Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| case-control study on risk factors associated with brucella melitensis in goat farms in peninsular malaysia. | caprine brucellosis is a bacterial zoonotic infection affecting goats especially in developing countries all over the world. in malaysia, the risk factors associated with this infection in farms have not been studied. a case-control study was carried out in goat farms in four states of malaysia to elucidate the risk factors associated with the infection on the farms using structured questionnaires and face-to-face interviews. results indicate that the introduction of new animals (or = 5.25; 90 % ... | 2014 | 24633657 |
| [two spondylodiscitis due to a contamination by brucella melitensis in a mother and daughter back from holidays]. | we describe here a case of spondylodiscitis of brucellosis origin in one patient back from turkey. some weeks later, her mother, who had accompagnied her in turkey also developped similar symptoms. a diagnosis of spondylodiscitis due to a contamination by brucella melitensis was also proposed. since the control of animal brucellosis in europe, human cases are rarer. sporadic cases still observed are mostly travellers back from aerea where animal brucellosis remains endemic. seroprevalence of a s ... | 2014 | 24624738 |
| validation of a simple universal ielisa for the diagnosis of human brucellosis. | the definitive diagnosis of brucellosis requires isolation of the agent, although negative isolation does not rule out the infection. in contrast, serological testing is more sensitive and, therefore, preferred in clinical practice. the majority of reported cases around the world were caused by brucella melitensis, b. abortus, b. suis and b. canis. the first three species contain o-polysaccharide (ops) on the cell surface, but b. canis contains no measurable ops on the rough lipopolysaccharide ( ... | 2014 | 24515098 |
| assessment of genetic diversity of zoonotic brucella spp. recovered from livestock in egypt using multiple locus vntr analysis. | brucellosis is endemic in most parts of egypt, where it is caused mainly by brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. knowledge of the predominant brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. for this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 brucella spp. isolates recovered from domestic ruminants (catt ... | 2014 | 24511531 |
| evaluation of the immunogenicity and safety of brucella melitensis b115 vaccination in pregnant sheep. | in spite of its limitations, rev.1 is currently recognized as the most suitable vaccine against brucella melitensis (the causative agent of ovine and caprine brucellosis). however, its use is limited to young animals when test-and-slaughter programs are in place because of the occurrence of false positive-reactions due to rev.1 vaccination. the b. melitensis b115 rough strain has demonstrated its efficacy against b. melitensis virulent strains in the mouse model, but there is a lack of informati ... | 2014 | 24508034 |
| a pregnant mouse model for the vertical transmission of brucella melitensis. | abortion is the major clinical sign of brucellosis in animals but little is known about the underlying mechanisms. this study was designed to evaluate a pregnant mouse model for the vertical transmission of brucella melitensis using four infectious doses: 10(3) colony-forming units (cfu), 10(4) cfu, 10(5) cfu, and 10(6) cfu. during the experimental period, no instances of abortion were recorded, but stillbirths were observed in the groups infected with doses of 10(4) cfu and higher. regardless o ... | 2014 | 24462801 |
| brucella melitensis infection of ventriculo-peritoneal shunt: a form of neurobrucellosis manifested as gastrointestinal symptoms. | a report of a 9 year-old child with myelomeningocele who has a ventriculo-peritoneal (vp) shunt presented with gastrointestinal symptoms and peritonitis. the patient had no cns symptoms but the cerebrospinal fluid was positive for brucella melitensis. we discuss neurobrucellosis in children, its various presentations, complications and challenges in treatment, choice of antibiotics and duration. | 2014 | 23999355 |
| brucellosis as an aetiology of septic arthritis. | brucellosis is a zoonotic disease caused by a gram-negative coccobacillus from the brucella genus. the disease has a broad spectrum of clinical manifestations. the musculoskeletal system involvement is frequent and, rarely, arthritis can be the only clinical feature of the disease. we report a case of monoarthritis caused by brucella melitensis. | 2014 | 24231687 |
| spinal brucellosis in south of tunisia: review of 32 cases. | brucellosis remains an important economic and public health problem in some parts of the world. the spine is the most common site of musculoskeletal involvement of brucellosis. | 2014 | 24331843 |
| a brucellosis disease control strategy for the kakheti region of the country of georgia: an agent-based model. | brucellosis has been reported in livestock and humans in the country of georgia with brucella melitensis as the most common species causing disease. georgia lacked sufficient data to assess effectiveness of the various potential control measures utilizing a reliable population-based simulation model of animal-to-human transmission of this infection. therefore, an agent-based model was built using data from previous studies to evaluate the effect of an animal-level infection control programme on ... | 2014 | 23879523 |
| camelid brucellosis: a review. | camel brucellosis has been diagnosed in all camel-rearing countries except australia. in many countries the infection is on the rise in old world camels (owcs) due to the uncontrolled trade of live animals. knowledge of camelid brucellosis has increased over the last decade through field investigations, experimental infection trials and comprehensive laboratory testing. infection with brucella melitensis is frequent in owcs and rare with b. abortus. new world camels rarely contract brucellosis. ... | 2014 | 25812208 |
| [study on the epidemiologic characteristic of brucella melitensis isolated in guizhou province in 2010-2012]. | to understand the genetic and epidemiologic characteristic of brucella (b.) melitensis strains isolated in guizhou province in 2010-2012. | 2014 | 25567021 |
| melitococcosis in the republic of croatia. | melitococcosis is one of the most widespread zoonoses worldwide. in the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the republic of croatia. | 2014 | 25536995 |
| clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. | brucellosis is an important disease in developing countries. we aimed to determine the epidemiologic, clinical, and laboratory characteristics of brucellosis, which still has a high morbidity in turkey. | 2014 | 25536728 |
| human brucellosis in maghreb: existence of a lineage related to socio-historical connections with europe. | despite control/eradication programs, brucellosis, major worldwide zoonosis due to the brucella genus, is endemic in northern africa and remains a major public health problem in the maghreb region (algeria/morocco/tunisia). brucella melitensis biovar 3 is mostly involved in human infections and infects mainly small ruminants. human and animal brucellosis occurrence in the maghreb seems still underestimated and its epidemiological situation remains hazy. this study summarizes official data, regar ... | 2014 | 25517901 |
| first case report of brucellosis in a child in thailand. | brucellosis is uncommon in children. in thailand, there have been only seven adult cases reported, all with brucella melitensis. we describe here the first reported pediatric case of brucellosis in thailand. a 12-year old boy presented with prolonged fever for one month, pancytopenia, pneumonia and peritonitis. the blood culture grew out brucella melitensis. he responded well to combination therapy consisting of doxycycline and gentamicin. he recovered fully without relapse during the 6 month fo ... | 2014 | 25507608 |
| first case report of brucellosis in a child in thailand. | brucellosis is uncommon in children. in thailand, there have been only seven adult cases reported, all with brucella melitensis. we describe here the first reported pediatric case of brucellosis in thailand. a 12-year old boy presented with prolonged fever for one month, pancytopenia, pneumonia and peritonitis. the blood culture grew out brucella melitensis. he responded well to combination therapy consisting of doxycycline and gentamicin. he recovered fully without relapse during the 6 month fo ... | 2014 | 25427358 |
| immunization of mice with a novel recombinant molecular chaperon confers protection against brucella melitensis infection. | brucella spp. are zoonotic gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. it has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. bmei1549 is a molecular chaperone designated dnak that is expressed under stress conditions and helps to prevent formation of protein aggregates. in order to study the potential of dnak as a prospective brucella subunit vac ... | 2014 | 25240754 |
| renal abscess caused by brucella. | involvement of the renal parenchyma in the acute phase of brucellosis is very rare. only two cases of renal brucelloma have been reported in the english language literature to date. we report a case of renal abscess caused by brucella in the acute phase. a 45-year-old chinese man presented with a high fever, urine occult blood, and a low density lesion in the right kidney. ultrasound-guided aspiration was done. brucella melitensis was isolated from both blood and puncture fluid culture. minocycl ... | 2014 | 25220238 |
| sirna screens using drosophila cells to identify host factors required for infection. | drosophila melanogaster offers a powerful model system for interrogating interactions between host cells and human bacterial pathogens. brucella, a gram-negative, facultative intracellular bacterium is the causative agent of brucellosis, a zoonotic disease of global consequence. over the past several decades, pathogen factors that mediate brucella infection have been identified. however, host factors that mediate infection have remained obscure. we have used the power of the drosophila s2 cell s ... | 2014 | 25172284 |
| studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging. | despite progress in mouse models of bacterial pathogens, studies are often limited by evaluating infections in an individual organ or tissue or at a given time. here we present a technique to engineer the pathogen, e.g., brucella melitensis, with a bioluminescent marker permitting analysis of living bacteria in real time during the infectious process from acute to chronic infection. using this bioluminescent approach, tissue preference, differences between virulent and mutant bacteria, as well a ... | 2014 | 25172275 |
| galleria mellonella as an infection model for select agents. | the use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. to avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. one of these models, the larvae of the greater wax moth galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. here, we describe the use g. mellonella to study virulence of the hig ... | 2014 | 25172272 |
| reemergence of brucella melitensis in wildlife, france. | 2014 | 25152274 | |
| liver histology of acute brucellosis caused by brucella melitensis. | as a major organ of the mononuclear phagocytic system, the liver is probably involved in all cases of brucellosis. in this prospective study, liver slides prepared from percutaneous liver biopsy samples of 20 patients with clinical and laboratory evidence of acute brucellosis due to brucella melitensis were examined for the presence or absence of granulomas by pathologists in iran and the united states. nineteen men and one woman ranging in age from 14 to 62 years were studied. all patients had ... | 2014 | 25147098 |
| [distribution of potential pathogenic bacteria in the jiulong river watershed]. | recently, the human activities including economic growth and urbanization posed serious environmental health risks to the jiulong river watershed (jrw). in order to gain a full understanding of the distribution of potential pathogenic bacteria (ppb) in this area, we used 16s rrna amplicon pyrosequencing technology to investigate planktonic and benthic bacterial community in two main tributaries (north river, nr, and west river, wr) of the jiulong river (jr). at the genus level, a total of 68 gen ... | 2014 | 25055661 |
| evaluation of a brucella melitensis mutant deficient in o-polysaccharide export system atp-binding protein as a rough vaccine candidate. | rough brucella mutants have been sought as vaccine candidates that do not interfere with the conventional serological diagnosis of brucellosis. in this study, a rough mutant of brucella melitensis was generated by the disruption of the wzt gene, which encodes the o-polysaccharide (o-ps) export system atp-binding protein. in vivo, the mutant 16mδwzt was attenuated and conferred a level of protection against b. melitensis 16m challenge similar to that conferred by the vaccine strain b. melitensis ... | 2014 | 25043564 |
| identification of a new immunogenic candidate conferring protection against brucella melitensis infection in mice. | identification of bacterial proteins that contribute to the replication and survival of the engulfed bacteria within phagolysosome is critical in the pathogenesis of intracellular bacteria. heat shock proteins (hsps) are molecular chaperones that prevent unwanted protein aggregation and protect the bacteria against cell stress. in order to study the potential of hspa for development of a brucella subunit vaccine, immunogenicity and protective efficacy of recombinant hspa (rhspa) from brucella me ... | 2014 | 24995396 |
| a functional and phylogenetic comparison of quorum sensing related genes in brucella melitensis 16m. | a quorum-sensing (qs) system is involved in brucella melitensis survival inside the host cell. two transcriptional regulators identified in b. melitensis, blxr and vjbr, regulate the expression of virb, an operon required for bacterial intracellular persistence. in this work, 628 genes affected by vjbr and 124 by blxr were analyzed to gain insights into their functional and taxonomical distributions among the bacteria and archaea cellular domains. in this regard, the cluster of orthologous group ... | 2014 | 24994008 |
| main functions and taxonomic distribution of virulence genes in brucella melitensis 16 m. | many virulence genes have been detected in attenuated mutants of brucella melitensis 16 m; nevertheless, a complete report of these genes, including the main cluster of orthologous groups (cog) represented as well as the taxonomical distribution among all complete bacterial and archaeal genomes, has not been analyzed. in this work a total of 160 virulence genes that have been reported in attenuated mutants in b. melitensis were included and analyzed. additionally, we obtained 250 b. melitensis r ... | 2014 | 24964015 |
| septic arthritis caused by brucella melitensis in urban shenzhen, china: a case report. | brucellosis is a systemic infectious disease which is still a challenging medical problem in rural areas such as northern china. it rarely occurs in urban areas such as shenzhen in southern china. osteoarticular involvements are frequently seen in brucellosis, and rarely is arthritis the only clinical presentation. we report a case of hip septic arthritis caused by brucella melitensis in an urban area of shenzhen, china. | 2014 | 25394500 |
| isolated severe immune thrombocytopenia due to acute brucellosis. | mild anemia and leukopenia are the most common hematologic findings in the course of acute brucellosis. however severe form of thrombocytopenia is less frequently reported. the patient was admitted to the hospital with fever, gingival bleeding, and petechial skin lesions related to severe thrombocytopenia. he was investigated for the causes of thrombocytopenia. test results showed that wright agglutination test was positive at 1/5120 titer, and blood culture was positive for brucella melitensis. ... | 2014 | 25332526 |
| [clinical, serological and polymerase chain reaction follow-up of a family with brucellosis]. | human brucellosis diagnosis is based on isolation of brucella spp. from blood or tissue cultures with a positivity rate of 40-70% and serology techniques are used as complementary tools; recently molecular biology diagnostic techniques have been developed intending to optimize the etiological confirmation. | 2014 | 25327196 |
| detection of brucella melitensis in bovine milk and milk products from apparently healthy animals in egypt by real-time pcr. | brucellosis in egypt is an endemic disease among animals and humans. in endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. the aim of this study was to detect brucellae in milk and milk products produced from apparently healthy animals to estimate the prevalence of contamination. | 2014 | 25313613 |
| brucella melitensis sternal osteomyelitis following median sternotomy. | human brucellosis, a zoonotic infection, may present with a range of symptoms but is rarely described as a cause of surgical site infections. we present the first reported case of brucella melitensis causing sternal osteomyelitis of a midline sternotomy for a coronary artery bypass graft. the operation was performed in a non-endemic country but the patient had travelled to syria immediately before surgery, where the infection was assumed to have been acquired. the infection resolved following tr ... | 2014 | 24910374 |
| draft genome sequence of the field isolate brucella melitensis strain bm ind1 from india. | brucella spp. are facultative intracellular bacterial pathogens causing the zoonotic disease brucellosis. here, we report the draft genome sequence of the brucella melitensis strain from india designated bm ind1, isolated from stomach contents of an aborted goat fetus. | 2014 | 24874680 |
| the cyclic amp-binding protein cbpb in brucella melitensis and its role in cell envelope integrity, resistance to detergent and virulence. | brucella melitensis possesses an operon with two components: the response regulator otpr and a putative camp-dependent protein kinase regulatory subunit encoded by the bmei0067 gene. in the previous study, the function of otpr has been studied, while little is known about the function of the bmei0067 gene. using a bioinformatics approach, we showed that the bmei0067 gene encodes an additional putative camp-binding protein, which we refer to as cbpb. structural modeling predicted that cbpb has a ... | 2014 | 24850100 |
| in silico analysis of chimeric tf, omp31 and bp26 fragments of brucella melitensis for development of a multi subunit vaccine candidate. | brucellosis, especially caused by brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. the commonly used live attenuated vaccine in ovine brucellosis prophylaxis is b. melitensis rev1. but due to different problems caused by the administration of this vaccine, a protective subunit vaccine against b. melitensis is strongly demanded. brucella bp26, omp31 and tf proteins have shown a considerable potential as protectiv ... | 2014 | 24847419 |
| interferon-gamma responses in sheep exposed to virulent and attenuated brucella melitensis strains. | antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. in contrast, information on the cellular mediated immune (cmi) response triggered after brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced cmi reaction. here, the interferon-gamma (ifn-γ) profiles evoked after exposure by different routes ... | 2014 | 24814244 |
| thermostable cross-protective subunit vaccine against brucella species. | a subunit vaccine candidate was produced from brucella suis 145 (biovar 4; expressing both the a antigen of brucella abortus and the m antigen of brucella melitensis). the preparation consisted mostly of polysaccharide (ps; >90% [wt/wt]; both cell-associated ps and exo-ps were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. vaccinated mice were protected (these had a statistically significant reduction in bacterial colonization compared to that of unvaccinated c ... | 2014 | 25320267 |
| replication of brucella abortus and brucella melitensis in fibroblasts does not require atg5-dependent macroautophagy. | several intracellular bacterial pathogens have evolved subtle strategies to subvert vesicular trafficking pathways of their host cells to avoid killing and to replicate inside the cells. brucellae are gram-negative facultative intracellular bacteria that are responsible for brucellosis, a worldwide extended chronic zoonosis. following invasion, brucella abortus is found in a vacuole that interacts first with various endosomal compartments and then with endoplasmic reticulum sub-compartments. bru ... | 2014 | 25179110 |
| brucella melitensis invades murine erythrocytes during infection. | brucella spp. are facultative intracellular gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. we observed that brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. bacterial persistence in the blood is dramatically impaired by specific antibodies induced following brucella vaccination. in contrast to bartonella, t ... | 2014 | 25001604 |
| molecular detection of brucella species in patients suspicious of brucellosis from zanjan, iran. | brucella is an intracellular pathogen capable of infecting animals and humans. the aim of this study was to identify brucella spp in sera of high risk individuals by a polymerase chain reaction (pcr)-based method. a total of 180 patients suspected to have brucellosis were examined by serological tests. to establish a pcr protocol for diagnosis of active brucellosis, dna was extracted from the serum samples by using a commercial kit. pcr amplification was done for detection of brocella dna using ... | 2014 | 25242938 |
| identification and in vitro antimicrobial susceptibility of brucella species isolated from human brucellosis. | brucellosis is a world-wide zoonotic disease with a major impact on the public health. due to the high risk of laboratory acquired infection, limited laboratory investigations were performed on this organism, including detailed identification and susceptibility study. brucella melitensis is the commonest aetiological agent for human brucellosis in this region. the in vitro susceptibility pattern against selected antimicrobial agents was assessed using e-test. all isolates were noted to be sensit ... | 2014 | 25120569 |
| a protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. | human brucellosis is most commonly diagnosed by serology based on agglutination of fixed brucella abortus as antigen. nucleic acid amplification techniques have not proven capable of reproducibly and sensitively demonstrating the presence of brucella dna in clinical specimens. we sought to optimize a monoclonal antibody-based assay to detect brucella melitensis lipopolysaccharide in blood by conjugating b. melitensis lps to keyhole limpet hemocyanin, an immunogenic protein carrier to maximize ig ... | 2014 | 24901521 |
| brucella melitensis in france: persistence in wildlife and probable spillover from alpine ibex to domestic animals. | bovine brucellosis is a major zoonosis, mainly caused by brucella abortus, more rarely by brucella melitensis. france has been bovine brucellosis officially-free since 2005 with no cases reported in domestic/wild ruminants since 2003. in 2012, bovine and autochthonous human cases due to b. melitensis biovar 3 (bmel3) occurred in the french alps. epidemiological investigations implemented in wild and domestic ruminants evidenced a high seroprevalence (>45%) in alpine ibex (capra ibex); no cases w ... | 2014 | 24732322 |
| proteomic analysis of detergent resistant membrane domains during early interaction of macrophages with rough and smooth brucella melitensis. | the plasma membrane contains discrete nanometer-sized domains that are resistant to non-ionic detergents, and which are called detergent resistant membrane domains (drmds) or lipid rafts. exposure of host cells to pathogenic bacteria has been shown to induce the re-distribution of specific host proteins between drmds and detergent soluble membranes, which leads to the initiation of cell signaling that enable pathogens to access host cells. drmds have been shown to play a role in the invasion of ... | 2014 | 24643124 |
| shedding rates and seroprevalence of brucella melitensis in lactating goats of shahrekord, iran. | brucellosis remains a major worldwide zoonosis. caprine brucellosis is a significant problem for both public health and animal production. brucella melitensis causes disease in goats, sheep, humans, and occasionally cattle. transmission is by ingestion or contact with infected materials, vaginal discharge, or milk. | 2014 | 25147691 |
| collapsed l4 vertebral body caused by brucellosis. | brucellosis is caused by gram-negative, aerobic, non-motile, facultative, intracellular coccobacilli belonging to the genus brucella. a 50-year-old man working as an employee was admitted to neurosurgery clinic with severe low back, radicular right leg pain and hypoesthesia in right l4-5 dermatomes for 2 months. brucella tube agglutination (wright) test was positive in serum sample of the patient with a titer of 1/640. brucella melitensis was isolated from blood culture. x-ray and mri of the lom ... | 2014 | 24570819 |
| brucella melitensis t cell epitope recognition in humans with brucellosis in peru. | brucella melitensis, one of the causative agents of human brucellosis, causes acute, chronic, and relapsing infection. while t cell immunity in brucellosis has been extensively studied in mice, no recognized human t cell epitopes that might provide new approaches to classifying and prognosticating b. melitensis infection have ever been delineated. twenty-seven pools of 500 major histocompatibility complex class ii (mhc-ii) restricted peptides were created by computational prediction of promiscuo ... | 2014 | 24126518 |
| recovery of a medieval brucella melitensis genome using shotgun metagenomics. | shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. we sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval sardinian settlement of geridu. we obtained 6.5-fold coverage of a brucella melitensis genome. sequence reads from this genome showed signatures typical of ancient or aged dna. despite the relatively low coverage, we were ab ... | 2014 | 25028426 |
| typing discrepancy between phenotypic and molecular characterization revealing an emerging biovar 9 variant of smooth phage-resistant b. abortus strain 8416 in china. | a newly isolated smooth colony morphology phage-resistant strain 8416 isolated from a 45-year-old cattle farm cleaner with clinical features of brucellosis in china was reported. the most unusual phenotype was its resistance to two brucella phages tbilisi and weybridge, but sensitive to berkeley 2, a pattern similar to that of brucella melitensis biovar 1. vitek 2 biochemical identification system found that both strain 8416 and b. melitensis strains shared positive ilatk, but negative in other ... | 2015 | 26696984 |
| phospholipase a1 modulates the cell envelope phospholipid content of brucella melitensis, contributing to polymyxin resistance and pathogenicity. | a subset of bacterial pathogens, including the zoonotic brucella species, are highly resistant against polymyxin antibiotics. bacterial polymyxin resistance has been attributed primarily to the modification of lipopolysaccharide; however, it is unknown what additional mechanisms mediate high-level resistance against this class of drugs. this work identified a role for the brucella melitensis gene bvea (bmeii0681), encoding a predicted esterase, in the resistance of b. melitensis to polymyxin b. ... | 2015 | 26282427 |
| in situ characterization of splenic brucella melitensis reservoir cells during the chronic phase of infection in susceptible mice. | brucella are facultative intracellular gram-negative coccobacilli that chronically infect humans as well as domestic and wild-type mammals, and cause brucellosis. alternatively activated macrophages (m2a) induced by il-4/il-13 via stat6 signaling pathways have been frequently described as a favorable niche for long-term persistence of intracellular pathogens. based on the observation that m2a-like macrophages are induced in the spleen during the chronic phase of b. abortus infection in mice and ... | 2015 | 26376185 |
| multiple-locus variable-number tandem-repeat analysis of brucella isolates from patients in xinjiang china. | this study is to characterize and identify the human brucella strains in xinjiang, china with multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) scheme. | 2015 | 26629067 |
| detection and discrimination of two brucella species by multiplex real-time pcr and high-resolution melt analysis curve from human blood and comparison of results using rflp. | rapid and accurate detection of brucella abortus and brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. this study reveals a new method in detection of clinical samples of brucellosis using real-time pcr and high-resolution melt (hrm) curve analysis. | 2015 | 26523223 |
| mass spectrometry data from proteomics-based screening of immunoreactive proteins of fully virulent brucella strains using sera from naturally infected animals. | here, we provide the dataset associated with our research article on comprehensive screening of brucella immunoreactive proteins using sera of naturally infected hosts published in biochemical and biophysical research communications wareth et al., 2015 [1]. whole-cell protein extracts were prepared from brucella abortus and brucella melitensis, separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and subsequently western blotting was carried out using sera from bo ... | 2015 | 26322324 |
| multiple-locus variable-number tandem-repeat analysis (mlva) genotyping of human brucella isolates in malaysia. | brucellosis is one of the most common zoonotic diseases worldwide. it can cause acute febrile illness in human and is a major health problem. studies in human brucellosis in malaysia is limited and so far no genotyping studies has been done on brucella isolates. the aim of the study was to determine the genetic diversity among brucella species isolated from human brucellosis, obtained over a 6-year period (2009-2014). | 2015 | 26033227 |
| brucella melitensis biovar 1 and brucella abortus s19 vaccine strain infections in milkers working at cattle farms in the khartoum area, sudan. | human brucellosis is a preventable zoonoses that may become persistent, causing, if left untreated, severe localized disease. occupational exposure to infected animals or animal products and consumption of fresh contaminated dairy are main risk factors. | 2015 | 25938483 |
| immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from brucella melitensis. | brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. immunity against brucella melitensis involves antigen-specific cd4(+) and cd8(+) t-cells activation and humoral immune responses. due to negative aspects of live attenuated vaccines, much attention has been focused on finding brucella-protective antigens to introduce them as potential subunit vaccine candidates. | 2015 | 26019797 |
| modeling, molecular dynamics, and docking assessment of transcription factor rho: a potential drug target in brucella melitensis 16m. | the zoonotic disease brucellosis, a chronic condition in humans affecting renal and cardiac systems and causing osteoarthritis, is caused by brucella, a genus of gram-negative, facultative, intracellular pathogens. the mode of transmission and the virulence of the pathogens are still enigmatic. transcription regulatory elements, such as rho proteins, play an important role in the termination of transcription and/or the selection of genes in brucella. adverse effects of the transcription inhibito ... | 2015 | 25848225 |
| identification of a novel small non-coding rna modulating the intracellular survival of brucella melitensis. | bacterial small non-coding rnas (srnas) are gene expression modulators respond to environmental changes, stressful conditions, and pathogenesis. in this study, by using a combined bioinformatic and experimental approach, eight novel srna genes were identified in intracellular pathogen brucella melitensis. bsr0602, one srna that was highly induced in stationary phase, was further examined and found to modulate the intracellular survival of b. melitensis. bsr0602 was present at very high levels in ... | 2015 | 25852653 |
| full genome snp-based phylogenetic analysis reveals the origin and global spread of brucella melitensis. | brucellosis is an important zoonotic disease that affects both humans and animals. we sequenced the full genome and characterised the genetic diversity of two brucella melitensis isolates from malaysia and the philippines. in addition, we performed a comparative whole-genome single nucleotide polymorphism (snp) analysis of b. melitensis strains collected from around the world, to investigate the potential origin and the history of the global spread of b. melitensis. | 2015 | 25888205 |
| decreased in vivo virulence and altered gene expression by a brucella melitensis light-sensing histidine kinase mutant. | brucella species utilize diverse virulence factors. previously, brucella abortus light-sensing histidine kinase was identified as important for cellular infection. here, we demonstrate that a brucella melitensis lov-hk (bm-lov-hk) mutant strain has strikingly different gene expression than wild type. general stress response genes including the alternative sigma factor rpoe1 and its anti-anti-sigma factor phyr were downregulated, while flagellar, quorum sensing (qs), and type iv secretion system ... | 2015 | 25132657 |
| improved serodiagnosis of bovine brucellosis by novel synthetic oligosaccharide antigens representing the capping m epitope elements of brucella o-polysaccharide. | members of the genus brucella have cell wall characteristics of gram-negative bacteria, which in the most significant species includes o-polysaccharide (ops). serology is the most cost-effective means of detecting brucellosis, as infection with smooth strains of brucella leads to the induction of high antibody titers against the ops, an unbranched homopolymer of 4,6-dideoxy-4-formamido-d-mannopyranosyl residues (d-rha4nfo) that are variably α(1→2)- and α(1→3)-linked. six d-rha4nfo homo-oligosacc ... | 2015 | 25653412 |
| a new method for simultaneous gene deletion and down-regulation in brucella melitensis rev.1. | in this study, our aim was to integrate an antisense expression cassette in bacterial chromosome for providing a long-term expression down-regulation in a bid to develop a new approach for simultaneous deletion and down-regulation of target genes in bacterial system. therefore, we were used this approach for simultaneous deletion of the perosamine synthetase (per) gene and down-regulation of the virb1 expression in brucella melitensis rev.1. the per gene, which is one of the lps o-chain coding g ... | 2015 | 25249309 |
| emergence of quinolone-resistant, topoisomerase-mutant brucella after treatment with fluoroquinolones in a macrophage experimental infection model. | to determine the activity of fluoroquinolones (fq) and the selection of fq-resistant mutants in a macrophage experimental infection model (meim). | 2015 | 24913992 |
| hospital-associated transmission of brucella melitensis outside the laboratory. | brucella melitensis was identified in an aspirate obtained from a patient's hip joint during a procedure at a hospital in canada. we conducted an investigation into possible exposures among hospital workers; 1 worker who assisted with the procedure tested positive for b. melitensis. aerosol-generating procedures performed outside the laboratory may facilitate transmission of this bacterium. | 2015 | 25531198 |
| financial analysis of brucellosis control for small-scale goat farming in the bajío region, mexico. | brucellosis is an endemic disease in small-scale goat husbandry systems in mexico. it is a zoonosis and the economic consequences can be large, although estimates are not available for the mexican goat sector. our objective was to conduct a financial analysis of brucellosis control in a prominent dairy goat production area of the bajío region, mexico. we used three models: (1) a brucellosis transmission model at village flock level (n=1000 head), (2) a flock growth model at smallholder flock lev ... | 2015 | 25529385 |
| molecular cloning, expression and characterization of programmed cell death 10 from sheep (ovis aries). | programmed cell death 10 (pdcd10) is a highly conserved adaptor protein. its mutations result in cerebral cavernous malformations (ccms). in this study, pdcd10 cdna from the buffy coat of small tail han sheep (ovis aries) was cloned from a suppression subtractive hybridization cdna library, named oapdcd10. the full-length cdna of oapdcd10 was 1343bp with a 639bp open reading frame (orf) encoding 212 amino acid residues. tissue distribution of oapdcd10 mrna determined that it was ubiquitously exp ... | 2015 | 25541025 |
| brucella infection of the thoracic vertebral arch presenting with an epidural abscess: a case report. | although brucella spondylitis and brucella discitis have been frequently reported, brucella infection of the vertebral arch is rare and has not been previously described. we present the first case of brucella infection of the thoracic vertebral arch with epidural abscess formation and discuss the clinical key points. | 2015 | 26496896 |
| novel vaccine candidates against brucella melitensis identified through reverse vaccinology approach. | global health therapeutics is a rapidly emerging facet of postgenomics medicine. in this connection, brucella melitensis is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. presently, no licensed vaccines are available for human brucellosis. here, we report the identification of potential vaccine candidates against b. melitensis using a reverse vaccinology approach. based on a systematic screening of exoproteome and secretome of b. melitensis 16 m, we identifi ... | 2015 | 26479901 |
| a brucella melitensis m5-90 wboa deletion strain is attenuated and enhances vaccine efficacy. | brucella spp. are gram-negative intracellular pathogens of both humans and animals that cause great economic burdens in developing countries. live attenuated vaccines are the most efficient means for the prevention and control of animal brucellosis. however, brucella vaccines (strain m5-90 and others) have several drawbacks and do not allow serological differentiation between vaccinated and infected animals. a wboa mutant was derived from brucella melitensis (b. melitensis) vaccine strain m5-90 ... | 2015 | 25899866 |
| immunogenic response to a recombinant pseudorabies virus carrying bp26 gene of brucella melitensis in mice. | brucellae are facultative intracellular bacterial pathogens of a zoonotic disease called brucellosis. live attenuated vaccines are utilized for prophylaxis of brucellosis; however, they retain residual virulence to human and/or animals, as well as interfere with diagnosis. in this study, recombinant virus prv δtk/δge/bp26 was screened and purified. one-step growth curve assay showed that the titer of recombinant virus was comparable to the parent strain. mice experiments showed the recombinant v ... | 2015 | 25890577 |
| brucellosis in nomadic pastoralists and their goats in two provinces of the eastern algerian high plateaus. | a 31-months study was conducted to elucidate the prevalence of brucellosis in nomadic pastoralists and their goats in two provinces of the eastern algerian high plateaus. five hundred eight human and 4955 animal sera were screened with the rose bengal plate test and the complement fixation test for confirmation. uterine fluids from aborting goats were subjected to microbiological analyses to determine the biovars responsible for abortions. the overall seroprevalence was 0.98% among animals and 1 ... | 2015 | 25877260 |
| in vitro evaluation of six chemical agents on smooth brucella melitensis strain. | brucellosis is a zoonosis that disseminated by a variety of ways between animals and humans. the effective disinfection of contaminated environments, soil, feces, and animal bodies plays an irreplaceable role in the prevention and control of brucellosis. to kill brucella effectively, the bactericidal effects of frequently used disinfectants (including aldehydes, halogens, quaternary ammonium compound, phenolics, and alkalines) and the potential factors that influence disinfection effects were de ... | 2015 | 25857255 |
| complete genome-wide screening and subtractive genomic approach revealed new virulence factors, potential drug targets against bio-war pathogen brucella melitensis 16m. | brucella melitensis 16m is a gram-negative coccobacillus that infects both animals and humans. it causes a disease known as brucellosis, which is characterized by acute febrile illness in humans and causes abortions in livestock. to prevent and control brucellosis, identification of putative drug targets is crucial. the present study aimed to identify drug targets in b. melitensis 16m by using a subtractive genomic approach. we used available database repositories (database of essential genes, k ... | 2015 | 25834405 |
| epidemiological, diagnostic, clinical, and therapeutic aspects of brucella bacteremia in children in southern israel: a 7-year retrospective study (2005-2011). | data on the various aspects of brucellosis in children living in southern israel are missing. | 2015 | 25793475 |
| the prevalence and distribution of brucella melitensis in goats in malaysia from 2000 to 2009. | a study was conducted to describe the prevalence and distribution of zoonotic brucella melitensis in goats in peninsular malaysia. using serosurveillance data of the last decade (2000-2009) involving 119,799 goats and 3555 farms, the seroprevalence of brucellosis among goats was 0.91% (95% ci=0.86-0.96) and among farms was 7.09% (95% ci=6.27-7.98). the odds of brucellosis was significantly (p<0.05) higher in the later part of the decade, in larger herd size and among the states located in the pe ... | 2015 | 25746928 |
| tcesr two-component regulatory system of brucella melitensis 16m is involved in invasion, intracellular survival and regulated cytotoxicity for macrophages. | the mechanisms of invasion and intracellular survival of brucella are still poorly understood. previous studies showed that the two-component regulatory systems (tcss) play an important role in the intracellular survival of brucella. to investigate if tcss involve in the virulence and cytotoxicity of brucella melitensis, we introduced a mutation into one of the tcss in chromosome ii in br. melitensis 16m strain, and generated 16mδtcesr, a mutant of br. melitensis 16m strain. in vitro infection e ... | 2015 | 25721466 |
| full-length cdna cloning, molecular characterization and differential expression analysis of peroxiredoxin 6 from ovis aries. | peroxiredoxin 6 (prdx6), an important antioxidant enzyme that can eliminate reactive oxygen species (ros) to maintain homeostasis, is a bifunctional protein that possesses the activities of both glutathione peroxidase and phospholipase a2. in this study, a novel full-length prdx6 cdna (oaprdx6) was cloned from sheep (ovis aries) using rapid amplification of cdna ends (race). the full-length cdna of oaprdx6 was 1753bp containing a 5'-untranslated region (utr) of 93bp, a 3'-utr of 985bp with a pol ... | 2015 | 25712755 |
| in vitro activity of brucella melitensis isolates to various antimicrobials in turkey. | brucellosis is a systemic infectious disease caused by brucella bacteria. a successful treatment requires antibiotics that can penetrate into the cell at high concentrations. the aim of this study was to assess the biotype and in vitro activity of 80 brucella isolates obtained from blood against various antimicrobials for human brucellosis in turkey. | 2015 | 25712728 |
| acute brucella melitensis m16 infection model in mice treated with tumor necrosis factor-alpha inhibitors. | there is limited data in the literature about brucellosis related to an intracellular pathogen and anti-tumor necrosis factor alpha (anti-tnfα) medication. the aim of this study was to evaluate acute brucella infections in mice receiving anti-tnfα drug treatment. | 2015 | 25699488 |
| using molecular tools to identify the geographical origin of a case of human brucellosis. | although malta is historically linked with the zoonosis brucellosis, there had not been a case of the disease in either the human or livestock population for several years. however, in july 2013 a case of human brucellosis was identified on the island. to determine whether this recent case originated in malta, four isolates from this case were subjected to molecular analysis. molecular profiles generated using multilocus sequence analysis and multilocus variable number tandem repeat for the rece ... | 2015 | 25697304 |
| the effects of regm on stress responses in brucella melitensis. | brucella melitensis is a facultative intracellular pathogen. the regm gene encodes a sensory transduction protein kinase in b. melitensis 16m, and genes orthologous to regm have been found to exist in many bacterial species. however, little is known about the regulation function of this gene in brucella. in order to characterize this gene, we constructed a marked deletion mutant of regm as well as its complemented strain. the mutant was less able to withstand acid and hyperosmotic conditions tha ... | 2015 | 25648428 |
| genotyping of human brucella melitensis biovar 3 isolated from shanxi province in china by mlva16 and hoof. | brucellosis presents a significant economic burden for china because it causes reproductive failure in host species and chronic health problems in humans. these problems can involve multiple organs. brucellosis is highly endemic in shanxi province china. molecular typing would be very useful to epidemiological surveillance. the purpose of this study was to assess the diversity of brucella melitensis strains for epidemiological surveillance. historical monitoring data suggest that brucella melite ... | 2015 | 25615697 |
| rna-seq reveals the critical role of otpr in regulating brucella melitensis metabolism and virulence under acidic stress. | the response regulator otpr is critical for the growth, morphology and virulence of brucella melitensis. compared to its wild type strain 16 m, b. melitensis 16 mδotpr mutant has decreased tolerance to acid stress. to analyze the genes regulated by otpr under acid stress, we performed rna-seq whole transcriptome analysis of 16 mδotpr and 16 m. in total, 501 differentially expressed genes were identified, including 390 down-regulated and 111 up-regulated genes. among these genes, 209 were associa ... | 2015 | 26242322 |
| vaccination schedules in small ruminant farms. | development and implementation of health management plans is the cornerstone of profitable farms; prevention of microbial diseases by means of vaccination is an integral part of such a plan. in every production type and management system in small ruminants, microbial diseases have a major significance, hence their proper control must be based in good health management practices, including use of effective and safe vaccines. development of various types of vaccines is evolving very quickly in rec ... | 2015 | 26220514 |
| mlva as an epidemiological tool to trace back brucella melitensis biovar 1 re-emergence in italy. | brucellosis is an important zoonosis caused by brucella spp., still prevalent in most areas of the world. brucellosis control in animals is the key to protect humans. the knowledge of brucella spp. prevailing genotypes in a territory represents an important epidemiological tool to formulate policies and strategies for disease control and to trace back the introduction of new strains previously considered as exotic. in the last years, multiple-locus variable number tandem repeat analysis (mlva) h ... | 2015 | 26194658 |
| a familial cluster of human brucellosis attributable to contact with imported infected goats in shuyang, jiangsu province, china, 2013. | brucellosis remains a serious public health issue in developing countries, including china. on august 8, 2013, four cases of brucellosis from one extended family were reported at shuyang county, jiangsu province, china. active case finding was performed to identify the source and the risk factors of the infection and to prevent additional cases. multiple-locus variable number tandem repeat analysis (mlva) was used for molecular subtyping analysis. six people from two extended families met the ca ... | 2015 | 26149866 |
| brucella infection in a child with progressive familial intrahepatic cholestasis type 2 who had undergone liver transplantation. | brucellosis is considered the most widespread zoonosis in the world. in endemic regions of brucellosis, childhood brucellosis includes up to one-third of all cases of human brucellosis. brucellosis constitutes a public health problem in turkey. a boy aged 12 yr who had pfic2 had undergone deceased-donor liver transplantation in 2008 at the age of seven. the boy presented with fatigue, fever, and pain in the right leg and hip and was admitted to the hospital. brucella melitensis grew in the blood ... | 2015 | 26148181 |
| cloning, molecular analysis and epitopics prediction of a new chaperone groel brucella melitensis antigen. | brucellosis is a well-known domestic animal infectious disease, which is caused by brucella bacterium. groel antigen increases brucella survival and is one of the major antigens that stimulates the immune system. hence, the objective of the present study was cloning and bioinformatics analysis of groel gene. | 2015 | 26124937 |
| a case of brucellosis presenting with acute hepatitis and bicytopenia. | although liver involvement is frequently seen in brucellosis, acute hepatitis is a rare clinical entity. in its progress, haematological findings are non-specific and vary in respect to severity. in this paper, we present a case of brucellosis with acute hepatitis and bicytopenia without anaemia. a 19-year-old man presented with a 2-week history of fever, sweating, low back and leg pain, lassitude, loss appetite, nausea and vomiting. he gave a history of raw milk ingestion and animal contact. ph ... | 2015 | 26110300 |
| in vitro antimicrobial susceptibility testing of human brucella melitensis isolates from qatar between 2014 - 2015. | brucellosis is one of the most common zoonotic disease affecting humans and animals and is endemic in many parts of the world including the gulf cooperation council region (gcc). the aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of brucella strains isolated from clinical specimens, from qatar. | 2015 | 26073177 |
| isolation of brucella melitensis from a human case of chronic additive polyarthritis. | brucellar arthritis remains under diagnosed owing to non-specific clinical manifestations. here, we report isolation of brucella melitensis from synovial fluid of 5th metatarsophalangeal joint of a 39-year-old lady having unusually chronic asymmetric, additive, peripheral polyarthritis. this isolation was confirmed by bruce-ladder polymerase chain reaction (pcr). the patient had a history of contact with an aborted goat. rose bengal plate agglutination test (rbpt) and standard tube agglutination ... | 2015 | 26068351 |
| immune responses and protection induced by brucella suis s2 bacterial ghosts in mice. | with the purpose of generating brucella suis bacterial ghosts and investigating the immunogenicity of bacterial ghosts as a vaccine candidate, the lysis gene e and temperature-sensitive regulator cassette were cloned into a shuttle plasmid, pbbr1mcs-2, for construction of a recombinant temperature-sensitive shuttle lysis plasmid, pbbr1mcs-e. pbbr1mcs-e was then introduced into attenuated b. suis live vaccine s2 bacteria, and the resultant transformants were used for production of b. suis ghosts ... | 2015 | 26022514 |
| a multicopper oxidase contributes to the copper tolerance of brucella melitensis 16m. | copper is a potent antimicrobial agent. multiple mechanisms of copper tolerance are utilized by some pathogenic bacteria. bmeii0580, which is significantly similar to the multicopper oxidase from escherichia coli, was predicted to be the probable blue copper protein yack precursor in brucella melitensis 16m, and was designated as brucella multicopper oxidase (bmco). a bioinformatics analysis indicated that the typical motifs of multicopper oxidases are present in bmco. bmco, the expression of wh ... | 2015 | 25956175 |
| pathologic basis of pyogenic, nonpyogenic, and other spondylitis and discitis. | pyogenic spondylitis and discitis are usually seen following a recent infection or surgery. a septic embolus causes an infarcted area within the bone. pyogenic spondylitis is characterized by edema, vascular leakage, and supportive inflammatory reaction characterized with polymorphonuclear leukocytes. in tuberculosis of the spine, active lesions are characterized by formation of epithelioid granulomas with central caseating necrosis. mycobacterium tuberculosis can be shown by histochemical stain ... | 2015 | 25952170 |
| a retrospective study of 39 patients treated with anterior approach of thoracic and lumbar spondylodiscitis: clinical manifestations, anterior surgical treatment, and outcome. | the aim of this study is to report our 39 patients treated with anterior debridement and autologous iliac bone grafting with or without anterior instrumentation, which is the presumed treatment of choice for thoracic or lumbar spondylodiscitis.our patients underwent surgical treatment of spondylodiscitis using anterior debridement and autologous iliac bone grafting with or without anterior instrumentation and were analyzed with a mean follow-up of 8 years (range, 2-11 years). kaneda 2-rod system ... | 2015 | 26632729 |
| [undulant fever and autoimmune hemolytic anemia in a 20-year-old exchange student from jordan - the human brucellosis as an important differential diagnosis in migrants]. | a 20-year-old jordanian exchange student presents with recurrent fever, night sweats, cough, and swelling and redness around the ankle. physical examination further reveals bilateral ankle arthritis and painful cervical lymphadenopathy. | 2015 | 26625239 |
| brucella melitensis 16mδtcfsr as a potential live vaccine allows for the differentiation between natural and vaccinated infection. | brucellosis is a zoonotic disease that poses a serious threat to public health and safety. although the live attenuated vaccines targeting brucellosis, such as m5-90, are effective, there are a number of drawbacks to their use. for example, the vaccines are unable to differentiate between the natural and vaccinated forms of the infection, and these vaccines have also been shown to cause abortion in pregnant animals. therefore, a safer and more potent vaccine is required. in the present study, a ... | 2015 | 26622461 |