Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| impairment of na+,k(+)-atpase activity following enterotoxigenic campylobacter jejuni infection: changes in na+, cl- and 3-o-methyl-d-glucose transport in vitro, in rat ileum. | unidirectional fluxes of na+, cl- and 3-o-methyl-d-glucose (3-mg) were measured in vitro across campylobacter jejuni live culture-infected and control rat ileal short-circuited tissues by the ussing chamber technique. net secretion of na+ and enhanced secretion of cl- ions was observed in the infected animals (p < 0.001, n = 6) as compared to the net absorption of na+ and marginal secretion of cl- ions in the control animals. there was a significant decrease in the mucosal-to-serosal fluxes of 3 ... | 1994 | 7851745 |
| cryptolepis sanguinolenta activity against diarrhoeal bacteria. | cryptolepine is the main alkaloid of cryptolepis sanguinolenta (lindl.) schlechter, a plant used in traditional medicine in west africa. the minimal inhibitory concentrations (mics) of cryptolepine, ethanol and aqueous extracts of cryptolepis sanguinolenta root were determined for 65 strains of campylobacter jejuni, 41 strains of campylobacter coli isolated from sporadic cases of gastroenteritis in portugal and 86 strains of vibrio cholerae isolated from patients with enteric infections in angol ... | 1994 | 7853867 |
| detection of flagellar antigen of campylobacter jejuni and campylobacter coli in canine faeces with an enzyme-linked immunosorbent assay (elisa)--new prospects for diagnosis. | a new diagnostic procedure was developed to detect the flagellar antigen of campylobacter jejuni and campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. the assay detected all c. jejuni or c. coli infected specimens c ... | 1994 | 7975201 |
| metabolic activity of pathogenic bacteria during semicontinuous anaerobic digestion. | in natural environments such as anaerobic digesters, bacteria are frequently subjected to the stress of nutrient fluxes because of the continual changes in the flow of nutrients, and to survive, they must be capable of adapting readily to nutrient changes. in this study, the metabolic activities of escherichia coli, salmonella typhimurium, yersinia enterocolitica, listeria monocytogenes, and campylobacter jejuni were studied within culture bags (versapor-200 filters, 0.22-microns pore size) in l ... | 1994 | 7986040 |
| effect of trisodium phosphate on biofilm and planktonic cells of campylobacter jejuni, escherichia coli o157: h7, listeria monocytogenes and salmonella typhimurium. | trisodium phosphate (tsp) has been approved by the united states department of agriculture as a post-chill antimicrobial treatment for raw poultry. this study examines the effectiveness of tsp against planktonic (suspended) and biofilm (attached) cells of campylobacter jejuni, escherichia coli o157:h7, listeria monocytogenes and salmonella typhimurium at room temperature (rt) and 10 degrees c. at either temperature e. coli o157:h7 was the most sensitive to tsp treatments; 10(6) cfu/ml of plankto ... | 1994 | 7986678 |
| production and viability of coccoid forms of campylobacter jejuni. | studies were conducted into the formation and physiological state of coccoid cells of a strain of the human and animal pathogen campylobacter jejuni. it was found that growth phase and the presence of chloramphenicol did not affect the rate of shape transformation from spiral to coccoid, while nutrient limitation, aeration of the medium and the presence of free-radical scavengers had profound effects. coccoid cells were found to reduce the tetrazolium salts int (2-(p-iodophenyl)-3-(p-nitrophenyl ... | 1994 | 7989256 |
| [guillain-barré syndrome]. | sera from patients with guillain-barré syndrome (gbs) following campylobacter jejuni infection have autoantibody to gm1 ganglioside in the acute phase of the illness. there is a strong association between gbs and penner's serotype 19 (pen 19) of c. jejuni. the terminal structure of the bacterial lipopolysaccharide is identical to the terminal tetrasaccharide of gm1 ganglioside. anti-gm1 antibody inhibits motoneuron excitability. molecular mimicry between infectious agents and nerve tissue compon ... | 1994 | 7996693 |
| a survey of campylobacter jejuni in broilers from assignment to slaughter using dna-dna hybridization. | a survey of campylobacter jejuni in the cecal contents of broilers raised on a farm was carried out by the dna-dna hybridization method from the day of assignment to slaughter at about 1-week intervals. c. jejuni was detected in chickens as early as 1 week of age, and was widely detected at each week of age throughout the growing period. in addition, of 20 chickens tested just after assignment, 7 (35%) were c. jejuni positive. it is suggested that newly introduced chickens may have already been ... | 1994 | 7999894 |
| the immediate bactericidal effect of lactic acid on meat-borne pathogens. | the kinetics of the bactericidal effect of lactic acid decontamination (lad) on meat-borne pathogens (salmonella spp., campylobacter jejuni and listeria monocytogenes) was studied in an in-vitro model. the bactericidal effect was greatest on organisms in the lactic acid film that replaced the natural fluid on the meat surface during lad. a stepwise increase in ph from 2.6 to 3.5 and 4.0 progressively reduced the bactericidal effect of decontamination. for treatment with 2% lactic acid for 30-90 ... | 1994 | 8002475 |
| immunological properties and ganglioside recognitions by campylobacter jejuni-enterotoxin and cholera toxin. | the immunological properties of campylobacter jejuni enterotoxin (cjt) and cholera toxin (ct) were compared by enzyme-linked immunosorbent assay (elisa) and western blotting analysis with antiserum against each toxin. antibody against cjt recognized the 68, 54 and 43 kda polypeptides of cjt and the 11 kda subunit of ct, whereas antibody against ct recognized the 68 and 54 kda polypeptides of cjt and 11 kda subunit of ct. the immunological reactions between the heterogeneous combinations of toxin ... | 1994 | 8004056 |
| genetic, enzymatic, and pathogenic studies of the iron superoxide dismutase of campylobacter jejuni. | campylobacter jejuni is a microaerobic bacterium that produces an acute, self-limiting, watery or bloody diarrhea in humans. little is known about how c. jejuni causes disease or even what specific capabilities it requires for survival in vivo. the enzyme, superoxide dismutase (sod), which catalyzes the breakdown of superoxide radicals to hydrogen peroxide and dioxygen is one of the bacterial cell's major defense mechanisms against oxidative damage. a pcr-based search for sod genes in c. jejuni ... | 1994 | 8005660 |
| [bacteremia caused by quinolone-resistant campylobacter jejuni in a patient with hiv infection]. | 1994 | 8011729 | |
| inhibition of campylobacter jejuni colonization in chicks by defined competitive exclusion bacteria. | campylobacter enteritis in humans has been linked to consumption of chicken. reducing campylobacter jejuni colonization in chickens can potentially reduce campylobacter infections in humans. in this study, the reduction of c. jejuni colonization in chicks by oral administration of defined competitive exclusion (ce) cultures, 2.5% dietary carbohydrates, or ce cultures and carbohydrates was examined. prevention, elimination, or direct challenge of campylobacter infection was simulated by administe ... | 1994 | 8017915 |
| effects of helicobacter pylori on histamine and carbachol stimulated acid secretion by human parietal cells. | helicobacter pylori (h pylori) infection is associated with hypo, normal, and hypersecretory disorders of the gastric mucosa. pathophysiological pathways by which h pylori interacts with acid secretion are still unclear. the effects of h pylori on (14c) aminopyrine uptake by human parietal cells were examined as an indirect assay for acid secretion. isolated oxyntic glands were stimulated with submaximal concentrations of histamine or carbachol and incubated with sonicates of different h pylori ... | 1994 | 8020799 |
| cloning, nucleotide sequence and characterization of a gene encoding superoxide dismutase from campylobacter jejuni and campylobacter coli. | genes encoding superoxide dismutase (sod: ec 1.15.1.1) were cloned from campylobacter jejuni nctc 11351 and campylobacter coli ua585 by heterologous complementation of a sod-deficient escherichia coli mutant. deletion analysis of the cloned c. jejuni dna assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. the deduced gene product of 220 amino acids was 71% identical to the e. coli iron-containing sod and 60% identical to the e. coli manganese-containing s ... | 1994 | 8025686 |
| [serological study of campylobacter jejuni infections in patients with guillain-barré syndrome]. | the association between campylobacter jejuni infection and guillain-barré syndrome (gbs) was investigated serologically in 52 gbs patients. the specific antibody titers of immunoglobulin g class to c. jejuni were measured in the patient' sera using an enzyme-linked immunosorbent assay with acid-extracted antigen. the analysis showed that 31 (59.6%) of the 52 patients had evidences of recent c. jejuni infections in contrast to 16 (8.9%) of 180 healthy persons. the seropositive patients were analy ... | 1994 | 8027593 |
| inactivation of escherichia coli o157:h7, salmonellae, and campylobacter jejuni in raw ground beef by gamma irradiation. | raw ground beef patties inoculated with stationary-phase cells of escherichia coli o157:h7, salmonellae, or campylobacter jejuni were subjected to gamma irradiation (60co) treatment, with doses ranging from 0 to 2.52 kgy. the influence of two levels of fat (8 to 14% [low fat] and 27 to 28% [high fat]) and temperature (frozen [-17 to -15 degrees c] and refrigerated [3 to 5 degrees c]) on the inactivation of each pathogen by irradiation was investigated. in ascending order of irradiation resistanc ... | 1994 | 8031098 |
| [campylobacter jejuni and hiv infection]. | to evaluate the characteristics of the campylobacter jejuni infection in hiv positive patients. | 1994 | 7912110 |
| flagellin gene polymorphism analysis of campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping. | flagellin gene sequence polymorphisms were used to discriminate amongst 77 strains of campylobacter jejuni from sporadic and outbreak-associated human enteric infections, and from chickens, sheep and calves. the results were assessed in relation to lior biotyping and serotyping (penner somatic antigens). eight dna pcr-rflp patterns (genotypes) were identified by analysis of hinfi fragment length polymorphisms in flagellin gene (flaa) polymerase chain reaction (pcr) products. one genotype (f-1) w ... | 1994 | 7925661 |
| [toxic megacolon with colonic perforation complicating campylobacter jejuni ileo-colitis]. | campylobacter jejuni is an enteropathogen with invasive ability which may be responsible for acute colitis. we report the case of a 38-year-old woman who was hospitalized for severe acute colitis. stool specimen examination revealed campylobacter jejuni. a laparotomy was performed due to clinical deterioration and toxic megacolon. colic perforations were observed. the examination of the colectomy specimen revealed ulcerative pancolitis. this is the first published case of campylobacter jejuni in ... | 1994 | 7926445 |
| heat shock- and alkaline ph-induced proteins of campylobacter jejuni: characterization and immunological properties. | the protein response to physiological stress was characterized in campylobacter jejuni 81176 after exposure to heat and ph shock and following periods of recovery. immunoreactivities of major stress-related proteins were determined with anti-campylobacter immune rabbit serum and intestinal lavage fluid. distinct proteins with molecular masses ranging from 10 to 120 kda were induced and/or released by selective heat or ph treatments. the most notable responses were those of two proteins with appa ... | 1994 | 7927682 |
| mechanisms of quinolone resistance. | two mechanisms of resistance to fluoroquinolones are known: (i) alteration of the molecular target of quinolone action-dna gyrase, and (ii) reduction of the quinolone accumulation. mutations altering the n-terminus of the gyrase a subunit, especially those around residues ser83 and asp87, significantly reduce the susceptibilities towards all quinolones, while alterations of the gyrase b subunit are rarely found and are of minor importance. reduced drug accumulation is associated with alterations ... | 1994 | 7927834 |
| biodiversity of apidaecin-type peptide antibiotics. prospects of manipulating the antibacterial spectrum and combating acquired resistance. | insects have a unique repertoire of peptide antibiotics but, to date, prospects of clinical applications are not clear. apidaecin, a small peptide isolated from honeybees, inhibits viability of gram-negative bacteria; lethal activity is near immediate, independent of a conventional "lytic" mechanism, and involves stereoselective recognition of target molecules. here we report structural analysis of 14 naturally occurring apidaecin-type peptides and the existence of evolutionarily conserved ("con ... | 1994 | 7929322 |
| expression of a novel autoantibody defined by the vh3-15 gene in inflammatory bowel disease and campylobacter jejuni enterocolitis. | this study newly introduces anti-vh mabs to assess the role of clonal b cell activity in inflammatory bowel disease. immunohistochemistry of colonic biopsies in ulcerative colitis (uc) and crohn's disease (cd), but not unaffected individuals, demonstrated uniform staining of intravascular erythrocytes with bk2, a monoclonal specific for the vh3-15 ig heavy chain gene product. staining was caused by erythrocytes opsinized in vivo by anti-erythrocyte abs present in patient sera and by using the vh ... | 1994 | 7930592 |
| seroreactivity to campylobacter jejuni and gangliosides in patients with guillain-barré syndrome. | seroreactivities to campylobacter jejuni or campylobacter coli and to the gangliosides gm1 and gd1b were studied by quantitative and immunoglobulin class-specific elisas in 42 patients with recent onset of acute guillain-barré syndrome (gbs), in 39 patients with positive campylobacter serology but no neurologic disease, and in 52 healthy blood donors. gbs patients showed positive reactivities to c. jejuni in 57%, 26%, and 2% for igg, iga, and igm, respectively, while blood donors had correspondi ... | 1994 | 7930724 |
| development of a tissue culture assay system for campylobacter jejuni cytotoxin and the influence of culture conditions on cytotoxin production. | a tissue culture assay system to detect campylobacter jejuni cytotoxin was developed, and the effect of culture conditions on cytotoxin production was examined. broth-culture filtrates from clinical isolates were used. the expression of cytotoxicity was dependent on the presence of serum in the culture medium. although the titre of cytotoxin was low, the greatest cytotoxicity was revealed when newborn calf (ncs), adult bovine or goat serum was added to minimum essential medium. moderate or weak ... | 1994 | 7932615 |
| henoch-schönlein purpura associated with campylobacter jejuni enteritis. case report. | after an episode of enteritis caused by c. jejuni a 15-year-old boy developed schönlein-henoch purpura. because of continuous proteinuria a renal biopsy was performed. light microscopy revealed focal proliferative glomerulonephritis. iga was the dominant immunoglobulin. a causal relationship between enteritis caused by c. jejuni and schönlein-henoch purpura complicated with focal proliferative glomerulonephritis is suggested. | 1994 | 7939469 |
| [etiology, diagnosis and course of infectious diarrhea in the liestal canton hospital (5-year retrospective study)]. | between 1987 and 1991 219 patients (1.3% of all hospitalized patients) with acute infectious diarrhea were investigated retrospectively. 52% of the patients were hospitalized and 48% were outpatients. in 55% the inducing diarrhea microorganism could be identified. the most frequently detected pathogens were endemic salmonella sp. and campylobacter jejuni (65%). imported diarrheas (shigella sp. and parasites) were rare, as only 15% of the patients had a history of travel. all clostridium difficil ... | 1994 | 7939515 |
| clinical aspects of campylobacter jejuni infections in adults. | campylobacter jejuni is an almost ubiquitous, microaerophilic, gram-negative rod. outbreaks have been associated with drinking raw milk or contaminated water and eating poultry. campylobacter jejuni accounts for 3.2% to 6.1% of cases of diarrheal illness in the general population of the united states, and infected patients frequently present with abdominal pain and fever. less frequently, c jejuni is responsible for bacteremia, septic arthritis, septic abortion, and other extraintestinal infecti ... | 1994 | 7941533 |
| persistent, recurring diarrhea in a colony of orangutans (pongo pygmaeus) caused by multiple strains of campylobacter spp. | a colony of 10 orangutans (pongo pygmaeus) experienced persistent, recurring diarrhea caused by multiple infections with campylobacter jejuni and c. coli. infections appeared to have occurred through several mechanisms, including fecal-oral transmission between orangutans, and possibly transmission by houseflies contaminated with the organisms from nearby chicken feces. among the 14 fecal and environmental c. jejuni isolates, 4 different antibiotic susceptibility profiles were detected; there we ... | 1994 | 7942349 |
| [campylobacter gastroenteritis in patients with human immunodeficiency virus infection]. | campylobacter bacteria are frequent, and usually slight causes of diarrhea in a normal host while in an immunosuppressed host the diarrhea may lead to severe pictures. the aim of this study was to analyze the clinical features of gastroenteritis by campylobacter spp. in hospitalized seronegative patients and in those with hiv infection. | 1994 | 7948112 |
| [acute cholecystitis caused by campylobacter jejuni]. | 1994 | 7948125 | |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| [a patient with guillain-barré syndrome in association with campylobacter jejuni enteritis (pen19:lio7) in a patient with hla-b52 antigen]. | we report a 15-year-old boy with guillain-barré syndrome (gbs) in association with campylobacter jejuni enteritis. a neurologic examination revealed distal-dominant weakness and areflexia. compound muscle action potentials were markedly reduced in amplitude, but the decrease in motor conduction velocities was slight. c. jejuni was isolated from his stool culture and the serotype belonged to pen19:lio7. thin-layer chromatography-immunostaining showed that his serum igg reacted strongly with gm1 a ... | 1994 | 7955736 |
| severe campylobacter jejuni diarrhoea in a patient with hodgkin's disease. | 1994 | 7963346 | |
| [unusual forms of bacteremia due to campylobacter jejuni in patients with hiv infection]. | 1994 | 7967876 | |
| antimicrobial susceptibility and plasmid analysis of campylobacter jejuni isolated from diarrhoeal patients and healthy chickens in northern india. | seventy-five strains of campylobacter jejuni isolated from humans with diarrhoea (45 strains) and healthy chickens (30 strains) were tested for their susceptibility to different antimicrobial agents: ampicillin, tetracycline, erythromycin, gentamicin, kanamycin, furazolidine and quinolones (nalidixic acid, norfloxacin, ciprofloxacin). the frequencies of resistance to ampicillin and tetracycline were 16 and 9.3% respectively. two strains (2.7%) exhibited resistance to quinolones as mentioned. one ... | 1994 | 7751568 |
| reproduction of proliferative enteritis in hamsters with a pure culture of porcine ileal symbiont intracellularis. | hamsters, three weeks old, were inoculated orally with suspensions of intracellular bacteria, grown in tissue culture cells, iec-18, rat enterocytes. cells had been infected with suspensions of intracellular bacteria derived from the lesions of proliferative haemorrhagic enteropathy occurring naturally in two pigs 916/91 and 1482/89. infected cell lines containing each separate strain, 916/91 and 1482/89, were passaged one, two or five times and pure cultures of intracellular bacteria, identifie ... | 1994 | 7801512 |
| experimental colonization of mice with campylobacter jejuni. | the ability of one human and two chicken strains of campylobacter jejuni to colonise and survive in three different strains of laboratory mice (nmri, cba and c57-black) was studied. mice were inoculated orally with campylobacter jejuni and faeces samples were cultured at regular intervals during the following months. the length of colonisation of mice differed between mouse strains but also between campylobacter strains. the mouse strain c57-black was not colonised with c. jejuni to the same deg ... | 1994 | 7801521 |
| guillain-barré syndrome. | guillain-barré syndrome has now become recognized as a clinical syndrome that may be due to several pathological entities, consisting of an acute inflammatory demyelinating polyradiculoneuropathy as well as an acute motor axonal neuropathy. campylobacter jejuni infection is a common preceding event and, together with anti-ganglioside gm1 antibodies, is associated with axonal damage and a poor outcome. the mechanism by which such antibodies damage axons is not clear. the miller fisher syndrome is ... | 1994 | 7804457 |
| development of resistance to macrolide antibiotics in an aids patient treated with clarithromycin for campylobacter jejuni diarrhea. | in an aids patient with diarrhea, identical isolates of campylobacter jejuni susceptible and, later, resistant to macrolide antibiotics were isolated from feces before and after treatment with clarithromycin. results of rrna gene restriction analysis and serotyping suggest that development of resistance rather than simultaneous infection with a susceptible and a resistant strain was responsible for this phenomenon. this is the first report of in vivo development of resistance by campylobacter je ... | 1994 | 7805694 |
| [beta-lactamases of campylobacter spp. and their use as epidemiologic markers]. | the tests of 64 clinical strains of campylobacter jejuni and 69 strains isolated from hens revealed beta-lactamases in 96.99 per cent of the cultures. the detected enzymes more frequently splitted oxacillin and ampicillin among 5 betalactams used as the substrates (ampicillin, azlocillin, oxacillin, cefoperazon and cefotaxime). on the whole 24 spectra of the beta-lactamase activity with the evident domination of the amazoxcfpctx spectrum (29.69 per cent) were detected in both the clinical strain ... | 1994 | 7695448 |
| adhesion activity of campylobacter jejuni for intestinal epithelial cells and mucus and erythrocytes. | live and formalin- or heat (100 degrees c, 30 min)-treated cells of campylobacter jejuni strains y6878 and y6817, and the supernatant of the heat-treated bacterial suspension had agglutinating activity for the intestinal epithelial cells of japanese quails and human (int407). among the erythrocytes derived from 7 animal species (human, horses, sheep, rabbits, mice, chickens, and quails), only rabbit erythrocytes were agglutinated by live and glutaraldehyde-, formalin-, or heat-treated cells of 1 ... | 1994 | 7696403 |
| discrimination by means of pulsed-field gel electrophoresis between strains of campylobacter jejuni lior type 4 derived from sporadic cases and from outbreaks of infection. | the cleavage patterns of the genomic dna in 42 strains of campylobacter jejuni lior type 4, obtained from sporadic cases and from outbreaks of infection were analysed by means of pulsed-field gel electrophoresis (pfge). the cleavage of dna with smai and sali restriction enzymes showed 16 distinct fragment patterns in 20 sporadic isolates, indicating that they were heterogeneous. on the other hand, the patterns of 22 isolates derived from two outbreaks showed the same unique restriction patterns, ... | 1994 | 7806881 |
| oxygen tolerance estimates in campylobacter species depend on the testing medium. | oxygen tolerance of the microaerophile campylobacter jejuni subsp. jejuni varied with different brands of complex media which were used for plating the dilute cell suspensions. the tryptone component was one factor. with some tryptones growth occurred at 21% oxygen whereas with others there was no growth at oxygen levels of 15% or higher. a chemically-defined, agar-solidified plating medium was used to estimate the oxygen tolerance of camp. jejuni subsp. jejuni, camp. coli and camp. fetus subsp. ... | 1994 | 7822225 |
| identification of campylobacter jejuni, campylobacter coli and campylobacter lari by the nucleic acid amplification system nasbar. | nasbar, an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of campylobacter jejuni, camp. coli and camp. lari. a set of primers and a probe were chosen from the 16s rrna sequence of campylobacter. the probe was hybridized in solution with the amplified nucleic acids of 12 campylobacter species and nine other gram-negative bacteria. the probe was shown to hybridize specifically to the amplified single-stranded rna of camp. jejuni, camp. coli and ... | 1994 | 7822229 |
| a pcr assay for the detection of campylobacter jejuni and campylobacter coli in water. | a pcr assay has been developed for the detection of campylobacter jejuni and camp. coli in water samples. the sample is filtered through a membrane which is subjected to sonication to release the impacted cells. after removal of the filter from the cell suspension and a freeze/thaw cell lysis step, a semi-nested pcr is carried out on the filtrate using the primers cf02, cf03 and cf04 (camp. jejuni flaa and flab gene sequences). incorporation of a sonication stage allows removal of the filter mem ... | 1994 | 7765441 |
| inhibition of dnase activity in pfge analysis of dna from campylobacter jejuni. | dnase-positive strains of campylobacter jejuni degrade their chromosomal dna during standard preparative procedures before pulsed-field gel electrophoresis (pfge). a simple method for inactivation of this dnase activity is described. formaldehyde fixation of the bacterial cells resulted in the preservation of the dna in a state suitable for restriction digestion and subsequent electrophoretic analysis. | 1994 | 7765449 |
| ultrastructure of the bacteria campylobacter and helicobacter: implications for the phylogeny of mammal gastric bacteria. | negative staining, thin sections, and surface replica methods were used to produce ultrastructural descriptions of curved-shaped, flagelated bacteria campylobacter jejuni, helicobacter pylori, and h. muridarum, the last one from the ileon of mice. the former has nude monotrichous flagella, the others have the sheated lobotrichous type. h. muridarum presents 10-12 periplasmic fibers. a cladogram of some gastric bacteria, based on the evolutionary history of the mammal hosts, hypothesizes that (1) ... | 1994 | 7701086 |
| [epidemiology and etiology of infectious diarrheas. the case of mexico]. | gastrointestinal infections represent a health problem. it is estimated that 1647 million cases of diarrhea and 3.2 million deaths due to this cause occur among children less than five years of age per year. those belonging to this age group have 15 times more risk of dying because of diarrhea. cases of liquid acute diarrhea with blood represent 80% of cases, diarrhea with blood represent 10%. most frequent causes of liquid diarrhea are enterotoxigenic escherichia coli and rotaviruses and most f ... | 1994 | 7701141 |
| occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples. | twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ... | 1994 | 7703010 |
| detection of the coccoid form of campylobacter jejuni in chicken products with the use of the polymerase chain reaction. | detection of the coccoid form of campylobacter jejuni with the use of the polymerase chain reaction (pcr) was examined. coccoid cells of this pathogen, formed at different temperatures, showed different detection characteristics in the pcr. for spirals and cocci formed at 4 degrees c and 12 degrees c, the detection limit was about 2 x 10(3) cells/pcr. however, for detection of coccoid cells formed at 25 degrees c and 37 degrees c, at least 2 x 10(4) cells per pcr were needed. pcr was also perfor ... | 1994 | 7703020 |
| isolation of motile and non-motile insertional mutants of campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells. | a method of insertional mutagenesis for naturally transformable organisms has been adapted from haemophilus influenzae and applied to the study of the pathogenesis of campylobacter jejuni. a series of kanamycin-resistant insertional mutants of c. jejuni 81-176 has been generated and screened for loss of ability to invade int407 cells. eight noninvasive mutants were identified which showed 18-200-fold reductions in the level of invasion compared with the parent. three of these eight show defects ... | 1994 | 7715450 |
| campylobacter jejuni infection and the guillain-barre syndrome: mechanisms and implications. | 1994 | 7727903 | |
| penner's serotype 4 of campylobacter jejuni has a lipopolysaccharide that bears a gm1 ganglioside epitope as well as one that bears a gd1 a epitope. | the carbohydrate structures of lipopolysaccharides (lpss) of campylobacter jejuni strains belonging to penner's serotypes (pen) 1, 2, 4, 19, 23, and 36 were studied by thin-layer chromatography and immunostaining with several monoclonal antiganglioside antibodies. anti-gm1 and anti-gd1a antibodies reacted with the lpss of pen 1, 4, and 19. aspinall et al. (g. o. aspinall, a. g. mcdonald, t. s. raju, h. pang, a. p. moran, and j. l. penner. eur. j. biochem. 213:1017-1027, 1993) recently reported t ... | 1994 | 7513313 |
| molecular mimicry between gq1b ganglioside and lipopolysaccharides of campylobacter jejuni isolated from patients with fisher's syndrome. | we isolated campylobacter jejuni from 2 patients with fisher's syndrome subsequent to enteritis. crude lipopolysaccharide fractions were extracted from the bacteria and separated by thin-layer chromatography. monoclonal antibodies to gq1b ganglioside (gmr13 and 7f5) reacted with both lipopolysaccharide fractions, indicating that the lipopolysaccharides bear the gq1b epitope. this is the first report of molecular mimicry between neural tissue components and the antecedent infectious agents of fis ... | 1994 | 7526777 |
| isotype, specificity, and kinetics of systemic and mucosal antibodies to campylobacter jejuni antigens, including flagellin, during experimental oral infections of chickens. | the immune response of chickens to campylobacter jejuni infection was studied as a step in the search for vaccine candidates. one-day-old chicks orally challenged with c. jejuni strain 81116 showed significant increases in specific igg, iga, and igm circulating antibodies, as detected by enzyme-linked immunosorbent assay (elisa). these levels peaked at 9, 5, and 7 weeks postinfection, respectively. maternal igg antibodies were also detected over the first 2 weeks. specific mucosal igg and iga an ... | 1994 | 7526839 |
| isolation and characterization of the flagellar hook of campylobacter jejuni. | a method for purification of the flagellar hook of campylobacter jejuni is described. the hook was shown to be composed of a subunit protein, which has a molecular mass of 92,000 and an isoelectric point of pi 4.8. a monoclonal antibody and a polyvalent antiserum was raised against the purified flagellar hook of c. jejuni. immuno-electronmicroscopy revealed that the epitope recognized by the monoclonal antibody is surface-located. however, this antibody reacted only with the hook of the immuniza ... | 1994 | 7527365 |
| identification and characterization of an intervening sequence within the 23s ribosomal rna genes of campylobacter jejuni. | campylobacter jejuni is a significant cause of bacterial enteritis in humans. three of seven c. jejuni isolates examined were found to contain fragmented 23s rrna. the occurrence of fragmented 23s rrna correlated with the presence of an intervening sequence (ivs) within the 23s rrna genes. the ivs is 157 nucleotides in length and replaces an eight nucleotide sequence in the 23s rrna genes of c. jejuni isolates that contain intact 23s rrna. the two ends of the ivs share 31 bases of complementarit ... | 1994 | 7530317 |
| lipopolysaccharides of campylobacter jejuni serotype o:19: structures of o antigen chains from the serostrain and two bacterial isolates from patients with the guillain-barré syndrome. | an o antigenic polysaccharide was liberated from the lipopolysaccharide of high m(r) from campylobacter jejuni serotype o:19 by acetic acid hydrolysis of the ketosidic linkage to lipid a. the structure of the polysaccharide was established in several one- and two-dimensional 1h and 13c nmr experiments, fast atom bombardment mass spectrometry and methylation linkage analysis of the permethylated glycan and its degradation products. it is concluded that the glycan is a derivative of hyaluronic aci ... | 1994 | 7506928 |
| serum antibody response to the superficial and released components of helicobacter pylori. | superficial and released components were extracted from six selected helicobacter pylori strains. the protein and antigenic profiles of these extracts were representative of the profiles found most frequently among the clinical strains and included major peptidic fractions at 19, 23.5, 57, 68, 76, 118, and 132 kda and major antigens at 68, 57, and 23.5 kda. immuno-cross-reactions were seen with a hyperimmune rabbit serum to campylobacter fetus but not with sera to campylobacter jejuni or salmone ... | 1994 | 7496968 |
| first isolation of campylobacter jejuni from the vaginal discharge of three bitches after abortion in south africa. | campylobacter jejuni was isolated in pure culture from the vaginal discharge from three german shepherd bitches after late-pregnancy abortions. the main clinical sign occurring in the bitches was a profuse and odourless haemorrhagic vaginal discharge. | 1994 | 7596570 |
| ribosomal rna patterns identify additional strains of campylobacter jejuni and c. coli among isolates serotyped by heat-stable and heat-labile antigens. | heat-stable (hs, o-antigen) and heat-labile (hl) serotyping are the most common methods used to type campylobacter jejuni and c. coli for epidemiologic purposes. in this study, we conducted rrna analysis to differentiate strains of c. jejuni and c. coli that had been serotyped by use of the passive hemagglutination (heat-stable) and slide agglutination (heat-labile) methods. ribotyping of isolates within hs and hl serotypes revealed further discrimination of strains. four ribotypes were identifi ... | 1994 | 7534848 |
| [ecological and food safety considerations about products of vegetable origin]. | media have paid much attention in recent years to emerging microbiological problems in foods of plant origin. the potential for contamination of fruits and vegetables is high because of the wide variety of conditions to which produce is exposed during growth, harvest, processing and distribution. these considerations acquire great significance in the current scenario of the new processing techniques that offer attributes of convenience and fresh-likeness in response to changes in consumption pat ... | 1994 | 8984963 |
| bacterial enteric pathogens in uncooked foods in thai markets. | in developing countries, the morbidity and mortality rates of gastrointestinal tract infections from food borne bacteria have been difficult to establish. most studies have only been able to gather data prospectively from isolated geographic sources, rather than from large point-source epidemics. this study investigates the types of bacterial enteric pathogens found in food that was collected in a community in western thailand, where sporadic cases of hemolytic uremia syndrome and cholera have b ... | 1994 | 9815313 |
| ph and acidity in lactic-fermenting cereal gruels: effects on viability of enteropathogenic microorganisms. | survival of bacillus cereus, campylobacter jejuni, enterotoxigenic escherichia coli, salmonella typhimurium and shigella flexneri during lactic acid fermentation of cereal gruels prepared from low-tannin (white) and high-tannin (red) sorghum varieties was studied. a previously fermented gruel (starter culture, sc) recycled daily or stored for 7, 14 or 28 days, germinated cereal flour (power flour, pf), or a combination of pf and sc (pf+sc) were used as starters. at 24 h, the ph of all gruels wit ... | 1994 | 24421191 |
| guillain-barré syndrome with severe persistent disability: relationship to hyperacute guillain-barré syndrome. | guillain-barré syndrome (gbs) is a heterogeneous condition with a variable prognosis. we studied nine patients who were unable to walk unaided 12 months after the onset of their illness to discover whether they belonged to a more homogeneous subgroup. sis of the nine patients had symptoms of gastroenteritis shortly before the onset of their neuropathic symptoms and a hyperacute onset of weakness so that they were bed-bound within 24 h. these patients had predominantly motor rather than sensory i ... | 1994 | 24283425 |
| changes in cell morphology and polyamine composition during growth of campylobacter jejuni. | resting cells of campylobacter jejuni were spherical whereas growing cells were mainly spiral. content of cadaverine increased with the decrease in spherical forms prior to growth commencing but production of spermidine increased in early log phase. cadaverine and spermidine are possibly involved in changes in cell morphology and growth, respectively. | 1994 | 24421028 |
| cloning and expression of the erythromycin resistance determinant from campylobacter jejuni in escherichia coli. | the erythromycin resistance gene (em(r)) from campylobacter jejuni aba94 plasmid dna was cloned into the puc18 vector and then expressed in escherichia coli. the location of the em(r) determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb ecori fragment. this fragment then hybridized to the 78-kb plasmid dna but not to the 3.3-or 12.6-kb plasmid dna of campylobacter jejuni aba94. em(r) in campylobacter jejuni is therefore probably plasmid-mediated. | 1995 | 24415023 |
| epidemiologic observations on diarrhea developing in u.s. and mexican students living in guadalajara, mexico. | background: a previous study suggested that u.s. students who lived in mexico for 1 year had a risk of diarrhea intermediate between the rate for newly arrived u.s. students and mexican students; however, the study was not controlled for changes of risky behavior over time. methods: an analysis of acute diarrhea occurring among u.s. and mexican student groups living in guadalajara, mexico was conducted to explore the association of diarrhea developing during selected 28-day periods with length o ... | 1995 | 9815352 |
| enrichment medium for isolation of campylobacter jejuni-campylobacter coli. | a kaseini-broth enrichment medium (kbem) for the isolation improvement of campylobacter jejuni-campylobacter coli from stool samples is described. isolation of campylobacters from stool specimens by direct inoculation onto solid selective medium was compared with isolation after twenty-four hours enrichment at 37 degrees c in kbem, followed by subculture onto the same solid selective medium. of 156 examined stool samples from diarrhoeal children positive results were obtained from 17 patients al ... | 1995 | 9524671 |
| severe illness in african children with diarrhoea: implications for case management strategies. | to identify clinical disorders associated with severe illness in african children with diarrhoea, we studied a group of under-5-year-olds with diarrhoea who had been brought to a large public hospital in central cote d'ivoire. the general condition of children with diarrhoea was assessed and classified according to criteria recommended by who, and then used as a nonspecific indicator of severity. of the 264 children with diarrhoea who were enrolled in the study, 196 had nonsevere illness and 68 ... | 1995 | 8907771 |
| invasive and enterotoxic properties in campylobacter jejuni and campylobacter coli strains isolated from humans and animals. | invasive properties of 15 strains of thermotolerant campylobacter (12 c. jejuni and 3 c. coli) were studied using hela cells cultures. in four of them (3 c. jejuni and 1 c. coli), randomly selected, intestinal perfusion experiments were conducted in order to asses enterotoxigenicity. all strains were able to invade hela cells. the number of invaded hela cells ranged from 3 to 46%. in addition to their invasive properties, the 4 strains used in perfusion experiments were able to induce either a n ... | 1995 | 9251750 |
| safety and immunogenicity of a prototype oral whole-cell killed campylobacter vaccine administered with a mucosal adjuvant in non-human primates. | the safety and immunogenicity of two prototype oral campylobacter killed whole-cell (cwc) vaccines were tested in rhesus monkeys. animals were immunized with a primary two-dose series (days 0 and 14) of vaccine consisting of cwc (10(10) particles/dose) given alone or in combination with 0.5-1000 micrograms of the heat-labile enterotoxin of escherichia coli as an oral adjuvant (oa). a booster vaccination, 4 weeks after primary immunization, was given to animals receiving cwc alone or supplemented ... | 1995 | 7539199 |
| expression of adhesion molecules on human granulocytes after stimulation with helicobacter pylori membrane proteins: comparison with membrane proteins from other bacteria. | type b gastritis in its active form is characterized by a dense infiltration of the lamina propria with granulocytes. since the bacterium helicobacter pylori does not invade the epithelial barrier, a signaling pathway chemoattractive for granulocytes must exist across this mucosal boarder. one possible mechanism tested was whether granulocytes are directly activated by water-soluble membrane proteins (wsp) from h. pylori. these findings were compared with the effects of wsp from other bacteria ( ... | 1995 | 7540595 |
| molecular subtyping scheme for serotypes hs1 and hs4 of campylobacter jejuni. | we describe a molecular subtyping scheme for two principal o (heat-stable [hs]) serotypes of campylobacter jejuni, hs1 and the hs4 complex. a 16s rrna gene-specific probe confirmed that almost all the c. jejuni strains had three copies of this gene, and strains could be assigned with complete typeability to 1 of 16 combined (pst1 and haeiii) 16s ribotypes. macrorestriction profiles (mrps) consisting of up to 10 smai fragments from approximately 40 to approximately 480 kbp were resolved by pulsed ... | 1995 | 7540628 |
| conformational analysis of the campylobacter jejuni porin. | the major outer membrane protein (momp) of campylobacter jejuni was purified to homogeneity by selective solubilization and fast protein liquid chromatography. the amino acid composition of the momp indicates the presence of cysteine residues. the amino-terminal sequence, determined over 31 residues, shows no significant homology with any other porin from gram-negative bacteria except in a discrete region. immunocross-reactivity between escherichia coli ompc and the momp was analyzed, and a comm ... | 1995 | 7543469 |
| subclass of igg antibody to gm1 epitope-bearing lipopolysaccharide of campylobacter jejuni in patients with guillain-barré syndrome. | sera of patients who develop guillain-barré syndrome (gbs) subsequent to campylobacter jejuni enteritis frequently have igg anti-gm1 antibody. lipopolysaccharide (lps) of c. jejuni isolated from a gbs patient has a gm1 ganglioside-like structure. igg subclass distribution of the anti-gm1 antibody in gbs patients is mainly restricted to igg1 and igg3. since igg antibodies to bacterial polysaccharide generally are restricted to igg2 subclass, some investigators have assumed that either the general ... | 1995 | 7543907 |
| chemical structures of the core region of campylobacter jejuni o:3 lipopolysaccharide and an associated polysaccharide. | the complete structure for the core oligosaccharide region of the water-insoluble low-m(r) lipopolysaccharide of campylobacter jejuni serotype o:3 from phenol/water extraction of bacterial cells was assigned through studies on derivatives of the liberated oligosaccharide. structure determinations were performed using 1h-nmr and 31p-nmr spectroscopies, methylation analysis supported by fast-atom-bombardment mass spectrometry, and smith degradation experiments. it was concluded that the complete c ... | 1995 | 7544281 |
| ganglioside-like epitopes of lipopolysaccharides from campylobacter jejuni (pen 19) in three isolates from patients with guillain-barré syndrome. | sera from patients with guillain-barré syndrome (gbs) frequently have anti-gm1 antibody. we earlier showed that an lipopolysaccharides (lps) from campylobacter jejuni (pen 19) isolated from a gbs patient has a gm1 ganglioside-like structure. aspinall et al. (biochemistry, 61 (1994) 335-337) reported that oh 4382 has an lps that bears a cd3 ganglioside-like structure and that oh 4384 has an lps that bears a gt1a-like structure; both strains were isolated from patients with gbs. they also suggeste ... | 1995 | 7544402 |
| invasion and viability of campylobacter jejuni in experimentally contaminated japanese quails' eggs. | in japanese quail eggs experimentally immersed in a suspension of campylobacter jejuni from human patients (y6817, y6878), strain y6817 was recovered from 15 (18.8%) eggs among 80 between 0.5 hr and 72 hr after immersion. invasion of the eggs by strain y6878 was also seen in 10 (12.5%) of 80 eggs between 0.5 hr and 24 hr after immersion. when egg yolk was inoculated with both strains and held at 4 degrees c, the organisms were detectable for a longer time than the ones kept at 20 degrees c. on t ... | 1995 | 7548430 |
| detection and identification of campylobacter coli and campylobacter jejuni by two-step polymerase chain reaction. | flagellin gene was used as target sequence to detect and distinguish c. coli and c. jejuni by a "nested pcr" technique. the method shows a high level of sensitivity and specificity. application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms. | 1995 | 7552696 |
| comparison of preservation media for storage of stool samples. | transportation of clinical samples and long-term recoverability of pathogens are critical to epidemiological studies, particularly when conditions do not permit immediate processing. this study confirms that cary-blair medium (cb) is suitable for the preservation of salmonella and shigella isolates for more than 2 weeks at 25, 4, or -70 degrees c. campylobacter jejuni was not recovered after 2 days of storage in cb at 25 degrees c when an inoculum of 12 x 10(8) cells per ml was used. lower tempe ... | 1995 | 7559972 |
| high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance. | campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ... | 1995 | 7561196 |
| cytotoxin detection in campylobacter jejuni strains of human and animal origin with three tissue culture assay systems. | cytotoxin (ctx) production in 34 human and 22 animal strains of campylobacter jejuni isolated in japan and other countries was studied by three assay systems described previously. furthermore, cholera-like enterotoxin production by these strains was tested by reversed passive latex agglutination (rpla). ctx titres in the fetal calf serum (fcs) and newborn calf serum (ncs) assays were relatively lower, with a maximum of 4 and 8, respectively, than the maximum of 128 for the serum-free culture (sf ... | 1995 | 7563000 |
| dna diversity among isolates of campylobacter jejuni detected by pcr-based rapd fingerprinting. | a pcr-based randomly amplified polymorphic dna method was used to amplify campylobacter jejuni dna using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum. the method was able to detect the heterogeneity of amplified dna from human, chicken and turkey sources and can be used as a tool to study the epidemiology of campylobacter jejuni infection. | 1995 | 7571378 |
| analysis of flagellin gene expression in flagellar phase variants of campylobacter jejuni 81116. | flagella production in campylobacter jejuni 81116 is subject to phase variation; the bacterium is able to switch its flagellum synthesis, and thereby its motility, on and off. under standard laboratory growth conditions flagellar phase variants can be maintained as stable, pure cultures. we found conditions that efficiently induced a phase shift in vitro. the flaa gene but not the flab gene is subject to the on and off switch. minor amounts of flab are still present in aflagellate cells. we prev ... | 1995 | 7574555 |
| survival of campylobacter jejuni in foods and comparison with a predictive model. | campylobacter jejuni was inoculated into a range of raw and cooked foods and survival determined during storage at 2 degrees, 10 degrees and 20 degrees c for up to 56 d. to facilitate easy enumeration, two antibiotic-resistant strains of camp. jejuni, which had similar survival characteristics to the parent strain, were used. campylobacter jejuni survived for longer at lower temperatures in all foods and inactivation was most rapid in pâté. there was generally good agreement between the survival ... | 1995 | 7576507 |
| effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum. | we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ... | 1995 | 7581046 |
| physical and genetic map of the genome of campylobacter upsaliensis. | a physical map of campylobacter upsaliensis atcc 43954 was constructed from dna fragments generated by sali (5' g/tcgac), nari (5' gg/cgcc) and bsshii (5' g/cgcgc) restriction digests separated using pulsed-field gel electrophoresis. the size of the c. upsaliensis genome was approximately 2000 kb, providing evidence of the largest campylobacter genome sized to date. twenty-one fragments created from these restriction digests were assembled into a physical map using a combination of complementary ... | 1995 | 7582001 |
| an isoleucyl-trna synthetase gene from campylobacter jejuni. | a complete isoleucyl-trna synthetase gene (iles) of campylobacter jejuni was isolated from a c. jejuni tgh9011 genomic dna library constructed in pbluescript. the complete coding sequence, flanking regions and transcription start point were determined. the deduced isoleucyl-trna synthetase (ilers) had 917 amino acids with a molecular mass of 105,399 da, which was consistent with the observed size of 105 kda in escherichia coli maxicells. the iles gene was mapped onto the physical map of the c. j ... | 1995 | 7582016 |
| detection of genomic variability among isolates of campylobacter jejuni from chickens by crossed-field gel electrophoresis. | digestion with sal i facilitated the subclassification of 41 strains of campylobacter jejuni into seven types, and digestion with sma i enabled subclassification into twelve types. sma i was potentially more useful for the detection of variability among the 41 strains, but both restriction enzymes seemed to be potentially useful for detecting variability among crossed-field gel electrophoresis profiles of the strains. the results clearly demonstrated that c. jejuni strains from different sources ... | 1995 | 7587375 |
| comparison of two selective media and a membrane filter technique for isolation of campylobacter species from diarrhoeal stools. | diarrhoeal stool specimens from 415 patients were examined for campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (ccda), skirrow medium and columbia blood agar overlaid with a 0.65 micron pore size membrane filter. forty-eight campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were campylobacter jejuni (91.7%), three were campylobacter coli (6.3%) and one was campylobacter hyointestinalis (2.0%). the percentages of campylobacter-positive specime ... | 1995 | 7588831 |
| comparison of pfge, ribotyping and phage-typing in the epidemiological analysis of campylobacter jejuni serotype hs2 infections. | in this study we have evaluated the ability of three typing methods, pulsed field gel electrophoresis (pfge), phage-typing and ribotyping, to discriminate not only between strains of differing serotypes but also between strains within a single serotype, heat stable serotype 2 (hs2). forty-five isolates derived from cases of campylobacter enteritis occurring in the cardiff area were examined. these included 18, mostly hs2, strains associated with an outbreak. the typing results for these and a fu ... | 1995 | 7589261 |
| complete sequences and organization of the rrna operon from campylobacter jejuni tgh9011 (atcc43431). | the rrna ribosomal rna (rrna) operon of campylobacter jejuni (cj) tgh9011 (atcc43431) was cloned and sequenced to completion. rrnas were then characterized by primer extension and s1 nuclease mapping analysis. the secondary structure models of cj 16s and 23s rrnas were constructed, and the models were compared to the corresponding models from other eubacterial rrna. the analysis presented a typical 5'-promoter-16s-trnas-23s-5s-terminator-3' prokaryotic rrna operon structure. however, an unusual ... | 1995 | 7590296 |
| cloning and transcription regulation of the ferric uptake regulatory gene of campylobacter jejuni tgh9011. | a campylobacter jejuni (cj) tgh9011 (atcc 43431) gene homologous to the escherichia coli ferric uptake regulatory gene (fur) has been cloned and characterized. cj fur encodes a polypeptide consisting of 157 amino acids (aa) (18.1 kda). the 5'-flanking region of the cj fur gene contains two putative catabolite activator protein (cap)-binding sequences and four fur boxes or fur-binding sequences (fbs), implicating camp and autogenous regulation respectively. a major and a minor transcription start ... | 1995 | 7590316 |
| campylobacter jejuni motility and invasion of caco-2 cells. | we investigated the influence of motility on campylobacter jejuni binding and invasion of caco-2 cells. c. jejuni was motile in soft agar at basic (ph 8.5) and neutral ph values representative of the intestinal environment. however, c. jejuni was immobilized at ph 5.0. the inability of c. jejuni to swarm on soft agar at ph 5.0 was not related to flagellar depolymerization or loss of viability. in tissue culture medium, c. jejuni displayed typical periods of straight swimming punctuated by tumbli ... | 1995 | 7591061 |
| acute cholecystitis caused by campylobacter jejuni. | an 83-year-old man with acute cholecystitis caused by campylobacter jejuni is described. the patient was cured after undergoing cholecystectomy and intravenous ofloxacin therapy. | 1995 | 7591706 |
| cytotoxin production by campylobacter jejuni isolated from turkish patients with diarrhoea. | 1995 | 7594315 | |
| electrophysiological studies in guillain-barré syndrome: correlation with antibodies to gm1, gd1b and campylobacter jejuni. | a retrospective study of 50 patients with guillain-barré syndrome (gbs) correlated analysis of serial motor nerve conduction studies with the presence of antibodies to campylobacter jejuni, gm1 and gd1b, determined by elisa. gbs patients with antibodies to c. jejuni (n = 8), gm1 (n = 4), or gd1b (n = 4) showed electrophysiological features suggestive of demyelination with prolonged distal motor latencies and temporal dispersion/conduction block similar to gbs patients without these specific anti ... | 1995 | 7595678 |