Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cloning and nucleotide sequence of a full-length cdna encoding ascaris suum phosphofructokinase. | the nucleotide sequence of a full-length cdna encoding phosphofructokinase (pfk) enzyme from the parasitic nematode ascaris suum was determined. the entire sequence of 2,653 bases comprises a single open reading frame of 2,452 bases and a noncoding region of 201 bases after the stop codon. the mature protein contains 812 amino acids and has a molecular mass of 90,900 da. the amino acid sequences of several peptides derived from the purified protein show excellent correspondence with the translat ... | 2005 | 16108551 |
| enzyme-linked immunosorbent assay and western blot antibody determination in sera from patients diagnosed with different helminthic infections with anisakis simplex antigen purified by affinity chromatography. | an evaluation of the sensitivity and the specificity of the anisakis simplex antigens purified by affinity chromatography was performed using sera from patients diagnosed with anisakis sensitisation and sera from patients previously diagnosed with different helminthic infections. only the sera of the patients diagnosed with schistosoma mansoni or onchocerca volvulus parasitic infections were negative against the a. simplex antigen and its purified fractions (pak antigen: a. simplex antigen purif ... | 2005 | 16113872 |
| investigating helminth eggs and salmonella sp. in stabilization ponds treating septage. | sludge management arises as a relevant problem after being accumulated in primary ponds of septage treatment plants. one of the most attractive options for sludge disposal is its use in agriculture and then specific guidelines regarding hygienic quality must be fulfilled. this study aimed at evaluating the storage time needed to inactivate ascaris eggs and salmonella in sludge accumulated in a primary pond treating septage. raw septage exhibited very low concentrations of viable ascaris eggs, th ... | 2005 | 16114689 |
| inactivation of ascaris suum and poliovirus in biosolids under thermophilic anaerobic digestion conditions. | there is considerable interest in the united states in production of class a (low pathogen content) biosolids from the treatment of municipal wastewater sludge. current requirements imposed by the u.s. environmental protection agency make it difficult for thermophilic anaerobic digestion, in its simplest process configurations, to achieve class a status. in particular, the time-temperature requirements necessitate long batch treatment times at temperatures associated with thermophilic anaerobic ... | 2005 | 16124318 |
| expression and bioactivity of allatostatin-like neuropeptides in helminths. | allatostatins are the largest family of known arthropod neuropeptides. to date more than 150 different arthropod type-a allatostatins have been identified and are characterized by the c-terminal signature, (y/f)xfg(l/i)amide. using specific allatostatin antisera, positive immunoreactivity has been identified within the central and peripheral nervous systems of the flatworm (platyhelminth) procerodes littoralis and the roundworm (nematode) panagrellus redivivus. comparative analyses of the allato ... | 2005 | 16185693 |
| characterization of a novel filarial serine protease inhibitor, ov-spi-1, from onchocerca volvulus, with potential multifunctional roles during development of the parasite. | a novel filarial serine protease inhibitor (spi) from the human parasitic nematode onchocerca volvulus, ov-spi-1, was identified through the analysis of a molting third-stage larvae expressed sequence tag dataset. subsequent analysis of the expressed sequence tag datasets of o. volvulus and other filariae identified four other members of this family. these proteins are related to the low molecular weight spis originally isolated from ascaris suum where they are believed to protect the parasite f ... | 2005 | 16186127 |
| a survey of gastrointestinal pig parasites on free-range, organic and conventional pig farms in the netherlands. | the prevalence of gastrointestinal parasites in suckling piglets, weaners, fattening pigs and sows was studied from november 2001 to october 2002 on 16 free-range farms (frf), 11 organic farms (of) and 9 conventional farms (cf) by means of faecal examinations of composite samples. each farm was visited four times with a 3-month interval. infections with coccidia were found on 43.8% of the frf, 90.9% of the of and 66.7% of the cf. sows had the highest prevalence, particularly on frf (87.5%) and o ... | 2005 | 16195935 |
| parasitic zoonoses in papua new guinea. | relatively few species of zoonotic parasites have been recorded in humans in papua new guinea. a greater number of potentially zoonotic species, mostly nematodes, occur in animals but are yet to be reported from humans. protozoa is the best represented group of those infecting man, with giardia duodenalis, cryptosporidium parvum, cyclospora cayetanesis, toxoplasma gondii, sarcocystis spp., entamoeba polecki, balantidium coli and, possibly, blastocystis hominis. the only zoonotic helminths infect ... | 2005 | 15831107 |
| collection of exhaled breath condensate and analysis of hydrogen peroxide as a potential marker of lower airway inflammation in cats. | the objective of this study was to describe a standardised and non-invasive method for exhaled breath condensate (ebc) collection in cats and to test whether determination of hydrogen peroxide (h(2)o(2)) in ebc might be used as marker of lower airway inflammation. the technique of barometric whole body plethysmography for cats was combined with a system to condense the effluent air from the plethysmograph, allowing simultaneous ebc collection and respiratory pattern measurement. h(2)o(2) was det ... | 2005 | 15848781 |
| cecropin p1 and novel nematode cecropins: a bacteria-inducible antimicrobial peptide family in the nematode ascaris suum. | cecropin p1 was first identified as a mammalian antimicrobial peptide isolated from the pig intestine. much research aimed at characterizing this peptide has been reported. recently, the workers who discovered the peptide corrected their original conclusion, and confirmed that this peptide originates in fact from the pig intestinal parasitic nematode, ascaris suum. in the present study, we carried out a semi-exhaustive search for bacteria-inducible transcripts in a. suum by the cdna subtraction ... | 2005 | 15850460 |
| effects of inhaled brevetoxins in allergic airways: toxin-allergen interactions and pharmacologic intervention. | during a florida red tide, brevetoxins produced by the dinoflagellate karenia brevis become aerosolized and cause airway symptoms in humans, especially in those with pre-existing airway disease (e.g., asthma). to understand these toxin-induced airway effects, we used sheep with airway hypersensitivity to ascaris suum antigen as a surrogate for asthmatic patients and studied changes in pulmonary airflow resistance (r(l) after inhalation challenge with lysed cultures of k. brevis (crude brevetoxin ... | 2005 | 15866776 |
| host immune reactions and worm kinetics during the expulsion of ascaris suum in pigs. | pigs single inoculated with ascaris suum eggs expel the majority of larvae between days 14 and 21 post inoculation (p.i.), but the role of the immune system in expulsion is unclear. to investigate the dynamics of immune responses before, during and after the expulsion of a. suum larvae, pigs inoculated with 10 000 a. suum eggs were sequentially necropsied. ascaris suum gradually moved distally from days 10-14 p.i. and only a few larvae were left by day 21 p.i. pronounced increases in mucosal a. ... | 2005 | 15882234 |
| contrasting effects of acute and chronic gastro-intestinal helminth infections on a heterologous immune response in a transgenic adoptive transfer model. | we have previously found that co-immunisation with ovalbumin (ova) and the body fluid of the helminth ascaris suum inhibited an ova-specific delayed type hypersensitivity (dth) response by reducing ova-specific cd4+ t lymphocyte proliferation via an il-4 independent mechanism. in the present study, we determined whether parasite infections themselves could induce similar changes to peripheral immunisation by examining the modulation of ova-specific immune responses during acute and chronic helmi ... | 2005 | 15893319 |
| establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines. | mast cells (mcs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. they are the pivotal mediating cells of allergic reactions. in addition, in mice, mcs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. whereas the biology of rodent and human mcs has been extensively studied using in vitro derived populations, the role of mcs in pigs has not yet been evaluated, given the very low availability of ... | 2005 | 15896811 |
| unusual usage of wobble modifications in mitochondrial trnas of the nematode ascaris suum. | to understand the decoding property of nematode mitochondrial trnas with unusual secondary structures, post-transcriptional modifications at wobble positions of ascaris suum mitochondrial trnas corresponding to two-codon families ending with a purine were analyzed. 5-carboxymethylaminomethyluridine (cmnm(5)u) was identified at the wobble positions of trna(lys), trna(glu) and trna(gln), while 5-carboxymethylaminomethyl-2-thiouridine (cmnm(5)s(2)u) was present in trna(uaa)(leu)andtrna(trp). in mos ... | 2005 | 15907479 |
| convergent evolution of invertebrate defensins and nematode antibacterial factors. | antibacterial factors (abfs) are secreted polypeptides that have an important role in the innate immune system of nematodes. comparison of these polypeptides revealed similarity in bioactivity, protein sequence and 3d structure, suggesting that they originated from a common ancestor. comparison of gene organization of nematode abf genes revealed that all except one contain a phase 0 intron at a conserved location. the intron phase and location are congruent with the postulated intron gain rules, ... | 2005 | 15914006 |
| endogenous morphine and parasitic helminthes. | the current paper summarizes the findings on endogenous morphine isolated by hplc and characterized by mass spectroscopy in schistosoma mansoni, dracunculus medinensis and ascaris suum. morphine-6-glucuronide (m6g) has also been found by hplc and confirmed by mass spectroscopy in dracunculus medinensis and ascaris suum. in addition, a morphine like substance has been isolated from trichinella spiralis by hplc and mice infected with trichinella spiralis show a naloxone reversible analgesia. we di ... | 2005 | 15917729 |
| single-channel properties of n- and l-subtypes of acetylcholine receptor in ascaris suum. | we are interested in the properties of the target site of cholinergic anti-nematodal drugs for therapeutic reasons. the target receptors are ligand-gated ion channels that have different subtypes, and each subtype may have a different pharmacology. in a contraction assay using the parasitic nematode ascaris suum, our laboratory has identified several subtypes, including an n-subtype, preferentially activated by nicotine, and an l-subtype, preferentially activated by levamisole. here we use patch ... | 2005 | 15950977 |
| isolation and physiochemical properties of protein-rich nematode mitochondrial ribosomes. | in the present study, mitochondrial ribosomes of the nematode ascaris suum were isolated and their physiochemical properties were compared to ribosomes of escherichia coli. the sedimentation coefficient and buoyant density of a. suum mitochondrial ribosomes were determined. the sedimentation coefficient of the intact monosome was about 55 s. the buoyant density of formaldehyde-fixed ribosomes in cesium chloride was 1.40 g/cm(3), which suggests that the nematode mitoribosomes have a much higher p ... | 2005 | 15966747 |
| mass spectrometric map of neuropeptide expression in ascaris suum. | a mass spectrometric method was used for the localization and sequence characterization of peptides in the nervous system of the parasitic nematode ascaris suum. mass spectrometric techniques utilizing maldi-tof, maldi-tof/tof, and maldi-ft instruments were combined with in situ chemical derivatization to examine the expression of known and putative neuropeptides in the a. suum nervous system. this first attempt at peptidomic characterization in a. suum mapped the expression of 39 neuropeptides, ... | 2005 | 15973679 |
| vaccination with recombinant ascaris suum 24-kilodalton antigen induces a th1/th2-mixed type immune response and confers high levels of protection against challenged ascaris suum lung-stage infection in balb/c mice. | previous studies have shown that antigens from various life-cycle stages of ascaris suum can induce host-protective immunity against challenge infections with infective eggs of a. suum. this study evaluated whether escherichia coli-expressed recombinant 24-kda antigen from a. suum (ras24) was a suitable vaccine candidate for the control of ascaris infections by examining its performance in a mouse model. immunization of balb/c mice in three consecutive doses with ras24 in freund's complete adjuv ... | 2005 | 15998515 |
| modulation of murine experimental asthma by ascaris suum components. | we have recently isolated two distinct components from ascaris suum adult worms with different effects on the immune system: the allergenic protein of a. suum (apas-3), which induces ige antibody production, and suppressive protein of a. suum (pas-1), which inhibits humoral and cellular immune responses induced by unrelated antigens. in this study, we investigated the immunomodulatory effect of pas-1 on a murine model of asthma induced by apas-3. | 2005 | 16008672 |
| msp domain protein-1 from ascaris suum and its possible role in the regulation of major sperm protein-based crawling motility. | nematode sperm utilize a crawling motility based on a nematode sperm-specific cytoskeletal protein called the major sperm protein (msp). although msp has no similarity to actin in sequence or structure, the motility mediated by these two proteins is nearly indistinguishable at a phenotypic level. as with the traditional actin cytoskeleton, the central component of msp-based motility (msp) interacts with accessory proteins that regulate polymerization and depolymerization and play a key role in c ... | 2005 | 16026867 |
| molecular cloning and partial characterization of a nematode-specific 24 kda protein from ascaris suum. | the cloning and molecular characterization of a cdna encoding ascaris suum 24 kda antigen (as24) are described. the cdna sequence consists of 853 bp with an open reading frame coding for a protein of 147 amino acids with an inferred signal peptide of 19 amino acids. the predicted molecular mass and pi were 16 kda and 8.35 respectively. the endogenous protein in adult a. suum was 24 kda with the expected pi. a search of the public databases revealed over 50% homology with proteins from filarial p ... | 2005 | 15700764 |
| localized multigene expression patterns support an evolving th1/th2-like paradigm in response to infections with toxoplasma gondii and ascaris suum. | human infectious diseases have been studied in pigs because the two species have common microbial, parasitic, and zoonotic organisms, but there has been no systematic evaluation of cytokine gene expression in response to infectious agents in porcine species. in this study, pigs were inoculated with two clinically and economically important parasites, toxoplasma gondii and ascaris suum, and gene expression in 11 different tissues for 20 different swine th1/th2-related cytokines, cytokine receptor ... | 2005 | 15664955 |
| msp dynamics drives nematode sperm locomotion. | most eukaryotic cells can crawl over surfaces. in general, this motility requires three sequential actions: polymerization at the leading edge, adhesion to the substrate, and retraction at the rear. recent in vitro experiments with extracts from spermatozoa from the nematode ascaris suum suggest that retraction forces are generated by depolymerization of the major sperm protein cytoskeleton. combining polymer entropy with a simple kinetic model for disassembly we propose a model for disassembly- ... | 2005 | 15665134 |
| brief application of af2 produces long lasting potentiation of nachr responses in ascaris suum. | resistance of parasitic nematodes to the cholinergic anthelmintic levamisole is associated with a reduction in the proportion of time that acetylcholine receptor ion-channels are in the open state decreasing the response of nematode parasites to the drug. here we examine electrophysiological and contractile responses to acetylcholine and the cholinergic agonist, levamisole, in ascaris suum muscle looking for a pharmacological approach that may be developed to increase the response to cholinergic ... | 2005 | 15610819 |
| the development of a mouse model to explore resistance and susceptibility to early ascaris suum infection. | ascaris suum and ascaris lumbricoides exhibit an over-dispersed frequency distribution in their host populations in both the adult and larval stages. the impact of host factors on this observed distribution is still poorly understood and difficult to investigate in the natural host populations. the use of a mouse model has been supported by the observations that the larval migratory pattern, in this host, mimics the pattern observed in the pig. we explored the extrinsic factors that might affect ... | 2006 | 16209722 |
| cross-species rnai: selected ascaris suum dsrnas can sterilize caenorhabditis elegans. | 2006 | 16388861 | |
| drug transport mechanisms in helminth parasites: passive diffusion of benzimidazole anthelmintics. | anthelmintic molecules must reach their receptors inside target parasites to exert the pharmacological effect. available data suggest that the main route of entry of antiparasitic drugs into helminth parasites would be through their external surface. however, it is unclear if trans-tegumental/cuticular penetration is the most important way of entry of benzimidazole (bzd) anthelmintics into their target parasites compared to oral ingestion. the relative involvement of active and passive transport ... | 2006 | 16430886 |
| dissecting ascaris glycosphingolipids for immunomodulatory moieties--the use of synthetic structural glycosphingolipid analogues. | we have previously shown glycosphingolipids of ascaris suum to have phosphorylcholine (pc) and non-pc immunomodulatory moieties. in the present study we further investigated the nature of the immunomodulatory moieties by employing three synthetic glycosphingolipids each possessing features of the original molecule to examine effects on macrophage and dendritic cell (dc) cytokine production and surface co-stimulatory molecule expression. compound 2, which lacked pc but contained ceramide, had no ... | 2006 | 16441504 |
| the complete mitochondrial genome of anisakis simplex (ascaridida: nematoda) and phylogenetic implications. | we determined the nucleotide sequence of the complete mitochondrial genome of the nematode species anisakis simplex. the genome is circular, 13,916 bp in size and conforms to the general characteristics of nematode mitochondrial dnas. the gene arrangement of a. simplex is the same as that of ascaris suum and almost identical to those of rhabditid species with a minor exception concerning the relative position of the at-rich and non-coding regions and radically different from those of spirurid sp ... | 2006 | 16442542 |
| deep-breath frequency in bronchoconstricted monkeys (macaca fascicularis). | deep-breath frequency has been shown to increase in spontaneously obstructed asthmatic subjects. furthermore, deep breaths are known to be regulated by lung rapidly adapting receptors, yet the mechanism by which these receptors are stimulated is unclear. this study tested the hypothesis that deep-breath frequency increases during experimentally induced bronchoconstriction, and the magnitude of the increased deep-breath frequency is dependent on the method by which bronchoconstriction is induced. ... | 2006 | 16467390 |
| fertilization state of ascaris suum determined by electrorotation. | electrorotation is a non-invasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. the first detailed electrorotation study of the egg (ovum) of a parasitic nematode, namely ascaris suum is described to show that electrorotation can rapidly differentiate between fertilized and non-fertilized eggs. support for this conclusion is by optical microscopy of egg morphology, and also from modelling of the electrorotational response. mode ... | 2006 | 16469169 |
| influence of season and host age on wild boar parasites in corsica using indicator species analysis. | the indicator value (ind val) method which combines measures of fidelity and specificity has been used in a study on wild boar parasites in corsica during 2001-2003. because of its resilience to changes in abundance, indval is a particularly effective tool for ecological bioindication. the ind val method showed how season can influence the occurrence of parasite species in the wild boar, and also identified parasites as bioindicators relative to host age. the randomization test identified five p ... | 2006 | 16469171 |
| optimum activity of the phosphofructokinase from ascaris suum requires more than one metal ion. | phosphofructokinase (pfk) catalyzes the phosphorylation of fructose 6-phosphate (f6p) to give fructose 1,6-bisphosphate (fbp) using mgatp as the phosphoryl donor. as the concentration of mg(2+) increases above the concentration needed to generate the mgatp chelate complex, a 15-fold increase in the initial rate was observed at low mgatp. the effect of mg(2+) is limited to v/k(mgatp), and initial rate studies indicate an equilibrium-ordered addition of mg(2+) before mgatp. isotope partitioning of ... | 2006 | 16475835 |
| measurement of one-bond 13calpha-1halpha residual dipolar coupling constants in proteins by selective manipulation of calphahalpha spins. | we have developed new 2d and 3d experiments for the measurement of c(alpha)-h(alpha) residual dipolar coupling constants in (13)c and (15)n labelled proteins. two experiments, 2d (hnco)-(j-ca)nh and 3d (hn)co-(j-ca)nh, sample the c(alpha)-h(alpha) splitting by means of c(alpha) magnetization, while 2d (j-hacaco)nh and 3d j-ha(caco)nh use h(alpha) magnetization to achieve a similar result. in the 2d experiments the coupling evolution is superimposed on the evolution of the (15)n chemical shifts a ... | 2006 | 16495100 |
| a case of ascaris suum visceral larva migrans diagnosed by using a. suum larval excretory-secretory (es) antigen. | a 42-y-old female presented with common cold-like symptoms. laboratory data showed mild liver dysfunction together with peripheral blood eosinophilia. she was suspected of having a helminthic infection, however parasite eggs or larvae were not detected by repeated stool examinations. eventually she transpired to have a high igg antibody titer against excretory-secretary (es) antigen of ascaris suum larvae, but not of toxocara canis larvae, suggesting that she had been suffering from visceral lar ... | 2006 | 16500788 |
| inactivation of single-celled ascaris suum eggs by low-pressure uv radiation. | intact and decorticated single-celled ascaris suum eggs were exposed to uv radiation from low-pressure, germicidal lamps at fluences (doses) ranging from 0 to 8,000 j/m2 for intact eggs and from 0 to 500 j/m2 for decorticated eggs. with a uv fluence of 500 j/m2, 0.44-+/-0.20-log inactivation (mean+/-95% confidence interval) (63.7%) of intact eggs was observed, while a fluence of 4,000 j/m2 resulted in 2.23-+/-0.49-log inactivation (99.4%). (the maximum quantifiable inactivation was 2.5 log units ... | 2006 | 16517669 |
| pas-1, a protein affinity purified from ascaris suum worms, maintains the ability to modulate the immune response to a bystander antigen. | helminth infections and parasite components have potent immunomodulatory effects on a host's immune system. in the present study, we investigated the effect of pas-1, a protein component of ascaris suum adult worms recognized by a monoclonal antibody (maip-1), on humoral and cell-mediated responses to a bystander antigen (ovalbumin [ova]). maip-1 recognized only one of the three polypeptide chains of pas-1, but neutralized the suppressive effect of the whole worm extract on ova-specific antibody ... | 2006 | 16519731 |
| molecular evidence for the infection of zoo chimpanzees by pig ascaris. | we here describe the transmission of the pig roundworm, ascaris suum to chimpanzees maintained in the copenhagen zoo, denmark. using a technique for whole genome fingerprinting, amplified fragment length polymorphism (aflp) and the technique pcr restricted fragment length polymorphism (pcr-rflp) of the internal transcribed spacer region (its) of the nuclear ribosomal dna, the worms from the chimpanzees were compared with ascaris spp obtained from humans and pigs in order to identify the source o ... | 2006 | 16567044 |
| unique structure of ascaris suum b5-type cytochrome: an additional alpha-helix and positively charged residues on the surface domain interact with redox partners. | cytochrome b5 of the body wall of adult ascaris suum, a porcine parasitic nematode, is a soluble protein that lacks a c-terminal membrane-anchoring domain, but possesses an n-terminal pre-sequence of 30 amino acids. during the maturation of cytochrome b5, the n-terminal pre-sequence is proteolytically cleaved to form the mature protein of 82 amino acid residues. a. suum cytochrome b5 is a basic protein containing more lysine residues and exhibiting a higher midpoint redox potential than its mamm ... | 2006 | 16288599 |
| a recombinant enolase from anisakis simplex is differentially recognized in natural human and mouse experimental infections. | a 1,963-bp cdna was isolated from an anisakis simplex cdna library by immunoscreening with a hyperimmune rabbit serum raised against a crude extract of a. simplex l3 larvae. the open reading frame encodes a putative protein of 436 amino acid residues, which exhibits high similarity (70-80%) to enolase molecules from various other organisms, including helminth parasites. after subcloning and expression of the a. simplex cdna in pgex-4t-3, the resulting glutathione s-transferase fusion protein, pu ... | 2006 | 16049725 |
| gene expression and pharmacology of nematode nlp-12 neuropeptides. | this study examines the biology of nlp-12 neuropeptides in caenorhabditis elegans, and in the parasitic nematodes ascaris suum and trichostrongylus colubriformis. dyrplqfamide (1 nm-10 microm; n > or =6) produced contraction of innervated dorsal and ventral ascaris body wall muscle preparations (10 microm, 6.8+/-1.9 g; 1 microm, 4.6+/-1.8 g; 0.1 microm, 4.1+/-2.0 g; 10 nm, 3.8+/-2.0 g; n > or =6), and also caused a qualitatively similar, but quantitatively lower contractile response (10 microm, ... | 2006 | 16600246 |
| dermal microdialysis in the dog: in vivo assessment of the effect of cyclosporin a on cutaneous histamine and prostaglandin d2 release. | dermal microdialysis, a relatively noninvasive technique, allows investigation of the changes in cellular mediators released during cutaneous allergic responses. this technique was used to evaluate the effect of cyclosporin a, an immunosuppressive drug used for treatment of canine atopic dermatitis, on the cutaneous release of two pro-inflammatory mediators following intradermal allergen challenge. four beagle dogs spontaneously sensitized to ascaris suum were treated for 1 month with oral cyclo ... | 2006 | 16674731 |
| pulmonary lesions associated with visceral larva migrans due to ascaris suum or toxocara canis: imaging of six cases. | objective: the objective of our study was to evaluate chest radiographic and ct findings of patients with pulmonary lesions associated with visceral larva migrans due to ascaris suum or toxocara canis. ct investigation was focused on the location, size, contour, and internal features of the lesions; migration of lesions; mediastinal lymphadenopathy; and pleural effusion. conclusion: pulmonary visceral larva migrans appears on ct as multifocal subpleural nodules with halo or ground-glass opacitie ... | 2006 | 16714661 |
| prevalence of common gastrointestinal nematode parasites in scavenging pigs of different ages and sexes in eastern centre province, burkina faso. | the range and infestation intensities of gastrointestinal parasitic nematode species depend on the type of swine production system. the present study focused mainly on nematodes of veterinary importance in scavenging pigs in burkina faso, and aimed at determining the prevalence of gastro-intestinal nematode parasites by means of faecal egg per gram (epg) counts. between november 2001 and october 2002, faecal samples from 383 pigs of different sexes and ages (< 5 months, 5-12 months and > 12 mont ... | 2006 | 16715878 |
| isolation and identification of three new 5-alkenyl-3,3(2h)-furanones from two streptomyces species using a genomic screening approach. | analyses of biosynthetic gene clusters derived from streptomyces aculeolatus nrrl 18422 and streptomyces sp. eco86 indicated that both microorganisms have similar type i polyketide synthase (pks) gene clusters with relatively few genes encoding post-pks elaborative enzymes. however both gene clusters included a sequence coding for a relatively uncommon oxidative enzyme related to baeyer-villiger, flavin-type monooxygenases. screening of culture extracts for compounds with the predicted physicoch ... | 2006 | 16724457 |
| ascaris suum infections in pigs born and raised on contaminated paddocks. | the transmission of ascaris suum was studied in outdoor reared pigs. from may to june 2001, 6 farrowing paddocks were naturally contaminated with a. suum using experimentally infected seeder pigs. early july, 1 sow farrowed on each paddock. one piglet per litter was slaughtered every second week starting at week 3 post-partum (p.p.) for registration of liver white spots and recovery of a. suum from the lungs and the small intestine. the last pigs were slaughtered at week 19 p.p. faeces was exami ... | 2006 | 16740179 |
| regulation of anaphylactic igg1 antibody production by il-4 and il-10. | different cytokines have been implicated in the regulation of isotype expression in primary and secondary antibody responses. the aim of this study was to assess the regulation of anaphylactic igg1 and ige antibodies by il-4, il-10 and ifn-gamma at different time points of the antibody response against pi, an immunosuppressive fraction of ascaris suum extract, and ovalbumin (ova). | 2006 | 16804329 |
| mri studies of spinal visceral larva migrans syndrome. | we report serial mr findings in four patients with myelitis caused by visceral larva migrans syndrome due to toxocara canis or ascaris suum infection. mr imaging revealed spinal cord swelling with or without gadolinium enhancement in three patients. t2-weighted images showed high signal intensities preferentially located in both lateral and posterior columns. antihelmintic and corticosteroid treatment yielded improvement in neurologic deficits and spinal lesions. however, one patient with t. can ... | 2006 | 16815465 |
| whole-body periodic acceleration modifies experimental asthma in sheep. | nitric oxide is released from vascular endothelium in response to increased pulsatile shear stress. nitric oxide inhibits mast cell activation and is antiinflammatory and therefore might be protective in asthma. | 2006 | 16858016 |
| early stages of ascaris suum induce airway inflammation and hyperreactivity in a mouse model. | the inflammatory and functional changes that occur in murine lung after infection with 2500 infective ascaris suum eggs were studied in this work. a sequential influx of neutrophils, mononuclear cells and eosinophils occurred into airways concomitantly with migration of larvae from liver to the lungs. histological analysis of the lung showed a severe intra-alveolar haemorrhage at the peak of larval migration (day 8) and the most intense inflammatory cell infiltrate on day 14. ascaris l3 were fou ... | 2006 | 16916369 |
| fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of ascaris suum. | sodium fluoride (naf) is an anion that has been previously shown to block the moulting process of ascaris suum larvae. this study describes moulting and development-specific protein expression profiles of a. suum lung-stage l3 (asll3) following naf exposure. asll3s cultured in the presence or absence of naf were prepared for protein analysis using two-dimensional (2d) electrophoresis. naf exposure inhibited at least 22 proteins in asll3 compared with moulted larvae (i.e. asll4). a further compar ... | 2006 | 16959050 |
| ascaris suum-derived products suppress mucosal allergic inflammation in an interleukin-10-independent manner via interference with dendritic cell function. | we have previously demonstrated that protection from allergic inflammation by ascaris suum infection was characterized by a global increase in interleukin-10 (il-10) and the development of protective cd4(+)/cd25(+) t cells (l. schopf, s. luccioli, v. bundoc, p. justice, c. c. chan, b. j. wetzel, h. h. norris, j. f. urban, jr., and a. keane-myers, investig. ophthalmol. vis. sci. 46:2772-2780, 2005). here, we used a. suum pseudocoelomic fluid (pcf) in lieu of infection to define molecular mechanis ... | 2006 | 16966410 |
| effect of piperazine (diethylenediamine) on the moulting, proteome expression and pyrophosphatase activity of ascaris suum lung-stage larvae. | piperazine (diethylenediamine) is an anthelmintic widely used against animal and bird ascariasis. in this study, we show that treatment with piperazine blocks ascaris suum larval moulting and development processes and affects larval proteome expression profiles. a. suum lung-stage l3 (ll3) obtained from an infected rabbit's lungs were cultured in rpmi medium in the presence of increasing concentrations of piperazine sulfate (pzes). our results showed that pzes potently inhibited moulting of a. s ... | 2006 | 16987490 |
| a protein extension to shorten rna: elongated elongation factor-tu recognizes the d-arm of t-armless trnas in nematode mitochondria. | nematode mitochondria possess extremely truncated trnas. of 22 trnas, 20 lack the entire t-arm. the t-arm is necessary for the binding of canonical trnas and ef (elongation factor)-tu (thermo-unstable). the nematode mitochondrial translation system employs two different ef-tu factors named ef-tu1 and ef-tu2. our previous study showed that nematode caenorhabditis elegans ef-tu1 binds specifically to t-armless trna. c. elegans ef-tu1 has a 57-amino acid c-terminal extension that is absent from can ... | 2006 | 16859488 |
| [comparative studies on the difference between ascaris lumbricoides and ascaris suum]. | there has been continued controversy on the taxonomy of ascaris lumbricoides linnaeus, 1758 from humans and ascaris suum goeze, 1782 from pigs. this article reviews a range of comparative studies related to host susceptibility, morphology, karyotype, immunology and biochemistry, as well as molecular genetics in recent years. | 2006 | 16862915 |
| antibody responses to ascaris-derived proteins and glycolipids: the role of phosphorylcholine. | in addition to proteins, glycolipids can be targets of antibody responses and contribute to host-pathogen interaction. following the structural analysis of ascaris lumbricoides-derived glycolipids, the antibody responses of a group of children with no, light and heavy infections were analysed. the role of the phosphorylcholine moiety, present on ascaris glycoproteins and glycolipids, in antibody reactivity of these infected individuals was determined. children carrying heavy infections showed hi ... | 2006 | 16879308 |
| a real-time pcr method for quantifying viable ascaris eggs using the first internally transcribed spacer region of ribosomal dna. | worldwide, 1.4 billion people are infected with the intestinal worm ascaris lumbricoides. as a result, ascaris eggs are commonly found in wastewater and sludges. the current microscopy method for detecting viable ascaris eggs is time- and labor-intensive. the goal of this study was to develop a real-time quantitative pcr (qpcr) method to determine the levels of total and viable ascaris eggs in laboratory solutions using the first internally transcribed spacer (its-1) region of ribosomal dna (rdn ... | 2006 | 17056687 |
| pharmacology of n-, l-, and b-subtypes of nematode nachr resolved at the single-channel level in ascaris suum. | pharmacological experiments on ascaris suum have demonstrated the presence of three (n-, l-, and b-) subtypes of cholinergic receptor mediating contraction of body wall muscle in parasitic nematodes. in the present study, these ionotropic acetylcholine (ach) receptors (nachrs) were activated by levamisole and bephenium under patch-clamp conditions and competitively antagonized by paraherquamide and 2-desoxoparaherquamide. a number of recordings exhibited three separate current amplitude levels, ... | 2006 | 17056760 |
| elisa antibody determination in patients with anisakiosis or toxocariosis using affinity chromatography purified antigen. | one of the fundamental aspects of a parasitic infection diagnosis is the use of adequate antigens to develop specific and sensitive immunoassays. this fact is especially complicated in nematode infection cases because of the high cross-reactivity among different parasites in this group. we performed an evaluation of anisakis simplex antigens purified by affinity chromatography. we used sera from 38 patients diagnosed with anisakis sensitization and sera from 35 patients with clinical suspicion o ... | 2006 | 17063674 |
| immunosuppressive components of ascaris suum down-regulate expression of costimulatory molecules and function of antigen-presenting cells via an il-10-mediated mechanism. | high-molecular-weight components (pi) of ascaris suum suppress both cell-mediated and humoral responses against ovalbumin (ova) via an il-4/il-10-dependent mechanism. the aim of this work was to investigate the effect of pi on the ability of apc to activate t cells and the role of il-10 in this process. flow cytometry analyses of mhc class ii, cd80, cd86 and cd40 molecules on ln cells from mice immunized with ova or ova+pi showed that pi inhibits expression of these molecules on unfractionated c ... | 2006 | 17109466 |
| operon conservation and the evolution of trans-splicing in the phylum nematoda. | the nematode caenorhabditis elegans is unique among model animals in that many of its genes are cotranscribed as polycistronic pre-mrnas from operons. the mechanism by which these operonic transcripts are resolved into mature mrnas includes trans-splicing to a family of sl2-like spliced leader exons. sl2-like spliced leaders are distinct from sl1, the major spliced leader in c. elegans and other nematode species. we surveyed five additional nematode species, representing three of the five major ... | 2006 | 17121468 |
| [the influence of the metarhizium fungi on the embryonic development of ascaris suum]. | purpose: the present study focused on the effect of two fungal species metarhizium flavoviride and metarhizium anisopliae on the embryonic development and viability of ascaris suum larvae. results: microscope examination revealed that the presence of the mycelium inhibited the egg development, compared to control. the fungus-exposed eggs featured zygote vacuolisation, irregular blastomere cleavage, and morphological disturbances in the stages of blastula, gastrula, and larva. the embryopathies w ... | 2006 | 17131843 |
| paecilaminol, a new nadh-fumarate reductase inhibitor, produced by paecilomyces sp. fki-0550. | a new nadh-fumarate reductase inhibitor, paecilaminol, was isolated from the cultured broth of a fungus paecilomyces sp. fki-0550. it is an amino alcohol compound, the structure being established as 2-amino-14,16-dimethyl-3-octadecanol. paecilaminol exhibited an ic50 value of 5.1 microm against ascaris suum nadh-fumarate reductase. | 2006 | 17136891 |
| sensitization of naive beagles by intradermal injection of an ascaris antigen: induction of a model of skin allergy. | ascaris suum-hypersensitive beagles represent a unique model of skin allergy. despite its suitability, the need to test numerous dogs prior to the selection of natural positive responders causes many complications. we hypothesized that the model could be induced in adult beagles by primary sensitization. ten dogs were included in an intradermal sensitization program using ascaris antigen and were thereafter tested for cutaneous reaction. after 2 weeks, 60% developed a positive reaction that last ... | 2006 | 17190744 |
| [the time of development of ascarid eggs in the soil of the central chernozem zone (in case of the kursk region)]. | 2006 | 17290910 | |
| verticipyrone, a new nadh-fumarate reductase inhibitor, produced by verticillium sp. fki-1083. | a new nadh-fumarate reductase inhibitor, verticipyrone, was isolated from the cultured broth of a fungus, verticillium sp. fki-1083. the structure was established as (e)-2-methoxy-3,5-dimethyl-6-(3-methyl-2-undecenyl)-4h-pyran-4-one. verticipyrone exhibited an ic50 value of 0.88 nm against nadh-fumarate reductase of ascaris suum. verticipyrone inhibited both ascaris and bovine heart complex i, and its synthetic analogue, 8,9-dihydro-8-hydroxyverticipyrone, showed good selectivity against ascaris ... | 2006 | 17323645 |
| [limitation in the population of parasitic geohelminths by saprotrophic soil fungi and their secretions]. | the aim of the study was to examine the effect of mould fungi isolated from soil and of two of their metabolites, aflatoxin g1 and ochratoxin a, on the embryonic development of eggs and mortality of larvae of ascaris suum. an attempt was made to demonstrate synergism between fungi species in the action on embryogenesis of ascaris suum. | 2006 | 17385347 |
| modification of cepsilon mrna expression by ebv-encoded latent membrane protein 1. | to evaluate the effect of expression of latent membrane protein (lmp) 1 encoded by epstein-barr virus (ebv) on cepsilon mrna expression, mrna levels were examined by rt-pcr or northern blot analysis upon transient transfection of lmp1 in the splenocytes derived from brown-norway rats with or without immunization with 2,4-dinitrophenyl-conjugated ascaris suum antigen. splenocytes were transfected with lmp1 expression vector, psg5-lmp1, using lipofection method. cepsilon mrna levels were considera ... | 2006 | 17392589 |
| concurrent infection with trichinella spiralis and other helminths in pigs. | the aim of this study was to investigate possible influence of different helmintosis in the development of trichinella spiralis in experimental infected pigs. forty-two iberian pigs were allocated to six groups. three groups were single inoculated with ascaris suum, metastrongylus apri or t. spiralis, respectively. two groups were co-infected with t. spiralis and a. suum or t. spiralis and m. apri, respectively, while the last group included uninfected control pigs. clinical signs were only obse ... | 2007 | 17400390 |
| a comparison of the efficacy of two ivermectin formulations against larval and adult ascaris suum and oesophagostomum dentatum in experimentally infected pigs. | a study was conducted to evaluate and compare the efficacy of two injectable formulations of ivermectin (ivm-1 and ivm-2) at a dose rate of 0.3 mg/kg bodyweight versus placebo in the treatment and control of larval and adult stages of ascaris suum and oesophagostomum spp. in experimentally infected pigs. seventy helminth free pigs were allocated on a liveweight basis to 7 groups each comprising 10 pigs (a-g). group a served as an untreated control group. groups b and c were used to investigate t ... | 2007 | 17418952 |
| partial characterization of the antigen recognized by a monoclonal antibody to ascaris suum ovary extracts. | a monoclonal antibody produced against ovary extracts from the worm ascaris suum showed immunoreactivity against granules in the rachis and oocytes, the inner layer of the eggshell and the middle layer of some egg, but not against either ovary wall or uterus wall. furthermore, the same antigens were detected on the body surface of migrated larva in guinea pig lung, whereas none were detected in adult male worm or adult female worm, except for the female reproductive organs. the ovary extracts we ... | 2007 | 17391851 |
| infection with parasitic nematodes confounds vaccination efficacy. | t helper (th) cells produce signature cytokine patterns, induced largely by intracellular versus extracellular pathogens that provide the cellular and molecular basis for counter regulatory expression of protective immunity during concurrent infections. the production of il-12 and ifn-gamma, for example, resulting from exposure to many bacterial, viral, and protozoan pathogens is responsible for th1-derived protective responses that also can inhibit development of th2-cells expressing il-4-depen ... | 2007 | 17587500 |
| a comparison of modifications of the mcmaster method for the enumeration of ascaris suum eggs in pig faecal samples. | the comparative efficacies of seven published mcmaster method modifications for faecal egg counting were evaluated on pig faecal samples containing ascaris suum eggs. comparisons were made as to the number of samples found to be positive by each of the methods, the total egg counts per gram (epg) of faeces, the variations in epg obtained in the samples examined, and the ease of use of each of the methods. each method was evaluated after the examination of 30 samples of faeces. the positive sampl ... | 2007 | 17703889 |
| inhibitory receptor gp49b regulates eosinophil infiltration during allergic inflammation. | gp49b, an ig-like receptor, negatively regulates the activity of mast cells and neutrophils through cytoplasmic immunoreceptor tyrosine-based inhibition motifs. to characterize the role of gp49b further in vivo, gp49b-deficient mice were tested in two allergic models. responses to ragweed (rw) challenge in the lung and conjunctiva were assessed in models of allergic inflammation and during an infection with parasitic larvae of the nematode ascaris suum. infiltration by inflammatory cells into th ... | 2007 | 17761953 |
| inactivation of ascaris suum in a biodrying compost system. | pathogen contamination of waterways is a serious concern in dairy farming areas where livestock waste is applied to agricultural fields. as an alternative, a biodrying composting system dries collected livestock waste, reduces the strong odors, and has been proposed as a means of reducing, and even eliminating pathogens present in the waste. therefore, the survival of pathogens in a biodrying composting system was investigated. dairy farm livestock waste was piled in a biodrying storage shed whe ... | 2007 | 17766833 |
| parasite-specific il-4 responses in ascaris suum and trichuris suis-infected pigs evaluated by elispot. | the objective of the present study was to develop an elispot method to measure parasite-specific il-4 producing cells during experimental ascaris suum and trichuris suis infections in pigs. in many experimental settings it is useful to be able to measure changes in specifically induced cytokines over time at post-mrna level; in particular, specific measurement of il-4 is important for studies on nematodes due to the key function of il-4 in driving the th2 response. two separate experiments were ... | 2007 | 17883456 |
| [effects of some species of hydrocoles on the development of ascarid eggs]. | the study established that aquatic plants differently affected the development of ascarid eggs. some species of algae exerted a damaging effect on ascarid eggs only at early stages of embryogenesis while others did at its late stages, but some species did not produce any noticeable effect on the development of the eggs. a great difference was found in the development of ascarid eggs in different seasons. gas exchange conditions were ascertained to affect the development of ascarid eggs. analysis ... | 2007 | 17912827 |
| helminths of wild boar in the isolated population close to the northern border of its habitat area. | one hundred wild boars (sus scrofa) from a geographically isolated population on the island saaremaa of western estonia were examined for visceral helminths. seven helminth species, metastrongylus pudendotectus, m. salmi, m. elongatus, ascaris suum, trichuris suis, dicrocoelium dendriticum and taenia hydatigena larva, were found. the predominant helminths discovered were lung nematodes (prevalence 82%, mean intensity 96.2 per animal). a significant negative correlation was observed between the w ... | 2007 | 17964726 |
| western blot antibody determination in sera from patients diagnosed with anisakis sensitization with different antigenic fractions of anisakis simplex purified by affinity chromatography. | using western blot techniques, the specificities of crude and purified (pak and pas) anisakis simplex antigens were compared against 24 sera from patients diagnosed with anisakis sensitization. all patients recognized a 60 kda protein against the a. simplex crude extract, while 37.5% and 12.5% reacted with proteins of 40 and 25 kda, respectively, when igg was tested. in the case of ige determination, 41.6% of sera were negative, while 12.5% and 20.8% appeared to cross-react against toxocara cani ... | 2007 | 17974043 |
| analysis of binding interactions of pepsin inhibitor-3 to mammalian and malarial aspartic proteases. | the nematode ascaris suum primarily infects pigs, but also causes disease in humans. as part of its survival mechanism in the intestinal tract of the host, the worm produces a number of protease inhibitors, including pepsin inhibitor-3 (pi3), a 17 kda protein. recombinant pi3 expressed in e. coli has previously been shown to be a competitive inhibitor of a subgroup of aspartic proteinases: pepsin, gastricsin and cathepsin e. the previously determined crystal structure of the complex of pi3 with ... | 2007 | 18004881 |
| eosinophilic cholecystitis as a rare manifestation of visceral larva migrans. | eosinophilic cholecystitis is an infrequent form of cholecystitis. the etiology of eosinophilic cholecystitis is still obscure, and it is sometimes accompanied with several complications, but a simultaneous onset with pericarditis is very rare. we would like to make an alternative interpretation of our recent report "kaji k, yoshiji h, yoshikawa m, yamazaki m, ikenaka y, noguchi r, sawai m, ishikawa m, mashitani t, kitade m, kawaratani h, uemura m, yamao j, fujimoto m, mitoro a, toyohara m, yosh ... | 2007 | 18023114 |
| multiple roles of arginine 181 in binding and catalysis in the nad-malic enzyme from ascaris suum. | the nad-malic enzyme catalyzes the oxidative decarboxylation of l-malate. structures of the enzyme indicate that arginine 181 (r181) is within hydrogen bonding distance of the 1-carboxylate of malate in the active site of the enzyme and interacts with the carboxamide side chain of the nicotinamide ring of nadh, but not with nad+. data suggested r181 might play a central role in binding and catalysis in malic enzyme, and it was thus changed to lysine and glutamine to probe its potential function. ... | 2007 | 18027982 |
| decursin and decursinol angelate selectively inhibit nadh-fumarate reductase of ascaris suum. | nadh-fumarate reductase (nfrd) is a key enzyme in many anaerobic helminths. decursin and decursinol angelate have been isolated from the roots of angelica gigas nakai (apiaceae) as nfrd inhibitors. they inhibited ascaris suum nfrd with ic (50) values of 1.1 and 2.7 microm, respectively. their target is the electron transport enzyme complex i. since the inhibitory activities of decursin against bovine heart complexes are weak, it is a selective inhibitor of the nematode complex i. in contrast, de ... | 2007 | 17948169 |
| il-13 is a novel therapeutic target in allergic asthma. | evaluation of: bree a, schlerman fj, wadanoli m et al. il-13 blockade reduces lung inflammation after ascaris suum challenge in cynomolgus monkeys. j. allergy clin. immunol. 119, 1251-1257 (2007). there is abundant evidence supporting il-13 as having a central role in the pathology of allergic asthma. il-13 induces immunological responses relevant to allergic asthma and exerts effects on airway structural cells, leading to remodeling and enhanced contractility of airway smooth muscle. this paper ... | 2007 | 20477017 |
| a ser/thr kinase required for membrane-associated assembly of the major sperm protein motility apparatus in the amoeboid sperm of ascaris. | leading edge protrusion in the amoeboid sperm of ascaris suum is driven by the localized assembly of the major sperm protein (msp) cytoskeleton in the same way that actin assembly powers protrusion in other types of crawling cell. reconstitution of this process in vitro led to the identification of two accessory proteins required for msp polymerization: an integral membrane phosphoprotein, msp polymerization-organizing protein (mpop), and a cytosolic component, msp fiber protein 2 (mfp2). here, ... | 2007 | 17344482 |
| the effect of opiates and opiate antagonists on heat latency response in the parasitic nematode ascaris suum. | the effects of the opiates morphine and morphine-6-glucuronide (m6g), the mu opioid receptor specific antagonist d-phe-cys-tyr-d-trp-om-thr-pen-thr-nh(2) (ctop), and the general opiate antagonist naloxone on the latency of response to thermal stimulation were determined in the parasitic nematode ascaris suum. thermal detection and avoidance behaviors of the worms were evaluated with a tail flick analgesia meter using a modification of a technique employed for nociception experiments in rodents. ... | 2007 | 17363006 |
| il-13 blockade reduces lung inflammation after ascaris suum challenge in cynomolgus monkeys. | airway inflammation is a hallmark feature of asthma and a driver of airway hyperresponsiveness. il-13 is a key inducer of airway inflammation in rodent models of respiratory disease, but a role for il-13 has not been demonstrated in primates. | 2007 | 17379289 |
| migration behaviour and pathogenesis of five ascarid nematode species in the mongolian gerbil meriones unguiculatus. | to understand the characteristic features of the mongolian gerbil, meriones unguiculatus, as an animal model of ascarid infections, the migration behaviour and pathogenesis of larvae were investigated in experimentally infected gerbils. embryonated eggs from each of toxocara canis, baylisascaris procyonis, b. transfuga, ascaris suum, and a. lumbricoides were orally inoculated into gerbils and larvae were recovered from various organs at designated periods. in t. canis-infected gerbils, larvae we ... | 2007 | 17381866 |
| the migration of ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible c57bl/6j and resistant cba/ca mice. | ascariasis is an important infection in humans (ascaris lumbricoides) and pigs (ascaris suum) and individuals appear to be predisposed to either heavy or light worm burdens. these extremes of susceptibility and resistance are represented in a mouse model by 2 strains of mice, cba mice showing high resistance to infection and c57bl/6 which are highly susceptible, as reflected in worm burdens in the lungs 6-7 days after infection. in an attempt to identify the point at which the difference between ... | 2007 | 17381887 |
| functional, inflammatory and morphological characterisation of a cat model of allergic airway inflammation. | the aims of this study were to characterise a model of feline allergic airway inflammation and to test through a longitudinal investigation whether five repeated allergen exposures would lead to signs of airway remodelling that would be detectable in vivo. eight healthy control cats and eight cats sensitised with ascaris suum allergens were investigated. barometric whole body plethysmography (bwbp) was used for the assessment of respiratory variables and airway responsiveness (ar). bronchoalveol ... | 2007 | 17306576 |
| effects of age and allergen-induced airway inflammation in cats: radiographic and cytologic correlation. | thoracic radiography is an important diagnostic tool for feline respiratory medicine. the aim of this study was (1) to assess age-related changes of thoracic radiographic views in healthy young cats and (2) to test if experimentally-induced bronchial inflammation by inhaling ascaris suum (as) allergens leads to radiographic changes after single or repeated exposures. healthy cats (n=15-30) aged between 6 and 30 months were evaluated. eight healthy cats and eight as-sensitised cats, respectively, ... | 2007 | 17218130 |
| l-454,560, a potent and selective pde4 inhibitor with in vivo efficacy in animal models of asthma and cognition. | type 4 phosphodiesterases (pde4) inhibitors are emerging therapeutics in the treatment of a number of chronic disorders including asthma, chronic obstructive pulmonary disease (copd) and cognitive disorders. this study delineates the preclinical profile of l-454,560, which is a potent, competitive and preferential inhibitor of pde4a, 4b, and 4d with ic50 values of 1.6, 0.5 and 1.2 nm, respectively. in contrast to the exclusive binding of cilomilast and the preferential binding of roflumilast to ... | 2007 | 17428447 |
| peptide products of the afp-6 gene of the nematode ascaris suum have different biological actions. | matrix-assisted laser desorption/ionization time-of-flight and tandem time-of-flight (maldi-tof and maldi-tof/tof) mass spectrometry were used to sequence and localize three novel, related neuropeptides in the nervous system of the nematode ascaris suum, amrnalvrfamide (af21), ngapqpfvrfamide (af22), and sgmrnalvrfamide (af23). the amino acid sequences were used to clone a novel neuropeptide gene (afp-6) that encodes a precursor bearing a single copy of each of the peptides. in situ hybridizatio ... | 2007 | 17436302 |
| decrease of time for pathogen inactivation in alkaline disinfection systems using pressure. | from field studies conducted by tulane university (new orleans, louisiana), efficiency of advanced alkaline disinfection in closed systems was found to depend on ammonia concentration, ph, exposure time, temperature, total solids content, pretreatment storage time, and mixing effectiveness. in this study of a closed alkaline system, an additional pathogen stressor pressure was tested. the effect of the alkaline dosing has been assessed for dewatered raw and aerobically and anaerobically digested ... | 2007 | 17489273 |
| modulation of anaphylaxis by helminth-derived products in animal models. | helminths have a profound immunomodulatory effect upon the inductive and effector phases of inflammatory responses, including allergy. several animal models of anaphylaxis have been established to investigate the mechanisms by which helminth infections or helminth-derived products interfere with the onset of allergic reactions. the focus of our studies was the immunosuppression induced by the intestinal roundworm ascaris suum in the production of anaphylactic antibodies and the development of lu ... | 2007 | 17504662 |
| the nematode neuropeptide, af2 (kheylrf-nh2), increases voltage-activated calcium currents in ascaris suum muscle. | resistance to all the classes of anti-nematodal drugs like the benzimidazoles, cholinergic agonists and avermectins, has now been recorded in parasites of animals and/or humans. the development of novel anthelmintics is an urgent and imperative need. receptors of nematode neuropeptides have been suggested to be suitable target sites for novel anthelmintic drugs. | 2007 | 17519945 |
| pulmonary computed tomography findings of visceral larva migrans caused by ascaris suum. | to retrospectively evaluate the computed tomography (ct) findings of pulmonary involvement in patients with visceral larva migrans caused by ascaris suum. | 2007 | 17538287 |