Publications

pathways for the penetration of enteroinvasive yersinia into mammalian cells.	for many bacterial species, entry into mammalian cells is an important step toward establishing an infectious disease. genetic and molecular techniques have revealed many important features of the entry process. as an example of this approach, the enteric pathogen yersinia pseudotuberculosis has been used as a model system for bacterial penetration. this analysis has uncovered at least three different pathways for entry of the microorganism into cultured mammalian cells. these pathways differ in ...	1990	2182969
reactive arthritis following yersinia pseudotuberculosis infection.		1990	2190403
the cytotoxic protein yope of yersinia obstructs the primary host defence.	it has previously been shown that the plasmid-encoded yope protein of yersinia pseudotuberculosis is a virulence determinant. in this study, hela cells, macrophages and mice were used as different model systems to determine the actual role of yope in the virulence process. the yope protein mediates a cytotoxic response on a confluent layer of hela cells. a prerequisite of this activity is that the pathogen binds to the cell surface. yope also induces a cytotoxic response on mouse macrophages whe ...	1990	2191183
genetic evidence that the yopa gene-encoded yersinia outer membrane protein yop1 mediates inhibition of the anti-invasive effect of interferon.	hep-2 cell monolayers were challenged with genetic variants of yersinia pseudotuberculosis ypiii(pib1) and yersinia enterocolitica w22708(pyl4). both strains were represented by (i) variants harboring the 70-kilobase virulence plasmid, (ii) their isogenic plasmid-cured derivatives, and (iii) two transposon mutants constructed by insertional inactivation of the plasmid genes encoding outer membrane protein yop1 and ca2+ dependency in strains ypiii(pib1) and w22708(pyl4). when the hep-2 cells were ...	1990	2194966
[monoclonal antibodies: immunobiotechnologic principles of production and prospects of use].		1990	2197169
identification of a seventh operon on plasmid rk2 regulated by the kora gene product.	broad-host-range incp plasmids possess a series of operons involved in plasmid maintenance, whose expression is coordinated by a series of regulators, most of which are encoded in a central regulatory operon. the nucleotide sequence of a new monocistronic operon located between coordinates 55.0 and 56.0 kb on the genome of the incp alpha plasmids rk2 and rp4 is presented. the operon encodes a 34 kda protein which has a net negative charge. transcription of the operon, designated by us kfra (korf ...	1990	2197180
[the basic features of the pathogenesis of pseudotuberculosis (yersiniosis)].	a new pathogenetic pattern of pseudotuberculosis has been derived on the basis of original clinical, anatomical and experimental findings as well as literature data. careful consideration is given to: a basic mode of infection (alimentary), emergence of the primary affection with a rapid blood invasion by the agent (primary bacteremia), development of specific sensitization, multiorgan secondary focal impairment with lymphogenic dissemination of the infective agent (specific pseudotuberculous po ...	1990	2204325
[clinico-allergic indicators in children with yersinia infection].		1990	2204873
yersiniosis.		1990	2208969
the role of chemosensitive muscle receptors in cardiorespiratory regulation during exercise.	several possible mechanisms leading to the cardiorespiratory adjustments to muscular exercise can be considered. activation of the cardiovascular and respiratory centers may result from: (1) direct or reflex action of circulating metabolites (humoral control); (2) cortical influxes (central drive); (3) nervous impulses from receptors in the contracting muscles (peripheral drive). information presently available focuses most of the interest upon the muscular drive. our studies on anesthetized ani ...	1990	2212481
effect of magnesium lithospermate b on urinary prostaglandins in rats with renal failure.	urinary excretion of prostaglandin e2 showed a significant increase following administration of magnesium lithospermate b [1] in rats with renal failure. in contrast, that of thromboxane b2 showed a significant decrease, indicating the improvement of renal failure.	1990	2213036
homologous dna sequences on the virulence plasmids of pathogenic yersinia and salmonella dublin lane.	yersinia and salmonella harbour plasmids that encode traits important for virulence, enabling both pathogenic genera to survive and grow in cells of the reticulo-endothelial organs during systemic infections. we have detected dna homology between the salmonella dublin virulence plasmid psdl2 and the plasmids of the pathogenic yersinia species pestis, pseudotuberculosis, and enterocolitica. three regions of psdl2 were found to share homology with the virulence plasmid pib1 of yersinia pseudotuber ...	1990	2215215
[transduction of chromosome and plasmid markers of bacteriophage p1 in pseudotuberculosis pathogen].	a clone of bacteriophage p1 clr100 cml has been isolated capable of the general transduction in the cells of pseudotuberculosis causative agent. the genetic transfer of the 6 md pesticinogenicity plasmid by the bacteriophage has been used as a model to demonstrate the possibility of transduction. the bacteriophage used has been shown to be efficient in interspecies transduction between yersinia.	1990	2215519
yersiniosis in farmed deer.	samples from 77 chital (axis axis), 42 fallow (dama dama), 26 red (cervus elaphus), 7 rusa (cervus timorensis) and 1 sambar deer (cervus unicolor) were examined. yersinia pseudotuberculosis infection was diagnosed as the cause of death in 6 (23%) of the red and 23 (30%) of the chital deer. yersiniosis was the most common infectious cause of death diagnosed. affected deer were usually found moribund or dead, often with faecal staining of the perineum. gross pathology in chital included a fibrinou ...	1990	2222364
modification of the 31p magnetic resonance spectra of a rat tumour using vasodilators and its relationship to hypotension.	the effects of different doses of hydralazine and prostacyclin on the 31p magnetic resonance spectra of the lbds1 fibrosarcoma were investigated and related to their effects on mean arterial blood pressure (mabp) and heart rate. the effect of reducing mabp by bleeding the animals, via the tail artery, was also investigated. tumour spectral changes following high dose drug treatment (an increase in inorganic phosphate, a reduction in nucleotide triphosphates and a reduction in ph) were consistent ...	1990	2223572
modification of the 31p magnetic resonance spectra of a rat tumour using vasodilators and its relationship to hypotension.	the effects of different doses of hydralazine and prostacyclin on the 31p magnetic resonance spectra of the lbds1 fibrosarcoma were investigated and related to their effects on mean arterial blood pressure (mabp) and heart rate. the effect of reducing mabp by bleeding the animals, via the tail artery, was also investigated. tumour spectral changes following high dose drug treatment (an increase in inorganic phosphate, a reduction in nucleotide triphosphates and a reduction in ph) were consistent ...	1990	2223572
actinobacillus rossii sp. nov., actinobacillus seminis sp. nov., nom. rev., pasteurella bettii sp. nov., pasteurella lymphangitidis sp. nov., pasteurella mairi sp. nov., and pasteurella trehalosi sp. nov.	evidence from numerical taxonomic analysis and dna-dna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. the following new species are proposed: actinobacillus rossii sp. nov., from the vaginas of postparturient sows; actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; pasteurella bettii sp. nov., associated with human bartholin gland abscess and finger infections; pasteurella lymphangitidis sp. nov. (the blg group), w ...	1990	2223608
[plasmid dna transformation of yersinia pseudotuberculosis by electroporation].	rendering cells permeable to dna molecules, electroporation involves the application of high intensity electric fields of short duration to reversibly change the biomembranes, which has been used successfully in some species of both gram negative and positive bacteria. we first reported the electro-transformation technics of yersinia pseudotuberculosis. the plasmid pjgx20 was constructed by cloning the 9.5 kb plasmid encoding pesticin into pst i site of pbr328. transformation efficiency of 103 t ...	1990	2225008
sequence, localization and function of the invasin protein of yersinia enterocolitica.	the inv locus of yersinia enterocolitica is sufficient to convert a non-invasive escherichia coli k12 strain into a microorganism that is able to penetrate cultured mammalian cells. the nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin protein from yersinia pseudotuberculosis. a polyclonal antiserum elicited by a synthetic peptide corresponding to the c-terminal 88 amino acids of this open reading frame detected ...	1990	2233250
glycerol-blanked triglyceride assays with laboratory animals.		1990	2242600
[acute renal failure in a generalized form of pseudotuberculosis in children].		1990	2251062
morphine attenuation of a conditioned emotional response in post-dependent rats.	rats placed in a test chamber where they had received repetitive shocks the previous day significantly reduced their motor activity; this was taken as indicator of a conditioned emotional response. morphine attenuated this conditioned suppression of motility, possibly due to a reduction of the anxiety associated with the expectation of the noxious stimuli. previous morphine dependence (20 mg/kg daily for 26 days) did not modify the effect of the opioid on the conditioned suppression of motility. ...	1990	2253691
mice and moles inhabiting mountainous areas of shimane peninsula as sources of infection with yersinia pseudotuberculosis.	a total of 1,835 yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern shimane prefecture, japan. the yersinia spp. included 1,106 yersinia enterocolitica, 26 y. enterocolitica-like, 176 yersinia mollaretii, 149 yersinia frederiksenii, 70 yersinia intermedia, 231 yersinia kristensenii, 5 yersinia aldovae, and 72 yersinia pseudotuberculosis. human pathogenic y. enterocolitica was not isolated. y. pseudotuberculosis ...	1990	2254420
[from the parenchyma to the mucosa biopsy: significance and importance of local assays of antibiotics. a critical study].	the authors discussed the relevance of antibiotic concentrations as measured in tissues and fluids of the respiratory tract. they analysed theoretical factors which influence tissue distribution of antibiotics as well as the results of studies carried out in animal and in human tissues. various factors may interfere with the results of antibiotic assays: but the data published by various groups were usually convergent: this confirms the significance of "tissue pharmacokinetics". new protocols ba ...	1990	2270342
[clinical characteristics of pseudotuberculosis].		1990	2274827
[two cases of yersinia pseudotuberculosis infection in children].	yersinia pseudotuberculosis (y. pseudotuberculosis) infection is an intestinal infectious disease comparable in importance as those with campylobacter or salmonella. clinical symptoms of y. pseudotuberculosis infection vary. in this report, we will describe the clinical symptoms and immunological conditions of the patients with y. pseudotuberculosis infection, including 2 or our own cases. case 1 was a 4 years old male infant admitted to the hospital with major complaints of fever, diarrhea, and ...	1990	2286788
the pyv plasmid of yersinia encodes a lipoprotein, ylpa, related to trat.	a series of lipoproteins was detected in the membrane fraction of yersinia enterocolitica w227, a typical strain from serotype o:9. at least two of them, ylpa and ylpb, are encoded by the pyv plasmid. the sequence of ylpa reveals the presence of a typical lipoprotein signal peptide. the mature ylpa protein would be 223 residues long with a calculated molecular weight of 23798 for the proteic moiety of the molecule. ylpa shares 88% identical residues with the trat protein encoded by plasmid ped20 ...	1990	2287280
[glucocorticoid receptors in physiological states and in extreme conditions].	this review discusses glucocorticoid hormone--receptor interaction in the animal and human organism in the normal, extreme (stress, radiation, head-down tilt, etc.) and pathological situations. particular attention is given to advanced lines of research into glucocorticoid receptors within the framework of space biology and medicine. it is stressed that the data about glucocorticoid receptors may form the key that can help better understanding of changes induced by exposure to the space environm ...	1990	2292872
a model of multivessel coronary artery disease using conscious, chronically instrumented dogs.	the present investigation was undertaken to develop a reproducible model of multivessel coronary artery disease including a total occlusion of one vessel and a stenosis of an adjacent vessel in chronically instrumented dogs. utilizing sterile techniques, we surgically prepared dogs for measurement of heart rate, arterial pressure, left ventricular pressure, and blood flow velocity in the left anterior descending (lad) and left circumflex (lcca) coronary arteries. a hydraulic occluder and ameroid ...	1990	2292881
transdermal delivery of bioactive peptides: the effect of n-decylmethyl sulfoxide, ph, and inhibitors on enkephalin metabolism and transport.	we investigated the effects of the nonionic surfactant, n-decylmethyl sulfoxide (ndms), ph, and inhibitors on the metabolism and the permeation of amino acids, dipeptides, and the pentapeptide enkephalin, through hairless mouse skin. an hplc gradient method was developed to identify the possible peptide and amino acid metabolites of leucine-enkephalin. ndms increased the permeability of all amino acids and peptides tested. at neural ph, the enzyme activity within the skin was such that no flux o ...	1990	2293206
electron microscopic evidence for in vivo extracellular localization of yersinia pseudotuberculosis harboring the pyv plasmid.	electron microscopic evidence is presented that bacteria harboring the virulence plasmid pyv from yersinia pseudotuberculosis are localized in extracellular sites during the course of infection in mice, often unambiguously undergoing active replication. virulent pyv+ bacteria, often seen adherent to platelets, severely restricted granuloma formation, creating necrotic microabscesses poorly populated with inflammatory cells. this contrasts with granulomas produced by pyv- bacteria, which appear t ...	1990	2307522
genetic analysis of the yope region of yersinia spp.: identification of a novel conserved locus, yera, regulating yope expression.	the yope gene of yersinia pseudotuberculosis was recently sequenced, and yope was identified as an indispensable virulence determinant when tested in a mouse model (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). in the study described here, the dna sequences of the yope genes of yersinia pestis ev76 and yersinia enterocolitica 8081 were determined and compared with that of the y. pseudotuberculosis gene. only two codons were found to differ, both leading to amino acid replacemen ...	1990	2307658
multiple beta 1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells.	mammalian cell receptors that promote entry of intracellular bacteria into nonphagocytic cells have not been identified. we show here that multiple members of the integrin superfamily of cell adhesion receptors bind the y. pseudotuberculosis invasin protein prior to bacterial penetration into mammalian cells. affinity chromatography of crude detergent extracts demonstrated that integrins containing the subunit structures alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1 bound to immobilized inv ...	1990	2311122
synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32p-postlabeling analysis of human placental dna known to contain benzo[a]pyrene diol epoxide adducts.	human placenta readily catalyzes the biotransformation of polycyclic aromatic hydrocarbons (pahs) and other carcinogens to reactive metabolites that can damage dna through formation of covalent adducts. placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures in humans. however, previous studies of human placental dna have yielded discrepant results with respect to pah-dna adducts. in order to resolve some of the issues surrounding these discr ...	1990	2322996
rat toxicity studies with beta-sitosterol.	chronic administration of beta-sitosterol subcutaneously to rats for 60 days was well tolerated and there was no clear cut evidence of any gross or microscopic lesions either in the liver or kidney. liver and kidney function tests were assessed by determining the blood/serum parameters like haemoglobin, blood glucose, serum protein, serum bilirubin, serum cholesterol, serum gpt and serum got. all the parameters were in the normal range except serum protein and serum cholesterol. serum cholestero ...	1990	2329812
tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig.	anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ...	1990	2329918
a highly efficient electroporation system for transformation of yersinia.	the various pathogenic yersinia species are not readily and efficiently transformed by classical methods. for this reason, the electroporation technique was applied for genetic transformation of these species. using optimal conditions, we were able to transform the six yersinia strains studied with the two most widely used groups of plasmids: psu2718 (a pacyc184 derivative) and pk19 (a puc19 derivative). only yersinia enterocolitica (y. e.) serotype 0:8 gave poor results (less than 5 x 10(2) tra ...	1990	2332165
[plasmids of yersinia pseudotuberculosis].	plasmids with the sizes of 5.7; 51; 70-77; and 120-130 kb were found in six strains among the ten strains collection of yersinia pseudotuberculosis. the restriction endonucleases analysis. southern-blot hybridization and physical maps construction were performed for the plasmids. the 70-77 kb plasmids were found to be analogous to the ca2(+)-dependence plasmid pyvo19 from yersinia pestis ev76. the difference between the plasmids of this type is in the insertions or deletions located on the simil ...	1990	2333080
[conjugative r-plasmid resistance of the causative agents of yersinia infection].	nature, structure, occurrence and drug resistance of 160 strains of y. pseudotuberculosis and 60 strains of y. enterocolitica isolated from various sources within 1986-1988 were studied. in the strains of y. pseudotuberculosis, the cell composition with respect to the requirements in calcium ions as well as the plasmid profiles with determination of the molecular weights of the plasmids in the antibiotic sensitive and resistant pathogens and r(+)-transconjugants were investigated. some molecular ...	1990	2337369
[hepato-renal polycystic disease and liver hydatidosis (a rare association)].	a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ...	1990	2346686
relationship between dam's age at bull's birth and bull's genetic evaluation.	after accounting for year of birth of bulls, younger dams produced superior sons. bulls' pd, parent average, and pedigree index milk decreased with increasing age of dams at bull's birth. this appears to be due to decrease in maternal grandsire's pd milk and dam's cow index milk with increase in age of dam. younger dams have an advantage of potentially higher genetic merit due to genetic trend but have less information included in their evaluations. bull's latest parent average and pedigree inde ...	1990	2365889
[incidence of tuberculosis in the hunting tribe e-lun-chun in northeast china].	the e-lun-chun, or ortochen, a national minority in north east china, consisted of 2,262 members in 1953. in the mid-17th century a russian invasion had displaced them from their original home in southward direction over the amur river towards the sin an lin mountain range. while coming into closer contact with neighbouring peoples, tuberculosis and other infectious diseases spread rapidly among the members of the tribe. under the japanese occupation (1932-1945) the incidence of pulmonary tuberc ...	1990	2367435
ciprofloxacin penetration into human breast milk: a case report.	the concentration of ciprofloxacin achieved in human breast milk following oral administration of a single 500-mg dose was investigated. a 24-year-old white woman, 17 days postpartum, was being treated for resolving acute renal failure. oral ciprofloxacin was prescribed for a suspected urinary tract infection. due to suspected pediatric toxicity and a lack of published information describing ciprofloxacin excretion in breast milk, the antibiotic was discontinued after one 500-mg dose. all breast ...	1990	2375140
tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners.	the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ...	1990	2394748
effects of castration and testosterone treatment on the activity of testosterone-metabolizing enzymes in the brain of male and female zebra finches.	recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (taeniopygia guttata) brain using a sensitive radioenzyme assay combined to the palkovits punch method. a number of sex-differences in the activity of these enzymes were observed especially in nuclei of the song-control system. the hormonal controls of these differences have now been analyzed by gonadectomizing birds of both sexes and by giving them a ...	1990	2394994
[activity of antibacterial preparations against the causative agent of pseudotuberculosis in vitro].	antibacterial activity of 32 chemotherapeutics against 15 strains of yersinia ++pseudotuberculosis was studied in vitro. the majority of the strains or 80% were sensitive to penicillins, cephalosporins, monobactams, aminoglycosides, tetracyclins , anzamycins, fluorine derivatives of quinolonecarboxylic acid, levomycetin and a combination of trimethoprim and ++sulfamethoxazole. it was shown that with respect to the y. pseudotuberculosis strains with multiple antibiotic resistance, the fluorine de ...	1990	2400288
a ribosomal protein of yersinia pseudotuberculosis having partial epitope identity with hla-b27.	the phenotype hla-b27 is common in patients who develop reactive arthritis after having an infection. one hypothesis concerning the pathogenesis of reactive arthritis is that molecular mimicry between hla-b27 and certain bacterial components might be involved. it is known that an infection with yersinia is commonly associated with reactive arthritis in b27 positive patients. therefore, we were interested to investigate whether cross-reactivity between yersinia and hla-b27 exists. a gene library ...	1990	1715196
identification of the integrin binding domain of the yersinia pseudotuberculosis invasin protein.	the invasin protein of the pathogenic yersinia pseudotuberculosis mediates entry of the bacterium into cultured mammalian cells by binding several beta 1 chain integrins. in this study, we identified the region of invasin responsible for cell recognition. thirty-two monoclonal antibodies directed against invasin were isolated, and of those, six blocked cell attachment to invasin. these six antibodies recognized epitopes within the last 192 amino acids of invasin. deletion mutants of invasin and ...	1990	1693333
the yersinia enterocolitica inv gene product is an outer membrane protein that shares epitopes with yersinia pseudotuberculosis invasin.	an essential virulence attribute for yersinia enterocolitica and yersinia pseudotuberculosis is the ability to invade the intestinal epithelium of mammals. the chromosomal invasin gene (inv) has been cloned from both of these yersinia species, and the y. pseudotuberculosis invasin has been well characterized (r. r. isberg, d. l. voorhis, and s. falkow, cell 50:769-778, 1987). here we constructed tnphoa translational fusions to the y. enterocolitica inv gene to identify, characterize, and localiz ...	1990	1694524
thrombospondin deposition in rat carotid artery injury.	the balloon catheter injury model was used to determine the relative contributions of vascular smooth muscle cells (smc) and platelets to thrombospondin (tsp) antigen deposition in the artery wall. rat carotid arteries were denuded of endothelium, exposing the thrombogenic subendothelial extracellular matrix (ecm) to the circulation. rats were killed after 1 hour, or 5, 10, or 20 days. thrombospondin antigen deposition in the injured arteries was assessed using a specific polyclonal antiserum ra ...	1990	1695483
effects of substance p on [ca2+]i and force in intact guinea pig ileal smooth muscle.	in intact sheets of the guinea pig ileal longitudinal muscle, loaded with fura-2, both substance p (sp)- and k(+)-induced contractile responses are preceded by cytoplasmic free ca2+ ([ca2+]i) variations. in response to sp (10(-10)-10(-7) m), [ca2+]i and force increased concentration dependently. from 10(-9) m on, the response was biphasic: an initial ca2+ spike and force transient were followed by a tonic component. the [ca2+]i and force vs. log [sp] curves were sigmoidal for the initial phasic ...	1990	1695485
dna recombination and natural selection pressure sustain genetic sequence diversity of the feline mhc class i genes.	sequence comparisons of seven distinct mhc class i cdna clones revealed that feline class i molecules have a remarkable similarity to human hla genes in their organization of functional domains as well as in the nonrandom partitioning of genetic variability according to the functional constraints ascribed to different regions of the mhc molecule. the distribution of the pattern of sequence polymorphism in the cat as compared with genetic diversity of human and mouse class i genes provides eviden ...	1990	1695669
the postsynaptic 43k protein clusters muscle nicotinic acetylcholine receptors in xenopus oocytes.	nicotinic acetylcholine receptors (achrs) are localized at high concentrations in the postsynaptic membrane of the neuromuscular junction. a peripheral membrane protein of mr 43,000 (43k protein) is closely associated with achrs and has been proposed to anchor receptors at postsynaptic sites. we have used the xenopus oocyte expression system to test the idea that the 43k protein clusters achrs. mouse muscle achrs expressed in oocytes after injection of rna encoding receptor subunits are uniforml ...	1990	1698395
the postsynaptic 43k protein clusters muscle nicotinic acetylcholine receptors in xenopus oocytes.	nicotinic acetylcholine receptors (achrs) are localized at high concentrations in the postsynaptic membrane of the neuromuscular junction. a peripheral membrane protein of mr 43,000 (43k protein) is closely associated with achrs and has been proposed to anchor receptors at postsynaptic sites. we have used the xenopus oocyte expression system to test the idea that the 43k protein clusters achrs. mouse muscle achrs expressed in oocytes after injection of rna encoding receptor subunits are uniforml ...	1990	1698395
projection subtypes of rat neostriatal matrix cells revealed by intracellular injection of biocytin.	individual neostriatal-matrix spiny neurons were stained intracellularly with biocytin after intracellular recording in vivo, and their axons were traced into the globus pallidus (gp), entopeduncular nucleus (ep), and/or substantia nigra (sn). the locations of the neurons within the matrix compartment of the neostriatum (ns) were established by immunocytochemical counterstaining of sections containing the cell bodies using antibodies for calbindin-d28k. this allowed nearly complete visualization ...	1990	1698947
[quantitative determination of the carbohydrate determinants of o-antigens of yersinia pseudotuberculosis and pseudomonas fluorescens].	interactions of o-specific polysaccharides, lps and lps-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and monovalent fab-fragments of the antibodies were investigated. the association constants and quantity of fab-fragments bound to these antigens were established. the interaction of the lps and lps-protein complexes was shown to depend considerably on concentration of antigens, and only 10-15% of carbohydrate determinants are accessible fo ...	1990	1704703
[the biological action of yersinia pseudotuberculosis endotoxin].	experimental studies on guinea pigs have shown that y. pseudotuberculosis lipopolysaccharide is capable of inducing endotoxinemia accompanied by the development of the thrombohemorrhagic syndrome. in cases of pseudotuberculosis the importance of the increased synthesis of prostaglandins and cyclic nucleotides with the prevalence of pgf2 alpha and cgmp in the genesis of toxico-allergic manifestations of pathologic processes has been established. the pathomorphological picture of pseudotuberculosi ...	1990	1963723
[the protective properties of a porin from the outer membrane of yersinia pseudotuberculosis].	the protective properties of the species-specific pore-forming polypeptide of y. pseudotuberculosis outer membrane (porin) were studied. the study showed that 80-90% of mice immunized with porin survived after challenge with 10-30 ld50 of y. pseudotuberculosis, serovars 1 and 3. the ld50 of the preparation exceeded its ed50 more than 100-fold. immunization made in two injections was more effective than immunization in one injection. the immunization of the animals with porin led to an increase i ...	1990	1965874
[reactive arthritis following yersinia pseudotuberculosis infection].	a patient developed reactive arthritis due to yersinia pseudotuberculosis. five months after the primary infection, the patient still suffered from severe oligoarthritis. the administration of steroids and sulphasalazine had very little effect upon the development of the disease.	1990	1968686
a cloned chromosomal dna fragment which differentiates yersinia pestis from yersinia pseudotuberculosis and yersinia enterocolitica.	chromosomal dna from reference yersinia strains was digested individually with 9 restriction endonucleases. dna fragments were separated and analyzed by electrophoresis through agarose gels. the clearest fragment patterns were obtained when ecori was employed. the y. pestis fragment pattern obtained after the use of this enzyme showed the presence of a unique dna fragment with molecular mass 1400 bp. this dna fragment was cloned, purified, labeled with 32p and then used to probe ecori digests of ...	1990	1980253
application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in yersinia-triggered reactive arthritis.	leukocytes in synovial fluid and peripheral blood samples from patients with yersinia-triggered reactive arthritis were analyzed after dna amplification using the polymerase chain reaction. the primers applied were specific for the virulence plasmid-coded 1cre genes of yersinia enterocolitica o:3 and yersinia pseudotuberculosis iii. no yersinia dna was observed within the synovial fluid cells or peripheral blood cells by polymerase chain reaction techniques. however, yersinia antigens were detec ...	1991	1984781
combined use of released proteins and lipopolysaccharide in enzyme-linked immunosorbent assay for serologic screening of yersinia infections.	an elisa for the screening of serum antibodies to yersinia species was developed using plasmid-encoded released proteins of yersinia enterocolitica o:8 and lipopolysaccharide of y. enterocolitica o:3 as a combined antigen. of 43 sera from patients infected with one of six different yersinia serotypes, 40 (93%) were positive in this assay. when tested using six serotype-specific elisas with the corresponding yersinia bacteria as antigens, 38 (88%) were positive. this screening elisa detects antib ...	1991	1988526
[yersinia pseudotuberculosis in new world monkeys].	it is reported on four cases of pseudotuberculosis in 2 saimiri sciureus, 1 callithrix jacchus/penicillata hybrid and 1 s. oedipus. the animals came from two different groups, one being infected with y. pseudotuberculosis serotype i, the other with y. pseudotuberculosis serotype ii. all cases showed intestinal infection of thermophile campylobacter. remarkable were severe haemorrhagic components and the distinct rhs-proliferation, especially in the mesenteric lymphatic nodes.	1991	2015031
role of the yersinia outer membrane protein yada in adhesion to rabbit intestinal tissue and rabbit intestinal brush border membrane vesicles.	the yersinia virulence plasmid confers on strains of yersinia pseudotuberculosis and y. enterocolitica an adhesive potential superior to the one encoded by the chromosome alone. we have evaluated the role of the plasmid-encoded outer membrane protein yada (formerly called yopl) in adhesion. insertional inactivation of the yada gene (formerly called yopa), which encodes yada, led to a reduction in the capacity of plasmid-carrying strains of y. pseudotuberculosis 0:iii and y. enterocolitica 0:9 to ...	1991	2018636
control of pseudotuberculosis in zoos.		1991	2021043
risk of yersinia infection among butchers.	the purpose of this study was to evaluate the risk of yersinia infection among butchers. serum samples were collected from 146 abattoir workers, stratified into 3 groups according to exposure to swine throats and intestines. 100 healthy blood donors were used as controls. antibodies against yersinia enterocolitica o:3 and o:9 and y. pseudotuberculosis i and iii were measured using elisa. symptoms associated with yersiniosis were recorded in a questionnaire. antibodies against y. enterocolitica o ...	1991	2028228
pseudotuberculosis at the zoological society of london (1981 to 1987)		1991	2028568
acute tubulointerstitial nephritis associated with yersinia pseudotuberculosis infection.	two siblings infected with yersinia pseudotuberculosis suffered from acute renal failure about 2 weeks after the onset of the disease. renal histology in both siblings showed acute tubulointerstitial nephritis. yersinia pseudotuberculosis type vb was isolated from feces of one of them, antibodies to yersinia pseudotuberculosis type vb in their sera were elevated. the results of the present study suggest that acute renal failure complicating infections with yersinia pseudotuberculosis is due to a ...	1991	2032394
zoonoses and other findings in hedgehogs (erinaceus europaeus): a survey of mortality and review of the literature.	a survey of mortality in hedgehogs (erinaceus europaeus) was carried out between july 1976 and november 1986. most were from norfolk. of the 74 examined, 35 (47.3 per cent) were road casualties, one of which yielded salmonella typhimurium phage type (pt) 104. of the remaining 39, 13 (33.3 per cent) had salmonellosis due to s enteritidis pt 11. this organism, which appears to be common and widespread in hedgehogs in england was found in 10 separate incidents. the only other zoonosis was ringworm ...	1991	2035215
[yersinia and campylobacter food poisoning].		1991	2038688
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial.		1991	2040194
meiotic drive of t haplotypes: chromosome segregation in mice with tertiary trisomy.	the properties of the t haplotypes, specific mutant states of the proximal region of chromosomes 17 in the house mouse, are of continuing interest. one such property is increased transmission of the t haplotype by heterozygous t/+ males to offspring. using the reciprocal translocation t(16;17)43h we have constructed males with tertiary trisomy of chromosome 17 (+t43/+ +/rb7+) carrying the robertsonian translocation rb(16.17)7bnr. only the progeny of these males which had inherited either t43/+ o ...	1991	2040454
yersinia species isolated from sheep with enterocolitis.	in 40 submissions to the regional veterinary laboratory (rvl) wagga wagga from sheep in southern new south wales from 1981 to 1989, 53 isolates of yersinia sp were recovered from 45 sheep in 37 flocks. of 53 isolates, 26 were identified as y. pseudotuberculosis, 20 as y. enterocolitica, 5 as y. intermedia and 2 as y. frederiksenii. twelve isolates of y. pseudotuberculosis tested in the slide agglutination test all belonged to serotype iii. the 20 y. enterocolitica isolates were categorised bioch ...	1991	2043083
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility.		1991	2044514
fumonisin mycotoxins and equine leukoencephalomalacia.		1991	2045319
intestinal carriage of yersinia pseudotuberculosis by wild birds and mammals in japan.	fecal specimens were obtained from wild birds and mammals in the eastern part of shimane prefecture, japan, an area where serotype 1b, 2b, 3, and 4b strains of yersinia pseudotuberculosis were found to be prevalent in humans. each of 869 animals, including 259 wild birds and 610 wild mammals, was screened for yersiniae. a total of 37 strains of y. pseudotuberculosis were isolated from 34 (5.6%) mammals, including 23 raccoon dogs (nyctereutes procyonoides), eight deer (cervus nippon), two hares ( ...	1991	2059038
antibodies against dengue viral proteins in primary and secondary dengue hemorrhagic fever.	antibodies against dengue viral proteins were demonstrated in sera from dengue-infected patients by polyacrylamide gel electrophoresis, western blotting, and enzyme immunoassay. primary dengue cases showed low titers of igg class antibodies to envelope (e) proteins and two non-structural proteins, ns3 and ns5, in sera collected during the convalescent phase. secondary dengue- infected patients always demonstrated igg antibodies to e proteins in sera collected during the acute phase, and high tit ...	1991	2063952
[the possible mechanisms of the adaptation of microbial populations to low positive temperatures].	biologically low temperature was studied for its effect on the survival of populations and their biochemical and molecular-genetical state. the research was carried out on yersinia pseudotuberculosis and shigella sonnei models. it is established that the process of low-temperature adaptation of the populations may occur either at the level of the whole population with participation of inducible cold isoenzymes or at the level of a part of population, the temperature being a selection factor of t ...	1991	2067418
tyrosine phosphate hydrolysis of host proteins by an essential yersinia virulence determinant.	the plasmid-encoded yoph protein is a protein-tyrosine phosphatase (ptpase; ec 3.1.3.48) that is essential for yersinia virulence. we have investigated the molecular basis for the role of ptpase activity in yersinia pathogenesis. allelic recombination was employed to introduce a defined mutation into the yoph plasmid gene. conversion of the essential cys-403 to ala in the catalytic domain of the protein abolished yoph ptpase activity and significantly reduced the virulence of yersinia pseudotube ...	1991	1705028
analysis of the v antigen lcrgvh-yopbd operon of yersinia pseudotuberculosis: evidence for a regulatory role of lcrh and lcrv.	virulent yersinia species possess a common plasmid that encodes essential virulence determinants (yops) which are regulated by the extracellular stimuli ca2+ and temperature. the v antigen operon was recently shown to be involved in the ca2(+)-regulated negative pathway (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). we show here that the v antigen-containing operon of yersinia pseudotuberculosis is a polycistronic operon having the gene order lcrgvh-yopbd. dna sequencing analys ...	1991	1705541
an o antigen can interfere with the function of the yersinia pseudotuberculosis invasin protein.	escherichia coli strains harbouring the yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. we show here that this property is not shared by all enteric bacteria, since shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. mapping studies showed that the region of the chromosome responsible for this phenotype includes rfab, a locus involved in the production of o antigen. s. flexneri ...	1991	1710312
[the use of growth stimulants in the bacteriologic diagnosis of yersinia].	the effects of k. s. karpuzidi's stimulants (0.5-1 percent) and of para-amino benzoic acid (0.1-05 mm) on the growth and multiplication of 3 yersinia pseudotuberculosis, 1 y. enterocolitica strains, and e. coli nonpathogenic strain were examined. karpuzidi's stimulant was found to selectively stimulate yersinia cell growth and inhibit escherichia development in conditions unfavorable for these bacteria. para-amino benzoic acid intensively stimulated the growth of both yersinia and escherichia. w ...	1991	1710732
eric sequences: a novel family of repetitive elements in the genomes of escherichia coli, salmonella typhimurium and other enterobacteria.	we describe a family of highly conserved, enterobacterial repetitive intergenic consensus (eric) sequences, 14 of which have been identified in escherichia coli and salmonella typhimurium and a further three in other enterobacterial species (yersinia pseudotuberculosis, klebsiella pneumoniae and vibrio cholerae). eric sequences are 126 bp long and appear to be restricted to transcribed regions of the genome, either in intergenic regions of polycistronic operons or in untranslated regions upstrea ...	1991	1713281
[molecular and cellular bases of shigella flexneri virulence].	shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ...	1991	1742620
susceptibility of wild mice to yersinia pseudotuberculosis and yersinia enterocolitica.	laboratory-born progeny and adult populations of old world woods mice (apodemus speciosus) trapped in a certain area where yersinia pseudotuberculosis was prevalent among wild animals, were challenged with y. pseudotuberculosis and yersinia enterocolitica to determine the ecology of yersinia in wild mice. the laboratory-born progeny population was associated with infection with y. pseudotuberculosis but not with y. enterocolitica serotype o:3 and was found to be nearly 200 times more susceptible ...	1991	1755925
the nucleotide and deduced amino acid sequence of the cationic 19 kda outer membrane protein omph of yersinia pseudotuberculosis.	the omph proteins of enteric bacteria are recently described, small (16 kda), cationic outer membrane proteins. because a yersinia pseudotuberculosis cell envelope protein of this size has been found to cross-react serologically with the human histocompatibility antigen hla-b27 (b*2701), the sequence of y. pseudotuberculosis omph was determined by sequencing the gene region which encodes mature omph. a protein consisting of 143 amino acid residues was found. it was 96% homologous with the omph o ...	1991	1756172
yersiniosis in free-ranging muskoxen on banks island, northwest territories, canada.	sixty-seven muskox (ovibos moschatus) carcasses, 53 skeletal remains and two sick muskoxen were seen during an aerial survey of the thomsen river region, northern banks island, northwest territories, canada in late july 1986. complete necropsies were performed on 29 muskoxen estimated to have died within the previous 3 to 5 days. twenty were diagnosed with acute yersiniosis due to yersinia pseudotuberculosis serotype 1b and were in excellent body condition. a diagnosis could not be made on seven ...	1991	1758017
[an experimental study of yersinia in plants].	y. enterocolitica and y. pseudotuberculosis have been experimentally found to penetrate into plants (cabbage, salad, pea, oat) from infected soil and water and to remain in different plant organs (roots, seeds, leaves) up to 30 days (the term of observation). the processes of s-r dissociation in yersinia cultures isolated from plants, as well as changes in some enzymatic properties, have been established. in the test on infusoria the cytotoxic action of strains isolated from plants has been note ...	1991	1759521
afterloading increases the left ventricular end-systolic force-length relation slope in dogs.	we evaluated the effects of pressure loading produced by gradual aortic occlusion on left ventricular (lv) myocardial contractility by assessing changes in the slope of the lv end-systolic force-diameter (fes-des) relation. eleven adult mongrel dogs were prepared with ultrasonic crystals for measuring lv diameter and a micromanometer for measuring lv pressure. preload was decreased by vena caval occlusion (vco), and afterload was increased by aortic occlusion (aoo). the slopes (ec) and extrapola ...	1991	1774832
yersinia in effluents from the food-processing industry.	yersinia enterocolitica and yersinia pseudotuberculosis are current sources of pathogenic strains in humans and animals. yersiniae infections occur throughout the world, but are most prevalent in regions with moderate and subtropical climates. in australia, central europe and north america, cases of human infections with yersinia enterocolitica now rank in third place. the food-processing industry may influence the epidemiological situation in different ways. effluents which contaminate the envi ...	1991	1782430
[proline specific peptidases and their specific inhibitors].	several proline specific peptidases were newly isolated from mammalian organs, plants and microorganisms, and their enzymatic properties characterized. the genes of prolyl endopeptidase, aminopeptidase p and proline iminopeptidase from some microorganisms were cloned and their nucleotide sequences determined. by high expression of these genes in escherichia coli, the enzymes became possible to be used for the industrial application as well as its basic research. novel inhibitors specific for the ...	1991	1783984
recognition by peptide mapping of three different structural groups of outer membrane protein yop-1 of yersinia enterocolitica and yersinia pseudotuberculosis.	the structural relation of yop-1 of "european" and "american" yersinia enterocolitica serotypes o:3, o:9, o:5,27, and o:8 and o:20, respectively, and y. pseudotuberculosis serotypes i, ii, and iii was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using staphylococcus aureus protease v8. apparent molecular weights of yop-1 ranged from 206,000 (o:3) to approx. 180,000 (o:8). according to their respective peptide maps yop-1 of the "european" and "american ...	1991	1784270
[the role of a plasmid with a molecular weight of 82 md in the modulation of the action of yersinia pseudotuberculosis on the proliferative activity of murine lymphoid cells].		1991	1787842
[the gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of yersinia pseudotuberculosis].	genetic analysis of 19 standard strains belonging to 6 yersinia species (y. pestis, y. pseudotuberculosis, y. enterocolitica, y. kirstensenii, y. frederiksenii, y. intermedia) revealed that gene typing by the method of polymerase chain reaction (pcr) with the use of universal primers permitted the identification of species in bacterial cultures by pcr patterns and the determination of y. pseudotuberculosis serovars within 4 hours. by this method 23 y. pseudotuberculosis strains (serovar 1), earl ...	1991	1789029
real-time monitoring of slow-wave sleep by electroencephalogram variance.	based on statistical variance as an index of electroencephalogram (eeg) parameters, we monitored slow-wave sleep in both humans and rats in real time and on-line with a widely used personal computer. this eeg variance method may be a useful tool to carry out biological rhythm research, including sleep studies.	1991	1794153
aspirin-like drugs, ethanol-induced rat gastric injury and mucosal eicosanoid release.	the effect of oral administration of various non-steroidal antiinflammatory drugs on ethanol-induced rat gastric injury and mucosal release of leukotriene c4, 6-keto-prostaglandin f1 alpha and 15-hydroxy-5,8,11,13-eicosatetraenoic acid was investigated. it was found that besides sodium salicylate and high doses of aspirin, other salicylate-type drugs, such as diflunisal, 4-aminosalicylic acid, 2,4-dihydroxybenzoic acid and methyl salicylate, and several non-acidic compounds, such as proquazone, ...	1991	1794414
viability and ultrastructure of equine embryos following culture in a static or dynamic system.	the viability and ultrastructure of equine embryos were assessed following culture in a static or perifusion system. the percentage change in diameter was greater (p less than 0.025) for embryos in the static treatment (71%) than in the perifusion treatment (33%). fluorescein diacetate (fd) scores, the percentage of fluorescing cells (fc) and fluorescent intensity (fi), also were greater (p less than 0.05 and p less than 0.01) following static culture than for embryos cultured in the perifusion ...	1991	1795284
memory modulation by brain benzodiazepines.	1. recent evidence indicates that post-training memory processes are down-regulated by benzodiazepine/gaba-a systems in the amygdala, septum and hippocampus. habituation and avoidance learning are accompanied by a decrease of benzodiazepine-like immunoreactivity in the three structures, explainable by a release of benzodiazepines. immediate post-training microinjection of the benzodiazepine antagonist flumazenil into the hippocampus enhances retention of habituation. the post-training administra ...	1991	1797279
memory modulation by brain benzodiazepines.	1. recent evidence indicates that post-training memory processes are down-regulated by benzodiazepine/gaba-a systems in the amygdala, septum and hippocampus. habituation and avoidance learning are accompanied by a decrease of benzodiazepine-like immunoreactivity in the three structures, explainable by a release of benzodiazepines. immediate post-training microinjection of the benzodiazepine antagonist flumazenil into the hippocampus enhances retention of habituation. the post-training administra ...	1991	1797279
[secondary immunodeficiency in pseudotuberculosis in man and in experiment].	surgical material from 55 children, from 2 to 15 years of age with an abdominal form of pseudotuberculosis (appendix, lymph nodes, ileum) and the immunogenesis organs (lymphoid tissue of the small intestine, lymph nodes, appendix, spleen and thymus) of 277 rabbits infected with virulent strains of the yersinia pseudotuberculosis i serovar at the dose of 10(8) bacterial bodies were investigated. the results testify to the disturbance of all parts of the immuno-phagocytic system in pseudotuberculo ...	1991	1799314