Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| refined crystal structure of calcium-liganded carp parvalbumin 4.25 at 1.5-a resolution. | the crystal structure of carp parvalbumin (pi = 4.25) has been refined by restrained least-squares analysis employing x-ray diffractometer data to 1.5-a resolution. the final residual for 12,653 reflections between 10 and 1.5 a with i(hkl) greater than 2 sigma(i) is 0.215. a total of 74 solvent molecules were included in the least-squares analysis. the root mean square deviation from ideality of bond lengths is 0.024 a. the model has a root mean square difference of 0.59 a from the positions of ... | 1990 | 2334704 |
| glycogen phosphorylase in fish muscle: demonstration of three interconvertible forms. | white skeletal muscle of crucian carp contains a single isoenzyme of glycogen phosphorylase, which was purified approximately 300-fold to a specific activity of approximately 13 mumol.min-1.mg protein-1 (assayed in the direction of glycogen breakdown at 25 degrees c). tissue extracts of crucian muscle produced three distinct peaks of phosphorylase activity when separated on deae-sephacel. peaks 1 and 3 were identified, in terms of kinetic properties and by interconversion experiments, as phospho ... | 1990 | 2106266 |
| sequential course of uptake of intravitreal 5,7-dihydroxytryptamine by carp retinal cells. | the sequential course of uptake by retinal cells of intravitreally injected 5,7-dihydroxytryptamine (5,7-dht) together with dopamine (da) was investigated in juvenile carp retinas, which were removed at various intervals (1-24 h) after injection. the cells taken up 5,7-dht were visualized immunohistochemically with anti-serotonin (5-ht) antibody and fitc-conjugated igg. after a mixture of 5,7-dht and da (2.5, 10 or 20 micrograms each) was given, large-sized indoleamine (ia) amacrine cells first ... | 1990 | 1970941 |
| functional coupling of glycolysis and phosphocreatine utilization in anoxic fish muscle. an in vivo 31p nmr study. | three fish species with different strategies for anoxic survival (goldfish, tilapia, and common carp) were exposed to environmental anoxia (4, 3, and 1 h, respectively). the concentrations of high energy phosphate compounds and inorganic phosphate, besides the intracellular ph in the epaxial muscle were measured during anoxia and recovery by in vivo 31p nmr spectroscopy. the concentration of free adp was calculated from the equilibrium constant of creatine kinase. during anoxia the patterns of p ... | 1990 | 2295625 |
| watercress and amphipods potential chemical defense in a spring stream macrophyte. | we investigated the potential role of defensive chemicals in the avoidance of watercress (nasturtium officinale) by the cooccurring amphipod,gammarus pseudolimnaeus at two spring brooks: carp creek, michigan and squabble brook, connecticut. we conducted observations and laboratory experiments on the consumption of watercress, the toxicity of damaged (frozen) watercress, and the toxicity of damage-released secondary chemicals. field-collected yellowed watercress typically lacked the bite and odor ... | 1990 | 24264910 |
| effects of paraquat treatment on fish transaminase molecular subforms. | the effects of paraquat (pq; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. the serum glutamic-oxalacetic transaminase (got; l-aspartate: 2-oxoglutarate aminotransferase, ec 2.6.1.1.) level was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in all species, and there was a change in the distribution of the molecular subforms of got in the liver and heart. the activities of the individual subforms decreased with increasing p ... | 1990 | 24221900 |
| bacterial flora of fishes: a review. | bacterial floras isolated from eggs, skin, gills, and intestines have been described for a limited number of fish species. generally, the range of bacterial genera isolated is related to the aquatic habitat of the fish and varies with factors such as the salinity of the habitat and the bacterial load in the water. in many investigations, identification of isolates to the genus level only makes it difficult to determine the precise relationships of aquatic and fish microfloras. bacteria recovered ... | 1990 | 24196252 |
| electroretinographic b-wave merely reflects the activity of the rod system in the dark-adapted carp retina. | the electroretinogram and responses of intermediate horizontal cells (ihcs) were recorded simultaneously and studied under dark-adapted conditions in intact crucian carp, with the hope of providing an answer to the controversy whether the scotopic b-wave contains contribution from both rods and cones. our observations indicate that the dark-adapted b-wave follows the univariance principle perfectly in that: (1) b-wave forms and shapes of response amplitude vs light intensity relationships did no ... | 1990 | 2392843 |
| interaction between the soma and the axon terminal of horizontal cells in carp retina. | in teleost retina, the receptive fields of horizontal cell axon terminals have a larger space constant than the receptive fields of the horizontal cell somata. generally this difference in receptive field size is attributed to the cell coupling which is assumed to be stronger in the horizontal axon terminal network than in the horizontal cell soma network. the axon terminals are displaced with respect to the cell bodies. in this paper we show, using a simple simulated horizontal cell (hc) networ ... | 1990 | 2392830 |
| a cell line (clc) of adherent peripheral blood mononuclear leucocytes of normal common carp cyprinus carpio. | 1990 | 2369971 | |
| comparative anatomy of the intestine of cyprinus carpio linnaeus 1758 and salmo trutta linnaeus 1758. | the anatomy of carp and trout was compared by means of anatomical dissection and frozen cross-sections. this enabled exact topographical localization and hence also the determination of differences in the position of individual intestinal segments. the elaboration of a suitable nomenclature was considered to be an important working measure for the facilitation of interspecific comparison. | 1990 | 2341080 |
| surface location and stage-specificity of differentiation antigens on germ cells in the common carp (cyprinus carpio), as revealed with monoclonal antibodies and immunogold staining. | during development of juvenile and young adult carp (cyprinus carpio, l., teleostei) three differentiation stages were distinguished in the testis: the prespermatogenic, the early spermatogenic and the advanced spermatogenic testis. carp testis tissue of these stages was dissociated by enzymatic digestion and viable testis cells with well preserved morphological features were obtained. the surface location and stage-specificity of differentiation antigens on these germ cells was investigated usi ... | 1990 | 2286535 |
| compound action potential amplitude and conduction velocity as a function of temperature in carp nervus lateralis. | this study shows the relationship between nerve fiber conduction velocity of compound action potentials produced by bipolar electrical stimulation were recorded from the isolated carp nervus lateralis by means of an electrode array consisting of 16 0.5 mm diameter platinum wire electrodes mounted in a nerve chamber. the recordings were repeated at temperatures ranging from 12.0 to 32.5 degrees c. both the amplitude and conduction velocity of the nerve response peaked at about 28.0 degrees c. fir ... | 1990 | 2271406 |
| structure of the toxin isolated from carp (cyprinus carpio) bile. | attempts were made to elucidate the structure of the toxin isolated from the bile of carp cyprinus carpio, which is possibly responsible for carp poisoning. by fast atom bombardment (fab) mass spectrometry, along with 1h- and 13c-nmr, a molecular formula of c27h48o8s containing a sulfate ester group was deduced. those and other analytical data allowed us to conclude the structure of carp toxin to be 5 alpha-cholestane-3 alpha, 7 alpha, 12 alpha, 26, 27-pentol 26-sulfate, which agreed essentially ... | 1990 | 2260104 |
| a presumptive role for gaba in the stimulatory effects of des-gly10, [d-ala6]-lhrh-ethylamide and pimozide on the gonadotropin release in carp. | to investigate the effect of endogenous gamma-aminobutyric acid (gaba) on the blood maturating gonadotropin (gth) levels, or to study its interaction with pimozide (dopamine antagonist) and a luteinizing hormone-releasing hormone analog (lhrh-a), sexually mature male and female carps were treated with drugs that may either inhibit gaba biosynthesis or gaba degradation. in females the irreversible inhibitor of gaba-transaminase, gamma-vinyl gaba (gvg), which was to increase the endogenous gaba-er ... | 1990 | 2259268 |
| influence of dietary magnesium on mineral, ascorbic acid and glutathione concentrations in tissues of a freshwater fish, the common carp. | supplementation with dietary mg at a minimum level of 0.06% seems to be essential to prevent the hypercalcinosis of the kidney and hepatopancreas in a fish, the common carp. mg deficiency appears to have no effect on the mg level in kidneys and hepatic tissue, whereas the fe level in those tissues was significantly diminished by increasing dietary mg supplementation up to 3.2 g.kg-1. both hypercalcinosis and accumulation of fe in soft tissues were more pronounced in fish offered diets high in pr ... | 1990 | 2222799 |
| neurotoxic effects of l-alpha-aminoadipic acid on the carp retina: a long term observation. | the hypothesis has been tested that the enantiomers of alpha-aminoadipic acid have different target effects; the l-isomer has both glio- and neurotoxic actions, while the dl-isomer has a gliospecific action in the cns. electrophysiological and morphological studies were carried out on the retina of the carp (cyprinus carpio) for one to two months after intraocular injection with alpha-aminoadipic acids at various doses. intracellular recording from horizontal cells and extracellular recording of ... | 1990 | 2215916 |
| cyclical changes in the pituitary gonadotrophs (gth cells) in relation to the testicular cycle in puntius sarana (hamilton). | the pituitary gland of puntius sarana is of the leptobasic type and has three subdivisions--a proximal pars distalis (ppd), a rostral pars distalis (rpd) and a pars intermedia (pi) arranged vertically one below the other. the gonadotrophs (gth cells) of the ppd were identified by their pas, ath, af, aniline blue and alcian blue positivity and by their immunoreactivity to ovine anti-lh beta-subunit, anti-salmon gth and anti-silver carp gth. the testes of puntius sarana have been classified as the ... | 1990 | 2210495 |
| calcium ions as a mediator in gnrh action on gonadotropin release in the common carp (cyprinus carpio l). | collagenase-dispersed carp pituitary cells in a perifusion system were used to study the role of calcium ions in the mechanism of gnrh action on the release of maturational gonadotropin (gth) in fish. the specific calcium chelator egta and the calcium antagonist manganese (mn2+) caused a 40% inhibition in the basal gth release and completely blocked gnrh-stimulated gth release. short-term application of graded doses of calcium ionophore a23187 caused a dose-dependent increase in gth secretion. a ... | 1990 | 2123110 |
| localization of growth hormone-releasing factor-like immunoreactivity in the hypothalamo-hypophysial system of some teleost species. | an antiserum to growth hormone-releasing factor (grf) 1-44 was applied on brain and pituitary sections of nine teleost species. immunoreactive (ir) perikarya were demonstrated in parvo- and magnocellular portions of the preoptic nucleus (pon) and occasionally in the nucleus lateralis tuberis. the two tracts originating in the pon ran ventro-laterally toward the optic chiasm and then caudally in the basal hypothalamus. in the pars distalis (pd) of the eel, carp, goldfish and salmonids, grf-ir fib ... | 1990 | 2105165 |
| absorption of ascorbic acid and ascorbic sulfate and ascorbate metabolism in common carp (cyprinus carpio l.). | ascorbate metabolism was analyzed in fasted common carp and carp offered diets lacking ascorbic acid or supplemented with ascorbic acid (aa) or ascorbic sulfate (as). ascorbic acid and ascorbic sulfate were analyzed in the contents collected from various parts of the digestive tract. the major site of the dietary ascorbate absorption was located in the first 20% of the anterior intestine region (58.7 +/- 10.2%), whereas absorption increased to 94.3 +/- 1.9% (in the whole gut). considerable secre ... | 1990 | 2097301 |
| cytotoxicity of carp (cyprinus carpio) leucocytes induced against tnp-modified autologous spleen cells and influence of acclimatization temperature. | 1990 | 2086276 | |
| dopamine and 2-amino-4-phosphonobutyrate differentially modify spectral responses of h1 horizontal cells in carp retina. | cone-driven external h1 horizontal cells (h1 hcs) in the cyprinid fish retina hyperpolarize in response to all visible lights, and their synaptic inputs have been widely believed to be excitatory. recent experiments indicate, however, that short- and long-lambda (wavelength)-sensitive cone photoreceptors have different types of synaptic mechanisms; a conductance-decreasing, sign-reversing and short-lambda-mediating type, and a more conventional conductance-increasing class of excitatory (sign-co ... | 1990 | 2073952 |
| effects of some autonomic drugs and neuropeptides on the mechanical activity of longitudinal and circular muscle strips isolated from the carp intestinal bulb (cyprinus carpio). | 1. the mechanical responses to some autonomic drugs and neuropeptides of longitudinal muscle (lm) and circular muscle (cm) strips isolated from the carp intestinal bulb were investigated in vitro. 2. acetylcholine and carbamylcholine caused concentration-dependent transient contraction of both lm and cm strips. tetrodotoxin had no effect, but atropine selectively decreased the contractile responses to acetylcholine and carbamylcholine. 3. excitatory alpha-2 and inhibitory beta adrenoceptors were ... | 1990 | 1981339 |
| seasonal variations in the content of catecholamines in carp heart (cyprinus carpio). | 1. seasonal changes in epinephrine and norepinephrine contents were demonstrated in ventricular muscle tissues of carp heart. 2. patterns of changes in epinephrine and norepinephrine contents were similar; high during late spring-to-summer and low in winter; however, the fluctuation in epinephrine content was greater than that of norepinephrine. 3. tyramine caused a positive inotropic effect in isolated electrically-stimulated ventricular muscles of carp heart. the inotropic effect was totally b ... | 1990 | 1981337 |
| bioconcentration and excretion of diazinon, ibp, malathion and fenitrothion by carp. | 1. bioconcentration and excretion of diazinon, ibp, malathion and fenitrothion were studied for carp (cyprinus carpio l.). 2. the concentrations of these pesticides in muscle and viscera of the carp reached plateaus in 12-48 hr exposure. 3. the average values of bioconcentration factors (bcf) for diazinon were 20.9 in muscle, 60.0 in liver, 111.1 in kidney and 32.2 in gallbladder over the 168 hr exposure period. similarly, those values were 4.3-26.7 for ibp, 2.7-17.3 for malathion, and 36.0-157. ... | 1990 | 1980880 |
| accumulation and metabolism of 2,4,6-trichlorophenyl-4'-aminophenyl ether by carp. | 1. accumulation of 2,4,6-trichlorophenyl-4'-aminophenyl ether (cnp-amino) in carp was investigated for 14 days. cnp-amino in carp could be divided into free cnp-amino [cnp-amino(i)] and bound cnp-amino [cnp-amino(ii)]. 2. the bioconcentration factors (bcf) of cnp-amino(i + ii) in carp were 90 +/- 38 (mean +/- sd, n = 3) for muscle, 402 for liver, 501 for kidney and 5368 for gallbladder after 14 days exposure. 3. 2,4,6-trichlorophenyl-4'-acetamide phenyl ether (cnp-acetamide) was detected as meta ... | 1990 | 1980874 |
| gliotoxic effects of alpha-aminoadipic acid isomers on the carp retina: a long term observation. | the glutamate analogue, alpha-aminoadipic acid was intravitreally administered in the d-, dl- and l-forms to carp (cyprinus carpio) retina in vivo. to make a quantitative assessment of its gliotoxic action, the activity of glutamine synthetase, whose localization was confirmed in glial müller cells by an immunohistochemical technique, was examined at various intervals over one month. intravitreal injection of 8 mumol alpha-aminoadipic acids reduced the glutamine synthetase activity within 4 h an ... | 1990 | 1977098 |
| involvement of voltage-dependent calcium channels (vdcc) in the action of gnrh on gth release in common carp (cyprinus carpio l): comparison with k+ action. | the involvement of different types of voltage-dependent calcium channels (vdcc) in the stimulatory action of gnrh (in comparison with k+) on maturational gonadotropin (gth) release was investigated using superfused carp pituitary cells. the action of these 2 stimulants was not modified either by d600 or nifedipine (drugs blocking l-type of vdcc). cadmium (cd2+), which blocks all types of vdcc indifferently, provoked a dose-dependent stimulation of gth secretion. cd2+ action was not altered by ad ... | 1990 | 1705429 |
| ca2(+)-binding site of carp parvalbumin recognized by monoclonal antibody. | monoclonal antibody 235 which was used for immunohistochemical staining of parvalbumin in tissue sections partially protects lys-54 of carp muscle parvalbumin from reaction with acetic anhydride in the parvalbumin-antibody complex. lys-54 is located in the cd-loop of parvalbumin and is flanked by the ca2(+)-ligands asp-53 and ser-55 of the ca2(+)-site i. another monoclonal antibody against carp parvalbumin, mca 239, partially protects lysine residues 27, 32, 87 and 107, indicating that this anti ... | 1990 | 1690078 |
| preparation of antiserum against carp (cyprinus carpio l.) immunoglobulin and its application for immunohistochemistry. | 1990 | 2287143 | |
| effect of species differences and dietary vitamin c on the concentration of ascorbate- and acid-soluble thiol in fish eye. | data presented confirm the essentiality of modification of the dinitrophenylhydrazine (dnph) method to analyze the total ascorbic acid and dehydroascorbic acid in ocular tissues and stress the need of corrections for the interfering substances. variations in ascorbate and thiol concentrations in the lens, retina and aqueous humour of freshwater fish belonging to the cyprinidae family were examined. the interspecific variability of ascorbate concentration was highest in the aqueous humour and low ... | 1990 | 2265674 |
| [prophylactic and therapeutic studies on intestinal giant-cystic disease of the israel carp caused by thelohanellus kitauei. ii. effects of physical and chemical factors on t. kitauei spores in vitro]. | in a basic attempt to develop the prophylactic and therapeutic measures on intestinal giant-cystic disease of the israel carp, cyprinus carpio nudus, the effects of physical and chemical factors on viability or survival of the spores of thelohanellus kitauei were checked in vitro by means of extrusion test on the polar filament. when the fresh spores suspended with 0.45% and 0.9% sodium chloride solution and distilled water were laid at 5 degrees c and 28 degrees c for short terms, the extrusion ... | 1990 | 2099178 |
| modulatory effect of metal ions on the immune response of fish: in vivo and in vitro influence of mncl2 on nk activity of carp pronephros cells. | the in vivo and in vitro influence of mncl2 on carp pronephros cells was investigated. increased cytotoxicity against both yac-1 and p 815 target cells was observed following an intraperitoneal injection of 40, 80, or 120 micrograms mncl2/g body wt administrated 24 hr prior to the in vitro 51cr release assay. similarly, in vitro treatment of carp pronephros cells, at a final concentration of 60 micrograms/culture, resulted in an increase of nk cell activity in both yac-1 and p 815 target cell li ... | 1990 | 2090440 |
| interrelationships between red cell nucleoside triphosphate content, and blood ph, o2-tension and haemoglobin concentration in the carp, cyprinus carpio. | as a starting point for investigations of possible control factors for the nucleoside triphosphate content in carp red cells, we utilized the natural variability in blood physico-chemical parameters to test for interrelationships. by application of two-variable regression analysis, the red cell ntp content was found to be significantly correlated with arterial po 2 and ph as well as with the blood haemoglobin (hb) concentration. these correlations show a rise in red cell ntp content with falling ... | 1990 | 24221032 |
| the influence of temperature on muscle velocity and sustained performance in swimming carp. | the aim of this study was to evaluate how fish locomote at different muscle temperatures. sarcomere length excursion and muscle shortening velocity, v, were determined from high-speed motion pictures of carp, cyprinus carpio (11-14 cm), swimming steadily at various sustained speeds at 10, 15 and 20 degrees c. in the middle and posterior regions of the carp, sarcomeres of the lateral red muscle underwent cyclical excursions of 0.31 microns, centered around the resting length of 2.06 microns (i.e. ... | 1990 | 2277258 |
| effect of sublethal doses of three pesticides on the ovary of a carp minnow rasbora daniconius. | 1990 | 2265274 | |
| [hormonal stimulation, in vivo, of the ovary of european eel in the yellow stage]. | the immature ovary of yellow eel was stimulated by a long-term treatment with a gonadotropin ii rich extract of carp pituitary. ovarian follicles of treated yellow eels showed an accumulation of lipidic vacuoles in the oocyte cytoplasm and a thickened follicular envelope (in this regard, they resembled the ovarian follicles of previously studied silver eels). the treatment also resulted in a significant but limited increase in the gonadosomatic ratio and mean follicle size (these values were muc ... | 1990 | 2291806 |
| cytoarchitecture and fiber connections of the nucleus lateralis valvulae in the carp (cyprinus carpio). | the cytoarchitecture and fiber connections of the nucleus lateralis valvulae of the carp (cyprinus carpio) were studied by nissl, bodian, golgi, and horseradish peroxidase methods. chief cells composing the nucleus lateralis valvulae (nlv) are small and granular, and their axons terminate in the cerebellum. these neurons have no dendrite, and the cell body is enveloped in a single terminal of afferent fibers to the nlv. in order to observed local cell clustering, the nlv was three-dimensionally ... | 1990 | 2229471 |
| gaba-ergic and glycinergic pathways in the inner plexiform layer of the goldfish retina. | gaba-ergic and glycinergic circuitry in the inner plexiform layer of the goldfish retina was evaluated by electron microscopic autoradiography of 3h-gaba and 3h-glycine uptake, combined with retrograde horseradish peroxidase (hrp) labeling of ganglion cells. gaba-ergic and glycinergic synapses were found on labeled ganglion cells throughout the inner plexiform layer. this reinforces the idea that physiological evidence of gaba-ergic and glycinergic influence on a variety of ganglion cells in gol ... | 1990 | 2298935 |
| isolation, amino-acid sequence, synthesis and biological properties of urotensin i from hippoglossoides elassodon. | abstract a 41-residue urotensin i neuropeptide (h-ui) was isolated from urophyses of the marine teleost hippoglossoides elassodon (the flathead sole). the peptide was recognized by its partial cross-reactivity in a radioimmunoassay developed for catostomus (sucker) ul (s-ui), and was purified by reversed-phase high-performance liquid chromatography. the amino-acid sequence was shown to be h-ser-glu-glu-pro-pro-met-ser-lle-asp-leu-thr-phe-his-met-leu-arg-asn-met-lle-his-arg-ala-lys-met-glu-gly-gl ... | 1990 | 19215432 |
| the development of a radioimmunoassay for carp, cyprinus carpio, vitellogenin. | the development of an easily - performed and robust radioimmunoassay (ria) to carp, cyprinus carpio, vitellogenin (c-vtg) is described. purified c-vtg was iodinated using iodogen. the resulting c-vtg label was useful for up to 60 days. high titre antibodies were raised in rabbits to the purified c-vtg. the practical operating range of the c-vtg ria was between 2 and 200 ng. ml(-1). vtg was detected in plasma from all female mirror carp investigated, and all plasmas diluted parallel to the standa ... | 1990 | 24221946 |
| brain growth patterns in four european cyprinid fish species (cyprinidae, teleostei): roach (rutilus rutilus), bream (abramis brama), common carp (cyprinus carpio) and sabre carp (pelecus cultratus). | this study compares brain growth in 4 species of cyprinids, each distinctly different in adult brain morphology: roach have generalized brains; bream are characterized by well-developed visual, octavolateralis and gustatory brain regions; common carp show chemosensory (gustatory)-dominated brains, and sabre carp octavolateralis-dominated brains. the growth patterns of 16 regions relative to total brain volume were investigated by computer-aided quantitative histology to illustrate internal brain ... | 1990 | 2379081 |
| influence of quaternary structure of the globin on thermal spin equilibria in different methemoglobin derivatives. | we have measured the paramagnetic susceptibilities of sperm whale azide metmyoglobin and of carp azide, thiocyanate, and nitrite methemoglobin in the quaternary oxy (r) and deoxy (t) structures between about 300 and 90 k, using a new sensitive superconducting magnetometer. we have also measured the pressure dependence of the high- and low-spin optical absorption bands of azide metmyoglobin and of carp azide methemoglobin in the r and t structures between 1 and 2000-4000 atmospheres. at low tempe ... | 1990 | 210801 |
| substrate specificity for myelin basic protein-specific protein methylase i. | the substrate specificity of bovine brain myelin basic protein (mbp)-specific protein methylase i (s-adenosyl-l-methionine:protein-l-arginine n-methyltransferase, ec 2.1.1.23), which methylates arginine residues of protein, has been studied using various mbps, several synthetic peptides and heterogeneous nuclear ribonucleoprotein complex protein (hnrnp). (1) among mbps from different species of brain, the carp mbp was found to be the best substrate for mbp-specific protein methylase i. this high ... | 1990 | 1694691 |
| growth hormone-dependent potentiation of gonadotropin-stimulated steroid production by ovarian follicles of the goldfish. | the possible involvement of growth hormone (gh) in the regulation of ovarian function in the goldfish was investigated by determining the effects of common carp gh on steroid production by vitellogenic and preovulatory ovarian follicles incubated in vitro. carp gh acts in a dose-dependent manner to potentiate the actions of common carp gonadotropin (gth) on the production of 17 beta-estradiol and testosterone by vitellogenic ovarian follicles and the actions of human chorionic gonadotropin (hcg) ... | 1990 | 1697273 |
| development and validation of a competitive enzyme immunoassay for chum salmon prolactin: a comparison to radioimmunoassay. | an enzyme immunoassay (eia), based on a competitive assay system, for the measurement of prolactin (prl) in the pituitary of salmonid fishes and of hormone released in medium from incubated pituitary was developed using a rabbit antiserum to chum salmon prl (prl, a combination of prl i and prl ii). chum salmon prl was coupled to horseradish peroxidase (hrp). the incubation procedure for the antigen-antibody reaction was analogous to that in the radioimmunoassay (ria) for prl. the antibody-bound ... | 1990 | 1705233 |
| presence of four tachykinins in an acid extract of the carp intestinal bulb (cyprinus carpio). | 1. the pharmacological and chemical properties of substance p-like peptides isolated from an acid extract of the carp intestinal bulb were examined using guinea-pig ileum longitudinal smooth muscle. 2. on a sephadex g25 column (3 x 96 cm), smooth muscle contracting material was eluted as two peaks (fraction-1 and fraction-2). the molecular weight of the fraction-1 was estimated to be 2300 and that of the fraction-2 to be 1530. 3. the pharmacological properties of the contracting materials in fra ... | 1990 | 1705490 |
| distribution and migration pathways of hnk-1-immunoreactive neural crest cells in teleost fish embryos. | whole mounts and cross-sections of embryos from three species of teleost fish were immunostained with the hnk-1 monoclonal antibody, which recognizes an epitope on migrating neural crest cells. a similar distribution and migration was found in all three species. the crest cells in the head express the hnk-1 epitope after they have segregated from the neural keel. the truncal neural crest cells begin to express the epitope while they still reside in the dorsal region of the neural keel; this has ... | 1990 | 1706978 |
| tropomyosin from the striated muscles of carp (cyprinus carpio) and of icefish (channichthys rhinoceratus). | tropomyosin of fast-twitch, slow-twitch and cardiac muscles of carp and icefish has been isolated by hydroxyapatite chromatography. the subunit distribution has been investigated by polyacrylamide gel electrophoresis and by peptide mapping. the purified skeletal muscle tropomyosins all belong to the alpha family and differ from higher vertebrate tropomyosin by the lack of beta subunits. specific alpha isotypes are however encountered in fast-twitch fibres (alpha w subunit) and slow-twitch or int ... | 1990 | 1708998 |
| [characteristics of adenosine triphosphatase activity in carp erythrocytes treated with saponin]. | the activities of ca-atpase and na,k-atpase in saponin-treated erythrocytes of man, rat and carp were compared. it was shown that at free calcium concentrations lower than 1 microm the activity of ca-atpase in carp erythrocytes was by one order of magnitude lower than in rat erythrocytes and 3-4 times lower than in human red blood cells. at [ca2+] = 0.4 microm the activities of na,k-atpase in all species under study were essentially the same. the increase in ca2+ concentration up to 1 microm res ... | 1990 | 1963091 |
| isoelectric analysis of 3h-pargyline-labelled monoamine oxidase in rat and carp. | 1. after selective binding of [3h]pargyline to either monoamine oxidase (mao) a or mao b in the rat liver, mao b alone in the rat brain and mao in carp brain and liver, molecular weight and isoelectric points (pi) of these mao were determined by sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis and isoelectric focusing and results obtained were compared. 2. for all tissues tested, sds-polyacrylamide gel electrophoresis of [3h]pargyline-bound samples revealed a labelled protein ban ... | 1990 | 1980888 |
| growth of organs and tissues in carp (cyprinus carpio l.) larvae. | growth of some internal organs and tissues was studied in carp (cyprinus carpio l.) in order to provide baseline information on the growth process during the first posthatching stages of fish, and to compare the trend observed in fish to that known in other animals. growth of organs and tissues (liver, pancreas, trunk muscles, digestive tract, eyes, nervous system) was studied in rapidly growing carp (cyprinus carpio l.) larvae by means of a quantitative histological method. mean body weight of ... | 1990 | 2074119 |
| conformational comparisons among homologous bony fish 5s ribosomal rnas. | the reversible thermal melting of homologous 5s ribosomal rnas of three bony fishes, rainbow trout (salmo gairdneri), carp (cyprinus carpio), and dog salmon (onchorhynchus keta), were compared in aqueous salts (sodium chloride and/or divalent metal chlorides) by means of differential optical absorption at 260 and 280 nm, and c.d. at 265 nm. the melting parameters of the multiphasic melting process were conserved among the homologous rnas, and it was shown that they did not depend on the methods ... | 1990 | 2083239 |
| [the seasonal appearance of swim bladder inflammation in the course of sphaerospora renicola infection in carp]. | in carp during 1st year the course of dynamics of the 3 known stages of sphaerospora renicola showed an increase of the kidney infection in winter and spring (with inhibited sporulation). the winter/spring-peak was accompanied by an intensive blood-phase (c-stages). after august of the 1st summer the swimbladder-phase (k-stages) was not observed, although sbi occurred between january and april. under warm water condition this infection was discharged after september/october, the sbi never occurr ... | 1990 | 2085211 |
| [a novel group of nucleolar organizer region associating proteins]. | with the scleroderma autoantinucleolar antiserum which was discribed in our previous paper, we identified a novel group of nucleolar organizer associating proteins, anop. by means of immunoblotting, it was found that anop mainly consists of three polypeptides with molecular weights of 65, 76, and 78 kd respectively. immunoelectron microscopy demonstrated that anop is localized in the shells of dense fibril components surrounding fibrillar centers of nucleolus. moreover, indirect immunofluorescen ... | 1990 | 2091420 |
| rna-binding protein-related sequence in a malaria antigen, clustered-asparagine-rich protein. | members of the rna-binding protein superfamily contain rna binding domains of about 90 amino acids with a highly conserved motif 'gfgf'. using the conserved motif with some variations g-(f/y)-(g/a)-(f/y)-(v/i)-x-(f/y) as a probe, we screened protein sequences carrying identical amino acids in an nbrf-protein database. it has been found that the c-terminal portion of clustered asparagine-rich protein (carp), a malaria antigen from plasmodium falciparum, shows an unexpected sequence similarity wit ... | 1990 | 2105236 |
| growth of the penaeid shrimp virus infectious hypodermal and hematopoietic necrosis virus in a fish cell line. | an established fish cell line, epithelioma papillosum cyprini (epc), originating from the carp (cyprinus carpio) was found to be susceptible to the penaeid shrimp virus, infectious hypodermal and hematopoietic necrosis virus (ihhnv). this economically important pathogen of penaeid shrimp raised in mariculture has been previously limited to growth only in young post-larval shrimps. this is, to our knowledge, the first reported successful growth of the penaeid virus in a fish cell line; it promise ... | 1990 | 2117021 |
| intestinal trematodes of humans in korea: metagonimus, heterophyids and echinostomes. | ten species of the trematode family heterophyidae (metagonimus yokogawai, m. takahashii, heterophyes nocens, h. heterophyes, h. dispar, heterophyopsis continua, pygidiopsis summa, stellantchasmus falcatus, centrocestus armatus and stictodora fuscatum), and 3 species of the family echinostomatidae (echinostoma hortense, e. cinetorchis and echinochasmus japonicus) have been found to infect humans in korea. biological and epidemiological studies on the above species have shown that metagonimus, het ... | 1990 | 2133416 |
| isolation and characterization of beta-actin gene of carp (cyprinus carpio). | a beta-actin gene of carp (cyprinus carpio) was isolated from a genomic embl3 library. the nucleotide sequence of the gene indicates six exons spanning 3.6 kb. southern blot hybridization of restriction endonuclease digests of carp genomic dna indicate that there are two copies of the beta-actin isotype and several other species of actin genes. the transcriptional start site is 85 bp and 24 bp downstream respectively from consensus ccaat and tata promoter elements. the organization of the carp b ... | 1990 | 2134183 |
| [the biology and pathogenicity of the carp parasite thelohanellus nikolskii (myxosporidia)]. | the myxosporidian thelohanellus nikolskii has been recorded in young of carp in fish-breeding ponds of moldavia since 1982. sporogenesis takes place from june to august. single cysts with mature spores are found in winter and spring. the distribution of cysts in the host's population is of overdispersed character. haematological investigation has revealed the threshold level of the invasion when there are observed changes in the blood of infected fishes (8 to 10 cysts per 1 g of fish weight). th ... | 1990 | 2147986 |
| purification and subcellular localization of zn-dependent acid p-nitrophenylphosphatase in frog liver and comparison with other vertebrates. | zn2(+)-dependent acid p-nitrophenylphosphatase (zn-acpase) from liver of rana esculenta was purified to homogeneity. the purified enzyme moves as a single electrophoretic band at ph 8.3 in 7.5% acrylamide and was coincident with the enzyme activity. the enzyme has a molecular weight of 102,000 +/- 5,000d and is a dimer with two apparently similar polypeptide chains of 48,000 +/- 3,000d as determined by sodium dodecylsulfate gel electrophoresis. zn-acpase from frog liver requires zn2+ ions for ca ... | 1990 | 2161444 |
| angiotensin-converting enzyme distribution and hypoxia response in mammal, bird, and fish. | angiotensin i-converting enzyme activity was measured in homogenates of guinea pig (cavia porcellus), chicken (gallus domesticus), and carp (cyprinus carpio) organs. the highest activity was found in guinea pig lung and chicken kidney. in carp the highest activity was found in heart and spleen, although gill arch also showed a high activity. acute hypoxia decreased the angiotensin i-converting enzyme activity in guinea pig lung and carp gill arch, but the changes in chicken lung and kidney were ... | 1990 | 2167864 |
| herpesviruses detected in papillomatous skin growths of koi carp (cyprinus carpio). | herpesviruses were found associated with epidermal hyperplasia of koi carp (cyprinus carpio) in northern california (usa). papillomas were found principally on the caudal regions of the fish including the fins. the growths occurred most commonly in the fall and winter among populations of captive carp. infected epidermal cells were characterized by greatly enlarged nuclei depleted of chromatin but with thickened nuclear membranes. numerous virions were detected in infected cells. herpesvirus nuc ... | 1990 | 2174469 |
| histamine in the retina: recent progress and perspectives. | this article presents some new findings, and shortly surveys recently published data, on histamine (ha) in the retina of several vertebrates (carp, hen, rabbit, rat, guinea pig, cow, man). analysis of various parameters (ha level, activity of histidine decarboxylase and ha-methyltransferase, release and uptake of ha, 3h-mepyramine binding, effect of ha on camp accumulation, diurnal variations) in vertebrate retina and brain shows that histaminergic systems in these sites are comparable, although ... | 1990 | 2196761 |
| immunological and ultrastructural characterization of true histiocytic lymphoma in the northern pike, esox lucius l. | an epidemic of a malignant neoplasm occurs in northern pike, esox lucius l., from the aland islands of finland. the neoplasm is morphologically similar to other pike hemic tumors reported in other areas of the world. pike normal tissues showed evolutionary conservation with the mammalian intermediate filament proteins cytokeratin, desmin, vimentin, neurofilament protein, and glial fibrillary acidic protein; tumor cells are positive for vimentin, suggesting that the neoplasm is of mesenchymal ori ... | 1990 | 2201440 |
| close tip-to-tip contacts between dendrites of transient amacrine cells in carp retina. | in isolated retinas of the carp (cyprinus carpio) placed receptor side up in a plastic chamber, a subclass of amacrine cells, generating a fast on-off transient response to spot and annular light stimuli, were intracellularly recorded and injected with a fluorescent dye, lucifer yellow (ly). after brief fixation of the same preparations in aldehyde solution, the retinas were wholemounted vitreous side up in a tissue chamber. under a fluorescence microscope, one ly-injected cell and several dye-c ... | 1990 | 2216051 |
| growth, morphology and division of flagellates of the genus trypanoplasma (protozoa, kinetoplastida) in vitro. | nine strains of trypanoplasms were grown in axenic culture. cultures of trypanoplasma borreli laveran and mesnil, 1901 from fish hosts blicca bjoerkna, cyprinus carpio, scardinius erythrophthalmus and tinca tinca and of t. guerneyorum minchin, 1909 from esox lucius and trypanoplasma spp. from the leech piscicola geometra were maintained in biphasic blood-agar medium snb-9 supplemented with vitamins and antibiotics. in culture, the flagellates transformed into smaller, elongated stages with a lit ... | 1990 | 2217113 |
| structure of oncomodulin refined at 1.85 a resolution. an example of extensive molecular aggregation via ca2+. | the crystal structure of oncomodulin, a 12,000 mr protein isolated from rat tumours, has been determined by molecular replacement using the carp parvalbumin structure as a starting model. refinement was performed by cycles of molecular fitting and restrained least-squares, using area-detector intensity data to 1.85 a resolution. for the 5770 reflections in the range 6.0 to 1.85 a, which were used in the refinement, the crystallographic r-factor is 0.166. the refined model includes residues 2 to ... | 1990 | 2231727 |
| the effect of temperature on catalytic function of glyceraldehyde-3-phosphate dehydrogenase from muscle of pig and carp cyprinus carpio. | 1. the temperature dependence of the kinetics of glyceraldehyde-3-phosphate dehydrogenase from white muscle of carp and skeletal muscle of pig was examined. 2. the km values of carp muscle enzyme were stable over the temperature range 5-35 degrees c, but increased for pig muscle enzyme with increasing temperature. 3. the arrhenius plot for pig muscle enzyme is linear but non-linear for carp muscle enzyme. 4. the differences indicate that glyceraldehyde-3-phosphate dehydrogenase from white carp m ... | 1990 | 2253480 |
| comparison of the gamma-crystallins isolated from eye lenses of shark and carp. unique secondary and tertiary structure of shark gamma-crystallin. | gamma-crystallin isolated from the shark of cartilaginous fishes was compared with the cognate gamma-crystallin from the carp of bony fishes. distinct differences in amino acid compositions, primary, secondary and tertiary structures were found. the most salient features of shark gamma-crystallin lie in the fact that this crystallin possessed a significant alpha-helical structure in the peptide backbone as revealed by circular dichroism study, in contrast to those orthologous gamma-crystallins f ... | 1990 | 2261977 |
| allergy to different fish species in cod-allergic children: in vivo and in vitro studies. | the presence of a positive clinical history and skin test (st) results for 17 fish species (anchovy, bass, carp, dogfish, eel, gilthead, mackerel, mullet, perch, red mullet, salmon, sardine, sole, tench, toothed gilthead, trout, and tuna) were investigated in 20 children with cod-positive clinical history, st, and rast, and in 40 children positive to one or more foods different from cod (cow's milk, chicken egg white, peanut, and tomato). in cod-positive children, positive clinical history (60%) ... | 1990 | 2262645 |
| immunological evidence that the neural adhesion molecule l1 is expressed in fish brain and optic nerve: possible association with optic nerve regeneration. | in the mammalian peripheral nervous system (pns), expression of the neural adhesion molecule l1 on schwann cells and neurons has been correlated with axonal growth during development and regeneration. the present study was undertaken to examine whether a similar correlation exists between a lesion-induced increase of l1 expression and regenerative capacity in the central nervous system (cns). the fish optic nerve was used as a model for a successfully regenerating region of the cns. immunochemic ... | 1990 | 2265355 |
| antigen-capture elisa for viral haemorrhagic septicaemia virus serotype i. | an antigen-capture elisa for viral haemorrhagic septicaemia virus serotype i (vhsv i) was developed. the assay employs two monoclonal antibodies (mab) directed against distinct epitopes of the viral envelope glycoprotein (gp). the antigen bound by the capture mab (a17) was detected by addition of a second mab (l7) conjugated to horseradish peroxidase, followed by addition of the enzyme substrate. the technique is highly sensitive, enabling detection of the virus at a protein concentration as low ... | 1990 | 2266147 |
| [ultrastructure of the renal corpuscle of fresh water fishes]. | by means of light (semithin slices) and electron microscopes structural organization of the corpuscle and glomerulus of the capsule has been studied in freshwater fishes--carp (cyprinus carpio) and crucian carp (carassius carassius). the diameter of their glomeruli are in the carp from 80.8 +/- 1.4 mcm up to 59.8 +/- 2.4 mcm and in the crucian carp--from 86.4 +/- 1.8 mcm up to 69.25 +/- 0.2 mcm. between these dimensions there are significant differences. components of the external wall of the ca ... | 1990 | 2268195 |
| the complete amino acid sequence of prolactin from the sea turtle (chelonia mydas). | the complete amino acid sequence of prolactin (prl) from a reptile, the sea turtle (chelonia mydas), was determined for the first time. sequence analysis was performed on fragments obtained from cleavage of intact and performic acid-oxidized hormone with lysyl endopeptidase, staphylococcus aureus protease, and o-iodosobenzoic acid employing manual edman degradation. the sea turtle prl consists of 198 amino acid residues with three disulfide linkages formed between residues 4-11, 58-173, and 190- ... | 1990 | 2289679 |
| population dynamics and interrelationships of some dactylogyrus and gyrodactylus species on cyprinus carpio. | during 1986/87, 922 carps have been examined for ectoparasitic helminths. altogether 6 species of gyrodactylus and 4 species of dactylogyrus were found. the most frequent parasite on the gills was d. extensus; it shows a great tolerance to the water temperature with two peaks of infestation between 2 and 9 degrees c. additionally, relationships between the seasonal dynamics of the invasion cycle of d. extensus and d. anchoratus were recognized. in addition to dactylogyrus 2 species of gyrodactyl ... | 1990 | 2291498 |
| the role of tubificid oligochaetes in the transmission of goussia carpelli. | tubifex tubifex and limnodrilus hoffmeisteri, fed intestinal tissue of carp containing oocysts of goussia carpelli, produced infections in laboratory-reared carp. sporocysts ingested by the tubificids released the sporozoites that were found to be motile in the intestinal contents and incorporated in intestinal cells of the oligochaetes. tubificids remained infective for carp at least up to 57 days postexposure (pe). tubificids were also able to transmit g. carpelli to carp that had recovered fr ... | 1990 | 2299515 |
| inhibition of glutathione reductase by oncomodulin. | evidence for a specific interaction between oncomodulin and glutathione reductase is presented. glutathione reductase (ec 1.6.4.2) isolated from either the bovine intestinal mucosa or the rat liver was bound in a ca2(+)-dependent manner to oncomodulin which was covalently attached to sepharose. in addition, glutathione reductase was able to catalyze the reduction of the disulfide-linked dimer of oncomodulin. the interaction of these proteins could also be indirectly demonstrated by monitoring gl ... | 1990 | 2306116 |
| in vivo effect of the organophosphorus insecticide trichlorphon on the immune response of carp (cyprinus carpio). i. effect of contamination on antibody production in relation to residue level in organs. | the organophosphorus insecticide trichlorphon was tested at medium (10 and 20 ppm) and high concentrations (20,000 ppm) on antibody production against yersinia ruckeri vaccine. no statistically significant effect was observed on humoral response or on hematocrit. contamination of organs was analyzed by acetylcholinesterase activity. at 20,000 ppm only liver and brain were highly contaminated; spleen and lymphocytes were not significantly contaminated. at 20 ppm no residue was found in organs. | 1990 | 2311566 |
| in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response. | the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ... | 1990 | 2311567 |
| light and electron microscopic observations on the swimbladder stages of sphaerospora renicola, a parasite of carp (cyprinus carpio). | the developmental cycle of stages of sphaerospora renicola, proliferating in the walls of the swimbladder in carp fry and responsible for swimbladder inflammation (= sbi stages) is described. the initial stage, a primary cell containing two secondary cells, grows in size while the secondary cells divide and produce two tertiary cells each by endogenous division. after a supposed disintegration of the grown primary cell, the cell triplets are released to repeat the cycle. the cell divisions are a ... | 1990 | 2315282 |
| mitogenic activity from trout embryos. | an extract of 21-day rainbow trout embryos stimulated growth of several piscine cell lines in the absence of added serum. established lines from trout (rtg-2 and ste-137), salmon (chse-214), carp (epc), and goldfish (car) and early-passage cells initiated from trout embryos grew in serum-free medium containing the embryo extract. in addition the extract was sufficient for maintaining long-term cultures of chse-214 cells for several months through a minimum of 20 passages (approximately 50 popula ... | 1990 | 2333296 |
| gulonolactone oxidase is missing in teleost fish. the direct spectrophotometric assay. | data in the literature imply that some fish species evolved with the capacity to synthesize ascorbic acid. gulonolactone oxidase activity has been reported in kidney and/or liver tissues. however, it is shown here that this microsomal enzyme activity is missing in common carp hepatopancreas and kidney, whereas high activity was confirmed in pigeon kidney, rat liver, bovine liver and amphibian (xenopus) kidney tissues. a new assay using either the whole tissue homogenate or microsomes solubilized ... | 1990 | 2340104 |
| functional analysis of elements affecting expression of the beta-actin gene of carp. | regulatory regions of the beta-actin gene of the common carp (cyprinus carpio) have been examined by linking upstream, 5'-flanking sequences and regions of the first intron to a bacterial chloramphenicol acetyltransferase (cat) reporter gene. by analysis of the mrna products and encoded cat activity, we have identified four putative regions that influence expression: (i) a negative regulatory region 2,300 to 1,100 base pairs (bp) ahead of the gene; (ii) a proximal promoter element, containing th ... | 1990 | 2355913 |
| effect of levamisole on the lymphocyte and macrophage activity in carp (cyprinus carpio). | levamisole, an anthelminthic drug which is often used in cattle, is known for its immunomodulatory effect in several species. in the present work we investigated the in vitro effects of different concentrations of levamisole on lymphocyte proliferation with mitogen in carp. at low concentrations, ie 12.5 to 0.1 micrograms/ml, levamisole stimulated proliferation with phytohemagglutinin p (pha-p) and con a, had no effect at medium concentrations and acted as a suppressor at high concentrations, ie ... | 1990 | 2360777 |
| effect of beta-naphthoflavone and mcpa on liver and kidney drug-metabolizing enzymes from the carp, cyprinus carpio. | the effects of beta-naphthoflavone (beta-nf) and a chlorophenoxyacetic acid herbicide (mcpa) on hepatic and renal monooxygenase activities and conjugating enzymes from immature carp (cyprinus carpio) were studied. beta-nf increased hepatic monooxygenase activities but the patterns of differential induction generally obtained in rat liver microsomes with two series of homologous substrates, alkoxycoumarins and alkylresorufins, were not found to be similar in carp liver microsomes. on the other ha ... | 1990 | 2364910 |
| lymphomyeloid cells, susceptibility to erythrodermatitis of carp and bacterial antigens. | pronounced changes occur in the kidneys carp injected with extracellular product (ecp), lipopolysaccharide (lps) of a. salmonicida subsp. salmonicida, freund's complete adjuvant (fca). ecp caused 50-70% decrease in macrophages and neutrophils, whereas blasts and lymphocyte-like cells increased fca-elicited increased percentages of blasts and a left shift of neutrophils after lps injection. after both ecp and fca injections, the density of major leucocyte types and red blood cells (rbc) in the ki ... | 1990 | 2369967 |
| [clinico-parasitological research in a mixed focus of clonorchiasis and intestinal nematodiasis in hanamnin province (the socialist republic of vietnam)]. | the authors described the clonorchiasis focus in the vietnam province at the seaside delta of the red river where the aforementioned invasion was accompanied with multiple invasions with intestinal nematodes. clonorchiasis infestation rate was 28.4% ascaridiasis, 50.5%, trichocephaliasis, 21.3% ancylostomiasis, 7.0%. clonorchiasis invasion was the result of consumed uncooked carp fish in the salad. it was revealed that the fish was reared in the ponds near the settlements and was fed untreated h ... | 1990 | 2377136 |
| a comparative study of the unfolding thermodynamics of vertebrate metmyoglobins. | differential scanning microcalorimetry (dsc) of horse, rat, opossum, raccoon, carp, and armadillo metmyoglobins at alkaline ph gave data that fit the two-state unfolding model well. monte carlo studies were used to assess the impact of truncating dsc scans on the reliability of the calculated results when aggregation exotherms overlapped the unfolding endotherm at the high-temperature end of the scan. the dsc estimates for the conformational free energy at ph 8 and 298 k are compared to earlier ... | 1990 | 2378866 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| isolation of carp genes encoding major histocompatibility complex antigens. | in the evolution of the adaptive immune system unique to vertebrates, teleost fish occupy the critical position. this is the most primitive class of lower vertebrates in which the capacity for acute allograft rejections can be demonstrated, thus suggesting the presence of major histocompatibility complex (mhc) antigens and, therefore, t cells. here, we report the identification of two putative mhc-antigen-encoding sequences in the carp cyprinus carpio. one, identified as tlai alpha-1, had reason ... | 1990 | 2395879 |
| distribution of gaba-like immunoreactivity in myenteric plexus of carp, frog and chicken. | the distribution of gaba-like immunoreactivity was studied by means of indirect immunocytochemical methods in some lower vertebrate species (carp, frog, chicken). an immunoreactive network was revealed in the myenteric plexus of the alimentary canal of carp. gaba-positive nerve cells were attached closely to the fibres in the stomach. in other gut regions immunostained neurons were less frequent. immunoreactive fibres often formed baskets on the surfaces of immunonegative neurons along the whole ... | 1990 | 2401638 |
| [the effect of silver carp oil on the blood pressure and lipids of spontaneously hypertensive rats]. | 1990 | 2402274 | |
| hepatic 5'-monodeiodinase activity in teleosts in vitro: a survey of thirty-three species. | the in vitro hepatic 5'-monodeiodination of thyroxine (t4) to triiodothyronine (t3) in oreochromis mossambicus, channa striata, clarias batrachus, cyprinus carpio and oxyeleotris marmorata was found to be time, ph and temperature dependent, and related to the amount of substrate (t4) and homogenate introduced into the reaction vessel, in a manner which was consistent with menton-michaelis kinetics, and thus indicative of an enzyme-regulated process. dithiothreitol introduced into the reaction ve ... | 1990 | 24221892 |
| turnover of serotonin in brain of an anoxia-tolerant vertebrate, the crucian carp. | serotonin (5-ht) is a neurotransmitter in the vertebrate brain. estimates of 5-ht turnover times range between 1 h (mammals) and 10 h (goldfish). synthesis and catabolism of 5-ht are dependent on molecular oxygen, hence, 5-ht turnover comes to a halt during anoxia. nevertheless, a few vertebrates, such as the crucian carp (carassius carassius l.), tolerate anoxia for several weeks. to assess the ability of the crucian carp brain to tolerate a stop in 5-ht turnover during anoxia, it is essential ... | 1990 | 1694412 |
| long-term anoxia in crucian carp: changes in the levels of amino acid and monoamine neurotransmitters in the brain, catecholamines in chromaffin tissue, and liver glycogen. | crucian carp (carassius carassius l.), which are extremely anoxia-tolerant, were exposed to 17 days of anoxia at 8 degrees c. one group of fish was transferred to normoxic water for 1-8 h immediately after the anoxic period. all the eight amino acids measured in brain (including four putative neurotransmitters) were more or less strongly affected by anoxia. gamma-aminobutyric acid (gaba) displayed a nearly fivefold increase during anoxia. it is hypothesized that the increased level of this inhib ... | 1990 | 1972385 |
| conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species. | blood cells from baltic salmon, salmo salar, three-spined stickleback, gasterosteus aculeatus, eel pout, zoarces viviparus, crucian carp, carassius carassius, african catfish, clarias gariepinus, and reedfish, calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (oha) or 11-ketoandrostenedione (oa). in all fish there was conversion of oa to 11-ketotestosterone (ot), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. on ... | 1990 | 2295425 |
| thermal acclimation and muscle contractile properties in cyprinid fish. | after several weeks of cold acclimation, the swimming performance of some fish is increased at low temperatures and decreased at high temperatures. the temperature compensation of locomotory activity involves changes in central patterns of muscle fiber recruitment and in the properties of the peripheral nervous system and muscle tissues. in some freshwater fish belonging to the family cyprinidae, including the goldfish (carassius auratus), the common carp (cyprinus carpio), and the roach (rutilu ... | 1990 | 2143635 |
| seasonal changes in serum gonadotropin, testosterone, 11-ketotestosterone, and estradiol-17 beta levels and their relation to tumor burden in gonadal tumor-bearing carp x goldfish hybrids in the great lakes. | the hybrids of carp (cyprinus carpio) and goldfish (carassius auratus) collected from the lower great lakes between 1978 and 1981 exhibited a high frequency of gonadal neoplasms and apparent sterility, conditions which were rare in the parental species. a pronounced hyperplasia of gonadotropic cells in hybrids resulted in the accumulation of large stores of biologically active gonadotropin (gth) in the pituitary gland. serum gth levels were generally higher in hybrids than in carp collected from ... | 1990 | 2307342 |