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inhomogeneous distribution of filipin-sterol complexes in the ciliary membrane of rat tracheal epithelium.pieces of rat trachea fixed in a glutaraldehyde solution containing 300 mum filipin, a sterol-specific polyene antibiotic (elias et al., '78), were freeze-fractured in order to study the distribution of cholesterol within the ciliary membrane. filipin-sterol complexes, recognizable as 25-30-nm protrusions on fracture faces, appeared densely and uniformly distributed over most of the ciliary membrane, but were absent from the region of the ciliary necklace. it is possible, therefore, that the cil ...1979517447
histologic diagnosis of diseases of malabsorption.the diagnoses which may be arrived at by examination of peroral small bowel mucosal biopsy specimens are presented. celiac sprue, unclassified sprue (refractory sprue), infectious gastroenterititis, stasis syndrome and kwashiorkor have a severe mucosal lesion. other clinical conditions are required to establish the diagnosis in these diseases. a number of diseases have specific diagnostic features. included are whipple's disease, abetalipoproteinemia, collagenous sprue, primary intestinal lympho ...1979517556
[effects of ionizing radiations on the ultrastructure of bursa of fabricius].the alterations induced in the lymphoid and epitelial cells of the bursa of fabricius in chicks exposed at different doses of gamma rays mainly occur during the interfase. the extent of these alterations appears reduced in comparison with that observed in the chick thymus treated in the same way.1979518763
studies on selective induction of lysosomal enzyme activities in mouse peritoneal macrophages. 1979522087
[problems of the disease due to mycobacteria other than tubercle bacilli: history of studies and unsolved problems (author's transl)]. 1979522347
experimental granulomatous inflammation: ii. effect of injection of intact and disrupted killed tubercle bacilli into freund adjuvant-sensitized cavies.the reaction of freund adjuvant-immunized cavies to subsequent injection of mycobacterium tuberculosis h37ra is confirmed as an immunologically mediated phenomenon. ultrasonic disruption of mycobacteria to be injected as "challenge" markedly increases the intensity of the consequent 24-48 h reaction and subsequent fibrosis in freund adjuvant-sensitized cavies and has an apparently inhibiting effect on the extent of subsequent granuloma formation with marked reduction of the density of epithelioi ...1979523183
[new cases of opisthorchiasis and their anatomopathological picture in cats in warmia]. 1979524889
insulin responsiveness of adipose tissue from normal weight subjects with early diabetes.in normal weight subjects, classified by a 2-h glucose infusion test as having normal (11), borderline (3) or pathological (9) carbohydrate tolerance (cht), subcutaneous adipose tissue was removed under intracutaneous anesthesia by surgical biopsy. the biological responsiveness of isolated adipocytes as well as adipose tissue fragments measured as incorportion of (1-14c) glucose into co2 or triglycerides was studied in the absence or presence of different insulin concentrations. in persons with ...1979525210
serially etched shark enameloid observed by incident light microscopy.longitudinal and transverse tooth sections of isurus oxyrinchus were serially etched in 2.6% nitric acid. the changing optical properties of the etched surfaces were observed during the serial etchings, and the descent of the enameloid surfaces was measured. shark enameloid seems to be less effectively etched by acid than human enamel; this difference may be due to differences in solubility between fluorapatite and hydroxyapatite. most of the information regarding the structure of the enameloid ...1979525241
hybridization and heat tolerance in rats.buffalo and fisher inbred rats and their f1--f4 hybrids were exposed to heat, 96 degrees f and 30% humidity, at 35 days of age. fisher rats which are of lighter weight survive significantly longer than buffalo rats which are heavier. body weight was significantly depressed in all hybrids. only in f2 hybrids was heat tolerance similar to that of the fisher inbreds. in all other hybrids, it was lower. no heterosis was found under the conditions used.1979525249
toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography. 1979526270
the relationship between early automatic and late voluntary compensatory responses to muscle stretch in the long flexor of the human thumb [proceedings]. 1979529071
effect of vibration on the mechanical resistance of the human forearm to small imposed sinusoidal movements [proceedings]. 1979529079
disaccharide competition with hexose transport in the rat perfused liver [proceedings]. 1979529099
adrenergic stimulation of mucus secretion in the human bronchus [proceedings]. 1979529116
reflex action of amplitude-modulated vibration in man [proceedings]. 1979529124
effect of sodium on the absorption of glucose and galactose across the rat jejunum measured in vivo [proceedings]. 1979529125
nucleic acid and protein metabolism in the pancreas, spleen, thymus, lung, cardiac and skeletal muscle and lens of undernourished female rats. 1979533871
the effect of temporary exercising of a joint during an immobilization period: an experimental study on rabbits.the right hind limbs of adult rabbits were immobilized for 5 weeks. the rabbits were then allowed to move freely for 8 weeks. the rabbits of the control group were immobilized uninterruptedly. in a second group of rabbits the plaster was removed during the immobilization period 6 days a week for 5 minutes for careful exercise and in a third group of rabbits, for violent exercise. the temporary careful exercise was of no benefit and was sometimes harmful, and the violent exercise was usually inju ...1979535243
euthanasia: a serene voyage to death?modern western culture regards death as a threatening enemy, whereas the ancients, as is the case in eastern philosophy, recognized both the fight with, and the releasing surrender to death. dying is quite often a serene voyage, be it through old age, sickness, was or even suicide. attentive listening to those who face the termination of incurable disease reveals the voice of enlightenment: 'euthanasia' also means 'clarified dying'. an attitude like this alleviates our recoil and assists in a su ...1979535362
antiplague antibodies against yersinia pestis fraction-i antigen in serum from rodents either experimentally infected or captured in harbor areas of japan, 1971--1977.antiplague indirect hemagglutination tests were investigated with yersinia pestis fraction-i antigen and sera from experimentally infected rodents. the same tests with sera treated with acetone or acetone-2-mercaptoethanol were performed in parallel. technical conditions were determined to insure the specificity and reproducibility of the reactions. utilizing the methods studied, we carried out serological surveillance of plague with sera from wild rats (rattus rattus and r. norvegicus) captured ...1979537520
vein grafts in microvascular surgery.the judicious use of vein grafts will lessen the incidence of thrombosis in microvascular surgery and help to overcome problem of vessels loss. vein grafts are readily available and do not pose any major disadvantages.1979539801
identification of a new allele of es-i segregating in an inbred strain of mice.a new allele of es-1, designated es-1e, has been identified in the mouse. this allele was discovered segregating among the progeny of a strain dba/2j male and is apparently the result of a spontaneous mutation within this strain. genetic analyses have shown that this mutation is heritable and, further, that both heterozygous and homozygous progeny are viable and fertile. to date, no discernible deleterious effects have been identified as associated with this mutation.1979540008
identification of a new allele of es-i segregating in an inbred strain of mice.a new allele of es-1, designated es-1e, has been identified in the mouse. this allele was discovered segregating among the progeny of a strain dba/2j male and is apparently the result of a spontaneous mutation within this strain. genetic analyses have shown that this mutation is heritable and, further, that both heterozygous and homozygous progeny are viable and fertile. to date, no discernible deleterious effects have been identified as associated with this mutation.1979540008
[the development and testing of an electronically controlled light sequence bar for the electro oculographic study of the pursuit movement of the human eye (author's transl)]. 1979540121
[characteristics of human neutrophils obtained by the chamber method of "skin window"].viability, morphology and function of healthy human cells migrated into the chamber through the "skin window" were studied. after 18 to 20 hours there were 42.0 +/- 5.3 (x 10(6))/cm2 viable cells characterized by a high content of mature neutrophils (98.6 +/- 0.6%). normal reactivity of migrated neutrophils was observed using the nitroblue tetrazolium reduction test. the chamber variant of the "skin window" method is recommended as a simple physiological technique for preparing pure populations ...1979540158
[human phrenic nerve: analysis of nerve fibre types on the base of their acetylcholinesterase activity (author's transl)].as marked differences in the acetylcholinesterase (ache)-activity of myelinated nerve fibres of ventral and dorsal spinal roots can be found also in human post mortem material (zenker et al. 1978), the karnovsky-method for histochemical demonstration of ache-activity has been used for differentiation of motor and sensory fibres in the human phrenic nerve. in cross sections of the phrenic nerve 1--2 cm above its entrance into the diaphragm, after an incubation period of 24 hours, 86% of the medul ...1979543560
liver involvement in essential mixed cryoglobulinemia.twenty-one of 30 patients with essential mixed cryoglobulinemia (emc) had evidence of liver involvement. the liver disease was characterized by the absence of clinical symptoms, hepatosplenomegaly, mild elevation of enzymes, abnormal bsp retention and low albumin levels. histology, available in 12 patients, showed either chronic persistent or chronic active hepatitis or liver cirrhosis; 44% of the patients had hbsag or hbsab in sera and/or cryoglobulins, confirming the high frequency of exposure ...1979545644
plague antibody in large african mammals.plague hemagglutinating antibodies to a titer of 1:1,024 were demonstrated in 6.6% of buffalo and 0.3% of elephant sera tested 1 year after a plague epidemic in the same area.1979546794
correlation between gross observations of tumors and neoplasms diagnosed microscopically in carcinogenesis bioassays in rats.microscopic diagnoses of spontaneous and induced neoplasms in f344 rats in two carcinogenesis bioassays were correlated with the gross observations made at the time of necropsy. the results for a negative and a positive compound with reference to carcinogenicity, indicate that of the gross descriptions indicative of tumors, 37 percent and 24 percent respectively, were not diagnosed as neoplasms microscopically. however, some of the gross lesions were diagnosed histologically as nonneoplastic les ...1979547015
crown gall: economic importance and control.many plants of economic importance are possible hosts for agrobacterium tumefaciens, the causal organism of the crown gall tumor disease. damage has been reported on stone fruit (australia), vineyard (hungary, bulgaria), lettuce (brasil) ... in western-europe, crown gall seems to be of less economic importance for plants growing in the open air. however, plants cultivated in greenhouses have a greater chance to be tumorized, because of the more favourable circumstances for tumor induction. moder ...1979549390
vestibular influences on the cat's cerebral cortex. 1979551452
experimental gingivitis in odu plaque-susceptible rats. iii. toxic activity of the rat dental plaque.the toxicity tests of the dental plaque from odu plaque-susceptible rats showed strong lethal effect on mice, and abscess forming effect on guinea-pigs. bacterial cells isolated from the rat dental plaque also showed strong toxicity on both animals and capillary permeable activity on rabbits. among these bacterial cells, corynebacterium showed the strongest toxic effects on these animals. these facts suggested an important role of the dental plaque on initiation and development of gingivitis and ...1979289757
[experimental infection of biomphalaria glabrata with schistosoma mansoni in the presence of helisoma duryi]. 1979262314
[efficacy of various culture media in the preservation of yersinia pestis strains]. 1979262317
[brain aging. ultrastructural study of changes in man and laboratory animals].the authors present a study on ultrastructural changes observed in aged human and animal brain. these changes are analyzed and compared with those described in classic optic microscopy and with those observed in experimental models of the same lesions.1979262351
[prevention and therapy of viral hepatitis]. 1979253774
epidemiologic survey of sylvatic plague by serotesting coyote sentinels with enzyme immunoassay.the geographic distribution and areas of high sylvatic plague activity in california were verified by using coyotes (canis latrans) as sentinel animals. antibody levels against yersinia pestis were tested using the enzyme-labelled antibody (ela) test and the microtiter passive hemagglutination and hemagglutination inhibition. a survey using the ela test indicated that the overall antibody prevalence among 143 coyotes was 21%. by geographic regions, the highest antibody prevalence was 27% among c ...1979382839
site-specific recombination leading to the integration of phages p1 and p7. 1979385221
u.s. court stops hew's selection of intermediary. 1979387571
[nuclease-antibodies in infections with serratia marcescens (author's transl)].a total of 467 presumable healthy men of age eighteen yielded titres of anti-serratia-nuclease that ranged from 1:4 to 1:6. one third of 56 patients, who had been infected with serratia marcescens (urinary-, wound-, respiratory-infections, and bacteremia) yielded raised anti-nuclease titres, which amounted maximaly 1:16. the diagnostic utility of this serological test was discussed.1979387574
synthesis and properties of fluorescent nucleotide substrates for dna-dependent rna polymerases.a new class of fluorescent nucleotide analogs which contain the fluorophore 1-aminonaphthalene-5-sulfonate attached via a gamma-phosphoamidate bond has been synthesized. both the purine and pyrimidine analogs have fluorescence emission maxima at 460 nm. cleavage of the alpha-beta-phosphoryl bond produces change in both the absorption and fluorescence emission spectra. the fluorescence of the pyrimidine analogs is quenched; cleavage of the alpha-beta-phosphoryl bond of the utp analog produces abo ...1979387781
the association of var1 with the 38 s mitochondrial ribosomal subunit in yeast. 1979387793
api and minitek systems in identification of clinical isolates of anaerobic gram-negative bacilli and clostridium species.a comparison of the api and minitek methods of biochemical testing was made on a variety of anaerobic bacteria. although api and minitek results were not compared to more standardized or conventional procedures of identification, multiple repeat testing of the two systems was done on routine clinical isolates and known organisms to determine (i) whether the reactions were reliably consistent, (ii) the ease of reading the two systems with respect to the frequency of questionable results, and (iii ...1979387809
primary plate identification of group a beta-hemolytic streptococci utilizing a two-disk technique.a two-disk system is described which allows primary plate identification of group a beta-hemolytic streptococci. group a beta-hemolytic streptococci could be visualized on primary throat culture plates by using trimethoprim-sulfamethoxazole to inhibit normal flora. in the heavily inoculated area of trypticase soy agar plates containing 5% sheep blood, a 25-microgram/ml trimethoprim-sulfamethoxazole disk was placed contiguous to a 0.04-u bacitracin disk. a total of 259 throat specimens were exami ...1979387811
metabolism of arachidonic acid in ionophore-stimulated neutrophils. esterification of a hydroxylated metabolite into phospholipids.[14c]arachidonic acid incubated with human neutrophils was esterified into phospholipids and triglycerides. stimulation of these labeled neutrophils with ionophore a23187 (2 microm) results in release of [14c]arachidonate from phospholipid and its metabolism to prostaglandin e2 and 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-hete), a lipoxygenase product. the released arachidonate is also metabolized to a polar lipid of unknown composition here disignated compound a. 5-hete was found to be rele ...1979387819
serum opsonic activity in rodent malaria: functional and immunochemical characteristics in vitro.the functional and immunochemical characteristics of serum opsonic activity in rodent malaria were examined in the present study. schizont- and late trophozoite-enriched populations of plasmodium berghei-infected red blood cells (irbc) were isolated on a ficoll density-gradient and used in an in vitro phagocytosis system composed of serum and monolayer cultures of rat peritoneal macrophages. hyperimmune serum augmented the phagocytosis of irbc to a greater degree than did nonimmune serum. when e ...1979387873
[inductions of adjuvant arthritis in rats and footpad swelling reaction in mice by injection with the purified cell wall fraction from in vitro grown mycobacterium lepraemurium hawaii (author's transl)]. 1979389918
human plasma angiotensinogen: a review of purification procedures.the current status of the purification and characterization of human angiotensinogen is reviewed. one problem encountered in the past has been the copurification of a protein with similar porperties. this protein has tentatively been designated alanine-protein. an efficient separation of angiotensinogen and alanine-protein was obtained on a zinc chelate column. alanine-protein has been purified and its amino acid and carbohydrate composition determined. the cooh-terminal amino acid and the nh2-t ...1979390363
[properties of plague microbe revertants obtained from l forms]. 1979390929
isolation of pathogens other than yersinia pestis during plague investigations.from 1975 to 1978, 37 isolates of pasteurella multocida, 1 of salmonella enteriditis, and 5 of francisella tularensis were recovered from 42 mammalian specimens and 1 flea pool submitted for examination for evidence of infection with yersinia pestis. most of the specimens were collected during investigations of either a human plague infection or a reported epizootic among rodent populations. all specimens were of species regularly or occasionally involved in plague or tularemia cycles in nature ...1979392123
lung compliance and lung morphology following artificial ventilation in the premature and full-term rabbit neonate.rabbit neonates delivered on day 27--30 of gestation were subjected to intermittent positive pressure ventilation (ippv) for 60 min. tidal volume was standardized to 10 ml/kg, and the insufflation pressure required to maintain this tidal volume was recorded. the quasistatic compliance of the lung-thorax system, corrected for variations in body weight, was significantly lower in animals delivered on day 27--28 than in those delivered on day 29--30. immature animals with low lung-thorax complianc ...1979394314
[the molecular biology of aging -- 15: structure and function of the genetic apparatus in aging. ii. synthesis and metabolism of rna].with aging in different organs there are similar or tissue specific changes of the ribonucleic acids in respect of the quantity, the spectrum of different rna species and the metabolism of rna. among these processes the template function of the chromatin and the behaviour of the rna polymerases are important influencing factors. in aging the sensitivity of the rna synthesis against hormonal stimulation is decreased in the most cases. a survey about the results of many in vivo and in vitro experi ...1979395770
genetic modification of macrophage functions in relation to antibody responsiveness and resistance to infection. 1979397751
the preparation and characterization of a cell-free system from saccharomyces cerevisiae that translates natural messenger ribonucleic acid.a cell-free protein-synthesizing system has been prepared from saccharomyces cerevisiae by differential centrifugation of lysed spheroplasts. the preparation, a modified 100,000 x g supernatant fraction, contains ribosomes and monosomes, ribosomal subunits, translation factors, and aminoacyl-trna synthetases, but no polysomes. after removal of small amounts of remaining mrna with micrococcal nuclease, protein synthesis is stringently dependent on the addition of mrna, as well as amino acids and ...1979374404
isolation of trypanosoma cruzi from the blood of infected mice by column chromatography.trypomastogotes of three strains of trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of deae cellulose for removal of white cells and platelets. an average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. protein and carbohydrate assays of the separated organisms revealed significant differences between the tulahuen, a reticulotropic strain, ...1979375218
[evaluation of fluoride content in modena water].the authors confirm the importance of fluoroprophylaxis in the prevention of dental caries. they analyze the main methods of fluoroprophylaxis pointing out the systemic fluoroprophylaxis, effected by means of water fluoruration, as the best one for a valid prevention of dental caries on a large scale. in view of such a kind of fluoroprophylaxis in our country the authors considered quite interesting to effect a research about the amount of fluoride in the waters of the district of modena. the wh ...19792395770
[influence of "prudent" diet and clofibrate upon apolipoprotein b in hyperlipoproteinemia type iia (author's transl)].if patients with hyperlipoproteinemia type iia are treated with diet and additionally over a period of two weeks with 2 grams of clofibrate daily, the cholesterol-level in the serum decreases. this decrease is caused mainly by a reduction of the apolipoprotein b and the ldl-cholesterol in serum. if elevated cholesterol-levels in the serum are reduced through a "prudent" diet, the concentration of apolipoprotein b in serum remains the same. it is not fully understood which influence a diet, which ...1979216895
obstruction of the abdominal aorta by a primary retroperitoneal tumor.an angiographically demonstrated case of total occlusion of the abdominal aorta by a malignant retroperitoneal tumor is presented. surgical exploration revealed a diffuse tumor of the retroperitoneum involving the wall and lumen of the abdominal aorta and vena cava, respectively. histologic and ultrastructural evaluation of the tumor established a diagnosis of malignant fibrous histiocytoma. total occlusion of the abdominal aorta thus represents another angiographic finding in retroperitoneal tu ...1979218312
[evaluation of the results of 2-component serological reactions in plague depending on the quantitative relations between the complete and blocking components of the sample]. 19806155558
[determination of asparagine deaminase activity in identifying cultures of the causative agents of plague and pseudotuberculosis]. 19806157885
[interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)].gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ...19806104464
[case of the isolation of the causative agent of plague (yersinia pestis) from the isabelline wheatear (oenanthe isabellina temm.)]. 19806446677
[lysogeny by phages specific to yersinia pestis in bacteria of the microbiocenoses of wild plague-carrying rodents].for the first time lysogenic bacteria of the genus escherichia, spontaneously producing bacteriophages specific to the infective agent of plague, have been detected in the microbiocenoses of midday gerbils, plague carrier in the volga-urals natural focus. the morphological, cultural and biochemical properties of 22 lysogenic escherichia strains isolated from wild rodents, as well as the spectra of the lytic activity of temperate bacteriophages produced by the above-mentioned microorganisms have ...19806449813
[characteristics of microbial multiplication in parenterally infected xenopsylla cheopis (siphonaptera) fleas].being inoculated parenterally various microbes cause the fleas x. cheopis a stable infection which, as a rule, is preserved in experimental insects to the end of life. reproducing intensively listeria monocytogenes and salmonella typhimurium caused the death of all ectoparasites in 3 to 5 days. the increase in abundance of escherichia coli, yersinia pseudotuberculosis, y. enterocolitica and vaccine strains of y. pestis "ev" and francisella tularensis went on gradually and infected fleas lived up ...19806780964
classification and identification of bacteria by electrophoresis of their proteins. 19807025226
recommendations for the control of yersinia pestis infections. recommendations from the cdc.yersinia pestis, the etiologic agent of plague, is endemic in the western united states; 105 cases were reported between 1970 and 1979. plague may manifest in one of three clinical forms; bubonic, septicemic, or pneumonic. bubonic and septicemic plague represent relatively little risk for human-to-human transmission to contacts, although heavily infected secretions, such as drainage from a bubo, pose a theoretical risk, especially if they are aerosolized from a syringe during diagnohree clinical ...19806904395
chemical composition and biological activity of the yersinia pestis envelope substance.purification of the envelope antigen of yersinia pestis ev with passive hemagglutination activity is described. the purification procedure consisted of pancreatin digestion, chromatography on human erythrocyte stroma set on celite, and rechromatography on sephadex g-200. chemical, physical, and biological properties of this antigen were investigated. the results show the lipid-polysaccharide structure of the isolated antigen. the carbohydrate moiety of the galactolipid antigen consists of galact ...19807002800
plague in central java, indonesia.plague in man occurred from 1968 to 1970 in mountain villages of the boyolali regency in central java. infected fleas, infected rats, and seropositive rats were collected in villages with human plague cases. subsequent isolations of yersinia pestis and seropositive rodents, detected during investigations of rodent plague undertaken by the government of indonesia and the who, attested to the persistence of plague in the region from 1972 to 1974.since 1968, the incidence of both rodent and human p ...19806968252
potency of killed plague vaccines prepared from avirulent yersinia pestis.killed plague vaccines prepared from avirulent strains a1122 and ev76s of yersinia pestis were more effective in mouse potency tests than samples of plague vaccine, usp, prepared from killed y. pestis of the virulent strain 195/p. manufacture of vaccine from avirulent y. pestis would obviate requirements for the large containment facilities that are currently needed for producing plague vaccine, usp.19806975184
[patterns in the reactions between monovalent antigens and antibodies]. 19807192464
[pathology of experimental infection in domestic rodents using various strains of yersinia pestis]. 19807348290
[serological and experimental studies of plague in northeastern brazil]. 19807348812
correlation of autoagglutination and virulence of yersiniae.virulent strains of yersinia pestis, y. pseudotuberculosis and yersinia enterocolitica invariably autoagglutinated in tissue culture media when grown at 36 degrees c. avirulent strains did not possess this property.19807372804
from the center for disease control. trends in human plague in the united states. 19807373084
roles of metallic ions within an area endemic for the plague bacillus (yersinia pestis).there are observations that strongly suggest that the synthesis of specific factors of virulence in the plaque bacillus (yersinia pestis) depend on the level of a specific metallic ion(s). the varied roles of metallic ions in host-plague interactions are quite similar to the structural and catalytic roles of such ions in free-living macro-and micro-organisms. the antimicrobial power of mammalian fluids is depressed by low levels of fe and enhanced by an increase in the fe binding capacity of a s ...19807382883
virulence and viability of yersinia pestis 25 years after lyophilization.when equal volumes of 6% lactose and a broth culture of yersinia pestis were mixed before freezing, approximately 50% of the cells survived lyophilization and reconstitution on the following day. concomitantly, the number of viable cells per 50% lethal dose increased from about 16 to 125 organisms. on subsequent storage of the lyophilized cells under vacuum in glass ampoules at 4 degrees c for 25 years, more than 25% of the cells remained viable. when stored cultures were assayed immediately aft ...19807387153
plague in camels and goats: their role in human epidemics.in 1976, in a small, remote libyan village, one apparently sick camel was slaughtered and skinned, and the camel meat was distributed for human comsumption. a few days later, 15 villagers suffered a severe febrile illness. of the five individuals who had participated in the killing and dispensation of the camel, all were dead within four days. when samples of serum from nine of the remaining patients were examined, seven were found to be positive for plague as determined by the passive hemagglut ...19807391614
[cross immunity to plague and tularemia]. 19807395403
[ultrastructural changes in plague microbes of vaccinal strain eb and alveolar macrophages of guinea pigs during their interaction in vitro].aerosol immunization with plague vaccine eb was found to stimulate an increase in the number of free alveolar macrophages and to enhance their ingesting activity which correlated with an increase in specific antibody titers. immunological transformation was accompanied by ultrastructural changes in the cytoplasm and the nuclei of alveolar macrophages. the phagocytosis of microbes occurred by their adsorption onto the microphage surface, invagination and enclosing into the endocytic bubble having ...19807395404
plague virulence antigens from yersinia enterocolitica.the virulence of yersinia enterocolitica, biotype 2, serotype o:8, in mice is related to its ability to produce plague v and w antigens. v and w antigens in y. enterocolitica are shown to be immunologically identical to the previously described v and w antigens of yersinia pestis and yersinia pseudotuberculosis.19807399681
[histological study of xenopsylla cheopis fleas infected with the ulegeic subspecies of the plague microbe, yersinia pestis ulegeica].the plague microbe of the ulegei subspecies is able to cause the stable infection of fleas of x. cheopis, which is accompanied by the formation of "block" in most individuals. the preservation of the plague agent in these ectoparasites is characterized by its variation in the number that undergoes cyclic changes depending on the feeding rate and digestion. the bloodsucking is accompanied by the excretion of some microbes and by the reproduction of the remaining ones in the midgut and proventricu ...19807402697
[pronase study of pestin pp structure and function]. 19807405449
[action of ampicillin on the plague microbe (y. pestis ev) according to electron microscopic data]. 19807406460
[fatty acid makeup of bacteria in the genus yersinia]. 19807412606
[transformation of the agent of plague into the l-form]. 19807415679
studies on yersinia antigens. 19807448457
outer membrane protein composition of yersinia pestis at different growth stages and incubation temperatures.the protein composition of the outer membrane of yersinia pestis grown at 26 and at 37 degrees c was examined. the outer membrane was isolated by isopycnic sucrose density centrifugation, and its degree of purity was determined with known inner and outer membrane components. using two-dimensional gel electrophoresis, we identified a large number of heat-modifiable proteins in the outer membrane of cells grown at either incubation temperature. one-dimensional sodium dodecyl sulfate-polyacrylamide ...19807204338
the general practitioner and the endodontist.one of the rewards of specialty practice is the opportunity to work with a great many referring dentists. the camaraderie is good, the friendships that develop are satisfying and long lasting. the specialist's goals are the same as those of the general practitioner: to provide the highest level of care possible in the best interest of their shared patient. this is best accomplished by open and effective communication between the general practitioner, the endodontist, and their mutual patient.1980294398
[oblique transcranial lift of zygomatic arch for presentation of fractures of the zygomatic process of the temporal bone (2)]. 19802395990
[a pacemaker for experimental use (author's transl)]. 1980550983
ovulation induction with bromocriptine (cb154) in patients with hyperprolactinaemia. 1980273419
plasmids in yersinia pestis.pesticinogenic and ca2+-dependent strains of yersinia pestis harbored plasmids of about 6 and 45 megadaltons, respectively. in addition, most isolates examined possessed a cryptic 65-megadalton plasmid.19817216478
occurrence of yersinia enterocolitica in wild animals.yersinia species were isolated from 16 of 495 small wild animals and from 1 of 38 foxes. the animals were trapped in seven regions of hokkaido, japan. of the 17 strains isolated, 9 were yersinia enterocolitica o6; 2 were y. enterocolitica o5a; 1 was y. enterocolitica, o4; 1 was y. enterocolitica o9; 1 was yersinia pseudotuberculosis ivb; and 3 were sucrose-negative strains. yersinia pestis was not isolated. the o6 organism was most prevalent in large red-back mice (clethrionomys rufocanus bedfor ...19817224628
plague in a neonate.more than half of the cases of plague in the united states are diagnosed in children. in the 1970s, 105 cases were reported, a 350% increase over the 1960s. plague should be considered in the differential diagnosis of children from the western united states who have sepsis, especially those with lymphadenopathy. complications of plague include meningitis, metabolic encephalopathy, abscess, and pneumonia. with appropriate treatment, prompt clinical response usually occurs. careful isolation and e ...19817234765
[utilization and fermentation of 2-ketogluconate by "enterobacteriaceae" (author's transl)].two methods were compared: 1) the ability to use 2-ketogluconate (2-kg) as source of carbon and energy in defined medium, and 2) the ability to produce acid by fermentation of 2-kg (0.5 g/100 ml) in peptone water with phenol red. results of growth on, and acid production from, 2-kg were identical. a total of 990 strains was studied. no strain of escherichia coli, shigella (4 species), salmonella (4 sub-genera), yersinia pestis, y. pseudotuberculosis, edwardsiella (2 species), proteus vulgaris, p ...19817235456
essential virulence determinants of different yersinia species are carried on a common plasmid. 19817243971
[immunologic activity of the "murine" toxin of the plague microbe in experiments on animals]. 19817245966
[antibiotic sensitivity of strains of the plague microbe isolated from foci in the caucasus and transcaucasia].antibiotic sensitivity of 91 strains of the plague microbe isolated in armenia, azerbaijan, daghestan, georgia, kabardino-balkaria and the stavropol region in 1977-1979 from the rodents and their ectoparasites was studied with the method of serial dilutions in the hottinger agar. the following antibiotics were tested: streptomycin, erythromycin, tetracycline, methicillin, gentamicin, levomycetin, monomycin, lincomycin, ristomycin, rifampicin, kanamycin, doxycyclin and polymyxin m. all of the str ...19817259143
[ultrastructural characteristics of the l transformation process of the plague microbe]. 19817260205
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