Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [newly designed ultraviolet light monochromator]. | 2015 | 4542243 | |
| [current treatment of trichuriasis]. | 2015 | 4661971 | |
| [sudden death in alveolar proteinosis]. | 2015 | 4493287 | |
| the senile tremor patient. | 2015 | 4524036 | |
| hypersomatotropism and acanthosis nigricans in two brothers. | 2016 | 4843205 | |
| chromosomes and mental status. a study of women residing in institutions for the elderly. | 2016 | 4809925 | |
| parkinsonism, l-dopa, and intelligence. | 2016 | 4754685 | |
| the pragmatic utilization of the p-k phenomenon. | 2016 | 5039530 | |
| total body fat, calculated from body density, and its relationship to skinfold thickness in 571 people aged 12-72 years. | 2016 | 4760798 | |
| [examinations on the influence of arterial blood pressure on the course of glaucoma simplex]. | 2016 | 5095602 | |
| [clinico-histological and therapeutic considerations on histiocytosis x]. | 2016 | 4848308 | |
| isotope clearance studies in peripheral vascular reconstruction. | 2016 | 4862727 | |
| subacute sclerosing encephalitis. | 2016 | 4862504 | |
| letter: beyond cost estimating, another ball game. | 2016 | 4820302 | |
| the elbow in arthrogryposis. | 2016 | 4766189 | |
| [incision for reduction of tension in burned hands]. | 2016 | 5010206 | |
| [letter: influence of meteorological conditions on the occurrence of spontaneous pneumothorax]. | 2016 | 4849084 | |
| degradation products and the role of coagulation in "persistent" glomerulonephritis. | 2016 | 4931741 | |
| [anatomo-pathological, clinical and statistical considerations on the phenomenon of sudden death]. | 2016 | 4921485 | |
| [autoimmune hepatitis]. | 2016 | 4865241 | |
| [condensation of phenols with halogenoacids using boron trifluoride (author's transl)]. | 2016 | 4775944 | |
| separation of steryl acetates by silver ion chromatography. | 2016 | 5028412 | |
| the renal excretion and tubular reabsorption of citric acid in renal tubular acidosis. | 2016 | 5052389 | |
| [physical training at mental hospitals]. | 2016 | 4987623 | |
| no excuse for rh failure. | 2016 | 4981721 | |
| [premolarization of molars--its justification and clinical importance]. | 2016 | 4951125 | |
| thoracic duct lymph dialysis. | 2016 | 4882570 | |
| cycloheximide induced amnesia and recovery as a function of training parameters. | 2016 | 4857294 | |
| plasma lecithin-cholesterol acyltransferase deficiency in a child with terminal pulmonary hyaline membrane disease. | 2016 | 4800877 | |
| [mechanism of the lymphogenous propagation of breast cancer]. | 2016 | 4714889 | |
| [la fontaine and the apothecaries]. | 2016 | 4929537 | |
| [racemization during peptide synthesis]. | 2016 | 4881545 | |
| [clinical examination of patients with multiple injuries]. | 2016 | 5078491 | |
| joint hypermobility--asset or liability? a study of joint mobility in ballet dancers. | 2016 | 5016858 | |
| [variability of the oils of juniperus communis. 3. the oils in plants of different populations and growth-types (author's transl)]. | 2016 | 4859185 | |
| [letter: consent to donate organs]. | 2016 | 4849067 | |
| origins of time. a reconstruction of the ontogenetic development of the sense of time based on object-relations theory. | 2016 | 4832046 | |
| [improvement in hospital care]. | 2016 | 4700975 | |
| [antiparkinsonism drug (parkan) as a hallucinogen]. | 2016 | 4698717 | |
| effects of environmental variation on arousal during vigilance performance. | 2016 | 4711977 | |
| indirect myocardial revascularization. | 2016 | 5098978 | |
| [organization of sanatorial care for children with rheumatic fever]. | 2016 | 5081437 | |
| is jump to population-explosion bandwagon justified? | 2016 | 5009981 | |
| [early postoperative rehabilitation after maxillary resection]. | 2016 | 4951118 | |
| [circumstantial study of accidents in minors under 15 years of age (study of 100 cases)]. | 2016 | 4791562 | |
| non-surgical treatment of deep wounds triggered by harmful physical and chemical agents: a successful combined use of collagenase and hyaluronic acid. | some chronic ulcers often occur with slough, not progressing through the normal stages of wound healing. treatment is long and other therapies need to be performed in addition to surgery. patients not eligible for surgery because of asa class (american society of anesthesiologists class) appear to benefit from chemical therapy with collagenase or hydrocolloids in order to prepare the wound bed, promoting the healing process. we describe four cases of traumatic, upper limb deep wounds caused by d ... | 2016 | 24698215 |
| the grim-19 plays a vital role in shrimps' responses to vibrio alginolyticus. | grim-19 (gene associated with retinoid-interferon-induced mortality 19), a novel cell death regulatory gene, plays important roles in cell apoptosis, mitochondrial respiratory chain and immune response. it has been reported to interact physically with stat3 and inhibit stat3-dependent signal transduction. in this study, a new grim-19 gene, which is a 789-bp gene encoding a 149 amino acids protein, is identified and characterized from litopenaeus vannamei. the tissue distribution patterns showed ... | 2016 | 26702559 |
| molecular variations in vibrio alginolyticus and v. harveyi in shrimp-farming systems upon stress. | a study was performed to investigate the genomic variations in the shrimp farm isolates of vibrio alginolyticus and v. harveyi when the isolates were subjected to environmental stress. samples of shrimps, water and sediment were collected from southern indian coastal shrimp farms. vibrio isolates were biochemically identified and confirmed using 16s rdna and gyrb gene specific pcr. the bacterial strains were genotyped by pcr fingerprinting using gtg(5) and is (insertion sequence) primers. seven ... | 2016 | 26691457 |
| characterization of vibrio parahaemolyticus and its specific phage from shrimp pond in palk strait, south east coast of india. | phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. the aim of this study is to isolate and characterize the vibrio parahaemolyticus and its potential lytic phage from penaeus monodon growing-out by rearing in shrimp ponds in palk strait, south east coast of india. the conventional phenotypic characteristics and molecular identification was confirmed using 16s rrna sequence and to determine the antibiotic re ... | 2016 | 27697363 |
| complete genome sequence of vibrio alginolyticus zj-t. | vibrio alginolyticus is a ubiquitous gram-negative bacterium which is normally distributed in the coastal and estuarine environments. it has been suggested to be an opportunistic pathogen to both marine animals and humans, here, the completed genome sequence of v. alginolyticus zj-t was determined by illumina high-throughput sequencing. | 2016 | 27587824 |
| studies on the mechanism of bacterial flagellar rotation and the flagellar number regulation. | many motile bacteria have the motility organ, the flagellum. it rotates by the rotary motor driven by the ion-motive force and is embedded in the cell surface at the base of each flagellar filament. many researchers have been studying its rotary mechanism for years, but most of the energy conversion processes have been remained in mystery. we focused on the flagellar stator, which works at the core process of energy conversion, and found that the periplasmic region of the stator changes its conf ... | 2016 | 27581279 |
| characterisation and expression analysis of two terminal complement components: c7 and c9 from large yellow croaker, larimichthys crocea. | the large yellow croaker larimichthys crocea, as one of the most economically important marine fish in china and east asian countries, are facing the fatal attraction of various pathogens in recent years. elucidation of the organism immunomodulatory mechanism of croaker response to pathogen infection is essential for the disease control. in present study, we reported for the first time the molecular characterization and expression analysis of two terminal complement components (tccs) of croaker, ... | 2016 | 26902705 |
| abundant members of scavenger receptors family and their identification, characterization and expression against vibrio alginolyticus infection in juvenile larimichthys crocea. | scavenger receptors (srs) are crucial pattern recognition receptors (prrs) to defense pathogen infection in fish innate immunity. in this paper, some members in srs family of larimichthys crocea were identified, including eight genes in the class a, b, d and f families. (g + c) % of all srs members held 51% ∼ 59%, and these genes were no obvious codon bias by analyzing the distribution of a-, t-, g- and c-ended codons. the order of enc for all srs members by sequencing was lyccd68 > lycscara5 > ... | 2016 | 26876357 |
| the tca pathway is an important player in the regulatory network governing vibrio alginolyticus adhesion under adversity. | adhesion is a critical step in the initial stage of vibrio alginolyticus infection; therefore, it is important to understand the underlying mechanisms governing the adhesion of v. alginolyticus and determine if environmental factors have any effect. a greater understanding of this process may assist in developing preventive measures for reducing infection. in our previous research, we presented the first rna-seq data from v. alginolyticus cultured under stress conditions that resulted in reduced ... | 2016 | 26870007 |
| the known two types of transglutaminases regulate immune and stress responses in white shrimp, litopenaeus vannamei. | transglutaminases (tgs) play critical roles in blood coagulation, immune responses, and other biochemical functions, which undergo post-translational remodeling such as acetylation, phosphorylation and fatty acylation. two types of tg have been identified in white shrimp, litopenaeus vannamei, and further investigation on their potential function was conducted by gene silencing in the present study. total haemocyte count (thc), differential haemocyte count (dhc), phenoloxidase activity, respirat ... | 2016 | 26855013 |
| molecular characterization and function of a pten gene from litopenaeus vannamei after vibrio alginolyticus challenge. | pten, a tumor suppressor gene, suppresses cell survival, growth, apoptosis, cell migration and dna damage repair by inhibiting the pi3k/akt signaling pathway. in this study, the full-length litopenaeus vannamei pten (lvpten) cdna was obtained, containing a 5'utr of 59bp, an orf of 1269bp and a 3'utr of 146bp besides the poly (a) tail. the pten gene encoded a protein of 422 amino acids with an estimated molecular mass of 48.3 kda and a predicted isoelectric point (pi) of 7.6. subcellular localiza ... | 2016 | 26801100 |
| dynamics of snake-like swarming behavior of vibrio alginolyticus. | swarming represents a special case of bacterial behavior where motile bacteria migrate rapidly and collectively on surfaces. swarming and swimming motility of bacteria has been studied well for rigid, self-propelled rods. in this study we report a strain of vibrio alginolyticus, a species that exhibits similar collective motility but a fundamentally different cell morphology with highly flexible snake-like swarming cells. investigating swarming dynamics requires high-resolution imaging of single ... | 2016 | 26910435 |
| molecular cloning, characterization and expression analysis of (b-cell lymphoma-2 associated x protein) bax in the orange-spotted grouper (epinephelus coioides) after the vibrio alginolyticus challenge. | bax is a pro-apoptotic member of bcl-2 like superfamily, playing an important role in regulating the apoptosis. in this study, the full-length bax (ecbax) was obtained, containing a 5'utr of 64 bp, an orf of 579 bp and a 3'utr of 1021 bp. the ecbax gene encoded a polypeptide of 192 amino acids with an estimated molecular mass of 21.55 kda and a predicted isoelectric point (pi) of 6.75. the deduced amino acid sequence analysis showed that ecbax comprised the conserved residues and the characteris ... | 2016 | 26905633 |
| isolation and characterization of two lytic bacteriophages, φst2 and φgrn1; phage therapy application for biological control of vibrio alginolyticus in aquaculture live feeds. | bacterial infections are a serious problem in aquaculture since they can result in massive mortalities in farmed fish and invertebrates. vibriosis is one of the most common diseases in marine aquaculture hatcheries and its causative agents are bacteria of the genus vibrio mostly entering larval rearing water through live feeds, such as artemia and rotifers. the pathogenic vibrio alginolyticus strain v1, isolated during a vibriosis outbreak in cultured seabream, sparus aurata, was used as host to ... | 2016 | 26950336 |
| identification of dld, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three vibrio species in epinephelus coioides. | vibrio spp. represent a serious threat to the culture of epinephelus coioides (orange-spotted grouper) in southeast asia. in this study we used two-dimensional electrophoresis (2-de) and western blotting to identify common immunogenic proteins of vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus. membranes were probed with orange-spotted grouper anti-v. alginolyticus sera and accordingly 60, 58 and 48 immunogenic protein spots were detected. by matching analysis for the three west ... | 2016 | 26562319 |
| identification and characterization of a mitochondrial unfolded protein response transcription factor atfs-1 in litopenaeus vannamei. | a mitochondrial specific stress response termed mitochondrial unfolded protein response (upr(mt)) is activated in responding to disturbance of protein homeostasis in mitochondria. the activating transcription factor associated with stress-1 (designated as atfs-1) is the key regulator of upr(mt). to investigating the roles of atfs-1 (lvatfs-1) in litopenaeus vannamei mitochondrial stress remission and immunity, it's full length cdna was cloned. the open reading frame of lvatfs-1 was 1, 557 bp in ... | 2016 | 26481519 |
| effects of methanolic macroalgae extracts from caulerpa sertularioides and ulva lactuca on litopenaeus vannamei survival in the presence of vibrio bacteria. | macroalgae are potentially excellent sources of highly bioactive secondary metabolites that are useful for the development of new functional ingredients. this study was conducted to determine whether methanolic extracts from caulerpa sertularioides and ulva lactuca macroalgae might be possible alternatives for the prevention of shrimp vibriosis, which is caused by vibrio parahaemolyticus and vibrio alginolyticus. macroalgae extracts prepared with methanol as the solvent were evaluated for antiba ... | 2016 | 26915309 |
| differential expression of micrornas in shrimp marsupenaeus japonicus in response to vibrio alginolyticus infection. | till date numerous micrornas (mirnas) have been discovered from various organisms, including mammals, plants, insects, nematodes and viruses. they are known to have antiviral functions in crustaceans such as shrimp marsupenaeus japonicas. however, little is known about the role of mirnas against bacterial infection in this shrimp caused by vibrio alginolyticus. we performed small rna sequencing to characterize the differentially expressed micrornas in v. alginolyticus challenged shrimp, in compa ... | 2016 | 26483347 |
| antimicrobial susceptibility of vibrio alginolyticus isolated from oyster in korea. | pathogenic vibrio alginolyticus, a cause of severe infection in shellfish, as well as in humans, has been found at high frequency around all coastal areas of korea. the aim of this study was to determine the occurrence of v. alginolyticus, to identify the strains isolated from oysters in west sea, and to investigate their antimicrobial resistance profiles. biochemical analyses of the 90 initially recovered presumptive v. alginolyticus colonies indicated that 16 isolates were v. alginolyticus. pc ... | 2016 | 27543129 |
| cloning and characterization of tyrosine hydroxylase (th) from the pacific white leg shrimp litopenaeus vannamei, and its expression following pathogen challenge and hypothermal stress. | tyrosine hydroxylase (th) belongs to the biopterin-dependent aromatic amino acid hydroxylase enzyme family, and it represents the first and rate-limiting step in the synthesis of catecholamines that are required for physiological and immune process in invertebrates and vertebrates. cloned litopenaeus vannamei th (lvth), containing a short alpha helix domain, a catalytic core, a regulatory domain, a phosphorylation site and two potential n-linked glycosylation sites as presented in vertebrate and ... | 2016 | 27514780 |
| beta-1,3-1,6-glucan modulate the non-specific immune response to enhance the survival in the vibrio alginolyticus infection of taiwan abalone (haliotis diversicolor supertexta). | this research aims to investigate the non-specific immune response of taiwan abalone (haliotis diversicolor supertexta) which was treated with the beta-1,3-1,6-glucan to be observed in the survival impact after the vibrio alginolyticus infection. the non-specific immune and physiological response of superoxide anion radical (o2(-)), phenoloxidase (po), phagocytic index (pi), phagocytic rate (pr) and lucigenin-chemiluminescence for reactive oxygen intermediates (rois) were enhanced via in-vitro e ... | 2016 | 27150049 |
| transcriptome analysis of the pearl oyster (pinctada fucata) hemocytes in response to vibrio alginolyticus infection. | the pearl oyster pinctada fucata is cultured widely for production of marine pearls in china, while mass mortalities, likely related to pathogenic infections, have occurred frequently in juvenile, mother and operated oysters. to address this issue, understanding host defense mechanisms of p. fucata against pathogenic challenge is extremely important. in the present study, a comparative analysis of hemocyte transcriptomes of p. fucata before and after vibrio alginolyticus infection was conducted ... | 2016 | 26363408 |
| comparative genomic analysis of six new-found integrative conjugative elements (ices) in vibrio alginolyticus. | vibrio alginolyticus is ubiquitous in marine and estuarine environments. in 2012-2013, sxt/r391-like integrative conjugative elements (ices) in environmental v. alginolyticus strains were discovered and found to occur in 8.9 % of 192 v. alginolyticus strains, which suggests that v. alginolyticus may be a natural pool possessing resourceful ices. however, complete ice sequences originating from this bacterium have not been reported, which represents a significant barrier to characterizing the ice ... | 2016 | 27145747 |
| complete genome sequence of a giant vibrio phage valkk3 infecting vibrio alginolyticus. | this paper describes the complete sequence of a giant lytic marine myophage, vibrio phage valkk3 that is specific to vibrio alginolyticus atcc(®) 17749™. vibrio phage valkk3 was subjected to whole genome sequencing on miseq sequencing platform and annotated using blast2go. the complete sequence of valkk3 genome was deposited in dbbj/embl/genbank under accession number kp671755. | 2016 | 27114905 |
| discovery, structural characterization and functional analysis of alpha-2-macroglobulin, a novel immune-related molecule from holothuria atra. | the non-specific protease inhibitor alpha-2-macroglobulin (a2m) is a key macromolecular glycoprotein that involved in host immune defense against pathogens in vertebrates and invertebrates. however, no research regarding a2m has been developed in echinoderms to date. in this study, the full-length cdna of a2m was cloned from the sea cucumber (holothuria atra), which is a tropical species widely distributed along the coasts of the south china sea and designated haa2m. haa2m possesses all three co ... | 2016 | 27033585 |
| the bacterial (vibrio alginolyticus) production of tetrodotoxin in the ribbon worm lineus longissimus-just a false positive? | we test previous claims that the bacteria vibrio alginolyticus produces tetrodotoxin (ttx) when living in symbiosis with the nemertean lineus longissimus by a setup with bacteria cultivation for ttx production. toxicity experiments on the shore crab, carcinus maenas, demonstrated the presence of a paralytic toxin, but evidence from lc-ms and electrophysiological measurements of voltage-gated sodium channel-dependent nerve conductance in male wistar rat tissue showed conclusively that this effect ... | 2016 | 27023570 |
| serine suppresses the motor function of a periplasmic pomb mutation in the vibrio flagella stator. | the flagellar motor of vibrio alginolyticus is made of two parts: a stator consisting of proteins poma and pomb, and a rotor whose main component is flig. the interaction between flig and poma generates torque for flagellar rotation. based on cross-linking experiments of double-cys mutants of pomb, we previously proposed that a conformational change in the periplasmic region of pomb caused stator activation. double-cys mutants lost their motility due to an intramolecular disulfide bridge. in thi ... | 2016 | 27004994 |
| transcriptome analysis of the effect of vibrio alginolyticus infection on the innate immunity-related tlr5-mediated induction of cytokines in epinephelus lanceolatus. | epinephelus lanceolatus, considered to be an aquaculture fish species of high economic value in east asia, is one of the largest groupers in the epinephelus genus. vibrio alginolyticus is a bacterial species that causes high morbidity in marine fish; infection can cause exophthalmia, ulcers, septicemia, and corneal opaqueness in fish. epinephelus lanceolatus larvae infected with vibrio alginolyticus were subjected to transcriptome analysis to study the immune regulation pathway. grouper larvae w ... | 2016 | 26975410 |
| the immune responses and antioxidant status of portunus trituberculatus individuals with different body weights. | vibrio alginolyticus is a virulent pathogen that affects crab aquacultures. in the present study, the immune responses and antioxidant status of big and small (based on body weight and size) 80-, 100- and 120-day-old specimens of portunus trituberculatus, challenged for 72 h with vibrio alginolyticus, were studied. the total hemocyte count (thc), and phagocytic, prophenoloxidase and phenoloxidase activities, of the big individuals (bis) were higher than those of the small individuals (sis) (p < ... | 2016 | 26952172 |
| potentiometric aptasensing of vibrio alginolyticus based on dna nanostructure-modified magnetic beads. | a potentiometric aptasensing assay that couples the dna nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of vibrio alginolyticus is herein described. the dna nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. the solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chrono ... | 2016 | 27918423 |
| filtering activity on a pure culture of vibrio alginolyticus by the solitary ascidian styela plicata and the colonial ascidian polyandrocarpa zorritensis: a potential service to improve microbiological seawater quality economically. | we investigated and compared, by laboratory experiments, the filter-feeding activity on bacteria by the solitary ascidian styela plicata and the colonial ascidian polyandrocarpa zorritensis. clearance rates and retention efficiencies were estimated by using, as only food source, the bacterial species vibrio alginolyticus selected on account of its importance in aquaculture pathogenicity. the cmax was 1.4±0.17lh(-1)g(-1) dw for s. plicata and 1.745lh(-1)g(-1) dw for p. zorritensis. the highest re ... | 2016 | 27552728 |
| chromatin immunoprecipitation sequencing technology reveals global regulatory roles of low-cell-density quorum-sensing regulator apha in the pathogen vibrio alginolyticus. | quorum sensing (qs) is an important regulatory system in virulence expression and environmental adaptation in bacteria. the master qs regulators (mqsr) luxr and apha reciprocally control qs gene expression in vibrios. however, the molecular basis for the regulatory functions of apha remains undefined. in this study, we characterized its regulatory roles in vibrio alginolyticus, an important zoonotic pathogen causing diseases in marine animals as well as in humans. apha is involved in the motilit ... | 2016 | 27551022 |
| a novel biomarker for marine environmental pollution of hsp90 from mytilus coruscus. | heat shock protein 90 (hsp90) is a conserved molecular chaperone contributing to cell cycle control, organism development and the proper regulation of cytosolic proteins. the full-length hsp90 cdna of mytilus coruscus (mchsp90, kt946644) was 2420bp, including an orf of 2169bp encoding a polypeptide of 722 amino acids with predicted pi/mw 4.89/83.22kda. blastp analysis and phylogenetic relationship strongly suggested mchsp90 was a member of hsp90 family, and it was highly conserved with other kno ... | 2016 | 27491367 |
| collagenase from vibrio alginolyticus cultures: experimental study and clinical perspectives. | purpose the purpose of this study is to examine the activity of collagenase from cultures of vibrio alginolyticus as in vitro as in biological samples and to evaluate clinical perspectives of this product about the treatment of fibroproliferative diseases like dupuytren's contracture. methods the experimental part of the study has been divided in 2 stages. in the first stage, the collagenase has been produced in laboratory, assessing its purity, verifying the in vitro degradation of collagen by ... | 2016 | 27448558 |
| spirulina elicits the activation of innate immunity and increases resistance against vibrio alginolyticus in shrimp. | the effect of spirulina dried powder (sdp) on the immune response of white shrimp litopenaeus vannamei was studied in vitro and in vivo. incubating shrimp haemocytes in 0.5 mg ml(-1) sdp caused the degranulation of haemocytes and a reduction in the percentage of large cells within 30 min. shrimp haemocytes incubated in 1 mg ml(-1) sdp significantly increased their phenoloxidase (po) activity, serine proteinase activity, and respiratory burst activity (rb, release of superoxide anion). a recombin ... | 2016 | 27368541 |
| an alpha-2 macroglobulin in the pearl oyster pinctada fucata: characterization and function in hemocyte phagocytosis of vibrio alginolyticus. | alpha-2 macroglobulin (α2m) is a ubiquitous protease inhibitor and considered to be an evolutionarily conserved constituent of innate host defence system. here, an α2m gene (designated as pfα2m) was obtained from the pearl oyster pinctada fucata by rt-pcr, pcr walking and rapid amplification of cdna ends (race). the pfα2m cdna consists of 6394 bp with an open reading frame (orf) of 5745 bp encoding a protein of 1914 amino acids with a 19 residues signal peptide. pfα2m sequence contains three put ... | 2016 | 27346151 |
| molecular cloning and characterization of pten in the orange-spotted grouper (epinephelus coioides). | pten is a key tumor suppressor gene that can play a regulatory role in the cellular proliferation, survival and apoptosis. in this study, the full-length pten (ecpten) was obtained, containing a 5'utr of 745 bp, an orf of 1269 bp and a 3'utr of 106 bp. the ecpten gene encoded a polypeptide of 422 amino acids with an estimated molecular mass of 49.14 kda and a predicted isoelectric point (pi) of 6.34. the deduced amino acid sequence analysis showed that ecpten comprised the conserved residues and ... | 2016 | 27725262 |
| characterization of potential lytic bacteriophage against vibrio alginolyticus and its therapeutic implications on biofilm dispersal. | vibrio alginolyticus is a leading cause of vibriosis, presenting opportunistic infections to humans associated with raw seafood contamination. at present, phage therapy that acts as an alternative sanitizing agent is explored for targeting v. alginolyticus. the study outcome revealed that the phage vp01 with its extreme lytic effect showed a high potential impact on the growth of v. alginolyticus as well as biofilm formation. electron microscopy revealed the phage resemblance to myoviridae, base ... | 2016 | 27793690 |
| hubp, a polar landmark protein, regulates flagellar number by assisting in the proper polar localization of flhg in vibrio alginolyticus. | the marine bacterium vibrio alginolyticus has a single polar flagellum, the number of which is regulated positively by flhf and negatively by flhg. flhf is intrinsically localized at the cell pole, whereas flhg is localized there through putative interactions with the polar landmark protein hubp. here we focused on the role of hubp in the regulation of flagellar number in v. alginolyticus deletion of hubp increased the flagellar number and completely disrupted the polar localization of flhg. it ... | 2016 | 27573015 |
| a σe-mediated temperature gauge controls a switch from luxr-mediated virulence gene expression to thermal stress adaptation in vibrio alginolyticus. | in vibrios, the expression of virulence factors is often controlled by luxr, the master quorum-sensing regulator. here, we investigate the interplay between luxr and σe, an alternative sigma factor, during the control of virulence-related gene expression and adaptations to temperature elevations in the zoonotic pathogen vibrio alginolyticus. an rpoe null v. alginolyticus mutant was unable to adapt to various stresses and was survival-deficient in fish. in wild type v. alginolyticus, the expressi ... | 2016 | 27253371 |
| complete genome sequence of vibrio alginolyticus atcc 33787(t) isolated from seawater with three native megaplasmids. | vibrio alginolyticus, an opportunistic pathogen, is commonly associated with vibriosis in fish and shellfish and can also cause superficial and ear infections in humans. v. alginolyticus atcc 33787(t) was originally isolated from seawater and has been used as one of the type strains for exploring the virulence factors of marine bacteria and for developing vaccine against vibriosis. here we sequenced and assembled the whole genome of this strain, and identified three megaplasmids and three type v ... | 2016 | 27211073 |
| [post nearly drowning vibrio alginolyticus septicemia acquired in reunion (indian ocean)]. | abstractwe report the first case of vibrio alginolyticus septicemia in the indian ocean (reunion island), in a patient (70-year-old-man) with multiple underlying conditions, following a nearly drowning in the lagoon of reunion. from now on, v. alginolyticus should be considered as a possible agent of septicemia in the indian ocean, particularly following marine activities. | 2016 | 27325174 |
| identification of nod like receptor c3 (nlrc3) in asian seabass, lates calcarifer: characterisation, ontogeny and expression analysis after experimental infection and ligand stimulation. | nod like receptors (nlrs) are a large group of cytoplasmic prrs believed to play an important role in bacterial recognition in higher vertebrates. in this study, a novel nod like receptor c3 (asnlrc3) has been identified, cloned and characterised from asian seabass, lates calcarifer. the full-length asnlrc3 transcript composed of a 4142 bp nucleic acid sequence encode for a protein of 1134 deduced amino acids. three signature domains identified are conserved nacht-domain, c-terminal llr domain a ... | 2016 | 27346158 |
| molecular detection of the three major pathogenic vibrio species from seafood products and sediments in tunisia using real-time pcr. | vibrio spp. have emerged as a serious threat to human health worldwide. v. parahaemolyticus , v. cholerae , and v. vulnificus pose a considerable public health risk in tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. more recently, toxr-positive v. alginolyticus was also reported to be a potential source of contaminated seafood. a total of 247 samples, including 113 fishes ( labrus viridis , penaeus ... | 2016 | 28221954 |
| dietary administration of saponin stimulates growth of the swimming crab portunus trituberculatus and enhances its resistance against vibrio alginolyticus infection. | the immunostimulatory role of dietary saponins on swimming crabs was investigated under field conditions. portunus trituberculatus were fed diets enriched with quillaja saponin (qs) at 150, 300 and 450 mg kg(-1). they had an enhanced growth rate and increased resistance against vibrio alginolyticus compared to crabs not fed with qs. significant effects were observed on the average body weight, percentage weight gain and specific growth rate (p < 0.05). total hemocyte and hyaline cell counts of p ... | 2016 | 27815204 |
| identification, characterization and expression analysis of tumor suppressor protein p53 from pufferfish (takifugu obscurus) after the vibrio alginolyticus challenge. | the tumor suppressor protein p53 plays a critical role in cell cycle, apoptosis and dna repair. in this study, the full-length pufferfish p53 (pf-p53) was obtained, containing an open reading frame of 1095 bp, a 5'utr of 157 bp and a 3'utr of 285 bp with a poly (a) tail. the pf-p53 encoded a polypeptide of 364 amino acids with a theoretical isoelectric point of 8.03 and predicted molecular weight of 40.6 kda. pf-p53 was ubiquitously expressed in various tissues with a high-level expression in ki ... | 2016 | 27815196 |
| mutational analysis and overproduction effects of motx, an essential component for motor function of na+-driven polar flagella of vibrio. | the bacterial flagellar motor is a rotary motor complex composed of various proteins. the motor contains a central rod, multiple ring-like structures and stators. the na(+)-driven polar flagellar motor of the marine bacterium vibrio alginolyticus has a specific ring, called the 't-ring', which consists of two periplasmic proteins, motx and moty. the t-ring is essential for assembly of the torque-generating unit, the poma/pomb stator complex, into the motor. to investigate the role of the t-ring ... | 2016 | 27803076 |
| identification of odontella aurita by rbcl gene sequence - a high antibacterial potential centric marine diatom. | the antibacterial potential of centric marine diatoms has been compared against the clinical pathogens and identified the potential diatom by rbcl gene sequencing. totally, five diatoms namely odontella aurita, thalassiosira subtilis, chaetoceros curvisetus, skeletonema costatum and coscinodiscus centralis were isolated from cuddalore coastal waters. the diatoms were morphologically identified and isolated using micro capillary-pipette and serial dilution method. the isolated diatoms were cultur ... | 2016 | 27159722 |
| complicated sea urchin-induced wound infection caused by vibrioalginolyticus and staphylococcus lugdunensis in a 14-year-old boy. | wound infections with vibrio alginolyticus, a gram-negative bacterium found in all temperate oceans, are rarely reported. however, a rising incidence of wound infections caused by v. alginolyticus requires better knowledge about this infectious agent. | 2016 | 28348795 |
| white shrimp litopenaeus vannamei that have received fucoidan exhibit a defense against vibrio alginolyticus and wssv despite their recovery of immune parameters to background levels. | white shrimp litopenaeus vannamei receiving fucoidan at 2, 6, and 10 μg g(-1) after 0-144 h or 0-120 h were examined for immune parameters (haemograms, phenoloxidase activity, respiratory burst, and superoxide dismutase activity), proliferation of haemocyte in the haematopoietic tissue (hpt), gene expression, and phagocytic activity and clearance efficiency to vibrio alginolyticus. immune parameters and mitotic index of hpt increased after 3-24 h, reached their maxima after 48-72 h, and returned ... | 2016 | 27815206 |
| a novel p38 mapk indentified from crassostrea hongkongensis and its involvement in host response to immune challenges. | p38 mitogen-activated protein kinases (mapks) are conserved serine/threonine-specific kinases that are activated by various extracellular stimuli and play crucial regulatory roles in immunity, development and homeostasis. however, the function of p38s in mollusks, the second most diverse group of animals, is still poorly understood. in this study, a novel molluscan p38 (designated chp38) was cloned and characterized from the hong kong oyster crassostrea hongkongensis. its full-length cdna encode ... | 2016 | 27768933 |
| isolation and characterization of agar-digesting vibrio species from the rotten thallus of gracilariopsis heteroclada zhang et xia. | gracilariopsis heteroclada zhang et xia (gracilariaceae, rhodophyta) is one of the most studied marine seaweeds due to its economic importance. this has been cultivated extensively on commercial scale in the philippines and other asian countries. however, sustainable production of g. heteroclada in the philippines could not be maximized due to the occurrence of rotten thallus disease. thus, isolation and characterization of agar-digesting bacteria from the rotten thalli of g. heteroclada was con ... | 2016 | 27285614 |
| genome-wide detection of predicted non-coding rnas related to the adhesion process in vibrio alginolyticus using high-throughput sequencing. | the ability of bacteria to adhere to fish mucus can be affected by environmental conditions and is considered to be a key virulence factor of vibrio alginolyticus. however, the molecular mechanism underlying this ability remains unclear. our previous study showed that stress conditions such as exposure to cu, pb, hg, and low ph are capable of reducing the adhesion ability of v. alginolyticus. non-coding rnas (ncrnas) play a crucial role in the intricate regulation of bacterial gene expression, t ... | 2016 | 27199948 |
| saharan dust nutrients promote vibrio bloom formation in marine surface waters. | vibrio is a ubiquitous genus of marine bacteria, typically comprising a small fraction of the total microbial community in surface waters, but capable of becoming a dominant taxon in response to poorly characterized factors. iron (fe), often restricted by limited bioavailability and low external supply, is an essential micronutrient that can limit vibrio growth. vibrio species have robust metabolic capabilities and an array of fe-acquisition mechanisms, and are able to respond rapidly to nutrien ... | 2016 | 27162369 |
| whole-genome sequence of vibrio alginolyticus isolated from the mucus of the coral fungia danai in the andaman sea, india. | vibrio alginolyticus, a halophilic gram-negative bacterium, which is found in temperate marine and estuarine environments, is known to cause infections in humans and other organisms. we sequenced the genome of sulfamethoxazole-trimthoprim-positive v. alginolyticus strain 4-19 isolated from the mucus of the coral fungia danai in the andaman sea, india. | 2016 | 27151803 |