Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the clinical picture presented by premature neonates infected with the respiratory syncytial virus. | during the course of a 3-year epidemic of respiratory syncytial virus (rsv) in an neonatal intensive care unit, we examined premature neonates for the clinical features of rsv infections and the risk factors predisposing towards transmission of the disease. seventy-nine infants with a median gestational age of 31 weeks were tested in 113 episodes of clinical deterioration for the presence of rsv antigen in nasopharyngeal secretions. forty-seven results were positive and 66 negative. bradycardia, ... | 1995 | 8582403 |
| evidence that the amino acid region 124-203 of glycoprotein g from the respiratory syncytial virus (rsv) constitutes a major part of the polypeptide domain that is involved in the protection against rsv infection. | the first 230 residues of the 298-amino acid glycoprotein g of respiratory syncytial virus (rsv) are sufficient to confer complete resistance to challenge with live rsv, whereas the first 180 residues completely failed (olmsted et al. (1989) j. virol. 63, 411-420). the characterization of a protective epitope corresponding to the amino acid region 174-187 of the g protein (trudel et al. (1991) virology 185, 749-757) suggests that interruption of this region in the 180 residue truncated polypepti ... | 1995 | 8669890 |
| effect of orthovanadate on commitment of avian myoblasts transformed with rous sarcoma virus to myogenic differentiation. | myogenic differentiation of quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells) depends on the temperature: at 35.5 degrees c, the permissive temperature for the virus, the transformed myoblasts proliferate, without fusion, but at 41 degrees c, the nonpermissive temperature, they become committed to myogenic differentiation until about 10 h and then myoblast fusion occurs within 24 h. this temperature dependency of the differentiation reaction is ... | 1995 | 8603684 |
| isolation of virus-neutralizing rnas from a large pool of random sequences. | rna and ribonuclease-resistant rna analogs that bound and neutralized rous sarcoma virus (rsv) were isolated from a large pool of random sequences by multiple cycles of in vitro selection using infectious viral particles. the selected rna pool of rsv-binding sequences at a concentration of 0.16 microm completely neutralized the virus. of 19 sequences cloned from the selected pool, 5 inhibited rsv infection. the selected rna and rna analogs were shown to neutralize rsv by interacting with the vir ... | 1995 | 8524793 |
| production of infectious human respiratory syncytial virus from cloned cdna confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the m2 mrna in gene expression and provides a capability for vaccine development. | infectious human respiratory syncytial virus (rsv) was produced by the intracellular coexpression of five plasmid-borne cdnas. one cdna encoded a complete positive-sense version of the rsv genome (corresponding to the replicative intermediate rna or antigenome), and each of the other four encoded a separate rsv protein, namely, the major nucleocapsid n protein, the nucleocapsid p phosphoprotein, the major polymerase l protein, or the protein from the 5' proximal open reading frame of the m2 mrna ... | 1995 | 8524804 |
| a plasmid-based self-amplifying sindbis virus vector. | sindbis virus was used as a self-amplifying eukaryotic expression vector. a recombinant cdna genome of this (+)-strand rna virus was placed under the transcriptional control of a rous sarcoma virus ltr (rsv) promoter. transfection of this plasmid construct into mammalian cell lines (3t3, hepg2, and 293 cells) resulted in expression of the luciferase reporter gene. high-expression levels were also measured after transfection into primary rat myoblasts. in differentiated myotubes, expression level ... | 1995 | 8527474 |
| combination therapy with aerosolized ribavirin and intravenous immunoglobulin for respiratory syncytial virus disease in adult bone marrow transplant recipients. | respiratory syncytial virus (rsv) infections in adult bmt recipients are frequently complicated by fatal pneumonias. therapy of rsv pneumonia with aerosolized ribavirin alone has been reported to be associated with a 70% mortality rate. because immune globulin therapy has been reported to be beneficial, we conducted a prospective trial of combination therapy with aerosolized ribavirin and intravenous immunoglobulin (ivig). aerosolized ribavirin was administered at 20 mg/ml for 18 h a day and ivi ... | 1995 | 8535312 |
| inhibitory effect of pyridobenzazoles on virus replication in vitro. | twelve species of pyridobenzazoles and pyrimidobenzimidazole were examined as inhibitors of the replication of respiratory syncytial virus (rsv) in hela cells. from the pyridobenzazoles studied, 2-benzamido-4-cyano-1-oxo-1h,5h-pyrido[1,2-alpha]benzimidazol emerged as a potent inhibitor of the rsv. based on its inhibitory effect on the cytopathogenicity of the rsv in hela cells, the 50% effective dose was found to be 0.95 micrograms/ml. the cytotoxicity of this compound for hela cells was examine ... | 1995 | 8535400 |
| orthomyxoviral and paramyxoviral infections in transplant patients. | in summary, orthomyxo- and paramyxoviruses cause clinically important infections in transplant patients. patients often develop lower respiratory tract involvement and sometimes respiratory failure, which almost is uniformly fatal. bone marrow transplant recipients appear to be at higher risk of severe disease than are solid-organ recipients, but well defined criteria to predict those patients who will be severely affected are not available. factors associated with more severe disease include th ... | 1995 | 8747776 |
| approaching the rsv season with a nursing plan of action. | as the statistics show with year-in, year-out regularity, during november through march in the united states, approximately 90,000 infants and young children will be hospitalized with a severe lower respiratory infection attributable to the respiratory syncytial virus (rsv). this virus, discovered only as recently as 1956, appears to be ubiquitous, infecting virtually 100% of children by age 4. for most of them the resulting illness will be mild and easily vanquished by an intact immune system. ... | 1995 | 8700601 |
| adenovirus-mediated expression of a reporter gene in thalamocortical cocultures. | organotypic cocultures of thalamic and cortical explants have recently been used to study the development of the thalamocortical axonal network in the mammalian neocortex. to explore the possibility of genetically manipulating organotypic explants, rat thalamocortical (tc) cocultures were infected with the recombinant adenovirus, adv/rsv beta gal. infection of the cortical explants resulted in long-term expression (2 weeks) of the reporter gene (beta-galactosidase) with no significant alteration ... | 1995 | 8719624 |
| activation of complement in quails bearing rous sarcoma virus-induced tumors. | the concentration of serum c3 in quails bearing tumors induced by rous sarcoma virus (rsv) was elevated in parallel with tumor growth, whereas serum c3 levels in quails inoculated with avian leukosis virus, which lacks transforming activity, showed a pattern similar to that in mock-infected quails. c3 deposition was also observed in almost all tumor cells at the tumor developing stage. these findings obtained in vivo suggest that the cells transformed by rsv activated the c3 on their surfaces. | 1995 | 8720051 |
| respiratory syncytial virus and parainfluenza virus infections in the immunocompromised host. | respiratory syncytial virus (rsv) and parainfluenza virus (piv) are common causes of respiratory infections in immunocompetent children under the age of 6 years. these viruses belong to a family of enveloped single-stranded rna viruses, the paramyxoviruses. the clinical manifestations in the normal host range from mild illness to severe croup, bronchiolitis, and pneumonia. after the age of 6 years, reinfections occur, but are characterized by diminishing frequency and severity. in contrast to th ... | 1995 | 8668850 |
| production of defective virus by terminally differentiated myotubes infected with rous sarcoma virus. | the generally accepted concept that the replication of rous sarcoma virus (rsv) is dependent on host cell dna synthesis was reexamined. as the host we used terminally differentiated myotubes (mt), in which no cellular dna synthesis is observed. as an extension of our previous study which indicated that rsv-infected mt produce various virus components, we examined viral particles produced by infected mt. electron microscopy showed presence of viral particles released from infected mt. immunopreci ... | 1995 | 8825300 |
| expression of the herpes simplex virus type 1 glycoprotein e in human cells and in escherichia coli: protection studies against lethal viral infection in mice. | the objective of this study was to examine the protective efficacy of purified recombinant herpes simplex virus type 1 (hsv-1) glycoprotein e (ge-1) in the mouse lethal challenge model. a secreted form of ge-1 (hge-1s) protein, containing amino acids 1-406, was produced in human cells by using the episomal replicating prp-rsv expression vector. in addition, a portion of the ge-1 (bge-1t) protein corresponding to amino acids 90-406, was expressed in escherichia coli as a fusion protein with malto ... | 1995 | 8847521 |
| vaccination with a low-oncogenic strain of rous sarcoma virus prevents visceral tumors in chickens. | the oncogenic potential of different strains of rous sarcoma virus (rsv) varies significantly in two mhc(b) congenic chicken lines cb and cc and in their f1 hybrids with the unrelated inbred line ia. the bryan high-titer pseudotype of rsv of antigenic subgroups d and b (bh-d, and bh-b) was highly oncogenic, eliciting mostly fatal, progressively growing tumors. visceral tumor formation and erythroblastosis were seen after challenge with bh-b in cb and cc chickens, but not in their f1 hybrids with ... | 1995 | 8919361 |
| efficient expression of pro-urokinase by human lymphoblastoid namalwa kjm-1 cells using moloney retroviral promoter. | we have compared the level of expression of several enhancer/promoters in human lymphoblastoid namalwa kjm-1 cells when fused to a common reporter gene. a cassette containing the pro-urokinase (pro-uk) coding sequence followed by the rabbit beta-globin and simian virus 40 (sv40) 3' nontranslated region was used for evaluation of the enhancer activity. cells containing moloney murine leukemia virus (mo-mulv) promoter had an average of 10-20 fold higher expression levels of pro-uk than those conta ... | 1995 | 8920107 |
| a colorimetric ldh assay for the titration of infectivity and the evaluation of anti-viral activity against ortho- and paramyxoviruses. | a rapid and precise screening assay was developed for in vitro evaluation of anti-orthomyxo- and anti-paramyxovirus agents. the procedure is spectrophotometrical assessment for viability of cells via extracellular leakage of lactic dehydrogenase (ldh). hmv-ii cells, a human melanoma cell line was found to be suitable for the titration of virus infectivity and screening of anti-viral agents for orthomyxo- and paramyxoviruses. comparative titration of infectivity of stock viruses by the ldh and th ... | 1995 | 8928191 |
| evaluation of subgroup-specific peptides of the g protein of respiratory syncytial virus for characterization of the immune response. | two synthetic peptides, designated peptides 12g(a) and 12g(b), representing amino acids 174-188 of the g glycoprotein of respiratory syncytial virus (rsv) subgroup a (strain a2) and subgroup b (strain ch18537) were evaluated for their properties as subgroup-specific antigens for enzyme immunoassay (elisa). these peptides were used to characterize the immune response of children with naturally occurring rsv infection during six annual epidemics in the huntington area, west virginia, usa; viz. 197 ... | 1995 | 8830114 |
| synthesis and antiviral activities of 9-cyclopentylpurine derivatives. | the synthesis and anti-rsv (rouse sarcoma virus) activity of hmca, (+/-)-9-(2-hydroxymethyl-cyclopenthyl)-adenine, and its derivatives are described. it has been demonstrated that trans-hmca has greater anti-rsv activity in tissue culture than cis-hmca. | 1995 | 8841575 |
| respiratory syncytial virus infection: clinical presentation and management. | rsv infection continues to be a major cause of morbidity and mortality throughout the world. despite advances in the understanding of its pathogenesis, limited progress has been made in prevention and treatment of rsv infection. based on the experiences thus far it seems that control of rsv infection will be a difficult and complex task. | 1995 | 10829841 |
| application of elisa for igm, iga and antigen detection for rapid diagnosis of respiratory syncytial virus infections: a comparative study. | infection with respiratory syncytial virus (rsv) can be rapidly diagnosed by detection of viral antigen in nasopharyngeal secretions (nps) or serologically by detecting igm and iga antibodies. | 1995 | 15566784 |
| antimicrobial activity of lipids added to human milk, infant formula, and bovine milk. | lipids previously shown to have antiviral and antibacterial activity in buffers were added to human milk, bovine milk, and infant formulas to determine whether increased protection from infection could be provided to infants as part of their diet. fatty acids and monoglycerides with chain lengths varying from 8 to 12 carbons were found to be more strongly antiviral and antibacterial when added to milk and formula than long chain monoglycerides. lipids added to milk and formula inactivated a numb ... | 1995 | 12049996 |
| suppression and restoration of v-src expression in rsv transformed-cells after transfection with n-ras and its antagonist. | previously, it was shown that hamster cells transformed by rous sarcoma virus (rsv) exhibited a decreased expression of the rsv products (including the pp60 src oncogene) when these cells were supertransfected with the n-ras oncogene. to assess the responsibility of the activated n-ras in the modulation of the rsv viral products, a strategy based on two ras antagonists was used; i.e. i) a rap1a/k-rev1 expression vector known for its capacity to revert the k-ras induced transformed phenotype and ... | 1995 | 21552859 |
| safety aspects of vitamin a administration. | to review longstanding experience in the safe use of vitamin a as therapeutic agent in prematurely born human neonates and more recently with young children infected with the respiratory syncytial virus (rsv). | 1996 | 8841769 |
| characteristics of il-6 and tnf-alpha production by respiratory syncytial virus-infected macrophages in the neonate. | the production of il-6 and tnf-alpha and the expression of their mrna were studied with neonatal (cord blood) and adult blood monocyte-derived macrophages (mdm) after in vitro infection with respiratory syncytial virus (rsv). cord blood mdm exhibited production of high levels of il-6 within 24 hr after infection. little or no il-6 production was detected after 24-48 hr and after in vitro stimulation with inactivated (nonreplicating) virus. adult blood mdm also produced high levels of il-6 within ... | 1996 | 8835355 |
| stabilization of respiratory syncytial virus (rsv) against thermal inactivation and freeze-thaw cycles for development and control of rsv vaccines and immune globulin. | a high-titered and stable respiratory syncytial virus (rsv) is essential for the development of rsv vaccines and quality control of vaccines and rsv immune globulin. we increased the virus titer of rsv seed stock, and virus preparations made from this seed stock, 100 times by removing defective interfering particles using limiting dilution procedure. rsv preparations made from the new seed stock had infectivity titers ranging from 10(7.6) to 10(8.2) tcid50 per ml for five lots made over a period ... | 1996 | 8994316 |
| molecular characterization of the complex sex-chromosome heterochromatin in the rodent microtus chrotorrhinus. | the sex chromosomes of microtus chrotorrhinus are unusually large compared to those of other mammals, comprising about 20% of the karyotype and containing substantial amounts of constitutive heterochromatin. previous studies have described two highly repeated dna families (msat-160 and msat-2570) that localize to this heterochromatin (modi, 1992, 1993c). the present report describes a third satellite dna family (termed msat-21) in m. chrotorrhinus that is also located in the sex heterochromatin. ... | 1996 | 8995489 |
| inducible retroviral vectors regulated by lac repressor in mammalian cells. | we have developed lac repressor-regulated retroviral expression vectors that are induced by beta-galactosides upon stable transduction in mammalian cells. these vectors, derived from a moloney-virus-based vector lncx, contain an internal rous sarcoma virus (rsv) or cytomegalovirus (cmv) promoter, coupled with 2 to 4 lac operator sequences and placed in anti orientation relative to the retroviral long terminal repeat (ltr). three different vectors were tested in stably infected mass populations o ... | 1996 | 8996098 |
| mechanism of rsv-induced il-8 gene expression in a549 cells before viral replication. | previous studies demonstrated that respiratory syncytial virus (rsv) infection of a549 cells induced interleukin (il)-8 gene expression and protein release from the cells as early as 2 h after treatment [m. a. fiedler, k. wernke-dollries, and j. m. stark. am. j. physiol. 269 (lung cell. mol. physiol. 13): l865-l872, 1995; j. g. mastronarde, m. m. monick, and g. w. hunninghake. am. j. respir. cell mol. biol. 13: 237-244, 1995]. furthermore, the effects of rsv at the 2-h time point were not depend ... | 1996 | 8997267 |
| identification of potential ctl epitopes of bovine rsv using allele-specific peptide motifs from bovine mhc class i molecules. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young infants and housed calves. depletion of cd8+ lymphocytes from calves inhibited their ability to clear the virus from the nasopharynx and lungs. to study these cells further, a cytotoxic t lymphocyte (ctl) assay was established. ctl could be demonstrated in the peripheral blood of gnotobiotic calves 7-10 days post infection (p.i.) with rsv and in lungs 10 days p.i. this response was both mhc-restricte ... | 1996 | 8988867 |
| isolation of viruses from clinical specimens in microtitre plates with cells inoculated in suspension. | virus isolation is essential for the provision of a full diagnostic virology service. present methods are time consuming, expensive and relatively inflexible for routine use. our objective was to audit our existing virus isolation system and to develop a sensitive, flexible virus isolation system which could be adapted for use in a busy routine laboratory which is required to provide a service for a wide range of clinical situations. we carried out a pilot study which compared conventional rolle ... | 1996 | 9002075 |
| white blood cell count, c-reactive protein and erythrocyte sedimentation rate in respiratory syncytial virus infection of the lower respiratory tract. | laboratory findings such as white blood cell (wbc) count, c-reactive protein (crp) concentration and erythrocyte sedimentation rate (esr) level in patients with bronchiolitis, bronchopneumonia and lobar pneumonia caused by respiratory syncytial virus (rsv) were studied. the diagnosis of having rsv infection of the lower respiratory tract was made on the presence of rsv antigen in nasopharyngeal specimens by means of enzyme immunoassay, on chest x-ray appearances and clinical manifestations. the ... | 1996 | 9002292 |
| prevalence of antibodies against hep-2 cell antigen in infants and children hospitalized with respiratory syncytial virus infection. | infants with respiratory syncytial virus (rsv) infection were shown to have antibodies against hep-2 cell antigen present in rsv-antigen preparation used for immunoblot analysis. the prevalence of anti hep-2 cell antibodies was examined in infants hospitalized for rsv infection (n = 49, median age 121 days) compared to rotavirus infected children (n = 30, median age 114 days) and to healthy controls (n = 20, median age 150 days). the immunoblot analysis with rsv-infected and non-infected hep-2 c ... | 1996 | 9007586 |
| two tumor-associated membrane antigens defined by monoclonal antibodies in a transplantable sarcoma induced by rous sarcoma virus in rat. | two hybridoma clones have been produced by hybridization of murine myeloma cell line pai and splenocytes from balb/c mice immunized with cells from a transplantable sarcoma induced in rat by sr-rsv. the antibody produced by hybridoma clone 2c2 was of subclass igg3 and recognized a cell surface antigen of 52 kd. it only cross-reacted with cells from sr-rsv-induced sarcoma in hamster, but not with cells from the chicken rsv-induced sarcoma, nor with a number of methylcholanthrene sarcomas and vari ... | 1996 | 8931754 |
| preventing nosocomial infection in paediatric wards. | nosocomial infection is potentially a major problem in paediatric wards. however, there are methodological difficulties which hinder researchers in identifying the true extent of nosocomial infection. respiratory infections pose the greatest challenge, with the respiratory syncytial virus (rsv) being particularly problematic. however, rsv can be contained by strict implementation of isolation procedures. other major pathogens such as enteric bacteria and viruses can similarly be controlled throu ... | 1996 | 8932032 |
| laboratory diagnosis of acute lower respiratory tract viral infections in children. | this report summarizes and compares the results of complement fixation test (cft), virus isolation (vi), and direct immunofluorescence test (dif) for the antigen detection of respiratory syncytial virus (rsv), influenza a virus (flu a), and adenovirus in 62 children with acute lower respiratory tract infections (alri) in kuwait. it includes, as well, cft results for parainfluenza virus and mycoplasma pneumoniae. combining the three methods, a potential causative agent was identified in 56 (90 pe ... | 1996 | 8936958 |
| sequence of echinochloa hoja blanca tenuivirus rna-4. | the sequence is presented of rna-4 of echinochloa hoja blanca tenuivirus (ehbv), one of two tenuiviruses associated with rice cultivation in latin america (together with rice hoja blanca virus; rhbv). analysis of the sequence shows that the coding regions of ehbv rna-4 are closely related to those of rhbv rna-4. however, the intergenic region separating the two ambisense open reading frames, are highly distinct for the two viruses. the features of the rna and the comparisons with the sequences o ... | 1996 | 8938980 |
| sequence of echinochloa hoja blanca tenuivirus rna-3. | analysis of the sequence of the 2336 nucleotide rna-3 of echinochloa hoja blanca tenuivirus shows that it is closely related to rna-3 of rice hoja blanca tenuivirus, the principal virus disease of rice in latin america. this is especially true for the coding regions, where the viruses are almost 90% similar. however, the non-coding regions of rna-3 of these viruses, principally the intergenic region separating the two ambisense open reading frames, are only about 50% similar, suggesting that the ... | 1996 | 8938981 |
| surveillance of community-acquired viral infections due to respiratory viruses in rhone-alpes (france) during winter 1994 to 1995. | nasal swab from patients with acute flu-like illness were evaluated for the presence of respiratory viruses in the rhone-alpes region of france from 1 october 1994 through 2 may 1995. the relative frequencies and seasonal distributions of the specific viruses were assessed. in addition, virus type was correlated with specific clinical signs and symptoms. during the study, 962 samples were collected by 75 medical practitioners participating in the groupe regional d'observation de la grippe survei ... | 1996 | 8940439 |
| reverse transcription-polymerase chain reaction amplification of respiratory syncytial virus genome from neonatal nasal swab samples. | in order to make a rapid and accurate diagnosis of respiratory syncytial virus (rsv) infection, nasal swabs obtained from 14 neonates suspected of having this disease were examined for the presence of rsv genome by reverse transcription (rt) and nested polymerase chain reaction (pcr) amplification, along with enzyme immunoassay (eia), serum neutralization testing and virus isolation. the rt-pcr method was sensitive enough to detect a 0.1 50% tissue culture infectious dose (tcid50) per milliliter ... | 1996 | 8941998 |
| serological study of responses to selected pathogens causing respiratory tract infection in the institutionalized elderly. | in a prospective 2-year study, serological responses to selected pathogens were analyzed in 224 episodes of fever attributable to respiratory tract infection (51.8%) or of unknown source (48.2%) in 131 residents of two long-term-care facilities. a serological response was identified in 45 episodes (20.1%): chlamydia pneumoniae (14 episodes), haemophilus influenzae type b (1), influenza virus type a (14), respiratory syncytial virus (rsv;2), parainfluenza virus type 3 (7), c. pneumoniae and h. in ... | 1996 | 8953065 |
| developmental and environmental factors that enhance binding of bordetella pertussis to human epithelial cells in relation to sudden infant death syndrome (sids). | asymptomatic infection due to bordetella pertussis has been suggested to be one cause of sudden infant death syndrome (sids). we examined developmental and environmental factors previously found to affect binding of another toxigenic species, staphylococcus aureus, to human epithelial cells: expression of the lewis(a) antigen; infection with respiratory syncytial virus (rsv); exposure to cigarette smoke; and the inhibitory effect of breast milk on bacterial binding. binding of two strains of b. ... | 1996 | 8954353 |
| morphological studies of the respiratory syncytial virus induced bronchiolitis in experimentally infected calves. | rsv-infections of the lower respiratory tract in infancy and early childhood are the most frequent causes of a hyperreactive bronchial system and obstructive lung disease. studies concerning the morphological alterations of the bronchial mucosa during an rsv-infection are dependent on an experimental animal model. in this study the alterations of the lower respiratory tract from five infected colostrum-fed calves during the initial stage of the infection are described. brsv strain 375 was applie ... | 1996 | 8958555 |
| safety and immunogenicity of a respiratory syncytial virus subunit vaccine (pfp-2) in ambulatory adults over age 60. | the safety and immunogenicity of purified fusion protein (pfp-2) respiratory syncytial virus (rsv) vaccine was evaluated in a randomized placebo-controlled, double-blind study of 64 healthy adults over age 60. vaccination was well tolerated with no significant acute side-effects. twenty-nine of 33 vaccinees (87%) showed a greater than or equal to fourfold rise in serum igg to the f protein of rsv at 8 weeks post vaccination. twenty of 33 vaccine recipients (61%) had a greater than or equal to fo ... | 1996 | 8961507 |
| phase i study of a recombinant adenovirus-mediated gene transfer in lung cancer patients. | despite vigorous efforts at curbing tobacco consumption and aggressive combined-modality treatment programs, both the incidence of and the mortality from lung cancer have remained virtually unchanged in the last 10 years. more effective innovative therapies are clearly needed. the direct transfer into tumor cells of tumor suppressor genes or toxic gene products that specifically promote tumor cell death and spare nonmalignant cells is a potentially novel anticancer treatment approach that should ... | 1996 | 8961977 |
| [characteristics of anti-respiratory syncytial humoral immunity during persistence of respiratory syncytial virus antigens in adult patients with chronic obstructive bronchitis]. | specific humoral immune response has been studied in 8 patients with chronic obstructive bronchitis, in whom the persistence of rsv was detected, and in a group of patients without rsv persistence. humoral immunity in patients with rsv persistence vs. those with acute and chronic bronchitis without viral persistence was characterized by the following features: a) earlier and more active production of igg and, even more so, igm and iga; b) long persistence of high titers of anti-rsv igg, igm, and ... | 1996 | 8967073 |
| purification of autocrine growth factor from conditioned medium of rat sarcoma (xc) cells. | transformation of rat cells by rous sarcoma virus(es) induced the release of growth factors into serum-free conditioned media. an pr-rsv-transformed rat cell line, xc, produced and released polypeptide factors which promote anchorage-dependent and anchorage-independent growth of xc cells. one of the autocrine factors of xc cells was purified to homogeneity by four-step procedure: ultrafiltration, ion-exchange chromatography on monos, reverse-phase chromatography on spherisorb ods2 and gel filtra ... | 1996 | 8967958 |
| frequency of respiratory syncytial virus in hospitalized infants with lower acute respiratory tract infection in colombia. | in colombia, health workers obtained a nasopharyngeal wash from 103 infants aged less than 12 months hospitalized for acute lower respiratory infection (alri) at the general hospital of medellin during april 1994 to april 1995 so researchers could determine the frequency of alri caused by respiratory syncytial virus (rsv) in hospitalized children. immunofluorescence detected rsv infection in 43 (41.7%) patients. the presence of the following signs and symptoms allowed a clinical diagnosis of a ... | 1996 | 8970224 |
| transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the rela transcription factor as a mechanism producing airway mucosal inflammation. | the most common cause of epidemic pediatric respiratory disease, respiratory syncytial virus (rsv), stimulates interleukin-8 (il-8) synthesis upon infecting airway epithelium, an event necessary for the development of mucosal inflammation. we investigated the mechanism for enhanced il-8 production in human a549 type ii pulmonary epithelial cells. infection with sucrose-purified rsv (prsv) produced a time-dependent increase in the transcriptional initiation rate of the il-8 gene. transient transf ... | 1996 | 8971006 |
| inhibition of viral replication reverses respiratory syncytial virus-induced nf-kappab activation and interleukin-8 gene expression in a549 cells. | previous studies have demonstrated that respiratory syncytial virus (rsv) infection of airway epithelial cells results in the expression of a number of cytokines, such as interleukin-8 (il-8), that are transcriptionally regulated by nuclear factor kappab (nf-kappab). in the studies reported here, we demonstrate that treatment of rsv-infected a549 cells with 100 microg of ribavirin (a viral replication inhibitor) per ml results in reversal of rsv-induced nf-kappab activation, il-8 mrna expression ... | 1996 | 8971048 |
| respiratory syncytial virus-induced cytokine production by neonatal macrophages. | the induction of immunoregulatory cytokines il-1 beta, il-6, il-12, tumour necrosis factor-alpha (tnf-alpha) and interferon-gamma (ifn-gamma) was studied with neonatal (cord blood) monocyte-derived macrophages (mdm) after in vitro infection with respiratory syncytial virus (rsv). the expression of mrnas for these cytokines in rsv-infected mdm was examined by reverse transcriptase-polymerase chain reaction (rt-pcr). the activities of these cytokines were assayed by elisa. significant increase of ... | 1996 | 8973610 |
| update: respiratory syncytial virus activity--united states, 1996-97 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, results in an estimated 90,000 hospitalizations and 4500 deaths each year from lower respiratory tract disease in both infants and young children in the united states. outbreaks occur annually throughout the country. rsv activity in the united states is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes ... | 1996 | 8975116 |
| icam-1 expression and low-molecular-weight g-protein activation of human bronchial epithelial cells (a549) infected with rsv. | the airway epithelial cell of the lower respiratory tract is the primary host target cell for respiratory syncytial virus (rsv) infection. to estimate whether infected epithelial cells contribute to the inflammatory host response observed during the acute infection phase we analyzed the cell surface expression of intercellular adhesion molecule-1 (icam-1, cd54) on human bronchial epithelial cells (a549) following rsv infection and cytokine priming. the epithelial cells constitutively expressed i ... | 1996 | 8975880 |
| comparison of the antiviral efficacy of ribozymes and antisense rna directed against bovine leukemia virus rex/tax. | despite the theoretical attraction of ribozymes, which cleave their target rna, as compared with antisense rna, which only blocks translation, few studies have assessed the relative efficacy of ribozymes and antisense rna directed against the identical target sequence. previously, we described the efficacy of a hammerhead ribozyme targeted against rex/tax, a regulatory gene of bovine leukemia virus (blv). in this study, we asked whether antisense rna targeted against the same site would also be ... | 1996 | 9012866 |
| maturation of respiratory syncytial virus within hep-2 cell cytoplasm. | electron microscopy of hep-2 cells infected with respiratory syncytial virus (rsv) strain long revealed the maturation of rsv on an ultrastructural level. the results showed that the virus maturated by two different pathways. in one of them, the virus assembled and matured before reaching the plasma membrane on the internal vesicle membrane within cytoplasm. the mature virus was delivered to the plasma membrane and to the extracellular space most likely by the transport vesicles and exocytosis. ... | 1996 | 9014011 |
| treatment of respiratory failure due to respiratory syncytial virus pneumonia with natural surfactant. | we describe two infants suffering from severe pneumonia caused by respiratory syncytial virus (rsv) infection and needing mechanical ventilation with both high ventilator settings and a high fraction of inspired oxygen. the severity of the respiratory failure and the possibility of decreased and/or altered surfactant production led us to treat these infants with intratracheal instillation of natural surfactant. this resulted in an improvement of lung compliance and a decrease in the amount of ox ... | 1996 | 9016476 |
| acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (l) gene. | a cold-passaged (cp) temperature-sensitive (ts) mutant of human respiratory syncytial virus designated rsv cpts-248 was previously derived by random chemical mutagenesis of the non-ts mutant cp-rsv that possesses one or more host range mutations. we previously demonstrated in rodents and seronegative chimpanzees that the cpts-248 virus is more attenuated than cp-rsv and is more stable genetically than previously isolated rsv ts mutants. in the present study, we determined that the acquisition of ... | 1996 | 9035372 |
| respiratory syncytial virus infection in the elderly. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections in infants and children throughout the world. respiratory syncytial virus infections in the elderly represent reinfections in the hosts who have had many prior episodes. thus, rsv infections are usually not considered serious in adults, since reinfections are generally known to result in mild disease. nevertheless, in adults, as in children, the infection has been reported to cause altered airway res ... | 1996 | 9072495 |
| dynamic distribution of an antigen involved in differentiation of quail myoblasts transformed with rous sarcoma virus: i. requirement of its quantitative expression on the cell surface for myoblast fusion. | a monoclonal antibody, h-145, the antigen of which is a glycoprotein with a molecular weight of 116 kda, inhibits the fusion of quail skeletal myoblasts transformed with the temperature-sensitive mutant of rous sarcoma virus (ts-rsv) (qm-rsv cells). its antigen shows a unique distribution pattern during myogenic differentiation, and is required continuously for the inhibition as reported previously. the h-145 antigen is expressed from as early as the presumptive myoblast stage, and its expressio ... | 1996 | 9078409 |
| respigam: an rsv immune globulin. | respiratory syncytial virus immune globulin has been shown to prevent or attenuate rsv lower respiratory tract infection in infants under 24 months of age with bpd or who were born prematurely (< or = 35 weeks gestation). the use of rsv-ig is expensive. the estimated average cost of prophylaxis for a season is $4,000 to $5,000 per infant. because of the cost of rsv-ig, and because viral transmission is possible when any blood product is administered, careful consideration of which children shoul ... | 1996 | 9087091 |
| aerosolized immunoglobulin treatment of respiratory syncytial virus infection in infants. | recent studies in animals with experimental respiratory syncytial virus (rsv) infection indicate that passive immunization by intranasal or intratracheal application of gamma-globulins (immunoglobulins) may be beneficial for treatment of infants with lower respiratory tract infection caused by rsv. | 1996 | 8852908 |
| evaluation of relative promoter strengths of the hiv-1-ltr and a chimeric rsv-ltr in t lymphocytic cells and peripheral blood mononuclear cells: promoters for anti-hiv-1 gene therapies. | gene therapy approaches for human immunodeficiency virus type 1 (hiv-1) infections focus on the transfer of critical genetic elements into cd4+ t lymphocytes and cd34+ stem cells. ideally, expression of the anti-hiv-1 gene constructs should be induced during early stages of infection to combat high turnover of the replicating virus. in this study, we investigated the activity of two promoters, hiv-1 long terminal repeat (hiv-1-ltr) and rous sarcoma virus (rsv) ltr fused with the transactivation ... | 1996 | 8854098 |
| growth and induction kinetics of inducible and autoinducible expression of heterologous protein in suspension cultures of recombinant mouse l cell lines. | mouse ltk- cells were transfected with four different plasmids for autoinducible and highly-inducible expression of the bacterial lacz gene and cultivated in suspension. two selection genes, thymidine kinase (tk) and neomycin resistance (neor), were used to select the clones in both cell lines. the resulting two cell lines, designated m4 and r2, differ in that the inducible mmtv promoter from mouse mammary tumor virus (mmtv) controls glucocorticoid receptor (gr) gene and lacz gene expression in ... | 1996 | 8857193 |
| association of rous sarcoma virus dna with xenopus laevis spermatozoa and its transfer to ova through fertilization. | mature xenopus laevis spermatozoa are capable of binding plasmid paprc carrying the complete rous sarcoma virus (rsv) dna. each sperm cell associates, on an average, with 70-160 molecules of the plasmid dna in a dnase resistant form, if the spermatozoa were exposed to the dna at a concentration of 1.0-1.4 micrograms/10(7) sperm cells. fertilization with paprc-treated spermatozoa induced developmental malformations in 25-30% of embryos. immunohistochemical analysis of tissue sections from defecti ... | 1996 | 8858603 |
| expression and characterisation of the ns1 and ns2 proteins of respiratory syncytial virus. | the ns1 and ns2 proteins of human respiratory syncytial virus (rsv) were expressed using baculovirus. antisera to these expressed proteins and to synthetic peptides were raised in rabbits and used to characterise the proteins. multiple forms of both ns1 and ns2 proteins were detected in rsv infected cells by both immunoblotting and radioimmunoprecipitation when non-reducing, but not reducing, conditions were used. in pulse-labelling experiments the monomeric form of ns1 was stable, while that of ... | 1996 | 8864205 |
| respiratory syncytial virus infection in infants: a significant challenge for optimal care. | respiratory syncytial virus (rsv) is a potent pathogen causing annual epidemics of serious illness in young infants. controversy over interventions has evolved given conflicting research results concerning the disease process and the variety of treatment options. investigations over the last two decades have provided more insight into the complexity of this sometimes deadly infection and better understanding of the reasons why treatment is so elusive. currently, rsv infection cannot be prevented ... | 1996 | 8868628 |
| relationship between clinical severity of respiratory syncytial virus infection and subtype. | the relationship between clinical severity of respiratory syncytial virus (rsv) infection and distribution of subtype a or b was investigated. the data of 232 children, who were admitted with rsv infection or diagnosed in the outpatient department of the sophia children's hospital, rotterdam between 1992 and 1995, were studied. the diagnosis of rsv was confirmed by a direct immunofluorescence assay. subtyping was performed by an indirect immunofluorescence assay using specific monoclonal antibod ... | 1996 | 8869195 |
| the product of the v-src-inducible gene nr-13 is a potent anti-apoptotic factor. | tumorigenesis can be induced either by activating cell proliferation or by inhibiting metabolic pathways regulating programmed cell death (apoptosis). there is evidence suggesting that p60(v-src) and other tyrosine kinases protect cells against apoptosis. this effect could contribute to cell transformation by the rous sarcoma virus. mechanism of cell death inhibition by p60(v-src) remains largely unknown. we have recently reported that in avian cells p60(v-src) activates the expression of nr-13, ... | 1996 | 8875982 |
| induction of allergen-specific il-2 responsiveness of lymphocytes after respiratory syncytial virus infection and prediction of onset of recurrent wheezing and bronchial asthma. | in pediatric patients with bronchial asthma and/or atopic dermatitis, peripheral lymphocytes are activated if they are stimulated with the responsible antigen, resulting in induction of responsiveness to il-2. because some nursing infants experience recurrent wheezing after respiratory syncytial virus (rsv) infection, attention is being directed to progression of the disease to bronchial asthma. | 1996 | 8876558 |
| cytolytic activity induced by intramuscular injection of plasmid dna expressing the nucleocapsid protein of the jhm strain of mouse hepatitis virus into c57bl/6 mice. | we constructed plasmids expressing mrna7 coding the nucleocapsid (n) protein of jhm strain of mhv (jhmv) under the control of rous sarcoma virus (rsv) ltr or human elongation factor (ef) 1a promoter, referred to as prsv-mrna7 and pef-mrna7, respectively. although only a slight level of cytolysis was observed by the spleen cells from c57bl/6 mice injected intramuscularly with pef-mrna7, the spleen cells from the mice administered with prsv-mrna7 showed a significant level of cytolytic activities ... | 1996 | 8877970 |
| hypoxanthine-guanine phosphoribosyltransferase (hprt) expression in the central nervous system of hprt-deficient mice following adenoviral-mediated gene transfer. | in this study we show that recombinant adenovirus can augment hypoxanthine-guanine phosphoribosyltransferase (hprt) levels in the central nervous system (cns) of hprt-deficient mice. recombinant adenovirus containing the cdna for rat hprt (rhprt) expressed from the rous sarcoma virus ltr (rsv ltr) was constructed (adrsvrhprt). adrsvrhprt was injected into the right caudate nucleus of 7-week-old hprt-deficient mice. brains were analyzed for gene transfer, transgene expression and function by dna ... | 1996 | 8878108 |
| vitamin a therapy for children with respiratory syncytial virus infection: a multicenter trial in the united states. | high dose vitamin a therapy is effective in reducing morbidity and mortality associated with measles infection. children with acute respiratory syncytial virus (rsv) infection have low serum vitamin a concentrations. | 1996 | 8878220 |
| treatment of respiratory syncytial virus infection with vitamin a: a randomized, placebo-controlled trial in santiago, chile. | treatment with high dose vitamin a reduces complications and duration of hospitalization for children with measles. in respiratory syncytial virus (rsv) infection, as with measles, low serum vitamin a concentrations correlate with increased severity of illness. | 1996 | 8878221 |
| identification of a variant subgroup a strain of respiratory syncytial virus. | during epidemiologic surveillance of children with respiratory syncytial virus (rsv) disease in huntington, w.va., we identified seven strains of a new variant subgroup a rsv (subgroup a-var) by their reactions in an enzyme immunoassay with two anti-f monoclonal antibodies (mabs) specific for two epitopes, f1 and f4, generated against the subgroup b rsv. the prototype strain of subgroup a and all other subgroup a field strains from that epidemiologic year failed to react with these two subgroup ... | 1996 | 8880506 |
| induction of intercellular adhesion molecule-1 in human nasal epithelial cells during respiratory syncytial virus infection. | the effects of infection with respiratory syncytial virus (rsv) on expression of intercellular adhesion molecule (icam)-1 was determined in vitro in nasal epithelial cell cultures. functional consequences of changes in icam-1 expression were assessed by measuring adhesion of a human leukaemic t-cell line to rsv-infected epithelial cells. also, adhesion of phytohaemagglutinin-activated tonsillar lymphocytes (tl) to rsv-infected epithelial cells caused a significant increase in interleukin (il)-4 ... | 1996 | 8881758 |
| construction of human factor ix expression vectors in retroviral vector frames optimized for muscle cells. | development of a highly refined human factor ix (hfix) expression vector system is critical for establishing a durable hemophilia b gene therapy. here we report construction of a series of retroviral vectors and identification of an optimal basic structure and components for expressing hfix in skeletal muscle cells. these vectors, which are derived from moloney murine leukemia virus (momlv) with its enhancer sequence in the 3' long terminal repeat (ltr) deleted, contained internal hfix expressio ... | 1996 | 8886845 |
| generation of atypical pulmonary inflammatory responses in balb/c mice after immunization with the native attachment (g) glycoprotein of respiratory syncytial virus. | the feasibility of using the highly purified native attachment (g) protein in a subunit vaccine against respiratory syncytial virus (rsv) was examined in a murine model with or without the fusion (f) protein of rsv and the adjuvant qs-21. the studies established that qs-21 was more potent than aioh as an adjuvant for both f and g glycoproteins. augmented antigen-dependent killer cell activity and complement-assisted serum neutralizing and anti-f and g protein immunoglobulin g2a antibody titers w ... | 1996 | 8892899 |
| respiratory syncytial virus infection: its role in aeroallergen sensitization during the first two years of life. | our aim was to study the influence of infection with the respiratory syncytial virus (rsv) in non-hospitalized infants on sensitization to aeroallergens and the early manifestation of atopy. six hundred and nine infants from the prospective german multicenter cohort study on atopy were included, 38% of whom had an elevated atopic risk. rsv igg and igm antibodies were tested by elisa with gradient purified rsv antigen. specific ige against mites, cat dandruff, birch and grass pollens and relevant ... | 1996 | 8902854 |
| increased airway reactivity in human rsv bronchiolitis in the guinea pig is not due to increased wall thickness. | bronchiolitis due to the respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infection in the first year of life. it has been suggested that rsv infection may cause subsequent asthma, but a mechanism for this relationship has not been demonstrated. studies examining the presence of airway reactivity in infants with rsv bronchiolitis are limited by our inability to administer provocative agents such as histamine to such ill infants. this makes a small animal mode ... | 1996 | 8905885 |
| heart muscle-specific gene expression using replication defective recombinant adenovirus. | adenoviruses are a promising vector system for future gene therapy of heart muscle diseases. the promiscuous tissue tropism of adenoviruses, however, may lead to the undesirable expression of putative therapeutic genes in nontarget cells and hence to considerable safety limitations for this vector system. to restrict gene expression to cardiomyocytes we constructed an adenoviral vector (ad-mlcluc) in which the luciferase gene is under the control of the ventricle-specific myosin light chain-2 (m ... | 1996 | 8908506 |
| phase i study of intravenous ribavirin treatment of respiratory syncytial virus pneumonia after marrow transplantation. | respiratory syncytial virus (rsv) pneumonia in marrow transplant recipients is associated with significant mortality. ribavirin is a nucleoside analog with activity against rsv and in its aerosolized formulation is the only drug approved for treatment of rsv pneumonia in the united states. the clinical use of aerosolized ribavirin has been limited by caregivers' concerns about drug exposure and potential teratogenic effects. since there is lack of proven efficacy and safety of the aerosolized ri ... | 1996 | 8913463 |
| nucleotide sequence analysis of the respiratory syncytial virus subgroup a cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate. | the complete nucleotide sequence of the rsv cpts-248/404 live attenuated vaccine candidate was determined from cloned cdna and was compared to that of the rsv a2/hek7 wild-type, cold-passaged cp-rsv, and cpts-248 virus, which constitute the series of progenitor viruses. rsv cpts-248/404 is more attenuated and more temperature sensitive (ts) (shut-off temperature 36 degrees) than its cpts-248 parent virus (shut-off temperature 38 degrees) and is currently being evaluated in phase i clinical trial ... | 1996 | 8918930 |
| in vitro assessment of variables affecting the efficiency and efficacy of adenovirus-mediated gene transfer to cystic fibrosis airway epithelia. | primary cultures of airway epithelia were used to evaluate variables pertinent to adenovirus (ad)-mediated gene transfer efficiency and efficacy including: (i) ad-vectors with different promoters, (ii) the duration of vector incubation with cells, (iii) the concentration and depth of vector-containing medium at constant multiplicity of infection (moi) (10(3)), and (iv) the relative sensitivity of reverse transcription polymerase chain reaction (rt-pcr) versus functional analysis for the detectio ... | 1996 | 8825868 |
| immunological determinants of disease caused by respiratory syncytial virus. | various disease syndromes caused by respiratory syncytial virus (rsv) may have a common mechanism of pathogenesis mediated by cytokines produced by type 2 t helper cells. the nature of the immune response to rsv is determined by the pattern of cytokines produced sequentially by many different cell types. vaccination can influence the types of cytokine produced by selectively activating t cell subpopulations and inducing an immune response that clears the virus with minimal immunopathology. | 1996 | 8829339 |
| cytologic manifestations of respiratory syncytial virus pneumonia in bronchoalveolar lavage fluid: a case report. | respiratory syncytial virus (rsv) pneumonia in immunocompromised patients, especially bone marrow transplant recipients, is associated with high mortality. early diagnosis in these cases is important because antiviral therapy with ribavirin is effective in reducing mortality. | 1996 | 8669193 |
| combination antiviral therapy for respiratory virus infections. | a limited number of antiviral drug combinations have been shown to have enhanced activity for important human respiratory viruses. rimantadine or amantadine combined with ribavirin shows increased antiviral effects in vitro and in experimental animal models. this combination warrants testing in human influenza. immunoglobulin containing neutralizing anti-rsv antibody combined with ribavirin shows enhanced antiviral effects in experimental animal infections and provides clinical benefit in severe ... | 1996 | 8721543 |
| community respiratory virus infections among hospitalized adult bone marrow transplant recipients. | from 1 november 1992 through 1 may 1993 and from 1 november 1993 through 1 may 1994, we conducted a prospective surveillance study at the university of texas m.d. anderson cancer center (houston) to evaluate the role of community respiratory virus infections in hospitalized adult bone marrow transplant (bmt) recipients, respiratory secretions were obtained from all adult bmt recipients with acute respiratory illnesses. during these two winters, a community respiratory virus was isolated from 37 ... | 1996 | 8722930 |
| further investigation of some inhibitors on myogenic differentiation: mechanism of inhibition with hmba on quail myoblasts transformed with rous sarcoma virus. | to investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (qm cells) transformed with a temperature-sensitive mutant of rous sarcoma virus (ts-rsv), termed qm-rsv cells. at 35.5 degrees c, a permissive temperature for rsv, qm-rsv cells repeatedly proliferate without differentiation, but, at 41 degrees c, a nonpermissive temperature, myogenic differentiation proceeds. this temperature dependency of the differentiation is derived from protein kinase activity of pp ... | 1996 | 8726470 |
| systemic and portal vein delivery of human kallikrein gene reduces blood pressure in hypertensive rats. | there is an inverse correlation between systemic blood pressure and urinary kallikrein levels in humans and hypertensive animal models, suggesting that the tissue kallikrein-kinin system plays an important role in blood pressure regulation. in this study, we explored the potential of human kallikrein gene delivery on blood pressure reduction in spontaneously hypertensive rats (shr). the human tissue kallikrein gene or cdna was placed under the control of following promoters: the metallothionein ... | 1996 | 8727504 |
| peptides from conserved regions of paramyxovirus fusion (f) proteins are potent inhibitors of viral fusion. | the synthetic peptides dp-107 and dp-178 (t-20), derived from separate domains within the human immunodeficiency virus type 1 (hiv-1) transmembrane (tm) protein, gp4l, are stable and potent inhibitors of hiv-1 infection and fusion. using a computer searching strategy (computerized antiviral searching technology, c.a.s.t.) based on the predicted secondary structure of dp-107 and dp-178 (t-20), we have identified conserved heptad repeat domains analogous to the dp-107 and dp-178 regions of hiv-1 g ... | 1996 | 8700906 |
| respiratory syncytial virus infections in pediatric renal transplant recipients. | immunocompromised patients are considered at increased risk from respiratory syncytial virus (rsv) infection. we examined the incidence and outcome of rsv infection in pediatric renal transplant (tx) recipients on chronic immunosuppressive therapy. of 173 recipients transplanted between november 1985 and april 1993, 5 (3%) developed rsv infection (age range 11-39 months). initial immunosuppression included prednisone, azathioprine, cyclosporine, and polyclonal antibody therapy. time from tx to o ... | 1996 | 8703717 |
| neutralizing and enhancing activities of human respiratory syncytial virus-specific antibodies. | the neutralizing and enhancing activities of respiratory syncytial virus (rsv)-specific antibodies were examined. these two biological activities were measured for a panel of six monoclonal antibodies (mabs) specific to the rsv surface f and g glycoproteins. four mabs specific for the f protein possessed both neutralizing and enhancing activities. one mab (11-2-d2), specific to the g protein, enhanced rsv infection of u937 cells, a human macrophage cell line, but did not neutralize virus infecti ... | 1996 | 8705669 |
| surface display on staphylococci: a comparative study. | two different host-vector expression systems, designed for cell surface display of heterologous receptors on staphylococcus xylosus and staphylococcus carnosus, respectively, were compared for the surface display of four variants of a 101 amino acid region derived from the g glycoprotein of human respiratory syncytial virus (rsv). surface localization of the different chimeric receptors was evaluated by a colorimetric assay and by fluorescence-activated cell sorting. it was concluded that the s. ... | 1996 | 8706888 |
| the structurally diverse intergenic regions of respiratory syncytial virus do not modulate sequential transcription by a dicistronic minigenome. | the first nine genes of respiratory syncytial virus (rsv), a nonsegmented negative-strand rna virus, are separated by intergenic regions which range in size from 1 to 52 nucleotides for strain a2 and lack obvious consensus elements except that each ends in an a (genome sense). their significance for gene expression was investigated by using rsv-cat-luc rna, a helper-dependent cdna-encoded dicistronic analog of rsv genomic rna in which the viral genes were replaced by a negative-sense copy of the ... | 1996 | 8709239 |
| dexamethasone in bronchiolitis: a randomised controlled trial. | although corticosteroids are commonly prescribed in the treatment of bronchiolitis, there is no evidence on the efficacy of these drugs in this disorder. we designed a randomised, double-blind, prospective study to assess the efficacy of dexamethasone in infants with bronchiolitis who require hospital management. | 1996 | 8709687 |
| adenovirus-mediated thymidine kinase gene transduction in human epithelial ovarian cancer cell lines followed by exposure to ganciclovir. | in an effort to develop gene therapy for ovarian cancer efficacy and toxicity of adenovirus-mediated transfer of the hsv-tk gene followed by administration of ganciclovir were studied in two human epithelial ovarian cancer cell lines ov-ca-2774 and ov-ca-1225. 100% transduction was achieved in both cell lines at mois of 7 and 15 as demonstrated by x-gal staining. no toxicity of virus alone was observed at mois up to 30. gcv was not toxic up to 200 micrograms/ml. cell killing efficacy was shown t ... | 1996 | 8712678 |
| discrimination of respiratory syncytial virus subgroups a and b by reverse transcription-pcr. | reverse transcription (rt)-pcr with shared primers differentiating respiratory syncytial virus (rsv) subgroups a and b was developed for subtyping of rsv isolates. results of rt-pcr were compared with those of an indirect immunofluorescence test using monoclonal antibodies. viral rna isolated from cell cultures infected with rsv served as a template for cdna synthesis with random primers. for pcr, we used three synthetic oligonucleotides corresponding to the g protein mrna sequence of subgroup a ... | 1996 | 8748269 |
| isolation of a neutralizing human rsv antibody from a dominant, non-neutralizing immune repertoire by epitope-blocked panning. | we isolated a large panel of human abs directed against the respiratory syncytial virus (rsv) ag from combinatorial phage display libraries. following initial differentiation of the fabs by bstni restriction patterns, dna sequence analysis revealed 10 different classes of vh paired with more than 35 different vl genes. all the fabs bound with high affinity to the f ag. however, most fabs competed with the binding of a representative member of this group, suggesting that the fabs recognized a com ... | 1996 | 8752928 |
| respiratory syncytial virus infection and meningococcal disease. | although viral respiratory tract infections may predispose to meningococcal disease, strong evidence that they do so exists only for influenza. data on laboratory reported cases of respiratory syncytial virus (rsv) infections and meningococcal disease in england and wales from mid-1989 to mid-1994 were analysed. although the rise in rsv cases preceded the rise in meningococcal disease cases each winter, the interval between the rise and fall of the two diseases was inconsistent, no association w ... | 1996 | 8760957 |