Publications

[clinical and capillaroscopic evaluation of chronic uncomplicated venous insufficiency with procyanidins extracted from vitis vinifera].	the pharmacological treatment of non-complicated chronic venous insufficiency is a current and well-debated topic. the introduction of new products with action on the venous system, improved knowledge on the physiopathology of venous insufficiency and the possibility provided by new analytical instruments, have given new impulse to the consolidation of the clinical value of phlebotonics in this indication.	2013	10356940
chromium and nickel in roadside grapes.	chromium and nickel concentration in roadside unwashed wine grapes and leaves is considered as a function of the distance from the suspected source, an isolated straight road. the analyses were performed with electrothermal atomization in a graphite furnace. the decrease in the concentration of the element can be described by means of a three-parameter exponential function c = a + (b - a)exp(-cd), which allows the determination of the asymptotic level far from the emitting source (c infinity) an ...	2013	2924978
[leukocyanidines and collagenases: in vitro enzyme inhibition activity].	the matrix metalloproteinases (mmps) play a key role in the extracellular matrix turnover. this protein family has been involved in some ocular pathologies such as glaucoma, diabetic retinopathy, macular degeneration, vitreous degeneration and corneal stroma ulceration cleaving all the matrix components. in the present study we evaluated the action of leucocyanidin from vitis vinifera seeds as non toxic inhibitor of these proteinases.	2014	10605164
assessment of cymbopogon citratus (dc.) stapf essential oil as herbal preservatives based on antifungal, antiaflatoxin, and antiochratoxin activities and in vivo efficacy during storage.	thirty-five randomly collected samples of stored table grapes (vitis vinifera l.) from different markets of gorakhpur city, uttar pradesh, india, revealed occurrence of 11 types of fungi. of which, aspergillus flavus, aspergillus niger, and aspergillus ochraceus were dominant causing severe decay of grapes with 58%, 52%, and 67% incidence, respectively. on screening of 15 essential oils at 0.33 μl/ml, cymbopogon citratus oil caused 100% mycelial inhibition against aforesaid dominant fungi. oil w ...	2014	24547889
the xylella fastidiosa pd1063 protein is secreted in association with outer membrane vesicles.	xylella fastidiosa is a gram-negative, xylem-limited plant pathogenic bacterium that causes disease in a variety of economically important agricultural crops including pierce's disease of grapevines. xylella fastidiosa biofilms formed in the xylem vessels of plants play a key role in early colonization and pathogenicity by providing a protected niche and enhanced cell survival. here we investigate the role of xylella fastidiosa pd1063, the predicted ortholog of xanthomonas oryzae pv. oryzae pxo_ ...	2014	25426629
bacteria isolated from roots and rhizosphere of vitis vinifera retard water losses, induce abscisic acid accumulation and synthesis of defense-related terpenes in in vitro cultured grapevine.	eleven bacterial strains were isolated at different soil depths from roots and rhizosphere of grapevines from a commercial vineyard. by 16s rrna gene sequencing 10 different genera and 8 possible at species level were identified. from them, bacillus licheniformis rt4m10 and pseudomonas fluorescens rt6m10 were selected according to their characteristics as plant growth promoting rhizobacteria (pgpr). both produced abscisic acid (aba), indole-3-acetic acid (iaa) and the gibberellins a1 and a3 in c ...	2014	24118032
effect of thymol and linalool fumigation on postharvest diseases of table grapes.	several postharvest diseases of table grapes (vitis vinifera) occur during storage, and gray mold rot is a particularly severe disease because the causal agent, botrytis cinerea, grows at temperatures as low as 0℃. other postharvest diseases, such as those caused by penicillium spp. and aspergillus spp., also often lead to deterioration in the quality of table grapes after harvest. the use of plant essential oils such as thymol and linalool, to reduce postharvest diseases in several kinds of fru ...	2014	25346603
the sweet family of sugar transporters in grapevine: vvsweet4 is involved in the interaction with botrytis cinerea.	during plant development, sugar export is determinant in multiple processes such as nectar production, pollen development and long-distance sucrose transport. the plant sweet family of sugar transporters is a recently identified protein family of sugar uniporters. in rice, sweet transporters are the target of extracellular bacteria, which have evolved sophisticated mechanisms to modify their expression and acquire sugars to sustain their growth. here we report the characterization of the sweet f ...	2014	25246444
the grapevine flagellin receptor vvfls2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium burkholderia phytofirmans and plant pathogenic bacteria.	• the role of flagellin perception in the context of plant beneficial bacteria still remains unclear. here, we characterized the flagellin sensing system flg22-flagellin sensing 2 (fls2) in grapevine, and analyzed the flagellin perception in the interaction with the endophytic plant growth-promoting rhizobacterium (pgpr) burkholderia phytofirmans. • the functionality of the grapevine fls2 receptor, vvfls2, was demonstrated by complementation assays in the arabidopsis thaliana fls2 mutant, which ...	2014	24491115
mode of action of a fatty acid-based natural product to control botrytis cinerea in grapes.	to investigate the efficacy and mode of action of the fatty acid-based product foodcoat(®) (fc) against botrytis cinerea.	2014	24387715
purification of antibiotics from the biocontrol agent streptomyces anulatus s37 by centrifugal partition chromatography.	a novel actinomycete strain, streptomyces anulatus s37, has been isolated from the rhizosphere of healthy moroccan vitis vinifera on the basis on its ability to promote grapevine growth and to induce natural defences against various phytopathogens. in the present work, the main bioactive metabolites produced by s. anulatus s37 were isolated. a crude n-buoh extract of the s37 fermentation broth was firstly partitioned in a biphasic solvent system composed of n-heptane, methanol, and water (5:1.5: ...	2014	24291716
osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to botrytis cinerea.	abiotic factors inducing osmotic stress can influence the plant immune response and resistance to pathogen infections. in this study, the effect of polyethylene glycol (peg)- and sucrose-induced osmotic stress on polyamine (pa) homeostasis and the basal immune response in grapevine plantlets before and after botrytis cinerea infection was determined. pharmacological approaches were also addressed to assess the contribution of osmotic stress-induced pa oxidation to the regulation of defence respo ...	2014	24170740
suppression of botrytis cinerea on necrotic grapevine tissues by early-season applications of natural products and biological control agents.	necrotic tissues within grape (vitis vinifera) bunches represent an important source of botrytis cinerea inoculum for botrytis bunch rot (bbr) at harvest in vineyards. this research quantified the incidence of b. cinerea on necrotic floral and fruit tissues and the efficacy of biologically based treatments for suppression of b. cinerea secondary inoculum within developing bunches.	2014	23744713
in vitro and in vivo evaluation of the antioxidant and prooxidant activity of phenolic compounds obtained from grape (vitis vinifera) pomace.	the antioxidant and/or prooxidant ability of extracts obtained from wine waste were analyzed using in vitro and in vivo assays. cyclic voltammetry was used as the in vitro assay to determine the antioxidant and/or prooxidant properties and, the in vivo effect on mycelial growth of the fungus botrytis cinerea was evaluated. in addition, the prooxidant activity was evaluated by intracellular oxidation of compound 2,7-dichlorodihydrofluorescein diacetate (dcfh-da) in b. cinerea. the extracts used i ...	2014	25521116
the crucial role of φ- and k-segments in the in vitro functionality of vitis vinifera dehydrin dhn1a.	dehydrins (dhns), group ii lea (late embryogenesis abundant) proteins, are among the most commonly observed proteins which accumulate in plants in response to cold and any other environmental factors, causing the dehydration of cells. in previous studies, we isolated a ysk2-type vvcdhn1a gene from table grapes (vitis vinifera cv. cardinal) which presented two spliced variants (the spliced, dhn1a_s and the unspliced, dhn1a_u). their expression was induced by low temperature storage and co2, altho ...	2014	25457499
pyrosequencing detects human and animal pathogenic taxa in the grapevine endosphere.	generally, plants are not considered as hosts for human and animal pathogens (hap). the recent produce-associated outbreaks of food-borne diseases have drawn attention toward significant deficiencies in our understanding of the ecology of hap, and their potential for interkingdom transfer. to examine the association of microorganisms classified as hap with plants, we surveyed the presence and distribution of hap bacterial taxa (henceforth hapt, for brevity's sake) in the endosphere of grapevine ...	2014	25071740
genome and transcriptome analysis of the grapevine (vitis vinifera l.) wrky gene family.	the plant wrky gene family represents an ancient and complex class of zinc-finger transcription factors (tfs) that are involved in the regulation of various physiological processes, such as development and senescence, and in plant response to many biotic and abiotic stresses. despite the growing number of studies on the genomic organisation of wrky gene family in different species, little information is available about this family in grapevine (vitis vinifera l.). in the present study, a total n ...	2014	26504535
comprehensive characterization and rna-seq profiling of the hd-zip transcription factor family in soybean (glycine max) during dehydration and salt stress.	the homeodomain leucine zipper (hd-zip) transcription factor family is one of the largest plant specific superfamilies, and includes genes with roles in modulation of plant growth and response to environmental stresses. many hd-zip genes are characterized in arabidopsis (arabidopsis thaliana), and members of the family are being investigated for abiotic stress responses in rice (oryza sativa), maize (zea mays), poplar (populus trichocarpa) and cucumber (cucmis sativus). findings in these species ...	2014	25362847
evolution and expression analysis of the grape (vitis vinifera l.) wrky gene family.	wrky proteins comprise a large family of transcription factors that play important roles in plant defence regulatory networks, including responses to various biotic and abiotic stresses. to date, no large-scale study of wrky genes has been undertaken in grape (vitis vinifera l.). in this study, a total of 59 putative grape wrky genes (vvwrky) were identified and renamed on the basis of their respective chromosome distribution. a multiple sequence alignment analysis using all predicted grape wrky ...	2014	24510937
unravelling the diversity of grapevine microbiome.	vitis vinifera is one of the most widely cultivated fruit crops with a great economic impact on the global industry. as a plant, it is naturally colonised by a wide variety of both prokaryotic and eukaryotic microorganisms that interact with grapevine, having either beneficial or phytopathogenic effects, who play a major role in fruit yield, grape quality and, ultimately, in the evolution of grape fermentation and wine production. therefore, the objective of this study was to extensively charact ...	2014	24454903
rna-seq profile of flavescence dorée phytoplasma in grapevine.	the phytoplasma-borne disease flavescence dorée is still a threat to european viticulture, despite mandatory control measures and prophylaxis against the leafhopper vector. given the economic importance of grapevine, it is essential to find alternative strategies to contain the spread, in order to possibly reduce the current use of harmful insecticides. further studies of the pathogen, the vector and the mechanisms of phytoplasma-host interactions could improve our understanding of the disease. ...	2014	25495145
metabolic and transcript analysis of the flavonoid pathway in diseased and recovered nebbiolo and barbera grapevines (vitis vinifera l.) following infection by flavescence dorée phytoplasma.	flavescence dorée phytoplasma (fdp) infections seriously affect production and survival of grapevine. we analysed the changes in the flavonoid pathway occurring in two red cultivars, the highly susceptible barbera and the less susceptible nebbiolo, following fdp infection. a combination of metabolic and transcript analyses was used to quantify flavonoid compounds and expression of a set of genes involved in their biosynthesis. quantification of anthocyanins, flavonols, proanthocyanidins and rela ...	2014	24689527
generation of expressed sequence tags under cadmium stress for gene discovery and development of molecular markers in chickpea.	chickpea is the world's third most important legume crop and belongs to fabaceae family but suffered from severe yield loss due to various biotic and abiotic stresses. development of modern genomic tools such as molecular markers and identification of resistant genes associated with these stresses facilitate improvement in chickpea breeding towards abiotic stress tolerance. in this study, 1597 high-quality expressed sequence tags (ests) were generated from a cdna library of variety pusa 1105 roo ...	2014	24414095
rapid shoot-to-root signalling regulates root hydraulic conductance via aquaporins.	we investigated how root hydraulic conductance (normalized to root dry weight, lo ) is regulated by the shoot. shoot topping (about 30% reduction in leaf area) reduced lo of grapevine (vitis vinifera l.), soybean (glycine max l.) and maize (zea mays l.) by 50 to 60%. more detailed investigations with soybean and grapevine showed that the reduction in lo was not correlated with the reduction in leaf area, and shading or cutting single leaves had a similar effect. percentage reduction in lo was la ...	2014	23926961
weissella uvarum sp. nov., isolated from wine grapes.	two bacterial strains (b18bm42(t) and b18nm6) were recovered during a study of bacterial diversity on wine grapes (vitis vinifera l.) from the nemea region in greece. phylogenetic analysis based on 16s rrna gene sequences placed the two strains within the genus weissella, and found them to be most closely related to weissella minor nric 1625(t) followed by weissella viridescens nric 1536(t) (99.1 and 98.9% sequence similarity, respectively). the level of dna-dna relatedness between strains b18nm ...	2014	25180092
differential production of phytotoxins from phomopsis sp. from grapevine plants showing esca symptoms.	nine strains of the fungus phomopsis spp. were isolated from a vineyard showing decline from the disease esca. strains were screened for their ability to produce secondary metabolites showing chemical diversity. the culture extracts of each strain were analyzed by liquid chromatography-ultraviolet-diode array detection-mass spectrometry. three strains were selected for the isolation and characterization of eight of the major metabolites. structures were elucidated by spectroscopic analyses inclu ...	2014	25070068
tydeid and triophtydeid mites (acari: tydeoidea) associated with grapevine (vitaceae: vitis spp.) in brazil, with the descriptions of species of prelorryia (andré, 1980) and tydeus koch, 1835.	we report on the tydeidae and triophtydeidae associated with grapevine (vitis labrusca l. and vitis vinifera l.) in rio grande do sul state, brazil, and describe a new species of prelorryia, prelorryia labrusca sp. nov., and two new species of tydeus, tydeus manoi sp. nov. and tydeus riopardensis sp. nov. brachytydeus formosa (cooreman), b. podocarpa (baker), neolorryia pandana (baker), tydeus californicus (banks), pretydeus henriandrei kaźmierski and triophtydeus lebruni (andré) are recorded an ...	2014	24943444
phytophthora niederhauserii sp. nov., a polyphagous species associated with ornamentals, fruit trees and native plants in 13 countries.	a non-papillate, heterothallic phytophthora species first isolated in 2001 and subsequently from symptomatic roots, crowns and stems of 33 plant species in 25 unrelated botanical families from 13 countries is formally described here as a new species. symptoms on various hosts included crown and stem rot, chlorosis, wilting, leaf blight, cankers and gumming. this species was isolated from australia, hungary, israel, italy, japan, the netherlands, norway, south africa, spain, taiwan, turkey, the u ...	2014	24871599
morphological and phylogenetic identification of botrytis sinoviticola, a novel cryptic species causing gray mold disease of table grapes (vitis vinifera) in china.	seventy-five isolates of botrytis collected from table grapes (vitis vinifera) with gray mold symptoms in china were identified based on morpho-cultural characteristics on potato dextrose agar (20 c) and/or phylogenetic analysis using the sequences of three nuclear genes (g3pdh, hsp60, rpb2). isolates of different species of botrytis were compared with fenhexamid sensitivity, bc-hch gene-rflp haplotyping and pathogenicity to v. vinifera. the 75 isolates comprise two species, b. cinerea (63 isola ...	2014	24396103
profiling the main cell wall polysaccharides of grapevine leaves using high-throughput and fractionation methods.	vitis species include vitis vinifera, the domesticated grapevine, used for wine and grape agricultural production and considered the world's most important fruit crop. a cell wall preparation, isolated from fully expanded photosynthetically active leaves, was fractionated via chemical and enzymatic reagents; and the various extracts obtained were assayed using high-throughput cell wall profiling tools according to a previously optimized and validated workflow. the bulk of the homogalacturonan-ri ...	2014	24274496
fingerprinting of anthocyanins from grapes produced in brazil using hplc-dad-ms and exploratory analysis by principal component analysis.	the anthocyanin profile was studied for 11 types of grapes of different varieties and geographical origins, including 10 wine grape varieties (vitis vinifera) and one hybrid variety. twenty anthocyanins were identified by means of the absorbance spectrum and fragmentation pattern by tandem mass spectrometry. the multivariate method of principal component analysis (pca) was used to evaluate differences amongst the cultivars. the results show the hybrid grape separated from a cluster represented b ...	2014	24128494
elucidation of the inhibitory effect of phytochemicals with kir6.2 wild-type and mutant models associated in type-1 diabetes through molecular docking approach.	among all serious diseases globally, diabetes (type 1 and type 2) still poses a major challenge to the world population. several target proteins have been identified, and the etiology causing diabetes has been reasonably well studied. but, there is still a gap in deciding on the choice of a drug, especially when the target is mutated. mutations in the kcnj11 gene, encoding the kir6.2 channel, are reported to be associated with congenital hyperinsulinism, having a major impact in causing type 1 d ...	2014	25705171
the role of phytochemicals as micronutrients in health and disease.	intake of dietary phytochemicals has frequently been associated with health benefits. noninfectious diseases including cardiovascular disease (cvd), cancer and diabetes are major causes of death, whereas dementia cases are also increasing to 'epidemic' proportion. this review will focus on recent progress on mechanisms underlying the potential role of dietary phytochemicals in cvd, diabetes, cancer and dementia, with consideration of the latest clinical data.	2014	25252018
dentin biomodification potential depends on polyphenol source.	although proanthocyanidins (pacs) modify dentin, the effectiveness of different pac sources and the correlation with their specific chemical composition are still unknown. this study describes the chemical profiling of natural pac-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (uhplc) to determine the overall composition of these extracts and, in parallel, comprehensively evaluate their effect on dentin properties. the total polyphenol content of the extr ...	2014	24574140
antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa.	grape extracts of the greek species vitis vinifera possess potent antioxidant properties in vitro. the freeze/thaw process and the preparation of semen during assisted reproductive techniques can adversely affect the functional integrity of spermatozoa. the objective was to assess the effect of three different concentrations (1 μg ml(-1), 2 μg ml(-1) and 5 μg ml(-1)) of a polyphenol-rich grape pomace extract on motility, viability, acrosomal and lipid peroxidation status of thawed bovine spermat ...	2014	25984501
study of leaf metabolome modifications induced by uv-c radiations in representative vitis, cissus and cannabis species by lc-ms based metabolomics and antioxidant assays.	uv-c radiation is known to induce metabolic modifications in plants, particularly to secondary metabolite biosynthesis. to assess these modifications from a global and untargeted perspective, the effects of the uv-c radiation of the leaves of three different model plant species, cissus antarctica vent. (vitaceae), vitis vinifera l. (vitaceae) and cannabis sativa l. (cannabaceae), were evaluated by an lc-hrms-based metabolomic approach. the approach enabled the detection of significant metabolite ...	2014	25197936
an optimized procedure for plant recovery from somatic embryos significantly facilitates the genetic improvement of vitis.	plant regeneration from grapevine (vitis spp.) via somatic embryogenesis typically is poor. recovery of plants from vitis rotundifolia michx. (muscadine grape) is particularly problematic due to extremely low efficiency, including extended culture durations required for embryo-plant conversion. poor plant recovery is an obstacle to the selection of improved genetically modified lines. somatic embryos (ses) of v. rotundifolia cultivar delicious (del-hs) and vitis vinifera l cultivar thompson seed ...	2014	26504540
integrated network analysis identifies fight-club nodes as a class of hubs encompassing key putative switch genes that induce major transcriptome reprogramming during grapevine development.	we developed an approach that integrates different network-based methods to analyze the correlation network arising from large-scale gene expression data. by studying grapevine (vitis vinifera) and tomato (solanum lycopersicum) gene expression atlases and a grapevine berry transcriptomic data set during the transition from immature to mature growth, we identified a category named "fight-club hubs" characterized by a marked negative correlation with the expression profiles of neighboring genes in ...	2014	25490918
the basic leucine zipper transcription factor abscisic acid response element-binding factor2 is an important transcriptional regulator of abscisic acid-dependent grape berry ripening processes.	in grape (vitis vinifera), abscisic acid (aba) accumulates during fruit ripening and is thought to play a pivotal role in this process, but the molecular basis of this control is poorly understood. this work characterizes abscisic acid response element-binding factor2 (vvabf2), a grape basic leucine zipper transcription factor belonging to a phylogenetic subgroup previously shown to be involved in aba and abiotic stress signaling in other plant species. vvabf2 transcripts mainly accumulated in t ...	2014	24276949
identification, classification and differential expression of oleosin genes in tung tree (vernicia fordii).	triacylglycerols (tag) are the major molecules of energy storage in eukaryotes. tag are packed in subcellular structures called oil bodies or lipid droplets. oleosins (ole) are the major proteins in plant oil bodies. multiple isoforms of ole are present in plants such as tung tree (vernicia fordii), whose seeds are rich in novel tag with a wide range of industrial applications. the objectives of this study were to identify ole genes, classify ole proteins and analyze ole gene expression in tung ...	2014	24516650
evaluation of five protocols for dna extraction from leaves of malus sieversii, vitis vinifera, and armeniaca vulgaris.	leaves of malus sieversii, vitis vinifera, and armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality dna extraction performance. in this study, five extraction protocols were compared for their ability to produce good quality dna from fresh and dried (with silica gel) leaves of these species. the modified protocol of dellaporta et al., using polyvinylpyrrolidone to bind the phenolic compounds and a high molar concentration of potassium acetate to i ...	2014	24634185
identification of new flavonols in hybrid grapes by combined liquid chromatography-mass spectrometry approaches.	grape flavonols are involved in the phenomenon of copigmentation in red wines. these compounds are characterised by nutraceutical properties, have antioxidant activity and are studied for chemotaxonomy of grapes. in general, hybrid grapes are characterised by presence of polyphenols often qualitatively and quantitatively different from vitis vinifera varieties. in this work, flavonols of 34 hybrid grape varieties (22 red and 12 white) produced by crossing of v. vinifera, vitis riparia, vitis lab ...	2014	24912722
low-dose irradiation can be used as a phytosanitary treatment for fresh table grapes.	grapes (vitis vinifera var. sugraone and vitis labrusca var. crimson seedless) were treated with 400, 600, and 800 gy and the effects on physicochemical factors were measured alongside sensory testing during 3 wk of storage. significant changes in texture and color with irradiation and age were measured but little visual difference was seen between control and irradiated grapes. however, age had a greater effect on firmness than irradiation for sugraone grapes. irradiation did not significantly ...	2014	24460773
micrornas in fruit trees: discovery, diversity and future research directions.	since the first description of micrornas (mirnas) 20 years ago, the number of mirnas identified in different eukaryotic organisms has exploded, largely due to the recent advances in dna sequencing technologies. functional studies, mostly from model species, have revealed that mirnas are major post-transcriptional regulators of gene expression in eukaryotes. in plants, they are implicated in fundamental biological processes, from plant development and morphogenesis, to regulation of plant pathoge ...	2014	24750383
a comprehensive review of plants and their active constituents with wound healing activity in traditional iranian medicine.	wound healing is a complex cascade of events with various cellular and biochemical processes that result in reconstruction and regeneration of damaged tissue. the objective of the current study was to scientifically evaluate the medicinal plants said to produce wound healing activity in traditional iranian medicine (tim).	2014	25856320
flowering as the most highly sensitive period of grapevine (vitis vinifera l. cv mourvèdre) to the botryosphaeria dieback agents neofusicoccum parvum and diplodia seriata infection.	botryosphaeria dieback is a fungal grapevine trunk disease that currently represents a threat for viticulture worldwide because of the important economical losses due to reduced yield of affected plants and their premature death. neofusicoccum parvum and diplodia seriata are among the causal agents. vine green stems were artificially infected with n. parvum or d. seriata at the onset of three different phenological stages (g stage (separated clusters), flowering and veraison). highest mean lesio ...	2014	24886812
extracellular compounds produced by fungi associated with botryosphaeria dieback induce differential defence gene expression patterns and necrosis in vitis vinifera cv. chardonnay cells.	three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. the different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. one hypothesis is tha ...	2014	24752796
reference gene validation for quantitative rt-pcr during biotic and abiotic stresses in vitis vinifera.	grapevine is one of the most cultivated fruit crop worldwide with vitis vinifera being the species with the highest economical importance. being highly susceptible to fungal pathogens and increasingly affected by environmental factors, it has become an important agricultural research area, where gene expression analysis plays a fundamental role. quantitative reverse transcription polymerase chain reaction (qrt-pcr) is currently amongst the most powerful techniques to perform gene expression stud ...	2014	25340748
functions of eds1-like and pad4 genes in grapevine defenses against powdery mildew.	the molecular interactions between grapevine and the obligate biotrophic fungus erysiphe necator are not understood in depth. one reason for this is the recalcitrance of grapevine to genetic modifications. using defense-related arabidopsis mutants that are susceptible to pathogens, we were able to analyze key components in grapevine defense responses. we have examined the functions of defense genes associated with the salicylic acid (sa) pathway, including enhanced disease susceptibility 1 (eds1 ...	2014	25107649
evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic dna markers associated with the resistant trait in chinese wild vitis.	the resistance of wild vitis germplasm, including chinese and american wild vitis and vitis vinifera cultivars, to powdery mildew (uncinula necator burr.) was evaluated for two consecutive years under natural conditions. most of the chinese and north american species displayed a resistant phenotype, whereas all of the european species were highly susceptible. the alachua and conquistador accessions of vitis rotundifolia species, which originated in north america, were immune to the disease, whil ...	2014	24854440
transcriptome of erysiphe necator-infected vitis pseudoreticulata leaves provides insight into grapevine resistance to powdery mildew.	powdery mildew (pm), which is caused by the pathogen erysiphe necator (schw.) burr., is the single most damaging disease of cultivated grapes (vitis vinifera) worldwide. however, little is known about the transcriptional response of grapes to infection with pm. rna-seq analysis was used for deep sequencing of the leaf transcriptome to study pm resistance in chinese wild grapes (v. pseudoreticulata baihe 35-1) to better understand the interaction between host and pathogen. greater than 100 millio ...	2014	26504551
can vessel dimension explain tolerance toward fungal vascular wilt diseases in woody plants? lessons from dutch elm disease and esca disease in grapevine.	this review illuminates key findings in our understanding of grapevine xylem resistance to fungal vascular wilt diseases. grapevine (vitis spp.) vascular diseases such as esca, botryosphaeria dieback, and eutypa dieback, are caused by a set of taxonomically unrelated ascomycete fungi. fungal colonization of the vascular system leads to a decline of the plant host because of a loss of the xylem function and subsequent decrease in hydraulic conductivity. fungal vascular pathogens use different col ...	2014	24971084
production, characterization of tannase from penicillium montanense urm 6286 under ssf using agroindustrial wastes, and application in the clarification of grape juice (vitis vinifera l.).	tannase is an enzyme that hydrolyzes esters and lateral bonds of tannins, such as tannic acid, releasing glucose and gallic acid and stands out in the clarification of wines and juices. fungi of the genera aspergillus and penicillium are excellent producers of this enzyme. the search for fungi that produce high levels of tannase as well as new substrates for the enzyme production by the ssf is required. the objectives of this study were to evaluate the production of tannase by aspergillus and pe ...	2014	25506607
exploring insights for virulent gene inhibition of multidrug resistant salmonella typhi, vibrio cholerae, and staphylococcus areus by potential phytoligands via in silico screening.	in our recent studies on prevalence of multidrug resistant pathogens in byramangala reservoir, karnataka, india, we identified salmonella typhi, staphylococcus aureus, and vibrio cholerae which had acquired multiple drug resistance (mdr) and emerged as superbugs. hence, there is a pressing demand to identify alternative therapeutic remedies. our study focused on the screening of herbal leads by structure-based virtual screening. the virulent gene products of these pathogens towards kanamycin(aph ...	2014	23876154
functional analysis of flavonoid 3',5'-hydroxylase from tea plant (camellia sinensis): critical role in the accumulation of catechins.	flavonoid 3',5'-hydroxylase (f3'5'h), an important branch point enzyme in tea plant flavan-3-ol synthesis, belongs to the cyp75a subfamily and catalyzes the conversion of flavones, flavanones, dihydroflavonols and flavonols into 3',4',5'-hydroxylated derivatives. however, whether b-ring hydroxylation occurs at the level of flavanones and/or dihydroflavonols, in vivo remains unknown.	2014	25490984
effects of distillation system and yeast strain on the aroma profile of albariño (vitis vinifera l.) grape pomace spirits.	orujo is a traditional alcoholic beverage produced in galicia (northwest spain) from distillation of grape pomace, a byproduct of the winemaking industry. in this study, the effect of the distillation system (copper charentais alembic versus packed column) and the yeast strain (native yeast l1 versus commercial yeast l2) on the chemical and sensory characteristics of orujo obtained from albariño (vitis vinifera l.) grape pomace has been analyzed. principal component analysis, with two components ...	2014	25307564
genome-wide identification and analysis of mitogen activated protein kinase kinase kinase gene family in grapevine (vitis vinifera).	mitogen-activated protein kinase kinase kinases (mapkkks; map3ks) are important components of mapk cascades, which are highly conserved signal transduction pathways in animals, yeast and plants, play important roles in plant growth and development. mapkkks have been investigated on their evolution and expression patterns in limited plants including arabidopsis, rice and maize.	2014	25158790
wild saccharomyces cerevisiae strains display biofilm-like morphology in contact with polyphenols from grapes and wine.	polyphenols are a major component of wine grapes, and contribute to color and flavor, but their influence upon yeast growth forms has not been investigated. in this work we have studied the effect of polyphenols on the ability of natural isolates of wine-related saccharomyces cerevisiae strains to form biofilms attaching to plastic surfaces, to grow as mat colonies, to invade media, and to display filamentous growth. the use of carbon- and nitrogen-rich or deficient media simulated grape juice f ...	2014	25150672
exploring three pips and three tips of grapevine for transport of water and atypical substrates through heterologous expression in aqy-null yeast.	aquaporins are membrane channels that facilitate the transport of water and other small molecules across the cellular membranes. we examined the role of six aquaporins of vitis vinifera (cv. touriga nacional) in the transport of water and atypical substrates (other than water) in an aqy-null strain of saccharomyces cerevisiae. their functional characterization for water transport was performed by stopped-flow fluorescence spectroscopy. the evaluation of permeability coefficients (pf) and activat ...	2014	25111598
selection of autochthonous saccharomyces cerevisiae strains as wine starters using a polyphasic approach and ochratoxin a removal.	over the last few years, the selection of autochthonous strains of saccharomyces cerevisiae as wine starters has been studied; however, researchers have not focused on the ability to remove ochratoxin a (ota) as a possible trait to use in oenological characterization. in this article, a polyphasic approach, including yeast genotyping, evaluation of phenotypic traits, and fermentative performance in a model system (temperature, 25 and 30°c; sugar level, 200 and 250 g liter(-1)), was proposed as a ...	2014	24988024
myb14, a direct activator of sts, is associated with resveratrol content variation in berry skin in two grape cultivars.	high and low resveratrol (res) contents in two cultivars are correlated with the expression abundance of myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( sts ). resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabolism in some plants. stilbene synthase (sts) is the key enzyme for the final step of precursor formation of resveratrol (res) in grapevines. in this study, we found that res contents in ripe be ...	2014	24948530
the grapevine tonoplast aquaporin tip2;1 is a pressure gated water channel.	in plants, the vacuole is a multifunctional organelle with an important role in the maintenance of the intracellular space. tonoplast membranes are highly permeable to water due to their content in aquaporins tips (tonoplast intrinsic proteins) that allow the rapid water influx creating an internal turgor pressure responsible for cell expansion, elongation and shape. the aim of the present study was to evaluate if the grapevine vitis vinifera tip2;1 would operate as a possible volume regulator g ...	2014	24942877
survival of the biological control agent candida sake cpa-1 on grapes under the influence of abiotic factors.	as reliability of preharvest applications of biological control agents (bcas) to control fruit pathogens is highly dependent on the survival of the selected organism, this study aimed to describe the population dynamics of the yeast-bca candida sake (saito & ota) cpa-1 on grape berries under the effect of abiotic factors such as temperature, relative humidity, sunlight and rainfall.	2014	24917056
the grapevine basic helix-loop-helix (bhlh) transcription factor positively modulates cbf-pathway and confers tolerance to cold-stress in arabidopsis.	basic helix-loop-helix (bhlh)-type transcription factors play diverse roles in plant physiological response and stress-adaptive regulation network. here, we identified one grapevine bhlh transcription factor from a cold-tolerant accession 'heilongjiang seedling' of chinese wild vitis amurensis (vabhlh1) as a transcriptional activator involved in cold stress. we also compared with its counterpart from a cold-sensitive vitis vinifera cv. cabernet sauvignon (vvbhlh1). these two putative proteins ar ...	2014	24859977
characterization of a grape class iv chitinase.	a chitinase was purified from vitis vinifera manzoni bianco grape juice and characterized. on the basis of proteomic analysis of tryptic peptides, a significant match identified the enzyme as a type iv grape chitinase previously found in juices of other v. vinifera varieties. the optimal ph and temperature for activity toward colloidal chitin were found to be 6 and 30 °c, respectively. the enzyme was found to hydrolyze chitin and oligomers of n-acetylglucosamine, generating n,n'-diacetylchitobio ...	2014	24845689
copper homeostasis in grapevine: functional characterization of the vitis vinifera copper transporter 1.	the vitis vinifera copper transporter 1 is capable of self-interaction and mediates intracellular copper transport. an understanding of copper homeostasis in grapevine (vitis vinifera l.) is particularly relevant to viticulture in which copper-based fungicides are intensively used. in the present study, the vitis vinifera copper transporter 1 (vvctr1), belonging to the ctr family of copper transporters, was cloned and functionally characterized. amino acid sequence analysis showed that vvctr1 mo ...	2014	24691572
hannaella pagnoccae sp. nov., a tremellaceous yeast species isolated from plants and soil.	several independent surveys of yeasts associated with different plant materials and soil led to the proposal of a novel yeast species belonging to the tremellales clade (agaricomycotina, basidiomycota). analysis of the sequences of the d1/d2 domains and internal transcribed spacer region of the large subunit of the rrna gene suggested affinity to a phylogenetic lineage that includes hannaella coprosmaensis, hannaella oryzae and hannaella sinensis. thirty-two isolates were obtained from different ...	2014	24614844
effects of vine water status on dimethyl sulfur potential, ammonium, and amino acid contents in grenache noir grapes (vitis vinifera).	we studied the effect of vine water status on the dimethyl sulfur potential (dmsp), ammonium, and amino acid contents of the berry during the maturation of grenache noir grapes. water deficit increased the accumulation of amino acids in berries and favored yeast assimilable amino nitrogen. similarly, ammonium content was higher in berries from vines subjected to moderate water deficit. dmsp content followed the same trend as yeast assimilable amino acid content, with higher concentrations observ ...	2014	24611597
it's time to pop a cork on champagne's proteome!	champagne is a world-renowned french sparkling wine, which undergoes many steps (fermentation, aging …) for its elaboration. various compounds might evolve during this winemaking process and thus modify its final quality. here, we report the first proteome analysis of two standard commercial champagne wines, using the powerful combinatorial peptide ligand library (cpll) technique. indeed, wine proteins are present in small amounts but they are key compounds, likely to impact on both foam quality ...	2014	24594285
biodiversity study of wine yeasts belonging to the "terroir" of montepulciano d'abruzzo "colline teramane" revealed saccharomyces cerevisiae strains exhibiting atypical and unique 5.8s-its restriction patterns.	the montepulciano d'abruzzo "colline teramane" premium wine docg is produced in the teramo province (abruzzo, italy). this region has a great tradition in winemaking and the wine is produced by a spontaneous fermentation so it could represent a reservoir of wine natural yeasts with important oenological features. the aim of this study was to characterize the yeast community of this wine grape growing region in order to create a saccharomyces cerevisiae bank, providing data on oenological propert ...	2014	24387846
the grape aquaporin vvsip1 transports water across the er membrane.	water diffusion through biological membranes is facilitated by aquaporins, members of the widespread major intrinsic proteins (mips). in the present study, the localization, expression, and functional characterization of a small basic intrinsic protein (sip) from the grapevine were assessed. vvsip1 was expressed in leaves and berries from field-grown vines, and in leaves and stems from in vitro plantlets, but not in roots. when expressed in tobacco mesophyll cells and in saccharomyces cerevisiae ...	2014	24376256
influence of new generation fungicides on saccharomyces cerevisiae growth, grape must fermentation and aroma biosynthesis.	the influence of ten new generation fungicides (ametoctradin, benthiavalicarb-isopropyl, boscalid, cyazofamid, dimethomorph, fenhexamid, kresoxim-methyl, mepanipyrim, metrafenone, and pyraclostrobin) on the fermentative activity of saccharomyces cerevisiae yeast was initially evaluated in pasteurised red must. the presence of ametoctradin, dimethomorph and mepanipyrim seemed to affect sugars-to-ethanol yield in the stationary phase. the same fermentation experiments were carried out for these th ...	2014	24176337
effect of temperature on symptom expression and sequence polymorphism of grapevine yellow speckle viroid 1 in grapevine.	symptom expression of yellow speckle disease was studied in a row of 32 individual vitis vinifera cv. chardonnay vines in the warmer summer of 2009/2010 as compared with the cooler summer of 2011/2012 in south australia. rt-pcr analysis showed that all these vines were positive for hop stunt viroid, grapevine yellow speckle viroid 1 (gysvd-1) and grapevine rupestris stem pitting-associated virus. four vines named vines 1, 8, 11 and 15 were selected for further analyses. vines 1 and 8 had never s ...	2014	24905287
spatial distribution of plant-parasitic nematodes in semi-arid vitis vinifera vineyards in washington.	the most commonly encountered plant-parasitic nematodes in eastern washington vitis vinifera vineyards are meloidogyne hapla, mesocriconema xenoplax, pratylenchus spp., xiphinema americanum, and paratylenchus sp.; however, little is known about their distribution in the soil profile. the vertical and horizontal spatial distribution of plant-parasitic nematodes was determined in two washington v. vinifera vineyards. others variables measured in these vineyards included soil moisture content, fine ...	2014	25580024
genetic diversity of grapevine virus a in washington and california vineyards.	grapevine virus a (gva; genus vitivirus, family betaflexiviridae) has been implicated with the kober stem grooving disorder of the rugose wood disease complex. in this study, 26 isolates of gva recovered from wine grape (vitis vinifera) cultivars from california and washington were analyzed for their genetic diversity. an analysis of a portion of the rna-dependent rna polymerase (rdrp) and complete coat protein (cp) sequences revealed intra- and inter-isolate sequence diversity. our results indi ...	2014	24168043
antifungal activity of resveratrol derivatives against candida species.	trans-resveratrol (1a) is a phytoalexin produced by plants in response to infections by pathogens. its potential activity against clinically relevant opportunistic fungal pathogens has previously been poorly investigated. evaluated herein are the candidacidal activities of oligomers (2a, 3-5) of 1a purified from vitis vinifera grape canes and several analogues (1b-1j) of 1a obtained through semisynthesis using methylation and acetylation. moreover, trans-ε-viniferin (2a), a dimer of 1a, was also ...	2014	25014026
evaluation of anti-candida activity of vitis vinifera l. seed extracts obtained from wine and table cultivars.	for the first time, grape seed extracts (gses), obtained from wine and table cultivars of vitis vinifera l., cultured in experimental fields of lazio and puglia regions of italy and grown in different agronomic conditions, have been tested on 43 candida species strains. we demonstrated a significant correlation between the content of the flavan-3-ols in gses extracts, with a polymerization degree ≥ 4, and anti-candida activity. moreover, we demonstrated that gses, obtained from plants cultured w ...	2014	24864227
interkingdom transfer of the acne-causing agent, propionibacterium acnes, from human to grapevine.	here, we report the surprising and, to our knowledge, unique example of horizontal interkingdom transfer of a human opportunistic pathogen (propionibacterium acnes) to a crop plant (the domesticated grapevine vitis vinifera l.). humans, like most organisms, have established a long-lasting cohabitation with a variety of microbes, including pathogens and gut-associated bacteria. studies which have investigated the dynamics of such associations revealed numerous cases of bacterial host switches fro ...	2014	24554779
[comparison of stilbene synthase from different plant sources for resveratrol biosynthesis].	resveratrol is a natural phytoalexin with special pharmacological and health functions. stilbene synthase (sts) is a key and rate-limiting enzyme in the biosynthesis of resveratrol that is present only in a limited number of plants. the content of resveratrol from polygonum cuspidatum is more than 1000 times higher than grapes and peanuts. we speculate that the catalytic ability of different sts may be one of the reasons causing differences in the content of resveratrol. to verify the above spec ...	2014	25726587
extraction process optimization of polyphenols from indian citrus sinensis - as novel antiglycative agents in the management of diabetes mellitus.	diabetes mellitus is a chronic metabolic disorder characterized by increased blood glucose level. it has become an epidemic disease in the 21st century where, india leads the world with largest number of diabetic subjects. non-enzymatic glycosylation (glycation) is severe form of diabetes, occurs between reducing sugar and proteins which results in the formation of advanced glycation end products (ages) that leads to the other complicated secondary disorders. in this context, mangifera indica (m ...	2014	24397983
foraging leaf-cutting ants learn to reject vitis vinifera ssp. vinifera plants that emit herbivore-induced volatiles.	leaf-cutting ants (lcas) are dominant herbivores of the neotropics, as well as economically important pests. their foraging ecology and patterns/mechanisms of food selection have received considerable attention. recently, it has been documented that lcas exhibit a delayed rejection of previously accepted food plants following treatment with a fungicide that makes the plants unsuitable as substrate for their symbiotic fungus. here, we investigated whether lcas similarly reject plants with induced ...	2014	24929941
members of the npf3 transporter subfamily encode pathogen-inducible nitrate/nitrite transporters in grapevine and arabidopsis.	vitis vinifera, the major grapevine species cultivated for wine production, is very susceptible to erysiphe necator, the causal agent of powdery mildew (pm). this obligate biotrophic fungal pathogen attacks both leaf and berry, greatly affecting yield and quality. to investigate possible mechanisms of nutrient acquisition by successful biotrophs, we characterized a candidate nitrate transporter1/peptide transporter family (npf, formerly nrt1/ptr) member, grapevine nfp3.2, that was up-regulated i ...	2014	24259683
initial description of the developing soybean seed protein lys-n(ε)-acetylome.	characterization of the myriad protein posttranslational modifications (ptm) is a key aspect of proteome profiling. while there have been previous studies of the developing soybean seed phospho-proteome, herein we present the first analysis of non-histone lysine-n(ɛ)-acetylation in this system. in recent years there have been reports that lysine acetylation is widespread, affecting thousands of proteins in diverse species from bacteria to mammals. recently preliminary descriptions of the protein ...	2014	24211405
comparative analysis of predicted plastid-targeted proteomes of sequenced higher plant genomes.	plastids are actively involved in numerous plant processes critical to growth, development and adaptation. they play a primary role in photosynthesis, pigment and monoterpene synthesis, gravity sensing, starch and fatty acid synthesis, as well as oil, and protein storage. we applied two complementary methods to analyze the recently published apple genome (malus × domestica) to identify putative plastid-targeted proteins, the first using targetp and the second using a custom workflow utilizing a ...	2014	25393533
diadenosine triphosphate is a novel factor which in combination with cyclodextrins synergistically enhances the biosynthesis of trans-resveratrol in vitis vinifera cv. monastrell suspension cultured cells.	dinucleoside polyphosphates are considered as signal molecules that may evoke response of plant cells to stress. other compounds whose biological effects have been recognized are cyclodextrins. they are cyclic oligosaccharides that chemically resemble the alkyl-derived pectic oligosaccharides naturally released from the cell walls during fungal attack, and they act as true elicitors, since, when added to plant cell culture, they induce the expression of genes involved in some secondary metabolis ...	2014	25310254
begin at the beginning: a bac-end view of the passion fruit (passiflora) genome.	the passion fruit (passiflora edulis) is a tropical crop of economic importance both for juice production and consumption as fresh fruit. the juice is also used in concentrate blends that are consumed worldwide. however, very little is known about the genome of the species. therefore, improving our understanding of passion fruit genomics is essential and to some degree a pre-requisite if its genetic resources are to be used more efficiently. in this study, we have constructed a large-insert bac ...	2014	25260959
integrated syntenic and phylogenomic analyses reveal an ancient genome duplication in monocots.	unraveling widespread polyploidy events throughout plant evolution is a necessity for inferring the impacts of whole-genome duplication (wgd) on speciation, functional innovations, and to guide identification of true orthologs in divergent taxa. here, we employed an integrated syntenic and phylogenomic analyses to reveal an ancient wgd that shaped the genomes of all commelinid monocots, including grasses, bromeliads, bananas (musa acuminata), ginger, palms, and other plants of fundamental, agric ...	2014	25082857
activity-based profiling of a physiologic aglycone library reveals sugar acceptor promiscuity of family 1 udp-glucosyltransferases from grape.	monoterpenols serve various biological functions and accumulate in grape (vitis vinifera), where a major fraction occurs as nonvolatile glycosides. we have screened the grape genome for sequences with similarity to terpene uridine diphosphate glycosyltransferases (ugts) from arabidopsis (arabidopsis thaliana). a ripening-related expression pattern was shown for three candidates by spatial and temporal expression analyses in five grape cultivars. transcript accumulation correlated with the produc ...	2014	25073706
plant microrna-target interaction identification model based on the integration of prediction tools and support vector machine.	confident identification of microrna-target interactions is significant for studying the function of microrna (mirna). although some computational mirna target prediction methods have been proposed for plants, results of various methods tend to be inconsistent and usually lead to more false positive. to address these issues, we developed an integrated model for identifying plant mirna-target interactions.	2014	25051153
a udp-glucose:monoterpenol glucosyltransferase adds to the chemical diversity of the grapevine metabolome.	terpenoids represent one of the major classes of natural products and serve different biological functions. in grape (vitis vinifera), a large fraction of these compounds is present as nonvolatile terpene glycosides. we have extracted putative glycosyltransferase (gt) sequences from the grape genome database that show similarity to arabidopsis (arabidopsis thaliana) gts whose encoded proteins glucosylate a diversity of terpenes. spatial and temporal expression levels of the potential vvgt genes ...	2014	24784757
study on folate binding domain of dihydrofolate reductase in different plant species and human beings.	data base (ncbi and tigr) searches are made to retrieve protein sequences of different plant species namely medicago truncatula, pisum sativum, ricinus communis, arabidopsis thaliana, vitis vinifera, glycine max, daucus carota, oryza sativa japonica group, arabidopsis lyrata subsp. lyrata, brachypodium distachyon, oryza sativa indica group, zea mays and careful alignment of derived sequences shows 95% or higher identity. similarly, dhfr sequence of human being is also retrieved from ncbi. a phyl ...	2014	24616563
genomic analysis of nac transcription factors in banana (musa acuminata) and definition of nac orthologous groups for monocots and dicots.	identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. a comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. nac transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. the main goal of this study was to set up a framework of orthologous groups determined by an expert sequence compar ...	2014	24570169
an update on sugar transport and signalling in grapevine.	in addition to their role as a source of reduced carbon, sugars may directly or indirectly control a wide range of activities in plant cells, through transcriptional and post-translational regulation. this control has been studied in detail using arabidopsis thaliana, where genetic analysis offers many possibilities. much less is known about perennial woody species. for several years, various aspects of sugar sensing and signalling have been investigated in the grape (vitis vinifera l.) berry, a ...	2014	24323501
rapid and multiregional adaptation to host partial resistance in a plant pathogenic oomycete: evidence from european populations of plasmopara viticola, the causal agent of grapevine downy mildew.	crop pathogens evolve rapidly to adapt to their hosts. the use of crops with quantitative disease resistance is expected to alter selection of pathogen life-history traits. this may result in differential adaptation of the pathogen to host cultivars and, sometimes, to the erosion of quantitative resistance. here, we assessed the level of host adaptation in an oomycete plant pathogenic species. we analysed the phenotypic and genetic variability of 17 plasmopara viticola isolates collected on viti ...	2014	24184095
actin marker lines in grapevine reveal a gatekeeper function of guard cells.	resistance to abiotic and biotic stress is a central topic for sustainable agriculture, especially in grapevine, one of the field crops with the highest economic output per acreage. as early cellular factors for plant defense, actin microfilaments (af) are of high relevance. we therefore generated a transgenic actin marker line for grapevine by expressing a fusion protein between green fluorescent protein and the second actin-binding domain of arabidopsis (arabidopsis thaliana) fimbrin, atfim1. ...	2014	24973589
the sulfated laminarin triggers a stress transcriptome before priming the sa- and ros-dependent defenses during grapevine's induced resistance against plasmopara viticola.	grapevine (vitis vinifera) is susceptible to many pathogens which cause significant losses to viticulture worldwide. chemical control is available, but agro-ecological concerns have raised interest in alternative methods, especially in triggering plant immunity by elicitor treatments. the β-glucan laminarin (lam) and its sulfated derivative (ps3) have been previously demonstrated to induce resistance in grapevine against downy mildew (plasmopara viticola). however, if lam elicits classical grape ...	2014	24516597
soybean and casein hydrolysates induce grapevine immune responses and resistance against plasmopara viticola.	plasmopara viticola, the causal agent of grapevine downy mildew, is one of the most devastating grape pathogen in europe and north america. although phytochemicals are used to control pathogen infections, the appearance of resistant strains and the concern for possible adverse effects on environment and human health are increasing the search for alternative strategies. in the present investigation, we successfully tested two protein hydrolysates from soybean (soy) and casein (cas) to trigger gra ...	2014	25566290
simultaneous quantification of sporangia and zoospores in a biotrophic oomycete with an automatic particle analyzer: disentangling dispersal and infection potentials.	quantitative pathogenicity traits drive the fitness and dynamics of pathogens in agricultural ecosystems and are key determinants of the correct management of crop production over time. however, traits relating to infection potential (i.e. zoospore production) have been less thoroughly investigated in oomycetes than traits relating to dispersal (i.e. sporangium production). we simultaneously quantified sporangium and zoospore production in a biotrophic oomycete, for the joint assessment of life- ...	2014	25448022
genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using rapd.	in vitro cultured shoot-tips of four grape (vitis vinifera) cultivars and one kiwi (actinidia deliciosa) cultivar were cryopreserved using the encapsulation-dehydration method. genomic dna of plantlets regenerated directly from cryopreserved shoot-tips was extracted and analyzed using the rapd (random amplified polymorphic dna) technique. the rapd profiles obtained were highly reproducible and no differences were found between the dna patterns obtained with plantlets regenerated from control and ...	2014	14566391
immunomodulating and anti-allergic effects of negroamaro and koshu vitis vinifera fermented grape marc (fgm).	polyphenols contained in fgm from negroamaro (n) and koshu (k) vitis vinifera have been shown to exhibit several immunomodulating activities. for instance, mice affected by experimental colitis when administered with k-fgm showed an attenuation of the inflammatory process. in murine asthma, k-fgm reduced ige production and eosinophil number in bronchial alveolar lavage fluid. in vitro, both n- and k-fgm were able to induce t regulatory cells in terms of foxp-3 molecule expression and release of ...	2014	23701568