Publications

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aspergillus oryzae lectin induces anaphylactoid edema and mast cell activation through its interaction with fucose of mast cell-bound non-specific ige.we investigated whether aspergillus oryzae lectin (aol), a fucose-specific lectin, induces anaphylactoid reactions and mast cell activation. the injection of aol into footpads of mice produced a dose-related acute paw edema. the aol-induced edema was attenuated by predose of histamine h1 receptor blocker or pretreatment of the lectin with fucose before injection, and was not observed in scid and mast cell-deficient wbb6f1-w/wv mice. these results suggested that the aol-induced anaphylactoid reac ...201121790704
a novel biocatalytic approach to acetylation of 1-ß-d: -arabinofuranosylcytosine by aspergillus oryzae whole cell in organic solvents.biocatalytic acylation of 1-ß-d: -arabinofuranosylcytosine (ara-c) was developed using whole cell of aspergillus oryzae as a novel catalyst. (13)c nuclear magnetic resonance (nmr) analysis indicated that the whole-cell biocatalyst had more specific activity toward the 3'-hydroxyl group than 5'-hydroxyl group among the available hydroxyl groups in sugar moiety of ara-c. except for glucose and maltose, 11 carbon sources supplemented to basal media, including spans, tweens, olive oil and oleic acid ...201121755282
cellulolytic enzymes production via solid-state fermentation: effect of pretreatment methods on physicochemical characteristics of substrate.we investigated the effect of pretreatment on the physicochemical characteristics-crystallinity, bed porosity, and volumetric specific surface of soybean hulls and production of cellulolytic enzymes in solid-state fermentation of trichoderma reesei and aspergillus oryzae cultures. mild acid and alkali and steam pretreatments significantly increased crystallinity and bed porosity without significant change inholocellulosic composition of substrate. crystalline and porous steam-pretreated soybean ...201121755043
proteomics of eukaryotic microorganisms: the medically and biotechnologically important fungal genus aspergillus.fungal species of the genus aspergillus play significant roles as model organisms in basic research, as "cell factories" for the production of organic acids, pharmaceuticals or industrially important enzymes and as pathogens causing superficial and invasive infections in animals and humans. the release of the genome sequences of several aspergillus sp. has paved the way for global analyses of protein expression in aspergilli including the characterisation of proteins, which have not designated a ...201121726053
peroxisomes are involved in biotin biosynthesis in aspergillus and arabidopsis.among the eukaryotes only plants and a number of fungi are able to synthesize biotin. although initial events leading to the biosynthesis of biotin remain largely unknown, the final steps are known to occur in the mitochondria. here we deleted the aopex5 and aopex7 genes encoding the receptors for peroxisomal targeting signals pts1 and pts2, respectively, in the filamentous fungus aspergillus oryzae. in addition to exhibiting defects in the peroxisomal targeting of either pts1 or pts2 proteins, ...201121730067
direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing saccharomyces cerevisiae cells.the cost of the lignocellulose-hydrolyzing enzymes used in the saccharification process of ethanol production from biomass accounts for a relatively high proportion of total processing costs. cell surface engineering technology has facilitated a reduction in these costs by integrating saccharification and fermentation processes into a recombinant microbe strain expressing heterologous enzymes on the cell surface. we constructed a recombinant saccharomyces cerevisiae that not only hydrolyzed hemi ...201121741417
[promotive excretion of causative agents of yusho by fermented brown rice with aspergillus oryze in yusho patients].forty two years have passed since the outbreak of kanemi rice oil poisoning, namely, yusho in the western japan. however, even now the yusho patients have been still suffering from several objective and subjective symptoms. in order to improve or, if possible, to cure such symptoms, the most important therapeutic treatment is considered to actively excrete the causative agents, that is, polychlorinated dibenzofurans (pcdfs) from the bodies of the patients and to reduce their body burdens. in rat ...201121706891
a carrier fusion significantly induces unfolded protein response in heterologous protein production by aspergillus oryzae.in heterologous protein production by filamentous fungi, target proteins are expressed as fusions with homologous secretory proteins, called carriers, for higher production yields. although carrier fusion is thought to overcome the bottleneck in transcriptional and (post)translational processes during heterologous protein production, there is limited knowledge of its physiological effects on the host strain. in this study, we performed dna microarray analysis by comparing gene expression pattern ...201121822643
isolation of a novel promoter for efficient protein expression by aspergillus oryzae in solid-state culture.a novel promoter from a hemolysin-like protein encoding the gene, hlya, was characterized for protein overexpression in aspergillus oryzae grown in solid-state culture. using endo-1,4-+¦-glucanase from a. oryzae (cela) as the reporter, promoter activity was found to be higher than that of the +¦-amylase (amya) and manganese superoxide dismutase (sodm) genes not only in wheat bran solid-state culture but also in liquid culture. expression of the a. oryzae endoglucanase celb and two heterologous e ...201121732241
identification of potential cell wall component that allows taka-amylase a adsorption in submerged cultures of aspergillus oryzae.we observed that +¦-amylase (taka-amylase a; taa) activity in the culture broth disappeared in the later stage of submerged cultivation of aspergillus oryzae. this disappearance was caused by adsorption of taa onto the cell wall of a. oryzae and not due to protein degradation by extracellular proteolytic enzymes. to determine the cell wall component(s) that allows taa adsorption efficiently, the cell wall was fractionated by stepwise alkali treatment and enzymatic digestion. consequently, alkali ...201121687962
cloning and expression of a tyrosinase from aspergillus oryzae in yarrowia lipolytica: application in l: -dopa biotransformation.amplification of the tyrosinase gene (melo) from the genomic dna of aspergillus oryzae ncim 1212 yielded a 1.6-kb product. this gene was cloned into pylex1, and the resulting ptyro-ylex1 vector was transformed in yarrowia lipolytica strain po1g. a clone displaying the highest specific activity for tyrosinase (10.94-áu/mg) was used for obtaining the complementary dna (cdna) and for protein expression studies. cdna sequence analysis indicated the splicing of an intron present in the melo gene by p ...201121687965
identification and comparison of cutinases for synthetic polyester degradation.cutinases have been exploited for a broad range of reactions, from hydrolysis of soluble and insoluble esters to polymer synthesis. to further expand the biotechnological applications of cutinases for synthetic polyester degradation, we perform a comparative activity and stability analysis of five cutinases from alternaria brassicicola (abc), aspergillus fumigatus (afc), aspergillus oryzae (aoc), humicola insolens (hic), and the well-characterized fusarium solani (fsc). of the cutinases, hic dem ...201121713515
influence of dietary ingredients on in vitro inflammatory response of intestinal porcine epithelial cells challenged by an enterotoxigenic escherichia coli (k88).enterotoxigenic escherichia coli (etec) k88 is the main bacterial cause of diarrhea in piglets around weaning and the adhesion of etec to the intestinal mucosa is a prerequisite step for its colonization. in this study, the adhesion of a fimbriated etec and a non-fimbriated e. coli (nfec) to the intestinal cells and the activation of the innate immune system were evaluated using a porcine intestinal epithelial cell line (ipec-j2). the impact of several feedstuffs (wheat bran (wb); casein glycoma ...201121944732
Development of fluorescent probes for the detection of fucosylated N-glycans using an Aspergillus oryzae lectin.The a(1,6)-fucose attached to the core N-glycan (core fucose) of glycoproteins has been known to play essential roles in various pathophysiological events, including oncogenesis and metastasis. Aspergillus oryzae lectin (AOL) encoded by the fleA gene has been reported to bind to N-glycans containing core fucose. The fleA gene encoding AOL was cloned into an Escherichia coli expression vector and then fused with genes of fluorescent proteins for production of fusion proteins. The resulting FleA-f ...201121892597
Effects of fermented brown rice on the intestinal environments in healthy adult.The aim of this study is to investigate the prebiotic effects of brown rice fermented by Aspergillus oryzae (FBRA) on the intestinal environment in vitro and in healthy adults.201121921425
lack of endoplasmic reticulum 1,2-α-mannosidase activity that trims n-glycan man(9)glcnac(2) to man(8)glcnac(2) isomer b in a mane gene disruptant of aspergillus oryzae.the gene mane, encoding a probable class i endoplasmic reticulum 1,2-α-mannosidases (er-man), was identified from the filamentous fungus aspergillus oryzae due to similarity to orthologs. it removes a single mannose residue from man(9)glcnac(2), generating man(8)glcnac(2) isomer b. disruption of mane caused drastic decreases in er-man activity in a. oryzae microsomes.201122169093
comparative study the effects of solid-state fermentation with three filamentous fungi on the total phenolics content (tpc), flavonoids and antioxidant activities of subfractions from oats (avena sativa l.).the aim of present work was to investigate the effect of solid-state fermentation with filamentous fungi (aspergillus oryzae var. effuses, aspergillus oryzae, and aspergillus niger) on total phenolics content (tpc), flavonoids and antioxidant activities of four subfractions of oat, namely n-hexane, ethyl acetate (ea), n-butanol and water subfraction, and compare them with their corresponding subfractions of unfermented oat. the tpc and total flavonoids increased dramatically, especially in ea su ...201122136169
volatile components of the enzyme-ripened sufu, a chinese traditional fermented product of soy bean.in the present study, sufu, a soft cheese-like oriental fermented food, was prepared by ripening the salted-tofu cubes in aspergillus oryzae-fermented soybean-rice koji at 37°c for 16days (16-day sufu). sufu was further held at room temperature for another 30days (46-day sufu). the volatile components of the non-fermented tofu cubes and the sufu products were identified and quantified by gc and gc-ms. a total of 70 volatile compounds including 20 aldehydes, 18 alcohols, 16 esters, 5 ketones, 5 a ...201122051578
heterologous expression system in aspergillus oryzae for fungal biosynthetic gene clusters of secondary metabolites.fungal secondary metabolites have been considered promising resources in the search for novel bioactive compounds. given the high potential of fungi as genetic resources, it is essential to find an efficient way to link biosynthetic genes to the product in a heterologous system, because many genes for the secondary metabolite in the original strain are silent under standard laboratory conditions. in a previous study, we constructed a heterologous expression system for a biosynthetic gene cluster ...201122083274
enzymatic activity and flavor compound production in fermented silver carp fish paste inoculated with douchi starter culture.silver carp fish pastes inoculated with or without a douchi starter culture containing live aspergillus oryzae were fermented for 30 days to produce two different fermented products, designated as culf and conf, respectively. protein degradation and flavor compound production during the course of fermentation were monitored. proteolytic activity, generally higher in culf than in conf (p < 0.05) and dominated by acidic and serine proteases, declined to an overall minimum after 30 days. myosin in ...201122148721
molecular and morphological identification of fungal species isolated from bealmijang meju.bealmijang is a short-term aged paste made from meju, which is a brick of fermented soybeans and other ingredients. different types of bealmijang are available depending on the geographic region or ingredients used. however, no study has clarified the microbial diversity of these types. we identified 17 and 14 fungal species from black soybean meju (bsm) and buckwheat meju (bwm), respectively, on the basis of morphology, culture characteristics, and internal transcribed spacer and beta-tubulin g ...201122210613
identification, cloning, and characterization of β-glucosidase from ustilago esculenta.hydrolytic enzymes responsible for laminarin degradation were found to be secreted during growth of ustilago esculenta on laminarin. an enzyme involved in laminarin degradation was purified by assaying release of glucose from laminaribiose. ion-exchange chromatography of the culture filtrate followed by size-exclusion chromatography yielded a 110-kda protein associated with laminaribiose hydrolysis. lc/ms/ms analysis of the 110-kda protein identified three peptide sequences that shared significa ...201121850431
conserved and specific responses to hypoxia in aspergillus oryzae and aspergillus nidulans determined by comparative transcriptomics.hypoxia imposes stress on filamentous fungi that require oxygen to proliferate. global transcription analysis of aspergillus oryzae grown under hypoxic conditions found that the expression of about 50% of 4,244 affected genes was either induced or repressed more than 2-fold. a comparison of these genes with the hypoxically regulated genes of aspergillus nidulans based on their predicted amino acid sequences classified them as bi-directional best hit (bbh), one-way best hit (extra homolog, eh), a ...201122170104
total biosynthesis of diterpene aphidicolin, a specific inhibitor of dna polymerase α: heterologous expression of four biosynthetic genes in aspergillus oryzae.clustering of biosynthetic genes for producing fungal secondary metabolites, which frequently consist of less than ten genes, has been recognized with numerous genomes. the heterologous expression of whole genes in the clusters will therefore produce various types of natural products when using a suitable fungal host. we introduced the whole gene cluster for the biosynthesis of diterpene aphidicolin into the fungal quadruple auxotrophic host, aspergillus oryzae, by using four different vectors ( ...201121897020
formate oxidase, an enzyme of the glucose-methanol-choline oxidoreductase family, has a his-arg pair and 8-formyl-fad at the catalytic site.formate oxidase of aspergillus oryzae rib40 contains an 8-replaced fad with molecular mass of 799 as cofactor. the ¹h-nmr spectrum of the cofactor fraction obtained from the enzyme indicated that the 8-replaced fad in the fraction was 8-formyl-fad, present in open form and hemiacetal form. the oxidation-reduction potentials of the open and hemiacetal forms were estimated by cyclic voltammetry to be -47 and -177 mv vs. normal hydrogen electrode respectively. the structure of the enzyme was constr ...201121897046
Identification and characterization of a putative basic helix-loop-helix transcription factor involved in the early stage of conidiophore development in Aspergillus oryzae.The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes. HLH proteins form homo- and/or heterodimers with other HLH proteins and bind to E-box motifs. The regulation and functions of these proteins can be complex due to their bifunctional roles as activators and repressors of gene transcription. In this study, we isolated and characterized a novel predicted bHLH protein-encoding gene, AO090023000902, designated ecdR (early conidioph ...201122008745
indigestible dextrin is an excellent inducer for α-amylase, α-glucosidase and glucoamylase production in a submerged culture of aspergillus oryzae.α-amylase activities of aspergillus oryzae grown on dextrin or indigestible dextrin were 7·8 and 27·7 u ml(-1), respectively. glucoamylase activities of the cultures grown on dextrin or indigestible dextrin were 5·4 and 301 mu ml(-1), respectively. the specific glucoamylase production rate in indigestible dextrin batch culture reached 1·35 u g dw(-1) h(-1). in contrast, biomass concentration of a. oryzae in indigestible dextrin culture was 35% of that in dextrin culture. thus, the culture method ...201122009575
Multifunctional nanoadditives for the thermodynamic and kinetic stabilization of enzymes.Stabilization of enzymes has become a major focus in the quest to improve the activity, sustainability and recyclability of enzymes for their successful integration into both industry and medicine. Here, we describe the kinetic and thermodynamic stabilization of a variety of enzymes in the presence of cationic multifunctional polymeric nanoparticles.201121897968
study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from aspergillus oryzae.the study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. the synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (hplc). according to the reaction of s-6-a catalyzed by fructosyltransferase from aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, ph, substrate and enzyme concentration in the rea ...201120643232
gene cloning and enzyme structure modeling of the aspergillus oryzae n74 fructosyltransferase.the fructooligosaccharides (fos) represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. recently, the strain aspergillus oryzae n74 was reported as a potential microorganism for the industrial production of fos, due to its high yields of fos production. in this work, we used a pcr-cloning strategy to clone the a. oryzae n74 ftase gene as a previous step for recombinant enzyme production. ftase showed a 1630 bp size with a 99% similarity wi ...201120563857
protease inhibitor from moringa oleifera with potential for use as therapeutic drug and as seafood preservative.protease inhibitors are well known to have several applications in medicine and biotechnology. several plant sources are known to return potential protease inhibitors. in this study plants belonging to different families of leguminosae, malvaceae, rutaceae, graminae and moringaceae were screened for the protease inhibitor. among them moringa oleifera, belonging to the family moringaceae, recorded high level of protease inhibitor activity after ammonium sulfate fractionation. m. oleifera, which g ...201123961135
kinetic analysis of batch ethanol acetylation in isothermal non-stationary multiphase systems by lyophilized mycelium of aspergillus oryzae.a relatively complex network of reactions has been investigated, using as a network model the isothermal batch esterification of acetic acid with ethanol in n-heptane catalyzed by lyophilized mycelium of aspergillus oryzae. the kinetic analysis was firstly carried out on the whole system, without any simplification, by means of the well-known integral method. owing to the poor results obtained by this way, we developed an alternative approach, combining initial rates and integral analysis and re ...201124031645
improved heterologous protein production by a tripeptidyl peptidase gene (aosedd) disruptant of the filamentous fungus aspergillus oryzae.proteolytic degradation is one of the serious bottlenecks limiting the yields of heterologous protein production by aspergillus oryzae. in this study, we selected a tripeptidyl peptidase gene aosedd (ao090166000084) as a candidate potentially degrading the heterologous protein, and performed localization analysis of the fusion protein aosedd-egfp in a. oryzae. as a result, the aosedd-egfp was observed in the septa and cell walls as well as in the culture medium, suggesting that aosedd is a secre ...201222878738
contribution ratios of amya, amyb, amyc genes to high-level α-amylase expression in aspergillus oryzae.aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain rib40 has three α-amylase genes (amya, amyb, and amyc). however, there is no information on the contribution ratios of individual α-amylase genes to total expression. in this study, we generated single, double, and triple disruptants of α-amylase genes by employing a strain (δligd) with high gene-targeting efficiency and pyrg marker recycling in a. oryzae. all the disruptants showed reduced activities of α- ...201222878189
degradation of terbuthylazine, difenoconazole and pendimethalin pesticides by selected fungi cultures.contamination of waters by xenobiotic compounds such as pesticides presents a serious environmental problem with substantial levels of pesticides now contaminating european water resources. the aim of this work was to evaluate the ability of the fungi fusarium oxysporum, aspergillus oryzae, lentinula edodes, penicillium brevicompactum and lecanicillium saksenae, for the biodegradation of the pesticides terbuthylazine, difenoconazole and pendimethalin in batch liquid cultures. these pesticides ar ...201222878100
isolation and identification of two novel umami and umami-enhancing peptides from peanut hydrolysate by consecutive chromatography and maldi-tof/tof ms.peanut hydrolysate produced by crude protease extract from aspergillus oryzae hn 3.042 was found to elicit intense umami and umami-enhancing effect. taste profiles, amino acid and organic acid composition of peanut hydrolysate and its separation fractions by ultrafiltration were evaluated. the results revealed that peanut hydrolysate was mainly low molecular weight compounds. fractions of 1-3 kda and below 1 kda prominently contributed to the umami taste and umami-enhancing effect of the peanut ...201222868117
common and distant structural characteristics of feruloyl esterase families from aspergillus oryzae.feruloyl esterases (faes) are important biomass degrading accessory enzymes due to their capability of cleaving the ester links between hemicellulose and pectin to aromatic compounds of lignin, thus enhancing the accessibility of plant tissues to cellulolytic and hemicellulolytic enzymes. faes have gained increased attention in the area of biocatalytic transformations for the synthesis of value added compounds with medicinal and nutritional applications. following the increasing attention on the ...201222745763
metabolomics-based optimal koji fermentation for tyrosinase inhibition supplemented with astragalus radix.the present study was focused on improving the quality of rice koji by fermentation with a selected aspergillus oryzae strain and a plant astragalus radix. a. oryzae kccm 60345 was used as main inoculant and the astragalus radix was added as supplement in rice koji preparation. lc-ms based metabolite analysis and tyrosinase inhibitory activities were studied for different time periods. a. oryzae kccm 60345 fermented rice koji supplemented with astragalus showed higher tyrosinase inhibition activ ...201222738950
enzymatic characteristics of a recombinant neutral protease i (rnpi) from aspergillus oryzae expressed in pichia pastoris.a truncated neutral protease i (npi) from aspergillus oryzae 3.042 was expressed in pichia pastoris with a high enzyme yield of 43101 u/ml. its optimum ph was about 8.0, and it was stable in the ph range of 5.0-9.0. its optimum temperature was about 55 °c and retained >90% activity at 50 °c for 120 min. recombinant npi (rnpi) was inhibited by cu(2+) and edta. eight cleavage sites of rnpi in oxidized insulin b-chain were determined by mass spectrometry, and five of them had high hydrophobic amino ...201223136814
isolation and characterization of the mhea (most highly expressed) gene of aspergillus oryzae.the amino acid sequence of the mhea gene of aspergillus oryzae encodes a putative metallothionein-like protein 1. the size of the mhea transcript was 497 nt and the mhea promoter was induced by glucose, consistent with results of analysis by northern hybridization and with the pdca promoter, respectively.201223115516
hyphal heterogeneity in aspergillus oryzae is the result of dynamic closure of septa by woronin bodies.hyphae of higher fungi are compartmentalized by septa. these septa contain a central pore that allows for inter-compartmental and inter-hyphal cytoplasmic streaming. the cytoplasm within the mycelium is therefore considered to be a continuous system. in this study, however, we demonstrate by laser dissection that 40% of the apical septa of exploring hyphae of aspergillus oryzae are closed. closure of septa correlated with the presence of a peroxisome-derived organelle, known as woronin body, nea ...201223106143
a toolkit for heterologous expression of metabolic pathways in aspergillus oryzae.much has been learned about the activities of the key enzymes involved in eukaryotic natural product synthesis by isolating the relevant genes and expressing them in a suitable foreign host. aspergillus oryzae has proved to be an amenable host for the functional analysis of megasynthases from other fungi, but secondary metabolites are often the products of suites of enzymes, and understanding their biosynthesis requires simultaneous expression of several genes. this chapter describes the develop ...201223084942
reconstitution of a secondary metabolite biosynthetic pathway in a heterologous fungal host.expression of multiple genes involved in a particular metabolic pathway in a heterologous host facilitates the study of fungal secondary metabolite biosynthesis and production of useful compounds. two plasmids with different selection markers, argb and the pyrithiamine resistance marker, are used to transform aspergillus oryzae allowing for expression of up to three genes simultaneously.201223065616
manr, a novel zn(ii)2cys6 transcriptional activator, controls the β-mannan utilization system in aspergillus oryzae.fungal endo-β-mannanases (β-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of β-mannanases has been identified in fungi or other eukaryotic cells to date. to identify a transcriptional regulator of β-mannanases in aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced β-mannanase activity by using konjac glucomannan as the substrate, and manr, a zn(ii)(2)cys(6) type dna binding protein was ...201223063954
serine-type carboxypeptidase kexa of aspergillus oryzae has broader substrate specificity than saccharomyces cerevisiae kex1 and is required for normal hyphal growth and conidiation.aspergillus oryzae has an ortholog of saccharomyces cerevisiae kex1, termed kexa. a truncated form of kexa protein showed serine-type carboxypeptidase activity and somewhat broader substrate specificity than kex1 protease. furthermore, our results indicated that kexa is required for normal growth of a. oryzae and that it might be involved in hyphal branching.201222961905
production of polygalacturonases by aspergillus oryzae in stirred tank and internal- and external-loop airlift reactors.the production of endo- and exo-polygalacturonase (pg) by aspergillus oryzae was assessed in stirred tank reactors (strs), internal-loop airlift reactors (ilars) and external-loop airlift reactors (elars). for str production, we compared culture media formulated with either pectin (wbe) or partially hydrolyzed pectin. the highest enzyme activities were obtained in medium that contained 50% pectin in hydrolyzed form (wbe5). pg production in the three reactor types was compared for wbe5 and low sa ...201222940313
kinetic analysis, expression pattern, and production of a recombinant fungal protease inhibitor of tasar silkworm antheraea mylitta.antheraea mylitta, a tasar silk-producing insect of saturniidae family, expresses a fungal protease inhibitor named as a. mylitta fungal protease inhibitor-1 (amfpi-1). amfpi-1 inhibits alkaline protease of aspergillus oryzae but its mechanism of action is not known. to understand the mode of inhibition of amfpi-1 against the fungal protease, it was purified from the hemolymph of a. mylitta larvae and inhibitory activity against a. oryzae protease was studied. kinetic analysis of purified amfpi- ...201222935928
draft genome sequence of aspergillus oryzae strain 3.042.aspergillus oryzae is the most important fungus for the traditional fermentation in china and is particularly important in soy sauce fermentation. we report the 36,547,279-bp draft genome sequence of a. oryzae 3.042 and compared it to the published genome sequence of a. oryzae rib40.201222933657
identification of a gene involved in the synthesis of a dipeptidyl peptidase iv inhibitor in aspergillus oryzae.wyk-1 is a dipeptidyl peptidase iv inhibitor produced by aspergillus oryzae strain ao-1. because wyk-1 is an isoquinoline derivative consisting of three l-amino acids, we hypothesized that a nonribosomal peptide synthetase was involved in its biosynthesis. we identified 28 nonribosomal peptide synthetase genes in the sequenced genome of a. oryzae rib40. these genes were also identified in ao-1. among them, ao090001000009 (wykn) was specifically expressed under wyk-1-producing conditions in ao-1. ...201222843525
removal of off-flavors from sea tangle (laminaria japonica) extract by fermentation with aspergillus oryzae.this study was conducted to reduce off-flavors from sea tangle (laminaria japonica) extract since the typical odor of seaweed is an impediment to consumption of seaweed products. fifty-six odor compounds emitted from the extract were identified using a thermal desorbor and gas chromatography-mass spectrometry. on the basis of expected odor intensity (eoi), isovaleric acid (41.2%), allyl isothiocyanate (22.6%), octanal (11.1%) and acetaldehyde (10.6%) were responsible for about 85.5% of total odo ...201222840201
comparison of expression and enzymatic properties of aspergillus oryzae lysine aminopeptidases apsa and apsb.the apsa and apsb genes encoding family m1 aminopeptidases were identified in the industrial fungus aspergillus oryzae. the apsb was transcriptionally up-regulated up to 2.5-fold in response to the deprivation of nitrogen or carbon sources in growth media, while up-regulation of apsa was less significant. the encoded proteins were bacterially expressed and purified to characterize their enzymatic properties. apsa and apsb were optimally active at ph 7.0 and 35 °c and stable at ph ranges of 6-10 ...201222806190
solid-state fermentation of soybean and corn processing coproducts for potential feed improvement.two agro-industrial coproducts, soybean cotyledon fiber and distiller's dried grains with solubles (ddgs), were used as substrates to evaluate the effect of coculturing three different fungi, aspergillus oryzae, trichoderma reesei, and phanerochaete chrysosporium, on enzyme production by solid-state fermentation (ssf). when soybean fiber was used as the substrate, a maximum xylanase activity of 757.4 iu/g and a cellulase activity of 3.2 iu/g were achieved with the inoculation and incubation of t ...201222799754
the evolutionary imprint of domestication on genome variation and function of the filamentous fungus aspergillus oryzae.the domestication of animals, plants, and microbes fundamentally transformed the lifestyle and demography of the human species [1]. although the genetic and functional underpinnings of animal and plant domestication are well understood, little is known about microbe domestication [2-6]. here, we systematically examined genome-wide sequence and functional variation between the domesticated fungus aspergillus oryzae, whose saccharification abilities humans have harnessed for thousands of years to ...201222795693
cloning and expression of the aspergillus oryzae glucan 1,3-beta-glucosidase a (exga) in pichia pastoris.the glucan 1,3-beta-glucosidase a gene (exga) from aspergillus oryzae and fused to the saccharomyces cerevisiae signal peptide (α-factor) was expressed under the control of either a constitutive (gap) or an inducible (aox1) promoter in pichia pastoris. a 1.4-fold higher extracellular enzyme activity (2 u/ml) was obtained using the aox1 inducible expression system than with the gap constitutive promoter (1.4 u/ml). the purified recombinant exga enzyme, with a yield of 10 mg protein/l culture supe ...201222782265
population-based study reveals new risk-stratification biomarker panel for barrett's esophagus.the risk of progression of barrett's esophagus (be) to esophageal adenocarcinoma (eac) is low and difficult to calculate. accurate tools to determine risk are needed to optimize surveillance and intervention. we assessed the ability of candidate biomarkers to predict which cases of be will progress to eac or high-grade dysplasia and identified those that can be measured in formalin-fixed tissues.201222771507
jewelry boxes contaminated by aspergillus oryzae: an occupational health risk?in 2009, 100,000 jewelry boxes, manufactured in china, were delivered to a jewelry manufacturer in besançon, france. all the boxes were contaminated by mold. because the workers refused to handle these jewelry boxes, the company contacted our laboratory to determine how to deal with the problem. three choices were available: (1) decontaminate the boxes, (2) return the boxes to the chinese manufacturer, or (3) destroy the entire shipment. based on microscopic identification, the culture analysis ...201222702230
a novel glycosylphosphatidylinositol-anchored glycoside hydrolase from ustilago esculenta functions in β-1,3-glucan degradation.a glycoside hydrolase responsible for laminarin degradation was partially purified to homogeneity from a ustilago esculenta culture filtrate by weak-cation-exchange, strong-cation-exchange, and size-exclusion chromatography. three proteins in enzymatically active fractions were digested with chymotrypsin followed by liquid chromatography-tandem mass spectrometry (lc/ms/ms) analysis, resulting in the identification of three peptide sequences that shared significant similarity to a putative β-1,3- ...201222685137
transcripts of a heterologous gene encoding mite allergen der f 7 are stabilized by codon optimization in aspergillus oryzae.we have previously demonstrated that transcripts of an at-biased heterologous gene encoding mite allergen der f 7 from dermatophagoides farinae were polyadenylated prematurely within the coding region when native cdna was expressed in aspergillus oryzae, and that this premature polyadenylation was prevented by the codon optimization of the der f 7 gene, resulting in increased steady-state mrna levels. in this study, we tested the stability of transcription products derived from expression constr ...201222644529
efficient heterologous expression and secretion in aspergillus oryzae of a llama variable heavy-chain antibody fragment v(hh) against egfr.we have constructed a filamentous fungus aspergillus oryzae that secretes a llama variable heavy-chain antibody fragment (v(hh)) that binds specifically to epidermal growth factor receptor (egfr) in a culture medium. a major improvement in yield was achieved by fusing the v(hh) with a taka-amylase a signal sequence (staa) and a segment of 28 amino acids from the n-terminal region of rhizopus oryzae lipase (n28). the yields of secreted, immunologically active anti-egfr v(hh) reached 73.8 mg/1 in ...201222644525
comparative proteome analysis of aspergillus oryzae 3.042 and a. oryzae 100-8 strains: towards the production of different soy sauce flavors.aspergillus oryzae plays a central role in soybean fermentation, particularly in its contribution to the flavor of soy sauce. we present a comparative assessment of the intracellular differences between wild-type strain 3.042 and mutant strain a100-8, at the proteome level. 522 different protein spots were identified by maldi-tof ms, with 134 spots being confirmed by maldi-tof ms/ms. of these, 451 were differentially expressed proteins (deps). there was at least a two-fold increase for 288 spots ...201222634401
a heptaketide naphthaldehyde produced by a polyketide synthase from nectria haematococca.bostrycoidin and fusarubin are biologically active fungal polyketides produced by nectria haematococca. this azaanthraquinone and naphthoquinone are thought to be biosynthesized via formation of a c(14) heptaketide aldehyde as a common key intermediate. a blast search against the genome of n. haematococca revealed one candidate gene (nechadraft_101778, nhpks1), which encodes a multi-domain polyketide synthase (pks) with a thiol reductase (tr) domain that would facilitate the reductive release of ...201222633689
gene cloning, purification, and characterization of a novel peptidoglutaminase-asparaginase from aspergillus sojae.glutaminase is an enzyme that catalyzes the hydrolysis of l-glutamine to l-glutamate, and it plays an important role in the production of fermented foods by enhancing the umami taste. by using the genome sequence and expressed sequence tag data available for aspergillus oryzae rib40, we cloned a novel glutaminase gene (asgaha) from aspergillus sojae, which was similar to a previously described gene encoding a salt-tolerant, thermostable glutaminase of cryptococcus nodaensis (cngaha). the structu ...201222610430
changes in selected physical property and enzyme activity of rice and barley koji during fermentation and storage.koji are solid-state fermentation products made by inoculating steamed grains with the spores of fungi, particularly aspergillus spp. this research was undertaken to identify the fermentation and storage conditions optimal for the production and maintenance of selected hydrolytic enzymes, such as α-amlyase and protease, in koji. steamed rice and barley were inoculated with 2 × 10 ¹¹ aspergillus oryzae spores per kilogram of grains and fermented for 118 h in a growth chamber at 28 to 32 °c with c ...201222583119
two new steroidal saponins from the biotransformation product of the rhizomes of dioscorea nipponica.two new steroidal saponins, dioscins e (1) and f (2), along with nine known steroidal saponins, were isolated from the biotransformation product of the rhizomes of dioscorea nipponica using aspergillus oryzae. the structures of new compounds were established as 25(r)-spirost-5-en-21β-methyl-3β-ol-3-o-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranoside (1) and (25r)-spirost-5-en-3β-ol-7-one 3-o-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranoside (2) by detailed spectroscopic analyses including 1d and 2d ...201222582713
the evolution of three types of indoleamine 2,3 dioxygenases in fungi with distinct molecular and biochemical characteristics.indoleamine 2,3-dioxygenase (ido) is a tryptophan-degrading enzyme and known as a mammalian immunosuppressive molecule. in fungi, the primary role of ido is to supply nicotinamide adenine dinucleotide (nad(+)) via the kynurenine pathway. we previously reported that the koji-mold, aspergillus oryzae has two ido genes, idoα and idoβ. in the present study, we found that a. oryzae also has the third ido, idoγ. these three-types of idos are widely distributed among the pezizomycotina fungi, although ...201222564706
meju, unsalted soybeans fermented with bacillus subtilis and aspergilus oryzae, potentiates insulinotropic actions and improves hepatic insulin sensitivity in diabetic rats. 201222550941
lect-hepa, a glyco-marker derived from multiple lectins, as a predictor of liver fibrosis in chronic hepatitis c patients.assessment of liver fibrosis in patients with chronic hepatitis c (chc) is critical for predicting disease progression and determining future antiviral therapy. lect-hepa, a new glyco-marker derived from fibrosis-related glyco-alteration of serum alpha 1-acid glycoprotein, was used to differentiate cirrhosis from chronic hepatitis in a single-center study. herein, we aimed to validate this new glyco-marker for estimating liver fibrosis in a multicenter study. overall, 183 chc patients were recru ...201222535703
evaluation of enzyme activity and fiber content of soybean cotyledon fiber and distiller's dried grains with solubles by solid state fermentation.to increase the value of coproducts from corn ethanol fermentation and soybean aqueous processing, distiller's dried grains with solubles (ddgs) and soybean cotyledon fiber were used as the substrates for solid state fermentation (ssf) to improve feed digestibility. aspergillus oryzae, trichoderma reesei, and phanerochaete chrysosporium were chosen as they produce desirable enzymes and are widely used in ssf for feed. the results showed that the cellulase and xylanase activities were significant ...201222528656
kinetic modeling of fructooligosaccharide production using aspergillus oryzae n74.in this study, the kinetic for the bioconversion of sucrose to fructooligosaccharides (fos) by free cells of aspergillus oryzae n74 was modeled. in addition, the effect of immobilized glucose isomerase (igi) on fos production yield was evaluated and considered in the kinetic model. the selected kinetic models were based on a proposed reaction mechanism described by elementary rate equations and modified michaelis-menten kinetic equations. the use of igi allowed to increase the fos production yie ...201222528647
synthesis of β-d-fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-d-glucopyranoside (n-acetylsucrosamine) using β-fructofuranosidase-containing aspergillus oryzae mycelia as a whole-cell catalyst.using soft granules consisting of celite 535 and dried aspergillus oryzae nbrc100959 mycelia containing β-fructofuranosidase as a whole-cell catalyst, n-acetylsucrosamine [β-d-fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-d-glucopyranoside] was produced from sucrose and 2-acetamido-2-deoxy-d-glucose by enzymatic transfructosylation. the isolated yield of n-acetylsucrosamine from the reaction mixture was 22.1% (from sucrose). the result of n-terminal amino acid sequence analysis indicated that the ...201222525099
genetic, molecular, and biochemical basis of fungal tropolone biosynthesis.a gene cluster encoding the biosynthesis of the fungal tropolone stipitatic acid was discovered in talaromyces stipitatus (penicillium stipitatum) and investigated by targeted gene knockout. a minimum of three genes are required to form the tropolone nucleus: tropa encodes a nonreducing polyketide synthase which releases 3-methylorcinaldehyde; tropb encodes a fad-dependent monooxygenase which dearomatizes 3-methylorcinaldehyde via hydroxylation at c-3; and tropc encodes a non-heme fe(ii)-depende ...201222508998
modified cre-loxp recombination in aspergillus oryzae by direct introduction of cre recombinase for marker gene rescue.marker rescue is an important molecular technique that enables sequential gene deletions. the cre-loxp recombination system has been used for marker gene rescue in various organisms, including aspergilli. however, this system requires many time-consuming steps, including construction of a cre expression plasmid, introduction of the plasmid, and cre expression in the transformant. to circumvent this laborious process, we investigated a method wherein cre could be directly introduced into aspergil ...201222504800
enhancing the value of nitrogen from rapeseed meal for microbial oil production.rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (fan): commercial enzymes (ces) (2.7 mg g⁻¹ dry meal), liquid state fungal pre-treatment (lsf) using aspergillus oryzae (4.6 mg g⁻¹), liquid state fungal pre-treatment followed by fungal autoly ...201222500902
effect of a novel phytase on growth performance, apparent metabolizable energy, and the availability of minerals and amino acids in a low-phosphorus corn-soybean meal diet for broilers.the addition of microbial phytase to diets for broiler chickens has been shown to improve the availability of phytate p, total p, some other minerals, and amino acids. in this study, the effect of a novel microbial phytase expressed by synthetic genes in aspergillus oryzae on amino acid and mineral availability was assessed. phytase was incorporated (1,000 and 2,000 u/kg) into low-p corn-soybean meal-based diets for broilers. broilers received the experimental diets for 3 wk, and excreta were co ...201222499869
cloning, expression, and characterization of a cellobiose dehydrogenase from thielavia terrestris induced under cellulose growth conditions.the enzyme cellobiose dehydrogenase (cdh) is of considerable interest, not only for its biotechnological applications, but also its potential biological role in lignocellulosic biomass breakdown. the enzyme catalyzes the oxidation of cellobiose and other cellodextrins, utilizing a variety of one- and two-electron acceptors, although the electron acceptor employed in nature is still unknown. in this study we show that a cdh is present in the secretome of the thermophilic ascomycete thielavia terr ...201222484439
monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis.soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ...201222475947
bioremediation of direct dyes in simulated textile effluents by a paramorphogenic form of aspergillus oryzae.azo dyes are extensively used for coloring textiles, paper, food, leather, drinks, pharmaceutical products, cosmetics and inks. the textile industry consumes the largest amount of azo dyes, and it is estimated that approximately 10-15% of dyes used for coloring textiles may be lost in waste streams. almost all azo dyes are synthetic and resist biodegradation, however, they can readily be reduced by a number of chemical and biological reducing systems. biological treatment has advantages over phy ...201222466598
overexpressing transcriptional regulator in aspergillus oryzae activates a silent biosynthetic pathway to produce a novel polyketide.fungal genomes carry many gene clusters seemingly capable of natural product biosynthesis, yet most clusters remain silent. this places a major constraint on the conventional approach of cloning these genes in more amenable heterologous host for the natural product biosynthesis. one way to overcome this difficulty is to activate the silent gene clusters within the context of the target fungus. here, we successfully activated a silent polyketide biosynthetic gene cluster in aspergillus oryzae by ...201222447538
antimicrobial activity of saponin-rich fraction from camellia oleifera cake and its effect on cell viability of mouse macrophage raw 264.7.as a by-product of oil production, several million tons of camellia oleifera cake is discarded every year in china. the aim of this study was to evaluate the antimicrobial activity of a saponin-rich fraction isolated from c. oleifera cake and investigate its effect on the cell viability of raw 264.7, a macrophage-like cell line present in almost all mouse tissues.201222430639
a new zn(ii)(2)cys(6)-type transcription factor bglr regulates β-glucosidase expression in trichoderma reesei.bglr (pi: 52368, beta-glucosidaseregulator) was identified as a new transcription factor that up-regulates expression of specific genes encoding β-glucosidases. based on a comparative genomic analysis to verify snps between trichoderma reesei mutant pc-3-7 and its parent kdg-12, 19 were confirmed. one of the snps was found to cause a missense mutation close to the end of the dna-binding region of bglr that turned out to be a zn(ii)(2)cys(6)-type fungal-specific transcription factor. bglr was fou ...201222425594
amperometric glucose biosensor utilizing fad-dependent glucose dehydrogenase immobilized on nanocomposite electrode.amperometric glucose biosensors utilizing commercially available fad-dependent glucose dehydrogenases from two strains of aspergillus species are described. enzymes were immobilized on nanocomposite electrode consisting of multi-walled carbon nanotubes by entrapment between chitosan layers. unlike the common glucose oxidase based biosensor, the presented biosensors appeared to be o(2)-independent. the optimal amount of enzymes, working potential and ph value of working media of the glucose biose ...201222418262
the influence of inoculum composition on selected bioactive and nutritional parameters of grass pea tempeh obtained by mixed-culture fermentation with rhizopus oligosporus and aspergillus oryzae strains.tempeh is a popular indonesian product obtained from legume seeds by solid-state fermentation with rhizopus sp. the aim of this research was to study the effect of simultaneous mixed-culture fermentation of grass pea seeds on selected parameters of products as compared to traditional tempeh. the inoculum contained different ratios of rhizopus oligosporus and aspergillus oryzae spores. the simultaneous fermentation of grass pea seeds with inoculum consisting of 1.2 × 10(6) r. oligosporus and 0.6  ...201222414936
effect of aspergillus oryzae-challenged germination on soybean isoflavone content and antioxidant activity.application of microbial stress to soybean during germination induces the accumulation of phytoalexins, which have many health benefits. in this study, the effects of stress induced by aspergillus oryzae on the phytochemical composition of germinating soybeans were investigated, and their radical scavenging activity was compared with those of ungerminated (us) and germinated (gs) soybeans. additionally, the antioxidant activity of coumestrol, a soybean phytoalexin, against hydrogen peroxide-indu ...201222409158
extract of fermented brown rice induces apoptosis of human colorectal tumor cells by activating mitochondrial pathway.brown rice fermented with aspergillus oryzae, designated as fbra, is a dietary fiber-rich food, and fully appreciated as one of the prebiotics, which are generally considered to be beneficial to the health of the body, because of stimulating the growth and/or the activity of bacteria in the digestive system. to assess the effectiveness of fbra as a functional food, the direct effect of fbra extract on human colorectal tumor cells was examined. the exposure of hct116 cells to fbra extract reduced ...201222389198
biocatalytic conversion of aloeresin a to aloesin.leaf exudates from aloe species, such as the southern african aloe ferox, are used in traditional medicines for both humans and livestock. this includes aloesin, a skin bleaching product that inhibits the synthesis of melanin. aloesin, (a c-glycoside-5-methylchromone) can be released from aloeresin a, an ester of aloesin, through hydrolysis. the objective of the current study was to identify an enzymatic hydrolysis method for converting aloeresin a to aloesin, resulting in increased concentratio ...201222374229
vector-initiated transitive rna interference in the filamentous fungus aspergillus oryzae.rna interference (rnai), modulates gene expression via cleavage of double-stranded rna (dsrna) by dicer, producing 21-25 nucleotide silence-inducing rnas (sirnas). in association with argonaute containing complexes, these sirnas target sequence-specific degradation of the homologous single-stranded messenger rna. in the majority of eukaryotes, degradation occurs within the boundaries of the dsrna target. in arabidopsis thaliana and caenorhabditis elegans, gene silencing can also take place trans ...201222366516
secretome of aspergillus oryzae in shaoxing rice wine koji.shaoxing rice wine is the most famous and representative chinese rice wine. aspergillus oryzae su16 is used in the manufacture of koji, the shaoxing rice wine starter culture. in the current study, a comprehensive analysis of the secretome profile of a. oryzae su16 in shaoxing rice wine koji was performed for the first time. the proteomic analysis for the identification of the secretory proteins was done using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionizat ...201222341915
presence and functionality of mating type genes in the supposedly asexual filamentous fungus aspergillus oryzae.the potential for sexual reproduction in aspergillus oryzae was assessed by investigating the presence and functionality of mat genes. previous genome studies had identified a mat1-1 gene in the reference strain rib40. we now report the existence of a complementary mat1-2 gene and the sequencing of an idiomorphic region from a. oryzae strain ao6. this allowed the development of a pcr diagnostic assay, which detected isolates of the mat1-1 and mat1-2 genotypes among 180 strains assayed, including ...201222327593
analysis of the functions of recombination-related genes in the generation of large chromosomal deletions by loop-out recombination in aspergillus oryzae.loop-out-type recombination is a type of intrachromosomal recombination followed by the excision of a chromosomal region. the detailed mechanism underlying this recombination and the genes involved in loop-out recombination remain unknown. in the present study, we investigated the functions of ku70, ligd, rad52, rad54, and rdh54 in the construction of large chromosomal deletions via loop-out recombination and the effect of the position of the targeted chromosomal region on the efficiency of loop ...201222286092
anti-obesity effect of a standardised ethanol extract from curcuma longa l. fermented with aspergillus oryzae in ob/ob mice and primary mouse adipocytes.we examined the anti-obesity effect of fermented curcuma longa l. (turmeric) standardised ethanol extract (fte) in the c57bl/6j ob/ob mouse model. mice were fed a chow diet containing fte (0, 200, or 500 mg kg⁻¹ body weight) for 9 weeks.201222278718
effects of koji fermented phenolic compounds on the oxidative stability of fish miso.in the present study, aspergillus oryzae-inoculated koji inhibited lipid oxidation in fermented fish paste rich in polyunsaturated fatty acids following a long fermentation period. the fermentation of koji by a. oryzae liberated several bioactive phenolic compounds, including kojic acid and ferulic acid, which were the most abundant. a linear correlation between several phenolic compounds and their bioactive properties, including their radical-scavenging activity, reducing power, metal-chelating ...201222251370
comparative chemistry of aspergillus oryzae (rib40) and a. flavus (nrrl 3357).aspergillus oryzae and a. flavus are important species in industrial biotechnology and food safety and have been some of the first aspergilli to be fully genome sequenced. bioinformatic analysis has revealed 99.5% gene homology between the two species pointing towards a large coherence in the secondary metabolite production. in this study we report on the first comparison of secondary metabolite production between the full genome sequenced strains of a. oryzae (rib40) and a. flavus (nrrl 3357). ...201224957367
kinetics of improved 1,4-alpha-d-glucan glucohydrolase biosynthesis from a newly isolated aspergillus oryzae iib-6 and parameter significance analysis by 2-factorial design.sixteen different mould cultures viz. aspergillus, alternaria, arthroderma, trichoderma, fusarium, penicillium, rhizopus and chochliobolus were isolated from the soil samples of qatar by serial dilution method. the preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar plates. the isolates were then subjected to secondary screening by submerged fermentation (smf). the 1,4-α-d-glucan glucohydrolase (ggh) activ ...201223961361
Effect of steam explosion and microbial fermentation on cellulose and lignin degradation of corn stover.In order to increase nutrient values of corn stover, effects of steam explosion (2.5MPa, 200s) and Aspergillus oryzae (A. oryzae) fermentation on cellulose and lignin degradation were studied. The results showed the contents of cellulose, hemicellulose and lignin in the exploded corn stover were 8.47%, 50.45% and 36.65% lower than that in the untreated one, respectively (P<0.05). The contents of cellulose and hemicellulose in the exploded and fermented corn stover (EFCS) were decreased by 24.36% ...201222104102
[effect of n-terminal disulfide bridge on thermostability of family 11 xylanases].a mesophilic xylanase from aspergillus oryzae, abbreviated to aoxyn11a, belongs to glycoside hydrolase family 11. using aoxyn11a as the parent, the thermotolerant hybrid xylanase, we constructed aex11a by substituting its n-terminus with the corresponding region of a hyperthermostable family 11 xylanase, evxyn11(ts). aoxyn11a- and aex11a-encoding genes were expressed in pichia pastoris gs115 separately, and effects of temperatures on expressed products were determined and compared. the optimum t ...201223593868
optimisation of synthesis of oligosaccharides derived from lactulose (fructosyl-galacto-oligosaccharides) with β-galactosidases of different origin.batch synthesis of fructosyl-galacto-oligosaccharides from lactulose was performed with commercial β-galactosidase preparations from aspergillus oryzae, kluyveromyces lactis and bacillus circulans. the enzyme from a. oryzae produced the highest yield and specific productivity of synthesis, being selected for further studies. optimization of fructosyl-galacto-oligosaccharides synthesis was carried out using response surface methodology, considering temperature and initial sugar concentration as v ...201223497880
the effects of supplementation of a novel bacterial 6-phytase on mineral digestibility and plasma minerals in lactating sows.pigs digest p in plant feedstuffs poorly because pigs do not produce sufficient endogenous phytase to hydrolyze p from phytate (inositol hexaphosphate). supplementation of phytase to diets of piglets and grower-finisher pigs increased digestibility of minerals including p and ca; however, data on phytase efficacy in lactating sows are scarce. therefore, effects of adding a bacterial 6-phytase expressed in a strain of aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerla ...201223365301
the supplementation of low-p diets with microbial 6-phytase expressed in aspergillus oryzae improves p digestibility in sows.two trials were conducted to evaluate a novel microbial 6-phytase expressed in aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerland) in gestating and lactating sows. in the first trial, 24 sows (duroc × landrace; 223 kg bw) were offered, at 16 d of gestation, a low-p control diet (formulated to provide 4.0 g total p/kg; 1.5 g digestible p/kg) supplemented with 0, 500, or 1000 phytase activity (fyt)/kg of phytase. two weeks later, fresh feces were sampled from all sow ...201223365297
the supplementation of low-p diets with microbial 6-phytase expressed in aspergillus oryzae increases p and ca digestibility in growing pigs.a trial was conducted to evaluate the dose response of a novel microbial 6-phytase expressed in aspergillus oryzae (ronozyme hiphos; dsm nutritional products, basel, switzerland) in pigs. forty-eight individually housed pigs (landrace × pietrain; 52 kg bw; 24 males and 24 females) were distributed among 6 experimental treatments consisting of a low-p diet (3.5 g p/kg; 1.1 g digestible p/kg), which was supplemented with 500, 1000, 2000, or 4000 units of phytase activity/kg, and a standard-p diet ...201223365288
synthetic dye decolorization by three sources of fungal laccase.decolorization of six synthetic dyes using three sources of fungal laccase with the origin of aspergillus oryzae, trametes versicolor, and paraconiothyrium variabile was investigated. among them, the enzyme from p. variabile was the most efficient which decolorized bromophenol blue (100%), commassie brilliant blue (91%), panseu-s (56%), rimazol brilliant blue r (rbbr; 47%), congo red (18.5%), and methylene blue (21.3%) after 3 h incubation in presence of hydroxybenzotriazole (hbt; 5 mm) as the l ...201223369690
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