Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| regio- and stereospecific oxidation of 9,10-dihydroanthracene and 9,10-dihydrophenanthrene by naphthalene dioxygenase: structure and absolute stereochemistry of metabolites. | the oxidation of 9,10-dihydroanthracene and 9,10-dihydrophenanthrene was examined with mutant and recombinant strains expressing naphthalene dioxygenase from pseudomonas putida (ncib 9816.4. salicylate-induced cells of p. putida strain 9816/11 and isopropylthiogalactopyranoside-induced cells of escherichia coli jm109(de3)(pdtg141) oxidized 9,10-dihydroanthracene to (+)-cis-1r,2s)-1,2-dihydroxy-1,2,9,10-tetrahydroanthracene (> 95% relative yield; > 95% enantiomeric excess) as the major product. 9 ... | 1996 | 8795226 |
| increased mutagenesis mediated by cloned plasmid cam-oct genes: potential for expanding substrate ranges of pseudomonas spp. | twenty-five kilobases of pseudomonas plasmid cam-oct dna encoding a dna repair gene(s) was cloned into the broad-host-range vector pvk100. the presence of the cloned genes increased the isolation frequency of pseudomonas putida derivatives capable of using ethyl lactate or 3-methyl-3-buten-1-ol as their carbon source 15- and 8-fold, respectively, after uv irradiation. ethyl lactate-utilizing strains expressed a novel intracellular hydrolase. | 1996 | 8795249 |
| crystallization and preliminary x-ray analysis of salicylate hydroxylase from pseudomonas putida s-1. | apo-salicylate hydroxylase from pseudomonas putida s-1 has been crystallized by the dialysis method, using ammonium sulfate as the precipitant. the crystals belong to hexagonal space group p6(2) or p6(4) with unit cell dimensions of a = b = 142.8 a and c = 63.8 a, and diffract x-rays at higher than 3.5 a resolution. a heavy-atom derivative has been prepared by soaking a crystal in an ammonium sulfate solution containing p-chloromercuriphenylsulfonate. | 1996 | 8797079 |
| cloning, expression, and sequence analysis of the three genes encoding quinoline 2-oxidoreductase, a molybdenum-containing hydroxylase from pseudomonas putida 86. | the three genes coding for quinoline 2-oxidoreductase (qor) of pseudomonas putida 86 were cloned and sequenced. the qor genes are clustered in the transcriptional order medium (m) small (s), large (l) and code for three subunits of 288 (qorm), 168 (qors), and 788 (qorl) amino acids, respectively. formation of active quinoline 2-oxidoreductase and degradation of quinoline occurred in a recombinant p. putida kt2440 clone. the amino acid sequences of qor show significant homology to various prokary ... | 1996 | 8798497 |
| the assimilation of sulfur from multiple sources and its correlation with expression of the sulfate-starvation-induced stimulon in pseudomonas putida s-313. | conditions were optimized for the batch growth of pseudomonas putida s-313 under sulfur-limited conditions. p. putida grew exponentially with sulfate as the sole source of sulfur, and growth was concomitant with the utilization of sulfate until it was exhausted. a further 20% of protein was synthesized after the apparent disappearance of sulfate. a mass balance for the utilized sulfate in cell material was calculated, given the observed molar growth yield of about 3.6 kg protein (mol s)-1 and a ... | 1996 | 8800815 |
| tetralin as a substrate for camphor (cytochrome p450) 5-monooxygenase. | camphor (cytochrome p450) 5-monooxygenase, originally isolated from the bacterium pseudomonas putida pgg 786, catalyzes the essentially stereospecific conversion of tetralin (1,2,3,4-tetrahydronaphthalene) to (r)-1-tetralol ((r).(-)-1,2,3,4-tetrahydro-1-naphthol): tetralin(aq) + nadh(aq) + o2(aq) = (r)-1-tetralol(aq) + nad(aq) + h2o(l). the ratio of the amount of (s)-1-tetralol to the amount of (r)-1-tetralol is small (approximately 0.04) and the reaction is essentially stereospecific. the react ... | 1996 | 8806731 |
| adhesion of the positively charged bacterium stenotrophomonas (xanthomonas) maltophilia 70401 to glass and teflon. | medical implants are often colonized by bacteria which may cause severe infections. the initial step in the colonization, the adhesion of bacteria to the artificial solid surface, is governed mainly by long-range van der waals and electrostatic interactions between the solid surface and the bacterial cell. while van der waals forces are generally attractive, the usually negative charge of bacteria and solid surfaces leads to electrostatic repulsion. we report here on the adhesion of a clinical i ... | 1996 | 8808938 |
| bacterial cytochromes p-450. | the cytochromes p-450 (p-450s) constitute an extremely large family ('superfamily') of haemoproteins that catalyse the oxidation of a wide range of physiological and non-physiological compounds. a remarkable feature of the p-450s is the manipulation of the same basic structure and chemistry to achieve an enormous range of functions in organisms as diverse as bacteria and man. indeed, the p-450s have been described as 'the most versatile biological catalyst known'. much research is focussed on ma ... | 1996 | 8809764 |
| the two-step lysis system of pneumococcal bacteriophage ej-1 is functional in gram-negative bacteria: triggering of the major pneumococcal autolysin in escherichia coli. | the holin function ejh of the pneumococcal bacteriophage ej-1 has been characterized. it shows structural features similar to, and functionally complemented, the prototype member of the holin family. in escherichia coli and pseudomonas putida the ejh product caused cellular death, and changes in cell morphology could be accounted for by lesions in the cytoplasmic membrane. expression of ejh resulted in the inhibition of growth in a variety of phylogenetically distant bacterial genera, suggesting ... | 1996 | 8820638 |
| involvement of sigma 54 in exponential silencing of the pseudomonas putida tol plasmid pu promoter. | the sigma 54-dependent pu promoter of the tol plasmid pww0 of pseudomonas putida becomes activated by the prokaryotic enhancer-binding xyir protein when cells encounter m-xylene in the medium. however, even in the presence of the aromatic inducer, pu activity is silenced in vivo during rapid exponential growth of the cells in rich medium. various elements known to be involved in the control of the transcriptional activity of the promoter were examined to ascertain the mechanism by which expressi ... | 1996 | 8821932 |
| characterization and expression of the plasmid-borne bedd gene from pseudomonas putida ml2, which codes for a nad+-dependent cis-benzene dihydrodiol dehydrogenase. | the catabolic plasmid phmt112 in pseudomonas putida ml2 contains the bed gene cluster encoding benzene dioxygenase (bedc1c2ba) and a nad+-dependent dehydrogenase (bedd) required to convert benzene into catechol. analysis of the nucleotide sequence upstream of the benzene dioxygenase gene cluster (bedc1c2ba) revealed a 1,098-bp open reading frame (bedd) flanked by two 42-bp direct repeats, each containing a 14-bp sequence identical to the inverted repeat of is26. in vitro translation analysis sho ... | 1996 | 8824602 |
| the nucleotide sequence of the pseudomonas aeruginosa pyre-crc-rph region and the purification of the crc gene product. | the gene (crc) responsible for catabolite repression control in pseudomonas aeruginosa has been cloned and sequenced. flanking the crc gene are genes encoding orotate phosphoribosyl transferase (pyre) and rnase ph (rph). new crc mutants were constructed by disruption of the wild-type crc gene. the crc gene encodes an open reading frame of 259 amino acids with homology to the apurinic/apyrimidinic endonuclease family of dna repair enzymes. however, crc mutants do not have a dna repair phenotype, ... | 1996 | 8824606 |
| characterization of an oprl null mutant of pseudomonas putida. | a pseudomonas putida oprl null mutant was generated with reverse genetics by using an in vitro-truncated oprl::xyle construct and in vivo allelic exchange. the nature of the mutation introduced in p. putida was confirmed by southern blotting. western blots (immunoblots) of peptidoglycan-associated proteins revealed that the oprl protein was not made in the mutant strain, whereas it was detectable as a 19-kda band in protein preparations of the wild-type strain. the p. putida oprl, mutant exhibit ... | 1996 | 8824639 |
| active efflux of toluene in a solvent-resistant bacterium. | we investigated the mechanisms behind the organic-solvent resistance of the solvent-tolerant strain pseudomonas putida s12. by use of 14c-labeled toluene, we obtained evidence that an energy-dependent export system may be responsible for this resistance to toluene. | 1996 | 8830706 |
| gram-negative bacteria can induce contact lens related acute red eye (clare) responses. | twelve volunteers participated in a study designed to measure the overnight corneal edema response with a variety of hydrogel contact lenses. during the study four subjects (5 eyes) experienced a contact lens related acute red eye (clare) reaction, which manifested as severe ocular pain, photophobia, corneal infiltration, and conjunctival hyperemia. an additional five subjects (7 eyes) developed corneal infiltrates only. twelve eyes (of 9 subjects) showed no response. | 1996 | 8835069 |
| trans unsaturated fatty acids in bacteria. | the occurrence of trans unsaturated fatty acids as by-products of fatty acid transformations carried out by the obligate anaerobic ruminal microflora has been well known for a long time. in recent years, fatty acids with trans configurations also have been detected in the membrane lipids of various aerobic bacteria. besides several psychrophilic organisms, bacteria-degrading pollutants, such as pseudomonas putida, are able to synthesize these compounds de novo. in contrast to the trans fatty aci ... | 1996 | 8835399 |
| cloning of the genes for and characterization of the early stages of toluene and o-xylene catabolism in pseudomonas stutzeri ox1. | in order to study the toluene and o-xylene catabolic genes of pseudomonas stutzeri ox1, a genomic library was constructed. a 28-kb ecori restriction endonuclease dna fragment, cloned into the vector plasmid plafr1 and designated pfb3401, permitted pseudomonas putida paw340 to convert toluene and o-xylene into the corresponding meta-ring fission products. physical and functional endonuclease restriction maps have been derived from the cloned dna fragment. further subcloning into and deletion anal ... | 1996 | 8837426 |
| amino acid sequence of catechol 1,2-dioxygenase (pyrocatechase) isozyme alpha alpha from pseudomonas arvilla c-1. | catechol 1,2-dioxygenase catalyzes the oxygenative ring cleavage of catechol to form cis,cis-muconic acid, and contains a ferric form of iron as its sole cofactor. pseudomonas arvilla c-1 contains three isozymes of the enzyme, alpha alpha, alpha beta, and beta beta [c. nakai et al. (1990) j. biol. chem. 265, 660-665]. we have determined the amino acid sequence of the alpha alpha isozyme by direct analysis. the sequence shared 77% homology with isozyme beta beta, and had conserved tyrosyl and his ... | 1996 | 8843347 |
| transcriptional regulation of the rhodococcus rhodochrous j1 nita gene encoding a nitrilase. | the 1.4-kb downstream region from a nitrilase gene (nita) of an actinomycete rhodococcus rhodochrous j1, which is industrially in use, was found to be required for the isovaleronitrile-dependent induction of nitrilase synthesis in experiments using a rhodococcus-escherichia coli shuttle vector pk4 in a rhodococcus strain. sequence analysis of the 1.4-kb region revealed the existence of an open reading frame (nitr) of 957 bp, which would encode a protein with a molecular mass of 35,100. deletion ... | 1996 | 8855219 |
| 2,4-dioxygenases catalyzing n-heterocyclic-ring cleavage and formation of carbon monoxide. purification and some properties of 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from arthrobacter sp. rü61a and comparison with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase from pseudomonas putida 33/1. | 1h-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (meqdo) was purified from quinaldine-grown arthrobacter sp. rü61a. it was enriched 59-fold in a yield of 22%, and its properties were compared with 1h-3-hydroxy-4-oxoquinoline 2,4-dioxygenase (qdo) purified from pseudomonas putida 33/1. the enzyme-catalyzed conversions were performed in an (18o)o2/(16o)o2 atmosphere. two oxygen atoms of either (18o)o2 or (16o)o2 were incorporated at c2 and c4 of the respective substrates, indicating that these unusual ... | 1996 | 8856057 |
| crystallization and preliminary x-ray crystallographic studies of ketosteroid isomerase from pseudomonas putida biotype b. | the delta(5)-3-ketosteroid isomerase from pseudomonas putida biotype b has been crystallized. the crystals belong to the space group p2(1)2(1)2(1) with unit cell dimensions of a = 36.48 angstrom, b = 74.30 angstrom, c = 96.02 angstrom, and contain one homodimer per asymmetric unit. native diffraction data to 2.19 angstrom resolution have been obtained from one crystal at room temperature indicating that the crystals are quite suitable for structure determination by multiple isomorphous replaceme ... | 1996 | 8859999 |
| cloning and characterization of the alcaligenes eutrophus 2-oxoglutarate dehydrogenase complex. | nucleotide sequence analysis of a 3.3-kb genomic ecori fragment and of relevant subfragments of a genomic 13.2-kb smai fragment of alcaligenes eutrophus, which were identified by using a dihydrolipoamide dehydrogenase-specific dna probe, revealed the structural genes of the 2-oxoglutarate dehydrogenase complex in a 7.5-kb genomic region. the genes odha (2850 bp), odhb (1248 bp), and odhl (1422 bp), encoding 2-oxoglutarate dehydrogenase (e1), dihydrolipoamide succinyltransferase (e2), and dihydro ... | 1996 | 8867378 |
| cis/trans isomerization of unsaturated fatty acids as possible control mechanism of membrane fluidity in pseudomonas putida p8. | exponentially growing cells of pseudomonas putida had an increased ratio of saturated to unsaturated fatty acids in response to increased growth temperatures. resting cells in which fatty acid biosynthesis was stopped reacted to a thermal increase by converting cis-monounsaturated fatty acids to trans isomers. cis/trans isomerization of up to 60% of the unsaturated fatty acids was also activated by alcohols of different chain length. their effective concentrations apparently depended on the lipo ... | 1996 | 8869883 |
| bacteremia due to glucose non-fermenting gram-negative bacilli in patients with hematological neoplasias and solid tumors. | twenty-six patients with hematological or solid tumors who developed bacteremia caused by stenotrophomonas maltophilia (n = 10), pseudomonas putida (n = 6), sphingomonas paucimobilis complex (n = 4) or alcaligenes xylosoxidans (n = 6) in the period between 1993 and 1995 were studied. seventeen patients were neutropenic during the infection, and 13 were undergoing bone marrow or peripheral blood stem cell transplantation. twenty-three patients had catheter-related infections; only 3 of the 26 pat ... | 1996 | 8874083 |
| accelerated biodegradation of high and low concentrations of p-nitrophenol (pnp) by bacterial inoculation in industrial wastewater: the role of inoculum size on acclimation period | the effect of inoculum size on the acclimation period and rate and extent of p-nitrophenol (pnp) degradation at high (1-10 mg/l) and low (26 &mgr;g/l) concentrations for two bacteria was determined in defined media as well as industrial wastewater. increased inoculum size did not affect the acclimation period of either bacterium at high (1-10 mg/l) pnp concentrations. at low pnp concentrations (26 &mgr;g/l), the two bacteria behaved differently. the acclimation period was shortened and both the ... | 1996 | 8875908 |
| structure and function of the pseudomonas putida integration host factor. | integration host factor (ihf) is a dna-binding and -bending protein that has been found in a number of gram-negative bacteria. here we describe the cloning, sequencing, and functional analysis of the genes coding for the two subunits of ihf from pseudomonas putida. both the ihfa and ihfb genes of p. putida code for 100-amino-acid-residue polypeptides that are 1 and 6 residues longer than the escherichia coli ihf subunits, respectively. the p. putida ihfa and ihfb genes can effectively complement ... | 1996 | 8892836 |
| the beta-ketoadipate pathway and the biology of self-identity. | the beta-ketoadipate pathway is a chromosomally encoded convergent pathway for aromatic compound degradation that is widely distributed in soil bacteria and fungi. one branch converts protocatechuate, derived from phenolic compounds including p-cresol, 4-hydroxybenzoate and numerous lignin monomers, to beta-ketoadipate. the other branch converts catechol, generated from various aromatic hydrocarbons, amino aromatics, and lignin monomers, also to beta-ketoadipate. two additional steps accomplish ... | 1996 | 8905091 |
| electron transfer in zinc-reconstituted nitrite reductase from pseudomonas aeruginosa. | 1. the catalytic cycle of the haem-containing nitrite reductase (nir) from pseudomonas aeruginosa involves electron transfer between the two prosthetic groups of the enzyme, the c-haem and the d1-haem; this reaction was shown to be slow by stopped-flow analysis. the recombinant enzyme, expressed in pseudomonas putida, contains the c-haem but no d1-haem; we have reconstituted this protein with zn-protoporphyrin ix in the place of the d1-haem. 2. photoexcitation of zn-nir is followed by electron t ... | 1996 | 8912674 |
| is1394 from pseudomonas alcaligenes n.c.i.b. 9867: identification and characterization of a member of the is30 family of insertion elements. | a new insertion sequence designated is1394 was isolated from pseudomonas alcaligenes ncib 9867 (p25x) by entrapment in plasmid pucd800 which carries the bacillus subtilis sacb and sacr genes. the 1100-bp sequence contains 27-bp inverted repeats with 4 bp mismatch and has one long open reading frame, spanning 92.1% of the entire is. the deduced 338 amino-acid sequence demonstrated homology (varying from 65% to 78% similarity and 36-67% identity) to transposases encoded by the is30 family of is el ... | 1996 | 8917085 |
| oxidation of 6,7-dihydro-5h-benzocycloheptene by bacterial strains expressing naphthalene dioxygenase, biphenyl dioxygenase, and toluene dioxygenase yields homochiral monol or cis-diol enantiomers as major products. | bacterial strains expressing naphthalene, biphenyl, and toluene dioxygenase were examined for their abilities to oxidize 6,7-dihydro-5h-benzocycloheptene (benzocyclohept-1-ene). the major oxidation products were isolated, and their absolute configurations were determined by chiral 1h nuclear magnetic resonance analysis of diastereomeric boronate esters, chiral stationary-phase high-pressure liquid chromatography, and stereo-chemical correlation. pseudomonas sp. strain 9816/11 and sphingomonas ya ... | 1996 | 8919798 |
| expression and substrate specificity of the toluene dioxygenase of pseudomonas putida ncimb 11767. | pseudomonas putida ncimb 11767 oxidized phenol, monochlorophenols, several dichlorophenols and a range of alkylbenzenes (c1-c6) via an inducible toluene dioxygenase enzyme system. biphenyl and naphthalene were also oxidized by this enzyme. growth on toluene and phenol induced the meta-ring-fission enzyme, catechol 2,3-oxygenase, whereas growth on benzoate, which did not require expression of toluene dioxygenase, induced the ortho-ring-cleavage enzyme, catechol 1,2-oxygenase. monochlorobenzoate i ... | 1996 | 8920179 |
| trichloroethylene degradation and mineralization by pseudomonads and methylosinus trichosporium ob3b. | to examine the trichloroethylene (c2hcl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of c2hcl3 mineralization were evaluated for pseudomonas cepacia g4, pseudomonas cepacia g4 pr1, pseudomonas mendocina kr1, pseudomonas putida f1, and methylosinus trichosporium ob3b using growth conditions commonly reported in the literature for expression of oxygenases responsible for c2hcl3 degradation. by varying the c2hcl3 concentration from 5 microm to 75 microm, vmax ... | 1996 | 8920197 |
| cell-free extract(s) of pseudomonas putida catalyzes the conversion of cyanides, cyanates, thiocyanates, formamide, and cyanide-containing mine waters into ammonia. | our isolate, pseudomonas putida, is known to be capable of utilizing cyanides as the sole source of carbon (c) and nitrogen (n) both in the form of free cells and cells immobilized in calcium alginate. in the present study, the cell-free extract(s) were prepared from the cells of p. putida grown in the presence of sodium cyanide. the ability of enzyme(s) to convert cyanides, cyanates, thiocyanates, formamide and cyanide-containing mine waters into ammonia (nh3) was studied at ph 7.5 and ph 9.5. ... | 1996 | 8920201 |
| characterization of type ii protein secretion (xcp) genes in the plant growth-stimulating pseudomonas putida, strain wcs358. | in pseudomonas aeruginosa, the products of the xcp genes are required for the secretion of exoproteins across the outer membrane. despite structural conservation of the xcp components, secretion of exoproteins via the xcp pathway is generally not found in heterologous organisms. to study the specificity of this protein secretion pathway, the xcp genes of another fluorescent pseudomonad, the plant growth-promoting pseudomonas putida strain wcs358, were cloned and characterized. nucleotide sequenc ... | 1996 | 8602167 |
| catabolite repression of the toluene degradation pathway in pseudomonas putida harboring pww0 under various conditions of nutrient limitation in chemostat culture. | in earlier studies, the pathway of toluene and m- and p-xylene degradation (tol pathway) in pseudomonas putida (pww0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. this report describes catabolite repression of the tol pathway by succinate in chemostat cultures run at a low dilution rate (d = 0.05 h-1) under different conditions of inorganic-nutrient limitation. the activity of benzylalcohol d ... | 1996 | 8593060 |
| cloning and characterization of styrene catabolism genes from pseudomonas fluorescens st. | a gene bank from pseudomonas fluorescens st was constructed in the broad-host-range cosmid plafr3 and mobilized into pseudomonas putida paw340. identification of recombinant cosmids containing the styrene catabolism genes was performed by screening transconjugants for growth on styrene and epoxystyrene. transposon mutagenesis and subcloning of one of the selected genome fragments have led to the identification of three enzymatic activities: a monooxygenase activity encoded by a 3-kb psti-ecori f ... | 1996 | 8572689 |
| nondisruptive detection of activity of catabolic promoters of pseudomonas putida with an antigenic surface reporter system. | a simple procedure to detect the switching on and off of catabolic promoters of pseudomonas putida, at the level of single cells based on the immunodetection of a reporter epitope expressed on the surface of bacterial cells, has been developed. to do this, the antigenic sequence asp-leu-pro-pro-asn-ser-asp-val-val-asp, from a coronavirus, was inserted genetically in the permissive site around amino acid position 153 of the lamb protein (maltose and lambda phage receptor) of escherichia coli. whe ... | 1996 | 8572699 |
| molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from comamonas testosteroni gz39. | three strains of comamonas testosteroni were isolated from river sediment for the ability to degrade phenanthrene; two of the strains also grew on naphthalene, and one strain also grew on anthracene. the homology of the genes for polycyclic aromatic hydrocarbon degradation in these strains to the classical genes (nah) for naphthalene degradation from pseudomonas putida ncib 9816-4 was determined. the three c. testosteroni strains showed no homology to the nah gene probe even under low-stringency ... | 1996 | 8572701 |
| manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2. | a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ... | 1996 | 8555170 |
| nucleotide sequence of salicylate hydroxylase gene and its 5'-flanking region of pseudomonas putida kf715. | the salicylate hydroxylase, a flavoprotein monooxygenase, catalyzes the decarboxylative hydroxylation of salicylate to form catechol. nucleotide sequence of a salicylate hydroxylase gene and its 5'-flanking region in chromosomal dna of pseudomonas putida kf715 was analyzed. the salicylate hydroxylase was encoded in an open reading frame with 1308 base pairs which can encode a polypeptide of molecular weight 48 kda with 435 amino acids. the open reading frame was preceded by a putative ribosome-b ... | 1996 | 8561793 |
| pcr detection of metallo-beta-lactamase gene (blaimp) in gram-negative rods resistant to broad-spectrum beta-lactams. | we applied pcr to the rapid detection of the metallo-beta-lactamase gene, blaimp, in clinically isolated gram-negative rods. a total of 54 high-level ceftazidime-resistant strains (mics, > 128 micrograms/ml) were subjected to pcr analyses with the blaimp-specific primers, since the blaimp-bearing clinical isolates tested in our previous study always demonstrated high-level resistance to ceftazidime. twenty-two blaimp-positive strains including 9 pseudomonas aeruginosa, 9 serratia marcescens, 2 a ... | 1996 | 8940421 |
| the amino acid sequence of rat kidney 5-oxo-l-prolinase determined by cdna cloning. | 5-oxoprolinase (ec 3.5.2) catalyzes a reaction in which the endergonic cleavage of 5-oxo-l-proline to form l-glutamate is coupled to the exergonic hydrolysis of atp to adp and inorganic phosphate. highly purified preparations of the enzyme have been obtained from rat kidney and pseudomonas putida. the rat kidney enzyme is composed of two strongly interacting, apparently identical subunits (mr = 142,000), whereas that from p. putida is composed of two functionally different protein components tha ... | 1996 | 8943290 |
| analysis of cumene (isopropylbenzene) degradation genes from pseudomonas fluorescens ip01. | we obtained the dna fragments encoding 2-hydroxy-6-oxo-7-methylocta-2,4-dienoic acid (homoda) hydrolase in the cumene (isopropylbenzene) degrader pseudomonas fluorescens strain ip01 via pcr using two synthesized oligonucleotides corresponding to the conserved regions within known meta-cleavage compound hydrolases. following colony hybridization using the amplified dna as a probe, a 4.5-kb hindiii fragment was isolated from p. fluorescens ip01. after determining the nucleotide sequence of this fr ... | 1996 | 8953719 |
| activity and three-dimensional distribution of toluene-degrading pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy. | as a representative member of the toluene-degrading population in a biofilter for waste gas treatment, pseudomonas putida was investigated with a 16s rrna targeting probe. the three-dimensional distribution of p. putida was visualized in the biofilm matrix by scanning confocal laser microscopy, demonstrating that p. putida was present throughout the biofilm. acridine orange staining revealed a very heterogeneous structure of the fully hydrated biofilm, with cell-free channels extending from the ... | 1996 | 8953734 |
| characterization of a 7fe ferredoxin isolated from the marine denitrifier pseudomonas nautica strain 617: spectroscopic and electrochemical studies. | a 7fe ferredoxin, isolated from the marine denitrifier pseudomonas nautica strain 617, was characterized. the nh2-terminal sequence analysis, performed until residue number 56, shows a high similarity with the 7fe ferredoxins isolated from azotobacter vinelandii, pseudomonas putida, and pseudomonas stutzeri. epr and nmr spectroscopies identify the presence of both [3fe-4s] and [4fe-4s] clusters, with cysteinyl coordination. the electrochemical studies on [fe-s] clusters show that a fast diffusio ... | 1996 | 8954931 |
| mannitol, a novel bacterial compatible solute in pseudomonas putida s12. | the aim of this study was to identify the compatible solutes accumulated by pseudomonas putida s12 subjected to osmotic stress. in response to reduced water activity, p. putida s12 accumulated nalpha-acetylglutaminylglutamine amide (naggn) simultaneously with a novel compatible solute identified as mannitol (using 13c- and 1h-nuclear magnetic resonance, liquid chromatography-mass spectroscopy and high-performance liquid chromatography methods) to maximum concentrations of 74 and 258 micromol g ( ... | 1996 | 8955280 |
| identification and characterization of the tolqra genes of pseudomonas aeruginosa. | the tolq, tolr, and tola genes from pseudomonas aeruginosa pao were cloned using degenerate oligonucleotide pcr primers designed based on conserved transmembrane regions of escherichia coli tolq and tolr and e. coli and pseudomonas putida exbb and exbd. the resulting pcr product was used as a probe to isolate a 6.5-kb dna fragment containing p. aeruginosa tolq, tolr, and tola. the nucleotide sequence of a 2.9-kb dna fragment containing the tolq, tolr, and tola genes was determined. the dna seque ... | 1996 | 8955385 |
| clinical spectrum of pseudomonas putida infection. | the clinical and microbiologic characteristics of 55 cases of pseudomonas putida infection in 53 patients in a medical center in taiwan from april 1988 to march 1993 are reported. p. putida was cultured in the decreasing order of frequency from urine (24 isolates), sputum (12), blood (10), wound discharge (3), peritoneal fluid (3), cerebrospinal fluid (2) and umbilical swab (1). of the adult patients, 23% (12/53) were considered to be contaminated or colonized with p. putida. of the 41 patients ... | 1996 | 8961672 |
| the effect of nutrient limitation on styrene metabolism in pseudomonas putida ca-3. | styrene degradation in pseudomonas putida ca-3 has previously been shown to be subject to catabolite repression in batch culture. we report here on the catabolite-repressing effects of succinate and glutamate and the effects of a limiting inorganic-nutrient concentration on the styrene degradation pathway of p. putida ca-3 in a chemostat culture at low growth rates (0.05 h-1). oxidation of styrene and the presence of styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities were us ... | 1996 | 8967774 |
| molecular cloning and expression in different microbes of the dna encoding pseudomonas putida u phenylacetyl-coa ligase. use of this gene to improve the rate of benzylpenicillin biosynthesis in penicillium chrysogenum. | the gene encoding phenylacetyl-coa ligase (pcl), the first enzyme of the pathway involved in the aerobic catabolism of phenylacetic acid in pseudomonas putida u, has been cloned, sequenced, and expressed in two different microbes. in both, the primary structure of the protein was studied, and after genetic manipulation, different recombinant proteins were analyzed. the pcl gene, which was isolated from p. putida u by mutagenesis with the transposon tn5, encodes a 48-kda protein corresponding to ... | 1996 | 8969218 |
| comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. | the degradation of trichloroethylene (tce) by toluene-oxidizing bacteria has been extensively studied, and yet the influence of environmental conditions and physiological characteristics of individual strains has received little attention. to consider these effects, the levels of tce degradation by strains distinguishable on the basis of toluene and nitrate metabolism were compared under aerobic or hypoxic conditions in the presence and absence of nitrate and an exogenous electron donor, lactate ... | 1996 | 8975612 |
| organisation of the tmb catabolic operons of pseudomonas putida tmb and evolutionary relationship with the xyl operons of the tol plasmid pww0. | in pseudomonas putida (pp) tmb the genes involved in the catabolism of methyl-substituted aromatic hydrocarbons 1,2,4-trimethylbenzene, m- and p-xylene (tmb operon), are functionally and genetically homologous to the xyl genes of the plasmid pww0, but are chromosomally encoded. we have analysed by cloning. southern blotting and sequencing of selected regions the organisation of the tmb cluster. this analysis shows that the structural and regulatory genes of the tmb and xyl systems exhibit a high ... | 1996 | 8982087 |
| cotranscription of a gtpase gene from the cyanobacterium synechocystis pcc 6803 and a p-type ca(2+)-atpase gene. | a gtpase gene adjacent to the ca(2+)-atpase gene from synechocystis pcc 6803 has been sequenced. it encodes for a protein of 456 amino acids revealing high homology to so-called 50k proteins of bacillus subtilis and pseudomonas putida. cotranscription of gtpase and ca(2+)-atpase genes has been shown by reverse transcription pcr. | 1996 | 8982253 |
| biodegradation of hydrogen sulfide by a laboratory-scale immobilized pseudomonas putida ch11 biofilter. | a heterotrophic pseudomonas putida ch11 was isolated from livestock farming wastewater and applied for the treatment of h2s-containing gas. extensive tests including removal characteristics, metabolic products, and removal efficiencies of h2s by p. putida ch11 were examined in batch and continuous systems. the optimum ph required to remove hydrogen sulfide was found in the range of 6-8. the maximum removal rate and the saturation constant were calculated to be vm = 1.36 g s/day.kg dry bead and k ... | 1996 | 8983205 |
| luciferase-dependent, cytochrome p-450-catalyzed dehalogenation in genetically engineered pseudomonas. | to investigate the possibility of luciferase-dependent photoreduction of cytochrome p-450's in vivo, vibrio harveyi luciferase was coexpressed with the bacterial cytochrome p-450cam in pseudomonas putida. luciferase expression was under the control of the pm promoter from the meta-cleavage tol operon, incorporated into the chromosome by a mini tn5-mediated transposition. cytochrome p-450cam expression was controlled by the ptac-lac promoter on the broad host range vector pmmb206. both proteins w ... | 1996 | 8987475 |
| construction and use of recombinant escherichia coli strains for the synthesis of toluene cis-glycol. | the toluene dioxygenase genes derived from pseudomonas putida ncimb 11767 were subcloned from a previously constructed recombinant plasmid, pig, using puc18 as the cloning vector and e. coli tg2 as the host strain. the resulting strain, e. coli tg2 (p1/1), produced toluene cis-glycol when grown in lb broth or minimal medium in the presence of toluene. restriction mapping and partial dna sequencing provided evidence for the presence of orfs with extensive homology to parts of the tod operon from ... | 1996 | 8987488 |
| cometabolic degradation of 4-chlorophenol by alcaligenes eutrophus. | alcaligenes eutrophus was grown in batch cultures using either phenol as a sole substrate or mixtures of phenol and 4-chlorophenol. phenol was found to be the sole source for carbon and energy while 4-chlorophenol was utilized only as a cometabolite. maximum growth rates on phenol reached only 0.26 h-1, significantly below the growth rates reported earlier with pseudomonas putida. the cometabolite was found to decrease biomass yield and increase lag time before logarithmic growth occurred. both ... | 1996 | 8987646 |
| degradation of 3-nitrophenol by pseudomonas putida b2 occurs via 1,2,4-benzenetriol. | growth of pseudomonas putida b2 in chemostat cultures on a mixture of 3-nitrophenol and glucose induced 3-nitrophenol and 1,2,4-benzenetriol-dependent oxygen uptake activities. anaerobic incubations of cell suspensions with 3-nitrophenol resulted in complete conversions of the substrate to ammonia and 1,2,4-benzenetriol. this indicates that p. putida b2 degrades 3-nitrophenol via 1,2,4-benzenetriol, via a pathway involving a hydroxylaminolyase. involvement of this pathway in nitroaromatic metabo ... | 1996 | 8987889 |
| 3-hydroxyisobutyrate dehydrogenase from pseudomonas putida e23: purification and characterization. | the nad(+)-dependent 3-hydroxyisobutyrate dehydrogenase [ec 1.1.1.31] was purified to homogeneity from pseudomonas putida e23. the enzyme was a tetramer (molecular mass, 120 kda) consisted of identical subunits (molecular mass, 30 kda). the enzyme was specific for nad+ (km, 0.44 mm). the maximal activity was obtained at about ph 10. the enzyme was specific for the l-isomer of 3-hydroxyisobutyrate. in addition to l-3-hydroxyisobutyrate, l-serine, 2-methyl-dl-serine, and 3-hydroxypropionate were s ... | 1996 | 8988636 |
| anticancer efficacy of methioninase in vivo. | therapeutics that are selective for cancer would have a high potential for efficacy. we have previously shown that the metabolic defect of enhanced methionine dependence is a broad cancer-selective target. methionine depletion can completely arrest the growth of methionine-dependent tumor cells in vivo with a reversible pre-mitosis cell-cycle block. dietary methionine depletion can partially arrest the growth of methionine-dependent rodent tumors in vivo. this report demonstrates that methionina ... | 1996 | 9042315 |
| serum methionine depletion without side effects by methioninase in metastatic breast cancer patients. | the growth dependence of human tumors on elevated levels of methionine has been shown in preclinical in vitro and in vivo studies to be a frequently occurring, highly effective, tumor-selective therapeutic target. high purity endotoxin-free methioninase was produced from pseudomonas putida in order to develop anti-methionine chemotherapy targeting of human tumors. a pilot phase i clinical trial has been initiated in order to determine methioninase toxicity, the pharmacokinetics of methioninase a ... | 1996 | 9042316 |
| synthesis of fragments of the peptide component of pseudobactin. | pseudobactin is a structurally complex and physiologically important siderophore (microbial iron chelator] from pseudomonas putida-fluorescens. various fragments of the unusual peptide component of pseudobactin listed below were prepared by solution-phase peptide synthesis. l-lys.d-threo-beta-oh asp.l-ala.d-allo-thr.l-ala l-lys.d-threo-beta oh asp.l-ala.d-allo-thr d-threo-beta-oh asp.l-ala.d-allo-thr.l-ala.d-n-oh-cycloorn d-threo-beta-oh-asp.l-ala.d-allo-thr.l-ala l-ala.d-allo-thr.l-ala.d-n-oh-c ... | 1996 | 9231324 |
| molecular characterization of the 4-hydroxyphenylacetate catabolic pathway of escherichia coli w: engineering a mobile aromatic degradative cluster. | we have determined and analyzed the nucleic acid sequence of a 14,855-bp region that contains the complete gene cluster encoding the 4-hydroxyphenylacetic acid (4-hpa) degradative pathway of escherichia coli w (atcc 11105). this catabolic pathway is composed by 11 genes, i.e., 8 enzyme-encoding genes distributed in two putative operons, hpabc (4-hpa hydroxylase operon) and hpagedfhi (meta-cleavage operon); 2 regulatory genes, hpar and hpaa; and the gene, hpax, that encodes a protein related to t ... | 1996 | 8550403 |
| initial steps in the degradation of 3,4-dimethylbenzoic acid by pseudomonas putida strain dmb. | pseudomonas putida strain dmb capable of growing on 3,4-dimethylbenzoic acid as the only c and energy source was isolated by enrichment techniques. it does not utilize for growth or cooxidize the other dimethylbenzoate isomers tested. 3,4-dimethylsalicylic acid, 3,4-dimethylphenol and 3,4-dimethylcatechol were isolated and identified by nuclear magnetic resonance and mass spectra in the reaction mixture of p. putida washed cells. the detection of the two first metabolites suggests that the initi ... | 1996 | 8998974 |
| outer membrane protein heterogeneity within pseudomonas fluorescens and p. putida and use of an oprf antibody as a probe for rrna homology group i pseudomonads. | the electrophoretic patterns of outer membrane proteins of strains representing the biovars of pseudomonas fluorescens and pseudomonas putida were analyzed by gel electrophoresis. the outer membrane protein profiles were variable, and they were not useful for assigning strains to a specific biovar. however, three or four predominant outer membrane proteins migrating at 42 to 46 kda, 33 to 38 kda, and 20 to 22 kda were conserved among the strains. they could be tentatively identified as opre (44 ... | 1996 | 16535235 |
| biosynthesis of novel aromatic copolyesters from insoluble 11-phenoxyundecanoic acid by pseudomonas putida bm01. | two types of novel aromatic copolyesters were synthesized from 11-phenoxyundecanoic acid (11-pou) as the sole carbon source and the cosubstrates 11-pou and octanoate, respectively, by isolated pseudomonas putida bm01 that is known to accumulate high concentrations of medium-chain-length polyesters. insoluble 11-pou was recrystallized in situ in buffer by alkaline treatment and ph adjustment, followed by autoclaving. the resulting microcrystals, whose structure was different from that of the comm ... | 1996 | 16535240 |
| cell envelope changes in solvent-tolerant and solvent-sensitive pseudomonas putida strains following exposure to o-xylene. | solvent-tolerant and -sensitive pseudomonas putida strains were studied to determine their cell envelope changes following exposure to o-xylene. both strains produced trans-unsaturated fatty acids. the tolerant strain showed an increase in total fatty acids, an increase in saturated fatty acids, and modified lipopolysaccharide. it is suggested that these envelope modifications aid in survival at high concentrations of organic solvents. | 1996 | 16535264 |
| biotransformation of 6,6-dimethylfulvene by pseudomonas putida re213. | the biotransformation of 6,6-dimethylfulvene [5-(1-methylethylidene)-1,3-cyclopentadiene], a nonaromatic c(inf5) carbocyclic analog of isopropylbenzene, was examined by using pseudomonas putida re213, a tn5-generated dihydrodiol-accumulating mutant of the isopropylbenzene-degrading strain p. putida re204. 6,6-dimethylfulvene was converted to a single chiral product identified as (+)-(1r,2s)-cis-1,2-dihydroxy-5-(1-methylethylidene)-3-cyclopentene. this isopropylbenzene 2,3-dioxygenase-catalyzed t ... | 1996 | 16535266 |
| use of an ipb-lux fusion to study regulation of the isopropylbenzene catabolism operon of pseudomonas putida re204 and to detect hydrophobic pollutants in the environment. | a dna segment involved in the regulation of the isopropylbenzene (cumene) catabolism operon (ipb) of plasmid pre4 from pseudomonas putida re204 and the vibrio fischeri luciferase genes, luxcdabe, were used to create an ipbro/pa(prm1)-luxcdabe reporter fusion plasmid, pos25. escherichia coli hms174(pos25) produces light in the presence of inducers of the ipb operon. these inducers were shown to be hydrophobic compounds and to include monoalkylbenzenes, substituted benzenes and toluenes, some alka ... | 1996 | 16535269 |
| use of colicin e3 for biological containment of microorganisms. | the genetic determinant of the lethal antibiotic colicin e3 was cloned under the control of a tightly regulated promoter in the absence of the gene for its cognate inhibitor. combination of this killing cassette with a stringent regulatory element provided a substrate-dependent conditional suicide system that was exploited for the biological containment of a pseudomonas putida strain. the lethality of a single gene copy and the distinct and universal cellular target of the antibiotic suggest col ... | 1996 | 16535323 |
| effect of environmental factors on the trans/cis ratio of unsaturated fatty acids in pseudomonas putida s12. | the membrane reactions of pseudomonas putida s12 to environmental stress were investigated. cells reacted to the addition of six different heavy metals with an increase in the ratio of trans to cis unsaturated fatty acids. a correlation among the increase in the trans/cis ratio, the toxic effects of the heavy metals, and nonspecific permeabilization of the cytoplasmic membrane, as indicated by an efflux of potassium ions, was measured. cells previously adapted to toxic concentrations of toluene ... | 1996 | 16535373 |
| o-alkyl hydroxamates as metaphors of enzyme-bound enolate intermediates in hydroxy acid dehydrogenases. inhibitors of isopropylmalate dehydrogenase, isocitrate dehydrogenase, and tartrate dehydrogenase(1). | the inhibition of thermus thermophilus isopropylmalate dehydrogenase by o-methyl oxalohydroxamate was studied for comparison to earlier results of schloss with the salmonella enzyme. it is a fairly potent (1.2 &mgr;m), slow-binding, uncompetitive inhibitor against isopropylmalate and is far superior to an oxamide (25 mm k(i) competitive) that is isosteric with the ketoisocaproate product of the enzyme. this improvement in inhibition was attributed to its increased nh acidity, which presumably is ... | 1996 | 11667376 |
| biodegradation of 3-chlorobenzoate by pseudomonas putida 10.2. | pseudomonas putida 10.2, a 3-chlorobenzoate (3cba)-degrading bacterium, was isolated from a soil sample obtained from an agricultural area in chiang mai, thailand. this bacterium could degrade 2mm 3cba very rapidly with the concomitant formation of chloride ion when grown in mineral salt-yeast extract medium. the presence of glucose, lactose and pyruvate in the medium reduced the capability of this bacterium to degrade 3cba. metabolites such as 3-chlorocatechol (3cc), catechol and cis,cis-muconi ... | 1996 | 24415423 |
| removal of caffeine in sewage by pseudomonas putida: implications for water pollution index. | a strain of pseudomonas putida (biotype a) capable of growing on caffeine (1,3,7-trimethylxanthine) was isolated from a domestic wastewater processing operation. it used caffeine as the sole carbon source with a mean growth rate constant (k) of 0.049 h(-1) (approximately 20 h per generation), whereas k for glucose utilization under similar incubation conditions was 0.31 (3.3 h per generation). the isolate contained at least two plasmids, and the increased expression of a 40 kda protein was attri ... | 1996 | 24415233 |
| short communication: isolation of a bacterium capable of limited degradation of industrial and labelled, natural and synthetic lignins. | pseudomonas putida, isolated from decomposing plant materials, degraded several lignin-related aromatic compounds. after 30 days of incubation in media containing polymeric kraft-lignin (pkl), the amount of klason lignin had decreased by about 13%. when (14)c-labelled dehydropolymers of coniferyl alcohol (dhp) lignins and (14)c-lignin-lignocelluloses were used as substrates, mineralization to (14)co2 by the p. putida strain ranged from 1.4% to 2.1%. | 1996 | 24415105 |
| effect of endogenous methylglyoxal on chinese hamster ovary cells grown in culture. | methylglyoxal is a ketoaldehyde that reacts readily under physiological conditions with biologically relevant ligands, such as amine and sulfhydryl groups. it is produced in mammalian cells primarily as a by-product of glycolysis. the level of glucose, l-glutamine and fetal bovine serum in culture media was found to significantly affect levels of intracellular methylglyoxal in chinese hamster ovary cells. medium with 25 mm glucose and 5 mm l-glutamine caused an increase in free methylglyoxal lev ... | 1996 | 22358913 |
| plants--rhizospheric organisms interaction in a manmade system with and without biogenous element limitation. | the effect has been studied of inoculation of seeds of wheat with two species of rhizospheric microorganisms--pseudomonas fluorescens and pseudomonas putida--on young plant growth with complete and with nitrogen deficit mineral nutrition. with complete mineral medium, plants grown from seeds inoculated with bacteria of pseudomonas genus (experiment plants) have been found to have better growth over plants not inoculated with these bacteria (control plants). the experiment plants had increased tr ... | 1997 | 11542573 |
| crystallization and preliminary diffraction studies of morphinone reductase, a flavoprotein involved in the degradation of morphine alkaloids. | morphinone reductase from pseudomonas putida m10, a flavoprotein involved in the degradation of morphine alkaloids, was purified from an overexpressing strain of escherichia coli and crystallized using the hanging-drop vapour-diffusion method. diffraction data were collected to 2.5 a. the i-centred orthorhombic cell has a monomer in the asymmetric unit. preliminary molecular replacement calculations have been performed using old yellow enzyme as the search model. | 1997 | 15299897 |
| characterization of two crystal forms of 3-carboxy-cis,cis-muconate lactonizing enzyme from pseudomonas putida. | two crystal forms of 3-carboxy-cis,cis-muconate lactonizing enzyme from pseudomonas putida have been characterized. form a is in space group p6, with unit-cell dimensions a = b = 232, c = 79 a, alpha = beta = 90, gamma = 120 degrees. form b is orthorhombic, with cell dimensions a = 163, b = 139, c = 90 a alpha = beta = gamma = 90 degrees. | 1997 | 15299946 |
| metabolism of naphthalene, 1-naphthol, indene, and indole by rhodococcus sp. strain ncimb 12038. | the regulation of naphthalene and 1-naphthol metabolism in a rhodococcus sp. (ncimb 12038) has been investigated. the microorganism utilizes separate pathways for the degradation of these compounds, and they are regulated independently. naphthalene metabolism was inducible, but not by salicylate, and 1-naphthol metabolism, although constitutive, was also repressed during growth on salicylate. the biochemistry of naphthalene degradation in this strain was otherwise identical to that found in pseu ... | 1997 | 16535479 |
| toluene elicits a carbon starvation response in pseudomonas putida mt-2 containing the tol plasmid pww0. | pseudomonas putida mt-2(pwwo) exhibited a carbon starvation response in the presence of toluene, a utilizable carbon source. when growth-supporting (4-mg/liter), inhibitory (130-mg/liter), and lethal (267-mg/ liter) levels of toluene were provided as the sole carbon source, p. putida responded by rapidly inhibiting protein synthesis and by producing 26 new proteins, 22 of which overlapped with those induced by carbon starvation. p. putida produced the same proteins when cultures were starved by ... | 1997 | 16535605 |
| metabolism of chlorotoluenes by burkholderia sp. strain ps12 and toluene dioxygenase of pseudomonas putida f1: evidence for monooxygenation by toluene and chlorobenzene dioxygenases. | the degradation of toluene by pseudomonas putida f1 and of chlorobenzenes by burkholderia sp. strain ps12 is initiated by incorporation of dioxygen into the aromatic nucleus to form cis-dihydrodihydroxybenzenes. toluene-grown cells of p. putida f1 and 3-chlorobenzoate-grown cells of burkholderia sp. strain ps12 were found to monooxygenate the side chain of 2- and 3-chlorotoluene to the corresponding chlorobenzyl alcohols. further metabolism of these products was slow, and the corresponding chlor ... | 1997 | 16535607 |
| manure enhances plasmid mobilization and survival of pseudomonas putida introduced into field soil. | we report a field study on plasmid mobilization in an agricultural soil. the influence of pig manure on the mobilization of the incq plasmid pie723 by indigenous plasmids or by the incp(alpha) plasmid pgp527 into the recipient pseudomonas putida uwc1 (rif(supr) nal(supr)) was studied in field soil. six plots were prepared in duplicate, three of which were treated with manure prior to inoculation of the donor and recipient strains. as a donor strain, either escherichia coli j53(pie723) or e. coli ... | 1997 | 16535608 |
| in situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes. | reverse transcription of rna molecules inside intact bacterial cells was carried out by using reverse transcriptase with a single oligonucleotide complementary to specific 16s rrna or mrna sequences. fluorescently labeled nucleotides were incorporated into each transcribed cdna inside cells. this protocol is termed in situ reverse transcription (isrt). in this study, by using species-specific primers targeting unique regions of the 16s rrna sequences, isrt was used successfully to detect and enu ... | 1997 | 16535753 |
| enhancement of biodegradation of phenol and a nongrowth substrate 4-chlorophenol by medium augmentation with conventional carbon sources. | the enhancement of biodegradation of phenol and 4-chlorophenol (4-cp) as a cometabolised compound by pseudomonas putida atcc 49451 was accomplished by augmenting the medium with conventional carbon sources such as sodium glutamate and glucose. compared with phenol as the sole carbon source, the addition of 1 gl(-1) sodium glutamate increased the toxicity tolerance of cells toward 4-cp and significantly improved the biodegradation rates of both phenol and 4-cp even when the initial concentration ... | 1997 | 15765612 |
| relative expression and stability of a chromosomally integrated and plasmid-borne marker gene fusion in environmentally competent bacteria. | a xyle-icec transcriptional fusion was created by ligatinga dna fragment harboring the cloned xyle structural gene from the tol plasmid of pseudomonas putida mt-2 into the cloned icec gene of pseudomonas syringae cit7. this fusion construct was integrated into the chromosome of pseudomonas syringae cit7 by homologous recombination. both cis-merodiploid strain cit7m17 and marker exchange strain cit7h69 produced the xyle gene product, catechol2,3-dioxygenase. strain cit7m17, in which xyle was infl ... | 1997 | 9003582 |
| neisseria meningitidis tonb, exbb, and exbd genes: ton-dependent utilization of protein-bound iron in neisseriae. | we have recently cloned and characterized the hemoglobin (hb) receptor gene, hmbr, from neisseria meningitidis. to identify additional proteins that are involved in hb utilization, the n. meningitidis hb utilization system was reconstituted in escherichia coli. five cosmids from n. meningitidis dna library enabled a heme-requiring (hema), hmbr-expressing mutant of e. coli to use hb as both porphyrin and iron source. nucleotide sequence analysis of dna fragments subcloned from the hb-complementin ... | 1997 | 9006036 |
| recombinant methioninase infusion reduces the biochemical endpoint of serum methionine with minimal toxicity in high-stage cancer patients. | the tumor-specific increased minimal requirement for methionine has been shown to be a highly promising therapeutic target. to attack this target we have previously cloned the methioninase gene from pseudomonas putida and produced recombinant methioninase (rmetase). a pilot phase i clinical trial has been carried out to determine rmetase toxicity, rmetase pharmacokinetics, and serum met-depletion in cancer patients. patients with advanced breast cancer, lung cancer, renal cancer and lymphoma wer ... | 1997 | 9427792 |
| in vitro activities of granule-bound poly[(r)-3-hydroxyalkanoate]polymerase c1 of pseudomonas oleovorans--development of an activity test for medium-chain-length-poly(3-hydroxyalkanoate) polymerases. | a newly developed in vitro activity assay for medium-chain-length (mcl)-poly(3-hydroxyalkanoate) polymerases is described. polymerase c1 of pseudomonas oleovorans gpo1 attached to isolated granules was used as model enzyme. a direct correlation was found between (r)-3-hydroxyoctanoylcoa depletion and poly(3-hydroxyalkanoate) synthesis due to polymerase c1 activity. highest activities of 1.13 u/mg granule bound protein and highest specific activities of 2.3 u/mg polymerase c1 were determined towa ... | 1997 | 9428695 |
| principal transcription sigma factors of pseudomonas putida strains mt-2 and g1 are significantly different. | the rpod gene coding for the primary transcription sigma factor, sigma70, and its entire operon were cloned from strain mt-2 of the purple soil bacterium pseudomonas putida. comparison of the deduced amino acid sequence of ppmt-2 sigma70 with that of sigma70 from p. putida strain g1 shows that the two proteins differ in their primary structure, molecular weight, and isoelectric point. the significance of this difference is discussed in terms of bacterial taxonomy and transcription regulation. | 1997 | 9434175 |
| degradation of nonionic surfactants and polychlorinated biphenyls by recombinant field application vectors. | degradation of polychlorinated biphenyls (pcbs) in the environment is limited by their aqueous solubility and the degradative competence of indigenous populations. field application vectors (favs) have been developed in which surfactants are used to both increase the solubility of the pcbs and support the growth of surfactant-degrading strains engineered for pcb degradation. surfactant and pcb degradation by two recombinant strains were investigated. pseudomonas putida ipl5 utilizes both alkylet ... | 1997 | 9439001 |
| cloning and sequence analysis of a catechol 2,3-dioxygenase gene from the nitrobenzene-degrading strain comamonas sp js765. | comamonas sp strain js765 utilizes nitrobenzene as a carbon and nitrogen source. the initial attack on nitrobenzene is carried out by nitrobenzene 1,2-dioxygenase, which converts nitrobenzene to an unstable nitrohydrodiol that spontaneously decomposes to form catechol and nitrite. catechol is then degraded via a meta cleavage pathway. we now report the cloning of a dna fragment carrying a catechol 2,3-dioxygenase gene from js765. nucleotide sequence analysis revealed three open reading frames (o ... | 1997 | 9451836 |
| effect of long-term depletion of plasma methionine on the growth and survival of human brain tumor xenografts in athymic mice. | depletion of plasma methionine is expected to inhibit or reverse growth of methionine-dependent tumors; however, modulation of methionine and other sulfur amino acids is not a trivial task in experimental animals. l-methioninase from pseudomonas putida at 1,000 u/kg causes acute reduction of plasma methionine by 80% in mice, but recovery occurs within 14 hours. restriction of dietary choline and replacement of dietary methionine with homocystine results in 50% chronic reduction of plasma methion ... | 1997 | 9457739 |
| over-production of stereoselective nitrile hydratase from pseudomonas putida 5b in escherichia coli: activity requires a novel downstream protein. | the stereoselective nitrile hydratase (nhase) from pseudomonas putida 5b has been over-produced in escherichia coli. maximal enzyme activity requires the co-expression of a novel downstream gene encoding a protein (p14k) of 127 amino acids, which shows no significant homology to any sequences in the protein database. nitrile hydratase produced in transformed e. coli showed activity as high as 472 units/mg dry cell (sixfold higher than 5b), and retained the stereoselectivity observed in the nativ ... | 1997 | 9457799 |
| identification of major antigenic proteins of pasteurella piscicida. | two different antigenic protein-coding clones (ppa1 and ppa2) were isolated using anti-pasteurella piscicida rabbit serum from a genomic dna library of p. piscicida strain kp9038. the ppa1 and ppa2 expressed 7 kda and 45 kda proteins in escherichia coli, respectively, and the molecular sizes of these expressed proteins are the same as these of the major antigenic proteins of p. piscicida. ppa1 encodes a protein of 83 amino acids residues, which is similar to the bacterial lipoprotein. comparison ... | 1997 | 9441863 |
| isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine. | bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ... | 1997 | 10932719 |
| contamination of diagnostic ophthalmic solutions in primary eye care settings. | pharmaceutical agents and irrigating solutions are widely used in both optometric and ophthalmologic practices. contamination of these containers or solutions could possibly pose some risk of infection to a patient. we set out to investigate the possible contamination of a representative sample of these containers in small office practices. representative bottles of two diagnostic pharmaceutical agents and an irrigating solution were obtained from primary care optometric and ophthalmologic pract ... | 1997 | 9232983 |
| cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of aeromonas caviae. | a 5.0-kbp ecorv-ecori restriction fragment was cloned and analyzed from genomic dna of aeromonas caviae, a bacterium producing a copolyester of (r)-3-hydroxybutyrate (3hb) and (r)-3-hydroxyhexanoate (3hhx) [p(3hb-co-3hhx)] from alkanoic acids or oils. the nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (pha) synthase gene (phac(ac) [i.e., the phac gene from a. caviae]) together with four open reading frames (orf1, -3, -4, and -5) and one putative promoter region. t ... | 1997 | 9244271 |
| importance of biofilm formation for corrosion inhibition of sae 1018 steel by axenic aerobic biofilms. | to investigate if corrosion inhibition by aerobic biofilms is a general phenomenon, carbon steel (sae 1018) coupons were exposed to a complex liquid medium (luria-bertani) and seawater-mimicking medium (vnss) containing fifteen different pure-culture bacterial suspensions representing seven genera. compared to sterile controls, the mass loss in the presence of these bacteria (which are capable of developing a biofilm to various degrees) decreased by 2- to 15-fold. the extent of corrosion inhibit ... | 1997 | 9248069 |
| physiological stress in batch cultures of pseudomonas putida 54g during toluene degradation. | physiological stress associated with toluene exposure in batch cultures of pseudomonas putida 54g was investigated. p. putida 54g cells were grown using a continuous vapor phase feed stream containing 150 ppmv or 750 ppmv toluene as the sole carbon and energy source. cells were enumerated on non-selective (r2a agar plates) and a selective minimal medium incubated in the presence of vapor phase toluene (hcmm2). differential recovery on the two media was used to evaluate bacterial stress, culturab ... | 1997 | 9248070 |