Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| experimental leprosy in the armadillo and nude mice: comparative histobacteriology and ultrastructure. | in last 14 years, armadillo has proved an ideal animal model for studying experimental leprosy and mass production of mycobacterium leprae. however recently a number of groups working with nude mice have claimed its ability as a better experimental model as far as leprosy research and production of leprosy bacilli is concerned. we therefore decided to compare experimental m. leprae infection of both armadillo and nude mice. we compared the degree of infection as well as the physiological and mor ... | 1984 | 6398592 |
| occurrence of antigen bcg 60 in leprosy derived corynebacteria and other coryneforms. | previous studies have shown that anti bcg 60 monoclonal antibody could recognize major antigen a 7 of m. leprae. in the present investigation we attempted to search the presence of the same antigen from the strains of leprosy derived corynebacteria (ldc+), which were isolated from leprosy lesions and were supposed to be the cultivable form of the leprosy bacillus. a comparative study was equally performed on 7 strains of corynebacteria which were isolated in france and had no relation with lepro ... | 1984 | 6398594 |
| concentration-dependent effects of mycobacteria on the stimulation of murine t-cell clones. | m. leprae produced concentration-dependent bimodal effects in cultures of m. leprae-immune lymphocytes. at low to intermediate concentrations, m. leprae and other species of mycobacteria stimulated lymphoproliferation of m. leprae t-helper cell clones, whereas at high concentrations responses were reduced. lymphokine production by m. leprae-immune t-cell hybridomas also showed bimodal responses to different concentrations of m. leprae. these results indicate that mycobacterial antigen may direct ... | 1984 | 6398595 |
| antibodies to mycobacterium leprae detected by enzyme linked (elisa) and radio (ria) immunoassay: comparison among families with leprosy patients. | 1984 | 6398597 | |
| igm antibodies against phenolic glycolipid i from mycobacterium leprae in leprosy sera: relationship to bacterial index and erythema nodosum leprosum. | serum igm antibodies against mycobacterium leprae-derived phenolic glycolipid i (pg) were determined in 121 leprosy patients, in contacts and controls by an enzyme-linked immunosorbent assay technique. anti-pg igm levels correlated with disease classification, increasing from the tuberculoid towards the lepromatous pole of the disease spectrum. there was a linear correlation between serum igm pg-antibody levels and bacillary index (bi), a measure of bacterial load. elevated anti-pg igm in bacill ... | 1984 | 6398598 |
| proceedings of the who--esm--sfm joint meeting on mycobacterium leprae. institut pasteur, june 12-14, 1984. | 1984 | 6398602 | |
| host-grown mycobacterium leprae: a credible microorganism. | in this paper recent advances in knowledge of the following aspects of mycobacterium leprae will be reviewed: animal sources, ultrastructure, lipid composition, wall structure, molecular biology, metabolic capabilities and measurement of viability. it will be proposed that isolated from infected tissues are consistent, that is they share chemical and biochemical features although originating from many parts of the world, are unique, that is they have properties possessed by no other microorganis ... | 1984 | 6398603 |
| "drug-resistant proportion test" for m. leprae to quantify the proportion of drug-resistant m. leprae in a sample using the mouse foot pad. | the mouse foot pad test has not previously been used quantitatively to discriminate between samples of mycobacterium leprae with differing proportions of drug-resistant m. leprae. the "drug-resistant proportion test" is a simple modification of the routine mouse foot pad test. it is demonstrated to distinguish between samples of m. leprae with a tenfold difference in the proportion of dapsone-resistant m. leprae. | 1984 | 6399066 |
| primary dapsone-resistant leprosy in san francisco. | the dapsone sensitivity of strains of mycobacterium leprae from 54 multibacilliferous untreated leprosy patients presenting to the united states public health service hospital in san francisco, california, u.s.a., from 1978 to 1981 was studied by mouse foot pad inoculation. m. leprae from 53 patients were found fully sensitive to dapsone. m. leprae from one patient were resistant to only the lowest dietary level of dapsone, 0.0001%, since growth of the bacilli was inhibited by higher and clinica ... | 1984 | 6399067 |
| abortive infection of mycobacterium leprae by the mycobacteriophage d29. | the interactions of mycobacteriophage d29 and mycobacterium leprae were examined. it was demonstrated that after adsorption d29 injected its dna in m. leprae. while the synthesis of host proteins and lipids were inhibited in m. tuberculosis and in m. smegmatis during infection by d29, the results were inconclusive in the case of m. leprae because these bacteria did not incorporate the appropriate substrates. | 1984 | 6399068 |
| wall biosynthesis: a possible site of action for new antimycobacterial drugs. | 1984 | 6399069 | |
| six cases of dapsone-resistant tuberculoid leprosy. | 1984 | 6399070 | |
| activity of cycloserine and structurally related compounds against m. leprae-infected mice. | 1984 | 6399071 | |
| mycobacterium x identified as mycobacterium avium intracellulare (probably mixed with m. leprae in early subcultures) | 1984 | 6399072 | |
| in vitro cultivation of mycobacterium x from mycobacterium leprae infected tissues in propane-tetradecane medium (a preliminary communication). | host grown mycobacterium leprae and cultures of mycobacterium x, cultivated from m. leprae infected armadillo and human specimens, were inoculated into propane and propane-tetradecane media. the media contained in one litre distilled water kh2po4, 7 g; na2hpo4, 0.5 g; (nh4)2so4, 2 g; mgso4, 0.1 g; ferric ammonium citrate, 20 mg and yeast extract (difco), 0.1 g. twenty ml media, distributed into each of 50 ml screw cap tubes, were inoculated with the bacilli and bubbled aseptically for 10 s with ... | 1984 | 6399185 |
| [observation of antileprotic drug resistance]. | 1984 | 6399285 | |
| mode of action of sulphones. | 1984 | 6399286 | |
| ultrastructural characterization of normal and damaged membranes of mycobacterium leprae and of cultivable mycobacteria. | microdensitometry showed that the membrane profiles of normal cultivable mycobacteria were very asymmetric (outer layer denser and thicker than the inner layer), while the profiles of normal-looking m. leprae in lepromatous patients, in experimentally infected armadillos and in nude mice were approximately symmetric; moreover, the membrane of m. leprae was thicker than that of cultivable species. using two cytochemical methods for the ultrastructural detection of periodic acid-schiff (pas)-posit ... | 1984 | 6427399 |
| occasional review--hla and leprosy: a re-evaluation. | 1984 | 6431213 | |
| experimental mycobacterial infections of cba/n mice. | in an effort to assess the contribution of b-lymphocyte-mediated mechanisms to the immune responses to several mycobacteria, responses to these intracellular pathogens were compared in immunologically normal cba/cahn mice and in histocompatible cba/n (xid) mice, which exhibit abnormalities of b-lymphocyte function. swelling in response to local inoculation with mycobacterium marinum was significantly greater in the hind feet of cba/cahn mice than in those of cba/n mice, but the difference was ve ... | 1984 | 6381393 |
| [lipid composition of cultivable mycobacteria isolated from livers of armadillos infected by mycobacterium leprae]. | four bacterial strains isolated from two livers of armadillos experimentally infected with mycobacterium leprae were studied. lipids obtained after saponification and methylation and complex lipids obtained by solvent extraction were examined. the presence of mycolates showed that the four strains belonged to the genus mycobacterium, but the mycolate patterns, identical for the four strains, were different from those of all strains studied so far. three of these strains contained phthioceranic a ... | 1984 | 6380370 |
| fluorescence of mycobacterium leprae. | 1984 | 6380513 | |
| in vitro stimulation of lymphocytes with an antigen fraction prepared from mycobacterium leprae and tuberculin ppd in contacts and non-contacts of leprosy patients. | an antigen fraction from mycobacterium leprae, called mlw1, was used as stimulator in the lymphocyte stimulation test, for comparison with tuberculin ppd. the test was performed in three groups of contacts of leprosy patients with various degree of exposure: (1) close contacts, (2) healthy occupational contacts and (3) non-close contacts and, in addition, in a group of bcg vaccinated and non-exposed controls. the mlw1 preparation induced moderate to strong responses in all three groups of contac ... | 1984 | 6380833 |
| isolation and analysis of circulating immune complexes in leprosy. | circulating immune complexes (cic) were isolated by two antigen nonspecific methods from 60 leprosy patients belonging to borderline tuberculoid (bt) and lepromatous (ll) types with and without reactions. cic were elevated in both bt and ll reactions. cic from bt in reaction (btr) were found to consist largely of igg and c3, whereas, c-reactive protein could be found in cic from ll reactions (lr). in addition, igm and rheumatoid factor were demonstrated in the complexes of lr patients who had ma ... | 1984 | 6380842 |
| immunochemical characterization of mycobacterium leprae antigens by the sds-polyacrylamide gel electrophoresis immunoperoxidase technique (sgip) using patients' sera. | in this study the sds-polyacrylamide gel electrophoresis immunoperoxidase (sgip) assay was used for characterizing the antigenic components of mycobacterium leprae using patients' sera. this technique involved the separation of mycobacterial sonicates on sds-polyacrylamide gels, longitudinal sectioning of the gels, incubation with patients' sera and visualization of the antigen-antibody complexes by the indirect immunoperoxidase technique. a number of antigens present in m. leprae sonicates were ... | 1984 | 6378452 |
| c-reactive protein and apob containing lipoproteins are associated with mycobacterium leprae in lesions of human leprosy. | skin biopsies from patients with leprosy across the spectrum from tuberculoid (tt) to lepromatous (ll), including histoid lepromas and erythema nodosum leprosum (enl) reactions, were stained immunohistochemically for the presence of c-reactive protein (crp) and the apolipoprotein, apob. mycobacterium leprae bacillary material comprising cell walls, cytoplasmic and soluble components was present with increasing abundance towards the lepromatous end of the spectrum and always stained positively wi ... | 1984 | 6378453 |
| the effect of cortisone on mycobacterium leprae-infected mice. | 1984 | 6379352 | |
| dapsone-resistant leprosy. | 1984 | 6379353 | |
| [effect of ultrasonic waves on the growth intensity of mycobacterium leprae in liquid nutrient media]. | ultrasound-treated m. leprae grow in liquid culture media at a greater rate than untreated ones. ultrasound-treated m. leprae retain their capacity for intensive growth after their passage in mice. | 1984 | 6375218 |
| humans respond predominantly with igm immunoglobulin to the species-specific glycolipid of mycobacterium leprae. | the immunoglobulin classes of the antibody response to the species-specific phenolic glycolipid antigen of mycobacterium leprae have been characterized for serum specimens from 78 patients with leprosy. these patients included the entire clinical spectrum from paucibacillary to multibacillary disease, including polar tuberculoid (tt; 11 patients), borderline tuberculoid (bt; 15), borderline (bb; 17), borderline lepromatous (bl; 13), and lepromatous (ll; 22)--clinical classifications according to ... | 1984 | 6376654 |
| antigen specific macrophage-lymphocyte interaction in lepromatous leprosy. | peripheral blood derived macrophages from lepromatous leprosy patients were unable to interact with lymphocytes in the presence of m. leprae. this lack of interaction is probably not associated with membrane hla-dr antigens since trypsin and colchicine restored m. leprae induced depression in the latter but were unable to bring about a positive interaction. two possible defects exist therefore in the lepromatous macrophage. these are an innate inability to process and present m. leprae antigens ... | 1984 | 6376810 |
| [advances in the chemotherapy of leprosy]. | the chemotherapy of multibacillary leprosy with dapsone alone has resulted after 15-20 years in the selection and the diffusion of dapsone resistant mycobacterium leprae. to overcome dapsone resistance, to prevent the selection of organisms resistant to other drugs and to kill the largest proportion of sensitive m. leprae, the chemotherapy of leprosy must rely, as chemotherapy of tuberculosis, upon the combination of several drugs. rifampicin is included in all drug combinations recommended by w ... | 1984 | 6377002 |
| anti-mycobacterium leprae antibodies in urine from lepromatous patients examined by crossed immunoelectrophoresis and radioimmunoassay. | precipitating anti-mycobacterium leprae antibodies were found in concentrated urine samples from 21 out of 42 lepromatous patients. these antibodies were directed against m. leprae antigens 5, 6, and 7. in a radioimmunoassay for anti-m. leprae antibodies, 90% of these patients had higher antibody levels in their urine than control persons. there was a positive correlation between anti-m. leprae antibody levels in serum and urine. the advantages of using atraumatically collected samples like urin ... | 1984 | 6377478 |
| antibodies to mycobacterial arabinomannan in leprosy: correlation with reactional states and variation during treatment. | an enzyme-linked immunosorbent assay was used to measure antibody to mycobacterial arabinomannan in serial serum specimens obtained over the initial 12-31 months of therapy from nine patients with leprosy. the antibody level in pretreatment sera was directly proportional to the quantity of mycobacterium leprae present in each patient as assessed by six-site scrapings (r = 0.75). the three patients with the lowest antibody levels (od 0.1-0.3) had uncomplicated courses and their levels declined sl ... | 1984 | 6373624 |
| adenosine triphosphate content of mycobacterium leprae from leprosy patients. | mycobacterium leprae obtained from randomly selected lepromatous leprosy patients were used to evaluate the atp assay technique for detecting viability of these cells. the findings were further confirmed by the standard mouse foot pad technique. while the latter takes about 8-12 months to obtain any valid information on the status of m. leprae, the atp data can be generated within hours and at much lower cost. it is hoped that the atp data could also instantaneously identify viable bacilli from ... | 1984 | 6373626 |
| attempts at cultivation of mycobacterium leprae in macrophages from susceptible animal hosts. | macrophages from nude mice, nude rats, and armadillos were cultured in vitro and examined for their ability to support mycobacterium leprae. no significant growth of this organism were observed after over 200 days of culture. no significant benefit was derived from modifying culture conditions or from variations in the source of macrophages or the source of m. leprae. | 1984 | 6373627 |
| ultrastructural features of macrophages of armadillos infected with actively multiplying mycobacterium leprae. | experimental leprosy lesions in the armadillo (dasypus novemcinctus) were studied by freeze etching and ultrathin sectioning. infected macrophages have distinct intracytoplasmic foamy structures in the form of spherical droplets accumulated around multiplying bacilli. this finding is the same as those observed in human lepra cells and nude mice macrophages infected with m. leprae. | 1984 | 6373628 |
| electron microscopic study of leprosy in a mangabey monkey (natural infection). | ultrastructural features of the leproma of a) a naturally infected mangabey monkey, and lepromas and liver of b) a passage mangabey monkey, c) a rhesus monkey, d) an african green monkey, and e) a nine-banded armadillo inoculated with leprosy bacilli isolated from the leproma of a naturally infected mangabey monkey were studied by the freeze-etching technique. the size, shape, and ultrastructural features of leprosy bacilli in the phagolysosomes of macrophages in all of these samples were essent ... | 1984 | 6373629 |
| multiple axonal myelination in the experimental mouse leprosy model. | 1984 | 6373630 | |
| oxidation of carbon sources through the tricarboxylic acid cycle in mycobacterium leprae grown in armadillo liver. | all the enzymes of the tricarboxylic acid cycle have now been demonstrated in extracts of mycobacterium leprae grown in armadillo liver. many were also present in homogenates of host-tissue, but biochemical evidence is presented which indicates that all enzymes detected in extracts from m. leprae were authentic bacterial enzymes. further evidence for a complete tricarboxylic acid cycle in m. leprae was obtained by first establishing that citrate could be taken up and catabolized by whole m. lepr ... | 1984 | 6374022 |
| in vitro studies on the effect of levamisole in lepromatous leprosy. | our previous studies have demonstrated a defective macrophage response to m. leprae in lepromatous leprosy patients. in the present study we report the restoration of fc receptor and hla-dr antigen expression as well as antigen specific macrophage-lymphocyte interaction on treatment with levamisole in vitro. these results indicate that levamisole activates the macrophages which in turn results in an improved cell mediated immune response in lepromatous leprosy. this may also be applicable in oth ... | 1984 | 6374154 |
| dopa-oxidase activity on icrc bacilli. | presence of o-phenoloxidase is regarded as m. leprae specific character. this enzyme activity was found to be present in icrc bacilli, strain c-44. though this strain is cultivable 'in vitro', the expression of dopa-oxidase activity strongly suggests that it carries m. leprae genome. the icrc bacilli, therefore, may thus from a group of m. leprae culture isolates, distinct from other known cultivable mycobacteria which do not possess this enzyme activity. | 1984 | 6434641 |
| [sensitivity and specificity of fluorescent leprosy antibody absorption (fla-abs) test for detecting subclinical infection by mycobacterium leprae]. | 1984 | 6437637 | |
| antibacterial monoclonal antibodies and the dawn of a new era in the control of infection. | literature reports concerned with monoclonal antibodies against bacteria, or their toxins, which are pathogens for man and animals were surveyed. these antibodies have important potential uses in human and veterinary pathology and medicine. they are likely to become key elements in a fast progression toward a more complete understanding and control of infectious diseases and of toxin poisoning. a new area of bacteriology relevant to sanitary engineering is also being advanced with the help of an ... | 1984 | 6438759 |
| production and characterization of monoclonal antibodies to mycobacterium tuberculosis, m. bovis (bcg) and m. leprae. | thirty-two monoclonal antibodies (moab) to mycobacterium tuberculosis h37rv, m. bovis bcg and m. leprae were produced. the spleen cells of balb/c mice immunized with sonicated or intact bacilli were fused with sp2/0-ag-14 myeloma cells. many more antibody producing hybridomas were found when m. tuberculosis, rather than m. leprae, was used as the immunogen. the moab were characterized by an enzyme immunoassay and immunofluorescence on 16 mycobacterial species. the sodium dodecylsulphate polyacry ... | 1984 | 6439449 |
| skin-test sensitisation by tubercle bacilli and by other mycobacteria in ethiopian school-children. | quadruple skin-testing with a range of 22 new tuberculins and ppd-rt23 was carried out on 665 school-children without bcg scars and 666 with bcg scars, in and around the towns of butajira and hosana in shoa district of ethiopia. marked differences in patterns of sensitisation were distinguished between the 5 schools visited. in general, mycobacterium chitae, m. diernhoferi, m. kansasii and m. vaccae were common sensitising agents in all schools, m. avium subspecies brunense, m. gilvum, m. rhodes ... | 1984 | 6440324 |
| experimental leprosy in nonhuman primates. | 1984 | 6441464 | |
| anti-leprosy vaccines--field trials and future prospects. | development of a vaccine for prevention of leprosy is today one of the major challenges for bio-medical sciences. two vaccines containing (i) m. leprae + bcg and (ii) icrc, a cultivable mycobacterium, have reached advanced stage with reference to human trials. this article describes comparative features of two vaccines and mechanism of their action. future possibilities with reference to anti-leprosy vaccine are also discussed. | 1984 | 6442720 |
| immunological properties of m. leprae culture isolates icrc bacilli: hypothesis on relationship between m. leprae and ml-culture isolates. | the m. leprae-culture isolate, being a culture, is identified by biochemical criteria. majority belong to either m. avium-intracellulare-scrofulacieum complex, or corynobacterium group. the identification is also determined by serological methods using soluble sonicates. skin tests with heat-killed suspension often give positive mitsuda response and hence the isolates are discarded as non-m. leprae. the data on icrc strains show that dharmendra type antigen prepared from icrc bacilli compares ve ... | 1984 | 6442820 |
| further observations on the mycobacteriophage d29-mycobacterial interactions. | 1984 | 6442822 | |
| freeze-fracture observations of the cell walls and peribacillary substances of various mycobacteria. | ultrastructure of the cell wall and peribacillary substances of various mycobacteria (32 strains of 18 species) grown in vitro was studied by a freeze-fracture technique. peribacillary substances differed in shape among species and even among strains of the same species, and were classified into five types: (1) amorphous substances; (2) multi-layered sheaths with no filamentous units; (3) structures composed of filaments of 2-4 nm diameter, which were further classified into three subtypes accor ... | 1984 | 6470664 |
| immunization against leprosy: progress and prospects. | the complex biology of the leprosy organism, the course of the disease, and the atypical human immune response to both vaccination and infection by mycobacterium leprae are reviewed. since the 1960s several trials of vaccination with bcg, the tuberculosis-causing mycobacterium related to the leprosy organism, have showed varied degrees of protection against leprosy. in the 1970s, m. leprae was grown for the first time, in captured wild armadillos, so material is now available for vaccine trial ... | 1984 | 6509251 |
| description of corynebacterium tuberculostearicum sp. nov., a leprosy-derived corynebacterium. | leprosy-derived corynebacteria (ldc) have been extensively studied over the past decade. a composite of their biological properties (cell morphology, staining reactions, cellular inclusions and guanine-plus-cytosine content of their deoxyribonucleic acid; 16 strains studied) and their chemical structures (peptidoglycan type, major cell wall polysaccharide, major glycolipid as well as characteristic mycolic acids) appears to define them as members of the genus corynebacterium. in relation to othe ... | 1984 | 6532280 |
| production and characterization of a murine monoclonal antibody recognizing a shared mycobacterial polysaccharide. | an igm monoclonal antibody specific for mycobacterial arabinomannan was produced by the fusion of splenocytes from balb/c mice immunized with purified arabinomannan with nsi/1 myeloma cells. specificity was demonstrated by gel-radioimmunoassay, and by inhibition of binding using the purified polysaccharide. the monoclonal antibody recognized the arabinomannans from all 18 species of mycobacteria tested, including mycobacterium leprae. this antibody expands the number of defined mycobacterial ant ... | 1984 | 6535819 |
| salivary immunoglobulins and antibody activities in leprosy. | the technics of immunodiffusion and the fluorescent leprosy antibody absorption (fla-abs) test were used to determine the levels of immunoglobulins and their antibody activities against mycobacterium leprae in the serum and the saliva collected from a total of 110 patients with leprosy (50 lepromatous, 24 borderline, and 36 tuberculoid). the average levels of serum igg, igm, and iga were not significantly different among these patients. in saliva, however, igm was detected in only two cases with ... | 1984 | 6541202 |
| ultrastructure of sciatic nerve of armadillo infected with mycobacterium leprae. | ultrastructural observation of sciatic nerves from eight armadillos were made. six animals had intravenous inoculation of m. leprae, one had of foot pad, while one had natural leprosy. the available nerves were biopsied at various time sequence ranging from five weeks to twenty four months. semithin sections did not reveal any neuropathy. ultrastructurally perineurium was thick and endoneurial collagen was increased. initially demyelination of non-myelinated fibres was seen in all nerves irrespe ... | 1984 | 6549323 |
| a test of nonrandom segregation. | within a family, associations between a disease and a marker locus are often inferred when affected offspring share marker alleles more often than is expected by chance. generally, this is due to nonrandom parental transmission of marker alleles and specifically could be due to linkage, epistatic gene action, or segregation distortion at the marker locus. in this paper, we discuss the statistical properties of a general test of nonrandom segregation of a marker gene. the exact probability distri ... | 1984 | 6599403 |
| in vitro effect of mycobacterium leprae suspensions on the polymorphonuclear neutrophils function of hanseniasis patients to candida albicans and candida pseudotropicalis. | the in vitro effect of mycobacterium leprae suspensions on the pmn ability to phagocytizing and killing candida albicans and candida pseudotropicalis was studied in forty-five patients of hansen's disease and in fifteen healthy controls. our results show no significative differences between the different studied groups, both for the phagocytosis and for the lysis of yeasts. there was no significant changes in the mean values of these functions after previous or simultaneously incubation with myc ... | 1984 | 6400664 |
| adoptive cell transfer of resistance to mycobacterium leprae infections in mice. | cells were transferred from mice intradermally vaccinated with killed mycobacterium leprae to sublethally irradiated recipients. unseparated cells from lymph nodes or spleens of m. leprae vaccinated mice were found to cause significant inhibition of the growth of a subsequent m. leprae challenge in mouse footpads for up to 26 weeks after vaccination. vaccination with live bcg and cells transferred from bcg-vaccinated mice caused no significant inhibition of m. leprae growth in mouse footpads. ce ... | 1985 | 3876183 |
| hla-linked control of predisposition to lepromatous leprosy. | in a study of the relation between hla and lepromatous leprosy, hla haplotype segregation was analyzed in 28 families with multiple cases of different types of leprosy. the inheritance of hla-dr2, hla-dr3, and hla-mt1, which had previously been shown to be associated with susceptibility to leprosy or with a leprosy type, was analyzed separately. segregation occurred in a significantly nonrandom fashion in both polar tuberculoid leprosy and lepromatous leprosy. this finding indicated hla-encoded ... | 1985 | 3880800 |
| susceptibility of mycobacterium leprae to the bactericidal activity of mouse peritoneal macrophages and to hydrogen peroxide. | macrophages from athymic nude mice were infected in vitro with mycobacterium leprae to study the intracellular fate of this organism. using the proportional bactericidal test, we have shown that the viability of m. leprae declines rapidly within these macrophages, although results of clearance experiments demonstrate that live and killed organisms are cleared at comparable rates. we have also shown that m. leprae is susceptible to the bactericidal effects of hydrogen peroxide and we suggest that ... | 1985 | 3881593 |
| manifestations of experimental leprosy in the armadillo. | three experiments, using different routes and doses of infection, were conducted using 42 armadillos. thirty-six of them developed generalized disease. there is no significant sex or age difference in susceptibility. route and dose of infection make very little difference in the disease prevalence except that the intravenous administration of a large dose reduces the period of development of generalized disease. it is quite possible that in armadillos the resistance to the disease is partly gene ... | 1985 | 3882008 |
| molecular analysis of dna and construction of genomic libraries of mycobacterium leprae. | molecular analysis of dna from mycobacterium leprae, "mycobacterium lufu," and mycobacterium vaccae has demonstrated that the g + c (guanine plus cytosine) contents of the dnas are 56, 61, and 65%, respectively, and that the genome sizes are 2.2 x 10(9), 3.1 x 10(9), and 3.1 x 10(9) daltons, respectively. because of the significant differences in both g + c content and genome size among m. leprae, "m. lufu," and m. vaccae dnas, these species are not related, although hybridization experiments un ... | 1985 | 3882664 |
| [diagnostic test systems based on the immunoenzyme method in leprosy]. | diagnostic test systems for the detection of igg and igm to mycobacterium leprae in the blood sera of leprosy patients and armadillos experimentally infected with m. leprae have been developed on the basis of the indirect immunoperoxidase assay. the possibility has been shown of prognosing the activity of the leprotic process in leprosy patients and the results of the experimental infection of armadillos by the dynamic increase of antibody reactions with the development of the infection. | 1985 | 3885647 |
| leprosy and the eye: a review. | 1985 | 3886906 | |
| mycobacterium leprae in seminal fluid: a case report. | 1985 | 3887080 | |
| the search for new drugs for the treatment of leprosy. | 1985 | 3887081 | |
| accessory cell function of cells of the mononuclear phagocyte system isolated from mycobacterial granulomas. | epithelioid cells from bcg-induced granulomas and macrophages from mycobacterium leprae-induced granulomas were examined for their ability to act as accessory cells for t-cell proliferation to mitogen (con a) and antigen (ppd). the granuloma cells were separated on a facs using monoclonal antibody specific to guinea pig macrophages. epithelioid cells (which are ia negative) were able to support proliferation to con a but not to antigen. cultures containing ia positive granuloma macrophages from ... | 1985 | 3888411 |
| an attempt to infect turtles (kinosternon leucostonum) with mycobacterium leprae and m. lepraemurium. | 1985 | 3888712 | |
| intracellular multiplication of leprosy-derived mycobacteria in schwann cells of dorsal root ganglion cultures. | organized nerve cultures of dorsal root ganglia from neonatal mice were infected with mycobacterium leprae, the causative agent of leprosy. a significant multiplication of the acid-fast bacilli was observed within the schwann cell component of the culture. the growth of these bacilli was sensitive to antileprosy drugs and was not observed directly in bacteriological media. these organisms were brightly stained with the monoclonal antibody to phenolic glycolipid-i, a m. leprae-specific marker. th ... | 1985 | 3889048 |
| alternate radiolabeled markers for detecting metabolic activity of mycobacterium leprae residing in murine macrophages. | this study demonstrated the utility of using 4% naoh as a murine macrophage cell-solubilizing agent to discriminate between host macrophage metabolism and that of intracellular mycobacterium leprae. a 4% concentration of naoh had no deleterious effect on labeled mycobacteria. thereby, alternate radiolabeled indicators of the metabolic activity of intracellular m. leprae could be experimented with. significant incorporation of 14c-amino acid mixture, [14c]leucine, [14c]uridine, and carrier-free 3 ... | 1985 | 3889052 |
| use of a polysulfone membrane support for immunochemical analysis of a glycolipid from mycobacterium leprae. | polysulfone membranes have been used as a solid support for chromatography and immunoblotting of phenolic glycolipid i from mycobacterium leprae. these membranes have an advantage over other supports such as nitrocellulose and silica gel in that very little non-specific background binding of antibodies occurs and assays can readily be carried out with igm antibodies from human sera. an example of use of the polysulfone chromatography system for detection of phenolic glycolipid i in sera from lep ... | 1985 | 3889165 |
| leprosy in a mangabey monkey--naturally acquired infection. | naturally acquired leprosy was detected in an otherwise normal "sooty" mangabey monkey (cercocebus atys). this animal was imported from west africa in 1975 and developed clinical symptoms of leprosy in 1979. histopathologic findings were those of subpolar-lepromatous to borderline-lepromatous leprosy in the ridley-jopling classification. the disease was progressive, with crippling neuropathic deformities of the hands and feet. the disease regressed under specific therapy. the etiologic agent was ... | 1985 | 3889184 |
| dds-resistant leprosy. | 1985 | 3889186 | |
| drug sensitivity testing of m. leprae. | 1985 | 3889187 | |
| abstracts: first u.s. hansen's disease research conference. national hansen's disease center, carville, louisiana, u.s.a, 13-15 august 1954. | 1985 | 3889188 | |
| serological survey of leprosy and control subjects by a monoclonal antibody-based immunoassay. | a serological antibody competition test (sact) using a murine anti-mycobacterium leprae-specific monoclonal antibody (ml04) has been evaluated in 96 untreated leprosy patients and 128 control subjects. the test is based on the competitive inhibition by antibodies from human test sera of the binding of the specific 125i-ml04 murine monoclonal antibody to m. leprae soluble antigen coated microtiter plates. along the spectrum of the disease, sact positivity was observed in 100% of ll-bl, 87.5% of b ... | 1985 | 3889189 |
| comparative study on the thin-layer chromatographic pattern of methyl mycolates of m. leprae and related species. | the thin-layer chromatographic (tlc) pattern of methyl mycolates of mycobacterium leprae has been compared with the lipids extracted from m. leprae-infected tissue material and also with those of other cultivable mycobacterial species. it has been found that m. leprae-derived methyl mycolates give two spots, namely, alpha- and keto-derivatives, after charring the tlc plates with k2cr2o7/h2so4 spray. lipids extracted from infected tissue material also show spots with the same rf values when compa ... | 1985 | 3889190 |
| an electron microscopic study on macrophages and lymphocytes in lepromatous and borderline leprosy. | we describe how macrophages are activated and phagocytose mycobacteria in lepromatous leprosy. the differentiation of macrophages into epithelioid cells and into giant cells in borderline leprosy is shown. close apposition between macrophages and lymphocytes is seen in those areas where mycobacteria disintegrate inside macrophages. | 1985 | 3889192 |
| an electron microscopic study of the small cutaneous vessels in lepromatous leprosy. | the small dermal vessels play an important role for the propagation of mycobacterium leprae. bacteria can multiply in the endothelial cells as well as in the pericytes and can enter the blood stream from the endothelial cells and the interstitial space from the pericytes. m. leprae can be phagocytosed by blood monocytes. | 1985 | 3889193 |
| electron microscopic findings of the peripheral nerve lesions of nude mice inoculated with m. leprae. | peripheral nerve lesions of nude mice inoculated with mycobacterium leprae were examined from 1 year to 1 year and 8 months post-inoculation. leprosy bacilli and intracytoplasmic foamy structures were found in the perineurial cells, endothelial cells of capillaries, schwann cells, and axons of the myelinated nerve fibers inside the lepromas of foot pads. in the proximal portion of the sciatic nerve, bacilli were found chiefly in the macrophages of the endoneurial space. bacilli were not found in ... | 1985 | 3889194 |
| cultivable mycobacteria isolated from organs of armadillos uninoculated and inoculated with mycobacterium leprae. | mycobacteria were cultivated from 16 out of 32 samples of tissues from armadillos inoculated with mycobacterium leprae. three out of 7 samples from non-inoculated armadillos held in captivity were also positive for cultivable mycobacteria. some isolated strains belonged to the m. avium-intracellulare-scrofulaceum complex, while others were identified as m. gordonae and m. terrae. unclassified mycobacteria were isolated from m. leprae-inoculated armadillos only. taxonomic studies confirmed that t ... | 1985 | 3890695 |
| essential fatty acids in the plasma phospholipids of patients with leprosy. | plasma phospholipid essential fatty acids were investigated in 40 patients with leprosy and 40 controls. a significant reduction in linoleic acid was found in the leprosy patients, with an increase in its metabolite dihomo-gamma-linolenic acid. no difference was found between patients with multibacillary and paucibacillary leprosy. patients treated for less than 6 months were found to have low levels of linoleic acid and high levels of dihomo-gamma-linolenic and arachidonic acid compared with pa ... | 1985 | 3890922 |
| study on the micromorphology of mycobacterium leprae. | the micromorphology of mycobacterium leprae is described. after fixation with osmium tetroxide supplemented with calcium ions, the cell wall was seen to be composed of three layers; the cytoplasmic membrane exhibited the architecture of an elementary membrane. the mesosomes were best visualized after fixation with glutaraldehyde; they were sometimes in contact with the nuclear equivalent. only one sort of phosphate body was found. the nucleoid was best visualized after fixation with osmium tetro ... | 1985 | 3893332 |
| immunochemical characterization of a protein associated with mycobacterium leprae cell wall. | a panel of nine monoclonal antibodies to mycobacterium leprae were used to characterize a protein antigen of the bacillus. two monoclonal antibodies (ivd8 and iiie9) were specific for m. leprae and reacted with an epitope (cwpa) present on a protein molecule associated with the cell wall fraction of m. leprae. this protein, designated cell wall-associated protein (cwp), lost its immunoreactivity upon treatment with trypsin and had an apparent molecular weight of 65,000, though additional lower-m ... | 1985 | 3894233 |
| [osteitis leprosa multiplex cystica--an early symptom of lepromatous leprosy]. | bone lesions of the finger in a 20-year-old leprosy patient are described to show their diagnostic value. the aetiological differences between specific (caused by mycobacterium leprae itself) and unspecific osseal changes are morphologically noticeable in morbus hansen. the x-ray picture shows cystic lightening by granulomatous destruction and distal absorption of the bone. these lesions are typical, and radiological investigations might be the first step in the diagnosis of leprosy. in our case ... | 1985 | 3894289 |
| changes in circulating antibody levels to the major phenolic glycolipid during erythema nodosum leprosum in leprosy patients. | circulating antibody levels to the phenolic glycolipid from mycobacterium leprae and soluble m. leprae antigens were monitored before, during and following enl episodes in 12 patients. it was observed that during enl reaction, there was a fall in circulating antibody levels to the phenolic glycolipid but not to the soluble antigens from m. leprae. when the patients had recovered from their enl reactions, the anti-glycolipid antibody levels usually increased again to levels similar to those obser ... | 1985 | 3894538 |
| phagocytic and bactericidal activities of macrophages from mycobacterium leprae-infected normal and immunosuppressed mice. | phagocytic and bactericidal activities were studied in mycobacterium leprae-infected normal (ni) and thymectomized/irradiated (tri) mice at different time periods. no significant differences were seen in the phagocytic activity for staphylococcus aureus at 3, 6, and 9 months in the normal infected (ni) and normal control (nc) mice. a slight but significant decrease in the phagocytosis of s. aureus was seen in the tri as compared to the ni group at 3 months which recovered at 6 months. phagocytos ... | 1985 | 3894539 |
| electron microscopic findings of transverse fission of m. leprae by freeze-etching methods. | the structures of multiplication by transverse fission found in the lepromas and livers of nude mice and nine-banded armadillos inoculated with mycobacterium leprae isolated from human lepromas, a nine-banded armadillo with naturally acquired leprosy-like disease, and a nine-banded armadillo inoculated with m. leprae isolated from the mangabey monkey with naturally acquired leprosy infection are described. the images of multiplication by transverse fission of m. leprae were almost always found i ... | 1985 | 3894540 |
| an unusual case of untreated polar lepromatous leprosy associated with rare m. leprae. | 1985 | 3894542 | |
| the effect of mycobacterium leprae on pha- and ppd-induced inhibition of leukocyte migration in leprosy patients. | 1985 | 3894846 | |
| rapid, radiometric in vitro assay for the evaluation of the anti-leprosy activity of clofazimine and its analogues. | 1985 | 3894849 | |
| leprosy bacillus outwitted. | 1985 | 3894978 | |
| genes for the major protein antigens of the leprosy parasite mycobacterium leprae. | leprosy, a chronic infectious disease afflicting between 10 and 15 million people, is caused by the obligate intracellular parasite mycobacterium leprae. although m. leprae was the first identified bacterial pathogen of man, basic biochemical, immunological, diagnostic and therapeutic investigations have been severely limited because it remains one of the few human pathogens that have not been cultured in vitro. an m. leprae recombinant dna expression library was constructed to provide a source ... | 1985 | 3894979 |
| isoenzymes of mycobacteria. ii. relevance of ldh zymograms in taxonomy and identification. | the cell free extracts of mycobacteria namely m. kansasii m. avium, m. tuberculosis, bcg (glaxo), m. gastri, m. phlei, m. smegmatis, m. vaccae, m. strain w., m. scrofulaceum, m. gordonae, m. nonchromogenicum e. coli, staph, aureus, and m. leprae infected skin have been electrophoresed and stained for ldh activity. normal skin tissue was also taken as control. it was found that all the organisms tested showed distinct species specific ldh isoenzyme patterns. there was no extra band but an aberran ... | 1985 | 3897403 |
| absence of mycobactin in mycobacterium leprae; probably a microbe dependent microorganism implications. | ferric mycobactins were prepared from mycobacterium phlei. mycobacterium avium--intracellulare a and h, isolated respectively from armadillo and human leprosy specimens. attempts were made to extract mycobactin from host grown m. leprae cells. the crude ferric mycobactin extracts were tested for growth supporting effect on the mycobactin dependent m. paratuberculosis strain atcc 19698. mycobactins prepared from m. phlei and the two m. avium--intracellulare strains had growth promoting effect on ... | 1985 | 3897405 |
| thymopentin treatment in patients with chemotherapy-resistant lepromatous leprosy. | leprosy is a chronic infectious disease caused by mycobacterium leprae; it is chiefly involving the skin and peripheral nerves. in lepromatous leprosy there are widespread loose infiltrates with m. leprae multiplying extensively in the skin macrophages and schwann cells of peripheral nerves. such patients reveal a decrease of circulating t helper cells, which is still more pronounced in the cutaneous lesions. due to the ever increasing bacterial resistance to classical dapsone and combined chemo ... | 1985 | 3898294 |
| detection of phenolic glycolipid i in sera from patients with lepromatous leprosy. | 1985 | 3900237 | |
| results from cation and mass fingerprint analysis of single cells and from atp measurements of m. leprae for drug sensitivity testing: a comparison. | the physiologic states of mycobacterium leprae isolated from patient biopsies were studied using single cell mass spectrometry by laser microprobe mass analysis (lam-ma) and atp bioluminescence assay. the changes in the physiologic state of m. leprae after the patients had been treated with dapsone (dds) monotherapy were also studied. the shift of the low intracellular na+, k+-ratio of untreated m. leprae cells to higher values under dds therapy, as measured from a limited number of single bacte ... | 1985 | 3900243 |
| comparison of radiometric macrophage assay and the mouse foot pad infection for the evaluation of mycobacterium leprae sensitivity/resistance to dapsone. | studies were undertaken in three independent centers to compare the newer, in vitro radiometric macrophage (m phi) assay with the conventional mouse foot pad infection for the diagnosis of dapsone resistance. results obtained on 12 bacilliferous patients showed good concordance in both assays. one strain diagnosed as sensitive in the m phi assay was found to be resistant in the mouse foot pad. three mycobacterium leprae strains considered to be partially resistant in the mouse infection behaved ... | 1985 | 3900244 |