Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genomic mutational analysis of the impact of the classical strain improvement program on β-lactam producing penicillium chrysogenum. | penicillium chrysogenum is a filamentous fungus that is employed as an industrial producer of β-lactams. the high β-lactam titers of current strains is the result of a classical strain improvement program (csi) starting with a wild-type like strain more than six decades ago. this involved extensive mutagenesis and strain selection for improved β-lactam titers and growth characteristics. however, the impact of the csi on the secondary metabolism in general remains unknown. | 2015 | 26572918 |
| enhanced production of polygalacturonase in solid-state fermentation: selection of the process conditions, isolation and partial characterization of the enzyme. | polygalacturonase (pg) production by penicillium chrysogenum during solid-state fermentation was accompanied by decomposition of orange peels. a leaching procedure was developed through the selection of solvent, time and intensity of stirring. a maximum pg activity was observed after 48 h peel inoculation. further cultivation decreased the enzyme activity significantly, up to 60% of the maximum pg activity. during fermentation, a rapid acidification of the solid medium which inhibited the pectin ... | 2015 | 26505127 |
| in vivo kinetic analysis of the penicillin biosynthesis pathway using paa stimulus response experiments. | in this study we combined experimentation with mathematical modeling to unravel the in vivo kinetic properties of the enzymes and transporters of the penicillin biosynthesis pathway in a high yielding penicillium chrysogenum strain. the experiment consisted of a step response experiment with the side chain precursor phenyl acetic acid (paa) in a glucose-limited chemostat. the metabolite data showed that in the absence of paa all penicillin pathway enzymes were expressed, leading to the productio ... | 2015 | 26476338 |
| sensitization rates for various allergens in children with allergic rhinitis in qingdao, china. | the aim of this study was to determine the prevalence of sensitization to common allergens in children with allergic rhinitis (ar) living in qingdao, china. we conducted a retrospective analysis for ar cases, who underwent skin prick tests (spt) in qingdao. a total of 2841 children with ar qualified for the inclusion criteria (age 3-5 years: 1500 children; age 6-12 years: 1341 children). the most common inhaled allergens to which the ar children were sensitive were dermatophagoides pteronyssinus ... | 2015 | 26371014 |
| enhanced production of α-amylase by penicillium chrysogenum in liquid culture by modifying the process parameters. | in this paper, we have assessed the role of changing physicochemical parameters and substrate types on the production of α-amylase enzyme from penicillium chrysogenum, with a view to determining the optimal conditions required for its maximum production. the findings of this research revealed that, at ph 6 using linseed oil cake as substratum, α-amylase enzyme production was maximum (550.0 u/ml), when the fungi was incubated for 6 days at 30 °c in 0.1 m acetate buffer. further, reasonably good p ... | 2015 | 26220910 |
| penicillium chrysogenum as a model system for studying cellular effects of methylglyoxal. | α-oxoaldehydes are formed as toxic by-products during metabolic activity. the biologically most important compound of this class, methylglyoxal, results from spontaneous phosphate elimination from dihydroxyacetone phosphate and glyceraldehyde 3-phosphate which are intermediate glycolysis products. methylglyoxal-mediated modification of lipids, nucleic acids and proteins is known to lead to the formation of advanced glycation end products. these modifications contribute to the aetiology of severe ... | 2015 | 26156309 |
| pcfkh1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in penicillium chrysogenum. | penicillin biosynthesis in penicillium chrysogenum (re-identified as penicillium rubens) is a good example of a biological process subjected to complex global regulatory networks and serves as a model to study fungal secondary metabolism. the winged-helix family of transcription factors recently described, which includes the forkhead type of proteins, is a key type of regulatory proteins involved in this process. in yeasts and humans, forkhead transcription factors are involved in different proc ... | 2015 | 26049046 |
| a natural short pathway synthesizes roquefortine c but not meleagrin in three different penicillium roqueforti strains. | the production of mycotoxins and other secondary metabolites in penicillium roqueforti is of great interest because of its long history of use in blue-veined cheese manufacture. in this article, we report the cloning and characterization of the roquefortine gene cluster in three different p. roqueforti strains isolated from blue cheese in the usa (the type strain), france, and the uk (cheshire cheese). all three strains showed an identical roquefortine gene cluster organization and almost identi ... | 2015 | 25998659 |
| sexual recombination as a tool for engineering industrial penicillium chrysogenum strains. | the recent discovery and functional characterization of opposite mating-type loci in the industrial penicillin producer penicillium chrysogenum demonstrated their regulatory role in sexual as well as asexual development. subsequent experiments further showed that a sexual life cycle can be induced in p. chrysogenum that was for long believed to reproduce exclusively by asexual propagation. finally, crossing of wild type and production strains resulted in the generation of recombinant ascospore i ... | 2015 | 25993917 |
| dicer-dependent biogenesis of small rnas and evidence for microrna-like rnas in the penicillin producing fungus penicillium chrysogenum. | micrornas (mirnas) are non-coding small rnas (srnas) that regulate gene expression in a wide range of eukaryotes. in this study, we analyzed regulatory srnas in penicillium chrysogenum, the industrial producer of the β-lactam antibiotic penicillin. to identify srnas and microrna-like rnas (milrnas) on a global approach, two srna sequencing libraries were constructed. one library was created with pooled total rna, obtained from twelve differently grown cultures (rna mix), and the other with total ... | 2015 | 25955857 |
| growth inhibition and stability of pgafp from penicillium chrysogenum against fungi common on dry-ripened meat products. | dry-ripened foods favor the development of a superficial fungal population that may include toxigenic molds. to combat unwanted molds, an antifungal protein from penicillium chrysogenum (pgafp) can be useful. the aim of the present work was to study the antimicrobial activity of pgafp against microorganisms common in dry-ripened foods, and to evaluate its sensitivity to proteolytic enzymes and heat treatments that may be applied to foods, as well as to different ph values. the inhibitory effect ... | 2015 | 25874751 |
| a mutasynthesis approach with a penicillium chrysogenum δroqa strain yields new roquefortine d analogues. | penicillium chrysogenum, which lacks the roqa gene, processes synthetic, exogenously added histidyltryptophanyldiketopiperazine (htd) to yield a set of roquefortine-based secondary metabolites also produced by the wild-type strain. feeding a number of synthetic htd analogues to the δroqa strain gives rise to the biosynthesis of a number of new roquefortine d derivatives, depending on the nature of the synthetic htd added. besides delivering semisynthetic roquefortine analogues, the mutasynthesis ... | 2015 | 25766600 |
| errata to "reminiscence of phospholipase b in penicillium notatum". | 2015 | 25743064 | |
| analysis of autophagy in penicillium chrysogenum by using starvation pads in combination with fluorescence microscopy. | the study of cellular quality control systems has emerged as a highly dynamic and relevant field of contemporary research. it has become clear that cells possess several lines of defense against damage to biologically relevant molecules like nucleic acids, lipids and proteins. in addition to organelle dynamics (fusion/fission/motility/inheritance) and tightly controlled protease activity, the degradation of surplus, damaged or compromised organelles by autophagy (cellular 'self-eating') has rece ... | 2015 | 25741895 |
| screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase. | the potency for production of cystathionine γ-lyase (cgl) by the fungal isolates was screened. among the tested twenty-two isolates, aspergillus carneus was the potent cgl producer (6.29 u/mg), followed by a. ochraceous (6.03 u/mg), a. versicolor (2.51 u/mg), a. candidus (2.12 u/mg), a. niveus and penicillium notatum (2.0 u/mg). the potent six isolates producing cgl was characterized morphologically, a. carneus kf723837 was further molecularly characterized based on the sequence of 18s-28s rdna. ... | 2015 | 25740443 |
| genome-wide identification of target genes of a mating-type α-domain transcription factor reveals functions beyond sexual development. | penicillium chrysogenum is the main industrial producer of the β-lactam antibiotic penicillin, the most commonly used drug in the treatment of bacterial infections. recently, a functional mat1-1 locus encoding the α-box transcription factor mat1-1-1 was discovered to control sexual development in p. chrysogenum. as only little was known from any organism about the regulatory functions mediated by mat1-1-1, we applied chromatin immunoprecipitation combined with next-generation sequencing (chip-se ... | 2015 | 25728030 |
| fungal morphology in industrial enzyme production--modelling and monitoring. | filamentous fungi are widely used in the biotechnology industry for the production of industrial enzymes. thus, considerable work has been done with the purpose of characterizing these processes. the ultimate goal of these efforts is to be able to control and predict fermentation performance on the basis of "standardized" measurements in terms of morphology, rheology, viscosity, mass transfer and productivity. however, because the variables are connected or dependent on each other, this task is ... | 2015 | 25724310 |
| penicitols a-c and penixanacid a from the mangrove-derived penicillium chrysogenum hdn11-24. | three new citrinin analogues, penicitols a-c (1-3), and one new xanthone derivative, penixanacid a (4), together with four known biogenetically related compounds (5-8), were discovered from the extract of a mangrove-derived fungus, penicillium chrysogenum hnd11-24. the structures of penicitols a-c and penixanacid a were established through analysis of extensive spectroscopic data. their cytotoxic activity against hela, bel-7402, hek-293, hct-116, and a549 cell lines was evaluated. | 2015 | 25611519 |
| pyomelanin production in penicillium chrysogenum is stimulated by l-tyrosine. | from a tomb in upper egypt we isolated a strain of penicillium chrysogenum that was capable of producing brown pigment in vitro when grown in a minimal salts medium containing tyrosine. we present evidence that this pigment is a pyomelanin, a compound that is known to assist in the survival of some micro-organisms in adverse environments. we tested type strains of pe. chrysogenum, which were also able to produce this pigment under similar conditions. inhibitors of the dhn and dopa melanin pathwa ... | 2015 | 25568259 |
| microarray hybridization analysis of light-dependent gene expression in penicillium chrysogenum identifies bzip transcription factor pcatfa. | the fungal velvet complex is a light-dependent master regulator of secondary metabolism and development in the major penicillin producer, penicillium chrysogenum. however, the light-dependent mechanism is unclear. to identify velvet-dependent transcriptional regulators that show light-regulated expression, we performed microarray hybridizations with rna isolated from p. chrysogenum δpcku70 cultures grown under 13 different long-term, light-dependent growth conditions. we compared these expressio ... | 2015 | 25557366 |
| a kinetic study of textile dyeing wastewater degradation by penicillium chrysogenum. | the potential of penicillium chrysogenum to decolorize azo dyes and a real industrial textile wastewater was studied. p. chrysogenum was able to decolorize and degrade three azo dyes (200 mg l(-1)), either independently or in a mixture of them, using glucose as a carbon source. a kinetic model for degradation was developed and it allowed predicting the degradation kinetics of the mixture of the three azo dyes. in addition, p. chrysogenum was able to decolorize real industrial wastewater. the kin ... | 2015 | 25555702 |
| transformation of waste cooking oil into c-18 fatty acids using a novel lipase produced by penicillium chrysogenum through solid state fermentation. | the prime aim of the current work was to illustrate the components existing in repeatedly used cooking oil and to develop an economical process for the production of fatty acids from low cost feedstock waste. the waste cooking oil was characterized by the occurrence of high molecular weight hydrocarbons and polymerized derivative of esters. triacontanoic acid methyl ester, 2,3,5,8-tetramethyldecane, 3,3 dimethyl heptane, and 2,2,3,3-teramethyl pentane were detected as thermal and oxidative conta ... | 2015 | 28324521 |
| a mat1-2 wild-type strain from penicillium chrysogenum: functional mating-type locus characterization, genome sequencing and mating with an industrial penicillin-producing strain. | in heterothallic ascomycetes, mating is controlled by two nonallelic idiomorphs that determine the 'sex' of the corresponding strains. we recently discovered mating-type loci and a sexual life cycle in the penicillin-producing fungus, penicillium chrysogenum. all industrial penicillin production strains worldwide are derived from a mat1-1 isolate. no mat1-2 strain has been investigated in detail until now. here, we provide the first functional analysis of a mat1-2 locus from a wild-type strain. ... | 2015 | 25521009 |
| microwave assisted one-pot catalyst free green synthesis of new methyl-7-amino-4-oxo-5-phenyl-2-thioxo-2,3,4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates as potent in vitro antibacterial and antifungal activity. | an efficiently simple protocol for the synthesis of methyl 7 amino-4-oxo-5-phenyl-2-thioxo-2, 3, 4,5-tetrahydro-1h-pyrano[2,3-d]pyrimidine-6-carboxylates via one-pot three component condensation pathway is established via microwave irradiation using varied benzaldehyde derivatives, methylcyanoacetate and thio-barbituric acid in water as a green solvent. a variety of functionalized substrates were found to react under this methodology due to its easy operability and offers several advantages like ... | 2015 | 26644932 |
| proteomic analysis of anti-cancerous scopularide production by a marine microascus brevicaulis strain and its uv mutant. | the marine fungus microascus brevicaulis strain lf580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides a and b, which exhibit distinct activities against tumour cell lines. a mutant strain was obtained using uv mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. here, we show the first proteome study of a marine fungus. comparative proteomics were applied to gain deeper understanding o ... | 2015 | 26460745 |
| 2,4-di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated lactococcus sp. | the volatile organic compound 2,4-di-tert-butyl phenol (2,4 dtbp) was purified from the cell free supernatant of a newly isolated lactococcus sp. by solvent extraction and chromatographic techniques. molecular characterization of the compound by esi-ms, (1)h nmr and ftir analysis revealed the structure, c14h22o. fungicidal activity was demonstrated against aspergillus niger, fusarium oxysporum and penicillium chrysogenum by disc diffusion assay. among the cell lines tested for cytotoxicity of th ... | 2015 | 26164257 |
| effects of three volatile oxylipins on colony development in two species of fungi and on drosophila larval metamorphosis. | the aim of this study is to investigate the effects of three volatile oxylipins on colony development in two fungi and on drosophila larval metamorphosis. using an airborne exposure technique, three common and volatile oxylipins (1-octen-3-ol, (e)-2-hexenal, and 1-hexanol) were compared for their effects on spore germination and colony growth in aspergillus niger and penicillium chrysogenum, as well as for their effects on the morphogenesis of larvae of drosophila melanogaster. conidia of both a ... | 2015 | 26126831 |
| environmental mold and mycotoxin exposures elicit specific cytokine and chemokine responses. | molds can cause respiratory symptoms and asthma. we sought to use isolated peripheral blood mononuclear cells (pbmcs) to understand changes in cytokine and chemokine levels in response to mold and mycotoxin exposures and to link these levels with respiratory symptoms in humans. we did this by utilizing an ex vivo assay approach to differentiate mold-exposed patients and unexposed controls. while circulating plasma chemokine and cytokine levels from these two groups might be similar, we hypothesi ... | 2015 | 26010737 |
| synthesis, spectral, thermal, fluorescence, antimicrobial, anthelmintic and dna cleavage studies of mononuclear metal chelates of bi-dentate 2h-chromene-2-one schiff base. | the co(ii), ni(ii) and cu(ii) complexes have been synthesized with schiff base (hl), derived from 8-formyl-7-hydroxy-4-methylcoumarin with benzylamine. the schiff base and its metal complexes were structurally characterized based on ir, (1)h nmr, (13)c nmr, uv-visible, esr, magnetic, thermal, fluorescence, mass and esi-ms studies. the complexes are completely soluble in dmf and dmso. the molar conductance values indicate that, all synthesized metal complexes are non-electrolytic in nature. eleme ... | 2015 | 26002539 |
| protein kinase a signaling and calcium ions are major players in paf mediated toxicity against aspergillus niger. | the penicillium chrysogenum antifungal protein paf is toxic against potentially pathogenic ascomycetes. we used the highly sensitive aequorin-expressing model aspergillus niger to identify a defined change in cytoplasmic free ca(2+) dynamics in response to paf. this ca(2+) signature depended on an intact positively charged lysine-rich paf motif. by combining ca(2+) measurements in a. niger mutants with deregulated camp/protein kinase a (pka) signaling, we proved the interconnection of ca(2+) per ... | 2015 | 25882631 |
| untargeted metabolic profiling of vitis vinifera during fungal degradation. | this paper illustrates the application of an untargeted metabolic profiling analysis of winery-derived biomass degraded using four filamentous fungi (trichoderma harzianum, aspergillus niger, penicillium chrysogenum and p. citrinum) and a yeast (saccharomyces cerevisiae). analysis of the metabolome resulted in the identification of 233 significant peak features [p < 0.05; fold change (fc) > 2 and signal-to-noise ratio >50] using gas chromatography-mass spectrometry followed by statistical chemom ... | 2015 | 25868913 |
| synthesis, characteristics and antimicrobial activity of zno nanoparticles. | the utilization of various plant resources for the bio synthesis of metallic nano particles is called green technology and it does not utilize any harmful protocols. present study focuses on the green synthesis of zno nano particles by zinc carbonate and utilizing the bio-components of powder extract of dry ginger rhizome (zingiber officinale). the zno nano crystallites of average size range of 23-26 nm have been synthesized by rapid, simple and eco friendly method. zinc oxide nano particles wer ... | 2015 | 25748589 |
| synthesis, spectral investigation (¹h, ¹³c) and anti-microbial screening of benzophenone imines. | new series of benzophenone imines with general formula ph2-c=nr; r = benzyl, 4-fluorobenzyl, naphthyl, phenyl, 4-nitrophenyl were synthesized by condensation of dichlorodiphenylmethane and different aromatic primary amines (1:1) those imines were characterized by different physiochemical and spectroscopic techniques like melting point, elemental analysis, ft-ir, multinuclear nmr (¹h, ¹³c). after characterization, imines were subjected to anti-microbial activities. all compounds showed promising ... | 2015 | 26639485 |
| identification and characterization of three penicillium chrysogenum α-l-arabinofuranosidases (pcabf43b, pcabf51c, and afq1) with different specificities toward arabino-oligosaccharides. | we previously described four α-l-arabinofuranosidases (abfs) secreted by penicillium chrysogenum 31b. here, we cloned the fifth and sixth genes (pcabf43b and pcabf51c) encoding the abfs pcabf43b and pcabf51c in this strain and overexpressed these genes in escherichia coli. the deduced amino acid sequences of pcabf43b and pcabf51c were highly similar to putative abfs belonging to glycoside hydrolase families 43 and 51, respectively. semiquantitative reverse transcription polymerase chain reaction ... | 2015 | 26002506 |
| biochemical characterization and overexpression of an endo-rhamnogalacturonan lyase from penicillium chrysogenum. | rhamnogalacturonan lyase (pcrgl4a) was purified from the culture supernatant of penicillium chrysogenum 31b. pcrgl4a optimal activity occurred between ph 7-8 and at 40 °c. conserved domain search analysis identified pcrgl4a as a member of polysaccharide lyase family 4. pcrgl4a contains two conserved catalytic and four conserved substrate-binding residues as determined by x-ray crystallography of the aspergillus aculeatus rg lyase. recombinant pcrgl4a (rpcrgl4a) expressed in escherichia coli demo ... | 2015 | 25666014 |
| stereoselective bioreduction of α-azido ketones by whole cells of marine-derived fungi. | seven strains of marine-derived fungi (aspergillus sclerotiorum cbmai 849, cladosporium cladosporioides cbmai 857, penicillium raistrickii cbmai 931, penicillium citrinum cbma 1186, mucor racemosus cbmai 847, beauveria felina cbmai 738, and penicillium oxalicum cbmai 1185) and terrestrial fungus penicillium chrysogenum cbma1199 were screened as catalysts for the asymmetric reduction of α-keto azides 5-8 to their corresponding β-azidophenylethanols 9-12. the marine fungi showed prelog and anti-pr ... | 2015 | 26272428 |
| determination of lipolytic and proteolytic activities of mycoflora isolated from dry-cured teruel ham. | fungi play a key role in dry-cured ham production because of their lipolytic and proteolytic activities. in the present study, 74 fungal strains from dry-cured teruel hams and air chambers were tested for proteolytic and lipolytic activities, with a view to their possible use as starter cultures. lipolytic activity of fungi was studied against lauric, palmitic, stearic and oleic acids, whereas proteolytic activity was studied against casein and myosin. of the 74 fungal strains tested, most of th ... | 2015 | 26243949 |
| molecular characterization of a penicillium chrysogenum exo-rhamnogalacturonan lyase that is structurally distinct from other polysaccharide lyase family proteins. | we previously described an endo-acting rhamnogalacturonan (rg) lyase, termed pcrgl4a, of penicillium chrysogenum 31b. here, we describe a second rg lyase, called pcrglx. we determined the cdna sequence of the pcrglx gene, which encodes pcrglx. based on analyses using a blast search and a conserved domain search, pcrglx was found to be structurally distinct from known rg lyases and might belong to a new polysaccharide lyase family together with uncharacterized fungal proteins of nectria haematoco ... | 2015 | 25921806 |
| fungi in ontario maple syrup & some factors that determine the presence of mold damage. | maple syrup is a high value artisanal product produced mainly in canada and a number of states primarily in the northeast usa. mold growth (wallemia sebi) on commercial product was first reported in syrup in 1908. since then, few data have been published. we conducted a systematic examination for fungi in maple syrup from 68 producers from all of the syrup-producing areas of ontario, canada. the mean ph of the samples was ph 6.82, sugar content averaged 68.0±0.89 °brix and aw averaged 0.841±0.01 ... | 2015 | 26001061 |
| diversity and bioprospection of fungal community present in oligotrophic soil of continental antarctica. | we surveyed the diversity and capability of producing bioactive compounds from a cultivable fungal community isolated from oligotrophic soil of continental antarctica. a total of 115 fungal isolates were obtained and identified in 11 taxa of aspergillus, debaryomyces, cladosporium, pseudogymnoascus, penicillium and hypocreales. the fungal community showed low diversity and richness, and high dominance indices. the extracts of aspergillus sydowii, penicillium allii-sativi, penicillium brevicompac ... | 2015 | 25809294 |
| the mycobiota of air inside and outside the meju fermentation room and the origin of meju fungi. | the fungi on meju are known to play an important role as degrader of macromolecule of soybeans. in order to elucidate the origin of fungi on traditional meju, mycobiota of the air both inside and outside traditional meju fermentation rooms was examined. from 11 samples of air collected from inside and outside of 7 meju fermentation rooms, 37 genera and 90 species of fungi were identified. in outside air of the fermentation room, cladosporium sp. and cladosporium cladosporioides were the dominant ... | 2015 | 26539041 |
| probiotic potential of lactobacillus strains with antifungal activity isolated from animal manure. | the aim of the study was to isolate and characterize the lactic acid bacteria (lab) from animal manure. among the thirty lab strains, four strains, namely, kcc-25, kcc-26, kcc-27, and kcc-28, showed good cell growth and antifungal activity and were selected for further characterization. biochemical and physiology properties of strains confirmed that the strains are related to the lactobacillus sp.; further, the 16s rrna sequencing confirmed 99.99% sequence similarity towards lactobacillus planta ... | 2015 | 26167534 |
| an evaluation of antifungal agents for the treatment of fungal contamination in indoor air environments. | fungal contamination in indoor environments has been associated with adverse health effects for the inhabitants. remediation of fungal contamination requires removal of the fungi present and modifying the indoor environment to become less favourable to growth. this may include treatment of indoor environments with an antifungal agent to prevent future growth. however there are limited published data or advice on chemical agents suitable for indoor fungal remediation. the aim of this study was t ... | 2015 | 26042369 |
| in-vitro assessment of the probiotic potential of lactobacillus plantarum kcc-24 isolated from italian rye-grass (lolium multiflorum) forage. | the aim of the present study was to determine the probiotic potential of the lactic acid bacteria lactobacillus plantarum kcc-24 (l. plantarum kcc-24), that was isolated and characterized from italian ryegrass (lolium multiflorum) forage. the following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low ph, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydropho ... | 2015 | 25576238 |
| insight into the antifungal mechanism of neosartorya fischeri antifungal protein. | small, cysteine-rich, highly stable antifungal proteins secreted by filamentous ascomycetes have great potential for the development of novel antifungal strategies. however, their practical application is still limited due to their not fully clarified mode of action. the aim of this work was to provide a deep insight into the antifungal mechanism of neosartorya fischeri antifungal protein (nfap), a novel representative of this protein group. within a short exposure time to nfap, reduced cellular ... | 2015 | 25994413 |
| lactic acid bacteria as functional probiotic isolates for inhibiting the growth of aspergillus flavus, a. parasiticus, a. niger and penicillium chrysogenum. | the aim of this study was to assess the potential of lactic acid bacteria (lab) such as lactobacillus acidophilus, l. rhamnosus, l. casei, l. paracasei and bifidobacterium bifidum to inhibit the outgrowth of some common food-spoiling fungi including aspergillus niger, a. flavus, a. parasiticus and penicillium chrysogenum. | 2015 | 26597145 |
| the effect of anatase and rutile crystallites isolated from titania p25 photocatalyst on growth of selected mould fungi. | antifungal properties of anatase and rutile crystallites isolated from commercial titania p25 photocatalyst were investigated by mycelium growth in the dark and under indoor light. investigated fungi, i.e., pseudallescheria boydii, scedosporium apiospermum, pseudallescheria ellipsoidea, scedosporium aurantiacum, aspergillus versicolor, aspergillus flavus, stachybotrys chartarum, penicillium chrysogenum, aspergillus melleus, were isolated from air and from moisture condensed on walls. anatase and ... | 2015 | 26177513 |
| impact of the antifungal protein pgafp from penicillium chrysogenum on the protein profile in aspergillus flavus. | antifungal proteins produced by molds are generally small, highly basic, and cysteine-rich. the best known effects of these proteins include morphological changes, metabolic inactivation, and membrane perturbation on sensitive fungi. reactive oxygen species (ros) generation leads to apoptosis, with g -protein playing a key role in transduction of cell death signals. the antifungal protein pgafp from penicillium chrysogenum inhibits growth of some toxigenic molds. here we analyzed the effect of t ... | 2015 | 26078108 |
| control of household mycoflora in fermented sausages using phenolic fractions from olive mill wastewaters. | biopreservation using polyphenols represents an alternative to chemical molecules for improving food safety. in this work, we evaluated the antifungal activity of polyphenols extracted from olive mill wastewater (omwwp) to reduce or eliminate the growth of undesired fungi on the surface of dry fermented sausages. antagonism against penicillium expansum dsmz 1282, penicillium verrucosum dsmz 12639, penicillium nalgiovense ms01, aspergillus ochraceus dsmz 63304, cladosporium cladosporioides ms12, ... | 2015 | 25996624 |
| single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of penicillium chrysogenum. | the cholesterol-lowering blockbuster drug pravastatin can be produced by stereoselective hydroxylation of the natural product compactin. we report here the metabolic reprogramming of the antibiotics producer penicillium chrysogenum toward an industrial pravastatin production process. following the successful introduction of the compactin pathway into the β-lactam-negative p. chrysogenum ds50662, a new cytochrome p450 (p450 or cyp) from amycolatopsis orientalis (cyp105as1) was isolated to catalyz ... | 2015 | 25691737 |
| influence of the silver nanoparticles on microbial community in different environments. | the aim of this study was to assess the influence of silver nanoparticles (agnps) on the number and diversity of microbial community in different environments (soil extract, water, sewage), and to determine whether the environment inhibits or accelerates the influence of agnps on moulds. agnps (45 ppm) present in the environment decreased bacterial (91%) and fungal (33-85%) numbers, and eliminated some strains, e.g., alternaria alternata and cryptococcus laurentii. based on the biomass growth of ... | 2015 | 26610310 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| common airborne fungi induce species-specific effects on upper airway inflammatory and remodelling responses. | whilst the exact cause of chronic rhinosinusitis (crs) remains elusive, it is clear that both inflammation and remodelling are key disease processes. environmental fungi have been linked to airway inflammation in crs; however, their role in the pathogenesis of this condition remains controversial. the current consensus suggests that whilst fungi may not be directly causative, it is likely that crs patients have deficits in their innate and potentially acquired immunity, which in turn may modify ... | 2016 | 26501134 |
| specific antibodies for the detection of alternaria allergens and the identification of cross-reactive antigens in other fungi. | the mould alternaria alternata is an important source of respiratory allergens. a. alternata extracts show great variations regarding allergenic potency. the aim of this study was to generate antibody probes specific for important alternaria allergens and to use them to study allergen expression, depending on different culture conditions, as well as to search for cross-reactive allergens in other mould species. | 2016 | 27780168 |
| how to diagnose mould allergy? comparison of skin prick tests with specific ige results. | diagnosis of mould allergy is complicated due to the heterogeneity of the test material and the decrease in the number of commercial mould skin test solutions that are currently available. | 2016 | 27027397 |
| penicillium chrysogenum dsoa associated with marine sponge (tedania anhelans) exhibit antimycobacterial activity. | a strain of penicillium chrysogenum was isolated from tedania anhelans (marine sponge) collected from indian ocean (8°22'30″n latitude and 76°59'16″ longitude) and deposited in culture collection centers. the strain subjected to different culture conditions for production of extrolites were extracted using ethyl acetate and chloroform. when both extracts were subjected for antibacterial activity, latter had high activity. minimum inhibitory concentration of chloroform extract ranged from 31.25-1 ... | 2016 | 26717859 |
| evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards. | the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ... | 2016 | 26896133 |
| a natural anopheles-associated penicillium chrysogenum enhances mosquito susceptibility to plasmodium infection. | whereas studies have extensively examined the ability of bacteria to influence plasmodium infection in the mosquito, the tripartite interactions between non-entomopathogenic fungi, mosquitoes, and plasmodium parasites remain largely uncharacterized. here we report the isolation of a common mosquito-associated ascomycete fungus, penicillium chrysogenum, from the midgut of field-caught anopheles mosquitoes. although the presence of pe. chrysogenum in the anopheles gambiae midgut does not affect mo ... | 2016 | 27678168 |
| antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles. | biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix. | 2016 | 27400968 |
| a penicillium chrysogenum-based expression system for the production of small, cysteine-rich antifungal proteins for structural and functional analyses. | small, cysteine-rich and cationic antifungal proteins (aps) from filamentous ascomycetes, such as nfap from neosartorya fischeri and paf from penicillium chrysogenum, are promising candidates for novel drug development. a prerequisite for their application is a detailed knowledge about their structure-function relation and mode of action, which would allow protein modelling to enhance their toxicity and specificity. technologies for structure analyses, such as electronic circular dichroism (ecd) ... | 2016 | 27835989 |
| in vitro screening of mucus and solvent extracts of eisenia foetida against human bacterial and fungal pathogens. | earthworms are macro invertebrate and have been widely used as therapeutic drugs for thousands of years. in the current research, experiments viz., the antibacterial, antifungal and antioxidant activity of mucus and solvent extracts of eisenia foetida were conducted to investigate for the first time in pakistan against human infectious pathogens. antimicrobial activity of e. foetida against human pathogens underwent investigation through an agar disc diffusion method while an abts(•+) free radic ... | 2016 | 27166541 |
| role of nutrients and illuminance in predicting the fate of fungal mediated petroleum hydrocarbon degradation and biomass production. | biodegradation and biomass production are affected by numerous environmental factors including ph, oxygen availability and presence of pollutants. the present study, for the first time, elucidated the effects of nutrients and light on mycodegradation of petroleum hydrocarbons in diesel oil. seven fungal strains (aspergillus terreus fa3, aspergillus niger fa5, aspergillus terreus fa6, penicillium chrysogenum fp4, aspergillus terreus fp6, aspergillus flavus fp10, and candida sp. fg1) were used for ... | 2016 | 27039364 |
| occurrence of moulds from bee pollen in central italy--a preliminary study. | the present study aimed to preliminary evaluate the occurrence of fungi in 40 specimens of trapped pollen collected from april-september 2015 in 40 apiaries from tuscany (central italy). cultural and microscopical examinations allowed the recognition of cladosporium sp., alternaria sp., humicola sp. mucoraceae and acremonium sp. penicillium brevicompactum, penicillium chrysogenum, aspergillus flavus , aspergillus nidulans, aspergillus niger and aspergillus terreus were also identified. yeasts an ... | 2016 | 27010410 |
| evidence for synergistic activity of plant-derived essential oils against fungal pathogens of food. | the antifungal activities of eight essential oils (eos) namely basil, cinnamon, eucalyptus, mandarin, oregano, peppermint, tea tree and thyme were evaluated for their ability to inhibit growth of aspergillus niger, aspergillus flavus, aspergillus parasiticus and penicillium chrysogenum. the antifungal activity of the eos was assessed by the minimum inhibitory concentration (mic) using 96-well microplate analysis. the interactions between different eo combinations were done by the checkerboard te ... | 2016 | 26678126 |
| application of a low molecular weight antifungal protein from penicillium chrysogenum (paf) to treat pulmonary aspergillosis in mice. | paf, a small antifungal protein from penicillium chrysogenum, inhibits the growth of several pathogenic filamentous fungi, including members of the aspergillus genus. paf has been proven to have no toxic effects in vivo in mice by intranasal application. to test its efficacy against invasive pulmonary aspergillosis (ipa), experiments were carried out in mice suffering from ipa. adult mice were immunosuppressed and then infected with aspergillus fumigatus. after stable infection, the animals were ... | 2016 | 27826143 |
| efficacy of aqueous and methanolic extracts of rheum spiciformis against pathogenic bacterial and fungal strains. | rheum spiciformis is a newly identified edible medicinal plant of genus rheum. the plant is used to treat various diseases on traditional levels in kashmir valley, india. | 2016 | 27790424 |
| biodegradative activities of selected environmental fungi on a polyester polyurethane varnish and polyether polyurethane foams. | polyurethane (pu) is widely used in many aspects of modern life because of its versatility and resistance. however, pu waste disposal generates large problems, since it is slowly degraded, there are limited recycling processes, and its destruction may generate toxic compounds. in this work, we isolated fungal strains able to grow in mineral medium with a polyester pu (ps-pu; impranil dln) or a polyether pu (pe-pu; poly lack) varnish as the only carbon source. of the eight best impranil-degrading ... | 2016 | 27316963 |
| d-galactose catabolism in penicillium chrysogenum: expression analysis of the structural genes of the leloir pathway. | in this study, we analyzed the expression of the structural genes encoding the five enzymes comprising the leloir pathway of d-galactose catabolism in the industrial cell factory penicillium chrysogenum on various carbon sources. the genome of p. chrysogenum contains a putative galactokinase gene at the annotated locus pc13g10140, the product of which shows strong structural similarity to yeast galactokinase that was expressed on lactose and d-galactose only. the expression profile of the galact ... | 2016 | 27630054 |
| key role of laea and velvet complex proteins on expression of β-lactam and pr-toxin genes in penicillium chrysogenum: cross-talk regulation of secondary metabolite pathways. | penicillium chrysogenum is an excellent model fungus to study the molecular mechanisms of control of expression of secondary metabolite genes. a key global regulator of the biosynthesis of secondary metabolites is the laea protein that interacts with other components of the velvet complex (vela, velb, velc, vosa). these components interact with laea and regulate expression of penicillin and pr-toxin biosynthetic genes in p. chrysogenum. both laea and vela are positive regulators of the penicilli ... | 2016 | 27565675 |
| generation and characterization of indoor fungal aerosols for inhalation studies. | in the indoor environment, people are exposed to several fungal species. evident dampness is associated with increased respiratory symptoms. to examine the immune responses associated with fungal exposure, mice are often exposed to a single species grown on an agar medium. the aim of this study was to develop an inhalation exposure system to be able to examine responses in mice exposed to mixed fungal species aerosolized from fungus-infested building materials. indoor airborne fungi were sampled ... | 2016 | 26921421 |
| cytotoxic and antibiotic cyclic pentapeptide from an endophytic aspergillus tamarii of ficus carica. | a new cyclic pentapeptide, disulfide cyclo-(leu-val-ile-cys-cys) (1), named malformin e, together with 13 known cyclic dipeptides, was isolated from the culture broth of endophytic fungus fr02 from the roots of ficus carica. the strain fr02 was identified as aspergillus tamarii on the basis of morphological characteristics and molecular analyses of internal transcribed spacer (its). their structures were determined by the combination of 1d and 2d nmr spectroscopy, hrms (esi), uv, and marfey's an ... | 2016 | 27147299 |
| the capability of fungi isolated from moldy dwellings to produce toxins. | the main objective was analysis and assessment of toxinogenic capabilities of fungi isolated from moldy surfaces in residential rooms in an urban agglomeration situated far from flooded areas in moderate climate zone. | 2016 | 27518891 |
| molecular characterization and analysis of antimicrobial activity of endophytic fungi from medicinal plants in saudi arabia. | endophytic fungi, which have been reported in numerous plant species, are important components of the forest community and contribute significantly to the diversity of natural ecosystems. | 2016 | 27099679 |
| quantification and identification of microorganisms found on shell and kernel of fresh edible chestnuts in michigan. | chestnut is a relatively new cultivated crop for michigan, and postharvest loss due to decay has been problematic as production has increased each year. in 2007, more than 25% of chestnuts were lost to postharvest decay, equivalent to approximately 5300 kg of fresh product. to determine the organisms responsible for decay, a microbiological survey was performed in 2006 and 2007 to identify microorganisms involved in postharvest shell (external surface) mold and internal kernel (edible portion) d ... | 2016 | 26869338 |
| identification and control of moulds responsible for black spot spoilage in dry-cured ham. | the aims of this work were to identify moulds responsible for black spot spoilage in the drying and cellar stages of dry-cured ham processing and evaluate the effectiveness of preventive actions for controlling this alteration. four mould strains isolated from spoiled hams were identified by morphological characteristics and the its and β-tubulin sequencing. two of them were cladosporium oxysporum, one was c. cladosporioides and the remaining one was c. herbarum. these spoiling strains reproduce ... | 2016 | 27468139 |
| augmentation of a microbial consortium for enhanced polylactide (pla) degradation. | bioplastics are eco-friendly and derived from renewable biomass sources. innovation in recycling methods will tackle some of the critical issues facing the acceptance of bioplastics. polylactic acid (pla) is the commonly used and well-studied bioplastic that is presumed to be biodegradable. considering their demand and use in near future, exploration for microbes capable of bioplastic degradation has high potential. four pla degrading strains were isolated and identified as penicillium chrysogen ... | 2016 | 26843697 |
| fungi associated with rocks of the atacama desert: taxonomy, distribution, diversity, ecology and bioprospection for bioactive compounds. | this study assessed the diversity of cultivable rock-associated fungi from atacama desert. a total of 81 fungal isolates obtained were identified as 29 ascomycota taxa by sequencing different regions of dna. cladosporium halotolerans, penicillium chrysogenum and penicillium cf. citrinum were the most frequent species, which occur at least in four different altitudes. the diversity and similarity indices ranged in the fungal communities across the latitudinal gradient. the fisher-α index displaye ... | 2016 | 26235221 |
| production of 8,11-dihydroxy and 8-hydroxy unsaturated fatty acids from unsaturated fatty acids by recombinant escherichia coli expressing 8,11-linoleate diol synthase from penicillium chrysogenum. | hydroxy unsaturated fatty acids can be used as antimicrobial surfactants. 8,11-linoleate diol synthase (8,11-lds) catalyzes the conversion of unsaturated fatty acid to 8-hydroperoxy unsaturated fatty acid, and it is subsequently isomerized to 8,11-dihydroxy unsaturated fatty acid by the enzyme. the optimal reaction conditions of recombinant escherichia coli expressing penicillium chrysogenum 8,11-lds for the production of 8,11-dihydroxy-9,12(z,z)-octadecadienoic acid (8,11-dihode), 8,11-dihydrox ... | 2016 | 27997074 |
| characterization and expression of the antifungal protein from monascus pilosus and its distribution among various monascus species. | monascus species are traditionally used for food preservation. this study used the disc diffusion method to verify the antifungal activity of protein extracted from monascus pilosus bcrc38072 against 15 fungal pathogens. an antifungal protein, designated as mafp1, was successfully purified and confirmed through n-terminal sequencing. to further explore the antifungal gene, a mafp1 gene that is similar to that of pgafp from penicillium chrysogenum was cloned from m. pilosus bcrc38072. according t ... | 2016 | 26803705 |
| vacuolar serine protease is a major allergen of fusarium proliferatum and an ige-cross reactive pan-fungal allergen. | fusarium species are among prevalent airborne fungi and causative agents of human respiratory atopic disorders. we previously identified a 36.5-kda f. proliferatum component recognized by ige antibodies in 9 (53%) of the 17 f. proliferatum-sensitized atopic serum samples. the purpose of this study is to characterize the 36.5-kda allergen of f. proliferatum. | 2016 | 27334782 |
| new promoters for strain engineering of penicillium chrysogenum. | filamentous fungi such as aspergillus and penicillium are widely used as hosts for the industrial products such as proteins and secondary metabolites. although filamentous fungi are versatile in recognizing transcriptional and translational elements present in genes from other filamentous fungal species, only few promoters have been applied and compared in performance so far in penicillium chrysogenum. therefore, a set of homologous and heterologous promoters were tested in a reporter system to ... | 2016 | 26701309 |
| silver nanoparticles: a mechanism of action on moulds. | silver nanoparticles (agnps) are widely used in all branches of industry. however, their mechanisms of action towards moulds have not been studied yet. thus we conducted this study in which we have used laser desorption/ionization time-of-flight mass spectrometry (ldi-tof-ms) analysis to determine metabolomic changes, and microscopic analysis (transmission electron microscopy, fluorescent microscopy) to observe changes in mould cells. the agnp treatment caused the downregulation of 162 (15 ppm) ... | 2016 | 27896349 |
| antifungal, anticancer and aminopeptidase inhibitory potential of a phenazine compound produced by lactococcus bsn307. | a bioactive compound was purified from the culture medium of a new strain of lactococcus bsn307 by solvent extraction followed by chromatographic techniques. this bioactive compound was identified to belong to phenazine class of compounds by ms, nmr and ftir. the phenazine compound showed antifungal activity against aspergillus niger, penicillium chrysogenum as well as fusarium oxysporum by disc diffusion assay in addition to antioxidant potential as demonstrated by dpph scavenging assay. the co ... | 2016 | 27784936 |
| winery biomass waste degradation by sequential sonication and mixed fungal enzyme treatments. | to increase the efficiency of winery-derived biomass biodegradation, grape pomace was ultrasonicated for 20min in the presence of 0.25m, 0.5mand1.0mkoh and 1.0mnaoh. this was followed by treatment with a 1:1 (v/v) mix of crude enzyme preparation derived from phanerochaete chrysosporium and trametes versicolor for 18h and a further 18h treatment with a 60:14:4:2 percent ratio combination of enzymes derived from aspergillus niger: penicillium chrysogenum: trichoderma harzianum: p. citrinum, repsec ... | 2016 | 27599392 |
| shedding light on aspergillus niger volatile exometabolome. | an in-depth exploration of the headspace content of aspergillus niger cultures was performed upon different growth conditions, using a methodology based on advanced multidimensional gas chromatography. this volatile fraction comprises 428 putatively identified compounds distributed over several chemical families, being the major ones hydrocarbons, alcohols, esters, ketones and aldehydes. these metabolites may be related with different metabolic pathways, such as amino acid metabolism, biosynthes ... | 2016 | 27264696 |
| transcriptomic responses of mixed cultures of ascomycete fungi to lignocellulose using dual rna-seq reveal inter-species antagonism and limited beneficial effects on cazyme expression. | gaining new knowledge through fungal monoculture responses to lignocellulose is a widely used approach that can lead to better cocktails for lignocellulose saccharification (the enzymatic release of sugars which are subsequently used to make biofuels). however, responses in lignocellulose mixed cultures are rarely studied in the same detail even though in nature fungi often degrade lignocellulose as mixed communities. using a dual rna-seq approach, we describe the first study of the transcriptio ... | 2016 | 27150814 |
| antimicrobial potentials of different solvent extracted samples from physalis ixocarpa. | the present study investigates the antimicrobial activities of different solvent extracted samples isolated from different parts of physalis ixocarpa through disc diffusion assay using three different concentrations. statistical analysis of the data revealed that different parts of the plant showed varying degree of inhibition against different bacteria at different concentrations. different solvent extracted samples from the calyx showed inhibitory activity against most of the bacteria under st ... | 2016 | 27087074 |
| computing the various pathways of penicillin synthesis and their molar yields. | more than 80 years after its discovery, penicillin is still a widely used and commercially highly important antibiotic. here, we analyse the metabolic network of penicillin synthesis in penicillium chrysogenum based on the concept of elementary flux modes. in particular, we consider the synthesis of the invariant molecular core of the various subtypes of penicillin and the two major ways of incorporating sulfur: transsulfuration and direct sulfhydrylation. 66 elementary modes producing this inva ... | 2016 | 26134880 |
| increased chitin biosynthesis contributes to the resistance of penicillium polonicum against the antifungal protein pgafp. | antifungal proteins from molds have been proposed as a valuable tool against unwanted molds, but the resistance of some fungi limits their use. resistance to antimicrobial peptides has been suggested to be due to lack of interaction with the mold or to a successful response. the antifungal protein pgafp produced by penicillium chrysogenum inhibits the growth of various ascomycetes, but not penicillium polonicum. to study the basis for resistance to this antifungal protein, localization of pgafp ... | 2016 | 26446386 |
| observability analysis of biochemical process models as a valuable tool for the development of mechanistic soft sensors. | by enabling the estimation of difficult-to-measure target variables using available indirect measurements, mechanistic soft sensors have become important tools for various bioprocess monitoring and control scenarios. despite promising higher process efficiencies and increased process understanding, widespread application of soft sensors has been stalled by uncertainty about the feasibility and reliability of their estimations given present process analytical constraints. observability analysis c ... | 2016 | 26404038 |
| molecular characterization of patulin producing and non-producing penicillium species in apples from morocco. | the isolation of patulin-producing penicillia in apples collected in different markets in four localities in morocco is reported. fungi were identified by β-tubulin sequencing and further characterized using a specific pcr-based method targeting the isoepoxydon dehydrogenase (idh) gene to discriminate between patulin-producing and non-producing strains. production of patulin was also evaluated using standard cultural and biochemical methods. results showed that 79.5% of contaminant fungi belonge ... | 2016 | 26513254 |
| high throughput de novo rna sequencing elucidates novel responses in penicillium chrysogenum under microgravity. | in this study, the transcriptional alterations in penicillium chrysogenum under simulated microgravity conditions were analyzed for the first time using an rna-seq method. the increasing plethora of eukaryotic microbial flora inside the spaceship demands the basic understanding of fungal biology in the absence of gravity vector. penicillium species are second most dominant fungal contaminant in international space station. penicillium chrysogenum an industrially important organism also has the p ... | 2016 | 26603994 |
| effects of penicillium chrysogenum var. halophenolicum on kraft lignin: color stabilization and cytotoxicity evaluation. | wood industries and agricultural crops generate an inexhaustible supply of by-products like lignin, which constitutes an environmental problem. increasing efforts have been done to find new applications for lignin. one of them is as a food additive, but its chemical nature makes it sensitive to browning which constitutes a major drawback for this type of lignin application. in the present study we are documenting how color stabilization of a commercial kraft lignin was achieved after the treatme ... | 2016 | 28330172 |
| the indole alkaloid meleagrin, from the olive tree endophytic fungus penicillium chrysogenum, as a novel lead for the control of c-met-dependent breast cancer proliferation, migration and invasion. | fungi of the genus penicillium produce unique and chemically diverse biologically active secondary metabolites, including indole alkaloids. the role of dysregulated hepatocyte growth factor (hgf) and its receptor, c-met, in the development and progression of breast carcinoma is documented. the goal of this work is to explore the chemistry and bioactivity of the secondary metabolites of the endophytic penicillium chrysogenum cultured from the leaf of the olive tree olea europea, collected in its ... | 2016 | 26692349 |
| characterization of a novel 8r,11s-linoleate diol synthase from penicillium chrysogenum by identification of its enzymatic products. | to identify novel fatty acid diol synthases, putative candidate sequences from penicillium species were analyzed, and hydroxy fatty acid production by crude penicillium enzyme extracts was assessed. penicillium chrysogenum was found to produce an unknown dihydroxy fatty acid, a candidate gene implicated in this production was cloned and expressed, and the expressed enzyme was purified. the product obtained by the reaction of the purified enzyme with linoleic acid was identified as 8r,11s-dihydro ... | 2016 | 26681780 |
| evolutionary formation of gene clusters by reorganization: the meleagrin/roquefortine paradigm in different fungi. | the biosynthesis of secondary metabolites in fungi is catalyzed by enzymes encoded by genes linked in clusters that are frequently co-regulated at the transcriptional level. formation of gene clusters may take place by de novo assembly of genes recruited from other cellular functions, but also novel gene clusters are formed by reorganization of progenitor clusters and are distributed by horizontal gene transfer. this article reviews (i) the published information on the roquefortine/meleagrin/neo ... | 2016 | 26668029 |
| crispr/cas9 based genome editing of penicillium chrysogenum. | crispr/cas9 based systems have emerged as versatile platforms for precision genome editing in a wide range of organisms. here we have developed powerful crispr/cas9 tools for marker-based and marker-free genome modifications in penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. the developed crispr/cas9 toolbox is highly flexible and allows editing of new targets with minimal cloning efforts. the cas9 protein and the sgrna can be either delivered during t ... | 2016 | 27072635 |
| penimethavone a, a flavone from a gorgonian-derived fungus penicillium chrysogenum. | a novel flavone, penimethavone a (1), possessing a rare unique methyl group at ring-b, was isolated from the fungus penicillium chrysogenum cultured from a gorgonian carijoa sp. collected from the south china sea. the structure was elucidated by extensive spectroscopic analysis and by comparison with related known compound. compound 1 showed selective and moderate cytotoxicity against cervical cancer (hela) and rhabdomyosarcoma cell lines with ic50 values of 8.41 and 8.18 μm, respectively. | 2016 | 26998621 |
| comprehensive profiling of proteome changes provide insights of industrial penicillium chrysogenum during pilot and industrial penicillin g fermentation. | the intracellular proteomes of the penicillium chrysogenum throughout pilot and industrial processes were investigated by using 2-de combined with maldi-tof-tof ms, respectively. we detected a total of 223 spots corresponding to 154 proteins and 231 spots corresponding to 157 proteins throughout pilot and industrial processes, respectively. the levels of glyceraldehyde-3-phosphate dehydrogenase increased (5.1- and 2.5-fold) under the pilot process, while its levels were no significant changes un ... | 2016 | 26961188 |
| at-line determination of spore inoculum quality in penicillium chrysogenum bioprocesses. | spore inoculum quality in filamentous bioprocesses is a critical parameter influencing pellet morphology and, consequently, process performance. it is essential to determine the concentration of viable spores before inoculation, to implement quality control and decrease batch-to-batch variability. the ability to assess the spore physiologic status with close-to-real time resolution would offer interesting perspectives enhanced process analytical technology (pat) and quality by design (qbd) strat ... | 2016 | 26820651 |