Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification of histidine residues that act as zinc ligands in beta-lactamase ii by differential tritium exchange. | 1. four histidine-containing peptides have been isolated from a tryptic digest of the zn2+-requiring beta-lactamase ii from bacillus cereus. one of these peptides probably contains two histidine residues. 2. the presence of one equivalent of zn2+ substantially decreases the rate of exchange of the c-2 proton in at least two and probably three of the histidine residues of these peptides for solvent 3h. 3. it is concluded that peptides containing at least two of the three histidine residues acting ... | 1979 | 314287 |
| infant foods as a potential source of diarrhoeal illness in rural west africa. | it is common practice in rural areas of the gambia to prepare infant foods in quantities which are sufficient to meet the needs of the day rather than one meal. these are then stored at ambient temperatures for periods up to 12 hours for feeding to the child on demand. the total viable counts and levels of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in 294 infant foods samples from nought to eight hours after preparation. the presence of salmo ... | 1979 | 442188 |
| protection of pregnant mice with phospholipase c and with arvin against foetal death induced by bacterial lipopolysaccharide. | injection of bacterial lipopolysaccharide into pregnant mice resulted in fibrinogen accumulation, thrombosis and haemorrhage in the placental tissue and foetal death. depletion of circulating fibrinogen by a thrombin-like enzyme from the venom of malayan pit viper, arvin, prevents foetal death. foetal protection was also obtained by treating the mothers with a preparation of phospholipase c from bacillus cereus known to inactivate tissue thromboplastin. it is suggested the lipopolysaccharide cau ... | 1979 | 444421 |
| use of enzymatic and electron microscopy (freeze-etching) methods for studying atp-dependent masking of erythrocyte membrane phospholipids. | membrane phospholipids of atp-depleted chicken, rat, and toad erythrocytes are more susceptible than fresh cells to hydrolysis by phospholipase c (bacillus cereus), phospholipase a (bee venom), or the combination of these enzymes and sphingomyelinase. atp depletion of chicken and rat erythrocytes greatly increased the membrane phospholipid fraction, which can be extracted by dry ether. trinitrobenzene sulfonic acid attacked about 20% of the phosphatidylethanolamine of fresh chicken erythrocytes ... | 1979 | 478827 |
| in vitro actions of some antibiotics on phospholipases. | the effects of some antibiotics on activities of phospholipase a2, b and c were investigated in vitro. tetracyclines, macrolides, chloramphenicol and carbenicillin inhibited the activity of crotalus adamanteus phospholipase a2 towards phospholipids of egg-yolk emulsions. when the ability to inhibit the activity of penicillium notatum phospholipase b towards mixed micelles of phosphatidylcholine and triton x-100 was investigated, polymyxin b was found to be inhibitory while chloramphenicol and ca ... | 1979 | 541266 |
| [microfloral study of bull seminal fluid stored at low temperatures]. | hundred twenty three samples of bull semen fluid frozen at 196 degrees c including 83 plastic ampules, 20 granules and 20 plastic straws obtained from the containers of the insemination stations of 10 farms from the sofia district were investigated. two hundred twelve strains were isolated and identified as: escherichia coli--25 strains, hafnia--16 strains, citrobacter, enterobacter and proteus mirabilis--9 strains of each. the remaining gram-negative genera and species were more rarely encounte ... | 1979 | 545852 |
| septicemia in children with cancer. | because of the persistently high mortality from sepsis in cancer patients, a retrospective study was designed to identify the causative organisms and to determine the factors affecting the outcome of sepsis. a total of 84 episodes of septicemia in 61 children with cancer were studied. the more frequently isolated organisms were: staphylococcus aureus (21.4%); escherichia coli (18%); klebsiella (7.1%); pseudomonas (6%); and bacteroides fragilis (6%). other isolates included proteus, serratia, aci ... | 1979 | 371000 |
| the variable response of bacteria to excess ferric iron in host tissues. | the enhancement by exogenous ferric iron, both systemic and local, of the infectivity of 120 strains of bacteria, representing 17 genera, was measured in the skin of guinea-pigs. systemic iron enhanced only 23% of 115 strains, and local iron 49% of 71 strains. systemic iron, by an apparently anti-inflammatory action, depressed the size of lesions produced by 27 of the non-enhanced strains from nine of the genera tested. for most strains, the degree of enhancement was small, ranging from 2- to 8- ... | 1979 | 372534 |
| continuous cultivation for apparent optimization of defined media for cellulomonas sp. and bacillus cereus. | steady-state continuous culture was used to optimize lean chemically defined media for a cellulomonas sp. and bacillus cereus strain t. both organisms were extremely sensitive to variations in trace-metal concentrations. however, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumentation. the optimized media supported critical dilution rates of 0.571 and 0.467 h for cellulomonas and bacillus, respectively. these values ... | 1979 | 16345417 |
| liquid chromatographic determination of dipicolinic acid from bacterial spores. | dipicolinic acid was determined by reverse-phase liquid chromatography. elution was with 0.2 m potassium phosphate, ph 1.8, containing 1.5% tert-amyl alcohol or higher concentrations of lower alcohols or acetonitrile. the normal analytical range was 50 to 1,000 mum, which is equivalent to 0.1 to 1 mg of spores per ml with a relative standard error of 2 to 4% and a detection limit of <100 pmol. dipicolinic acid was fully extracted from spores by heating at ph 1.8 for 10 min at 100 degrees c. spor ... | 1979 | 16345469 |
| influence of environmental factors on antagonism of fungi by bacteria in soil: clay minerals and ph. | the soil replica plating technique was used to evaluate the influence of clay minerals and ph on antagonistic interactions between fungi and bacteria in soil. in general, the antagonistic activity of bacteria towards filamentous fungi was greater in soil than on agar. the spread of aspergillus niger through soil was inhibited by serratia marcescens when the organisms were inoculated into separate sites in soil, and this antagonistic effect was maintained when the soil was amended with 3, 6, 9, o ... | 1979 | 16345477 |
| influence of environmental factors on antagonism of fungi by bacteria in soil: nutrient levels. | the addition of 0.25, 0.5, or 1.0% glucose to a soil (k) amended with either 6% kaolinite (k6k) or montmorillonite (k6m) or the adjustment of the c/n ratio of the soils from 23/1 to 10/1 with nh(4)no(3) eliminated the inhibition of aspergillus niger by serratia marcescens, regardless of whether the fungus and bacterium were inoculated into the same or separate sites in the soils. the adjustment of the c/n ratio to 15/1 or of the c/p ratio from 1,000/1 to 100/1 with kh(2)po(4) did not eliminate t ... | 1980 | 16345509 |
| bacillus cereus infections. | 1980 | 16811117 | |
| bacillus cereus infections. | 1980 | 16811118 | |
| [clofoctol binding by the bacteria (author's transl)]. | clofoctol is an antibacterial agent which is active only on gram + bacteria. the clofoctol binding on bacteria is fast and the number of molecules bound is about 10(6) to 1,5 10(7) molecules per bacteria. bacteria sensitivity towards clofoctol is the result of their ability to bind with it. gram negative and gram + protoplasts of bacteria bind the clofoctol. binding of clofoctol by b. subtilis is obtained with viable cells and also with thermal inactivated cells. binding of clofoctol by the cell ... | 1980 | 6782374 |
| [differentiation of bacilli isolated from raw and pasteurized milk]. | a study was carried out on the species composition, as well as on the biochemical characteristics of 190 strains of aerobic spore-forming microorganisms of the type bacillus, isolated from fresh and pasteurized milk. the differentiation of bacilli was made following petrova's diagram [4]. it was established that the bacillary strains studied referred to i morphological group--bac. licheniformis 55.9%, bac. subtilis 24.8%, bac. puminis 14.7% and bac. cereus 4.6%. the results of the experiments th ... | 1980 | 6782749 |
| analysis of biological thiols: derivatization with monobromotrimethylammoniobimane and characterization by electrophoresis and chromatography. | 1980 | 6449160 | |
| purification and characterization of a dna-dependent atpase from bacillus cereus. | a dna-dependent atpase (molecular weight 71 000) free of nuclease activity has been purified from bacillus cereus. the enzyme shows similar characteristics as the enzyme isolated from escherichia coli and bacillus subtilis. heat denatured dna stimulates the rate of atp hydrolysis to adp and pi to an extent about tenfold higher than the native dna. double stranded dna without single stranded regions is not a suitable cofactor for the enzyme. the atpase is inhibited by adenosine 5'-(beta, gamma-im ... | 1980 | 6451147 |
| antimicrobial activity of street heroin. | street heroin and injection paraphernalia have been implicated as sources of bacteria causing infections in drug abusers [1]. staphylococcus aureus, pseudomonas aeruginosa, and bacillus cereus are common etiologic agents. in a previous study of the microbiology of street heroin and injection paraphernalia, bacillus species was the predominant isolate [2]. we did not find s. aureus, but one study reported isolates of identical phage type from heroin powder and from an infected patient [3]. to rec ... | 1980 | 6780635 |
| a simple purification scheme yielding crystalline phospholipase c from bacillus cereus. | a very simple and rather unusual purification scheme for phospholipase c from bacillus cereus has been worked out. air is bubbled vigorously through the bacterial culture and the foam collected. liquified foam is centrifuged, dialyzed and heated at 74 degrees c for 5 min. after centrifugation, affinity chromatography is carried out on lipoprotein-sepharose. the enzyme is then thermally denatured by exposure to 85 degrees c for 5 min and the precipitated material well washed and then renatured fr ... | 1980 | 6781184 |
| production of a variant of beta-lactamase ii with selectively decreased cephalosporinase activity by a mutant of bacillus cereus 569/h/9. | 1. mutants of bacillus cereus 569/h/9 have been screened in a search for strains that synthesize variants of beta-lactamase ii. 2. one of these mutants (strain 569/h/9/1) produces a beta-lactamase ii-like enzyme that shows a selective decrease in cephalosporinase activity. 3. beta-lactamase ii from strain 569/h/9/1 has been purified to apparent homogeneity and its kinetic properties have been examined. this enzyme resembles the parent beta-lactamase ii in its relative activity with benzylpenicil ... | 1980 | 6781486 |
| phenylpropynal, a specific, irreversible, non-beta-lactam inhibitor of beta-lactamases. | phenylpropynal is a specific, irreversible, non-beta-lactam inhibitor of typical beta-lactamases. in the presence of millimolar concentrations of phenylpropynal, the beta-lactamase i of bacillus cereus and the beta-lactamases of staphylococcus aureus and escherichia coli become completely inactivated; the beta-lactamase ii of b. cereus is not affected. the e. coli beta-lactamase is considerably more sensitive to the reagent than the gram-positive enzymes. a variety of structural analogs of pheny ... | 1980 | 6765945 |
| atp-sensitive ribonuclease of bacillus cereus. | 1980 | 6766142 | |
| studies on the heat resistance of bacillus cereus spores and growth of the organism in boiled rice. | a comparison was made of the heat resistance of bacillus cereus spores at 95 degrees c. spores of serotype 1 strains were more resistant than those of the other types tested. however, there was little difference in the growth rate of the various serotypes in boiled rice at 22 degrees c. most samples of uncooked rice contained multiple serotypes of b. cereus. these results indicate that the cooking procedure used for the preparation of cooked rice is likely to be selective for certain serotypes, ... | 1980 | 6766156 |
| bacillus cereus autolytic endoglucosaminidase active on cell wall peptidoglycan with n-unsubstituted glucosamine residues. | an autolytic glycosidase from a lysozyme-resistant strain of bacillus cereus capable of cleaving the glycosidic linkages of n-unsubstituted glucosamine in the cell wall peptidoglycan was studied. this glycosidase activity, together with n-acetylmuramyl-l-alanine amidase activity, was found in an autolytic enzyme preparation obtained from the 20,000 x g precipitate fraction by means of autolysis followed by ammonium sulfate fractionation. the major saccharide fragments resulting from digestion of ... | 1980 | 6766437 |
| the crystalline layers in spores of bacillus cereus and bacillus thuringiensis studied by freeze-etching and high resolution electron microscopy. | the comparative morphology of spores from bacillus cereus, b. thuringiensis s-9 (wildtype), b. thuringiensis hb 9-1 (acrystaliferous mutant) and b. finitimus, was studied by the freeze-etching technique. particular attention was given to the three crystalline layers found in these spores, the pitted layer, the parasporal layer, and the basal layer of the exosporium with lattice constants of 9.2 nm, 5.8 nm and 8.0 nm respectively. the parasporal layers, corresponding to the fraction of fl of sche ... | 1980 | 6766865 |
| meningitis and bacteremia; induced by bacillus cereus. | 1980 | 6767208 | |
| panophthalmitis due to bacillus cereus. | a rapidly progressing panophthalmitis due to bacillus cereus developed in three patients. infection was associated with intravenous drug abuse in two patients and was traced to contaminated injection paraphernalia in one. in the third patient, infection was associated with a foreign-body injury to the eye. anterior chamber aspiration revealed the organism on gram's stain in one case and isolation of the bacteria in all three. despite intravenous and intraocular antibiotic therapy, the infection ... | 1980 | 6767460 |
| a bacillus cereus mutant defective in spore coat deposition. | spores of bacillus cereus mutants selected for slow response to germinants and sensitivity to lysozyme were found to be deficient in coat, but were heat-resistant and contained the same quantity of dipicolinic acid as the wild-type. while the average coat protein content of the spores was 25% of the wild-type, many spores were coatless with large whorls of coat deposited in the cytoplasm. these coat deposits were isolated in renografin gradients and found to cross-react immunologically with wild ... | 1980 | 6767805 |
| plasmid-mediated transformation in bacillus megaterium. | a transformation system was developed for bacillus megaterium by using antibiotic resistance plasmid deoxyribonucleic acid molecules derived from staphylococcus aureus and bacillus cereus. lysozyme-generated protoplasts of b. megaterium allowed uptake of plasmid deoxyribonucleic acid in the presence of polyethylene glycol. transformants expressed the antibiotic resistance determinants present on the plasmid deoxyribonucleic acid, and reisolated plasmid deoxyribonucleic acid yielded restriction e ... | 1980 | 6769902 |
| rapid isolation of bacteria from septicemic patients by use of an antimicrobial agent removal device. | the new antimicrobial removal device increased the efficacy of conventional methods for isolating bacteria from the blood of septicemic patients. the device removes as much as 100 microgram of antibiotics per ml from whole blood without a significant decrease in bacteria. of 51 patients studied, 31 yielded positive cultures, and the antimicrobial removal device permitted earlier isolation of the causative agent from blood. subcultured within the first 12 h yielded 12 positive isolates, as the 31 ... | 1980 | 6769953 |
| bacillus cereus infections. | 1980 | 6769974 | |
| [aerobic sporiferous bacteria as causative agents of inflammation]. | 1980 | 6770318 | |
| the isoelectric point of phospholipase c from bacillus cereus. | 1980 | 6771157 | |
| the hydrolysis of sphingomyelin by phospholipase c from bacillus cereus. | 1980 | 6771160 | |
| delayed germination of bacillus cereus t spores after treatment with trichloroacetic acid and their reactivation by heating. | treatment of bacillus cereus t spores with trichloroacetic acid delayed their germination. the extent of retardation depended on the concentration of trichloroacetic acid, and the temperature, ph and duration of treatment. the effect was completely reversed by subsequent heating, and this restoration of germination also depended on the temperature and duration of heat treatment. fourteen compounds were examined for their ability to suppress germination of spores. the halogenated fatty acids test ... | 1980 | 6771492 |
| measurements of the ph within dormant and germinated bacterial spores. | the ph within the core or central region of dormant spores of bacillus cereus and b. megaterium is 6.3-6.4 irrespective of the external ph. however, the spore's internal ph rises to 7.3-7.5 upon germination. the low internal ph of the dormant spore may be a contributing factor to its metabolic dormancy. | 1980 | 6771755 |
| bacillus cereus bacteremia and hemolytic anemia in a patient with hemoglobin sc disease. | a patient with hemoglobin sc disease and cholelithiasis was found to have bacillus cereus bacteremia. hemolytic anemia developed, for which common causes of hemolysis were excluded, suggesting a relationship with the bacteremia. following in vitro incubation, type o erythrocytes were hemolyzed by the culture, but not by a bacteria-free filtrate. this case confirms the association between sickle cell disorders and cholelithiasis with b cereus infections. in addition, it provides evidence for in v ... | 1980 | 6772119 |
| certain monocyclic beta-lactams are beta-lactamase substrates: nocardicin a and desthiobenzylpenicillin. | 1980 | 6772177 | |
| evidence of thermonuclease production by bacillus spp. and enterococci in naturally contaminated cheese. | of 105 thermonuclease-positive (tnase-positive) cheese samples comprising 13 types, 92 (87.6%) contained coagulase-positive staphylococci, whereas 9 (8.6%) contained microorganisms other than staphylococci as the major contaminants. of the latter group, six samples contained bacillus spp. comprising three species (b. cereus, b. licheniformis, and b. subtilis), and three contained mainly enterococci (streptococcus faecalis), which were proven to be tnase producers. the organisms responsible for t ... | 1980 | 6772291 |
| effect of n-acyl substitution at glucosamine residues on lysozyme-catalyzed hydrolysis of cell-wall peptidoglycan and its oligosaccharides. | 1980 | 6772447 | |
| heat stability of bacillus cereus enzymes within spores and in extracts. | inactivation rates for nine enzymes extracted from bacillus cereus spores were measured at several temperatures, and the temperature at which each enzyme had a half-life of 10 min (inactivation temperature) was determined. inactivation temperatures ranged from 47 degrees c for glucose 6-phosphate dehydrogenase to 70 degrees c for leucine dehydrogenase, showing that spore enzymes were not unusually heat stable. enzymes extracted from vegetative cells of b. cereus had heat stabilities similar to t ... | 1980 | 6772628 |
| distribution of calcium and other elements in cryosectioned bacillus cereus t spores, determined by high-resolution scanning electron probe x-ray microanalysis. | the distribution of a number of key elements in bacillus cereus t spores was determined by high-resolution scanning electron probe x-ray microanalysis. to circumvent the redistribution of soluble or weakly bound elements, freeze-dried cryosections of spores, which had been rapidly frozen in 50% aqueous polyvinyl pyrrolidone, were employed. the sections were examined by using a modified philips em400 electron microscope fitted with a field emission gun, scanning transmission electron microscopy a ... | 1980 | 6772633 |
| lysis of erythrocytes from stored human blood by phospholipase c (bacillus cereus). | the ability of phospholipase c (bacillus cereus) to lyse erythrocytes from human blood that had been stored under transfusion service conditions for up to 16 weeks has been examined. when incubated at 20 degrees c with enzyme (0.03 mg/ml, 55 units/ml) for up to 1 h fresh erythrocytes were not lysed. after about 4 weeks of storage a population of very readily lysed erythrocytes appeared. the morphological changes in erythrocytes from blood stored up to 16 weeks were examined by scanning electron ... | 1980 | 6773524 |
| inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopencillanic acid: mechanism. | the mechanism of the inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid, a probable suicide substrate [see loosemore, m.j., cohen, s.a., & pratt, r.f. (1980) biochemistry (preceding paper in this issue)], is described. inactivation is accompanied by covalent modification of the protein with the appearance of a characteristic chromophore at 326 nm. ultraviolet (uv) absorption, nuclear magnetic resonance (nmr), and circular dichroic (cd) spectra of the modified prote ... | 1980 | 6773559 |
| mechanism of substrate-induced inactivation of beta-lactamase i. | beta-lactamase i (from bacillus cereus 569/h) is inactivated by certain substrates (e.g. methicillin or cloxacillin) but not by others (e.g. benzylpenicillin). emzyme that had been inactivated was found to be labelled stoichiometrically, as shown by the use of radioactive methicillin. use of the penamaldate reaction showed the presence of a penicilloyl group in the enzyme inactivated by either methicillin or cloxacillin. in conditions under which enzymic activity was regained the penicilloyl gro ... | 1980 | 6773776 |
| growth of bacillus cereus in media containing plant seed materials and ingredients used in chinese cookery. | 1980 | 6773916 | |
| effect of high concentrations of carbon dioxide on growth rate of pseudomonas fragi, bacillus cereus and streptococcus cremoris. | 1980 | 6773917 | |
| [preliminary study of bacterial resistance to antibiotics isolated from food products]. | 1980 | 6774403 | |
| enzymatic deacetylation of n-acetylglucosamine residues in cell wall peptidoglycan. | an enzyme which catalyzes the hydrolysis of acetamido groups of n-acetylglucosamine residues in cell wall peptidoglycan was found in the supernatant and 20,000 x g pellet fractions of bacillus cereus. autolysis of the latter fraction resulted in solubilization and activation of the deacetylase. among various bacteria, strains of b. cereus which contain high proportions of n-unsubstituted glucosamine residues in their cell wall peptidoglycan components are particularly rich in the deacetylase. th ... | 1980 | 6774970 |
| bacterial debridement of the burn eschar: the in vivo activity of selected organisms. | 1980 | 6775153 | |
| structure of linkage region between glycerol teichoic acid and peptidoglycan in bacillus cereus ahu 1030 cell walls. | 1980 | 6775628 | |
| enumeration and confirmation of bacillus cereus in foods: collaborative study. | a collaborative study was conducted in 15 laboratories to evaluate 2 different techniques for enumerating bacillus cereus in foods. a direct plating technique using mannitol-egg yolk-poly-myxin agar and a most probable number (mpn) technique using trypticase-soy-polymyxin broth were compared for the enumeration of high and low populations of b. cereus in mashed potatoes. the collaborative results showed that the overall mean recovery obtained with the low population level was essentially the sam ... | 1980 | 6776089 |
| endophthalmitis due to bacillus cereus: case report. | an acute case of endophthalmitis following ocular trauma involving barbary branches is presented. bacillus cereus var. mycoides was cultured from lens material obtained via a limbal incision. | 1980 | 6776450 |
| [comparison of culture media for quantitative determination of bacillus cereus in various food products]. | 1980 | 6776617 | |
| cardiolipin, a major phospholipid of gram-positive bacteria that is not readily extractable. | extraction of phospholipids from stationary phase grown cells of the gram+ bacteria, bacillus megaterium, bacillus subtilis, bacillus cereus and micrococcus lysodeikticus was found to be incomplete with various commonly used extraction procedures. phosphatidylglycerol and phosphatidylethanolamine were readily extracted but up to 95% of the cardiolipin appeared to be retained within the cell residue. extraction of the cardiolipin could be slightly enhanced by increasing the temperature or the aci ... | 1980 | 6776994 |
| [regulation of the biosynthesis of branched aminoacyl trna synthetases in bacillus cereus t]. | the regulation of the biosynthesis of isoleucyl-, valyl-, and leucyl-trna synthetases was studied with an isoleucine-valine requiring mutant of bacillus cereus t. it was shown that valyl-trna synthetase regulation is under multivalent control involving both valine and isoleucine. the isoleucyl- and leucyl-trna synthetases are repressed by the respective cognate amino acid. when two amino acids were removed from the culture medium, derepression of the two corresponding aminoacyl trna synthetases ... | 1980 | 6778511 |
| separation and characterization of an autolytic endo-beta-glucosaminidase from bacillus cereus. | 1. an autolytic endo-beta-glucosaminidase, capable of cleaving the glycoside linkages of n-unsubstituted glucosamine in the glycan moiety of cell wall peptidoglycan, was purified 470-fold from a salt extract of the 2,000 x g precipitate fraction obtained after sonication of a lysozyme-resistant strain of bacillus cereus. the properties of this enzyme were studied. 2. the purified enzyme preparation was also active towards the glycan chain of fully n-acetylated cell wall peptidoglycan. 3. the end ... | 1980 | 6780345 |
| the occurrence of bacillus cereus in some dried foods including pulses and cereals. | 1980 | 6780506 | |
| [biosynthesis of extracellular phospholipase c (lecithinase) from bacillus cereus depending on the nutrient medium composition and ph]. | the nutrient medium to provide rapid growth of microbial cells of bacillus cereus str. 504, and biosynthesis of extracellular phospholipase c(ec 3.1.4.3) was selected. the nutrient medium contained acidic casein hydrolyzate, yeast extract or enzymatic hydrolyzate of fodder yeast, glucose, nacl, nahco3, and na2hpo4. the activity of secreted phospholipase c reached maximum at ph 6.3--6.6. | 1980 | 6784112 |
| dna-dependent atpase ii from bacillus cereus. | a new dna-dependent atpase been purified close to homogeneity from soluble extracts of bacillus cereus. this enzyme, called atpase ii catalyses the hydrolysis of atp in the presence of mg2+ or ca2+ and dna. single-stranded linear dna is a cofactor about 3-fold more effective than double-stranded dna. the enzyme catalyses the strand separation of duplex dna in the presence of atp. however, at concentrations higher than 0.5 mm, phosphohydrolysis can occur without concomitant dna unwinding. the enz ... | 1980 | 6107127 |
| the structure of beta-lactamases. | the beta-lactamases are widely distributed in both gram-positive and gram-negative bacteria. they all inactivate penicillins and cephalosporins by opening the beta-lactam ring. many varieties of the enzyme can be distinguished on the basis of their catalytic and molecular properties, but only amino acid sequence determination gives information upon which a molecular phylogeny can be based. the present evidence suggests that the beta-lactamases have a polyphyletic origin. all the beta-lactamases ... | 1980 | 6109327 |
| active sites of beta-lactamases from bacillus cereus. | there are two extracellular beta-lactamases produced by bacillus cereus 569. one of these enzymes, beta-lactamase i, is inactivated by 6-beta-bromopenicillanic acid: the site of reaction is serine-44. this is a conserved amino acid residue in the other beta-lactamases whose structures have been determined, and it becomes a good candidate for an active-site group in these enzymes. the inactivation may involve a rearrangement leading to a dihydrothiazine. the other extracellular enzyme produced by ... | 1980 | 6109328 |
| preferential suppression of normal exoenzyme formation by membrane-modifying agents. | cerulenin, phenethyl alcohol, benzyl alcohol, procaine, and a series of aliphatic alcohols selectively suppressed production of active exoenzymes by various bacterial strains. | 1980 | 6154046 |
| the mode of action of nanaomycin a in gram-positive bacteria. | the mode of action of nanaomycin a on gram-positive such as staphylococcus aureaus, bacillus cereus and streptococcus faecalis was investigated. nanaomycin a inhibited the biosyntheses of protein, dna, rna and cell-well peptidoglycan to a similar extent. it increased the oxygenous respiration of s. aureus cells at the minimal inhibitory concentration. the cells preincubated with nanaomycin a showed stimulation of proton influx after addition of n,n'-dicyclo-hexylcazrbodiimide, an inhibitor of ca ... | 1980 | 6159343 |
| phospholipase c (bacillus cereus) digestion of phospholipids in myelin isolated from central nervous tissue: removal of myelin basic protein increases exposure of lipids to the enzyme. | 1980 | 6161042 | |
| commercial milk products and indigenous weaning foods in a rural west african environment: a bacteriological perspective. | two commercially available baby milks, one 'biologically acidified', the other 'non-acidified', and a traditional weaning food, millet gruel, were prepared and stored under village conditions in west africa. increases in total colony count and in number of bacillus cereus, clostridium welchii, staphylococcus aureus and escherichia coli were determined in these products when stored as commonly practised at ambient temperatures over a period of 8 h. poor hygiene during preparation was indicated by ... | 1980 | 6244343 |
| studies on sphingomyelinase of bacillus cereus: hydrolytic and hemolytic actions on erythrocyte membranes. | 1980 | 6244783 | |
| rapid spectrophotometric method for the determination of beta-lactamase activity. | 1980 | 6247317 | |
| cross-linking preserves conformational changes induced in penicillinase by its substrates. | exopenicillinase of bacillus cereus 569/h was cross-linked with toluene 2,4-diisocyanate in the presence of cephalothin, cloxacillin or no substrate. the derivatives show significant differences in susceptibility to inactivation by heat, urea, iodination or proteolysis. such differences can be predicted from the contrasting effects of these substrates on the conformation of the enzyme. | 1980 | 6249271 |
| spectrophotometric determination of inhibitory effects of cp-45899 on beta-lactamase with benzylpenicillin and nitrocefin. | 1980 | 6249784 | |
| inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid: kinetics. | the kinetics of the inactivation of bacillus cereus beta-lactamase i by 6 beta-bromopenicillanic acid are described. loss of beta-lactamase activity is accompanied by a decrease in protein fluorescence, by the appearance of a protein-bound chromophore at 326 nm, and by loss of tritium from 6 alpha-[3h]-6 beta-bromopenicillanic acid. it is shown that all of the above changes probably have the same rate-determining step. the inactivation reaction is competitively inhibited by cephalosporin c, a co ... | 1980 | 6250581 |
| induction of accelerated sporangial lysis by basic peptide antibiotics. a novel method of preparation of free spores of bacilli. | 1980 | 6253430 | |
| on the chemistry of beta-lactamase inhibition by 6 beta-bromopenicillanic acid. | 1980 | 6253512 | |
| behavior of pathogenic bacteria in the oyster, crassostrea commercialis, during depuration, re-laying, and storage. | oysters (crassostrea commercials) harvested from major cultivation areas within the state of new south wales, australia, were commonly contaminated with low levels of the food-poisoning organisms bacillus cereus, clostridium perfringens, and vibrio parahaemolyticus. salmonella was found in oysters on only one occasion. these bacteria were cleansed from oysters during oyster purification by re-laying in a non-polluted waterway. oysters were laboratory contaminated to levels in excess 1,000 cells ... | 1980 | 6257164 |
| [food bacteriology in madagascar. laboratory activities from 1965 to 1977. techniques and results]. | 1980 | 6263201 | |
| [microbial flora of beef extract imported from australia, uruguay, italy and brazil (author's transl)]. | 1980 | 6265014 | |
| immunological detection of food-poisoning toxins. | 1980 | 6269224 | |
| kinetics of inactivation of beta-lactamase i by 6 beta-bromopenicillanic acid. | the kinetics of the inactivation of beta-lactamase i from bacillus cereus 569 by preparations of 6 alpha-bromopenicillanic acid showed unexpected features. these can be quantitatively accounted for on the basis of the inactivator being the epimer, 6 beta-bromopenicillanic acid. at ph 9.2, the rate-determining step in the inactivation is the formation of the inactivator. when pure 6 beta-bromopenicillanic acid is used to inactivate beta-lactamase i, simple second-order kinetics are observed. the ... | 1980 | 6331385 |
| the 1h nuclear-magnetic-resonance spectroscopy of cobalt(ii)-beta-lactamase ii. | the 1h n.m.r. spectra of beta-lactamase ii in the presence of co(ii) were studied. analysis of the spectra suggests that co(ii) binds at the same two metal-binding sites as does zn(ii). the binding of co(ii) at the first site is much weaker than the binding of zn(ii) at this site, whereas the binding of co(ii) at the second site is tighter than the binding of zn(ii). the binding of co(ii) to the mono-zinc(ii)-enzyme caused only one marked change in the spectrum, namely a decrease in the intensit ... | 1980 | 6821370 |
| properties of an acid phosphatase in pulmonary surfactant. | lung surfactant, a lipid-protein complex purified from dog lungs, contains a highly active phosphomonoesterase associated with it. this phosphatase is quite specific for the hydrolysis of phosphatidic acid and 1-acyl-2-lysophosphatidic acid. the enzyme possesses many of the characteristics of the microsomal enzyme, phosphatidate phosphohydrolase (ec 3.1.3.4). in addition, we have shown that this enzyme will also convert phosphatidylglycerol phosphate [1-(3-sn-phosphatidyl)-sn-glycerol-1-p] to ph ... | 1980 | 6928679 |
| conjugation of enzymes to immunoglobulins using dimaleimides. | a method is described for coupling enzymes to immunoglobulins using sulphydryl derivatives of the proteins and a dimaleimide which is relatively water-soluble. parameters affecting the performance of the conjugates have been examined including level of sulphydryl incorporation, ratio of enzyme/immunoglobulin and nature of dimaleimide used. peroxidase-immunoglobulin conjugates made by the dimaleimide method have been compared with those made by the periodate oxidation method and found to have a s ... | 1980 | 6988011 |
| [lipase and phospholipase from staphylococcus aureus of different origin. ii. purification and characterization (author's transl)]. | lipase and phospholipase c from staphylococcus aureus could be isolated by gel filtration on sephacryl s 200 (fig. 1a, b) and completely separated by refiltration under the same conditions. isoelectric focusing gave maximal enzyme-activities for lipase at ph 8.6 and 9.5 and for phospholipase c at ph 7.4 (fig. 2). thin-layer chromatography revealed that the reaction products in lecithin agar of the phospholipase c-preparations from s. aureus and bacillus cereus were identical (table 1). | 1980 | 7223122 |
| generalized transduction in bacillus thuringiensis var. berliner 1715 using bacteriophage cp-54ber. | a phage isolated from lysates of phage cp-54 grown on bacillus cereus 569 and selected on the basis of its ability to infect bacillus thuringiensis var. berliner 1715 (serotype i) was designated cp-54ber. phages cp-54ber and cp-54 were similar in size, morphology, cryosensitivity and stabilization by dimethyl sulphoxide. they showed significant differences with regard to inactivation by specific antiserum, adsorption to the berliner strains and host range. phage cp-54ber was able to mediate gene ... | 1980 | 7252480 |
| a novel n-acetylglucosaminyl polyprene in hen oviduct. | incubation of hen oviduct membranes with udp-[14c]n-acetylglucosamine (glcnac) leads to the formation of four endogenously labeled lipids. one of these lipids has not previously been characterized. based on its chromatographic behavior on sg-81 paper and deae-cellulose, it is a monophosphate diester. the oviduct lipid is extremely sensitive to mild acid hydrolysis and, based on a comparison with alpha-glcnac-phosphoryl-undecaprenol from bacillus cereus, the oviduct lipid contains glcnac linked t ... | 1980 | 7400130 |
| composition and properties of the cell wall of methanospirillum hungatii. | dithiothreitol reacted, at ph 9.0, with the isolated cell walls of methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. sugars were identified as rhamnose, ribose, glucose, galactose, and mannose. the wall material that was released contained only 47% amino acids and was enriched in lipid, glucose, and phosphate. these results support ... | 1980 | 7407700 |
| ageing of blood in hereditary spherocytosis. | blood from 5 patients suffering from hereditary spherocytosis (hs) was stored in acid-citrate-dextrose anticoagulant at 4 degrees c for up to 6 weeks. the erythrocyte morphology and susceptibility to lysis by phospholipase c (bacillus cereus) were examined at 2-weekly intervals and compared with erythrocytes from 6 different donations of stored normal blood. the hereditary spherocytes went through essentially the same series of morphological changes as did normal erythrocytes. fresh hereditary s ... | 1980 | 7466302 |
| bacteriological examination of ice-cream in the netherlands: comparative studies on methods. | 1980 | 6783602 | |
| a simple and rapid computer-assisted technique for the identification of some selected bacillus species using biochemical tests. | 1980 | 6783604 | |
| influence of curvature of response lines in antibiotic agar diffusion assays. | 1981 | 6783664 | |
| glysperin, a new antibiotic complex of bacterial origin. i. production, isolation and properties. | strains of bacillus cereus produced a complex of new antibiotics, glysperins a, b and c. they are basic, water-soluble antibiotics and active against gram-positive and gram-negative bacteria including aminoglycoside-resistant organisms. glysperin a is a major component of the antibiotic complex and approximately two to four times more active than components b and c. | 1981 | 7275817 |
| the specificity requirements of bacteriophage t4 lysozyme. involvement of n-acetamido groups. | bacillus cereus peptidoglycan with n-unsubstituted glucosamine residues was insensitive to treatment with bacteriophage t4 lysozyme. after n-acetylation with acetic anhydride, t4 lysozyme cleared solutions of the peptidoglycan and reducing sugars were liberated. the digestion products were mainly of high molecular weight, since the peptidoglycan is peptide cross-linked to a great extent. n-propylation did not convert the partially n-unsubstituted peptidoglycan to a sensitive form. it is conclude ... | 1981 | 7308203 |
| [investigations on the sensitivity of the detection of chlortetracycline (ctc) in blood and medicated feed within the official prophylaxis and therapy of psittacosis, using the bacillus cereus-inhibition-test in agar-plates (author's transl)]. | 1981 | 7016496 | |
| cholesterol solubilization by short-chain lecithins: characterization of mixed micelles and cholesterol oxidase activity. | the synthetic short-chain lecithins diheptanoylphosphatidylcholine and dioctanoylphosphatidylcholine solubilize cholesterol up to 10 and 18 mol %, respectively. the half-time for diheptanoylphosphatidylcholine solubilization of solid cholesterol is 80 (+/- 30) min. this is much faster than triton x-100 micelle or egg lecithin vesicle solubilization of solid cholesterol. both the broadening of lecithin and [4-13c]cholesterol carbon resonances by mn2+ and the observation of surface dilution kineti ... | 1981 | 6947824 |
| properties of ro 13-99041 as a substrate and inhibitor of beta-lactamases. | ro 13-9904 is a novel semisynthetic and highly active parenteral cephalosporin. its stability against hydrolysis by several beta-lactamases was studied. the enzymes were isolated from various enterobacteriaceae or staphylococcus aureus and several commercially available enzyme preparations were also included. most of the penicillinases, cephalosporinases or the tem-type beta-lactamase studied were unable to hydrolyze this novel cephalosporin. the cephalosporinases from bacillus cereus 569/h9 and ... | 1981 | 6972858 |
| interaction of beta-lactamase of streptomyces cacaoi. i. clavulanic acid and ps-5. | inactivation of a beta-lactamase of streptomyces cacaoi by clavulanic acid and ps-5 was investigated and compared with that of a beta-lactamase of bacillus cereus. inhibition of the enzymes induced by clavulanic acid and the beta-lactam antibiotic ps-5 was found to be progressive with time. however, the degree of inhibition of the beta-lactamase from s. cacaoi increased more progressively with time than that of the enzyme from b. cereus. conformative response constants were determined. as compar ... | 1981 | 6975771 |
| bacillus cereus cross-infection in a maternity-unit. | 1981 | 6175691 | |
| food borne disease. | 1981 | 6271107 | |
| studies on (k+ + h+)-atpase. iv. effects of phospholipase c treatment. | (1) the total phospholipid content of a gradient purified (k+ + h+)-atpase preparation from pig gastric mucosa is 105 mumol per 100 mg protein, and consists of 29% sphingomyelin, 29% phosphatidylcholine, 28% phosphatidylethanolamine, 10% phosphatidylserine and 4% phosphatidylinositol. the cholesterol content corresponds to 50 mumol per 100 mg protein. (2) treatment with phospholipase c (from clostridium welchii and bacillus cereus) results in an immediate decrease of the phosphate content. up to ... | 1981 | 6272855 |
| interaction of beta-lactamase of streptomyces cacaoi. ii. cp-45,899, izumenolide and cephamycins. | inhibition of a beta-lactamase of streptomyces cacaoi by cp-45,899, izumenolide and cephamycins was investigated and compared with that of a beta-lactamase of bacillus cereus. s. cacaoi enzyme could not hydrolyze cp-45,899. instead, hydrolysis of benzylpenicillin by the enzyme was inhibited in the presence of cp-45,899. although inhibition increased gradually with time, the inhibition line produced by cp-45,899 with time less curved than that produced by clavulanic acid and ps-5. furthermore, pr ... | 1981 | 6273376 |