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[direction of strategic use: a new classification of non-steroidal anti-inflammatory drugs based on reactivity with peroxidase].the pharmaceutical effects of non-steroidal anti-inflammatory drugs (nsaids) occur through the inhibition of prostaglandin h synthase (pghs). prostaglandin h2 is produced from arachidonic acid via peroxidase and cyclooxygenase cycles in pghs. nsaids exhibit different levels of reactivity in these reaction cycles. to prevent the development of side effect while maintaining the beneficial effects of drugs, a therapeutic strategy should be used. a new classification of nsaids has been proposed base ...201323474686
investigations of ferric heme cyanide photodissociation in myoglobin and horseradish peroxidase.the photodissociation of cyanide from ferric myoglobin (mbcn) and horseradish peroxidase (hrpcn) has definitively been observed. this has implications for the interpretation of ultrafast ir (helbing et al. biophys. j. 2004, 87, 1881-1891) and optical (gruia et al. biophys. j. 2008, 94, 2252-2268) studies that had previously suggested the fe-cn bond was photostable in mbcn. the photolysis of ferric mbcn takes place with a quantum yield of ~75%, and the resonance raman spectrum of the photoproduct ...201323472676
thermostable trypsin conjugates immobilized to biogenic magnetite show a high operational stability and remarkable reusability for protein digestion.in this work, magnetosomes produced by microorganisms were chosen as a suitable magnetic carrier for covalent immobilization of thermostable trypsin conjugates with an expected applicability for efficient and rapid digestion of proteins at elevated temperatures. first, a biogenic magnetite was isolated from magnetospirillum gryphiswaldense and its free surface was coated with the natural polysaccharide chitosan containing free amino and hydroxy groups. prior to covalent immobilization, bovine tr ...201323466477
supramolecular assembly of enzyme on functionalized graphene for electrochemical biosensing.the self-assembly of cyclodextrin (cd) functionalized graphene (gr) and adamantane-modified horseradish peroxidase (hrp-ada) by host-guest supramolecular interaction into novel nanostructures in aqueous solution is reported in the present study. electrochemical impedance spectroscopy and cyclic voltammetry were applied to characterize the self-assembly process and study the electrochemical behaviors of the immobilized proteins. uv-vis spectra indicated that the native structure of hrp was mainta ...201323455048
poly(dopamine) coated gold nanocluster functionalized electrochemical immunosensor for brominated flame retardants using multienzyme-labeling carbon hollow nanochains as signal amplifiers.an electrochemical, signal amplified immunosensor was developed to detect 3-bromobiphenyl (bbp) by using a bio-inspired polydopamine (pdop)/gold nanocluster (aunc) as the sensor platform and multienzyme-labeled carbon hollow nanochains as the signal amplifier. the self-polymerized dopamine membrane on the aunc-modified indium tin oxide (ito) electrode were characterized by scanning electron microscopy (sem), atomic force microscopy (afm), contact angle and electrochemical measurements. such pdop ...201323455046
in chemico evaluation of prohapten skin sensitizers: behavior of 2-methoxy-4-(¹³c)methylphenol in the peroxidase peptide reactivity assay (ppra) as an alternative to animal testing.in chemico methods, based on the assessment of a hapten's reactivity toward peptides, have been proposed as alternative methods for the assessment of the skin sensitizing potential of chemicals. however, even with these approaches showing promise, a major drawback is the activation of prohaptens, i.e. molecules needing a metabolic activation to become reactive and therefore sensitizing. recently, it has been proposed to couple an enzymatic activation step based on horseradish peroxidase (hrp)/hy ...201323454653
pentamines as substrate for human spermine oxidase.substrate activities of various linear polyamines to human spermine oxidase (hsmo) were investigated. the activities were evaluated by monitoring the amount of h2o2 released from sample polyamines by hsmo. h2o2 was measured by a hplc method that analyzed fluorescent dimers derived from the oxidation of homovanillic acid in the presence of horseradish peroxidase. six triamines were tested and were found not to be hsmo substrates. of sixteen tetramines tested, spermine (spm) was the most active su ...201323449327
precise sequence control in linear and cyclic copolymers of 2,5-bis(2-thienyl)pyrrole and aniline by dna-programmed assembly.a series of linear and cyclic, sequence controlled, dna-conjoined copolymers of aniline (ani) and 2,5-bis(2-thienyl)pyrrole (sns) were synthesized. in one approach, linear copolymers were prepared from complementary dna oligomers containing covalently attached sns and ani monomers. hybridization of the oligomers aligns the monomers in the major groove of the dna. treatment of the sns- and ani-containing duplexes with horseradish peroxidase (hrp) and h2o2 causes rapid and efficient polymerization ...201323448549
novel synthesis of super peroxidase mimetic polycrystalline mixed metal hexacyanoferrates nanoparticles dispersion.mixed metal-hexacyanoferrates (mhcf) have shown promising applications for technological development particularly in energy storage technologies, ferromagnetic materials, and electrode materials. however, the challenge of controlling their chemical synthesis, solubility, polycrystallinity and nanogeometry have restricted the practical implementation of mhcf in commercial designs. further, mhcfs have shown potential as peroxidase mimetics. there is still challenging demand to enhance the catalyti ...201323446316
detection of anticancer drug tamoxifen using biosensor based on polyaniline probe modified with horseradish peroxidase.amperometric biosensor based on horseradish peroxidase immobilized via glutaraldehyde on the polyaniline modified platinum electrode shows evidenced promising characteristics in detecting anticancer drug tamoxifen. the sensor was fabricated simply by adsorbing horseradish peroxidase enzyme on the electrode surface for which cyclic voltammetry was used to monitor the electro-catalytic reduction of tamoxifen under diffusion-adsorption controlled conditions. fourier transform infrared spectroscopy, ...201325427459
highly sensitive analysis of four hemeproteins by dynamically-coated capillary electrophoresis with chemiluminescence detector using an off-column coaxial flow interface.dynamic coating of the surface in capillary electrophoresis with chemiluminescence detection (ce-cl) using an off-column coaxial flow interface for the determination of four hemeproteins was developed. this method is based on the luminol-hydrogen peroxide reaction catalyzed by metalloproteins in alkaline medium. the experimental setup of the ce-cl system with the proposed off-column coaxial interface was evaluated by separation and detection of dopamine and catechol based on inhibition of the lu ...201323443524
superior performance of liposomes over enzymatic amplification in a high-throughput assay for myoglobin in human serum.myoglobin is one of several cardiac markers which become elevated in the blood following an acute myocardial infarction and can aid in the diagnosis of a heart attack. here, a sandwich immunoassay for myoglobin was developed, including a thorough optimization of fluorescent dye-encapsulating liposomes versus enzymatic amplification (alkaline phosphatase and horseradish peroxidase) at each step. the optimized microtiter plate-based assay was capable of detecting as low as 11.3 pg/ml myoglobin and ...201323443519
simultaneous determination of 13 fluoroquinolone and 22 sulfonamide residues in milk by a dual-colorimetric enzyme-linked immunosorbent assay.enzyme-linked immunosorbent assays (elisas) usually focus on the detection of a single analyte or a single group of analytes, e.g., fluoroquinolones or sulfonamides. however, it is often necessary to simultaneously monitor two classes of antimicrobial residues in different food matrixes. in this paper, we describe a dual-colorimetric elisa for the simultaneous detection of 13 fluoroquinolone and 22 sulfonamide residues. the limit of detection for fluoroquinolones and sulfonamides was 2.4 and 5.8 ...201323347187
synthetic cascades are enabled by combining biocatalysts with artificial metalloenzymes.enzymatic catalysis and homogeneous catalysis offer complementary means to address synthetic challenges, both in chemistry and in biology. despite its attractiveness, the implementation of concurrent cascade reactions that combine an organometallic catalyst with an enzyme has proven challenging because of the mutual inactivation of both catalysts. to address this, we show that incorporation of a d(6)-piano stool complex within a host protein affords an artificial transfer hydrogenase (athase) th ...201323344429
an ultrasensitive electrochemical aptasensor for thrombin based on the triplex-amplification of hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme and horseradish peroxidase decorated fete nanorods.in the present study, we fabricated an ultrasensitive sandwich-type electrochemical aptasensor for thrombin (tb) based on a triplex signal amplification strategy. the hemin/g-quadruplex horseradish peroxidase-mimicking dnazyme (hrp-dnazyme) as well as blocking reagent-horseradish peroxidase (hrp) and iron telluride nanorods (fete nrs) could simultaneously amplify the electrochemical signal of thionine (thi) in the presence of h(2)o(2). herein, fete nrs, as a newly discovered hrp-mimicking enzyme ...201323340527
detrimental effect of electromagnetic pulse exposure on permeability of in vitro blood-brain-barrier model.to study the effect of electromagnetic pulse (emp) exposure on permeability of in vitro blood-brain-barrier (bbb) model.201323336136
vagus nerve stimulation inhibits seizure activity and protects blood-brain barrier integrity in kindled rats with cortical dysplasia.this study investigates the effects of vagus nerve stimulation (vns) on seizure severity and blood-brain barrier (bbb) integrity in kindled rats with cortical dysplasia (cd).201323333826
electron transfer properties of dual self-assembled architectures based on specific recognition and electrostatic driving forces: its application to control substrate inhibition in horseradish peroxidase-based sensors.this work describes the synergistic combination of ionic self-assembly and recognition-directed assembly with the aim of creating highly functional bioelectrochemical interfaces compatible with the supramolecular design of a wide variety of biosensing platforms. a recently synthesized glycopolyelectrolyte constituted of polyallylamine bearing redox-active osmium complexes and glycosidic residues (lactose) is used to create a self-assembled structure with sodium dodecylsulfate. in turn, this supr ...201323331115
micropipet tip-based miniaturized electrochemical device combined with ultramicroelectrode and its application in immobilization-free enzyme biosensor.in this study, a simple miniaturized microliter electrochemical device was constructed using a disposable micropipet tip and a reproducible carbon fiber ultramicroelectrode. the novel electrochemical device set the electrochemical reaction in a micropipet tip containing an ultramicroelectrode. we investigated the feasibility of the designed electrochemical device by cyclic voltammetric measurements of redox probe. its application in an immobilization-free enzyme electrochemical biosensor was als ...201323331083
flow injection chemiluminescence immunoassay of microcystin-lr by using pei-modified magnetic beads as capturer and hrp-functionalized silica nanoparticles as signal amplifier.a rapid sandwiched immunoassay of microcystin-lr (mc-lr) in water is proposed with flow injection chemiluminescence detection. the magnetic beads (mbs) were first modified with polyethyleneimine (pei) by acylamide bond between the carboxyl group on the surface of mbs and the primary amine group in pei, followed by immobilizing of anti-mc-lr (ab1) onto pei with glutaraldehyde as linkage. the resulting ab1 modified mbs captured the target mc-lr in water, reacted with the horseradish peroxidase and ...201323330150
injectable hyaluronic acid-tyramine hydrogels incorporating interferon-α2a for liver cancer therapy.we report an injectable hydrogel system that incorporates interferon-α2a (ifn-α2a) for liver cancer therapy. ifn-α2a was incorporated in hydrogels composed of hyaluronic acid-tyramine (ha-tyr) conjugates through the oxidative coupling of tyr moieties with hydrogen peroxide (h2o2) and horseradish peroxidase (hrp). ifn-α2a-incorporated ha-tyr hydrogels of varying stiffness were formed by changing the h2o2 concentration. the incorporation of ifn-α2a did not affect the rheological properties of the ...201323328125
copper nanoclusters as peroxidase mimetics and their applications to h2o2 and glucose detection.copper nanoclusters (cu ncs) are found to possess intrinsic peroxidase-like activity for the first time. similar to nature peroxidase, they can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by h(2)o(2) to produce a nice blue color reaction. compared with horseradish peroxidase, cu ncs exhibits higher activity near neutral ph, which is beneficial for biological applications. the increase in absorbance caused by the cu ncs catalytic reaction allows the detection of h(2)o(2) in the range ...201323327949
simultaneous immunoassay analysis of plasma il-6 and tnf-α on a microchip.sandwich enzyme-linked immunosorbant assay (elisa) using a 96-well plate is frequently employed for clinical diagnosis, but is time-and sample-consuming. to overcome these drawbacks, we performed a sandwich elisa on a microchip. the microchip was made of cyclic olefin copolymer with 4 straight microchannels. for the construction of the sandwich elisa for interleukin-6 (il-6) or tumor necrosis factor-α (tnf-α), we used a piezoelectric inkjet printing system for the deposition and fixation of the ...201323326472
influence of different length spacers containing enzyme conjugate on functional parameters of progesterone elisa.in steroid enzyme immunoassay (eia), there is an increase or decrease of labeled steroid recognition by antibody due to homologous and heterologous combinations of enzyme conjugate with immunogen that affects sensitivity of the assay. we have introduced three to 18 atomic length linkers between enzyme and steroid moieties and studied their effects on functional parameters such as sensitivity, ed(50), and specificity of progesterone enzyme immunoassays. progesterone-3-carboxymethyloxime-bovine se ...201323323985
development of monoclonal antibodies and quantitative sandwich enzyme linked immunosorbent assay for the characteristic sialoglycoprotein of edible bird's nest.the article presents a sandwich enzyme linked immunosorbent assay (elisa) for identification of edible bird's nest. the characteristic sialoglycoproteins were found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and purified by liquid-phase isoelectric focusing (lief). according to the analysis, the molecular weight was 106-128 kda and the isoelectric point was ≤ph 3.0. two anti-characteristic sialoglycoprotein monoclonal antibodies were produced. the monoclonal antibodi ...201323323981
a label-free biosensor for electrochemical detection of femtomolar micrornas.a simple and ultrasensitive label-free microrna (mirna) biosensor, based on hybridized mirna-templated deposition of an insulating polymer film and electrochemical impedance spectroscopic detection, is described in this report. the biosensor is made of a monolayer of charge-neutral morpholino capture probes on an indium tin oxide (ito)-coated glass slide. upon hybridization, the neutral surface of the biosensor is converted to anionic by the hybridized mirna strands. the deposition of the insula ...201323323518
horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication.we report a method for preparing cell-laden hydrogel tubes. this method uses a coaxial double-orifice spinneret, simpler than triple-orifice spinnerets which have been used for preparing similar constructs. the intended application was to create a template for preparing filament-like structures composed of two heterogeneous living cell layers. an aqueous solution containing an alginate derivative possessing phenolic hydroxyl moieties (alg-ph), catalase and horseradish peroxidase (hrp) was extrud ...201323319520
in situ synthesized gold nanoparticles for direct electrochemistry of horseradish peroxidase.in order to construct a three-dimensional electrode, in situ electrochemical deposition of gold nanoparticles onto a gold electrode coated with a self-assembled monolayer of 3-mercaptopropionic acid (au np/mpa/au) was conducted. horseradish peroxidase (hrp) was then immobilized into this three-dimensional electrode, leading to the realization of direct electron transfer of hrp. scanning electron microscopy and electrochemical impedance spectroscopy were applied to characterize the electrode befo ...201323314493
immobilization of horseradish peroxidase by electrospun fibrous membranes for adsorption and degradation of pentachlorophenol in water.horseradish peroxidase (hrp) is successfully in situ encapsulated into the poly(d,l-lactide-co-glycolide) (plga)/peo-ppo-peo (f108) electrospun fibrous membranes (efms) by emulsion electrospinning. the adsorption and degradation of pentachlorophenol (pcp) by hrp-efms are investigated. the experimental results show that the sorption kinetic of pcp on efms follows the pseudo-second-order model, and the sorption capacity is as high as 44.69 mg g(-1). the sorption mechanisms of efms for pcp can be e ...201323295768
clinical and immunohistopathological aspects of venous ulcers treatment by low-intensity pulsed ultrasound (lipus).the immunological mechanisms that are triggered by low-intensity pulsed ultrasound (lipus) in wound healing are unknown. in the present study, experimental groups were used to assess the treatment of chronic venous ulcers with 30mw/cm(2) sata peripheral lipus three times per week compared to a daily treatment of 1% silver sulfadiazine (sdz). the ulcers of the sdz group (n=7) (g1) and lipus group (n=9) (g2) were photographed five times three months, and the images were analyzed using imagej softw ...201323294989
effect of artemin on structural transition of β-lactoglobulin.encysted embryos of artemia are exceptionally resistant to severe environmental stress. this resistance is thought to depend in part on the existence of a protein termed artemin. there is only little information about the function of artemin. it has been reported artemin is a thermostable protein with rna-binding ability. in addition, it reduces the extent of aggregation significantly and enhances the efficiency of refolding and activity recovery of carbonic anhydrase and horseradish peroxidase. ...201323291197
increased electrocatalyzed performance through dendrimer-encapsulated gold nanoparticles and carbon nanotube-assisted multiple bienzymatic labels: highly sensitive electrochemical immunosensor for protein detection.a highly sensitive electrochemical carcinoembryonic antigen (cea) immunosensor was fabricated by covalently immobilizing a monoclonal cea antibody (anti-cea, ab(1)) and a mediator (thionine, th) on a gold nanoparticle (aunp)-encapsulated dendrimer (den/aunp). multiwalled carbon nanotube (mwcnt)-supported secondary antibody (ab(2))-conjugated multiple bienzymes, glucose oxidase (gox), and horseradish peroxidase (hrp) (ab(2)/mwcnt/gox/hrp) were used as electrochemical labels. the highly sensitive ...201323289608
synthesis and antioxidant activity of o-alkyl selenocarbamates, selenoureas and selenohydantoins.the preparation of three different families of lipophilic organoselenium compounds (aryl- and sugar-derived selenoureas, o-alkyl selenocarbamates and selenohydantoins) has been carried out in order to evaluate their in vitro antioxidant profile, analyzing the influence of the selenium-containing functional group, and the substituents on the activity. title compounds have therefore been studied for the first time as free radical, hydrogen peroxide, alkyl peroxides and nitric oxide scavengers usin ...201323287366
metal ions control product specificity of isoprenyl diphosphate synthases in the insect terpenoid pathway.isoprenyl diphosphate synthases (idss) produce the ubiquitous branched-chain diphosphates of different lengths that are precursors of all major classes of terpenes. typically, individual short-chain idss (scidss) make the c10, c15, and c20 isoprenyl diphosphates separately. here, we report that the product length synthesized by a single scids shifts depending on the divalent metal cofactor present. this previously undescribed mechanism of carbon chain-length determination was discovered for a sc ...201323440195
manganese porphyrin-dsdna complex: a mimicking enzyme for highly efficient bioanalysis.manganese porphyrin (mntmpyp)-dsdna complex was reported as an excellent mimicking enzyme of peroxidase. it possessed high catalytic activity and much quicker catalytic kinetics and better stability with exposure to light irradiation and high temperature than both horseradish peroxidase and hemin/g-quadruplex dnazyme. the groove binding of mntmpyp to the dsdna scaffold efficiently maintained the catalytic activity of the mntmpyp center and improved its stability. by combining with an isothermal ...201323427829
a rapid and highly sensitive portable chemiluminescent immunosensor of carcinoembryonic antigen based on immunomagnetic separation in human serum.to detect a biomarker for lung cancer, carcinoembryonic antigen (cea), a highly sensitive, selective, rapid and portable immunosensor based on immunomagnetic separation and chemiluminescence immunoassay was introduced. a sandwich scheme assay has been utilized with horseradish peroxidase (hrp) labeled anti-cea antibody and immunomagnetic beads (imbs). the presence of target protein cea caused the formation of the sandwich structures (imbs-cea-hrp labeled antibody). imbs were applied to capture c ...201323427806
highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection.a noncompetitive immunoassay based on gold nanoparticles (aunps) amplified capillary electrophoresis (ce) chemiluminescence (cl) detection was developed for the determination of carcinoembryonic antigen (cea). in this method, citrate-modified aunps were conjugated with horseradish peroxidase (hrp) labeled cea antibody (ab*), and incubated with limited amount of cea antigen. cea-ab*-aunps complex and excess of ab*-aunps were then separated and quantified by ce with cl detection. highly sensitive ...201323422894
propofol and thiopental suppress amyloid fibril formation and gm1 ganglioside expression through the γ-aminobutyric acid a receptor.the incidence of alzheimer disease may increase after surgical interventions. amyloid β-protein (aβ) fibrillogenesis, which is closely related to alzheimer disease, is reportedly accelerated by exposure to anesthetics. however, the effects of gm1 ganglioside (gm1) on αβ fibrillogenesis have not yet been reported. the current study was designed to examine whether the anesthetics propofol and thiopental are associated with αβ assembly and gm1 expression on the neuronal cell surface.201323422796
switchable catalytic acrylamide hydrogels cross-linked by hemin/g-quadruplexes.copolymer chains consisting of acrylamide units and guanine (g)-containing oligonucleotide-tethered acrylamide units undergo, in the presence of k(+) ions, cross-linking by g-quadruplexes to yield a hydrogel. the hydrogel is dissociated upon addition of 18-crown-6 ether that traps the k(+) ions. reversible formation and dissociation of the hydrogel is demonstrated by the cyclic addition of k(+) ions and 18-crown-6 ether, respectively. formation of the hydrogel in the presence of hemin results in ...201323421921
integrated amperometric affinity biosensors using co2+-tetradentate nitrilotriacetic acid modified disposable carbon electrodes: application to the determination of β-lactam antibiotics.a novel strategy for the construction of disposable amperometric affinity biosensors is described in this work. the approach uses a recombinant bacterial penicillin binding protein (pbp) tagged by an n-terminal hexahistidine tail which was immobilized onto co(2+)-tetradentate nitrilotriacetic acid (nta)-modified screen-printed carbon electrodes (spces). the biosensor was employed for the specific detection and quantification of β-lactam antibiotics residues in milk, which was accomplished by mea ...201323421824
iron oxide magnetic nanoparticles with versatile surface functions based on dopamine anchors.the synthesis of multifunctional magnetic nanoparticles (mf-mps) is one of the most active research areas in advanced materials as their multifunctional surfaces allow conjugation of biological and chemical molecules, thus making it possible to achieve target-specific diagnostic in parallel to therapeutics. we report here a simple strategy to integrate in a one-step reaction several reactive sites onto the particles. the preparation of mf-mps is based on their simultaneous modification with diff ...201323420060
an amperometric affinity penicillin-binding protein magnetosensor for the detection of β-lactam antibiotics in milk.the preparation, characterization and performance evaluation of an amperometric affinity disposable magnetosensor, based on the use of a recombinant penicillin-binding protein (pbp) and screen-printed carbon electrodes (spces), for the specific detection and quantification of β-lactam antibiotic residues in milk are reported. the pbp was immobilized onto his-tag-isolation-modified magnetic beads (his-tag-isolation-mbs), and a direct competitive assay using a tracer with horseradish peroxidase (h ...201323420036
development of an amperometric biosensor based on peroxidases to quantify citrinin in rice samples.an amperometric biosensor based on horseradish peroxidase (ec1.11.1.7,h2o2-oxide-reductases) to determine the content of citrinin mycotoxin in rice samples is proposed by the first time. the method uses carbon paste electrodes filled up with multi-walled carbon nanotubes embedded in a mineral oil, horseradish peroxidase, and ferrocene as a redox mediator. the biosensor is covered externally with a dialysis membrane, which is fixed to the body side of the electrode with a teflon laboratory film, ...201323416359
study on the reaction mechanism and the static injection chemiluminescence method for detection of acetaminophen.acetaminophen, also called paracetamol, is found in tylenol, excedrin and other products as over-the-counter medicines. in this study, acetaminophen as a luminol signal enhancer was used in the chemiluminescence (cl) substrate solution of horseradish peroxidase (hrp) for the first time. the use of acetaminophen in the luminol-hrp-h2o2 system affected not only the intensity of the obtained signal, but also its kinetics. it was shown that acetaminophen was to be a potent enhancer of the luminol-hr ...201323408702
feeding barley grain-rich diets altered electrophysiological properties and permeability of the ruminal wall in a goat model.high-producing ruminants are commonly fed large amounts of concentrate to meet their high energy demands for rapid growth or high milk production. however, this feeding strategy can severely impair rumen functioning, leading to subacute ruminal acidosis. subacute ruminal acidosis might have consequences for electrophysiological properties by changing the net ion transfer and permeability of ruminal epithelia, which may increase the uptake of toxic compounds generated in the rumen into the system ...201323403198
role of nitric oxide in the chemistry and anticancer activity of etoposide (vp-16,213).originally identified as an innate cytotoxin, nitric oxide ((·)no) formation in tumors can influence chemotherapy and exacerbate cancer progression. here, we examined the hypothesis that (·)no generation contributes to cancer cell drug resistance toward the widely used anticancer drug etoposide (vp-16). the uv-vis spectrum of vp-16 was not changed by exposure of vp-16 to (·)no in aqueous buffer. in contrast, reddish-orange compound(s) characteristic of o-quinone- and nitroso-vp-16 were readily g ...201323402364
nanogold-enhanced graphene nanosheets as multienzyme assembly for sensitive detection of low-abundance proteins.a new electrochemical immunosensing protocol was designed for detection of carcinoembryonic antigen (cea, as a model protein) by using graphene-carried poly(o-phenylenediamine)/gold hybrid nanosheets (gnpgs) as signal tags on the hierarchical dendritic gold microstructures (hdgms)-modified glassy carbon electrode. to prepare the signal tags, poly(o-phenylenediamine) molecules were initially immobilized on the surface of graphene nanosheets via the π-stacking interaction. then gold nanoparticles ...201323399473
impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths.gelatin and alginate derivatives possessing phenolic hydroxyl moieties (gelatin-ph and alg-ph) were dissolved in aqueous solution and conjugated via horseradish peroxidase-catalyzed crosslinking, resulting in hydrogelation. the objective of creating the hydrogels was to prepare cell sheets and spherical tissues wrapped in living cell sheaths. an increase in the gelatin-ph content in the hydrogel improved cellular adhesion on the hydrogel surface but hindered degradability by alginate lyase. a hy ...201323395920
horseradish peroxidase-catalyzed synthesis of poly(thiophene-3-boronic acid) biocomposites for mono-/bi-enzyme immobilization and amperometric biosensing.we report here on a facile enzymatic polymerization protocol to prepare enzyme-poly(thiophene-3-boronic acid) (ptba) polymeric biocomposites (pbcs) for high-performance mono-/bi-enzyme amperometric biosensing. horseradish peroxidase (hrp)-catalyzed polymerization of thiophene-3-boronic acid (tba) monomer was conducted in aqueous solution containing hrp (or plus glucose oxidase (gox)) by either directly added or gox-glucose generated oxidant h2o2. the mono-/bi-enzyme amperometric biosensors were ...201323391705
monolithic capillary column based glycoproteomic reactor for high-sensitive analysis of n-glycoproteome.despite the importance of protein n-glycosylation in a series of biological processes, in-depth characterization of protein glycosylation is still a challenge due to the high complexity of biological samples and the lacking of highly sensitive detection technologies. we developed a monolithic capillary column based glycoproteomic reactor enabling high-sensitive mapping of n-glycosylation sites from minute amounts of sample. unlike the conventional proteomic reactors with only strong-cation excha ...201323384158
mixed immunoassay design for multiple chemical residues detection.in this research, a mixed immunoassay design for multiple chemical residues detection based on combined reverse competitive enzyme-linked immunosorbent assay (elisa) procedure was developed. this method integrated two reverse elisa reactions in one assay by labeling horseradish peroxidase to deoxynivalenol (don) and orbifloxacin. within this method, ic50 of the two mabs for each analyte we produced ranged from 23~68 ng ml(-1) for dons and 4.1~49 ng ml(-1) for quinolones (qns). the limit of detec ...201323380954
assessment of polyphenolic content, antioxidant activity, protection against ros-induced dna damage and anticancer activity of vitis vinifera stem extracts.grape extracts and wine have been studied widely due to their beneficial effects on human health. however, there are only few studies from grape stems extracts. therefore, the main objective of the present study was the assessment in stem extracts from greek vitis vinifera varieties of the total polyphenolic content (tpc), the identification of the polyphenols present in them, and the evaluation of their antioxidant activity, protection against ros-induced dna damage and inhibition of liver (hep ...201323380202
an ultrasensitive electrochemical immunosensor platform with double signal amplification for indole-3-acetic acid determinations in plant seeds.a label-free electrochemical immunosensor for ultra-sensitive detection of indole-3-acetic acid (iaa), a very important phytohormone, has been developed in this work. the detection strategy was mainly based on 4-aminophenylboronic acid, magnetic nanoparticles functionalized with horseradish peroxidase-conjugated goat anti-rat immunoglobulin g (hrp-igg-fe(3)o(4)) and rat monoclonal antibody against iaa-modified gold nanoparticles (anti-iaa-aunps). hrp-igg-aunps was covalently assembled on the ele ...201323377501
new nerve regeneration strategy combining laminin-coated chitosan conduits and stem cell therapy.nerve regeneration remains a difficult challenge due to the lack of safe and efficient matrix support. we designed a laminin (ln)-modified chitosan multi-walled nerve conduit combined with bone marrow stem cell (bmsc) grating to bridge a 10 mm long gap in the sciatic nerve of sprague-dawley rats. the repair outcome was monitored during 16 weeks after surgery. successful grafting of ln onto the chitosan film, confirmed by immunolocalization, significantly improved cell adhesion. in vivo study sho ...201323376237
graphene oxide in cetyltrimethylammonium bromide (ctab) reverse micelle: a befitting soft nanocomposite for improving efficiency of surface-active enzymes.herein, we report the successful inclusion of 2d allotrope of carbon, graphene oxide (go) in cetyltrimethylammonium bromide (ctab)/isooctane/n-hexanol/water reverse micelle without compromising the stability of water-in-oil (w/o) microemulsion. this newly developed self-assembled nanocomposites act as proficient host for surface-active enzymes, lipase, horseradish peroxidase (hrp), and soybean peroxidase (sbp). lipase activity within go-doped ctab reverse micelles remarkably improved by 3.8-fold ...201323374433
detection of subnanomolar melamine based on electrochemical accumulation coupled with enzyme colorimetric assay.based on the synergetic effect of the electrochemical accumulation process and the signal amplification of enzymes, a new sensitive method has been developed for the detection of subnanomolar melamine. there are two steps involved in the sensor construction process: (1) accumulation of melamine on an electrode by cyclic voltammetric method and (2) chemical coupling of horseradish peroxidase (hrp) with the accumulated melamine through the linkage of glutaraldehyde. the coupled hrp catalyzes the o ...201323373873
the application of chiral arginine and multi-walled carbon nanotubes as matrices to monitor hydrogen peroxide.the enantioselective interaction between horseradish peroxidase (hrp) and arginine enantiomers was investigated by electrochemical methods through studying the electrocatalytic activity of h2o2 biosensor, which was obtained through l-arginine or d-arginine functionalized multi-walled carbon nanotubes (d-arg-mwcnts or l-arg-mwcnts) immobilizing horseradish peroxidase (hrp) on glassy carbon electrode. cyclic voltammetric and chronoamperometry were used to characterize the properties of the biosens ...201323369834
a method for conducting highly sensitive microrna in situ hybridization and immunohistochemical analysis in pancreatic cancer.profiling experiments in whole tissue biopsies have linked altered expression of micrornas (mirnas) to different types of cancer, including pancreatic ductal adenocarcinoma (pdac). emerging evidence indicates that altered mirna expression can occur in different cellular compartments (cancer and non-cancer cells) in tumor lesions, and thus it is important to ascertain which specific cell type expresses a particulars mirna in pdac tissues. here, we describe a highly sensitive fluorescence-based is ...201323359149
enzyme-linked fab' fragment based competitive immunoassay for ovalbumin in hot-processed food.a hen's egg is one of the most common causes of food allergy. the allergen quantitation in hot-processed food is always a difficult task, because the protein in these samples will be denatured, insoluble, and degraded. this article presents a competitive enzyme linked immunosorbent assay (elisa) for the quantitation of ovalbumin in hot-processed food. its recovery was improved nearly two times by the assay method as compared with previous sandwich elisa. the heat and dl-dithiothreitol treated ov ...201323859790
tetra(p-tolyl)borate-functionalized solvent polymeric membrane: a facile and sensitive sensing platform for peroxidase and peroxidase mimetics.the determination of peroxidase activities is the basis for enzyme-labeled bioaffinity assays, peroxidase-mimicking dnazymes- and nanoparticles-based assays, and characterization of the catalytic functions of peroxidase mimetics. here, a facile, sensitive, and cost-effective solvent polymeric membrane-based peroxidase detection platform is described that utilizes reaction intermediates with different pka values from those of substrates and final products. several key but long-debated intermediat ...201323852960
chondrocalcin is internalized by chondrocytes and triggers cartilage destruction via an interleukin-1β-dependent pathway.chondrocalcin is among the most highly synthesized polypeptides in cartilage. this protein is released from its parent molecule, type ii pro-collagen, after secretion by chondrocytes. a participation of extracellular, isolated chondrocalcin in mineralization was proposed more than 25 years ago, but never demonstrated. here, exogenous chondrocalcin was found to trigger mmp13 secretion and cartilage destruction ex vivo in human cartilage explants and did so by modulating the expression of interleu ...201323851124
investigation of spin-trapping artifacts formed by the forrester-hepburn mechanism.free radical detection with esr spin trapping relies on the specific addition of the radical to nitrone/nitroso compounds. it also has been proposed that spin traps can react in biological systems to give false-positive results. for nitrone spin traps, the reaction with nucleophiles, first described by forrester and hepburn, has been discussed as the most critical source of artifacts. for artifact identification, the esr preincubation method may be used, which employs isotopically marked spin tr ...201323851031
trefoil factor 3 peptide regulates migration via a twist-dependent pathway in gastric cell.trefoil factor 3 (tff3) is a member of the tff-domain peptide family and essential in regulating cell migration and maintaining mucosal integrity in gastrointestinal tract. however, the role of tff3 and its downstream regulating mechanisms in cancer cell migration remain unclear. we previously reported that tff3 prolonged the up-regulation of twist protein to modulate il-8 secretion in intestinal epithelial cells. in this study, we investigated the role of twist protein in tff3-induced migration ...201323845905
chemiluminescence competitive indirect enzyme immunoassay for 20 fluoroquinolone residues in fish and shrimp based on a single-chain variable fragment.a chemiluminescent competitive indirect enzyme-linked immunosorbent assay, based on a mutant single-chain variable fragment (scfv), was developed to detect a broad range of fluoroquinolones (fqs) in fish and shrimp matrices. in this study, the best scfvc4a9h1_mut2 was adopted, which showed 10-fold improved affinity to sarafloxacin (sar), difloxacin (dif), and trovafloxacin (tro), while the affinity to other fqs was fully inherited from wild-type scfvc4a9h1. in the optimized generic test, scfvc4a ...201323842902
stress-derived corticotropin releasing factor breaches epithelial endotoxin tolerance.loss of the endotoxin tolerance of intestinal epithelium contributes to a number of intestinal diseases. the etiology is not clear. psychological stress is proposed to compromise the intestinal barrier function. the present study aims to elucidate the role of the stress-derived corticotropin releasing factor (crf) in breaching the established intestinal epithelial endotoxin tolerance.201323840363
integrated disposable electrochemical immunosensors for the simultaneous determination of sulfonamide and tetracycline antibiotics residues in milk.the design, preparation and analytical performance of a novel integrated amperometric immunosensor based on the immobilization of selective capture antibodies on the surface of protein g-modified screen-printed dual carbon electrodes (spdces) for the multiplexed determination of sulfonamide and tetracycline antibiotics residues in milk is reported in this work. protein g was covalently immobilized onto a 4-aminobenzoic acid (4-aba) film grafted on the disposable electrode, and a direct competiti ...201323835224
glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip.in this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. the formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. enz ...201323831561
magnetic nanoparticle-based immunosensor for electrochemical detection of hepatitis b surface antigen.an electrochemical immunosensor was developed for the detection of hepatitis b surface antigen (hbsag). the biotinylated hepatitis b surface antibody was immobilized on streptavidin magnetic nanoparticles and used for targeting the hbsag. by the addition of horseradish peroxidase conjugated with secondary antibody (hrp-hbsab), a sandwich-type immunoassay format was formed. aminophenol as substrate for conjugated hrp was enzymatically changed into 3-aminophenoxazone (3-apz). this electroactive en ...201323831477
an examination of cardiovascular collapse induced by eastern brown snake (pseudonaja textilis) venom.the pseudonaja genus (brown snakes) is widely distributed across australia and bites account for significant mortality. venom-induced consumption coagulopathy (vicc) and, less often, early cardiovascular collapse occur following envenoming by these snakes. we have previously examined possible mechanism(s) behind the early cardiovascular collapse following papuan taipan (oxyuranus scutellatus) envenoming. in the present study, we investigate early cardiovascular collapse in anaesthetized rats fol ...201323830990
proteomic analysis for testis of mice exposed to carbon ion radiation.this paper investigates the mechanism of action of heavy ion radiation (hir) on mouse testes. the testes of male mice subjected to whole body irradiation with carbon ion beam (0.5 and 4gy) were analyzed at 7days after irradiation. a two-dimensional gel electrophoresis approach was employed to investigate the alteration of protein expression in the testes. spot detection and matching were performed using the pdquest 8.0 software. a difference of more than threefold in protein quantity (normalized ...201323827780
high-throughput receptor-based assay for the detection of spirolides by chemiluminescence.the spirolides are marine toxins that belong to a new class of macrocyclic imines produced by dinoflagellates. in this study a previously described solid-phase receptor-based assay for the detection of spirolides was optimized for high-throughput screening and prevalidated. this method is based on the competition between 13-desmethyl spirolide c and biotin-α-bungarotoxin immobilized on a streptavidin-coated surface, for binding to nicotinic acetylcholine receptors. in this inhibition assay the a ...201323827412
neuroprotective effects of ngf, bdnf, nt-3 and gdnf on axotomized extraocular motoneurons in neonatal rats.neurotrophic factors delivered from target muscles are essential for motoneuronal survival, mainly during development and early postnatal maturation. it has been shown that the disconnection between motoneurons and their innervated muscle by means of axotomy produces a vast neuronal death in neonatal animals. in the present work, we have evaluated the effects of different neurotrophic factors on motoneuronal survival after neonatal axotomy, using as a model the motoneurons innervating the extrao ...201323827308
gold nanoparticle-composite nanofibers for enzymatic electrochemical sensing of hydrogen peroxide.robust composite nanofibers (nfs) are prerequisite for highly efficient electrochemical sensors. we report the electrochemical application of gold nanoparticle (au np)-composite nafion nfs using a facile electrospinning technique. owing to the uniform distribution and large surface area of the au nps in the nfs, the au np-composite electrodes gave rise to greatly improved electrochemical properties, compared to aunp-free composite electrodes. when they were employed as reservoirs for immobilizin ...201323820982
unique monoclonal antibodies specifically bind surface structures on human fetal erythroid blood cells.continuing efforts in development of non-invasive prenatal genetic tests have focused on the isolation of fetal nucleated red blood cells (nrbcs) from maternal blood for decades. because no fetal cell-specific antibody has been described so far, the present study focused on the development of monoclonal antibodies (mabs) to antigens that are expressed exclusively on fetal nrbcs.201323819989
new methods for alk status diagnosis in non-small-cell lung cancer: an improved alk immunohistochemical assay and a new, brightfield, dual alk ihc-in situ hybridization assay.the demonstration of anaplastic lymphoma kinase (alk) positivity in non-small-cell lung cancer (nsclc) has been hindered by the technical complexity and interpretative challenges of fluorescence in situ hybridization methods for detection of alk gene rearrangement and by the inadequate sensitivity of existing immunohistochemistry (ihc) methods for alk protein detection. in this study, we sought to increase the sensitivity of alk ihc detection and to develop a brightfield assay for concurrent det ...201323817194
single-cell lysis for visual analysis by electron microscopy.the stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges available techniques. transmission electron microscopy (tem) allows for the analysis of proteins at the single-molecule level. here, we present a system for single-cell lysis under light microscopy observation, followed by rapid uptake of the c ...201323816812
development and validation of a sensitive enzyme immunoassay (eia) for blood plasma cortisol in female cattle, buffaloes, and goats.a highly sensitive enzyme immunoassay (eia) that used the second antibody coating technique and the cortisol-horseradish peroxidase conjugate as a label for determination of free and total cortisol in blood plasma of dairy animals (cows, buffaloes, and goats) was developed. for biological validation of the eia, blood samples were collected from the animals at 48 and 24 h before and 0, 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, and 132 h after dexamethasone administration. the eia was performed di ...201323816261
power-free chip enzyme immunoassay for detection of prostate specific antigen (psa) in serum.a power-free, portable "chip eia" was designed to render the popular enzyme linked immunosorbent assay (elisa) more suitable for point-of-care testing. a number of microfluidic platforms have enabled miniaturization of the conventional microtitre plate elisa, however, they require external pumping systems, valves, and electric power supply. the chip eia platform has eliminated the need for pumps and valves through utilizing a simple permanent magnet and magnetic nanoparticles. the magnetic nanop ...201323811482
in vitro antioxidant and anti-inflammatory activities of extracts from potentilla recta and its main ellagitannin, agrimoniin.potentilla recta is one of the numerous cinquefoil species growing in poland. it is used in traditional medicine e.g. in the treatment of skin inflammation.201323811215
synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions.in this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral ph condition. the monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. the polycondensation of alkoxysilanes and copolymerization of organic monomer and vin ...201323807307
oral administration of chitin and chitosan prevents peanut-induced anaphylaxis in a murine food allergy model.peanut allergy is ige-mediated type-i hypersensitivity, and t helper 2 cytokines are central to those pathogenesis. we investigated the effects of the administration of chitin and chitosan on peanut-induced hypersensitivities in mouse food allergy models. chitin and chitosan protected mice against peanut-induced anaphylaxis reactions, and the peanut-specific ige production decreased by up to 47% with the administration of β-chitosan. the levels of il-5, il-13, and il-10 were significantly suppre ...201323806320
q-site inhibitor induced ros production of mitochondrial complex ii is attenuated by tca cycle dicarboxylates.the impact of complex ii (succinate:ubiquinone oxidoreductase) on the mitochondrial production of reactive oxygen species (ros) has been underestimated for a long time. however, recent studies with intact mitochondria revealed that complex ii can be a significant source of ros. using submitochondrial particles from bovine heart mitochondria as a system that allows the precise setting of substrate concentrations we could show that mammalian complex ii produces ros at subsaturating succinate conce ...201323800966
elucidation of carbohydrate molecular interaction mechanism of recombinant and native artinm.the quartz crystal microbalance (qcm) technique has been applied for monitoring the biorecognition of artinm lectins at low horseradish peroxidase glycoprotein (hrp) concentrations, using a simple kinetic model based on langmuir isotherm in previous work.18 the latter approach was consistent with the data at dilute conditions but it fails to explain the small differences existing in the jartinm and rartinm due to ligand binding concentration limit. here we extend this analysis to differentiate s ...201323799777
dioxin-induced acute cardiac mitochondrial oxidative damage and increased activity of atp-sensitive potassium channels in wistar rats.the environmental dioxin 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is classified as a group 1 human carcinogen and teratogenic agent. we hypothesize that tcdd-induced oxidative stress may also interfere with mitochondrial atp-sensitive potassium channels (mitokatp), which are known to regulate and to be regulated by mitochondrial redox state. we investigated the effects of an acute treatment of male wistar rats with tcdd (50 μg/kg i.p.) and measured the regulation of cardiac mitokatp. while the ...201323796802
injectable and biodegradable sugar beet pectin/gelatin hydrogels for biomedical applications.injectable hydrogels have advantages over pre-formed hydrogels in biomedical applications. in our previous study, we showed usefulness of sugar beet pectin (sbp) as an injectable gel material. however, the in vivo biodegradability of the gels was low because animals lack suitable hydrolytic enzymes of sbp. in this study we developed sbp-based injectable gels with higher in vivo biodegradability than the previous sbp gels by incorporating biodegradable gelatin into the latter. an aqueous solution ...201323796034
neuron-derived igg protects dopaminergic neurons from insult by 6-ohda and activates microglia through the fcγr i and tlr4 pathways.oxidative and immune attacks from the environment or microglia have been implicated in the loss of dopaminergic neurons of parkinson's disease. the role of igg which is an important immunologic molecule in the process of parkinson's disease has been unclear. evidence suggests that igg can be produced by neurons in addition to its traditionally recognized source b lymphocytes, but its function in neurons is poorly understood. in this study, extensive expression of neuron-derived igg was demonstra ...201323791745
specific roles for group v secretory pla₂ in retinal iron-induced oxidative stress. implications for age-related macular degeneration.iron accumulation and oxidative stress are hallmarks of retinas from patients with age-related macular degeneration (amd). we have previously demonstrated that iron-overloaded retinas are a good in vitro model for the study of retinal degeneration during iron-induced oxidative stress. in this model we have previously characterized the role of cytosolic phospholipase a2 (cpla2) and calcium-independent isoform (ipla2). the aim of the present study was to analyze the implications of group v secreto ...201323791636
electrochemical biosensors for on-chip detection of oxidative stress from cells.the production of reactive oxygen species (ros) in the body has been shown to play a significant role in the development and progression of numerous diseases. this makes it important to develop a method of detection for hydrogen peroxide (h2o2), the most stable ros. several methods such as the use of fluorescent probes and electrochemistry have been utilized in the past to detect the imbalance in ros levels generated from injured or stimulated cells. an imbalance in the levels of ros leads to a ...201323791096
photooxidation of amplex red to resorufin: implications of exposing the amplex red assay to light.the amplex red assay, a fluorescent assay for the detection of h2o2, relies on the reaction of h2o2, which, in the presence of horseradish peroxidase, oxidizes the colorless, nonfluorescent, amplex red with a 1:1 stoichiometry to form the colored, fluorescent resorufin. we have found that resorufin is artifactually formed when amplex red is exposed to light. this photochemistry is initiated by trace amounts of resorufin present in amplex red stock solutions. esr spin-trapping studies have demons ...201323791091
boronic acid-functionalized core-shell-shell magnetic composite microspheres for the selective enrichment of glycoprotein.in this work, core-shell-shell-structured boronic acid-functionalized magnetic composite microspheres fe3o4@sio2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (fe3o4@sio2@p(mma-co-vpba)) with a uniform size and fine morphology were synthesized. here, fe3o4 magnetic particles were prepared by a solvothermal reaction, whereas the fe3o4@sio2 microspheres with a core-shell structure were obtained by a sol-gel process. 3-(trimethoxysilyl) propyl methacrylate (mps)-modified fe3o4@sio2 was us ...201323924282
an amperometric biosensor utilizing a ferrocene-mediated horseradish peroxidase reaction for the determination of capsaicin (chili hotness).chili hotness is very much dependent on the concentration of capsaicin present in the chili fruit. a new biosensor based on a horseradish peroxidase enzyme-capsaicin reaction mediated by ferrocene has been successfully developed for the amperometric determination of chili hotness. the amperometric biosensor is fabricated based on a single-step immobilization of both ferrocene and horseradish peroxidase in a photocurable hydrogel membrane, poly(2-hydroxyethyl methacrylate). with mediation by ferr ...201323921830
caffeine and modafinil promote adult neuronal cell proliferation during 48 h of total sleep deprivation in rat dentate gyrus.it has been established that sleep deprivation (sd) reduces the proliferation of neuronal precursors in the adult hippocampus. it has also been reported that psychostimulant drugs modulate adult neurogenesis. we examined the modulatory role of two psychostimulant drugs modafinil and caffeine on adult neuronal cell proliferation (ncp) during 48 h of total sd. a novel automated cage shaking stimulus was used to induce sd based on animal activity. 5-bromo-2″-deoxyuridine (brdu; 50mg/kg/day i.p.) wa ...201323920241
dyrk1a overexpression decreases plasma lecithin:cholesterol acyltransferase activity and apolipoprotein a-i levels.down syndrome is caused by trisomy of all or part of human chromosome 21. individuals with down syndrome present some metabolic abnormalities involving lipoproteins, notably lower high-density lipoprotein levels associated with altered lecithin:cholesterol acyltransferase activity and apolipoprotein a-i levels. dyrk1a is a kinase overexpressed in down syndrome that can activate the stat3 pathway, which is involved in lecithin:cholesterol acyltransferase expression. therefore, we characterized th ...201323920041
the effects of hyperbaric air and hyperbaric oxygen on blood-brain barrier integrity in rats.hyperbaric oxygen (hbo) treatment yields conflicting results on blood-brain barrier (bbb) integrity under various pathological conditions and the effects of hbo on healthy brain is poorly understood. in this experimental study, the effects of hbo on bbb integrity were investigated in comparison with hyperbaric air (hba) in intact rats. four sessions of hba or hbo were applied to intact rats in 24h. bbb integrity was functionally and structurally evaluated by determining extravasation of evans bl ...201323920007
hydrogen sulfide attenuates neurodegeneration and neurovascular dysfunction induced by intracerebral-administered homocysteine in mice.high levels of homocysteine (hcy), known as hyperhomocysteinemia are associated with neurovascular diseases. h2s, a metabolite of hcy, has potent anti-oxidant and anti-inflammatory activities; however, the effect of h2s has not been explored in hcy (ic)-induced neurodegeneration and neurovascular dysfunction in mice. therefore, the present study was designed to explore the neuroprotective role of h2s on hcy-induced neurodegeneration and neurovascular dysfunction. to test this hypothesis we emplo ...201323912038
magnetic fe3o4@tio2 nanoparticles-based test strip immunosensing device for rapid detection of phosphorylated butyrylcholinesterase.an integrated magnetic nanoparticles-based test strip immunosensing device was developed for rapid and sensitive quantification of phosphorylated butyrylcholinesterase (bche), the biomarker of exposure to organophosphorous pesticides (op), in human plasma. in order to overcome the difficulty in scarce availability of op-specific antibody, here magnetic fe3o4@tio2 nanoparticles were used and adsorbed on the test strip through a small magnet inserted in the device to capture target op-bche through ...201323911770
glycans from avian influenza virus are recognized by chicken dendritic cells and are targets for the humoral immune response in chicken.to increase our understanding of the interaction between avian influenza virus and its chicken host, we identified receptors for putative avian influenza virus (aiv) glycan determinants on chicken dendritic cells. chicken dendritic cells (dcs) were found to recognize glycan determinants containing terminal αgalnac, galα1-3gal, glcnacβ1-4glcnacβ1-4glcnacβ (chitotriose) and galα1-2gal. infection of chicken dendritic cells with either low pathogenic (lp) or highly pathogenic (hp) aiv results in ele ...201323911401
modularity of biochemical filtering for inducing sigmoid response in both inputs in an enzymatic and gate.we report the first systematic study of designed two-input biochemical systems as information processing gates with favorable noise transmission properties accomplished by modifying the gate's response from a convex shape to sigmoid in both inputs. this is realized by an added chemical "filter" process, which recycles some of the output back into one of the inputs. we study a system involving the biocatalytic function of the enzyme horseradish peroxidase, functioning as an and gate. we consider ...201323906353
functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays.two types of viral nanoparticles were functionalized with target-specific antibodies and multiple copies of an enzymatic reporter (horseradish peroxidase). the particles were successfully integrated into an immunochromatographic assay detecting ms2 bacteriophage, a model for viral pathogens. the sensitivity of the assay was greatly superior to conventional gold nanoparticle lateral flow assays, and results could easily be evaluated, even without advanced lab instruments.201323905160
hrp-mediated polymerization forms tough nanocomposite hydrogels with high biocatalytic performance.this communication describes the mild and quick construction of tough nanocomposite hydrogels via a horseradish peroxidase-mediated radical polymerization for effectively immobilizing enzymes to attain high catalytic performance in various solvents.201323903817
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