Publications
Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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catalytic conversion of sugarcane bagasse to cellulosic ethanol: tio2 coupled nanocellulose as an effective hydrolysis enhancer. | the present study deals with the production of cellulosic ethanol from bagasse using the synthesized tio2 coupled nanocellulose (nc-tio2) as catalyst. aspergillus nidulans ajsu04 cellulase was used for the hydrolysis of bagasse. nc-tio2 at various concentrations was added to bagasse in order to enhance the yield of reducing sugars. complex interaction between cellulase, bagasse, nc-tio2 and the reaction environment is thoroughly studied. a mathematical model was developed to describe the hydroly ... | 2016 | 26572403 |
design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: optimization and synergism studies. | lignocellulosic materials represent a very important and promising source of renewable biomass. in order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of trichoderma harzianum ioc 3844, penicillium funiculosum atcc 11797, and aspergillus niger atcc 1004, result ... | 2016 | 27254751 |
secretome data from trichoderma reesei and aspergillus niger cultivated in submerged and sequential fermentation methods. | the cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. the proteomic analysis data presented here provide critical information to compare proteins secreted by trichoderma reesei and aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. the proteins were organized according to the families described in cazy ... | 2016 | 27419196 |
secretome analysis of trichoderma reesei and aspergillus niger cultivated by submerged and sequential fermentation processes: enzyme production for sugarcane bagasse hydrolysis. | cellulases and hemicellulases from trichoderma reesei and aspergillus niger have been shown to be powerful enzymes for biomass conversion to sugars, but the production costs are still relatively high for commercial application. the choice of an effective microbial cultivation process employed for enzyme production is important, since it may affect titers and the profile of protein secretion. we used proteomic analysis to characterize the secretome of t. reesei and a. niger cultivated in submerge ... | 2016 | 27241292 |
addition of metal ions to a (hemi)cellulolytic enzymatic cocktail produced in-house improves its activity, thermostability, and efficiency in the saccharification of pretreated sugarcane bagasse. | high activity and stability are essential for (hemi)cellulolytic enzymes used in biomass conversion, while non-productive binding of cellulases to lignin reduces saccharification efficiency and needs to be avoided. one potential strategy is the addition of inexpensive metal ions. this paper describes the influence of divalent metal ions on the activity, thermostability, and saccharification efficiency of (hemi)cellulolytic enzymes produced in-house by aspergillus niger under solid-state fermenta ... | 2016 | 26709004 |
characterization of the cellulolytic secretome of trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin. | this study demonstrates the production of an active enzyme cocktail produced by growing trichoderma harzianum on sugarcane bagasse. the component enzymes were identified by lcms-ms. glycosyl hydrolases were the most abundant class of proteins, representing 67% of total secreted protein. other carbohydrate active enzymes involved in cell wall deconstruction included lytic polysaccharide mono-oxygenases (aa9), carbohydrate-binding modules, carbohydrate esterases and swollenin, all present at level ... | 2016 | 26697775 |
s-triazine degrading bacterial isolate arthrobacter sp. ak-yn10, a candidate for bioaugmentation of atrazine contaminated soil. | the arthrobacter sp. strain ak-yn10 is an s-triazine pesticide degrading bacterium isolated from a sugarcane field in central india with history of repeated atrazine use. ak-yn10 was shown to degrade 99 % of atrazine in 30 h from media supplemented with 1000 mg l(-1) of the herbicide. draft genome sequencing revealed similarity to pao1, tc1, and tc2 catabolic plasmids of the arthrobacter taxon. plasmid profiling analyses revealed the presence of four catabolic plasmids. the trzn, atzb, and atzc ... | 2016 | 26403923 |
anaerobic biological treatment of vinasse for environmental compliance and methane production. | the energy crisis resulted in increasing awareness that alternative sources of energy should be considered. during this time, brazil implemented ethanol production from sugarcane as biofuel. however, during this process, large amounts of residues are generated, such as vinasse. this residue can be treated anaerobically to generate methane as a source of bioenergy with the use of sequencing batch reactors operated with immobilized biomass (ansbbr). in this work, tests were conducted in an ansbbr ... | 2016 | 26400496 |
effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities. | this work shows both cellulases and hemicellulases are inhibited and deactivated by water-soluble and acetone extracted phenolics from sugarcane bagasse pretreated at 10% (w/v) for 30 min in liquid hot water at 180 or 200°c. the dissolved phenolics in vacuum filtrate increased from 1.4 to 2.4 g/l as temperature increased from 180 to 200°c. the suppression of cellulose and hemicellulose hydrolysis by phenolics is dominated by deactivation of the β-glucosidase or β-xylosidase components of cellula ... | 2016 | 26364828 |
enzymatic hydrolysis of pretreated sugarcane straw: kinetic study and semi-mechanistic modeling. | although there are already commercial-scale productions of second generation (2g) ethanol, focusing efforts on process optimization can be of key importance to make the production cost-effective in large scale. in this scenario, mathematical models may be useful in design, scale-up, optimization, and control of bioreactors. for this reason, the aim of this work was to study the kinetics of the enzymatic hydrolysis of cellulose from sugarcane straw. experiments using hydrothermally pretreated sug ... | 2016 | 26701144 |
landscape, environmental and social predictors of hantavirus risk in são paulo, brazil. | hantavirus pulmonary syndrome (hps) is a disease caused by hantavirus, which are negative-sense rna viruses in the family bunyaviridae that are highly virulent to humans. numerous factors modify risk of hantavirus transmission and consequent hps risk. human-driven landscape change can foster transmission risk by increasing numbers of habitat generalist rodent species that serve as the principal reservoir host. climate can also affect rodent population dynamics and hantavirus survival, and a numb ... | 2016 | 27780250 |
optimization of substrate preparation for oyster mushroom (pleurotus ostreatus) cultivation by studying different raw materials and substrate preparation conditions (composting: phases i and ii). | in recent years, oyster mushroom (pleurotus ostreatus) has become one of the most cultivated mushrooms in the world, mainly in brazil. among many factors involved in a mushroom production, substrate preparation is the most critical step, which can be influenced by composting management techniques. looking forward to optimizing the substrate preparation process, were tested different composting conditions (7 and 14 days of composting with or without conditioning), potential raw materials (decumbe ... | 2016 | 27696289 |
phosphate solubilization potential of rhizosphere fungi isolated from plants in jimma zone, southwest ethiopia. | phosphorus (p) is one of the major bioelements limiting agricultural production. phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. the present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya (pvk) medium. a total of 359 fungal isolates were obtained from 150 rhizosphere soil samples of haricot bean, faba bean, cabbage, tomato, and suga ... | 2016 | 27688771 |
soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse. | addition of surfactants, polymers, and non-catalytic proteins can improve the enzymatic hydrolysis of lignocellulosic materials by blocking the exposed lignin surfaces, but involves extra expense. here, soybean protein, one of the cheapest proteins available, was evaluated as an alternative additive for the enzymatic hydrolysis of pretreated sugarcane bagasse. the effect of the enzyme source was investigated using enzymatic cocktails from a. niger and t. reesei cultivated under solid-state, subm ... | 2016 | 27639236 |
liming in the sugarcane burnt system and the green harvest practice affect soil bacterial community in northeastern são paulo, brazil. | here we show that both liming the burnt sugarcane and the green harvest practice alter bacterial community structure, diversity and composition in sugarcane fields in northeastern são paulo state, brazil. terminal restriction fragment length polymorphism fingerprinting and 16s rrna gene cloning and sequencing were used to analyze changes in soil bacterial communities. the field experiment consisted of sugarcane-cultivated soils under different regimes: green sugarcane (gs), burnt sugarcane (bs), ... | 2016 | 27629424 |
phenolic compounds and anticancer activity of commercial sugarcane cultivated in brazil. | the cultivation of sugarcane hybrids (x saccharum officinarum l.) is an important revenue source for the brazilian economy. herein it is reported the evaluation of the cytotoxic activity of mid-polarity sugarcane extracts against human cancer cell lines, as well as the isolation of steroids sitosterol, stigmasterol and campesterol, phenolic acids p-hydroxybenzoic, p-hydroxycinnamic, vanillic and ferulic acid, terpenoids α-tocopherol and β-carotene and a novel substance in sugarcane, the flavonoi ... | 2016 | 27598841 |
synthesis of cellulose acetate and carboxymethylcellulose from sugarcane straw. | sugarcane straw (scs) is a raw material with high potential for production of cellulose derivatives due to its morphology and structure. the proposal of this work was to synthesize cellulose acetate (ca) and carboxymethylcellulose (cmc) from sugarcane straw cellulose, and applied the ca in the preparation of a membrane. the cellulose extraction was carried out in four steps. firstly, scs was treated with h2so4 (10% v/v) followed by naoh (5% w/v) treatment. subsequently, a chelating process was p ... | 2016 | 27516319 |
effects of genotype, wilting, and additives on the nutritive value and fermentation of bermudagrass silage. | bermudagrass is the main warm-season grass species used for livestock production in the southeastern united states; however, when it is ensiled, the silage fermentation parameters are often less than desirable. the objective of this study was to investigate the effects of management practices on the nutritive value and fermentation characteristics of bermudagrass silage. in exp. 1, treatments were the factorial combinations of 2 bermudagrass genotypes, 'jiggs' () and 'tifton 85' ( sp.), 4 additi ... | 2016 | 27482693 |
nitrous oxide emission related to ammonia-oxidizing bacteria and mitigation options from n fertilization in a tropical soil. | nitrous oxide (n2o) from nitrogen fertilizers applied to sugarcane has high environmental impact on ethanol production. this study aimed to determine the main microbial processes responsible for the n2o emissions from soil fertilized with different n sources, to identify options to mitigate n2o emissions, and to determine the impacts of the n sources on the soil microbiome. in a field experiment, nitrogen was applied as calcium nitrate, urea, urea with dicyandiamide or 3,4 dimethylpyrazone phosp ... | 2016 | 27460335 |
characterization of sugarcane (saccharum spp.) leaf senescence: implications for biofuel production. | second-generation ethanol (2g-bioethanol) uses lignocellulosic feedstocks for ethanol production. sugarcane is one among the most suitable crops for biofuel production. its juice is extracted for sugar production, while sugarcane bagasse, straw, and senescing leaves are considered industrial waste. senescence is the age-dependent deterioration of plant cells, ultimately leading to cell death and completion of the plant life cycle. because senescing leaves may also be used for biofuel production, ... | 2016 | 27453728 |
a new genus and two new species of soldier fly (diptera: stratiomyidae: chiromyzinae) from australia, one found infesting sugarcane in central queensland. | metridius nov. gen. and types species m. robertsoni nov. sp. with winged males and apterous females is described from adults and larvae found infesting sugarcane stools from near mackay, central queensland. a second new species, m. mcalpinei nov. sp., based only on males from near sydney, new south wales is also described. notes on the biology of both species and an identification key to the genera of the subfamily chiromyzinae and to the species are also given. | 2016 | 27394475 |
unlocking the bacterial and fungal communities assemblages of sugarcane microbiome. | plant microbiome and its manipulation herald a new era for plant biotechnology with the potential to benefit sustainable crop production. however, studies evaluating the diversity, structure and impact of the microbiota in economic important crops are still rare. here we describe a comprehensive inventory of the structure and assemblage of the bacterial and fungal communities associated with sugarcane. our analysis identified 23,811 bacterial otus and an unexpected 11,727 fungal otus inhabiting ... | 2016 | 27358031 |
treatment of sugarcane vinasse by combination of coagulation/flocculation and fenton's oxidation. | the efficiency of individual and integrated processes applied to organic matter reduction and biodegradability improvement of a biodigested sugarcane vinasse wastewater was assessed. strategies considered were fenton's oxidation (strategy 1), coagulation/flocculation (strategy 2) and the combination of both processes (coagulation/flocculation followed by fenton's reaction) - strategy 3. it was found that fenton's oxidation per se allowed reducing the organic matter, increasing the wastewater bio ... | 2016 | 27353374 |
genome-wide association mapping of quantitative traits in a breeding population of sugarcane. | molecular markers associated with relevant agronomic traits could significantly reduce the time and cost involved in developing new sugarcane varieties. previous sugarcane genome-wide association analyses (gwas) have found few molecular markers associated with relevant traits at plant-cane stage. the aim of this study was to establish an appropriate gwas to find molecular markers associated with yield related traits consistent across harvesting seasons in a breeding population. sugarcane clones ... | 2016 | 27342657 |
effect of ph, temperature, and chemicals on the endoglucanases and β-glucosidases from the thermophilic fungus myceliophthora heterothallica f.2.1.4. obtained by solid-state and submerged cultivation. | this work reports endoglucanase and beta-glucosidase production by the thermophilic fungus myceliophthora heterothallica in solid-state (ssc) and submerged (smc) cultivation. wheat bran and sugarcane bagasse were used for ssc and cardboard for smc. highest endoglucanase production in ssc occurred after 192 hours: 1,170.6 ± 0.8 u/g, and in smc after 168 hours: 2,642 ± 561 u/g. the endoglucanases and beta-glucosidases produced by both cultivation systems showed slight differences concerning their ... | 2016 | 27242927 |
exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. | glycoside hydrolases (ghs) and accessory proteins are key components for efficient and cost-effective enzymatic hydrolysis of polysaccharides in modern, biochemically based biorefineries. currently, commercialized ghs and accessory proteins are produced by ascomycetes. however, the role of wood decay basidiomycetes proteins in biomass saccharification has not been extensively pursued. wood decay fungi degrade polysaccharides in highly lignified tissues in natural environments, and are a promisin ... | 2016 | 27222665 |
determination of pesticides in sugarcane juice employing microextraction by packed sorbent followed by gas chromatography and mass spectrometry. | this paper describes the development of a method for the determination of six pesticides (tebuthiuron, carbofuran, atrazine, metribuzine, ametryn, and bifenthrin) in sugarcane juice using microextraction by packed sorbent as the extraction technique. the extraction steps were optimized by factorial design, being the variables ph, ionic strength, desorption solvent and solvent volume optimized for comparisons among sorbent materials. among the evaluated materials c18-chromabond(®) showed better e ... | 2016 | 27219489 |
air pollutants and hospital admission due to pneumonia in children: a time series analysis. | the aim of this study was to estimate the association between expo sure to air pollutants and hospitalization for pneumonia among children in a medium-sized city located in the sugar cane plantation region of são paulo state. | 2016 | 27167545 |
tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. | grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. however, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. the varied tissue-specific distribution of cell wall components adds ... | 2016 | 27148403 |
loss of soil (macro)fauna due to the expansion of brazilian sugarcane acreage. | land use changes (luc) from pasture to sugarcane (saccharum spp.) crop are expected to add 6.4mha of new sugarcane land by 2021 in the brazilian cerrado and atlantic forest biomes. we assessed the effects of these luc on the abundance and community structure of animals that inhabit soils belowground through a field survey using chronosequences of land uses comprising native vegetation, pasture, and sugarcane along a 1000-km-long transect across these two major tropical biomes in brazil. macrofau ... | 2016 | 27135579 |
boosting tag accumulation with improved biodiesel production from novel oleaginous microalgae scenedesmus sp. iitrind2 utilizing waste sugarcane bagasse aqueous extract (sbae). | this investigation utilized sugarcane bagasse aqueous extract (sbae), a nontoxic, cost-effective medium to boost triacylglycerol (tag) accumulation in novel fresh water microalgal isolate scenedesmus sp. iitrind2. maximum lipid productivity of 112 ± 5.2 mg/l/day was recorded in microalgae grown in sbae compared to modified bbm (26 ± 3 %). carotenoid to chlorophyll ratio was 12.5 ± 2 % higher than in photoautotrophic control, indicating an increase in photosystem ii activity, thereby increasing g ... | 2016 | 27093970 |
raoultella sp. strain l03 fixes n2 in association with micropropagated sugarcane plants. | n2 -fixing bacteria belonging to the genus raoultella of the family enterobacteriaceae are widely associated with plants. raoultella sp. strain l03 was isolated from surface-sterilized sugarcane roots. in this study, we inoculated the strain l03 to microbe-free micropropagated plantlets of the main sugarcane cultivar roc22 grown in guangxi, china and determined n2 -fixation and association between strain l03 and sugarcane plants. inoculation of strain l03 increased plant biomass, total n, n conc ... | 2016 | 27059698 |
thermal decomposition of sugarcane straw, kinetics and heat of reaction in synthetic air. | the aim of this work was to analyze the thermal decomposition, kinetics and heat of reaction of sugarcane straw in synthetic air by thermogravimetry (tg) and differential scanning calorimetry (dsc). the tg and dsc experiments were carried out using heating rates of 2.5°c/min, 5°c/min, and 10°c/min, and particle diameter of 0.250mm. in the study of the smoldering reaction were identified three consecutive stages, drying, oxidative pyrolysis, and combustion. thus, the kinetic pathway was composed ... | 2016 | 27019126 |
exogenous nitric oxide improves sugarcane growth and photosynthesis under water deficit. | nitric oxide (no)-mediated redox signaling plays a role in alleviating the negative impact of water stress in sugarcane plants by improving root growth and photosynthesis. drought is an environmental limitation affecting sugarcane growth and yield. the redox-active molecule nitric oxide (no) is known to modulate plant responses to stressful conditions. no may react with glutathione (gsh) to form s-nitrosoglutathione (gsno), which is considered the main reservoir of no in cells. here, we investig ... | 2016 | 27002974 |
cobalt internal standard for ni to assist the simultaneous determination of mo and ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry employing direct solid sample analysis. | a new method is proposed for the simultaneous determination of mo and ni in plant materials by high-resolution continuum source graphite furnace atomic absorption spectrometry (hr-cs gfaas), employing direct solid sample analysis (dss) and internal standardization (is). cobalt was used as internal standard to minimize matrix effects during ni determinations, enabling the use of aqueous standards for calibration. correlation coefficients for the calibration curves were typically better than 0.993 ... | 2016 | 26992542 |
production of cellulosic ethanol from sugarcane bagasse by steam explosion: effect of extractives content, acid catalysis and different fermentation technologies. | the production of cellulosic ethanol was carried out using samples of native (ncb) and ethanol-extracted (eecb) sugarcane bagasse. autohydrolysis (ah) exhibited the best glucose recovery from both samples, compared to the use of both h3po4 and h2so4 catalysis at the same pretreatment time and temperature. all water-insoluble steam-exploded materials (seb-wi) resulted in high glucose yields by enzymatic hydrolysis. shf (separate hydrolysis and fermentation) gave ethanol yields higher than those o ... | 2016 | 26943936 |
estimating the opportunity costs of environmental conservation in the feijão river watershed (são carlos-sp, brazil). | the objective of this study was to infer the opportunity cost of land use of the feijão river watershed (são carlos-sp, brazil), in order to estimate the financial resources necessary to compensate landowners willing to convert their production areas into areas of environmental preservation. net values were estimated by calculating the annual value and the net present value of each activity. the area used for agricultural production was estimated using the land cover map of the watershed. the st ... | 2016 | 26909620 |
concentration of cu, zn, cr, ni, cd, and pb in soil, sugarcane leaf and juice: residual effect of sewage sludge and organic compost application. | many researchers have evaluated the effects of successive applications of sewage sludge (ss) on soil plant-systems, but most have not taken into account the residual effect of organic matter remaining from prior applications. furthermore, few studies have been carried out to compare the effects of the agricultural use of ss and sewage sludge compost (ssc). therefore, we evaluated the residual effect of ss and ssc on the heavy metal concentrations in soil and in sugarcane (saccharum spp.) leaves ... | 2016 | 26879984 |
genome organization and host range of a brazilian isolate of johnsongrass mosaic virus. | this work reports the complete genome sequence, production of a polyclonal antiserum, and host range of a brazilian strain of johnsongrass mosaic virus (jgmv) found infecting panicum maximum in the state of são paulo, brazil. the complete genome sequence of this potyvirus, comprising 9874 nucleotides, showed 82 % amino acid sequence identity in the polyprotein to that of an isolate of jgmv from australia. the experimental host range of this virus included mainly fodder species. cultivated specie ... | 2016 | 26869279 |
estimating the opportunity costs of environmental conservation in the feijão river watershed (são carlos-sp, brazil). | the objective of this study was to infer the opportunity cost of land use of the feijão river watershed (são carlos-sp, brazil), in order to estimate the financial resources necessary to compensate landowners willing to convert their production areas into areas of environmental preservation. net values were estimated by calculating the annual value and the net present value of each activity. the area used for agricultural production was estimated using the land cover map of the watershed. the st ... | 2016 | 26840574 |
molecular characterization of soil organic matter from native vegetation-pasture-sugarcane transitions in brazil. | replacing pastures (pa) with sugarcane (sg) has been deemed an agronomically feasible strategy for sugarcane expansion in brazil. however, there are some uncertainties about the environmental impacts regarding this land use change (luc), mainly related to soil organic matter (som), a key factor of environmental sustainability of brazilian ethanol. luc-related losses of som can overcome the c savings from biofuels. the molecular composition of som was evaluated to understand the c dynamics regard ... | 2016 | 26828621 |
cell wall proteome of sugarcane stems: comparison of a destructive and a non-destructive extraction method showed differences in glycoside hydrolases and peroxidases. | sugarcane has been used as the main crop for ethanol production for more than 40 years in brazil. recently, the production of bioethanol from bagasse and straw, also called second generation (2g) ethanol, became a reality with the first commercial plants started in the usa and brazil. however, the industrial processes still need to be improved to generate a low cost fuel. one possibility is the remodeling of cell walls, by means of genetic improvement or transgenesis, in order to make the bagass ... | 2016 | 26754199 |
monitoring of the cellulosic ethanol fermentation process by near-infrared spectroscopy. | rapid, efficient, and low-cost technologies for monitoring the fermentation process during second generation (2g) or cellulosic ethanol production are essential for the successful implementation of this process at the commercial scale. here, the use of near-infrared (nir) spectroscopy associated with partial least squares (pls) regression was investigated as a tool for monitoring the production of 2g ethanol from lignocellulosic sugarcane residues including bagasse, straw, and tops. the spectral ... | 2016 | 26748047 |
comparison of submerged and solid state pretreatment of sugarcane bagasse by pandoraea sp. istkb: enzymatic and structural analysis. | pretreatment of sugarcane bagasse by pandoraea sp. istkb was evaluated under submerged (smf) and solid state (ssf) culture conditions. number of bacteria was 2.7 times higher in smf as compared with ssf. enzymes produced under smf were xylanase, lip, mnp and laccase. in ssf xylanase and laccase were detected. cmcase, fpase and β-glucosidase were not detected. delignification was highest in smf with 19.94% and 10.43% removal of hemicelluloses and lignin, respectively. ftir analysis suggested the ... | 2016 | 26720135 |
alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. | sugarcane bagasse is a by-product from the sugar and ethanol industry which contains approximately 70 % of its dry mass composed by polysaccharides. to convert these polysaccharides into fuel ethanol it is necessary a pretreatment step to increase the enzymatic digestibility of the recalcitrant raw material. in this work, sugarcane bagasse was pretreated by an alkaline-sulfite chemithermomechanical process for increasing its enzymatic digestibility. na2so3 and naoh ratios were fixed at 2:1, and ... | 2016 | 26718203 |
environmental indicators for sustainability: a strategic analysis for the sugarcane ethanol context in brazil. | sugarcane ethanol is considered a renewable energy source and has emerged as a potential alternative to reduce dependency on fossil fuels, particularly in brazil. however, there are some questions about how sustainable this energy source is, given the impacts from its production and use on a larger scale. to understand and achieve sustainability, it is essential to build tools that can assess an integrated conception and help decision-makers to establish public policies for a sustainable develop ... | 2016 | 26153435 |
controlled release of drugs from cellulose acetate matrices produced from sugarcane bagasse: monitoring by square-wave voltammetry. | in this paper, cellulose triacetate (cta) was produced from sugarcane bagasse and used as matrices for controlled release of paracetamol. symmetric and asymmetric membranes were obtained by formulations of cta/dichloromethane/drug and cta/dichloromethane/water/drug, respectively, and they were characterized by scanning electron microscopy (sem) and differential scanning calorimetry (dsc). different morphologies of membranes were observed by sem, and the incorporation of paracetamol was confirmed ... | 2016 | 26596497 |
chemical data as markers of the geographical origins of sugarcane spirits. | in an attempt to classify sugarcane spirits according to their geographic region of origin, chemical data for 24 analytes were evaluated in 50 cachaças produced using a similar procedure in selected regions of brazil: são paulo - sp (15), minas gerais - mg (11), rio de janeiro - rj (11), paraiba -pb (9), and ceará - ce (4). multivariate analysis was applied to the analytical results, and the predictive abilities of different classification methods were evaluated. principal component analysis ide ... | 2016 | 26593483 |
health risks due to pre-harvesting sugarcane burning in são paulo state, brazil. | after 2003, a new period of expansion of the sugarcane culture began in brazil. pre-harvesting burning of sugarcane straw is an agricultural practice that, despite the nuisance for the population and pollution generated, still persisted in over 70% of the municipalities of são paulo state in 2010. in order to study the distribution of this risk factor, an ecological epidemiological study was conducted associating the rates of deaths and hospital admissions for respiratory diseases, for each muni ... | 2016 | 26247192 |
biochemical production of bioenergy from agricultural crops and residue in iran. | the present study assessed the potential for biochemical conversion of energy stored in agricultural waste and residue in iran. the current status of agricultural residue as a source of bioenergy globally and in iran was investigated. the total number of publications in this field from 2000 to 2014 was about 4294. iran ranked 21st with approximately 54 published studies. a total of 87 projects have been devised globally to produce second-generation biofuel through biochemical pathways. there are ... | 2016 | 27012716 |
heat causes oligomeric disassembly and increases the chaperone activity of small heat shock proteins from sugarcane. | small heat shock proteins (shsp) constitute an important chaperone family linked to conformational diseases. in plants, shsps prevent protein aggregation by acting as thermosensors and to enhance cell stress tolerance. sshsp17.2 and sshsp17.9 are the most highly expressed class i shsps in sugarcane. they exist as dodecamers at 20 degrees c and have distinct substrate specificities. therefore, they are useful models to study how class i shsps work. here we present data on the effects of heat on t ... | 2016 | 20137963 |
in situ enzyme aided adsorption of soluble xylan biopolymers onto cellulosic material. | the functional properties of cellulose fibers can be modified by adsorption of xylan biopolymers. the adsorption is improved when the degree of biopolymers substitution with arabinose and 4-o-methyl-glucuronic acid (meglca) side groups, is reduced. α-l-arabinofuranosidase (abfb) and α-d-glucuronidase (agua) enzymes were applied for side group removal, to increase adsorption of xylan from sugarcane (saccharum officinarum l) bagasse (bh), bamboo (bambusa balcooa) (bm), pinus patula (pp) and eucaly ... | 2016 | 27083357 |
predicting hotspots of human-elephant conflict to inform mitigation strategies in xishuangbanna, southwest china. | research on the spatial patterns of human-wildlife conflict is fundamental to understanding the mechanisms underlying it and to identifying opportunities for mitigation. in the state of xishuangbanna, containing china's largest tropical forest, an imbalance between nature conservation and economic development has led to increasing conflicts between humans and asian elephants (elephas maximus), as both elephant numbers and conversion of habitable land to rubber plantations have increased over the ... | 2016 | 27631976 |
continuous hydrogen production from agricultural wastewaters at thermophilic and hyperthermophilic temperatures. | the objective of this study was to investigate the effects of hydraulic retention time (hrt) (8 to 0.5 h) and temperature (55 to 75 °c) in two anaerobic fluidized bed reactors (afbr) using cheese whey (afbr-cw = 10,000 mg sugars l(-1)) and vinasse (afbr-v = 10,000 mg cod l(-1)) as substrates. decreasing the hrt to 0.5 h increased the hydrogen production rates in both reactors, with maximum values of 5.36 ± 0.81 l h2 h(-1) l(-1) in afbr-cw and 0.71 ± 0.16 l h2 h(-1) l(-1) in afbr-v. the optimal c ... | 2016 | 27987191 |
pilot scale biodiesel production from microbial oil of rhodosporidium toruloides debb 5533 using sugarcane juice: performance in diesel engine and preliminary economic study. | a successful pilot-scale process for biodiesel production from microbial oil (biooil) produced by rhodosporidium toruloides debb 5533 is presented. using fed-batch strategy (1000l working volume), a lipid productivity of 0.44g/l.h was obtained using a low-cost medium composed by sugarcane juice and urea. the microbial oil was used for biodiesel production and its performance was evaluated in diesel engine tests, showing very good performance, especially for the blend b20 sco, when operating at 2 ... | 2016 | 27969577 |
production of biodegradable plastic by polyhydroxybutyrate (phb) accumulating bacteria using low cost agricultural waste material. | polyhydroxybutyrates (phbs) are macromolecules synthesized by bacteria. they are inclusion bodies accumulated as reserve materials when the bacteria grow under different stress conditions. because of their fast degradability under natural environmental conditions, phbs are selected as alternatives for production of biodegradable plastics. the aim of this work was to isolate potential phb producing bacteria, evaluate phb production using agro-residues as carbon sources. | 2016 | 27955705 |
nutrient availability shapes the microbial community structure in sugarcane bagasse compost-derived consortia. | microbial communities (mcs) create complex metabolic networks in natural habitats and respond to environmental changes by shifts in the community structure. although members of mcs are often not amenable for cultivation in pure culture, it is possible to obtain a greater diversity of species in the laboratory setting when microorganisms are grown as mixed cultures. in order to mimic the environmental conditions, an appropriate growth medium must be applied. here, we examined the hypothesis that ... | 2016 | 27941835 |
biochemical compositions and fatty acid profiles in four species of microalgae cultivated on household sewage and agro-industrial residues. | the potential of four regional microalgae species was evaluated in relation to their cell growth and biomass production when cultured in the following alternative media: bio-composts of fruit/horticultural wastes (hb), sugarcane waste and vinasse (vb) chicken excrements (bce), raw chicken manure (rcm), and municipal domestic sewage (mds). the cultures were maintained under controlled conditions and their growth responses, productivities, biochemical compositions, and the ester profiles of their ... | 2016 | 27668876 |
effects of two different organic amendments addition to soil on sorption-desorption, leaching, bioavailability of penconazole and the growth of wheat (triticum aestivum l.). | this study investigated the effects of sugarcane bagasse compost (sbc) and chicken manure compost (cmc) on the sorption-desorption, leaching and bioavailability of the fungicide penconazole in soil in a laboratory setting. the autoclave-treated sbc or cmc was applied at 2.5% and 5.0% (w/w). results of batch equilibrium experiments exhibited that the sorption capacity of soils for penconazole was significantly promoted by the addition of sbc or cmc, whereas desorption of penconazole was drastical ... | 2016 | 26683765 |
morphological regional differences of epithelial cells along the midgut in diatraea saccharalis fabricius (lepidoptera: crambidae) larvae. | the sugarcane borer, diatraea saccharalis fabricius, is a pest to sugarcane and many other crops. this work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of d. saccharalis larvae. fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. there are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depend ... | 2016 | 18813743 |
host-plant associated genetic divergence of two diatraea spp. (lepidoptera: crambidae) stemborers on novel crop plants. | diatraea lineolata and diatraea saccharalis (lepidoptera: crambidae) are moths with stemboring larvae that feed and develop on economically important grasses. this study investigated whether these moths have diverged from a native host plant, corn, onto introduced crop plants including sorghum, sugarcane, and rice. diatraea larvae were collected from these four host plants throughout the year in el salvador and were reared on artificial diet until moths or parasitoids emerged. adult moths were s ... | 2016 | 28031813 |
sugarcane serine peptidase inhibitors, serine peptidases, and clp protease system subunits associated with sugarcane borer (diatraea saccharalis) herbivory and wounding. | sugarcane's (saccharum spp.) response to diatraea saccharalis (f.) (lepidoptera: (crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane expressed sequence tags (ests) encoding serine peptidase inhibitors, serine peptidases. and clp protease system subunits. our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. among the upregulated genes, nine were similar to serine peptidase inhibitors and four were ... | 2016 | 27598134 |
restriction site associated dna (rad) for de novo sequencing and marker discovery in sugarcane borer, diatraea saccharalis fab. (lepidoptera: crambidae). | we present the development of a genomic library using radseq (restriction site associated dna sequencing) protocol for marker discovery that can be applied on evolutionary studies of the sugarcane borer diatraea saccharalis, an important south american insect pest. a radtag protocol combined with illumina paired-end sequencing allowed de novo discovery of 12 811 snps and a high-quality assembly of 122.8m paired-end reads from six individuals, representing 40 gb of sequencing data. approximately ... | 2016 | 27571734 |
transgenic sugarcane with a cry1ac gene exhibited better phenotypic traits and enhanced resistance against sugarcane borer. | we developed sugarcane plants with improved resistance to the sugarcane borer, diatraea saccharalis (f). an expression vector pgcry1ac0229, harboring the cry1ac gene and the selectable marker gene, bar, was constructed. this construct was introduced into the sugarcane cultivar fn15 by particle bombardment. transformed plantlets were identified after selection with phosphinothricin (ppt) and basta. plantlets were then screened by pcr based on the presence of cry1ac and 14 cry1ac positive plantlet ... | 2016 | 27093437 |
badnaviruses: the current global scenario. | badnaviruses (family: caulimoviridae; genus: badnavirus) are non-enveloped bacilliform dna viruses with a monopartite genome containing about 7.2 to 9.2 kb of dsdna with three to seven open reading frames. they are transmitted by mealybugs and a few species by aphids in a semi-persistent manner. they are one of the most important plant virus groups and have emerged as serious pathogens affecting the cultivation of several horticultural crops in the tropics, especially banana, black pepper, cocoa ... | 2016 | 27338451 |
microsatellite loci and genetic structure of artificial populations of cotesia flavipes (hymenoptera, braconidae). | cotesia flavipes (cameron) is a parasitoid wasp used in the biological control of the sugarcane borer (diatraea saccharalis) (fabr., 1794). studies on the genetic diversity of c. flavipes are hampered by the lack of highly polymorphic molecular markers. in this report, a set of 11 microsatellite loci were developed from an enriched library of c. flavipes. four microsatellite loci were polymorphic and were screened in 212 c. flavipes individuals (183 females and 29 males) that were randomly sampl ... | 2016 | 27886334 |
quality of the exotic parasitoid cotesia flavipes (hymenoptera: braconidae) does not show deleterious effects after inbreeding for 10 generations. | although the parasitoid cotesia flavipes (cameron) has proven effective in controlling sugarcane borer diatraea saccharalis (fabricius) for many years, concern has arisen over the quality of individuals produced at large scales. the parasitoid has been reared in laboratories in brazil for more than 40 years, with no new introductions of new populations during that period. since the quality of the parasitoids was not verified at the time of the species' introduction in brazil, we do not know if t ... | 2016 | 27509087 |
a two-agent model applied to the biological control of the sugarcane borer (diatraea saccharalis) by the egg parasitoid trichogramma galloi and the larvae parasitoid cotesia flavipes. | the paper is aimed at a methodological development in biological pest control. the considered one pest two-agent system is modelled as a verticum-type system. originally, linear verticum-type systems were introduced by one of the authors for modelling certain industrial systems. these systems are hierarchically composed of linear subsystems such that a part of the state variables of each subsystem affect the dynamics of the next subsystem. recently, verticum-type system models have been applied ... | 2016 | 26911807 |
tithonia diversifolia as a supplementary feed for dairy cows. | the objective of this study was to examine the effects of tithonia diversifolia as a supplementary forage on dairy cow performance and methane production. nine lactating holstein × zebu dairy cows (519 ± 53.3 kg of body weight and 66 ± 13.3 d in milk) were paired by milk yield (21.3 ± 2.34 kg/d) and body weight and randomly assigned to three dietary treatments in a latin square design with 21-d experimental periods (14 d for diet adaptation and 7 d for measurements and sample collection). the di ... | 2016 | 27906983 |
the comparison of digestibility of treated sugarcane tops silage by bacteria or whole microorganisms of holstein cow and buffalo rumen. | the aim of this study was to evaluate the effects of adding sulfuric acid to sugarcane tops silage on rumen bacteria and whole rumen microorganisms (wrm) and compare the digestibility of sugarcane tops treated with different amount of urea, molasses and sulfuric acid between holstein cow and khouzestan buffalo. regardless of the type of the treatment, potential of gas production (b) by cow wrm (130.670 ml) was more than buffalo (104.060 ml) (p < 0.05), but the rate of gas production (c) by buffa ... | 2016 | 27872716 |
lessons learned from the microbial ecology resulting from different inoculation strategies for biogas production from waste products of the bioethanol/sugar industry. | during strategic planning of a biogas plant, the local availability of resources for start-up and operation should be taken into consideration for a cost-efficient process. because most bioethanol/sugar industries in brazil are located in remote areas, the use of fresh cattle manure from local farms could be a solution for the inoculation of the biogas process. this study investigated the diversity and dynamics of bacterial and archaeal communities and the performance of biogas reactors inoculat ... | 2016 | 27429647 |
assessment of feed resources, feeding practices and coping strategies to feed scarcity by smallholder urban dairy producers in jimma town, ethiopia. | smallholder dairy production is increasingly becoming popular in jimma town. however, feed shortage is a major constraint to dairy production. the objectives of this study was to assess feed resources, feeding practices and farmers' perceived causes of feed shortage and coping strategies to feed scarcity in smallholder dairy producers in jimma town, oromia regional state, ethiopia. a total of 54 randomly selected dairy farmers were interviewed using a pre-tested structured questionnaire and thro ... | 2016 | 27375986 |
applying functional metagenomics to search for novel lignocellulosic enzymes in a microbial consortium derived from a thermophilic composting phase of sugarcane bagasse and cow manure. | environments where lignocellulosic biomass is naturally decomposed are sources for discovery of new hydrolytic enzymes that can reduce the high cost of enzymatic cocktails for second-generation ethanol production. metagenomic analysis was applied to discover genes coding carbohydrate-depleting enzymes from a microbial laboratory subculture using a mix of sugarcane bagasse and cow manure in the thermophilic composting phase. from a fosmid library, 182 clones had the ability to hydrolyse carbohydr ... | 2016 | 27350392 |
effect of treating sugarcane bagasse with urea and calcium hydroxide on feed intake, digestibility, and rumen fermentation in beef cattle. | four beef cattle with initial body weight of 283 ± 14 kg were randomly allocated according to a 4 × 4 latin square design to study on the effect of feeding sugarcane bagasse (sb) treated with urea and/or calcium hydroxide (ca(oh)2) on feed intake, digestibility, and rumen fermentation. the treatments were as follows: rice straw (rs), untreated sb (sb), 4 % urea-treated sb (sbu), and 2 % urea + 2 % ca(oh)2-treated sb (sbuc), respectively. the results revealed that cattle fed with sbu and sbuc had ... | 2016 | 27139254 |
flint corn grain processing and citrus pulp level in finishing diets for feedlot cattle. | four trials were conducted to evaluate the effects of flint corn processing and the replacement of corn with citrus pulp (cip) in diets for nellore feedlot cattle. in a 103-d finishing trial, 216 nellore bulls (350 ± 24 kg initial bw) were used in a randomized complete block design with a 2 × 4 factorial arrangement of treatments. factors included 2 processing methods, either ground corn (gc) or steam-flaked corn (fc), with cip replacing each corn type at 4 levels (0, 25, 50, and 75% of dm). all ... | 2016 | 27065137 |
performance and metabolism of calves fed starter feed containing sugarcane molasses or glucose syrup as a replacement for corn. | the aim of this study was to evaluate the effect of replacing corn grain for sugar cane molasses (mo) or glucose syrup (gs) in the starter concentrate on performance and metabolism of dairy calves. thirty-six individually housed holstein male calves were blocked according to weight and date of birth and assigned to one of the starter feed treatments, during an 8 week study: i) starter containing 65% corn with no mo or gs (0mo); ii) starter containing 60% corn and 5% mo (5mo); iii) starter contai ... | 2016 | 26954149 |
nutritional and productive performance of dairy cows fed corn silage or sugarcane silage with or without additives. | the objective of this study was to compare the intake, digestibility, and performance of dairy cows fed corn silage, fresh sugarcane, and sugarcane ensiled in three different forms. twenty-five holstein cows at 114 ± 12.6 days in milk (dim) were used. a randomized block design was adopted, using an arrangement of repeated measures over time. the following treatments were tested: corn silage (cs); fresh sugarcane (fs); sugarcane silage without additives (scs); sugarcane silage enriched with calci ... | 2016 | 26898688 |
effects of various roughage levels with whole flint corn grain on performance of finishing cattle. | performance responses to steam flaking flint corn as well as to the addition of roughage to finishing diets composed of whole flint corn were evaluated. ninety-six nellore bulls were stratified by initial bw (373 ± 11 kg) and randomly allotted to 16 feedlot pens (6 bulls/pen) in a randomized complete block design with 4 replicates/treatment. dietary treatments for the 86-d feeding trial consisted of (dm basis) 1) 78.8% steam-flaked flint corn with 6% sugarcane bagasse and 0.20% urea, 2) 85% whol ... | 2016 | 26812339 |
comparison of start-up strategies and process performance during semi-continuous anaerobic digestion of sugarcane filter cake co-digested with bagasse. | the anaerobic digestion of sugarcane filter cake and the option of co-digestion with bagasse were investigated in a semi-continuous feeding regime to assess the main parameters used for large-scale process designing. moreover, fresh cattle manure was considered as alternative inoculum for the start-up of biogas reactors in cases where digestate from a biogas plant would not be available in remote rural areas. experiments were carried out in 6 lab-scale semi-continuous stirred-tank reactors at me ... | 2016 | 26584558 |
comparison between brazilian agro-wastes and activated carbon as adsorbents to remove ni(ii) from aqueous solutions. | this research was performed to find an alternative, low-cost, competitive, locally available and efficient adsorbent to treat nickel (ni) containing effluents. for this purpose, several brazilian agro-wastes like sugarcane bagasse (scb), passion fruit wastes (pfw), orange peel (op) and pineapple peel (pp) were compared with an activated carbon (ac). the adsorbents were characterized. effects of fundamental factors affecting the adsorption were investigated using batch tests. kinetic and equilibr ... | 2016 | 27232408 |
the core root microbiome of sugarcanes cultivated under varying nitrogen fertilizer application. | diazotrophic bacteria potentially supply substantial amounts of biologically fixed nitrogen to crops, but their occurrence may be suppressed by high nitrogen fertilizer application. here, we explored the impact of high nitrogen fertilizer rates on the presence of diazotrophs in field-grown sugarcane with industry-standard or reduced nitrogen fertilizer application. despite large differences in soil microbial communities between test sites, a core sugarcane root microbiome was identified. the sug ... | 2016 | 26032777 |
expression of arabidopsis bax inhibitor-1 in transgenic sugarcane confers drought tolerance. | the sustainability of global crop production is critically dependent on improving tolerance of crop plants to various types of environmental stress. thus, identification of genes that confer stress tolerance in crops has become a top priority especially in view of expected changes in global climatic patterns. drought stress is one of the abiotic stresses that can result in dramatic loss of crop productivity. in this work, we show that transgenic expression of a highly conserved cell death suppre ... | 2016 | 26872943 |
recent trends in bioethanol production from food processing byproducts. | the widespread use of corn starch and sugarcane as sources of sugar for the production of ethanol via fermentation may negatively impact the use of farmland for production of food. thus, alternative sources of fermentable sugars, particularly from lignocellulosic sources, have been extensively investigated. another source of fermentable sugars with substantial potential for ethanol production is the waste from the food growing and processing industry. reviewed here is the use of waste from potat ... | 2016 | 27565674 |
utilization of vinasses as soil amendment: consequences and perspectives. | vinasses are a residual liquid generated after the production of beverages, such as mezcal and tequila, from agave (agave l.), sugarcane (saccharum officinarum l.) or sugar beet (beta vulgaris l.). these effluents have specific characteristics such as an acidic ph (from 3.9 to 5.1), a high chemical oxygen demand (50,000-95,000 mg l(-1)) and biological oxygen demand content (18,900-78,300 mg l(-1)), a high total solids content (79,000 and 37,500 mg l(-1)), high total volatile solids 79,000 and 82 ... | 2016 | 27441131 |
genome editing in sugarcane: challenges ahead. | genome editing opens new and unique opportunities for researchers to enhance crop production. until 2013, the zinc finger nucleases (zfns) and transcription activator-like effector nucleases (talens) were the key tools used for genome editing applications. the advent of rna-guided engineered nucleases - the type ii clustered regularly interspaced short palindromic repeat (crispr)/cas9 (crispr-associated) system from streptococcus pyogenes holds great potential since it is simple, effective and m ... | 2016 | 27790238 |
characterization of a sorghum mosaic virus (srmv) isolate in china. | sorghum mosaic virus (srmv), a causal agent of the destructive sugarcane mosaic disease, has a global presence. an isolate of srmv infecting a commercially-grown sugarcane plant was recovered from the hainan province of china. the virions were visualized by an electron microscope, and the coat proteins (cps) were sequenced by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry. discrepancies between the cp predicted and actual amino acid sequ ... | 2016 | 26981005 |
genomic variability and molecular evolution of asian isolates of sugarcane streak mosaic virus. | sugarcane streak mosaic virus (scsmv), an economically important causal agent of mosaic disease of sugarcane, is a member of the newly created genus poacevirus in the family potyviridae. in this study, we report the molecular characterization of three new scsmv isolates from china (yn-yz211 and hn-yz49) and myanmar (mya-formosa) and their genetic variation and phylogenetic relationship to scsmv isolates from asia and the type members of the family potyviridae. the complete genome of each of the ... | 2016 | 26973230 |
genetic structure of populations of sugarcane streak mosaic virus in china: comparison with the populations in india. | sugarcane streak mosaic virus (scsmv) causes mosaic and streak symptoms on sugarcane and sorghum crops, and has a broad host range. scsmv is a member of the genus poacevirus in the family potyviridae.ten scsmv isolates were collected from sugarcane plants showing mosaic and streaking in southern china from 2009-2011. sequence-based phylogenetic and population genetic analyses were conducted using four partial genomic sequences covering the full genomes. these analyses were used to estimate the s ... | 2016 | 26432446 |
molecular variability and distribution of sugarcane mosaic virus in shanxi, china. | sugarcane mosaic virus (scmv) is responsible for large-scale economic losses in the global production of sugarcane, maize, sorghum, and some other graminaceous species. to understand the evolutionary mechanism of scmv populations, this virus was studied in shanxi, china. a total of 86 maize leaf samples (41 samples in 2012 and 45 samples in 2013) were collected from 4 regions of shanxi. | 2016 | 26987060 |
the complete genome sequences of two naturally occurring recombinant isolates of sugarcane mosaic virus from iran. | sugarcane mosaic virus (scmv) is the most prevalent virus causing sugarcane mosaic and maize dwarf mosaic diseases. here, we presented the first two complete genomic sequences of iranian scmv isolates, nra and zra from sugarcane and maize. the complete genome sequences of nra and zra were, respectively, 9571 and 9572 nucleotides (nt) in length, excluding the 3'-terminal poly(a) tail. both isolates contained a 5'-untranslated region (utr) of 149 nt, an open reading frame of 9192 nt encoding a pol ... | 2016 | 26905544 |
enhanced cellulase production by trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates. | the use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. this work evaluates the production of cellulase enzymes using glycerol for high cell density growth of trichoderma harzianum followed by induction with a cellulosic material. firstly, the influence of the carbon source used in the pre-culture step was investigated in terms o ... | 2016 | 26883662 |
purification and characterization of endo β-1,4-d-glucanase from trichoderma harzianum strain hzn11 and its application in production of bioethanol from sweet sorghum bagasse. | an acidophilic-solvent-thermostable endo β-1,4-d-glucanase produced from a potential trichoderma harzianum strain hzn11 was purified to homogeneity by deae-sepharose and sephadex g-100 chromatography with 33.12 fold purification with specific activity of 66.25 u/mg and molecular mass of ~55 kda. the optimum temperature and ph were 60 °c and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. it showed stability between ph 4.5-6.0 and temperature between 50-70 °c indicating thermostabi ... | 2016 | 28330171 |
detection of puccinia kuehnii causing sugarcane orange rust with a loop-mediated isothermal amplification-based assay. | puccinia kuehnii is a fungal pathogen that causes orange rust in sugarcane, which is now prevalent in many countries. at the early stage of disease, it is almost indistinguishable from brown rust, which is caused by puccinia melanocephala. although several pcr assays are available to detect these diseases, the loop-mediated isothermal amplification (lamp)-based assay has been reported to be more economical and easier to perform. under isothermal conditions, dna is amplified with high specificity ... | 2016 | 26837389 |
surface polysaccharides and quorum sensing are involved in the attachment and survival of xanthomonas albilineans on sugarcane leaves. | xanthomonas albilineans, the causal agent of sugarcane leaf scald, is a bacterial plant pathogen that is mainly spread by infected cuttings and contaminated harvesting tools. however, some strains of this pathogen are known to be spread by aerial means and are able to colonize the phyllosphere of sugarcane before entering the host plant and causing disease. the objective of this study was to identify the molecular factors involved in the survival or growth of x. albilineans on sugarcane leaves. ... | 2016 | 25962850 |
the mating-type locus b of the sugarcane smut sporisorium scitamineum is essential for mating, filamentous growth and pathogenicity. | sporisorium scitamineum is the causal agent of sugarcane smut, which is one of the most serious constraints to global sugarcane production. s. scitamineum and ustilago maydis are two closely related smut fungi, that are predicted to harbor similar sexual mating processes/system. to elucidate the molecular basis of sexual mating in s. scitamineum, we identified and deleted the ortholog of mating-specific u. maydis locus b, in s. scitamineum. the resultant b-deletion mutant was defective in mating ... | 2016 | 26563415 |
identification of putative candidate genes for red rot resistance in sugarcane (saccharum species hybrid) using ld-based association mapping. | red rot is a serious disease of sugarcane caused by the fungus colletotrichum falcatum that has a colossal damage potential. the fungus, prevalent mainly in the indian sub-continent, keeps on producing new pathogenic strains leading to breakdown of resistance in newly released varieties and hence the deployment of linked markers for marker-assisted selection for resistance to this disease can fine tune the breeding programme. this study based on a panel of 119 sugarcane genotypes fingerprinted f ... | 2016 | 26961118 |
draft genome sequence of colletotrichum falcatum - a prelude on screening of red rot pathogen in sugarcane. | colletotrichum falcatum, a concealed fungal ascomycete causes red rot, which is a serious disease in sugarcane. it infects economically important stalk tissues, considered as store house of sugar in sugarcane. the study is to find genetic complexities of c. falcatum in establishing this as a stalk infecting pathogen and to decipher the unique lifestyle of this pathogen using ngs technology. we report the draft genome of c. falcatum of about 48.16 mb in size with 12,270 genes. the genome sequence ... | 2016 | 26958090 |
biotrophic interaction of sporisorium scitamineum on a new host--saccharum spontaneum. | sporisorium scitamineum is a biotrophic smut fungus harbored inside the smut gall on the top internodal region of saccharum spontaneum, a wild relative of sugarcane (saccharum officinarum). the interactions of spined conidia of s. scitamineum with s. spontaneum were examined during the different stages of plant growth starting from the bud stage to the decaying stage. the spores in the soil from the polyetic inocula grew into confined epidermal cells of the buds and finally sporulated in the top ... | 2016 | 26642345 |
d-003 (saccharum officinarum): the forgotten lipid-lowering agent. | reduction of elevated cholesterol levels, particularly low-density lipoprotein cholesterol (ldl-c), is essential in primary and secondary prevention of cardiovascular disease (cvd). therefore there is still a large need for new effective drugs, which would be able to essentially reduce ldl-c and in the consequence cv residual risk. d-003 is a mixture of high aliphatic primary acids purified from sugarcane (saccharum officinarum) wax. it showed promising hypocholesterolemic effects in both animal ... | 2016 | 27751878 |
composition, taste, aroma, and antioxidant activity of solidified noncentrifugal brown sugars prepared from whole stalk and separated pith of sugarcane (saccharum officinarum l.). | in this study, 2 types of solidified noncentrifugal brown sugars (w-ncs and p-ncs) were prepared from the whole stalk and separated pith, respectively, of raw sugarcane (saccharum officinarum l.). these products were discriminated in terms of their quality attributes, including color, sugars and minerals composition, taste, aroma, and antioxidant activity. the brown color of p-ncs was clearly different compared with that of w-ncs with a color difference value (δe* ) of 9.36. there was no differe ... | 2016 | 27780296 |