Publications

comparative analysis of the four rrna operons in finegoldia magna atcc29328.	there are four rrna operons rrna, rrnb, rrnc and rrnd on the genome of finegoldia magna (formerly peptostreptococcus magnus) atcc29328, which, in contrast to those of clostridia, are dispersed around the chromosome. using a bac library we determined the nucleotide sequences and structures of all four operons, including their flanking regions, and performed comparative analyses. we identified putative boxa sequences in the operons, which should be required for rrna transcription antitermination, ...	2004	15053317
septic arthritis of the hip and intrapelvic abscess following intra-articular injection of hylan g-f 20. a case report.		2004	15069151
anaerobic pulmonary infections in children.	pulmonary infections due to anaerobic bacteria usually occur in children prone to aspiration. the source of the anaerobic bacteria is the oropharyngeal bacterial flora, where these organisms outnumber aerobic and facultative organisms in a 10:1 ratio. the most common lower respiratory tract infections where anaerobic bacteria are recovered mixed with aerobic organisms are aspiration pneumonia, lung abscess, and empyema. the predominant isolated anaerobic bacteria are peptostreptococcus, fusobact ...	2004	15599270
[treatment of acute pyoinflammatory diseases of the lungs and pleura under control of microorganism persistence factors].	results of treatment of patients with acute pulmonary abscess, acute empyema of the pleura and pyopneumothorax were analyzed. the local treatment of the main group of patients consisting of 50 persons included oxitocin in combination with antiseptics and antibiotics which facilitated maximal reduction of the persistent potential of the pathogenic agent, its rapid elimination from the purulent focus and allowed to get better results using conservative therapy. the obtained clinical effect confirm ...	2004	15626066
[clinical and bacteriological substantiation of the use of cefoperazone/sulbactam in complex therapy of patients with pyo-destructive forms of lower respiratory tract infection (lrti)].	antibacterial therapy is the most important component of the complex management of pyo-destructive forms of lrti. since the microbial flora is rather variable and polymorphous, antibiotics used in the treatment of lrti should be active against both aerobic (especially gramnegative) and anaerobic pathogens. the aim of the study was to prove, on the basis of the bacteriological and clinical findings, the validity of the use of cefoperazone/sulbactam (cs), the only inhibitor-protected cephalosporin ...	2004	15628799
estimates of methyl 13c and 1h csa values (deltasigma) in proteins from cross-correlated spin relaxation.	simple pulse schemes are presented for the measurement of methyl (13)c and (1)h csa values from (1)h-(13)c dipole/(13)c csa and (1)h-(13)c dipole/(1)h csa cross-correlated relaxation. the methodology is applied to protein l and malate synthase g. average (13)c csa values are considerably smaller for ile than leu/val (17 vs 25 ppm) and are in good agreement with previous solid state nmr studies of powders of amino acids and dipeptides and in reasonable agreement with quantum-chemical dft calculat ...	2004	15630560
microbiology of infected hemangiomas in children.	bacterial infections are a common complication of hemangiomas in children. the objective of this study was to establish the aerobic and anaerobic microbiology of infected hemangiomas. a retrospective 8-year review of clinical and microbiology laboratory records from patients with secondarily infected hemangiomas was carried out. specimens from infected sites were processed for the presence of aerobic and anaerobic bacteria. bacterial growth was present in 32 of 38 specimens. aerobic bacteria alo ...	2004	15078348
down-modulation of the antigen receptor by a superantigen for human b cells.	b cell superantigens (sags) have been implicated in human diseases by demonstrating non-clonotypic expansion of b cells bearing certain immunoglobulin variable region genes. one possibility is that, during infection with microorganisms secreting sags, these potent molecules might modulate bcr expression. to test this hypothesis, we investigated the potential effects of a sag, protein l from peptostreptococcus magnus, on antigen b cell receptor (bcr) surface expression in vitro. using fluorescenc ...	2004	15081532
radiology quiz case 2. cervical necrotizing fasciitis (cnf), odontogenic origin.		2004	15096437
septic arthritis after arthroscopic anterior cruciate ligament reconstruction.	a retrospective review of postoperative infected anterior cruciate ligament (acl) reconstruction was done on 472 consecutive cases in one institution. the purpose was to assess the incidence, diagnosis, treatment and outcome factors.	2004	15098639
the microbial synergy of peptostreptococcus micros and prevotella intermedia in a murine abscess model.	this study characterized the microbial interaction of peptostreptococcus micros and prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine model. subcutaneous injection of p. micros cells in the dorsum of the mouse together with living cells of p. intermedia resulted in a significantly larger abscess when compared with single injection of the organisms (p < 0.02). the abscess size was also significantly increased (p < 0.05) when the plate-cultured cell suspension o ...	2004	15107069
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2002 and march 2003].	tendency of isolated bacteria from infections in general surgery during the period from april 2002 to march 2003 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 334 strains were isolated from 131 (75.3%) of 174 patients with surgical infections. one hundred and seventy-one strains were isolated from primary infections, and 163 strains were isolated from post-operative infections. from primary infections, anaerobic gram-positive bacteria ...	2004	15116573
surveillance of susceptibility patterns in 1297 european and us anaerobic and capnophilic isolates to co-amoxiclav and five other antimicrobial agents.	in vitro susceptibility data were collected for co-amoxiclav and other antimicrobial agents against 1297 recent anaerobe isolates collected in europe and the usa. the co-amoxiclav (amoxicillin/clavulanic acid) mic(50/90)s (amoxicillin/clavulanic acid concentration in a ratio of 2:1, expressed in terms of amoxicillin concentration in mg/l) were 0.5/4 for bacteroides fragilis, </=0.125/1 for prevotella species, </=0.125/0.25 for fusobacterium nucleatum, 0.5/1 for eikenella corrodens, 0.25/8 for pe ...	2004	15128729
systematic 16s rrna gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans.	clinical microorganisms isolated during a 5-year study in our hospital that could not be identified by conventional criteria were studied by 16s rrna gene sequence analysis. each isolate yielded a > or =1,400-bp sequence containing <5 ambiguities which was compared with the genbank 16s rrna gene library; 1,404 such isolates were tested, and 120 were considered unique (27 isolates) or rare (< or =10 cases reported in the literature) human pathogens. eleven new species, "actinobaculum massiliae," ...	2004	15131188
antibiotic resistance profile of the subgingival microbiota following systemic or local tetracycline therapy.	tetracyclines have been extensively used as adjunctives to conventional periodontal therapy. emergence of resistant strains, however, has been reported. this study evaluated longitudinally the tetracycline resistance patterns of the subgingival microbiota of periodontitis subjects treated with systemic or local tetracycline therapy+scaling and root planing (srp).	2004	15142209
nitroimidazole resistance genes (nimb) in anaerobic gram-positive cocci (previously peptostreptococcus spp.).	to investigate metronidazole resistance and the prevalence of nitroimidazole (nim) genes in clinically isolated anaerobic gram-positive cocci.	2004	15150170
changes in oral microbial profiles after periodontal treatment as determined by molecular analysis of 16s rrna genes.	terminal rflp (t-rflp) analysis was used to investigate changes in the oral microbiota in saliva and subgingival plaque samples from one patient with aggressive periodontitis (subject a) and two patients with chronic periodontitis (subjects b and c) before and 3 months after periodontal treatment. substantial changes in the t-rflp patterns of subgingival plaque samples of subjects b and c were noted after 3 months of improved oral hygiene and full-mouth supra- and subgingival scaling and root pl ...	2004	15150339
apical periodontitis development and bacterial response to endodontic treatment. experimental root canal infections in monkeys with selected bacterial strains.	in six monkeys, 160 root canals were inoculated with a combination of four bacterial strains belonging to species streptococcus milleri, peptostreptococcus anaerobius, prevotella oralis, and fusobacterium nucleatum. in two other monkeys, 24 root canals were inoculated with a five-strain combination consisting of these strains and a strain of enterococcus faecalis. all strains were previously isolated from an infected monkey root canal. after 8-12 months, survival of the strains was recorded bact ...	2004	15154917
in vitro activities of the new semisynthetic glycopeptide telavancin (td-6424), vancomycin, daptomycin, linezolid, and four comparator agents against anaerobic gram-positive species and corynebacterium spp.	telavancin is a new semisynthetic glycopeptide anti-infective with multiple mechanisms of action, including inhibition of bacterial membrane phospholipid synthesis and inhibition of bacterial cell wall synthesis. we determined the in vitro activities of telavancin, vancomycin, daptomycin, linezolid, quinupristin-dalfopristin, imipenem, piperacillin-tazobactam, and ampicillin against 268 clinical isolates of anaerobic gram-positive organisms and 31 corynebacterium strains using agar dilution meth ...	2004	15155214
specific in vivo deletion of b-cell subpopulations expressing human immunoglobulins by the b-cell superantigen protein l.	some pathogens have evolved to produce proteins, called b-cell superantigens, that can interact with human immunoglobulin variable regions, independently of the combining site, and activate b lymphocytes that express the target immunoglobulins. however, the in vivo consequences of these interactions on human b-cell numbers and function are largely unknown. using transgenic mice expressing fully human immunoglobulins, we studied the consequences of in vivo exposure of protein l of peptostreptococ ...	2004	15155659
the bacteriology of the supragingival plaque of child dental patients in kuwait.	to investigate the bacterial flora of the gingival plaques of pediatric dental patients in order to establish baseline data for future studies on the complex microbiota of the oral cavity of this group of child dental patients.	2004	15181322
orbital cellulitis caused by peptostreptococcus.		2004	15184929
whole-body autoradiography reveals that the peptostreptococcus magnus immunoglobulin-binding domains of protein l preferentially target b lymphocytes in the spleen and lymph nodes in vivo.	protein l is an immunoglobulin (ig)-binding protein produced by the gram-positive bacterium peptostreptococcus magnus that interacts with the variable region of ig kappa light chains. the ig light chain-binding capacity of protein l gives it the potential to interact with cells expressing surface ig such as b cells. the present study was performed to address the in vivo trafficking of protein l at both the organ and the cellular level. using the powerful technique of whole-body autoradiography i ...	2004	15186398
subgingival microbiota of chilean patients with chronic periodontitis.	an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago.	2004	15212354
itih4 (inter-alpha-trypsin inhibitor heavy chain 4) is a new acute-phase protein isolated from cattle during experimental infection.	we have isolated from calf serum a protein with an apparent m(r) of 120,000. the protein was detected by using antibodies against major acute-phase protein in pigs with acute inflammation. the amino acid sequence of an internal fragment revealed that this protein is the bovine counterpart of itih4, the heavy chain 4 of the inter-alpha-trypsin inhibitor family. the response of this protein in the sera was determined for animals during experimental bacterial and viral infections. in the bacterial ...	2004	15213118
chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis.	the etiology of ulcerative colitis (uc) is unknown, but evidence links it to bacteria belonging to the normal colonic microbiota. the aims of this study were to characterize bacteria colonizing the rectal epithelium, and to investigate whether significant differences existed in uc. rectal biopsy specimens were obtained via endoscopy from 9 patients with active colitis and 10 patients without inflammatory bowel disease. complex bacterial communities colonized the rectal mucosa in all subjects. ov ...	2004	15227614
[a case of infected emphysematous bulla successfully treated with percutaneous thoracic drainage].	we present a case of infected emphysematous bulla successfully treated by percutaneous drainage. a 39-year-old man was admitted to our hospital because of fever and right lateral chest pain. a chest radiograph and ct revealed a cystic lesion with an air fluid level in the upper lobe of the right lung. the infected emphysematous bulla was not improved by the administration of antibiotics. on day 6 of hospitalization, a drainage tube (aspiration kit: 6 fr) was inserted percutaneously into the bull ...	2004	15228143
crystal structure and biological implications of a bacterial albumin binding module in complex with human serum albumin.	many bactericide species express surface proteins that interact with human serum albumin (hsa). protein pab from the anaerobic bacterium finegoldia magna (formerly peptostreptococcus magnus) represents one of these proteins. protein pab contains a domain of 53 amino acid residues known as the ga module. ga homologs are also found in protein g of group c and g streptococci. here we report the crystal structure of hsa in complex with the ga module of protein pab. the model of the complex was refin ...	2004	15269208
[bacterial infections in chronic otitis media--usefulness of molecular diagnostics based on pcr method].	infections in chronic otitis media (com) are common, and identification of their pathogen is difficult, what is proven by high rate of negative results of bacteriological examinations. this fact may be explained by two factors: it is difficult to obtain a reliable material for culture and classic methods of bacteriological cultures may be of low sensitivity. amplification of bacterial dna in pcr is an examination, that leads to a higher effectiveness and sensitivity of bacteriological tests. the ...	2004	15311593
initial antimicrobial effect of controlled-release doxycycline in subgingival sites.	to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp).	2004	15324352
beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis.	this study assessed the extent of beta-lactamase-producing bacteria in subgingival plaque samples obtained from 25 patients with refractory marginal periodontitis in the usa. beta-lactamase-positive isolates were characterized using commercial diagnostic kits and partial sequencing of the 16s rrna gene. the susceptibilities to different antimicrobial agents were tested and, in addition, the isolates were screened for the presence of extended spectrum beta-lactamases (esbls). beta-lactamase-produ ...	2004	15327642
microbiological profile of early onset/aggressive periodontitis patients.	the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence.	2004	15327644
dna-dna relatedness and phylogenetic positions of slackia exigua, slackia heliotrinireducens, eggerthella lenta, and other related bacteria.	recently, two asaccharolytic eubacterium species, eubacterium exiguum and eubacterium lentum, and peptostreptococcus heliotrinreducens have been reclassified as slackia exigua, eggerthella lenta and slackia heliotrinireducens in the novel genera on the basis of 16s rdna sequence analysis. but dna-dna relatedness among these species and other related bacteria have not been reported yet. dna-dna relatedness is the standard arbiter and the recommended method for the designation and evaluation of ne ...	2004	15327650
relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy.	severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions.	2004	15341348
intra-abdominal, retroperitoneal, and visceral abscesses in children.	this review describes the microbiology, diagnosis, and management of intra-abdominal abscesses (including subphrenic, hepatic, splenic, and retroperitoneal abscesses) in children. they often occur as a complication of local or generalized peritonitis, commonly secondary to appendicitis, necrotizing enterocolitis, pelvic inflammatory disease, and tubo-ovarian infection, surgery or trauma. the original infection generally occurs because of the entry of enteric microorganisms into the peritoneal ca ...	2004	15343468
[biological properties of microorganisms as the basis for the prognosis of the severity of pyoinflammatory pleuropulmonary diseases].	in the study of 65 microbial cultures isolated from the purulent foci in acute pulmonary abscess and acute pyothorax of 48 patients, a wide spectrum of microflora was detected. staphylococci and pseudomonas prevailed among aerobes, bacteroids and peptostreptococci--among anaerobes. in cases of the prolonged course of the pathological process, as compared with the common one, microorganisms exhibited hemolytic activity and high antilysozyme and anticomplementary levels. these findings served as t ...	2004	15346941
surgical infections: a microbiological study.	surgical infections are mostly polymicrobial, involving both aerobes and anaerobes. one hundred seventeen cases comprised of abscesses (n=51), secondary peritonitis (n=25), necrotizing fascitis (n=22) and wounds with devitalized tissues (n=19) were studied. the number of microorganisms isolated per lesion was highest in secondary peritonitis (2.32). the aerobe/ anaerobe ratio was 0.81 in secondary peritonitis and 1.8 in necrotizing fascitis. most secondary peritonitis (80%), necrotizing fascitis ...	2004	15361989
clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis.	the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy.	2004	15367191
small intestinal submucosa tension-free sling: postoperative inflammatory reactions and additional data.		2004	15371839
variation in antimicrobial action of proanthocyanidins from dorycnium rectum against rumen bacteria.	the proanthocyanidin polymer fractions of the leaves of the forage legume dorycnium rectum were analysed by acid catalysis with benzyl mercaptan, nmr and es-ms. the results showed that d. rectum differs from other temperate proanthocyanidin-containing forage legumes in that the range of polymers extends up to very high degrees of polymerisation. three fractions were characterised as low, medium, and high molecular weight proanthocyanidin fractions with mean degree of polymerisations of 10.3, 41 ...	2004	15381413
the role of sequence and structure in protein folding kinetics; the diffusion-collision model applied to proteins l and g.	the diffusion-collision model (dcm) is applied to the folding kinetics of protein l and protein g. in the dcm, the two proteins are treated as consisting of two beta-hairpins and one alpha-helix, so that they are isomorphous with the three-helix bundle dcm model. in the absence of sequence dependent factors, both proteins would fold in the same way in the dcm, with the coalescence of the n-terminal hairpin and the helix slightly favored over the c-terminal hairpin and the helix because the forme ...	2004	15458632
the effect of periodontal treatment on the salivary bacterial load and early plaque formation.	the purpose of the present study was to investigate in periodontitis patients the relationship between the number of bacteria in the saliva and the amount of de novo plaque formation before and after treatment.	2004	15491312
subgingival microbiota of chronic periodontitis subjects from different geographic locations.	most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries.	2004	15491316
retrospective case review of capsular contracture after two-stage breast reconstruction: is colonization of the tissue expander pocket associated with subsequent implant capsular contracture?	periprosthetic capsular contracture is a common problem associated with implant-based breast reconstruction. the purpose of this study was to determine if bacterial colonization of the tissue expander contributes to contracture of the permanent implant. medical records were reviewed for 86 patients (124 tissue expanders) between 1997 and 2001 in 1 institution. three specimens taken from the expander were cultured. the overall incidence of colonization was 42.7%; 49.4% (38.8-60.0) of immediate an ...	2004	15502455
rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis.	although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ...	2004	15508748
role of anaerobic bacteria in infections following tracheostomy, intubation, or the use of ventilatory tubes in children.	colonization of the tracheobronchial tree with microorganisms almost always follows tracheal intubation, tracheostomy, or the use of ventilatory tubes. infection of the tracheostomy wound site frequently occurs after prolonged use of the tracheostomy. the long-term-ventilated child is at high risk for developing tracheobronchitis or nosocomial pneumonia, generally involving aerobic gram-negative or gram-positive bacteria. several studies have illustrated the role of anaerobic bacteria in lower r ...	2004	15535147
pharmacokinetics and pharmacodynamics of intravenous levofloxacin at 750 milligrams and various doses of metronidazole in healthy adult subjects.	the purpose of this investigation was to evaluate the steady-state pharmacokinetics, pharmacodynamics, and safety of intravenous levofloxacin at 750 mg administered once daily combined with three different dosages of intravenous metronidazole (500 mg every 8 h [q8h], 1,000 mg q24h, and 1,500 mg q24h). eighteen healthy adult subjects received all three combinations in a randomized, crossover fashion. serial blood and urine samples were collected on the third day of each study period. the 24-h are ...	2004	15561831
microbiology and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses.	this review describes the microbiology, diagnosis, and management of peritonsillar, retropharyngeal, and parapharyngeal abscesses in children. predominant anaerobic organisms isolated in peritonsillar, lateral pharyngeal, and retropharyngeal abscesses are prevotella, porphyromonas, fusobacterium and peptostreptococcus spp.; aerobic organisms are group a streptococcus ( streptococcus pyogenes ), staphylococcus aureus and haemophilus influenzae . anaerobic bacteria can be isolated from most absces ...	2004	15573356
[a case of retroperitoneal abscess by anaerobic bacteria].	a case of retroperitoneal abscess with an unknown origin is presented. a 60-year-old woman visited our hospital complaining of fever and right flank pain. abdominal computed tomography and ultrasound examination revealed retroperitoneal abscess posterior to right kidney. percutaneous drainage and administration of antibiotics were performed. then, both symptoms were improved. the fluid culture proved to be anaerobic bacteria, peptostreptococcus species and porphyromonas asaccharolytica. she had ...	2004	15575225
microbiological basis of oral infections and sensitivity to antibiotics.	because oral infections are common, the physician must understand the underlying etiology, pathogeny, and other variables that determine how these processes evolve in order to choose the most appropriate antibiotic drug. the special characteristics of the oral cavity determine the make-up of the microflora that lives there. different anaerobic species belonging to the peptostreptococcus, prevotella, fusobacterium, gemella, and porphyromonas genera are of particular interest, as are the aerobic s ...	2004	15580129
periimplantitis.	orodental rehabilitation through the use of implants offers very high success rates. in this paper, we describe some of the complications involved with this technique, such as periimplant disease and, within this category, periimplantitis, an inflammatory reaction in which there is a loss of the bony support of the implant accompanied by inflammation. the aetiology of the disease is conditioned by the status of the tissue surrounding the implant, implant design, degree of roughness, the poor ali ...	2004	15580138
socket infection due to retained gauze after evisceration.	a 64-year-old man had late-onset socket infection due to retained surgical gauze after evisceration. external examination showed a mass of retained surgical gauze with copious yellowish discharge and gas bubbles. computed tomography scans showed a 3.2 x 2.4 x 2.4-cm heterogeneous mass and numerous gas bubbles. culture of the discharge yielded pseudomonas aeruginosa, streptococcus viridans, peptostreptococcus species, and fusobacterium species. surgical debridement and antibiotic therapy achieved ...	2004	15580975
clinical and microbiological associations in chronic periodontitis.	chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ...	2004	15584257
frozen cancellous bone allografts: positive cultures of implanted grafts in posterior fusions of the spine.	we have carried out a study on the behaviour pattern of implanted allografts initially stored in perfect conditions (aseptically processed, culture-negative and stored at -80 degrees c) but which presented positive cultures at the implantation stage. there is no information available on how to deal with this type of situation, so our aim was to set guidelines on the course of action which would be required in such a case. this was a retrospective study of 112 patients who underwent a spinal arth ...	2004	14648307
observation and characterization of the interaction between a single immunoglobulin binding domain of protein l and two equivalents of human kappa light chains.	detailed stopped-flow studies in combination with site-directed mutagenesis, isothermal titration calorimetry data and x-ray crystallographic knowledge have revealed that the biphasic pre-equilibrium fluorescence changes reported for a single ig-binding domain of protein l from peptostreptococcus magnus binding to kappa light chain are due to the binding of the kappa light chain at two separate sites on the protein l molecule. elimination of binding site 2 through the mutation a66w has allowed t ...	2004	14668335
anaerobic microbiology in 198 cases of pleural empyema: a bulgarian study.	the aim of the study was to evaluate the incidence of anaerobic bacteria in 198 patients with pleural empyema and the susceptibility of isolates to eight antibacterial agents. isolates were identified by the crystal anaerobes identification system, api system rapid id 32 a and/or routine methods. susceptibility was tested by sceptor mic system for anaerobic bacteria and limited agar dilution method. anaerobic bacteria were found in 74.2% of the patients and included 247 strains within 21 genera. ...	2004	16701526
[frequency, diagnostic criteria and consequences of bacterial vaginosis in pregnant women].	vaginal discharge based on changes in a vaginal ecosystem (decreasing number of lactobacillus and increasing number of mobiluncus spp., gardnerella vaginalis, prevotella spp., mycoplasma hominis, peptostreptococcus spp) appears to be a major cause of bacterial vaginosis. bacterial vaginosis is associated with: preterm delivery, premature rupture of membranes, chorioamnionitis, amniotic fluid infection, endometritis, low-weight neonates. the role of natural microflora of vagina, the most importan ...	2004	15730019
use of bioinformatics and pcr in the search for abc transporter homology among various bacteria.	bioinformatics databases and search tools are utilised to produce polymerase chain reaction (pcr) primers for the amplification of an abc transporter gene from the clinically important anaerobe finegoldia magna. on sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of enterococcus faecalis. little sequence data is available for f. magna and the newly isolated dna could be a useful tool in the identification of this organism in clinical specimens.	2004	15649009
alteration in the endogenous intestinal flora of swiss webster mice by experimental angiostrongylus costaricensis infection.	the association between worm infections and bacterial diseases has only recently been emphasized. this study examined the effect of experimental angiostrongylus costaricensis infection on endogenous intestinal flora of swiss webster mice. eight mice aging six weeks were selected for this experiment. four were infected with a. costaricensis and the other four were used as controls. twenty eight days after the worm infection, all mice in both groups were sacrificed and samples of the contents of t ...	2004	15654427
bacteriology of diabetic foot lesions.	clinical grading and bacteriological study of 107 patients with diabetic foot lesions revealed polymicrobial aetiology in 69 (64.4%) and single aetiology in 21 (19.6%). among 107 patients 62 had ulcer. of these 31 had mixed aerobes. twenty six patients with cellulitis and 12 with gangrene had more than 5 types of aerobes and anaerobes such as e. coli, klebsiella spp., pseudomonas spp., proteus spp., enterobactor spp., enterococci spp., clostridium perfringens, bacteroides spp., prevotella spp. a ...	2004	17642727
anaerobic liver abscesses as initial presentation of silent colonic cancer.	rarely, multiple liver abscesses caused by anaerobic organisms are the presenting form of unsuspected colonic cancer in the absence of liver metastases.	2004	18333045
the use of pcr for the identification and characterisation of bacteriocin genes from bacterial strains isolated from rumen or caecal contents of cattle and sheep.	pcr primers were designed to amplify the gene that encodes bovicin 255 from streptococcus gallolyticus lrc0255 and the bacteriocin genes from butyrivibrio fibrisolvens strains ar10 and or79a (bvid and bvi79a) in order to screen for their incidence in rumen and caecal b. fibrisolvens and streptococcus bovis-like isolates from new zealand and north american ruminants. none of the b. fibrisolvens-like strains (n=34) isolated from new zealand or north america had the genes encoding for butyrivibrioc ...	2004	19712403
synthesis and screening of a rationally designed combinatorial library of affinity ligands mimicking protein l from peptostreptococcus magnus.	rational design and combinatorial chemistry were utilized to search for lead protein l (ppl) mimetics for application as affinity ligands for the purification of antibodies and small fragments, such as fab and scfv, and as potential diagnostic or therapeutic agents. inspection of the key structural features of the complex between ppl and human fab prompted the de novo design and combinatorial synthesis of a 169-membered solid-phase ligand library, which was assessed for binding to human igg and ...	2005	15688433
periodontal infections and pre-term low birth weight: a case-control study.	pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ...	2005	15691348
traumatic cholesteatoma presenting as a brain abscess: a case report.	to discuss the clinical aspects and management of traumatic cholesteatomas of the temporal bone.	2005	15699721
recovery of anaerobic bacteria from wounds after lawn-mower injuries.	accidental injury while using lawn mowers can cause serious infectious complications in the injured extremity. anaerobic bacteria were rarely recovered from this infection. two children who sustained injury in their foot by a lawn mower developed severe wound infection. culture of the wound from 1 patient had heavy growth of clostridium bifermentans and peptostreptococcus magnus, and the culture from the other child grew clostridium perfringens. antimicrobial therapy directed at the pathogens an ...	2005	15699821
pharmacokinetics and pharmacodynamics of linezolid in obese patients with cellulitis.	linezolid is an oxazolidinone antimicrobial with excellent oral bioavailability and tissue penetration and is active against multidrug-resistant skin/soft tissue pathogens.	2005	15701775
influence of antimicrobial treatment for helicobacter pylori infection on the intestinal microflora in japanese macaques.	eradication treatment for helicobacter pylori is known to cause mild but relatively frequent adverse effects. some adverse effects such as diarrhoea and soft stools are related to disruption of the composition of the intestinal microflora. this study investigated the microfloral changes resulting from administration of an eradication regimen using proton pump inhibitor (ppi), amoxycillin and clarithromycin. twenty-eight laboratory-bred japanese macaques either were administered eradication treat ...	2005	15713617
micromonas (peptostreptococcus) micros: unusual case of prosthetic joint infection associated with dental procedures.	micromonas (peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract infections, but only rarely has been reported as a pathogenic agent of prosthetic joint infections. here we describe a case of a 63-year-old woman with prosthetic joint infection of total hip arthroplasty caused by the anaerobic species micromonas micros, associated with tooth extraction. samples obtained intraoperatively and from the or ...	2005	15715175
binding of actinobacillus actinomycetemcomitans lipopolysaccharides to peptostreptococcus micros stimulates tumor necrosis factor alpha production by macrophage-like cells.	peptostreptococcus micros is a gram-positive bacterium that has been associated with periodontitis and endodontic infections. in this study, we hypothesized that p. micros binds the immunomodulating component lipopolysaccharide derived from gram-negative bacteria to increase its capacity to stimulate cytokine production by host cells. the ability of p. micros to bind actinobacillus actinomycetemcomitans lipopolysaccharide was demonstrated by an enzyme-linked immunosorbent assay and by immunoelec ...	2005	15720573
properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host.	peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec 1.4.1.2) overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ...	2005	15727821
[bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004].	tendency of isolated bacteria from infections in general surgery during the period from april 2003 to march 2004 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 455 strains including 14 strains of candida spp. were isolated from 191(75.2%) of 254 patients with surgical infections. two hundred and thirty-nine strains were isolated from primary infections, and 216 strains were isolated from postoperative infections. from primary infection ...	2005	15997655
on the origin of the thermostabilization of proteins induced by sodium phosphate.	the b1 domain of protein l shows a linear rise in thermostability with increasing concentrations of sodium phosphate. equal behavior is observed for a set of mutant proteins where surface lysines are mutated to noncharged residues, but the mutant's thermostabilities show different sensitivities to phosphate, encoded in the varying slopes observed (mi). the melting temperature in the absence of the cosolute also correlates linearly with mi. the stabilizing effect of the phosphate ion reaches a sa ...	2005	15998065
antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain.	antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands.	2005	15998275
bacteriology of acute and chronic ethmoid sinusitis.	aspirates from 26 acutely and 17 chronically infected ethmoid sinuses were studied. thirty-seven aerobes and 10 anaerobes were recovered from isolates from patients with acute sinusitis. streptococcus pneumoniae and haemophilus influenzae were predominant. twenty-seven aerobes and 41 anaerobes were found in isolates from patients with chronic sinusitis. the predominant isolates were anaerobic gram-negative bacilli and peptostreptococcus spp.	2005	16000483
toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria.	the colonic epithelium provides an interface between the host and micro-organisms colonising the gastrointestinal tract. molecular recognition of bacteria is facilitated through toll-like receptors (tlr). the colonic epithelium expresses relatively high levels of mrna for tlr3 and less for tlr2 and -4. little is known of the expression patterns and mode of induction of expression for these pattern recognition receptors in human colon. the aim of this study was to investigate their localization i ...	2005	16011525
microbial analysis of the autogenous bone collected by bone filter during oral surgery: a clinical study.	the purposes of the study presented are to identify the microbial content of the collected bone debris and to determine the antibacterial efficiency of chlorhexidine mouth rinse in reducing the microbial content of the collected bone debris.	2005	16243175
[bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?].	the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity.	2005	16261393
effect of sodium alendronate on alveolar bone resorption in experimental periodontitis in rats.	bisphosphonates are potent inhibitors of bone resorption and were shown to inhibit bone resorption in experimental periodontitis by unknown mechanisms. we studied the effect of the aminobisphosphonate sodium alendronate (sa) in experimental periodontitis. wistar rats were subjected to ligature placement around the second upper left molars.	2005	16274309
[two cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae].	most of bartholin's gland abscesses have been thought to be caused by microorganisms found in opportunistic infections. however, we have encountered two very interesting cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae, two major pathogens of respiratory tract infections. in the first case, since abscess formation was not observed due to disintegration, cefdinir (cfdn), 300 mg/day, t.i.d. for 5 days was administered. the treatment improved clinic ...	2005	16276738
the effect of haart on salivary microbiota in the women's interagency hiv study (wihs).	study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study.	2005	16301151
microbiology of intracranial abscesses and their associated sinusitis.	to evaluate the organisms recovered from infected sinuses and associated intracranial abscesses (ias).	2005	16301376
bacterial etiology of diabetic foot infections in south india.	foot infections are a frequent complication of patients with diabetes mellitus, accounting for up to 20% of diabetes-related hospital admissions. infectious agents are associated with the worst outcomes, which may ultimately lead to amputation of the infected foot unless prompt treatment strategies are ensued. the present study sought to reveal the bacterial etiology of diabetic foot ulcers in south india, the diabetic capital of india.	2005	16314237
microbiology profile in women with pelvic inflammatory disease in relation to iud use.	to study the microbial characteristics of patients with pelvic inflammatory disease (pid) and the possible impact of an intrauterine device (iud) on the microbial environment in women presenting with pid.	2005	16338777
[bacterial 16s rdna sequence analysis of siberian tiger faecal flora].	bacterial 16s rdna library of siberian tiger was developed and 15 different clones were obtained using ecor i and hind iii in restriction fragment length polymorphism analysis. dna sequencing and similarity analysis showed that 10 clones matched corresponding clostridium sequences, of which 6 sequences had over 99% similarity with clostridium novyi type a, and 4 sequences had 97% similarity with swine manure bacterium rt-18b, which identified as peptostreptococcus spp. the other five 16s rdna se ...	2005	16342753
effect of mineral trioxide aggregate on cytokine production by peritoneal macrophages.	to test the effect of two commercial brands of grey mineral trioxide aggregate (proroot and mta-angelus) on cytokine production by m1 and m2 inflammatory macrophages.	2005	16343117
a case of cutaneous odontogenic sinus.	despite the fact that cutaneous sinus tracts of odontogenic origin are well documented, the condition is still commonly misdiagnosed, because chronic periapical periodontitis may be asymptomatic and is rarely open to the skin. a 75-year-old japanese woman presented to our clinic with the chief complaint of a left cheek skin lesion with mild pain. physical examination revealed a subcutaneous nodule covered with erythematous skin on her left buccal region. cultures from the subcutaneous nodule gre ...	2005	16361742
[severe pelvic inflammatory disease. response to ertapenem].	pelvic inflammatory disease (pid) is an important health problem. delay in diagnosis and effective treatment contributes to inflammatory sequelae with risk of future fertility problems and chronic pelvic pain. treatment regimens must provide empiric, broad-spectrum coverage of likely pathogens but also take into account patient's risk profile. in this article we report the case of a young woman affected by a rare genital malformation who required the creation of a neovagina made of abdominal ski ...	2005	16446794
infected pneumatocele following anaerobic pneumonia in adult.	we report a case of an infected pneumatocele in the course of anaerobic pneumonia in an adult. to the best of our knowledge, anaerobic pneumonia complicated by a pneumatocele in an adult has not previously been described. the pneumatocele occurred on the fifth day of hospitalization, and rapidly increased in size, with the development of a subsequent mixed anaerobe infection. a pig-tail catheter was inserted and the pus drained. the bacterial culture from the pus was positive for three anaerobes ...	2005	16491835
engineering high affinity superantigens by phage display.	protein l (ppl) is a b-cell superantigen from peptostreptococcus magnus known to bind to mammalian vkappa light chains. ppl from p.magnus strain 312 comprises five homologous immunoglobulin (ig) binding domains. we first analysed the binding of the individual domains (b1-b5) of ppl(312) to human vkappa light chains (huvkappa) subtypes 1 (huvkappai) and 3 (huvkappaiii). using a combination of rational design and phage selection we isolated mutants of the n-terminal b1 domain with a 14-fold increa ...	2005	15733921
microbiology and management of infected neck cysts.	to review and describe the microbiology, diagnosis, and management of infected neck cysts.	2005	15742293
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].	this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.	2005	15757581
anaerobic spondylodiscitis: case series and systematic review.	bacterial spondylodiscitis is rarely caused by anaerobic organisms. we describe two patients with lumbar vertebral osteomyelitis and discitis caused by anaerobic bacteria, including an unusual occurrence after an endodontic procedure, and review the salient clinical features and outcomes of 31 previously reported cases.	2005	15759942
influence of denatured and intermediate states of folding on protein aggregation.	we simulate the aggregation thermodynamics and kinetics of proteins l and g, each of which self-assembles to the same alpha/beta [corrected] topology through distinct folding mechanisms. we find that the aggregation kinetics of both proteins at an experimentally relevant concentration exhibit both fast and slow aggregation pathways, although a greater proportion of protein g aggregation events are slow relative to those of found for protein l. these kinetic differences are correlated with the am ...	2005	15772307
protein l-agarose for adsorption of autoantibodies: a potential tool for extracorporeal treatment.	this work investigated the adsorption of autoantibodies such as anti-ss-a/ro, anti-ss-b/la, anti-sm, and anti-dsdna on protein l-agarose gel. in order to determine better conditions for igg adsorption on this matrix, some buffer systems were tested. adsorption data were analyzed using the langmuir and langmuir-freundlich isotherm models. the experimental isotherms were best described by the langmuir-freundlich model, which indicated negative and positive cooperativities for binding in the presen ...	2005	15787626
detection of bacteria in platelet concentrates: comparison of broad-range real-time 16s rdna polymerase chain reaction and automated culturing.	based on real-time polymerase chain reaction (pcr) technology, a broad-range 16s rdna assay was validated and its performance was compared to that of an automated culture system to determine its usefulness for rapid routine screening of platelet concentrates (pcs).	2005	15847662
chicken anti-protein l for the detection of small amounts of protein l in the presence of igg.	protein l is a cell surface protein, expressed by peptostreptoccocus magnus, which binds to the variable light chains of immunoglobulins without interfering with antigen binding. it can be used for purification of mammalian antibodies of all classes in contrast to the ig-binding proteins protein a and protein g. detection of protein l leakage into antibody preparations is important, since protein l could interfere in immunological assays and cause adverse reactions in vivo. here we have develope ...	2005	15857176
mechanically unfolding the small, topologically simple protein l.	beta-sheet proteins are generally more able to resist mechanical deformation than alpha-helical proteins. experiments measuring the mechanical resistance of beta-sheet proteins extended by their termini led to the hypothesis that parallel, directly hydrogen-bonded terminal beta-strands provide the greatest mechanical strength. here we test this hypothesis by measuring the mechanical properties of protein l, a domain with a topology predicted to be mechanically strong, but with no known mechanica ...	2005	15863479
microbiology of acute and chronic maxillary sinusitis associated with an odontogenic origin.	: to study the microbiology of sinusitis associated with odontogenic origin.	2005	15867647
tobacco smoking and periodontal microflora in a saudi arabian population.	to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.	2005	15882210