Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [frequency of enteropathogenic micro-organisms isolated in children with and without acute diarrhea]. | 1985 | 2861829 | |
| seroepidemiology of adult rotavirus. | 1985 | 2862483 | |
| [etiology of acute infantile gastroenteritis in gabon]. | rotaviruses are the main etiology of acute diarrhoeas in gabonese children (11 to 30% according to age). salmonellae (11.4%), shigellae (7.1%) and e. histolytica (7.1%), isolated or associated with enterobacteria, e. coli (3%), giardia and strongyloides stercoralis (1.4%), yersinia enterocolitica (1%) and balantidium coli (0.5%) were also found, without cholera. | 1985 | 2863005 |
| rotavirus-like agent, rats, and man. | 1985 | 2864595 | |
| chinese adult rotavirus is a group b rotavirus. | 1985 | 2865590 | |
| the relative importance of enteric pathogens affecting neonates of domestic animals. | 1985 | 2868628 | |
| isolation and characterization of cytopathic strains of rotavirus from rabbits. brief report. | three cytopathic rotavirus isolates were recovered from young rabbits affected by an enteric syndrome. the three isolates, when compared by cross serum-neutralization tests, were found to be of the same serotype. cross neutralization occurred also between a representative of the rabbit isolates and one strain of bovine rotavirus. | 1985 | 2982351 |
| a two-year longitudinal study on the etiology of acute diarrhea in young children in northern italy. | during a 24-month period, 561 young children (6 months-2 years of age) hospitalized for acute diarrhea were studied for enteric pathogens. patients positive for one or more pathogens were 359 (64.0%). infection with one pathogen was found in 266 (47.4%) patients, whereas multiple infection was detected in 93 (16.6%) patients. enteropathogens associated with disease were rotaviruses: 150 (26.7%), adenoviruses: 99 (17,6%), non-polio enteroviruses: 48 (8.5%), coronaviruses: 10 (1.8%), parvoviruses: ... | 1985 | 2983179 |
| rotavirus infection in gastroenteritis in children. | the electron microscopic investigation of biological materials (faeces, intestinal mucous biopsies) from 19 children and sucklings with clinically diagnosed acute gastroenteritis evidenced the presence of some rotavirus-like viral particles in 31 percent of cases. the age of hospitalized subjects varied between 4 months and three years. there have been identified viral particles in the cytoplasm of enterocytes of the intestinal villi and in suspensions of the fecal materials. | 1985 | 2931592 |
| epidemiological model of acute bacterial and viral diarrhoeal diseases. | 1985 | 2933447 | |
| evidence of immunity induced by naturally acquired rotavirus and norwalk virus infection on two remote panamanian islands. | for better understanding of the role of humoral immunity in ameliorating infections with rotavirus (rv) and norwalk virus (nw), 305 cuna indians living on two isolated islands located off panama's carribean coast were surveyed daily for diarrhea over a seven-month period. nine (8%) of 108 persons with a baseline rv antibody titer of greater than 1:4 developed rv infection compared with 70 (46%) of 151 persons with a baseline rv antibody titer of less than 1:4 (p less than .001). thirty-eight (25 ... | 1985 | 2981278 |
| serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections. | nineteen rotavirus strains derived from asymptomatic neonates (seven from england, five from australia, two from venezuela, and five from sweden) were successfully cultivated in primary african green monkey kidney cell cultures, serotyped by plaque reduction neutralization tests, subgrouped by indirect enzyme-linked immunosorbent assay, and electropherotyped by polyacrylamide gel electrophoresis. all 19 strains were shown to fall into one of the four known human serotypes; serotype 1 (all venezu ... | 1985 | 2984247 |
| isolation and identification of caliciviruses from dogs with enteric infections. | caliciviruses were isolated from 7 dogs and 1 captured coyote with enteritis. there was a high fatality rate in dogs 4 to 16 weeks of age. the occurrence in these dogs of concurrent infection with known enteric pathogens such as salmonella sp, canine parvovirus, canine coronavirus, and canine rotavirus did not allow making any conclusions regarding the pathogenicity of this newly recognized calicivirus. the caliciviruses were characterized by electron microscopy and were further identified as be ... | 1985 | 2982299 |
| rotaviral and coronaviral diarrhea. | a number of different viruses can be primary pathogens in the neonatal calf diarrhea complex. by far the most common viruses causing calfhood diarrhea found throughout the world are rotaviruses and coronaviruses. primary infection of newborn calves with either one of these viruses can cause severe intestinal alterations and diarrhea. rotaviruses can produce high-morbidity outbreaks of diarrhea in calves under 10 days of age. morality is variable mainly owing to secondary bacterial infections and ... | 1985 | 3000544 |
| [biochemical study of rotavirus strains isolated in new caledonia between 1980 and 1983. electrophoretypes]. | between 1980 and 1983, 150 rotavirus strains have been identified by elisa test. rna was extracted from 90 strains. we used a very simple and rapid extraction technique. polyacrylamide gel electrophoresis permits the separation of double stranded rna segments. the system of classification we retained, allows to recognize 8 classes of electrophoretypes. this study confirms the great variability of human rotaviruses. | 1985 | 3000639 |
| [epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics]. | diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ... | 1985 | 3000920 |
| effect of relative humidity, atmospheric temperature, and suspending medium on the airborne survival of human rotavirus. | the wa strain of human rotavirus, grown in ma-104 cells, was suspended either in tryptose phosphate broth or feces from a case of rotaviral diarrhea. it was then aerosolized into a rotating drum using a collison nebulizer. the drum air was sampled using an all-glass impinger containing tryptose phosphate broth as collecting fluid. at 20 +/- 1 degree c, the virus aerosolized from tryptose phosphate broth was found to survive best at 50 +/- 5% relative humidity, where its half-life was 44.2 +/- 6. ... | 1985 | 3000566 |
| direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests. | during february and march 1984, 207 fecal samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (rotazyme; abbott laboratories, north chicago, ill., and enzygnost-rotavirus; calbiochem-behring, la jolla, calif.) and two latex agglutination tests (rotalex; orion research, inc., cambridge, mass., and slidex rota-kit; biomérieux). all stool samples were also tested for yeasts and bacterial pathogens. electron microscopy was us ... | 1985 | 2985650 |
| development of a sensitive protein a-gold immunoelectron microscopy method for detecting viral antigens in fluid specimens. | protein a-colloidal gold immunoelectron microscopy (pag iem) has been employed to specifically detect rotavirus and enterovirus antigen in negatively stained fluid specimens. unlike other iem methods, pag iem can detect not only viral antigen associated with morphologically recognizable particles but also viral antigens of unrecognizable ultrastructure. this rapid and sensitive immunoassay was found to be applicable to virus-infected stool specimens as well as partially purified virus preparatio ... | 1985 | 3001115 |
| use of immunosorbent electron microscopy for detection of rota- and hepatitis a virus in sucrose solutions. | immunosorbent electron microscopy was used to demonstrate rotavirus in solutions of varying sucrose concentrations after 18, 42 and 66 h of incubation. about 50% of adsorption of virus particles to the grid was achieved after 18 h incubation and nearly 100% after 42 h when compared to trapping of virus from sucrose free solutions. hepatitis a virus was purified in a 10-30% sucrose gradient and each fraction was examined by immunosorbent electron microscopy, direct electron microscopy, immune ele ... | 1985 | 3001117 |
| detection of rotavirus by latex agglutination. | 1985 | 3001154 | |
| pathogens in fecal samples from apparently healthy children. | 1985 | 3001380 | |
| significance of e. coli and rotavirus in infantile diarrhoea. | 1985 | 3001381 | |
| effect of age and malnutrition on rotavirus infection in mice. | litters of malnourished and normally nourished suckling mice of various ages were fed mouse rotavirus (10(5) id50). infection was monitored by immunofluorescent staining of isolated epithelial enterocytes 24, 28, and 72 h after virus ingestion; enzyme linked immunosorbent assay of daily fecal samples; and determining the severity of diarrhea by noting the color and consistency of the feces and the presence or absence of staining. in malnourished mice 5 to 9 days old, all the parameters of infect ... | 1985 | 3001627 |
| dose-response study of rit 4237 oral rotavirus vaccine in breast-fed and formula-fed infants. | the rit 4237 live attenuated bovine rotavirus vaccine was given orally at three dose levels to 75 breast-fed, 40 formula-fed and 24 fasting infants ages 4 to 6 months. vaccine of 10(8.3) (50% tissue culture-infective doses) (tcid50) per dose gave a neutralizing antibody response in 14 of 14 (100%) formula-fed, in 18 of 26 (69%) breast-fed and in 5 of 8 (63%) fasting infants, or an overall response rate of 77% (37 of 48). the overall response rate to a vaccine of 10(7.2) tcid50 per dose was 61% ( ... | 1985 | 3001659 |
| diverse serologic response to rotavirus infection of infants in a single epidemic. | eight infants with onset of gastroenteritis occurring within a 32-day period were examined for stool rotavirus antigen and serum antibody response to rotavirus. each infant presented evidence of rotavirus infection but no single test for rotavirus-specific antigen or antibody was positive for every infected individual. rotavirus was identified in the stools of five infants; in the others rotavirus etiology was determined by a specific immune response only. the neutralizing antibody response to e ... | 1985 | 3001660 |
| rotavirus rna variation during chronic infection of immunocompromised children. | polyacrylamide gel electrophoresis was performed on rna from rotaviruses isolated from two immunocompromised patients with prolonged rotavirus diarrhea. extensive variations were observed in the genomes of rotaviruses isolated from each patient during a 16-week period of their illness. an animal model of rotavirus infection was used to help evaluate the results found in our patients. individual mouse rotavirus strains were found to be stable during serial passage, but individual animals could be ... | 1985 | 3001661 |
| independent segregation of two antigenic specificities (vp3 and vp7) involved in neutralization of rotavirus infectivity. | antiserum prepared against the m37 strain of rotavirus, recovered from an asymptomatic newborn infant in venezuela, neutralized two prototype human rotaviruses that define two separate serotypes: serotype 1 (wa) and serotype 4 (st3). thus, the m37 strain is a naturally occurring intertypic rotavirus. analysis of reassortant viruses produced during coinfection in vitro indicated that the observed dual serotype specificity of m37 resulted from sharing a related outer capsid protein, vp3, with the ... | 1985 | 3001716 |
| [rotavirus and pararotavirus infections in adults. analysis of a nosocomial infection and some sporadic cases]. | a nosocomial infection in adults due to rotavirus is described. the identity of the infecting rotavirus strains was demonstrated by genomic dsrna electrophoresis. at an interval of 19 days another patient in the same hospital developed a pararotavirus infection. pararotaviruses or rotavirus-like agents are antigenetically distinct from rotaviruses and thus cannot be detected at present by the available antibody mediated antigen detection systems. the current prevalence of pararotavirus infection ... | 1985 | 3001931 |
| rotavirus infections and their prevention by vaccination. | 1985 | 3002263 | |
| [increased knowledge about viruses as a cause of diarrhea. the protective effect of rotavirus vaccine is tested now]. | 1985 | 3003471 | |
| the need for new antiviral agents. | population density and immune status, vectors and virulence of infection, nutritional status, sanitation, genetic susceptibility and medical management of cases, are important factors influencing the incidence and/or severity of virus infections. thus, the prevalence and clinical importance of virus infections and the need for antiviral drugs differ from place to place and from time to time. national and world health statistics of notifications of disease give some index of the incidence of infe ... | 1985 | 3004326 |
| [serotyping of cytopathic rotavirus strains isolated from calves]. | antistrain sera were obtained against cytopathic rotavirus strains isolated from calves as well as against reference rotavirus strains, belonging to i and ii serotype of the bovine rotaviruses with which cross antigenic studies were carried out. it was found that the isolated ds 39/82 and tr 248/82 strains belonged to i serotype of the bovine rotaviruses. data was obtained speaking of some minor antigenic differences between strain tr 248/82 and strain ds 39/82 as well as between strain tr 248/8 ... | 1985 | 3002007 |
| rotavirus infections in newborns: an epidemiological and clinical study. | an outbreak of rotavirus infections among newborns at karolinska hospital, stockholm, which has been going on for greater than 2 years has been followed with clinical and epidemiological investigations. about one third of the babies born in the hospital were infected at the age of 3 days. the clinical symptoms were mild, 8.8% of the rotavirus positive babies had loose stools compared to 1.9% of those who did not excrete the virus. an epidemiological survey in the neonatal intensive care unit sug ... | 1985 | 3003889 |
| [epidemiology of rota-virus gastro-enteritis]. | 1985 | 3004737 | |
| [analysis of the genome of rotavirus from the feces of adult infectious diarrhea]. | 1985 | 3004767 | |
| aetiological forms. | at least 25 etiologic agents have been implicated in acute diarrhea. in children, the most common are the rotaviruses, enterotoxigenic escherichia coli, campylobacter jejuni, salmonallae, and shigellae. for example, an attempt was made to isolate enteropathogenic micro-organisms in children under 2 years of age hospitalized in ethiopia with severe or moderate dehydration. a causal agent was identified in 70% of the 175 cases analyzed. 49% of these cases involved rotavirus infections, 19% inc ... | 1985 | 12314104 |
| the gambia and bangladesh: the seasons and diarrhoea. | climactic factors in the gambia and bangladesh have an important impact on the incidence of diarrheal disease. both countries share some common characteristics in climate, including a cool dry winter of 3 months followed by a hot dry spring and hot wet summers of 5-7 months in length. the main difference is in the amount of rainfall. the gambia may have 20-30 inches of rain each year; bangladesh usually has up to 4-5 times this amount. in the gambia, drought is a recurring problem; floods is ... | 1986 | 12315285 |
| electrophoretic variation of avian rotavirus rna in polyacrylamide gels. | five different sorts of rna profiles (electropherogroups 1 to 5) were recognised when avian rotavirus rnas were electrophoresed in polyacrylamide gels. these could be distinguished from the rnas of mammalian group a, b and c rotaviruses. viruses belonging to electropherogroups 1, 2/3 and 4 were detected in chickens and viruses belonging to electropherogroups 1, 2, 3 and 5 were detected in turkeys. a minimum of three electropherogroups was recognised in each of four longitudinal surveys of broile ... | 1986 | 18766513 |
| pathogenesis of rotavirus infection in turkey poults. | the pathogenesis of rotavirus infection was examined after experimental infection of conventional and specific-pathogen-free (spf) turkey poults. in six experiments birds were exposed to turkey rotavirus isolates tu-1 or tu-2 or the chicken isolate ch-1 at 7, 10 or 42 days of age. poults were examined between 1 and 24 days post-infection (dpi) for diarrhoea, gross and histopathologic lesions, virus excretion in the intestinal tract, viral antigen in intestinal epithelial cells, and the developme ... | 1986 | 18766543 |
| cytopathic avian rotavirus isolated from duck faeces in chicken kidney cell cultures. | five cytopathic viruses morphologically resembling rotaviruses were isolated from duck faeces in chicken kidney cell cultures using conventional methods. one of the isolates, designated f-29 strain, was identified as an avian rotavirus from the following: ribonucleic acid in the viral core, virus growth in the cytoplasm, resistance to chloroform, acid and heating, partial stabilisation to molar magnesium chloride and rotavirus-like morphology by electron microscopy. by immunofluorescence, f-29 s ... | 1986 | 18766573 |
| molecular basis of rotavirus virulence: role of gene segment 4. | bovine rotavirus ncdv and simian rotavirus sa-11 exhibited markedly different patterns of gastrointestinal tract disease when inoculated orally into newborn mice. a genetic approach was used to define the molecular basis of these differences. the sa-11 strain of rotavirus was more virulent than the ncdv strain when inoculated orally into newborn mice; the dose of sa-11 required to cause diarrhea in 50% of infant mice was 50-fold less than that required for ncdv. nineteen reassortant viruses were ... | 1986 | 3001364 |
| rotavirus: the major etiologic agent of severe infantile diarrhea may be controllable by a "jennerian" approach to vaccination. | 1986 | 3009632 | |
| evaluation of rhesus rotavirus vaccine (mmu 18006) in infants and young children. | orally administered rhesus rotavirus vaccine was evaluated in a placebo-controlled study in young children and infants (ages, eight months to 61 months). thirteen children received the rotavirus vaccine, and ten children served as the control group. the vaccine was well tolerated. there were no significant differences between the vaccine recipients and the control group in the number of child-days with temperatures greater than or equal to 37.8 c, vomiting, diarrhea, or cough. there were signifi ... | 1986 | 3009633 |
| heterogeneity in base sequence among different dna clones containing equivalent sequences of rotavirus double-stranded rna. | the nucleotide sequences for several complementary dna clones of the rotavirus genome were determined. when the sequences obtained from different clones for the same regions (16,000 bases) were compared, differences in eight base positions were observed. these discrepancies, approximately 1 in 2,000 bases, may be due to differences in individual rna genomes resulting from multiple passages; infidelity of dna synthesis in the cloning procedure; or both factors. whatever the cause, this frequency ... | 1986 | 3005622 |
| comparison of five methods for detecting human rotavirus in stool specimens. | 1986 | 3007153 | |
| epidemiology of rotavirus in guayaquil, ecuador. | detection of rotavirus by electron microscopy was conducted with fecal specimens from 1,722 infants and young children with acute diarrhea, during a 41-month survey from april 1978 through december 1981 in guayaquil, ecuador; 376 of these specimens (21.8%) were positive. the detection rate was higher during the dry season (may to november; 25.2%) than during the rainy season (december to april; 14.7%). when rotaviruses isolated from 59 patients hospitalized with diarrhea (from april 1979 to july ... | 1986 | 3006530 |
| inactivation of human and simian rotaviruses by chlorine. | the inactivation of simian rotavirus sa-11 and human rotavirus type 2 (wa) by chlorine was compared at 4 degrees c by using single-particle virus stocks. both virus types were usually more readily inactivated at ph 6.0 than at ph 8.0 when low chlorine concentrations (0.05 to 0.2 mg/liter) were used. a complete (5 log) reduction of both was obtained within 20 s at all ph levels when chlorine concentrations were increased to 0.3 mg/liter. slight differences in the chlorine sensitivities of sa-11 a ... | 1986 | 3006589 |
| genetic relatedness among animal rotaviruses. | the genomic relatedness among representative rotavirus strains was examined by employing cross-hybridization techniques. single stranded (ss) rna prepared by in vitro transcription of purified rotavirus particles and labeled with either 32p or 125i was hybridized to denatured genomic, double stranded (ds) rnas. the hybrids formed were analyzed by polyacrylamide gel electrophoresis (page) or by testing their sensitivity to digestion with single strand specific nuclease (s-1 nuclease). a relativel ... | 1986 | 3004389 |
| atypical rotavirus from south african neonates. brief report. | rotaviruses displaying an rna profile different from other human rotaviruses were detected in stools from six healthy neonates. these viruses shared the common group a antigen, unlike most other atypical human rotavirus electropherotypes described to date. | 1986 | 3004392 |
| comparative efficacy of broad-spectrum antiviral agents as inhibitors of rotavirus replication in vitro. | several nucleoside analogues which have previously been established as broad-spectrum antiviral agents, i.e. ribavirin, vidarabine, pyrazofurin, tubercidin, carbodine, (s)-9-(2,3-dihydroxypropyl)adenine [(s)-dhpa], carbocyclic 3-deazaadenosine (c-c3 ado), (rs)-3-adenine-9-yl-2-hydroxypropanoic acid [(rs)-ahpa] isobutyl ester and neplanocin a were compared for their potency and selectivity as inhibitors of human rotavirus (strains wa, kun and mo) replication in vitro. as the most efficacious inhi ... | 1986 | 3008646 |
| coproantibody response to rotavirus in an outbreak in a day-care nursery. | 1986 | 3007154 | |
| in vitro transcription of human pararotavirus. | purified human pararotavirus obtained from stool samples from a 6-month-old infant was characterized. electron microscopy of the viral particles subjected to different treatments suggested that the protein shells differed from those described for rotavirus. treatment with both edta or ethylene glycol-bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid in the presence or absence of mg2+ seemed to convert the virions into core particles by removal of both the outer and inner shells, and no parti ... | 1986 | 3001343 |
| rapid dot-immunobinding assay on nitrocellulose for viral antibodies. | a procedure is described for the routine laboratory diagnosis of viral serum antibodies. antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. the test allows processing of multiple sera in one 3- to 5-h operation and is equal to or more sensitive than serum neutralization, hemagglutination inhibition, and fluores ... | 1986 | 3009525 |
| simultaneous infections with different enteric and respiratory tract viruses. | infants and young children with rotavirus (rv) or visualized adenovirus in their stools were tested for the simultaneous presence of a respiratory viral pathogen in their upper respiratory tract. overall, at least 10.7% of 484 study subjects had such dual infections, including 8.3% of 385 rv-positive gastroenteritis patients and 24.3% of 37 rv-positive respiratory disease patients. respiratory syncytial virus was present in 34.1% of 41 dual infections with rv and at least 40% of the 12 to 15 dua ... | 1986 | 3009527 |
| detection of rotavirus with a new polyclonal antibody enzyme immunoassay (rotazyme ii) and a commercial latex agglutination test (rotalex): comparison with a monoclonal antibody enzyme immunoassay. | a total of 176 human fecal specimens were examined for the presence of rotavirus by four different assays: a monoclonal antibody enzyme immunoassay; the original polyclonal antibody enzyme immunoassay marketed by abbott laboratories, north chicago, ill. (rotazyme i); a modification of this assay which is now commercially available (rotazyme ii); and a latex agglutination test (rotalex) recently introduced by medical technology corp., somerset, n.j. in addition, selected specimens were examined f ... | 1986 | 3009531 |
| comparison of immune electron microscopy and genome electropherotyping techniques for detection of turkey rotaviruses and rotaviruslike viruses in intestinal contents. | seventy-nine intestinal contents specimens from 65 turkey flocks were examined for rotavirus and rotaviruslike virus (rvlv) by immune electron microscopy (iem) and genome electropherotyping. the iem procedure was slightly more sensitive in detecting these viruses; 7 of 48 specimens (14.6%) positive for virus by iem were negative by the genome electropherotyping technique. the genome electropherotyping technique more readily differentiated the rotaviruses and rvlvs than did the iem procedure; 15 ... | 1986 | 3009539 |
| group c rotaviruses in humans. | atypical rotaviruses obtained from human feces from australia, brazil, and the united kingdom were shown by a combination of techniques--immunoelectron microscopy, immunofluorescence, genome profile analysis, terminal fingerprint analysis of genome segments, and dot-blot hybridization--to be related to group c porcine rotaviruses. the prevalence of antibody to group c rotaviruses was found to be low in human sera and immunoglobulin pools from six countries. no signs of infection were obtained wh ... | 1986 | 3009541 |
| other viruses with etiologic roles in childhood gastroenteritis. | rotaviruses and norwalk-like viruses are the two groups of viruses most frequently associated with gastroenteritis, but as outlined in this review several other viral agents have also been associated with acute gastroenteritis. the gastroenteritis viruses are generally fastidious, and thus traditional cell culture isolation and detection procedures are not applicable; therefore electron microscopy and immunoelectron microscopy remain among the most powerful techniques for studying these viruses. ... | 1986 | 3003721 |
| the role of rotaviruses in pediatric diarrhea. | although rotavirus has been recognized as the most common etiologic agent of gastroenteritis in infants requiring hospitalization, there are several important gaps in the understanding of rotavirus infection. obstacles to such an understanding have included difficulties in cultivating the virus from human stools, the lack of a simple animal model to examine the immune response, and differences in the epidemiology of the infection in developed vs. developing countries. there is a strong need fo ... | 1986 | 3003719 |
| identification of the two rotavirus genes determining neutralization specificities. | bovine rotavirus ncdv and simian rotavirus sa-11 represent two distinct rotavirus serotypes. a genetic approach was used to determine which viral gene segments segregated with serotype-specific viral neutralization. there were 16 reassortant rotaviruses derived by coinfection of ma-104 cells in vitro with the sa-11 and ncdv strains. the parental origin of reassortant rotavirus double-stranded rna segments was determined by gene segment mobility in polyacrylamide gels and by hybridization with ra ... | 1986 | 3001359 |
| definition of two new groups of atypical rotaviruses. | comparative antigenic and nuclei acid analyses were carried out on two new atypical rotavirus isolates coming respectively from chickens (d/132) and pigs (e/dc-9). indirect immunofluorescence showed that each virus carried different group antigens which were also distinct from those of previously described rotavirus groups. by genome profile analysis each virus had a pattern of genomic rnas clearly distinct from those of the other rotavirus groups. comparative terminal fingerprinting of correspo ... | 1986 | 3003232 |
| safety, infectivity, transmissibility and immunogenicity of rhesus rotavirus vaccine (mmu 18006) in infants. | in an attempt to evaluate the immunogenicity, infectivity, transmissibility and safety of rhesus rotavirus vaccine (rrv) mmu 18006, 27 infants ages 5 to 20 months participated in two randomized, double-blind placebo controlled trials, one in a day care setting to allow for child to child contact and close surveillance and the other on an outpatient basis. fourteen infants (mean age, 8.3 months) received 10(5) plaque-forming units of rrv and 13 (mean age, 11.1 months) received placebo. in the eig ... | 1986 | 3003715 |
| reactivity patterns to human rotavirus strains of a monoclonal antibody against vp2, a component of the inner capsid of rotavirus. brief report. | a non-neutralizing monoclonal antibody (yo-60) against human rotavirus was found to be directed to vp2 (90,000-dalton protein), one of the two major components of the inner capsid. the reactivity patterns of the yo-60 antibody were very similar, though not identical, to those of subgroup ii-specific yo-5 monoclonal antibody directed to vp6 (42,000-dalton protein), the other major component of the inner capsid. these results indicated the possible presence of a subgroup-specific antigen on vp2 in ... | 1986 | 3002300 |
| rotavirus in nasopharyngeal secretions of children with upper respiratory tract infections. | nasopharyngeal secretions from 30 infants and children presenting with respiratory tract infection, were tested for rotavirus antigen. two of 30 children with signs and symptoms of seromucoid nasal secretions, cough, and low grade fever were positive for the antigen. nasopharyngeal secretions may play a role in the spread of this infection. | 1986 | 3002710 |
| overview of viral agents in pediatric enteric infections. | 1986 | 3003718 | |
| prevalence of various enteropathogens in the feces of diarrheic and healthy calves. | the presence of various enteropathogens was examined in the feces of homebred dairy calves reared in a restricted geographical area of france (north west of county of indre-et-loire) during winter 1983-1984. two distinct surveys were carried on: a case-control study including 32 diarrheic calves and 21 healthy calves bred in 53 different farms; and a separate study on nine diarrheic calves in another farm. the following infectious agents were looked for, by specific methods of detection: escheri ... | 1986 | 2877609 |
| protective effect of naturally acquired homotypic and heterotypic rotavirus antibodies. | to assess serotype specificity of immune resistance to rotavirus gastroenteritis, the relation between pre-existing neutralising antibodies to homotypic and heterotypic rotaviruses and protection against infection or clinical illness was investigated. the subjects were 44 orphans exposed once or twice to consecutive outbreaks of gastroenteritis due to type 3 rotavirus in an orphanage in sapporo. sera were collected throughout these outbreaks and the serum levels of neutralising antibodies agains ... | 1986 | 2874413 |
| rotavirus-associated diarrhea in a commercial rabbitry. | an epizootic of diarrheal disease occurred in a commercial specific-pathogen-free rabbitry, and was characterized by sudden onset, rapid spread, and high morbidity and mortality among sucklings. affected rabbits rapidly became dehydrated and most died within two days of the onset of diarrhea. eight of these rabbits were necropsied. five had blunted and fused small intestinal villi with attenuated villous enterocytes. a rotavirus was isolated from four rabbits, and five survivors of affected litt ... | 1986 | 2939288 |
| self-contained enzymic membrane immunoassay for detection of rotavirus antigen in clinical samples. | a self-contained enzymic membrane immunoassay (scemia) system has been developed for the detection of viral antigens in clinical samples. the assay system makes use of antiviral antibodies bound to a nylon membrane, a flow-through washing procedure, and a clearly visible endpoint of the enzymic reaction. a scemia system with antibodies against rotavirus detected rotavirus antigen, within 15 min, in all faecal samples from children with gastroenteritis that were positive for antigen in a standard ... | 1986 | 2878176 |
| the detection of enteropathogens in acute diarrhea in a family cohort population in rural egypt. | in 8 villages of rural northeastern egypt, a 2-year study of the etiologic agents associated with episodes of diarrhea was carried out. stool specimens (3,243) from 3,513 episodes of diarrhea were processed for enteropathogens. the most commonly identified agents in the group with diarrhea were giardia lamblia (44%), heat stable enterotoxin (st)-producing enterotoxigenic escherichia coli (etec) (15%), heat labile toxin (lt)-producing etec (12%), enteropathogenic e. coli (epec) (4%), rotavirus (3 ... | 1986 | 2876656 |
| prevalence of k88, k99, and 987p pili of escherichia coli in neonatal pigs with enteric colibacillosis. | one hundred nineteen live neonatal pigs with diarrhea less than or equal to 2 weeks old were euthanatized, and frozen sections of their ilea were submitted to an indirect fluorescent antibody technique to identify k88, k99, and 987p pili (also referred to as f-4, f-5, and f-6 pili, respectively) in escherichia coli. ten-centimeter ileal sections were used to determine numbers of lactose-fermenting bacteria. of 52 pigs in which e coli pili were found, 14 had k88 (27%), 23 had k99 (44%), 13 had 98 ... | 1986 | 2878634 |
| cultivation and characterization of three strains of murine rotavirus. | three distinct strains of murine rotavirus were adapted to growth in cell culture. these strains are genetically related but not identical; they are serotypically heterogeneous. the cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses were. homologous murine rotavirus strains spread from inoculated to uninoculated litter mates and caused diarrhea, while heterologous rotaviruses did not spread and cause illness. | 1986 | 3003390 |
| rotavirus-like infection associated with diarrhea in okapi. | 1986 | 2851576 | |
| failure of live, attenuated oral rotavirus vaccine. | 1986 | 2873370 | |
| [incidence of rotavirus in the menino jesus pediatric hospital (sp): elisa--a practical and efficient method for its detection]. | seventy infants and children with age from 2 months to 4 years old were admitted to "hospital infantil menino jesus", in são paulo, brazil, in the period from march 1983 to june 1984 with acute diarrhea. feces from these patients were analysed in the search of rotavirus and enteropathogenic bacteria (shigella, salmonella and e. coli), for the purpose of defining the role of rotavirus as an agent of acute diarrhea in infants and young children in this country. elisa (enzyme-linked immuno sorbent ... | 1986 | 2831862 |
| [ultrastructure of rotavirus]. | the human rotavirus is an icosadeltahedral structure, with a characteristic pattern of a triangulation number (t) of 13 with a skew symmetry. the inner layer of the rotavirus has 132 morphological units, with 260 structural units of trimetric structure. it is possible that the outer layer is a smooth geodesic shell. | 1986 | 2823323 |
| seroepidemiology of rotavirus in singapore. | 1986 | 2822792 | |
| derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. | 1986 | 2827408 | |
| genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. | two reassortant viruses were selected from a mixed infection of ma104 cells with human rotavirus strains wa (serotype 1-subgroup ii) and hn126 (serotype 2-subgroup i). antigenic characterization and genotype analysis by polyacrylamide gel electrophoresis revealed that they were reassortants with novel antigenic compositions, i.e. serotype 1-subgroup i (c116) and serotype 2-subgroup ii (c15). furthermore one of them, c15, was considered to have a mosaic antigenicity defined by two serotype-specif ... | 1986 | 2426394 |
| passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. | monoclonal antibodies directed against two rotavirus surface proteins (vp3 and vp7) as well as a rotavirus inner capsid protein (vp6) were tested for their ability to protect suckling mice against virulent rotavirus challenge. monoclonal antibodies to two distinct epitopes of vp7 of simian rotavirus strain rrv neutralized rrv in vitro and passively protected suckling mice against rrv challenge. a monoclonal antibody directed against vp3 of porcine rotavirus strain osu neutralized three distinct ... | 1986 | 2422398 |
| [immunotherapy in virus diseases]. | the number of effective anti-viral agents is still limited. therefore immunotherapy remains of importance in the treatment of viral infections. immunoglobulins and interferons as well as a variety of immune stimulators or immune modulators are available. the efficacy of such therapy strongly depends on the kind of the virus causing the infection as well as the time of initiation of the treatment. the prophylactic and therapeutic effects of immunoglobulin preparations are well documented, whereas ... | 1986 | 2422548 |
| rna-binding proteins of bovine rotavirus. | two major bovine rotavirus proteins have rna-binding activity as shown by an rna overlay-protein blot assay. of the six proteins in purified virions, only one showed rna-binding activity. this 92,000-molecular-weight (92k) protein was present in both single- and double-shelled particles. its rna-binding activity was blocked by preincubation with monospecific antibody to vp2. thus, the 92k rna-binding protein in rotavirus virions is vp2, the second most abundant protein in single-shelled particle ... | 1986 | 2422396 |
| protective effect of orally administered immunoglobulins against experimental calf diarrhea. | 1986 | 2423734 | |
| location of the major antigenic sites involved in rotavirus serotype-specific neutralization. | antigenic sites on the rotavirus major outershell glycoprotein were identified by using mutant viruses selected for resistance to neutralizing (serotype-specific) monoclonal antibodies. the glycoprotein genes from these mutants were sequenced to determine the position and nature of the resultant amino acid substitutions in the protein. three regions (a, b, and c) were identified (amino acids 87-96, 145-150, and 211-223, respectively), of which region c appears to be the most important. a mutatio ... | 1986 | 2422651 |
| derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. | neutralizing monoclonal antibodies were derived to human rotaviruses rv-4 (serotype 1), rv-5 (serotype 2), and st-3 (serotype 4). by enzyme immunoassay and fluorescent focus neutralization, eight of the antibodies appeared to be specific for the immunizing serotype, and so have potential as reagents for rotavirus serotyping by enzyme immunoassay. seven of these were shown by western blotting, enzyme immunoassay for antibody additivity, and reaction with rotavirus reassortants, to be directed aga ... | 1986 | 2429439 |
| prevention of calf diarrhea with an immunoglobulin diet in beef herds. | 1986 | 2431184 | |
| antigenic mapping of the surface proteins of rhesus rotavirus. | monoclonal antibodies have been produced and used to map the functional topography of the surface proteins of rhesus rotavirus (rrv) that mediate viral neutralization. ten monoclonal antibodies directed to vp7 were studied in neutralization assays and competitive binding studies. a large neutralization domain with several interrelated epitopes on vp7 was apparent. twelve monoclonal antibodies directed to vp3 were used in similar studies and delineated at least 2 distinct neutralization domains o ... | 1986 | 2431540 |
| synthesis of simian rotavirus sa11 double-stranded rna in a cell-free system. | a cell-free system was developed to study the replication of simian rotavirus sa11. the components of the system included (i) subviral particles prepared from infected cells to template the synthesis of viral rna and (ii) an mrna-dependent rabbit reticulocyte lysate to support protein synthesis. based upon nuclease-sensitivity, approximately 20% of the rna made in vitro was double-stranded (dsrna) and 80% single-stranded (ssrna). electrophoretic analysis of the rna products on polyacrylamide and ... | 1986 | 2437720 |
| a comparison of simian rotavirus sa11 preparations maintained in different laboratories. | preparations of simian rotavirus sa11 maintained in different laboratories were compared with each other by polyacrylamide gel electrophoresis of genomic rna. differences in the migration of genome segments 4, 5 and 7 allowed the classification of eight virus preparations into four electrophoretic types. | 1986 | 3039290 |
| [immunoenzyme analysis in the diagnosis of human rotavirus gastroenteritis (methodological aspects)]. | 1986 | 3030005 | |
| comparison of reverse passive haemagglutination assay & solid phase agglutination of coated erythrocytes with elisa for rotavirus antigen detection. | 1986 | 3028947 | |
| [rotavirus reinfections in children of belém, pará, brazil]. | 1986 | 3029854 | |
| [rotaviruses: their structure, chemical composition and biological properties]. | 1986 | 3029998 | |
| incidence of rotavirus infection in pediatric outpatients with gastroenteritis. | 1986 | 3030974 | |
| [technics used in viral genetics as an alternative means of diagnosing viral diseases]. | 1986 | 3031336 | |
| epidemiology of human rotaviruses in argentina as determined by rna genome electrophoresis. | rotavirus gastroenteritis was studied by enzyme-linked immunosorbent assay (elisa) and by page from april 1983 to april 1985 at the buenos aires ricardo gutierrez children's hospital and at the san justo children's hospital. based on examination of 576 cases, rotavirus was identified in 109 (18.9%) cases by elisa and in 99 (17.2%) cases by page. as a diagnostic tool page presented a sensitivity of 90.8% compared with elisa. compared with the control sa-11 genome, 84 samples (84.9%) presented a l ... | 1986 | 3034823 |
| [comparative study of the effectiveness of immunoelectron microscopic methods for detecting rotaviruses and the hepatitis a virus]. | different variants of immune electron microscopy method used for the detection of rotaviruses and hepatitis a virus in specimens from patients were compared. immune electron microscopy using filtration into agar was shown to be the optimal method for diagnosis of such prevalent infections as rotavirus gastroenteritis and viral hepatitis a. | 1986 | 3026094 |
| [use of the agar diffusion precipitation method for detecting rotavirus antigen]. | the possibility of rotavirus detection in feces of patients with acute enteric diseases by the agar gel diffusion (agd) test was studied. the effectiveness of this method was compared with that of the standard method, direct electron microscopy. both methods showed good correlation of the results, but the agd test is methodically much simpler which recommends it for diagnosis of rotavirus infection. rotavirus-specific hyperimmune calf serum may by used as a serological diagnostic preparation for ... | 1986 | 3026098 |
| combined vaccination with live oral polio vaccine and the bovine rotavirus rit 4237 strain. | a clinical trial was carried out in 3-month-old infants to assess whether concomitant oral administration of live polio and rotavirus rit 4237 vaccines would reduce their immunogenicities as a result of mutual interference. one hundred and sixty breast-fed male and female infants were randomly allocated to four study groups to receive in a blind fashion the poliovirus vaccine, the rit 4237 vaccine, a combination of both vaccines or a placebo preparation. antibody titres were measured in pre- and ... | 1986 | 3026106 |