121903764
bacterial vaginosis: what is physiological in vaginal bacteriology? an update and opinion.bacterial vaginosis (bv) was originally described as a sexually transmitted infection caused by a single microbe. the prevailing concept describes bv as a polymicrobial coitus-associated disease of uncertain origin. in this overview the natural history of bv as a monobacterial and polymicrobial entity is examined with respect to the physiological concept of bv in relation to sexual behavior before and after introduction of the hormonal contraceptive pill. bacterial vaginosis, characterized by lo ...201121916857
Characterization of Lactobacillus isolates from fermented olives and their bacteriocin gene profiles.Near one hundred isolates of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum from table olives were studied. Strains were genotyped by rep-PCR. Although the technique failed to differentiate some isolates at the species level, it proved a robust and easy procedure that could be useful for distinguishing between related strains of L. paraplantarum, L. pentosus and L. plantarum from a large pool of unrelated strains of these species. A PCR-based screening revealed t ...201121925038
Peptidoglycan recognition protein 3 (PglyRP3) has an anti-inflammatory role in intestinal epithelial cells.Intestinal epithelial cells produce cytokines in response to bacterial peptidoglycan (PGN), which is detected by several classes of pattern-recognition receptors (PRRs) as peptidoglycan recognition proteins (PGlyRPs), Toll-like receptor 2 (TLR2) and NOD receptors. All types of PGlyRPs recognize bacterial peptidoglycan and function in antibacterial innate immunity. In this study, we investigated the role of PGlyRP3 in the response of intestinal epithelial cells (Caco-2) to PGN from pathogenic (St ...201122099350
Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting.BACKGROUND: Four repetitive element sequence-based polymerase chain reaction (rep-PCR) methods, namely repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), polytrinucleotide (GTG)(5) -PCR and BOX-PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the com ...201121919004
In vitro evaluation of Lactobacillus gasseri strains of infant origin on adhesion and aggregation of specific pathogens.Numerous Lactobacillus species are members of the normal healthy human intestinal microbiota, and members of the Lactobacillus family predominate among the current marketed probiotic strains. Most of the current commercial probiotic strains have not been selected for specific applications but rather have been chosen based on their technological properties. Often the ability of such strains to temporarily colonize the gastrointestinal tract may be lacking, and the interactions with intestinal mic ...201121902917
lactobacillus reuteri strains reduce incidence and severity of experimental necrotizing enterocolitis via modulation of tlr4 and nfκb signaling in the intestine.necrotizing enterocolitis (nec) is the leading gastrointestinal cause of mortality and morbidity in the premature infant. premature infants have a delay in intestinal colonization by commensal bacteria and colonization with potentially pathogenic organisms. lactobacillus reuteri (lr) is a probiotic that inhibits enteric infections, modulates the immune system, and may be beneficial to prevent nec. in previous studies, lr strains dsm 17938 and atcc pta 4659 differentially modulated inflammation i ...201122207578
oral administration of lactobacillus plantarum k68 ameliorates dss-induced ulcerative colitis in balb/c mice via the anti-inflammatory and immunomodulatory activities.many different kinds of fermented food are consumed daily in taiwan, such as stinky tofu, suan-tsai, and fu-tsai. we have previously reported the diversity of lactic acid bacteria (lab) at different stages of fermentation in the production of suan-tsai and fu-tsai. in this study, the anti-inflammatory and immunomodulatory activities of lactobacillus plantarum k68 (k68) isolated from fu-tsai were evaluated. k68 significantly inhibited the production of tumor necrosis factor-α (tnf-α) and prostagl ...201121996541
supramolecular stabilization of acid tolerant l-arabinose isomerase from lactobacillus sakei.l-arabinose isomerase stability is a crucial criterion for the industrial application of this biocatalyst. noria and noriapg are capable of increasing the l-arabinose isomerase stability not only at high temperatures but also at low ph. such results highlight, for the first time, the use of the noria series of molecules for protein stabilization and activation.201122012067
In vitro fermentation by human gut bacteria of proteolytically digested caseinomacropeptide nonenzymatically glycosylated with prebiotic carbohydrates.The in vitro fermentation selectivity of hydrolyzed caseinomacropeptide (CMP) glycosylated, via Maillard reaction (MR), with lactulose, galacto-oligosaccharides from lactose (GOSLa), and galacto-oligosaccharides from lactulose (GOSLu) was evaluated, using pH-controlled small-scale batch cultures at 37 °C under anaerobic conditions with human feces. After 10 and 24 h of fermentation, neoglyconjugates exerted a bifidogenic activity, similar to those of the corresponding prebiotic carbohydrates. No ...201122004447
Transit time affects the community stability of lactobacillus and bifidobacterium species in an in vitro model of human colonic microbiotia.Retention time, which is analogous to transit time, is an index for bacterial stability in the intestine. Its consideration is of particular importance to optimize the delivery of probiotic bacteria in order to improve treatment efficacy. This study aims to investigate the effect of retention time on Lactobacilli and Bifidobacteria stability using an established in vitro human colon model. Three retention times were used: 72, 96, and 144 h. The effect of retention time on cell viability of diffe ...201122066794
Lysate of Probiotic Lactobacillus casei DN-114 001 Ameliorates Colitis by Strengthening the Gut Barrier Function and Changing the Gut Microenvironment.Probiotic bacteria can be used for the prevention and treatment of human inflammatory diseases including inflammatory bowel diseases (IBD). However, the nature of active components and exact mechanisms of this beneficial effects have not been fully elucidated. Our aim was to investigate if lysate of probiotic bacterium L. casei DN-114 001 (Lc) could decrease the severity of intestinal inflammation in a murine model of IBD.201122132181
meta-analysis: the effects of lactobacillus rhamnosus gg supplementation for the prevention of healthcare-associated diarrhoea in children.in children, healthcare-associated diarrhoea, in particular, due to rotavirus, may prolong the hospital stay and increase medical costs, prompting interest in effective, low-cost, preventive strategies.201121899584
The effect of supplementation of flax-seed oil on interaction of Lactobacillus plantarum - Biocenol™ LP96 and Escherichia coli O8:K88ab:H9 in the gut of germ-free piglets.We investigated the influence of administration of flax-seed oil on interaction of Lactobacillus plantarum - Biocenol™ LP96 and Escherichia coli O8:K88ab:H9 in the gut of germ-free piglets. When compared to animals supplemented with L. plantarum, the counts of lactobacilli in the jejunal and ileal mucosa and in the intestinal content were significantly higher in LMK group (p<0.0001). Inter-groups comparison of the counts of E. coli K88 adhering to the jejunal and ileal mucosa revealed a signific ...201121864874
A study of consumers' perceptions and prediction of consumption patterns for generic health functional foods.The Korea Food and Drug Administration (KFDA) revised the Health Functional Food Act in 2008 and extended the form of health functional foods to general food types. Therefore, this study was performed to investigate consumers' perceptions of the expanded form of health functional food and to predict consumption patterns. For this study, 1,006 male and female adults aged 19 years and older were selected nationwide by multi-stage stratified random sampling and were surveyed in 1:1 interviews. The ...201121994526
anti-alpha-gal antibody titres remain unaffected by the consumption of fermented milk containing lactobacillus casei in healthy adults.alpha-gal is a glycoconjugate present on cell membranes of non-primate mammals and bacteria, but not in humans, who display anti-gal antibodies (abs) in high titres. probiotics contain bacterial strains which colonize the intestinal tract. in the present study, we investigated whether intake of fermented milk containing lactobacillus casei (fml) affects anti-gal ab titres. serum was drawn from healthy probands (n = 19) for 6 weeks. after the second week, the probands consumed 125 ml of fml per d ...201121970387
In vitro development of resistance to arsenite and chromium-VI in Lactobacilli strains as perspective attenuation of gastrointestinal disorder.Inadvertent intake of inorganic arsenic and chromium through drinking water and food causing their toxic insults is a major health problem. Intestinal bacteria including Lactobacilli play important regulatory roles on intestinal homeostasis, and their loss is known to cause gastrointestinal (GI) disorders. Probiotic Lactobacilli resistance to arsenite and chromium-VI could be an importantfactorfor the perspective attenuation of Gl-disorders caused by these toxic metals/metalloid. In the present ...201122167945
Probiotic properties of Weissella strains isolated from human faeces.Three Weissellaconfusa and five Weissellacibaria strains were previously isolated from human faeces and their potential as probiotics was examined in this work. Resistance to low pHs (pH 2.0 and 3.0) and 0.3% bile salt were examined. Enzyme activities, susceptibilities to heat treatment and various antibiotics, and adhesion capacities to Caco-2 cells were also examined. All Weissella strains were killed when exposed to pH 2.0 for 2 h but survived at pH 3.0 with different survival ratios. W. conf ...201122200451
effect of the inoculation level of lactobacillus acidophilus in probiotic cheese on the physicochemical features and sensory performance compared with commercial cheeses.the complex metabolism of probiotic bacteria requires several technological options to guarantee the functionally of probiotic dairy foods during the shelf life. this research aimed to evaluate the effect of the supplementation of increasing amounts of lactobacillus acidophilus (0, 0.4, or 0.8 g/l of milk) on the physicochemical parameters and sensory acceptance of minas fresh cheese. in addition, the sensory acceptance of probiotic cheeses was assessed using a consumer test and compared with co ...201121943729
classification of swiss cheese starter and adjunct cultures using fourier transform infrared microspectroscopy.the acceptability of swiss cheese largely depends on the flavor profile, and strain variations of cheese cultures will affect the final quality. conventional biochemical methods to identify the cultures at the strain level are time-consuming and expensive, and require skilled labor. our objective was to develop rapid classification methods of starter cultures at the strain level by using a combination of hydrophobic grid membrane filters and fourier transform infrared (ftir) spectroscopy. forty- ...201121854910
successful tigecycline lock therapy in a lactobacillus rhamnosus catheter-related bloodstream infection.introduction: catheter-related bloodstream infections very often involve the premature removal of long-term intravascular devices (ltid). the antibiotic lock therapy (alt) represents a conservative approach to the treatment of uncomplicated infections of tunneled ltid when catheter removal is not a feasible option. case report: we present here the first reported case of tunneled ltid bloodstream infection due to ...201122005933
characterization of a multilayer film activated with lactobacillus curvatus crl705 bacteriocins.background: bacteriocins produced by lactic acid bacteria offer enormous promise for food safety preservation. in this study an active multilayer film obtained by the incorporation of lactocin 705 and lactocin al705, two bacteriocins produced by lactobacillus curvatus crl705 with antimicrobial activity against lactobacillus plantarum crl691 and listeria innocua 7, respectively, was characterized for its potential application in active packaging technology. film activity performance at different ...201122083520
probiotics prevent the development of 1,2-dimethylhydrazine (dmh)-induced colonic tumorigenesis through suppressed colonic mucosa cellular proliferation and increased stimulation of macrophages.probiotics modulate immunity and inhibit colon carcinogenesis in experimental models, but these effects largely depend on the bacterial strain, and the precise mechanisms are not well understood. therefore, we studied the effect of bifidobacterium longum and/or lactobacillus gasseri on the development of 1,2-dimethylhydrazine (dmh)-induced colonic precancerous lesions and tumors in mice while delineating the possible mechanisms involved. the results suggest that dietary consumption of probio ...201122049926
Bacterial DNA from orthopedic implants after routine removal.Bacterial 16S rDNA was monitored and identified from orthopedic metallic implants after routine or septic removal from patients in a German hospital. From March to June 2009, 28 metallic implants, 10 human biopsies, and 6 foam dressings from 28 patients were investigated. After analysis of this first collective, the methods were optimized to enhance sensitivity and to reduce interference with human DNA. Then a second collective consisting of 21 metallic implants from 21 patients was investigated ...201122094566
d-Fagomine lowers postprandial blood glucose and modulates bacterial adhesion.d-Fagomine is an iminosugar originally isolated from seeds of buckwheat (Fagopyrum sculentum Moench), present in the human diet and now available as a pure crystalline product. We tested d-fagomine for activities connected to a reduction in the risk of developing insulin resistance, becoming overweight and suffering from an excess of potentially pathogenic bacteria. The activities were: intestinal sucrase inhibition in vitro (rat mucosa and everted intestine sleeves), modulation of postprandial ...201122017795
Genetic screening of functional properties of lactic Acid bacteria in a fermented pearl millet slurry and in the metagenome of fermented starchy foods.Lactic acid bacteria (LAB) (n = 152) in African pearl millet slurries and in the metagenomes of amylaceous fermented foods were investigated by screening 33 genes involved in probiotic and nutritional functions. All isolates belonged to six species of the genera Pediococcus and Lactobacillus, and Lactobacillus fermentum was the dominant species. We screened the isolates for the abilities to survive passage through the gastrointestinal tract and to synthesize folate and riboflavin. The isolates w ...201122003019
a practical guide to genome-scale metabolic models and their analysis.genome-scale metabolic reconstructions and their analysis with constraint-based modeling techniques have gained enormous momentum. it is a natural next step after sequencing of a genome, as a technique that links top-down systems biology analyses at genome scale with bottom-up systems biology modeling scrutiny. this chapter aims at (systems) biologists that have an interest in, but no extensive knowledge of, applying genome-scale metabolic reconstruction and modeling to their organism. rather th ...201121943912
A comprehensive post-market review of studies on a probiotic product containing Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011.The probiotic preparation Lacidofil® has been commercially available in Europe, Asia and North America since 1995. This product is a combination of two strains, Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011. The strains have been evaluated for safety, identity and mechanisms of probiotic action in vitro, in animal models and human clinical trials. The strains adhered to human epithelial cells, helped to maintain the barrier function and blocked the adhesion of a number of path ...201122146691
Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products.It is well-known that probiotics have a number of beneficial health effects in humans and animals, including the reduction of symptoms in lactose intolerance and enhancement of the bioavailability of nutrients. Probiotics have showed to possess antimutagenic, anticarcinogenic and hypocholesterolemic properties. Further, they were also observed to have antagonistic actions against intestinal and food-borne pathogens, to decrease the prevalence of allergies in susceptible individuals and to have i ...201122159887
Effects of intramammary inoculation of Lactobacillus perolens CRL1724 in lactating cows' udders.Bovine mastitis is the most important infectious disease on dairy farms. Conventional antibiotic therapy is often unsatisfactory and alternative treatments are continually under investigation. Lactobacillus (Lb.) perolens CRL 1724 and Lactobacillus plantarum CRL 1716 were previously isolated from milk of dairy cows and selected according to their potential probiotic properties. In the present work the in-vitro capacity of Lactobacillus strains to adhere to bovine teat canal epithelial cells (BTC ...201122077995
effect of dietary arginine on growth, intestinal enzyme activities and gene expression in muscle, hepatopancreas and intestine of juvenile jian carp (cyprinus carpio var. jian).the present study was conducted to test the hypothesis that dietary arginine promotes digestion and absorption capacity, and, thus, enhances fish growth. this improvement might be related to the target of rapamycin (tor) and eif4e-binding protein (4e-bp). a total of 1200 juvenile jian carp, cyprinus carpio var. jian, with an average initial weight of 6·33 (se 0·03) g, were fed with diets containing graded concentrations of arginine, namely, 9·8 (control), 12·7, 16·1, 18·5, 21·9 and 24·5 g argini ...201122013925
a reference proteomic database of lactobacillus plantarum cmcc-p0002.lactobacillus plantarum is a widespread probiotic bacteria found in many fermented food products. in this study, the whole-cell proteins and secretory proteins of l. plantarum were separated by two-dimensional electrophoresis method. a total of 434 proteins were identified by tandem mass spectrometry, including a plasmid-encoded hypothetical protein plp9000_05. the information of first 20 highest abundance proteins was listed for the further genetic manipulation of l. plantarum, such as construc ...201121998671
a combination of direct viable count and fluorescence in situ hybridization for specific enumeration of viable lactobacillus delbrueckii subsp. bulgaricus and streptococcus thermophilus.aims:  we have developed a direct viable count (dvc)-fish procedure for quickly and easily discriminating between viable and nonviable cells of lactobacillus delbrueckii subsp. bulgaricus and streptococcus thermophilus strains, the traditional yogurt bacteria. methods and results:  direct viable count method has been modified and adapted for lact. delbrueckii subsp. bulgaricus and strep. thermophilus analysis by testing different times of incubation and concentrations of dna-gyrase inhibitors. d ...201122188589
identification of lactobacillus spp. in colostrum from chilean mothers.the biodiversity of lactobacillus spp. in colostrum samples from 116 chilean mothers was analyzed by pcr and 16s rdna sequencing. lactobacilli were isolated in 55.3% of the samples, with concentrations of 3.33 +/- 0.55 (log cfu/ml). the predominant species were l. plantarum (64%), l. fermentum (16%) and l. pentosus (9%). 28% of the isolated strains were resistant to gastric ph and bile salts, suggesting that they could be used as probiotics.201122097291
site-specific protein labeling with amine-containing molecules using lactobacillus plantarum sortase.modification of proteins with small molecules is a widely used and powerful tool in biological research. enzymatic approaches are particularly promising because substrate specificity allows for site-specific modification. sortase a, a transpeptidase from staphylococcus aureus, cleaves between the t and g residues in the sequence lpxtg, and subsequently links the carboxyl group of the t residue to an amino group of n-terminal glycine oligomers by a native peptide bond. although gram-positive bact ...201121922670
cytotoxic and antimicrobial aporphine alkaloids from fissistigma poilanei (annonaceae) collected in vietnam.two new aporphine alkaloids: 8-hydroxy-9-methoxy-1,2-methylenedioxyaporphine (1) and 8-hydroxy-3,9-dimethoxy-1,2-methylenedioxyaporphine (2) were isolated from the ethyl acetate extract of fissistigma poilanei along with five known compounds: oxocrebanine (3), kuafumine (4), (2r,3r)-3',4',5,7-tetrahydroxydihydroflavonol-3-o-α-l-rhamnopyranoside (5), (+)-catechin 3-o-α-l-rhamnopyranoside (6) and quercetine 3,7-dimethoxy-3'-o-α-l-rhamnopyranosyl-(1 → 2)-β-d-glucopyranoside (7). these two new aporp ...201122077457
Age-related decline in macrophage and lymphocyte functions in mice and its alleviation by treatment with probiotic Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum.This study evaluated the effects of probiotic Dahi administration in ageing mice on macrophage and lymphocyte functions. Probiotic Dahi were prepared by co-culturing in buffalo milk (3% fat) Dahi bacteria (Lactococcus lactis ssp. cremoris NCDC-86 and Lc. lactis ssp. lactis biovar diacetylactis NCDC-60) along with Lactobacillus acidophilus LaVK2 (La-Dahi) or combined Lb. acidophilus and Bifidobacterium bifidum BbVK3 (LaBb-Dahi). Four groups of 12 mo old mice were fed for four months, with the sup ...201121871143
Effects of Different Sources of Fructans on Body Weight, Blood Metabolites and Fecal Bacteria in Normal and Obese non-diabetic and Diabetic Rats.Fructans contribute significantly to dietary fiber with beneficial effects on gastrointestinal physiology in healthy individuals and offer a promising approach to treating some diseases. Two experiments (Experiment 1 = rats with normal weight; Experiment 2 = obese rats) were developed to compare the effects of three fructan sources (Cichorium intybus L. Asteraceae, Helianthus tuberosus L. Asteraceae and Agave angustifolia ssp. tequilana Haw, Agavaceae) on body weight change, blood metabolites an ...201122210166
effect of fermented sea tangle on the alcohol dehydrogenase and acetaldehyde dehydrogenase in saccharomyces cerevisiae.sea tangle, a kind of brown seaweed, was fermented with lactobacillus brevis bj-20. the gamma-aminobutyric acid (gaba) content in fermented sea tangle (fst) was 5.56% (w/w) and gaba in total free amino acid of fst was 49.5%. the effect of fst on the enzyme activities and mrna protein expression of alcohol dehydrogenase (adh) and acetaldehyde dehydrogenase (aldh) involved in alcohol metabolism in saccharomyces cerevisiae was investigated. yeast was cultured in ypd medium supplemented with differe ...201121876367
Enteric commensal bacteria induce extracellular signal-regulated kinase pathway signaling via formyl peptide receptor-dependent redox modulation of dual specific phosphatase 3.The normal microbial occupants of the mammalian intestine are crucial for maintaining gut homeostasis, yet the mechanisms by which intestinal cells perceive and respond to the microbiota are largely unknown. Intestinal epithelial contact with commensal bacteria and/or their products has been shown to activate noninflammatory signaling pathways, such as extracellular signal-related kinase (ERK), thus influencing homeostatic processes. We previously demonstrated that commensal bacteria stimulate E ...201121921027
Genomic analysis reveals Lactobacillus sanfranciscensis as stable element in traditional sourdoughs.Sourdough has played a significant role in human nutrition and culture for thousands of years and is still of eminent importance for human diet and the bakery industry. Lactobacillus sanfranciscensis is the predominant key bacterium in traditionally fermented sourdoughs.The genome of L. sanfranciscensis TMW 1.1304 isolated from an industrial sourdough fermentation was sequenced with a combined Sanger/454-pyrosequencing approach followed by gap closing by walking on fosmids. The sequencing data r ...201121995419
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