Publications

protection of germ-free mice from infection by helicobacter felis after active oral or passive iga immunization.	helicobacter pylori infection of human gastric epithelium has been associated with gastritis, ulcers and gastric cancers. in an h. felis, germ-free mouse model of infection, oral immunization with bacterial antigens plus cholera toxin resulted in elevated serum, gastric and intestinal anti-h. felis antibody titres and protection from acute infection. mice given monoclonal iga anti-h. felis antibody at the time of initial challenge were also protected from infection. these results demonstrate tha ...	1993	8322486
helicobacter pylori: a cause of chronic abdominal pain in children.	approximately one in ten children are seen by a health care professional for a complaint of chronic recurrent abdominal pain. in most cases these complaints are non-organic. organic causes of abdominal pain may include gastroesophageal reflux, peptic ulcer disease, gallbladder, pancreatic or kidney disease. in addition, infection of the gastric antrum with helicobacter pylori is now included in the differential diagnosis of chronic abdominal pain in children. h. pylori are "s" or "c" shaped, gra ...	1993	8323993
rapid test for detection of antibodies to helicobacter pylori.		1993	8324158
the inflammatory response of the gastric mucosa of mice experimentally infected with "gastrospirillum suis".	to establish an experimental model to study gastric spiral non-cultivable bacteria, 30 4-week-old female cfw (lob) mice were inoculated with porcine gastric mucus containing "gastrospirillum suis" and 25 mice were inoculated with mucus without "g. suis". mice were examined 3, 7, 14, 21, 28 and 60 days after inoculation. fragments from the membranous, oxyntic and antral gastric mucosa and from the duodenal mucosa were obtained for histological and microbiological analysis. tightly spiralled bacte ...	1993	8326514
[oral triple therapy for eradication of helicobacter pylori in duodenal ulcer disease].	25 patients with helicobacter pylori-associated active duodenal ulcer disease (bleeding: n = 5, penetrating: n = 1, stenosis of the bulb: n = 4, frequent ulcer relapse: n = 18) were treated with 3 x 600 mg bismuth subsalicylate (bss), 3 x 400 mg metronidazole and 3 x 500 mg tetracycline in addition to 300 mg ranitidine. 23 out of 25 patients (92%) proved to be helicobacter pylori-negative four weeks after cessation of study medication as judged from negative urease test, specific culture and his ...	1993	8326916
the us temporal and geographic variations of diseases related to helicobacter pylori.	if helicobacter pylori plays an important role in the occurrence of three distinct diseases--gastric cancer, gastric ulcer, and duodenal ulcer--their individual epidemiologic behaviors should show a resemblance to one common pattern.	1993	8328594
epidemiology of helicobacter pylori: the indian scenario.	to compare the age-related prevalence of helicobacter pylori in populations from developing and developed nations to determine the possible mode of transmission.	1993	8330925
identification of helicobacter pylori dna in the mouths and stomachs of patients with gastritis using pcr.	to determine the prevalence of helicobacter pylori colonisation in the mouths of patients with h pylori gastritis.	1993	8331177
effect of helicobacter pylori infection on intragastric urea and ammonium concentrations in patients with chronic renal failure.	to assess the value of measuring the gastric juice urea:ammonium ratio in detecting helicobacter pylori infection in patients with chronic renal failure.	1993	8331178
effect of transport medium and transportation time on culture of helicobacter pylori from gastric biopsy specimens.	to determine whether transportation time and use of a low budget transport medium (nacl 0.9%) would influence culture of helicobacter pylori from gastric biopsy specimens, upper gastrointestinal endoscopy was performed on 42 patients. the specimens were cultured and examined histologically, and h pylori antibodies were determined using an elisa technique. patients were regarded as h pylori positive when culture was positive or when histology or igg anti-h pylori antibodies indicated h pylori inf ...	1993	8331182
clarithromycin for the eradication of helicobacter pylori.	helicobacter pylori infection has proven to be extraordinarily difficult to eradicate. antimicrobial monotherapies have been particularly disappointing, with most eradication rates in the range of 0-15%. clarithromycin has potential advantages over other macrolides to which h. pylori is susceptible because of its acid stability and solubility at low ph. we evaluated clarithromycin therapy (250 mg four times daily for 14 days) in 12 h. pylori-infected patients. the 13c-urea breath test was used t ...	1993	8331260
aflagellated mutants of helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis.	three out of 10 helicobacter pylori clinical isolates were found to be naturally competent for genetic transformation to streptomycin resistance by chromosomal dna extracted from a spontaneous streptomycin-resistant h. pylori mutant. the frequency of transformation varied between 5 x 10(-4) and 4 x 10(-6), depending on the h. pylori isolate used. transposon shuttle mutagenesis based on this natural competence was established using the flagellin gene flaa as the target. the cloned flaa gene was i ...	1993	8332066
immune suppressive effects of helicobacter pylori on human peripheral blood mononuclear cells.	helicobacter pylori, the causative agent of type-b gastritis and duodenal ulcer in man is described as a bacterium able to stimulate the human immune system. this study demonstrates that h. pylori besides this property possesses an immune suppressive activity. the in vitro proliferation of human peripheral blood mononuclear cells to purified protein derivative of tuberculin (ppd), phytohemagglutinin, and concanavalin a was reduced in a dose-dependent manner by bacteria which had been inactivated ...	1993	8332102
helicobacter pylori in renal transplant recipients.	an unselected group of 33 renal transplant recipients were examined by upper gastrointestinal endoscopy at between two and four months after transplantation. all abnormal lesions were documented and biopsied and, in addition, random biopsies were obtained from the gastric antrum and from the first part of the duodenum. the biopsies were examined and graded for gastritis and duodenitis and the presence of helicobacter pylori was noted. duodenitis was identified in 16 patients and gastritis in 10; ...	1993	8333032
antibiotic therapy alone eradicates helicobacter pylori and prevents duodenal ulcer recurrence.		1993	8335215
sequence of the proteus mirabilis urease accessory gene ureg.	we report the sequence of ureg, an accessory gene that is a part of the ure gene cluster of uropathogenic proteus mirabilis and required for full enzymatic activity of urease. the 615-bp open reading frame predicts a m(r) 22,374 polypeptide, which contains a consensus amino acid (aa) sequence for atp-binding. the polypeptide shares sequence homology with ureg of escherichia coli (93% of identical aa), klebsiella aerogenes (59%) and helicobacter pylori (59%).	1993	8335248
an uncultured gastric spiral organism is a newly identified helicobacter in humans.	"gastrospirillum hominis" is an uncultivated spiral bacterium in human gastric mucosa that is larger and more tightly coiled than helicobacter pylori. in an attempt to determine if this organism is a new species of helicobacter, its 16s rrna gene was cloned and sequenced. gastric mucosa from 2 patients infected with "gastrospirillum hominis" was fed to specific pathogen-free mice. electron microscopy of gastric tissue confirmed that the mice became colonized with "gastrospirillum hominis." the 1 ...	1993	8335974
histamine content of the oxyntic mucosa from duodenal ulcer patients: effect of helicobacter pylori eradication.	the histamine concentration of the oxyntic mucosa was determined in helicobacter pylori-positive patients with duodenal ulcer before and after antimicrobial therapy and in h. pylori-negative subjects. determination of serum gastrin was also performed in duodenal ulcer patients before and after h. pylori eradication. the histamine content of the oxyntic mucosa was lower in patients with duodenal ulcer than in h. pylori-negative subjects, but it increased after h. pylori eradication. conversely, i ...	1993	8338090
apparent reversal of early gastric mucosal atrophy after triple therapy for helicobacter pylori.	helicobacter pylori may be difficult to detect in individuals with intestinal metaplasia or atrophic gastritis, even though bacteria may persist in the mucosa in low numbers, maintaining elevated serum h. pylori antibody levels. we report a patient with marked, endoscopically visible gastric mucosal changes and focal changes of histological atrophic gastritis, who was negative for h. pylori on urease test, culture, and histology, but had positive h. pylori serology. when treated with triple ther ...	1993	8338096
low prevalence of helicobacter pylori in the acquired immunodeficiency syndrome.		1993	8338111
proton-pump inhibitor-refractory gastric ulcer and helicobacter pylori.		1993	8338112
source of helicobacter pylori infection.		1993	8338115
[detection of serum igg (elisa) against helicobacter pylori: relationship with age and gastroduodenal disease].	an attempt was made to investigate the serological response against helicobacter pylori by using a igg serum detection technique (elisa biometra) to establish a relationship with age and gastroduodenal pathology. the serological response was compared with the microbiologic and histologic studies of biopsy samples from 4 locations in each patient: duodenal bulb, gastric antrum, corpus and fundus.	1993	8338704
identification of helicobacter pylori by endoscopic crush cytology.	gastric crush cytology is employed in a variety of situations including diagnosis of malignant disease and the detection of helicobacter pylori infection.	1993	8340141
role of acid inhibition in the management of helicobacter pylori infection: chairmen's introduction.		1993	8341985
hp and ph: implications for the eradication of helicobacter pylori.	helicobacter pylori infection causes inflammation of the gastric and duodenal mucosae, which results in a disturbance of the regulatory mechanisms for gastrin, gastric acid and pepsin secretion. acid secretion may be decreased, normal or increased, depending on the stage of infection, although the meal-stimulated gastrin response is invariably elevated. the exact mechanisms involved are not known but may be due to the release of cytokines in response to bacterial toxins. h. pylori colonization i ...	1993	8341986
long-term follow-up after eradication of helicobacter pylori with a combination of omeprazole and amoxycillin.	early studies have suggested that omeprazole may facilitate the eradication of helicobacter pylori. sixty patients with duodenal ulcer and h. pylori colonization were randomly assigned to receive either omeprazole monotherapy (n = 30) or combination therapy with omeprazole and amoxycillin (n = 30) for a total duration of 6 weeks. four patients receiving monotherapy and three receiving combination therapy had to be withdrawn from the study. all (100%) duodenal ulcers healed in patients receiving ...	1993	8341987
role of helicobacter pylori in the pathogenesis of gastritis, peptic ulcer and gastric cancer.	helicobacter pylori is the major causal factor in chronic gastritis. its acquisition leads to a chronic, usually lifelong, inflammation of the gastric mucosa, which may gradually progress to atrophy (with intestinal metaplasia) in a significant proportion of infected individuals. this progression is probably multifactorial, being influenced by genetic or environmental factors in addition to h. pylori infection. the pathogenesis of peptic ulcer and gastric cancer is closely associated with h. pyl ...	1993	8341988
compliance, adverse events and antibiotic resistance in helicobacter pylori treatment.	the highest h. pylori eradication rates have been reported with triple therapy, using metronidazole with amoxycillin or tetracycline, and colloidal bismuth subcitrate or bismuth subsalicylate. the use of such therapies, however, may be impeded by a number of major disadvantages, including reduced patient compliance, the incidence of adverse events and primary or acquired antibiotic resistance. patient compliance is a particular problem with triple therapy owing to the quantity of drugs taken, tr ...	1993	8341989
eradication of helicobacter pylori and its effect in peptic ulcer disease.	since helicobacter pylori was first cultured 10 years ago, there have been remarkable changes in our approach to the therapy of peptic ulcer disease. we now know that 90% of duodenal ulcer patients and 70% of all gastric ulcer patients are infected with h. pylori. evidence is presented that the relapse rate of both duodenal and gastric ulcers can be substantially reduced if the bacterium is eradicated from the patient's stomach. some of the anti-h. pylori eradication regimens currently available ...	1993	8341990
gastric syphilis. primary diagnosis by gastric biopsy: report of four cases.	gastric involvement in secondary or tertiary syphilis is rarely recognized clinically, and its diagnosis by examination of endoscopic biopsy specimens has been reported infrequently. we report four cases of gastric syphilis with the primary diagnosis made by gastric biopsy. the patients, all male, ranged in age from 38 to 78 years and presented with gastric complaints, the most common being upper gastrointestinal tract bleeding (three of four). gastroscopy showed either erosive gastritis or gast ...	1993	8343046
acid secretion and sensitivity to gastrin in patients with duodenal ulcer: effect of eradication of helicobacter pylori.	the effect of ulcer healing with eradication of helicobacter pylori (h pylori) on gastric function was investigated in nine patients with duodenal ulcer disease. one month after eradication there were significant reductions in both basal plasma gastrin concentration, from a median (range) of 19 (1-22) to 6 (2-15) pmol/l (p < 0.05), and of basal acid secretion from 8.3 (2.4-24) to 2.6 (1.4-8.1) mm h+/h, (p < 0.01). the peak acid secretion rate was unchanged from 37 (16-59) to 37 (21-59) mm h+/h. ...	1993	8344574
changes in the gastric mucosa following eradication of helicobacter pylori.	although most studies reporting on the examination of helicobacter pylori infection have focused on the clearance of the bacteria and the rapid disappearance of the neutrophil infiltrates, the evolution of inflammatory and architectural changes in the antral and corporal mucosa following the eradication of h. pylori has not been addressed systematically. this study examines in detail the histopathologic appearance of the antral and corporal mucosa in a group of patients infected with h. pylori a ...	1993	8346176
eradication of helicobacter pylori in recurrent duodenal ulcer.		1993	8347229
helicobacter pylori, dietary factors, and atrophic gastritis in five japanese populations with different gastric cancer mortality.	in a cross-sectional study of 634 men aged 40 to 49 years, randomly selected from five areas of japan with different rates of gastric cancer mortality, 121 men of 624 evaluated were diagnosed as having atrophic gastritis through serum pepsinogen i < 70 ng/ml and the pepsinogen i (pgi)/pepsinogen ii (pgii) ratio < 3.0. we examined the relation of helicobacter pylori (h. pylori) antibodies and dietary factors, including plasma level of antioxidant micronutrients, to the presence of atrophic gastri ...	1993	8347778
helicobacter pylori infection and chronic atrophic gastritis among japanese blood donors: a cross-sectional study.	to evaluate an association between helicobacter pylori (h. pylori) infection and chronic atrophic gastritis (cag), an established precursor of gastric cancer, we performed a cross-sectional study using igg antibody against h. pylori and pepsinogens of blood donors in four prefectures in japan. although a geographic correlation between the age-adjusted prevalence rates for h. pylori infection and those for cag was not seen, the age-adjusted odds ratios (or) of h. pylori infection for cag were hig ...	1993	8347779
helicobacter pylori infection--when and how to treat.		1993	8348879
social and geographical risk factors in helicobacter pylori infection.	a correlation between childhood crowding and the later development of gastric cancer has been demonstrated by barker and colleagues, who proposed that the relationship was the consequence of infection by an organism such as helicobacter pylori. in order to test this hypothesis the presence of igg antibodies to h. pylori in sera from blood donors in north wales has been investigated. during donation sessions, donors answered questions relating to social conditions and domicile in childhood (at ag ...	1993	8348934
diagnosis of helicobacter pylori infection in adults and children by using the malakit helicobacter pylori, a commercially available enzyme-linked immunosorbent assay.	malakit helicobacter pylori (biolab, limal, belgium) is a second-generation enzyme-linked immunosorbent assay (elisa) for the detection of helicobacter pylori infection. we evaluated its ability to diagnose h. pylori infection in 489 asymptomatic pregnant women, 427 asymptomatic children, and 95 symptomatic children. 87 asymptomatic adults (17.8%), 31 asymptomatic children (7.3%), and 27 symptomatic children (28.4%) were seropositive. we observed an increase in h. pylori infection with age. 13c- ...	1993	8349752
novel campylobacter-like organism resembling helicobacter fennelliae isolated from a boy with gastroenteritis and from dogs.	we isolated a campylobacter-like organism resembling helicobacter fennelliae from a 5 1/2-year-old boy with gastroenteritis. similar strains had been found previously in fecal specimens from healthy and diarrheic dogs. these isolates could be differentiated from h. fennelliae by a lack of catalase and arylsulfatase activities. this group of organisms seems to be homogeneous by a nonradioactive dot blot dna hybridization assay.	1993	8349774
sensitivity of culture compared with that of polymerase chain reaction for detection of helicobacter pylori from antral biopsy samples.	polymerase chain reaction (pcr) was used to test the sensitivity of standard bacteriological culture of helicobacter pylori. the tests were concordant in 97% of cases. pcr did not detect more treatment failures after triple treatment than did the standard culture method. in the present study, culture was as sensitive as pcr for the detection of h. pylori.	1993	8349775
helicobacter pylori infection in children.		1993	8350206
helicobacter pylori eradication.		1993	8351270
[helicobacter pylori gastritis manifested by acute anemia].	helicobacter pylori gastritis usually manifests as recurrent abdominal pain but is sometimes discovered upon evaluation for digestive tract bleeding with severe anemia. an 11-year-old who was not under medication and had no history of pain was admitted for isolated regenerative anemia (5.6 g/dl) due to digestive tract bleeding. laboratory tests showed only low serum iron and ferritin levels. endoscopy disclosed hemorrhagic inflammation of the duodenal cap and antritis with a hillocky appearance. ...	1993	8352498
[a study on the relationship between helicobacter pylori and chronic gastritis and peptic ulcer by the case-control method and sequential analysis].	the association between helicobacter pylori and the chronic gastritis and peptic ulcer was investigated by the case-control method, 1:1 matched study and sequential analysis. the results of three methods showed that the degree of association between helicobacter pylori and the chronic gastritis and peptic ulcer was significant (chi 2 = 22.70, p < 0.001, or = 15.63, 95% ci of or was 5.05, 48.43), especially that between helicobacter pylori and active chronic gastritis (or = 20.92). the results in ...	1993	8353820
[helicobacter pylori infection and ulcer disease].		1993	8354141
detection of helicobacter pylori in gastric biopsy tissue by polymerase chain reaction.	to evaluate the sensitivity of a polymerase chain reaction (pcr) assay using nested primers in detecting helicobacter pylori, gastric tissue biopsy specimens were collected on endoscopy from 17 patients with a duodenal ulcer. dna was extracted by phenol/chloroform treatment or boiling in water, and then subjected to a nested pcr using two primer pairs from the urease gene of helicobacter pylori. fourteen of the 17 patients were positive for helicobacter pylori using dna samples extracted by eith ...	1993	8354305
kinetics of specific igg antibodies for monitoring the effect of anti-helicobacter pylori chemotherapy.	a total of 104 patients with recurrent duodenal ulcer were treated with either ranitidine plus amoxicillin plus metronidazole or ranitidine plus placebo. to study the effect of the eradication of helicobacter pylori on the systemic immune response in an igg elisa, sera were drawn from all patients before the onset of therapy and at 6, 16 +/- 2, 32 +/- 2, and 60 +/- 2 weeks after therapy. in patients with eradication of the organism, a significant (p < .001) reduction of the specific igg titer oc ...	1993	8354918
rolling circle-replicating plasmids from gram-positive and gram-negative bacteria: a wall falls.	rolling circle-replicating plasmids constitute a group of small, promiscuous multicopy replicons spread among eubacteria. until recently, rolling circle replication seemed to be limited to small plasmids from gram-positive hosts and to single-stranded bacteriophages from gram-negative bacteria. however, characterization of two small plasmids from gram-negative hosts has shown that this replication mechanism is general among eubacteria. this review focuses on a family of highly related promiscuou ...	1993	8355606
[laboratory diagnostic experience in pyloric helicobacteriosis].		1993	8356823
serologic response to helicobacter pylori among children and teenagers in northern chile.	the serologic response to helicobacter pylori was determined in 388 children and teenagers living in iquique, chile by using an igg enzyme-linked immunosorbent assay. serum antibody levels, as measured by optical density, correlated strongly with age. increases in the mean antibody level were seen primarily after age five, with rates of seropositivity increasing to > or = 70% among teenagers. the reasons for this age-related pattern of acquisition of infection remain to be determined.	1993	8357081
[barrett++ esophagus in children. presentation of 12 cases].	this paper describes 12 cases of barrett's oesophagus selected out of a population of 364 refluxing children studied from 1986 to 1990. their mean age was 14, seven were cared for in special institutions for brain damaged and 2 had been operated upon for oesophageal atresia at birth. excessive acid exposure was documented in 8/10 patient studied and simultaneous gastric and oesophageal ph-monitoring demonstrated massive duodeno-gastric alkaline reflux in 3. histological studies revealed that col ...	1993	8357725
prevalence of helicobacter pylori infection in patients with precancerous changes and gastric cancer.	several papers suggested a role for h. pylori infection in gastric cancer. we evaluated the prevalence of h. pylori infection in an endoscopic population of patients with gastric precancerous conditions and lesions by studying biopsies from 252 patients and recording the presence and degree of h. pylori infection. patients were subgrouped as follows: chronic non-atrophic gastritis (cg), chronic atrophic gastritis (cag), intestinal metaplasia (im), epithelial dysplasia (ed) and gastric cancer (k) ...	1993	8358284
[the effect of omeprazole on healing of duodenal ulcers, helicobacter pylori and gastritis].	losec (omeprazole) astra co. is a blocker of the proton pump of the parietal cell. it inhibits basal and stimulated hcl secretion. it is used for treatment of gastroduodenal ulcers, reflux oesophagitis and zollinger ellison's syndrome. in a group of 17 patients with duodenal ulcers the authors investigated the effect of omeprazole on (1) healing of duodenal ulcers and bulbitis after 2-4 weeks of therapy, (2) elimination of helicobacter pylori in the antrum, (3) chronic antral gastritis. ad 1. af ...	1993	8358765
one-day therapy for treatment of helicobacter pylori infection.	the present study evaluated the effect of a one-day high-dose combined therapy on helicobacter pylori infection. thirty-two consecutive patients (suffering from either peptic ulcer or nonulcer dyspepsia) with helicobacter pylori infection received omeprazole (40 mg) + bismuth subcitrate (240 mg x 4) + amoxicillin suspension (2000 mg x 4) + metronidazole (500 mg x 4), for only one day. endoscopy, histology, culture, and susceptibility studies were done at entry and 30 and 90 days after the treatm ...	1993	8359079
bismuth subsalicylate suppression of helicobacter pylori in nonulcer dyspepsia: a double-blind placebo-controlled trial.	gastritis caused by helicobacter pylori (hp) is common in patients with nonulcer dyspepsia (nud), but an etiologic relationship between the histologic lesion and clinical symptoms is unproven. hp is inhibited by bismuth subsalicylate (bss), a traditional remedy for dyspeptic complaints. the aim of this study was to assess the short- and long-term effects of bss on hp, gastritis, and symptoms in patients with nud. one hundred twenty-six patients with nud who were shown to be infected with h. pylo ...	1993	8359080
asymptomatic h. pylori infection impairs ph inhibition of gastrin and acid secretion during second hour of peptone meal stimulation.	h. pylori infection is associated with acid-peptic disease, although its role in the pathogenesis is unclear. the purpose of this study was to determine if chronic infection in asymptomatic subjects impairs the inhibition of meal-stimulated gastrin and acid secretion that is observed normally at low intragastric ph. presence of infection was determined by both c-14 urea breath test and serology. acid secretion was measured under basal conditions and in response to peptone meal stimulation and pe ...	1993	8359081
helicobacter pylori infection, abo blood group, and effect of misoprostol on gastroduodenal mucosa in nsaid-treated patients with rheumatoid arthritis.	our aim was to investigate the effect of misoprostol on nsaid-induced gastroduodenal mucosal damage in patients with rheumatoid arthritis. the study included 40 patients, and it was designed as a double-blind, placebo-controlled trial. misoprostol significantly reduced the gastroduodenal mucosal lesions found at endoscopy (p < 0.05) and prevented the development of ulcers. the cumulative incidence of ulcers at four weeks was 5% in the placebo group and 0% in the misoprostol group. the basal and ...	1993	8359082
production of chemoattractant by helicobacter pylori.	helicobacter pylori is present in the antral region of the stomach in a majority of patients with gastritis type b. the specific mechanism whereby the organism participates in the development of disease remains uncertain. since the organism is not invasive, we postulate that h. pylori produces a chemoattractant that recruits inflammatory cells to the antral region of the stomach. h. pylori was grown under microaerophilic conditions at 37 degrees c for 72 hr in brucella broth containing 1% fetal ...	1993	8359083
congestive gastropathy versus chronic gastritis: a comparison of some pathophysiological aspects.	in order to investigate some pathophysiological aspects of the two diseases, 15 patients with congestive gastropathy and 15 with chronic gastritis have been studied and compared with 15 healthy controls. gastric blood flow as determined during endoscopy by means of laser doppler flowmetry was found to be significantly increased (p < 0.001) in congestive gastropathy, whereas a significant reduction (p < 0.001) was noted in chronic gastritis. on the other hand, helicobacter pylori was detected in ...	1993	8359558
epidemiology and genetics of peptic ulcer.	at the turn of the century, duodenal ulcer rose from rarity to affect 10% of males in their life time, subsequently declining in some countries such as uk, levelling off in others such as germany, and continuing to increase in still others such as hong kong. the annual incidence per 1000 population varies from about 1 in japan to 1.5 in norway, 1.8 in usa and 2.7 in scotland, and the frequency also varies within many individual countries, such as australia, china and india, and among races such ...	1993	8359624
the role of helicobacter pylori in peptic ulcer disease.	the linking of relapse of duodenal as well as gastric ulcers with helicobacter pylori (h. pylori) has been a considerable advance in managing patients with peptic ulcer disease. however, pathogenetic role of h. pylori in peptic ulcer still remains unclear. the aim of this study was to assess the relationship between h. pylori infection and inflammatory cell infiltration in gastric mucosa in peptic ulcer disease. sixty-four patients with endoscopically proven gastric ulcer and 26 patients with du ...	1993	8359625
an immunofluorescent stain for helicobacter pylori.	we prepared a murine monoclonal antibody that recognized the helicobacter pylori urease. monoclonal antibody, u2, reacted with purified native urease in enzyme linked immunosorbent assay but it did not react in western immunoblot analysis. urease was immunoprecipitated with u2 bound to staphylococcus aureus protein a, followed by elution and visualization by sodium dodecyl-sulfate polyacrylamide gel electrophoresis. the species and strain specificity of u2 were determined by biodot reaction with ...	1993	8359826
[the role of helicobacter pylori in the etiology of peptic ulcer and its therapy].	the isolation of helicobacter pylori from human gastric mucosa by warren and marshall in 1984, has given new approaches in etiology of peptic ulceration. approximately all of duodenal ulcer patients have displayed antral gastritis with helicobacter pylori, made strong suspicion of the bacteria in the etiology of duodenal ulcer. in therapy, combination of antibiotics to acid suppressive therapy should be preferred to antibiotics only.	1993	8361417
the role of helicobacter pylori in peptic ulcer disease.	the pathophysiology of peptic ulcer disease (pud) is often described as an imbalance between aggressive factors such as acid and pepsin and alterations in the mucosal protective mechanisms. helicobacter pylori is a gram-negative organism that has been identified as a potential causative agent in the pathogenesis of pud. the exact mechanism by which it contributes to mucosal damage is unknown. it is thought that the organism may disrupt the protective mucous layer, allowing the underlying epithel ...	1993	8361859
helicobacter pylori and peptic ulcer disease. reexamining the therapeutic approach.	now that helicobacter pylori has been strongly implicated as a causative factor in peptic ulcer disease, the dictum "no acid--no ulcer" may no longer be valid. however, h pylori has proved difficult to eradicate, and the approach to treatment of peptic ulcer is undergoing changes. at present, a triple-drug combination of bismuth and antibiotics is advised, and cultures should be obtained before and after treatment to monitor its efficacy. side effects include pseudomembranous colitis and drug re ...	1993	8361941
helicobacter pylori infection in peptic ulcer disease.		1993	8362207
long-term helicobacter pylori recurrence after successful eradication with triple therapy.	to establish the rate of helicobacter pylori recurrence after a standard triple-therapy regimen (bismuth subsalicylate, tetracycline, metronidazole) and determine which clinical factors affect reinfection, we prospectively followed 118 patients after successful h. pylori eradication. elimination of h. pylori was confirmed by repeat endoscopy and urea breath test 4 wk after completion of therapy. serial [13c]urea breath tests were performed at 3-month intervals; antral biopsies were used to verif ...	1993	8362830
gastric mucosal hydrophobicity and helicobacter pylori: response to antimicrobial therapy.	the hydrophobic properties of the gastric mucosa are reduced by nsaids and by helicobacter pylori infection. our investigation was to determine whether this abnormality was due to the bacteria or to the inflammatory response. contact angle measurements were made on gastric antral and corpus biopsies taken from 10 h. pylori-infected volunteers before eradication therapy, after 2 and 14 days of therapy, and 4 weeks after therapy. the contact angle improved steadily and statistically throughout the ...	1993	8362831
evaluation of new therapy for eradication of h. pylori infection in nude mouse model.	developing a new therapy for eradication of h. pylori by using the nude mouse model.	1993	8362832
helicobacter pylori and intestinal metaplasia: comparison between british and yemeni patients.	there have been suggestions linking gastric carcinoma with helicobacter pylori on the one hand and type iii intestinal metaplasia on the other hand. this study was aimed at investigating the relationship between intestinal metaplasia and its subtypes, and the presence or absence of h. pylori in gastric biopsies from two geographically different patient populations, one with a much higher prevalence of h. pylori than the other. antral biopsies from 179 british and 123 yemeni patients with dyspeps ...	1993	8362833
local gastric and serum amoxicillin concentrations after different oral application forms.	the high recolonization rate after monotherapy of helicobacter pylori-positive gastritis may be due to insufficient local drug concentrations. to investigate the role of local diffusion, we measured levels of amoxicillin, a drug with good in vitro activity against h. pylori, in the mucosa and serum. one gram of amoxicillin was given to healthy volunteers as a tablet (n = 6) or as water-dissolved, fizzing "tab" (n = 6). gastroscopy with biopsies from the antrum, corpus, and fundus was performed a ...	1993	8363383
helicobacter pylori infection in an hiv-seropositive patient with diarrhoea.		1993	8363772
gastric anti-secretory, mucosal protective, anti-pepsin and anti-helicobacter properties of ranitidine bismuth citrate.	ranitidine bismuth citrate is a novel compound formed from ranitidine and a bismuth citrate complex. in conscious dogs, ranitidine bismuth citrate had similar activity to ranitidine hydrochloride as an inhibitor of histamine-induced gastric acid secretion when oral doses containing equivalent amounts of ranitidine base (0.1 or 0.3 mg/kg) were compared. in the rat, ranitidine bismuth citrate (3-30 mg/kg p.o.) prevented gastric mucosal damage induced by ethanol (fundic damage) and indomethacin (an ...	1993	8364129
short report: high-dose omeprazole and amoxycillin in the treatment of helicobacter pylori-associated duodenal ulcer.	thirteen consecutive patients with active duodenal ulcer disease were assigned to a treatment schedule with high-dose omeprazole and amoxycillin. duodenal ulcer was diagnosed endoscopically in all patients, at which time antral biopsies were taken for culture and histology. all were positive for helicobacter pylori and histological gastritis. treatment was for 2 weeks: 80 mg omeprazole daily plus 500 mg amoxycillin syrup t.d.s. in the first week, followed by 40 mg omeprazole daily in the second ...	1993	8364136
the need for long-term treatment of peptic ulcer.	some 10% of the population in western countries will suffer a duodenal ulcer or gastric ulcer at some time in their lives. although there has been an improvement in the survival rate of patients with peptic ulcer haemorrhage, the mortality is still approximately 10%. there is evidence to suggest that peptic ulcer disease is a life-long condition and that ulcers remain active with an unchanged potential for complications such as haemorrhage and perforation. over the past 15 years anti-ulcer drugs ...	1993	8364141
management issues for debate: the problems in perspective.		1993	8364142
primary prevention of gastric precancerous lesions.		1993	8364376
high-affinity binding of laminin by helicobacter pylori: evidence for a lectin-like interaction.	laminin, the major glycoprotein of basement membranes, was shown to be bound by the human gastric pathogen helicobacter pylori. binding of 125i-laminin by strain 17874 was time-dependent, specific and saturable. scatchard analysis of specific binding indicated about 2000 binding sites per cell with a dissociation constant of 8.5 pm. treatment of the cells by heat (80 degrees) and with proteolytic enzymes drastically reduced laminin binding, suggesting that the laminin receptors are surface prote ...	1993	8364520
colloidal bismuth subcitrate, ranitidine, and ranitidine plus metronidazole in the treatment of duodenal ulcer and helicobacter pylori infection: a controlled and prospective study.	a controlled, prospective clinical trial of colloidal bismuth subcitrate (cbs), ranitidine and ranitidine plus metronidazole for the treatment of duodenal ulcer (du) is reported here, with evaluation of the possible pathogenic role of helicobacter pylori (hp) on du in a six-month follow-up. a total of 42 patients with active du on endoscopy were randomly selected to receive oral cbs (300 mg 1 hour before each meal, and at bedtime, n = 16), ranitidine (150 mg bid; n = 12) or ranitidine (150mg b.i ...	1993	8364776
the association of helicobacter pylori with differentiated-type early gastric cancer.	recent studies have indicated that infection with helicobacter pylori is closely associated with increased risk for gastric cancer. previous studies, however, usually have been made on cases of advanced gastric cancer.	1993	8364862
granulomatous gastritis: a morphological and diagnostic approach.	the final diagnosis of granulomatous gastritis is based on morphological findings and clinical and laboratory data. detailed analysis of the morphological features of the granulomas together with associated mucosal changes could generate more information on aetiology and pathogenesis. biopsies from 71 patients diagnosed as having granulomatous gastritis were reviewed. thirty-seven of these patients (52%) had crohn's disease. in 18 patients (25%) an isolated granulomatous gastritis was diagnosed. ...	1993	8365714
[stomach ulcer and duodenal ulcer].		1993	8365864
reduced incidence of stress ulcer in germ-free sprague dawley rats.	recent findings with respect to the role of spiral gram-negative bacteria in peptic ulcer disease have stimulated interest in discerning the role of these agents in stress ulcer disease. we tested the hypothesis that a standard restraint-cold ulcerogenic procedure would fail to produce ulcers in axenic rats. axenic, as well as normal sprague dawley rats, were exposed to a cold-restraint procedure. the germ-free condition was maintained throughout the study in the axenic rats. axenic rats had sig ...	1993	8366772
[natural history of duodenal ulcer].	the authors have studied the natural history of duodenal ulcer from published data, in an attempt to determine precisely the evolutive potential of this disease both spontaneously and during its treatment. they deal successively with the risk of complication and the short- or long-term influence of medical and surgical treatment on the occurrence of exacerbations and complications. the role played by helicobacter pylori in healing of the ulcer and in the long-term course of the ulcerous disease, ...	1993	8367419
[serologic detection of helicobacter pylori antibodies after surgery for stomach and duodenal ulcer].	the occurrence of igg antibodies to helicobacter pylori was measured by means of elisa in 175 patients who had undergone surgery for duodenal or gastric ulcer. there was a significant correlation between surgery with resection and the absence of igg to helicobacter pylori in about 80% of cases. in patients undergoing surgery without resection there was no influence of the surgical technique on the presence of helicobacter pylori antibodies.	1993	8367977
biochemical and histopathological aspects in duodenogastric reflux gastritis patients with or without prior cholecystectomy.	the aim of this study was to evaluate some biochemical and histopathological aspects in a group of patients with a view to identifying any differences depending on whether the pathology was associated with previous cholecystectomy or idiopathic. the study involved 23 patients (8 post-cholecystectomy cases and 15 ulcer-free dyspeptic patients) with the diagnosis of duodenogastric reflux gastritis confirmed by endoscopic histopathological evaluation. the following parameters were considered: 1. ph ...	1993	8368047
[colloidal bismuth subcitrate and amoxicillin in the treatment of helicobacter pylori infection in children].	we have analyzed the effect of treatment with colloidal bismuth subcitrate (cbs) and amoxicillin in a series of 60 children (age range: 2.9-18 years) infected by helicobacter pylori (hp). the following results were found: clinical remission 70%; endoscopic normalization 55%; histological normalization 61.6% and hp eradication 53.3%. the improvement of histological activity (100% vs 11.7%) and histological normalization (90.6% vs 28.5%) were significantly more frequent in the hp eradicated group ...	1993	8368681
metronidazole susceptibility testing for helicobacter pylori: comparison of disk, broth, and agar dilution methods and their clinical relevance.	since the methods for metronidazole susceptibility testing of helicobacter pylori have not been standardized or validated, we compared three methods that are used to test the metronidazole susceptibilities of 25 isolates of h. pylori. specifically, we examined the methods of steer's replicator agar dilution, tube broth microdilution, and modified kirby-bauer disk diffusion. the metronidazole disk zone sizes obtained by the disk diffusion method correlated well (r = 0.74) with the mics obtained b ...	1993	8370723
a highly specific and sensitive dna probe derived from chromosomal dna of helicobacter pylori is useful for typing h. pylori isolates.	hindiii-digested dna fragments derived from an ecori-digested 6.5-kb fragment of chromosomal dna prepared from helicobacter pylori atcc 43629 (type strain) were cloned into the puc19 vector. a 0.86-kb insert was identified as a potential chromosomal dna probe. the specificity of the probe was evaluated by testing 166 non-h. pylori bacterial strains representing 38 genera and 91 species which included aerobic, anaerobic, and microaerophilic flora of the upper and lower gastrointestinal tracts. no ...	1993	8370744
detection of immunoglobulin g antibodies to helicobacter pylori in urine by an enzyme immunoassay method.	urine and serum samples from 306 patients undergoing upper endoscopy were evaluated prospectively to determine the presence of immunoglobulin g (igg) antibodies to helicobacter pylori by an enzyme immunoassay method. forty-nine selected urine specimens were also tested by western blotting (immunoblotting). when compared with bioptic methods (culture, stain, urease testing), the sensitivity and specificity of the assay for urine igg to h. pylori were 95.9 and 90%, respectively. results of testing ...	1993	8370747
susceptibility of helicobacter pylori and pharmacokinetic properties in mice of new 5-nitroimidazole derivatives devoid of mutagenic activity in the ames test.	the minimum inhibitory concentrations of metronidazole and four new 5-nitroimidazole derivatives (eu 11100, eu 11102, eu 11103, eu 11104), obtained by the reaction of 1-methyl-5-nitroimidazolyl-2-carboxyaldehyde and terbutyl-phenol, were determined against 25 clinical isolates of helicobacter pylori. three of them (eu 11100, eu 11103, eu 11104) exhibited an antibacterial activity higher than that of metronidazole. the last one, the molecule eu 11102, was less active than metronidazole. in mice, ...	1993	8371126
[costs and effectiveness of diagnosis and treatment of patients with dyspepsia, determined with the aid of a computer model].	a theoretical investigation was carried out to determine whether the use of an antibody test for helicobacter pylori (hp) and the use of bismuth oxide with optional antibiotics, would be cost effective in the diagnosis and treatment of patients with dyspepsia. with literature data on therapeutic effectiveness a computer simulation was carried out. a quick antibody test reduced the number of gastroscopic investigations necessary. gastroscopic investigation still had to be carried out for patients ...	1993	8371821
[ranitidine therapeutically effective in functional dyspepsia with reflux-like symptoms].	to investigate the effect of ranitidine in patients with functional dyspepsia according to different subgroups.	1993	8371822
fluorinated probes to measure carboxylesterase activities using 19f nmr spectroscopy: application to erythrocytes and helicobacter pylori.	eight fluorinated compounds were tested as putative probes to measure carboxylesterase activity employing 19f nuclear magnetic resonance spectroscopy. the method takes advantage of the sensitivity of fluorine resonances to the changes in the chemical bonding in the covalent structures where they are located. determination of the kinetic parameters of ethyl fluoroacetate and diethyl fluoromalonate in hemolysates showed that these probes were well suited to study carboxylesterase activities in com ...	1993	8373162
[the clinico-endoscopic characteristics of gastric erosions and their relationship to helicobacter pylori lesions].	fibrogastroduodenoscopy was carried out in 285 patients. in all cases with gastric erosions chromograstroscopy was done with the purpose of revealing the acid-production function of the stomach. helicobacter pylori was revealed mainly in patients with "complete" erosions against the background of chronic gastritis b.	1993	8379140
use of serum specific immunoglobulin antibodies to determine helicobacter pylori associated gastritis.	a prospective study in 169 consecutive patients referred for upper gastrointestinal endoscopy was initiated to investigate the diagnostic performance of serum helicobacter pylori (hp) specific immunoglobulin (ig) g antibodies. using an enzyme linked immunosorbent assay (elisa) an excellent correlation between serologic evidence of hp and the demonstration of this organism by histology and urease test in 79 hp-positive patients was found. serum igg also correlated with the histological degree and ...	1993	8379229
[prevalence of helicobacter pylori and gastritis in southern germany. results of a representative cross-sectional study].	the recent discussions about the relationship between helicobacter-pylori-infection, gastritis and the occurrence of stomach cancer caused us to analyse blood sera of 543 participants randomly selected from the general population in the age of 25 to 34 and of 55 to 64 years from mosbach (neckar-odenwald-kreis), the county of deggendorf (lower-bavaria) and augsburg (upper-bavaria) regarding igg-antibodies against helicobacter pylori and the concentrations of the pepsinogens a and c. the latter we ...	1993	8379230
[qualitative and semiquantitative invasive and noninvasive diagnosis of helicobacter pylori colonization of gastric mucosa].	in 110 patients with unknown helicobacter pylori status and without anti-bacterial treatment submitted for routine endoscopy of the upper gastrointestinal tract biopsies were taken from the antral and body mucosa, which were assessed for h. pylori colonisation and/or gastritis by an urease test (jatrox h. p.-test), microscopy after methylene-blue staining of a mucosal smear, specific culture and histology (stains: haematoxylin & eosin and warthin-starry). in addition, a simplified 13c-urea breat ...	1993	8379231
[clinico-pharmacologic considerations in helicobacter pylori eradication therapy].	problems and pitfalls of treatment regimens for eradication of helicobacter pylori (hp) are discussed from a clinical, pharmacological and microbiological point of view. problems concerning the physician who treats the patient consists mainly in inappropriate indication and suboptimal knowledge of drugs, doses and duration of therapy against hp. for successful triple therapy with complicated dosage protocols cooperation with and compliance by the patient is stressed. physicochemical, pharmacolog ...	1993	8379234