Publications

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performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for iga.the performance of an organic thin film photodiode (opd), fabricated from a hetero-junction comprised of two layers of c(60) and a phthalocyanine-cu(ii) complex was evaluated by detecting the chemiluminescence generated from the reaction of luminol with horseradish peroxidase in the presence of h(2)o(2), and the fluorescence from resorufin, as an optical detector. the photocurrent of the opd was linear with respect to the power of light from a commercial led. the sensitivity of the opd was suffi ...201222817940
signal amplification of microarray-based immunoassay by optimization of nanoliposome formulations.the use of microarray-based immunoassay is often limited by its sensitivity. to increase the sensitivities of such an immunoassay, liposome encapsulation was explored. two different liposome formations and several preparation methods were examined to optimize encapsulation and signal-enhancing efficacy for enzyme-linked immunosorbent assay (elisa) and antibody array. the signal amplification by liposome encapsulation was demonstrated through a detection for foodborne pathogenic listeria. in plat ...201222809874
programmed cell death in plants: protective effect of tetraphenylphosphonium and tetramethylrhodamine cations used as transmembrane quinone carriers.tetraphenylphosphonium (tpp(+)) and tetramethylrhodamine ethyl ester (tmre(+)) cations used as transmembrane carriers of ubiquinone (mitoq) and plastoquinone (skq, skqr) in mitochondria prevented at nanomolar concentrations the chitosan- or h(2)o(2)-induced destruction of the nucleus in epidermal cells of epidermis isolated from pea leaves. the protective effect of the cations was potentiated by palmitate. penetrating anions of tetraphenylboron (tb(-)) and phenyl dicarbaundecaborane also display ...201222809154
[effects of electromagnetic pulse exposure on the permeability of inner blood-retinal barrier model in vitro].to establish the inner blood-retinal barrier (brb) model in vitro by co-culturing rf/6a cells and c6 cells and to investigate the effects of emp (200 kv/m, 200 pulses) exposure on the permeability of the inner brb model in vitro.201222804884
a disposable electrochemical immunosensor based on carbon screen-printed electrodes for the detection of prostate specific antigen.a novel screen-printed electrode (spes) on sheets of vegetable parchment was prepared. the obtained spes were stable, convenient, inexpensive and suitable for large-area screen-printing. with these spes, we explored the fabrication of a novel, disposable and highly sensitive electro-analytical immunosensor using graphene nanosheets (gs) and horseradish peroxidase (hrp)-labeled signal antibody functionalized with gold nanoparticles (hrp-ab(2)/au nps). gs was used to increase the conductivity and ...201222770827
gold-fish: a new approach for the in situ detection of single microbial cells combining fluorescence and scanning electron microscopy.a novel fluorescence in situ hybridisation (fish) method is presented that allows the combination of epifluorescence and scanning electron microscopy (sem) to identify single microbial cells. first, the rrna of whole cells is hybridised with horseradish peroxidase-labelled oligonucleotide probes and this is followed by catalysed reporter deposition (card) of biotinylated tyramides. this facilitates an amplification of binding sites for streptavidin conjugates covalently labelled with both fluoro ...201222770611
wrapping tissues with a pre-established cage-like layer composed of living cells.we report a method for wrapping tissues with a pre-established cage-like layer composed of living cells. we encapsulated multicellular aggregates of human hepatoma hepg2 cells as a model of tissues such as pancreatic islets and hepatocyte spheroids in alginate-based hydrogel microcapsules and subsequently coated the microcapsule surface with a gelatin derivative through a horseradish peroxidase-catalyzed reaction. human aorta endothelial (hae) cells grew on the surface and formed a cell layer wi ...201222770525
disposable amperometric magneto-immunosensor for direct detection of tetracyclines antibiotics residues in milk.the preparation and performance of a disposable amperometric magneto-immunosensor, involving the use of a selective capture antibody immobilized on the surface of protein g-functionalized magnetic beads (protg-mbs) and screen-printed carbon electrodes (spces), for the specific detection and quantification of tetracyclines (tcs) residues in milk is reported. a direct competitive immunoassay using a tracer with horseradish peroxidase (hrp) for the enzymatic labeling was performed. the amperometric ...201222769033
recognition-driven layer-by-layer construction of multiprotein assemblies on surfaces: a biomolecular toolkit for building up chemoresponsive bioelectrochemical interfaces.the development of soft bioelectronic interfaces with accurate compositional and topological control of the supramolecular architecture attracts intense interest in the fast-growing field of bioelectronics and biosensing. the present study explores the recognition-driven layer-by-layer assembly of glycoenzymes onto electrode surfaces. the design of the multi-protein interfacial architecture is based on the multivalent supramolecular carbohydrate-lectin interactions between redox glycoproteins an ...201222766969
photooxidation of amplex red to resorufin: implications of exposing the amplex red assay to light.the amplex red assay, a fluorescent assay for the detection of h(2)o(2), relies on the reaction of h(2)o(2) and colorless, nonfluorescent amplex red with a 1:1 stoichiometry to form colored, fluorescent resorufin, catalyzed by horseradish peroxidase (hrp). we have found that resorufin is artifactually formed when amplex red is exposed to light. in the absence of h(2)o(2) and hrp, the absorption and fluorescence spectra of amplex red changed during exposure to ambient room light or instrumental e ...201222765927
amperometric bienzymatic biosensor for l-lactate analysis in wine and beer samples.a novel amperometric bienzymatic biosensor has been developed based on the incorporation of lactate oxidase (lox) and horseradish peroxidase (hrp) into a carbon nanotube/polysulfone membrane by the phase inversion technique onto screen-printed electrodes (spes). in order to improve the sensitivity and reduce the working potential, experimental conditions have been optimized and ferrocene has also been incorporated into the membrane as a redox mediator of the enzymatic reactions, which allows the ...201222763889
a novel glucose biosensor using bi-enzyme incorporated with peptide nanotubes.a novel amperometric glucose biosensor was developed using the bio-inspired peptide nanotube (pnt) as an encapsulation template for enzymes. horseradish peroxidase (hrp) was encapsulated by the pnt and glucose oxidase (go(x)) was co-immobilized with the pnt on a gold nanoparticle (aunp)-modified electrode. a binary sam of 3-mercaptopropionic acid (mpa) and 1-tetradecanethiol (tdt) was formed on the surface of the electrode to immobilize the pnt and go(x). the resulting electrode appeared to prov ...201222763144
improvement in determination of isothiocyanates using high-temperature reversed-phase hplc.the largely adopted reversed-phase hplc analysis of the molecular species of isothiocyanates (itcs) was performed and showed losses during the chromatographic run with eight itcs. these losses, which obviously impact the accuracy of quantitative determinations, were due to precipitation in the chromatographic system. at 22°c, they ranged from 5.4% for sulforaphane (sfn) to 11.0% for benzyl-itc when itcs were injected at 80 μg ml(-1) , but they were up to three times higher at 1 mg ml(-1) reachin ...201222752995
pp131. regulation of heme oxygenase/carbon monoxide (ho/co) and l-arginine/nitric oxide (no) pathways by human chorionic gonadotropin (hcg) in human fetal endothelium.ho/co and l-arginine/no pathways are critical regulators of vascular function in early human pregnancy. ho is the rate-limiting enzyme in heme catabolism leading to the formation of biliverdin, which is subsequently reduced to bilirubin by biliverdin reductase, carbon monoxide, and free iron. ho-1, the inducible isoform of the enzyme, is essential to promote placental function and fetal development (1). ho-1 is also required to maintain nitric oxide synthesis (2). human chorionic gonadotropin (h ...201226105453
gold nanoparticle-enzyme conjugates based fret for highly sensitive determination of hydrogen peroxide, glucose and uric acid using tyramide reaction.in this paper, we report a new strategy for highly sensitive determination of hydrogen peroxide, glucose and uric acid based on fluorescence resonance energy transfer (fret) using gold nanoparticles (aunps) as energy acceptors. the principle is based on highly sensitive reaction of tetramethyl rhodamine (tmr) labeled tyramide and hydrogen peroxide catalysed by horseradish peroxidase (hrp), and the fluorescence spectrum of tmr (ex(max) 575 nm) partially overlaps with the visible absorption bands ...201222745932
a fluorescently labeled dendronized polymer-enzyme conjugate carrying multiple copies of two different types of active enzymes.a hybrid structure of a synthetic dendronized polymer, two different types of enzymes (superoxide dismutase and horseradish peroxidase), and a fluorescent dye (fluorescein) was synthesized. thereby, a single polymer chain carried multiple copies of the two enzymes and the fluorescein. the entire attachment chemistry is based on uv/vis-quantifiable bis-aryl hydrazone bond formation that allows direct quantification of bound molecules: 60 superoxide dismutase, 120 horseradish peroxidase, and 20 fl ...201222741539
heterologous enzyme linked immunosorbent assay for measurement of testosterone in serum.in steroid enzyme immunoassay (eia), homologous and heterologous combinations of enzyme conjugate with immunogen influences labeled steroid recognition by antibodies that affect sensitivity of the assay. to develop testosterone enzyme linked immunosorbent assay (elisa), antibodies were generated against testosterone-3-carboxymethyloxime-bovine serum albumin (t-3-cmo-bsa), testosterone-11-hemisuccinate-bovine serum albumin (t-11-hs-bsa), testosterone-17-hemisuccinate-bovine serum albumin (t-17-hs ...201222738649
sensitive csf elisas for the detection of mbl, masp-2 and functional mbl/masp-2.mannose binding lectin (mbl) mediated complement pathway is an important constituent of innate immune response in several infections including neuroinflammatory and neurodegenerative diseases. although there are enzyme-linked immunosorbent assays (elisas) for estimating mbl, mbl-associated serine protease-2 (masp-2) and functional mbl-masp-2 (fmbl) proteins for the plasma, serum and cell supernatants there are no established methods for their estimation in the cerebrospinal fluid (csf). we devel ...201222728252
electrochemical immunoassay for cd10 antigen using scanning electrochemical microscopy.in the present study, a scanning electrochemical microscopic (secm) method for imaging of antigen/antibody binding was proposed using cd10 antigen as the model. on the basis of anti-cd10 modified electrode, an electrochemical immunosensor for sensitive detection of cd10 antigen at low potential was developed by a multiple signal amplification strategy. gold nanoparticles (aunps) served as carriers to load more secondary antibodies (ab(2)) and horseradish peroxidase (hrp). the tip ultramicroelect ...201222727518
amplified electrochemical detection of a cancer biomarker by enhanced precipitation using horseradish peroxidase attached on carbon nanotubes.an electrochemical nanoimmunosensor based on multiwall carbon nanotubes (mwcnts)/gold nanoparticles (aunps) was developed for the amplified detection of prostate specific antigen (psa). the amplified detection was achieved by the enhanced precipitation of 4-chloro-1-naphthol (cn) using a higher number of horseradish peroxidase (hrp) molecules attached on mwcnts. the psa nanoimmunosensor was fabricated by immobilizing a monoclonal anti-psa antibody (anti-psa) on the aunp-attached thiolated mwcnt ...201222793977
protection of blood retinal barrier and systemic vasculature by insulin-like growth factor binding protein-3.previously, we showed that insulin growth factor (igf)-1 binding protein-3 (igfbp-3), independent of igf-1, reduces pathological angiogenesis in a mouse model of the oxygen-induced retinopathy (oir). the current study evaluates novel endothelium-dependent functions of igfbp-3 including blood retinal barrier (brb) integrity and vasorelaxation. to evaluate vascular barrier function, either plasmid expressing igfbp-3 under the regulation of an endothelial-specific promoter or a control plasmid was ...201222792172
nanogold-polyaniline-nanogold microspheres-functionalized molecular tags for sensitive electrochemical immunoassay of thyroid-stimulating hormone.methods based on nanomaterial labels have been developed for electrochemical immunosensors and immunoassays, but most involved low sensitivity. herein a novel class of molecular tags, nanogold-polyaniline-nanogold microspheres (gpgs), was first synthesized and functionalized with horseradish peroxidase-conjugated thyroid-stimulating hormone antibody (hrp-ab(2)) for sensitive electrochemical immunoassay of thyroid-stimulating hormone (tsh). x-ray diffraction, confocal raman spectroscopy, scanning ...201222790703
a highly efficient nano-cluster artificial peroxidase and its direct electrochemistry on a nano complex modified glassy carbon electrode.a nano-cluster with highly efficient peroxide activity was constructed based on nafion (nf) and cytochrome c (cyt c). uv-vis spectrometry and transmission electron microscopy (tem) methods were utilized for characterization of the nano-structured enzyme or artificial peroxidase (ap). the nano-cluster was composed of a chain-ball structure, with an average ball size of about 40 nm. the michaelis-menten (k(m)) and catalytic rate (k(cat)) constants of the ap were determined to be 2.5 ± 0.4 µm and 0 ...201222790375
morphology antibody chromosome technique for determining phenotype and genetic status of the same cell.the morphology antibody chromosome (mac) technique is a combination of methods that permits analysis of both phenotypic and genetic evaluation on a single interphase or mitotic cell as a basis for lineage analysis of neoplastic and normal cells. this unit describes mac with sequential phenotypic analysis using antibody and an alkaline phosphatase anti-alkaline phosphatase (apaap) complex and genotypic analysis using in situ hybridization with either enzymatic or fluorescence detection. alternate ...201222786612
investigation of glucosinolate profile and qualitative aspects in sprouts and roots of horseradish (armoracia rusticana) using lc-esi-hybrid linear ion trap with fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation.within the family of brassicaceae, an important source of glucosinolates (glss) is represented by horseradish ( armoracia rusticana p. gaertner, b. meyer & scherbius), cultivated for its roots, which are grated fresh or processed into a sauce and used as a condiment. the characteristic pungent flavor of the root depends on the abundance of the bioactive gls molecules. in crude plant extracts (sprouts and roots) of an accession of horseradish largely diffused in the basilicata region (southern it ...201222779710
screening of three commercial plant peroxidases for the removal of phenolic compounds in membrane bioreactors.a comparative study of three plant peroxidases, horseradish (hrp), soybean (sbp) and artichoke (akpc), was carried out to select the most appropriate one for 4-chlorophenol treatment in an ultrafiltration membrane reactor. soybean peroxidase showed the highest enzymatic activity, followed by hrp and akpc. the same tendency was observed in a discontinuous tank reactor, where sbp attained more than 90% of4-chlorophenol removal within the ph range tested. the optimum temperature was 30 degrees c, w ...201222720436
a mechanistic study of the formation of hydroxyl radicals induced by horseradish peroxidase with nadh.during the oxidation of nadh by horseradish peroxidase (hrp-fe(3+)), superoxide (o(-)(2)) is produced, and hrp-fe(3+) is converted to compound iii. superoxide dismutase inhibited both the generation of o(-)(2) and the formation of compound iii. in contrast, catalase inhibited only the generation of o(-)(2). under anaerobic conditions, the formation of compound iii did not occur in the presence of nadh, thus indicating that compound iii is produced via formation of a ternary complex consisting of ...201222718789
enzyme-linked small-molecule detection using split aptamer ligation.here we report an aptamer-based analogue of the widely used sandwich enzyme-linked immunosorbent assay (elisa). this assay utilizes the cocaine split aptamer, which is comprised of two dna strands that only assemble in the presence of the target small molecule. one split aptamer fragment is immobilized on a microplate, then a test sample is added containing the second split aptamer fragment. if cocaine is present in the test sample, it directs assembly of the split aptamer and promotes a chemica ...201222715870
identification of cell-surface molecular interactions under living conditions by using the enzyme-mediated activation of radical sources (emars) method.important biological events associated with plasma membranes, such as signal transduction, cell adhesion, and protein trafficking, are mediated through the membrane microdomains. we have developed a novel method termed enzyme-mediated activation of radical sources (emars) to identify coclustering molecules on the cell surface under living conditions, which features a radical formation from an aryl azide reagent by horseradish peroxidase (hrp). for identification of molecules labeled by the emars ...201223443365
effects of cytochrome p450 inhibitors on peroxidase activity.of several enzymes metabolizing xenobiotics, cytochrome p450 (cyp) and peroxidase enzymes seem to be most important. one of the major challenges in studies investigating metabolism of xenobiotics is to resolve which of these two groups of enzymes is predominant to metabolize individual xenobiotic compounds. utilization of selective inhibitors of cyp and peroxidase enzymes might be a useful tool to identify the contribution of these enzymes to metabolism of xenobiotics in samples, where both type ...201223353841
sequence analysis and homology modeling of peroxidase from medicago sativa.plant peroxidases are one of the most extensively studied group of enzymes which find applications in the environment, health, pharmaceutical, chemical and biotechnological processes. class iii secretary peroxidase from alfalfa (medicago sativa) has been characterized using bioinformatics approach physiochemical properties and topology of alfalfa peroxidase were compared with that of soybean and horseradish peroxidase, two most popular commercially available peroxidase preparations. lower value ...201223275690
protein cluster formation during enzymatic cross-linking of globular proteins.work on enzymatic cross-linking of globular food proteins has mainly focused on food functional effects such as improvements of gelation and enhanced stabilization of emulsions and foams, and on the detailed biochemical characterization of the cross-linking chemistry. what is still lacking is a physical characterization of cluster formation and gelation, as has been done for example, for cluster formation and gelation during heat-induced protein aggregation. here we present preliminary results a ...201223234160
silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase for imaging and therapeutic applications.mesoporous silica nanoparticles coencapsulating gadolinium oxide and horseradish peroxidase (hrp) have been synthesized in the aqueous core of sodium bis-(2-ethylhexyl) sulfosuccinate (aot)-hexane-water reverse micelle. the average diameter of these silica particles is around 25 nm and the particles are spherical and highly monodispersed as depicted using transmission electron microscopy. the entrapment efficiency of hrp was found to be as high as 95%. practically, the entrapped enzyme shows zer ...201223233799
new enzymatically polymerized copolymers from 4-tert-butylphenol and 4-ferrocenylphenol and their modification and inclusion complexes with β-cyclodextrin.the enzymatically catalyzed synthesis of a copolymer of 4-tert-butylphenol and 4-ferrocenylphenol by horse radish peroxidase (hrp) in the presence of h(2)o(2) in a 1,4-dioxane/water system is described. furthermore, polymer-analogous alkylation of the free hydroxy groups and subsequent click reaction with mono-6-azido-6-desoxy-β-cyclodextrin (n(3)-β-cd) was carried out. the formation of inter- and intramolecular inclusion complexes was investigated by dls measurement.201223243473
catalytic and inhibitory kinetic behavior of horseradish peroxidase on the electrode surface.enzymatic biosensors are often used to detect trace levels of some specific substance. an alternative methodology is applied for enzymatic assays, in which the electrocatalytic kinetic behavior of enzymes is monitored by measuring the faradaic current for a variety of substrate and inhibitor concentrations. here we examine a steady-state and pre-steady-state reduction of h(2)o(2) on the horseradish peroxidase electrode. the results indicate the substrate-concentration dependence of the steady-st ...201223202175
properties and analytical applications of the self-assembled complex {peroxidase-chitosan}.a novel promising approach to the improvement of analytical properties of horseradish peroxidase based on its inclusion into self-assembled structures of chitosan is discussed. it is shown that the reasonable choice of a polyelectrolyte, a detailed investigation of its interaction with the enzyme and the conditions of the {peroxidase-polyelectrolyte} complex formation allow for stabilizing the biocatalyst in aqueous and aqueous-organic media without a substantial loss in its activity and develop ...201223182581
integrated tyramide and polymerization-assisted signal amplification for a highly-sensitive immunoassay.a novel strategy for ultrasensitive detection of model protein based on the integration of tyramide signal amplification (tsa) and polymerization-assisted signal amplification was proposed. the surface-initiated atom transfer radical polymerization (si-atrp) of glycidyl methacrylate (gma) was triggered by the initiator-coupled protein immobilized on the electrode surface through sandwiched immunoreactions. growth of long chain polymeric materials provided numerous epoxy groups for subsequent cou ...201223181414
degradation of textile dyes using immobilized lignin peroxidase-like metalloporphines under mild experimental conditions. 201223256784
block copolymer-quantum dot micelles for multienzyme colocalization.to mimic the structure and functionality of multienzyme complexes, which are widely present in nature, pluronic-based micelles were designed to colocalize multiple enzymes. to stabilize the micelles as well as to enable characterization of single enzyme immobilization and multienzyme colocalization by förster resonance energy transfer (fret), quantum dots (qds) were incorporated into the micelles to form pluronic-qd micelles using a novel microreactor. model enzymes glucose oxidase (gox) and hor ...201223171402
[fundamental and applied aspects of the hematoencephalic barrier research].the results of fundamental and applied studies of blood-brain barrier had been conducted by authors during the last 10 years are summarized in the publication. the molecular anatomy of barrier microvessels, as well as promising markers of bbb and other proteins involved in barrier functions are discussed. via in vitro experiments with endothelial cells of cerebral microvessels we characterized the basic conditions required for adequate bbb modeling. the in vivo data of bbb permeability for macro ...201223166992
blood-brain barrier, epileptogenesis, and treatment strategies in cortical dysplasia.cortical dysplasia (cd) is one of the most important causes of intractable epilepsy. the precise mechanisms of epileptogenesis in cd are not known. using cd animal models, we attempted to understand the mechanisms and efficacy of various antiepileptic drugs. in two separate studies, we assessed (1) the effects of levetiracetam (lev) and vagus nerve stimulation (vns) on pentylenetetrazol (ptz)-kindled rats, and (2) the effects of lev and topiramate (tpm) on rats with cd and hyperthermia (ht). in ...201223134493
dynamic model of hydrogen peroxide diffusion kinetics into the pulp cavity.to measure the time course hydrogen peroxide penetration into the pulp cavity and evaluate short-term tooth color changes after bleaching.201223151689
strategy to enhance the wettability of bioacive paper-based sensors.this paper reports a potential method that can restore the wettability of bioactive paper-based sensors while maintaining their bioactivity. this study is driven by the need to increase the wettability of the antibody-loaded blood typing paper devices in order to increase the blood typing assaying speed using such paper devices. plasma treatment is used to improve the wettability of bioactive paper; the protective effect of bovine serum albumin (bsa) to biomolecules against plasma deactivation i ...201223151066
metabolism of diclofenac in plants--hydroxylation is followed by glucose conjugation.pharmaceuticals from human or veterinary medication form a new class of micropollutants that poses a serious threat to our aquatic environment and its organisms. the intensively used nonsteroidal anti-inflammatory drug diclofenac is found in the environment worldwide due to its poor elimination during waste water treatment processes. in order to test phytoremediation as a tool for the removal of this drug from waste water, the uptake of the compound into plant tissues and its metabolic pathway w ...201223137548
elcr: electrochemical detection of single dna base changes via ligase chain reaction.simple, inexpensive and wide-scaled analysis of single dna base changes ('point mutations' which include snp's, somatic mutations and epigenetic changes) is one of the holy grail's of point-of-care diagnostics. herein, we present elcr: a simple methodology that fuses ligase chain reaction (lcr) with electrochemical detection based on dna-mediated charge transport. lcr generates long and short "knife" motifs representing the presence or absence of single dna base changes, which are then detected ...201223133830
zonal distribution of perforant path cells in layer iii of the entorhinal area projecting to ca1 and subiculum in the rat.the distribution pattern of the cells that give rise to perforant path projections, including direct entorhino-ca1 and entorhino-subicular projections, was investigated in layer iii of the medial and lateral entorhinal areas in the rat using retrograde labeling with horseradish peroxidase conjugated to wheat germ agglutinin and cholera toxin b subunit. using two-dimensional unfolded maps of the entire hippocampal and parahippocampal fields, we found that cells projecting to a certain septotempor ...201223131426
stabilization of enzymatically polymerized phenolic chemicals in a model soil organic matter-free geomaterial.a variety of remediation methods, including contaminant transformation by peroxidase-mediated oxidative polymerization, have been proposed to manage soils and groundwater contaminated with chlorinated phenols. phenol stabilization has been successfully observed during cross polymerization between phenolic polymers and soil organic matter (som) for soils with som >3%. this study evaluates peroxidase-mediated transformation and removal of 2,4-dichlorophenol (dcp) from an aqueous phase in contact w ...201223128748
dioxetane-doped silica nanoparticles as ultrasensitive reagentless thermochemiluminescent labels for bioanalytics.thermochemiluminescence (tcl; the light emission originating by the thermally triggered decomposition of a molecule) was proposed in the late 1980s as a detection technique for immunoassays. however, after little pioneering work, this technique was abandoned because of the high temperatures required and the poor detectability in comparison to other labels. here we describe for the first time a thermochemiluminescent acridine-based 1,2-dioxetane with a remarkably low (i.e., below 100 °c) emission ...201223121217
activation of hypothalamic gono-like neurons in female rats during estrus.in mammals, gonadal function is controlled by the activity of hypothalamic gonadotropin-releasing hormone neurons, which control the secretion of adenohypophyseal and gonadal hormones. however, there are a number of unanswered questions in relation to gonadal function. it is currently unknown how erotogenic stimulation of the genitals influences the subpopulation of hypothalamic medial preoptic area neurons, antidromically identified as projecting to the median eminence at different periods of t ...201225337091
development of polyclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of alicyclobacillus strains in apple juice.a sort of specific polyclonal anti-alicyclobacillus antibody was generated by immunizing new zealand white rabbits, and a sensitive indirect enzyme-linked immunosorbent assay (elisa) was developed for alicyclobacillus detection in apple juice. a set of experimental parameters such as concentration of antigen, dilutions of the antibody and goat anti-rabbit igg-horseradish peroxidase conjugate, selection of the blocking reagent, incubation time, and temperature was optimized. the cross-reactivity ...201223106215
single turnover studies of oxidative halophenol dehalogenation by horseradish peroxidase reveal a mechanism involving two consecutive one electron steps: toward a functional halophenol bioremediation catalyst.horseradish peroxidase (hrp) catalyzes the oxidative para-dechlorination of the environmental pollutant/carcinogen 2,4,6-trichlorophenol (2,4,6-tcp). a possible mechanism for this reaction is a direct oxygen atom transfer from hrp compound i (hrp i) to trichlorophenol to generate 2,6-dichloro 1,4-benzoquinone, a two-electron transfer process. an alternative mechanism involves two consecutive one-electron transfer steps in which hrp i is reduced to compound ii (hrp ii) and then to the ferric enzy ...201223102773
enzyme mimics of au/ag nanoparticles for fluorescent detection of acetylcholine.we have developed a highly sensitive and selective fluorescent assay for the detection of acetylcholine (ach) based on enzyme mimics of au/ag nanoparticles (nps). these nps were prepared via a one-step solution phase reaction between 13 nm au nps and ag(+) ions in the presence of stabilizing agents such as adenosine triphosphate (atp) and polyethylene glycol (peg). our sensing strategy involves reacting ach with acetylcholinesterase (ache) to form choline that is in turn oxidized by choline oxid ...201223101755
catalytic activity and stability of glucose oxidase/horseradish peroxidase co-confined in macroporous silica foam.investigation of the catalytic activity and stability of enzymes in confined nano/microspace provides valuable contributions to the fundamental understanding of biological reactions taking place on a mesoscopic scale within confined spaces. in this paper, macroporous silica foam (msf) is used as a nanoreactor to co-confine glucose oxidase (god) and horseradish peroxidase (hrp). then, the enzymatic cascade reactions, which act in tandem inside nanoreactors, for oxidation of glucose and 3,3',5,5'- ...201223096254
total parenteral nutrition induces a shift in the firmicutes to bacteroidetes ratio in association with paneth cell activation in rats.the use of total parenteral nutrition (tpn) in the treatment of critically ill patients has been the subject of debate because it has been associated with disturbances in intestinal homeostasis. important factors in maintaining intestinal homeostasis are the intestinal microbiota and paneth cells, which exist in a mutually amendable relationship. we hypothesized that the disturbed intestinal homeostasis in tpn-fed individuals results from an interplay between a shift in microbiota composition an ...201223096015
living target of ce(iii) action on horseradish cells: proteins on/in cell membrane.positive and negative effects of rare earth elements (rees) in life have been reported in many papers, but the cellular mechanisms have not been answered, especially the action sites of rees on plasma membrane are unknown. proteins on/in the plasma membrane perform main functions of the plasma membrane. cerium (ce) is the richest rees in crust. thus, the interaction between ce(iii) and the proteins on/in the plasma membrane, the morphology of protoplast, and the contents of nutrient elements in ...201223054867
development of an enhanced chemiluminescence immunoassay (clia) for detecting urinary albumin.in the present study, a sensitive and competitive chemiluminescence immunoassay (clia) was developed in order to detect human serum albumin (hsa) in urine specimen. the method utilizes a home-made monoclonal anti-albumin antibody conjugated to horseradish peroxidase enzyme (mab-hrp). sensitivity, specificity and linearity of the assay were evaluated. according to the results, the proper concentration of hsa and mab-hrp conjugates was 800 ng/100 μl and 1:200 respectively. in optimal conditions, t ...201223053982
electrochemical immunosensing platform for dna methyltransferase activity analysis and inhibitor screening.in this paper, we developed a novel electrochemical method to quantify dna methyltransferase (mtase) and analyze dna mtase activity. after the double dna helix structure was assembled on the surface of gold nanoparticle modified glassy carbon electrode, it was first methylated by m. sssi mtase and then digested by restriction endonuclease hpaii, which could not recognize the methylated cpg site. successively, anti-5-methylcytosine antibody was specifically conjugated on the cpg methylation site ...201223030620
a gel-based visual immunoassay for non-instrumental detection of chloramphenicol in food samples.a gel-based non-instrumental immuno-affinity assay was developed for the rapid screening of chloramphenicol (cap) in food samples with the limit of detection (lod) of 1 μg l(-1). the immuno-affinity test column (iatc) consisted of a test layer containing anti-cap antibody coupled gel, and a control layer with anti-hrp antibody coupled gel. based on the direct competitive immuno-reaction and the horseradish peroxidase enzymatic reaction, the test results could be evaluated visually. basically, bl ...201223084061
efficacy and safety of a combination herbal medicinal product containing tropaeoli majoris herba and armoraciae rusticanae radix for the prophylactic treatment of patients with respiratory tract diseases: a randomised, prospective, double-blind, placebo-controlled phase iii trial.the aim of this ich-gcp study was to investigate the efficacy and safety of a prophylactic administration of a combination herbal medicinal product (chmp) in two dosages compared to placebo with respect to the incidence of new occurring infections of the respiratory tract (rti). clinical experience of prophylactic treatment of respiratory tract infections with the marketed chmp containing horseradish root (armoraciae rusticanae radix) and nasturtium (tropaeoli majoris herba) has existed for deca ...201223083066
aquaporin-4 expression and blood-spinal cord barrier permeability in canalicular syringomyelia.noncommunicating canalicular syringomyelia occurs in up to 65% of patients with chiari malformation type i. the pathogenesis of this type of syringomyelia is poorly understood and treatment is not always effective. although it is generally thought that syringomyelia is simply an accumulation of csf from the subarachnoid space, the pathogenesis is likely to be more complex and may involve cellular and molecular processes. aquaporin-4 (aqp4) has been implicated in numerous cns pathological conditi ...201223082850
taml activator-based amperometric analytical devices as alternatives to peroxidase biosensors.the ferric taml catalysts [fe{c(6)h(2)-1,2-( ncocme(2)nco)(2)cme(2)}(oh(2))](-) (1) with counterions na(+) (a) and pph(4)(+) (b) function similar to horseradish peroxidase in the mediated electron transfer relays, which constitute a basis for amperometric biosensors. the mediators are mono- and bis-cyclometalated ru and os compounds of the type of [m(c∼n)(x)(n∼n)(3-x)](m+) with x = 1 and 2 (n∼n = 2,2'-bipyridine, (-)c∼n = 2-phenylpyridinato). cyclic voltammograms of the ru and os compounds are n ...201223005918
composite cryogel with immobilized concanavalin a for affinity chromatography of glycoproteins.composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. the composites with immobilized concanavalin a (con a) were used for capturing glycoproteins. adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. the monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions ...201223002026
in situ svvyglr peptide conjugation into injectable gelatin-poly(ethylene glycol)-tyramine hydrogel via enzyme-mediated reaction for enhancement of endothelial cell activity and neo-vascularization.tissue engineering therapies require biocompatible and bioactive biomaterials that are capable of encouraging an angiogenic response for effective tissue regeneration. in this study, a svvyglr peptide, which functions as a potent angiogenic factor, was conjugated into injectable gelatin-poly(ethylene glycol)-tyramine (gpt) hydrogels in situ to enhance endothelial cell activities and neo-vascularization. svvyglrggy (sv-y) conjugated gpt (sv-gpt) hydrogels were formed in situ via enzyme-mediated r ...201222998168
role of the ribose-specific marker furfuryl-amine in the formation of aroma active 1-(furan-2-ylmethyl)-1h-pyrrole (or furfuryl-pyrrole) derivatives.furfuryl-pyrroles possess a diverse range of organoleptic properties described as roasted, chocolaty, green, horseradish-like, and mushroom-like and are detected in various foods such as coffee, chocolate, popcorn, and roasted chicken. although their origin in food was attributed to furfuryl-amine, the latter has not been detected so far in maillard model systems or in foods. in this study, furfuryl-amine was shown to be formed specifically from ribose through nitrogen atom transfer from the α-a ...201222994507
a thin layer-based amperometric enzyme immunoassay for the rapid and sensitive diagnosis of respiratory syncytial virus infections.a simple electrochemical sandwich immunoassay involving a polystyrene microarray slide coated with monoclonal capture antibodies and carbon screen-printed sensors (sps) was designed for the rapid diagnosis of respiratory syncytial virus (rsv). the detection of the antibody-antigen complex formation relied on the use of a horseradish peroxidase conjugate. its chronoamperometric measurement detection was performed by confining a droplet of h(2)o(2)/3,3',5,5'-tetramethylbenzidine enzyme substrate/m ...201223141321
the prognostic value of the immunohistochemical aspects of tumor suppressor genes p53, bcl-2, pten and nuclear proliferative antigen ki-67 in resected colorectal carcinoma.this study aim was to identify the relations between tumor suppressor genes (p53, bcl-2, pten), nuclear proliferative antigen ki-67 and epidemiologic, morphologic and histologic patient related factors, in colorectal cancer.201222990545
superoxide reaction with tyrosyl radicals generates para-hydroperoxy and para-hydroxy derivatives of tyrosine.tyrosine-derived hydroperoxides are formed in peptides and proteins exposed to enzymatic or cellular sources of superoxide and oxidizing species as a result of the nearly diffusion-limited reaction between tyrosyl radical and superoxide. however, the structure of these products, which informs their reactivity in biology, has not been unequivocally established. we report here the complete characterization of the products formed in the addition of superoxide, generated from xanthine oxidase, to se ...201222989205
development and applications of simultaneous immunochemical staining and serial detection of overlapping proteins in blotting procedures.immunoblotting techniques are widely used for specific protein identifications and characterizations. the specific bindings of antibodies to epitopes in a protein sequence permits determination of antigens and gives detailed information about protein compositions and expression levels in complex suspensions. however the techniques are mostly restricted to one specific antibody determination. overlaying proteins are detected using numerous repeated gel runs. for multiple but specific protein dete ...201222974836
endocytic structures and synaptic vesicle recycling at a central synapse in awake rats.the synaptic vesicle (sv) cycle has been studied extensively in cultured cells and slice preparations, but not much is known about the roles and relative contributions of endocytic pathways and mechanisms of sv recycling in vivo, under physiological patterns of activity. we employed horseradish peroxidase (hrp) as an in vivo marker of endocytosis at the calyx of held synapse in the awake rat. ex vivo serial section scanning electron microscopy and 3d reconstructions revealed two categories of la ...201222974146
development and validation of an enzyme-linked immunosorbent assay for the quantification of cytochrome 3a4 in human liver microsomes.little is known about the influence of hepatic pathologies on cytochrome p450 (cyp) mediated drug metabolism in children. the determination of the abundance of the different isoforms in pediatric microsomes may provide valuable information on the mechanisms of possible changes in activity. until now, western blotting was mostly used for abundance measurements, but this technique only provides semi-quantitative data. therefore, this study aimed to develop and validate an indirect elisa for the qu ...201222967564
qualitative and quantitative evaluation of simon™, a new ce-based automated western blot system as applied to vaccine development.many ce-based technologies such as imaged capillary ief, ce-sds, cze, and mekc are well established for analyzing proteins, viruses, or other biomolecules such as polysaccharides. for example, imaged capillary isoelectric focusing (charge-based protein separation) and ce-sds (size-based protein separation) are standard replacement methods in biopharmaceutical industries for tedious and labor intensive ief and sds-page methods, respectively. another important analytical tool for protein character ...201222965727
characterization and enzyme-conjugation of a specific anti-l1 nanobody.persistent infection of the human papillomaviruses (hpv) has been shown to result in cervical cancer and intraepithelial neoplasia. early detection and screening programs are essential strategies against cervical cancer. a nanobody is the smallest antigen-binding fragment known and is derived from a camelid heavy-chain antibody. this tiny protein shows high solubility and stability. it can be produced cost-effectively with high yield production. in this study, we enriched a nanobody library agai ...201222963491
reactivity of nonsteroidal anti-inflammatory drugs with peroxidase: a classification of nonsteroidal anti-inflammatory drugs.to improve understanding of the essential effect of nonsteroidal anti-inflammatory drugs (nsaids) on prostaglandin h synthase (pghs), the reactivity of nsaids with peroxidases and the tyrosyl radical derived from myoglobin was examined.201222943177
screening for oxidative stress elicited by engineered nanomaterials: evaluation of acellular dcfh assay.the dcfh assay is commonly used for measuring free radicals generated by engineered nanomaterials (enm), a well-established mechanism of enm toxicity. concerns exist over susceptibility of the dcfh assay to: assay conditions, adsorption of dcfh onto enm, fluorescence quenching and light scattering. these effects vary in magnitude depending on enm physiochemical properties and concentration. a rigorous evaluation of this method is still lacking. the objective was to evaluate performance of the dc ...201222942866
improved covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate-based copolymers.a macroporous copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate, poly(gma-co-egdma), with various surface characteristics and mean pore size diameters ranging from 44 to 200 nm was synthesized, modified with 1,2-diaminoethane, and tested as a carrier for immobilization of horseradish peroxidase (hrp) by two covalent methods, glutaraldehyde and periodate. the highest specific activity of around 35 u g(-1) dry weight of carrier was achieved on poly(gma-co-egdma) copolymers with ...201222941271
a filtration method for rapid preparation of conjugates for immunoassay.modification of protein and other biopolymers by labeling them with small or macromolecules has become a very powerful research tool in biochemistry, molecular biology, diagnostics, and therapeutics. however, current methodologies available for their preparations are not straightforward and take several hours of incubation time. in this paper, we describe a new filtration-assisted technique for covalent conjugation between the reactive functional groups of two different molecules (small or macro ...201222935228
use of crude extract of lentil plant (lens culinaris medikus) in peroxidase-based analyses: fast kinetic determination of hydrogen peroxide and sarcosine in urine.peroxidase-catalysed reactions are used in a wide variety of analytical applications, most of them based on the final quantification of hydrogen peroxide. clinical tests for glucose, cholesterol, creatine, creatinine or uric acid in blood or urine and enzyme-linked immunosorbent assays for pesticides, hepatitis or acquired immune deficiency syndrome are good examples of such applications. the most widely used and commercially available peroxidase for biotechnological processes and analytical app ...201222932812
endothelial cell senescence is associated with disrupted cell-cell junctions and increased monolayer permeability. 201222929066
dnazyme-based rolling-circle amplification dna machine for ultrasensitive analysis of microrna in drosophila larva.we present a highly sensitive colorimetric method for microrna (mirna) detection. this method is based on a rolling-circle amplification (rca) dna machine, which integrates rca, nicking enzyme signal amplification and dnazyme signal amplification. the dna machine is triggered by the hybridization of target mirna with a rational designed padlock dna template and activated by rca. the resulting rca product then autonomously replicates a multiple machinery cutter cycle and generates accumulated amo ...201222928468
physical features of ultrasound assisted enzymatic degradation of recalcitrant organic pollutants.this work has attempted to provide answer to the interaction of sonolysis and enzymatic treatment on degradation of recalcitrant dyes in a combined treatment. the model system comprises of two dyes, acid red and malachite green as model pollutants, along with horseradish peroxidase as a model enzyme and ultrasound of 20 khz frequency. a dual approach of coupling experimental results with simulations of cavitation bubble dynamics has been adopted. utilization of oxidation potential of horseradish ...201221742538
digoxin immune fab protects endothelial cells from ouabain-induced barrier injury.problem  endogenous digitalis-like factors (edlf) inhibit sodium pump na(+) /k(+) atpase activity, and maternal edlf levels are elevated in preeclampsia (pe). this study determined whether digoxin immune fab (dif) could protect endothelial cells (ecs) from edlf-induced endothelial barrier dysfunction. method of study  ecs were treated with escalating doses of ouabain (a known edlf) in the presence or absence of dif. ec barrier integrity was examined by junction protein ve-cadherin and occludin e ...201221749548
differentiating between intra- and extracellular chemiluminescence in diluted whole-blood samples.the differentiation between extra- and intracellular production of reactive oxygen species (ros) in whole blood was measured by luminol- and isoluminol-enhanced chemiluminescence (cl).201221834798
double positivity to bee and wasp venom: improved diagnostic procedure by recombinant allergen-based ige testing and basophil activation test including data about cross-reactive carbohydrate determinants.specific ige (sige) antibodies to both bee and wasp venom can be due to a sensitivity to both insect venoms or due to cross-reactive carbohydrate determinants (ccds).201222421265
value of component based diagnostics in ige-mediated hymenoptera sting reactions.stings by insects can precipitate many signs and symptoms of dermatological and ocular diseases. of particular importance is the anaphylaxis after hymenoptera stings. selection of the appropriate venom for immunotherapy requires a precise diagnosis, which is frequently difficult to confirm since the history presented by the patient is many times not conclusive and diagnostic tests are often positive for bee venom (bv) and vespula venom (vv). this double positivity is either caused by true double ...201221995714
in vitro inhibitory activity of essential oil vapors against ascosphaera apis.this work evaluates the in vitro inhibitory activity of 70 essential oils (eos) in the vapor phase for the control of chalkbrood disease caused by ascosphaera apis maassen ex claussen (olive et spiltoir). two wild strains isolated from infected honey bee colonies together with one standard collection strain were tested by the microatmosphere method. from 70 eos, 39 exhibited an antifungal effect against a. apis standard and wild strains. the greatest antifungal action was observed for eo vapors ...201222474973
effects of the green tea catechin (-)-epigallocatechin gallate on trypanosoma brucei.the current pharmacopeia to treat the lethal human and animal diseases caused by the protozoan parasite trypanosoma brucei remains limited. the parasite's ability to undergo antigenic variation represents a considerable barrier to vaccine development, making the identification of new drug targets extremely important. recent studies have demonstrated that fatty acid synthesis is important for growth and virulence of trypanosoma brucei brucei, suggesting this pathway may have therapeutic potential ...201224533284
a feasible enzyme-linked immunosorbent assay system using monoclonal and polyclonal antibodies against glucosyltransferase-b from streptococcus mutans.streptococcus mutans has been considered the principal etiological agent of dental caries in humans. s. mutans can secrete three kinds of glucosyltransferases (gtfs). one of these, gtf-b, which synthesizes water-insoluble glucans from sucrose, has been considered to be one of the most important factors of cariogenic dental plaque formation. therefore, determination of whether gtf-b is present in plaque and saliva samples may contribute to the evaluation of individual virulence potential (caries ...201222741581
development of an elisa for the quantification of the c-terminal decapeptide prothymosin α(100-109) in sera of mice infected with bacteria.apoptosis is characterized by a series of discrete biochemical events, among which is the truncation of the nuclear polypeptide prothymosin alpha (protα) by activated caspase-3. this early apoptotic event results in the generation of a carboxy-terminal fragment of protα, the immunoactive decapeptide protα(100-109). we hypothesized that the detection of increased levels of protα(100-109) in serum can be directly correlated with the induction of massive cell apoptosis, resulting from a severe bact ...201323831611
an ultrasensitive peroxydisulfate electrochemiluminescence immunosensor for streptococcus suis serotype 2 based on l-cysteine combined with mimicking bi-enzyme synergetic catalysis to in situ generate coreactant.a novel signal amplification strategy of mimicking bi-enzyme synergetic catalysis to generate coreactant in situ was designed to fabricate an ultrasensitive peroxydisulfate electrochemiluminescence (ecl) immunosensor for detection of streptococcus suis serotype 2 (ss2). it was the first time to detect ss2 by using ecl. through the interaction between l-cysteine (l-cys) and hollow ptpd bimetal alloy nanoparticles (hptpd) to form ((l-cys-hptpd)n) nanocomposites, the loading amount of l-cys and hpt ...201323277341
a novel immunosensor for detecting toxoplasma gondii-specific igm based on goldmag nanoparticles and graphene sheets.a novel electrochemical immunosensor for detecting toxoplasma gondii-specific igm (tg-igm) was constructed based on goldmag (au-fe(3)o(4)) nanoparticles and graphene sheets (gs). thionine (thi), as a mediator, was first electropolymerized on a nafion-gs (nf-gs) modified electrode. subsequently, gold nanoparticles (aunps) were attached onto the poly-thionine film through π-stacking interactions, and then were used to immobilize toxoplasma gondii antigen (tg-ag) for immunosensor fabrication. a san ...201323010058
development of an elisa to detect the humoral immune response to trichinella zimbabwensis in nile crocodiles (crocodylus niloticus).crocodiles are known reservoir hosts of trichinella papuae and trichinella zimbabwensis, two zoonotic parasites that also infect mammals. since commercial crocodile farming represents a key source of income in several countries, it is important to monitor this nematode infection in both farmed crocodiles and in breeding stocks which are frequently introduced from the wild. for this purpose, an indirect elisa was developed to detect the anti-trichinella immune response in crocodile sera. new zeal ...201323433644
[effect of trichinella spiralis infection on expression and distribution of colonic epithelial e-cadherin in mice and its mechanism].to study the effect of trichinella spiralis (t.spiralis) infection on the expression and distribution of colonic epithelial e-cadherin in mice and its mechanism.201323608805
metal-binding ability of vip1: a bzip protein in arabidopsis thaliana.vire2-interacting protein 1 (vip1) is an arabidopsis thaliana bzip transcription factor which regulates pathogen responses and rehydration responses. vip1 has transcriptional activation potential, dna-binding ability, and a nuclear-cytoplasmic shuttling property. these functions are possibly regulated by cofactors and/or post-translational modifications. during an investigation of the functions of vip1, we discovered that vip1 can react with an ni²⁺-activated derivative of horseradish peroxidase ...201324057918
study of the thermoresistance of the allergenic ara h1 protein from peanut (arachis hypogaea).the effect of heat treatment on denaturation of ara h1 protein, a major allergen from peanut, was studied using several techniques. previously, ara h1 protein was isolated from raw peanut using ammonium sulfate precipitation and chromatograpic techniques. antibodies against ara h1 protein were obtained in rabbits, conjugated with horseradish peroxidase, and used to develop a sandwich elisa. denaturation of ara h1 protein was estimated by the loss of reactivity with its specific antibodies by eli ...201323469881
exogenous auxin affects the oxidative burst in barley roots colonized by piriformospora indica.beside a cardinal role in coordination of many developmental processes in the plant, the phytohormone auxin has been recognized as a regulator of plant defense. the molecular mechanisms involved are still largely unknown. using a sensitive chemiluminescence assay, which measures the oxidation of luminol in the presence of h₂o₂ by horseradish peroxidase (hrp), we report here on the ability of exogenously added indole-3-acetic acid (iaa) to enhance the suppressive effect of the root endophyte piri ...201323333979
the brainstem localization of gastric preganglionic parasympathetic neurons in the agouti (dasyprocta leporina).this study was designed to determine qualitatively, the source of gastric vagal nerve fibres in the agouti. a total of 18 male and female adult agoutis were used for the present investigation. following anaesthesia, laparotomy was performed and the stomach exteriorized. multiple intramuscular injections of wheat germ agglutinin-horseradish peroxidase (wga-hrp) were then made into different areas of the stomach in the experimental animals. the control animals were divided into four groups of two ...201324756734
production and purification of the multifunctional enzyme horseradish peroxidase.the oxidoreductase horseradish peroxidase (hrp) is used in numerous industrial and medical applications. in this review, we briefly describe this well-studied enzyme and focus on its promising use in targeted cancer treatment. in combination with a plant hormone, hrp can be used in specific enzyme-prodrug therapies. despite this outstanding application, hrp has not found its way as a biopharmaceutical into targeted cancer therapy yet. the reasons therefore lie in the present low-yield production ...201324683473
[quantitative detection of serum procollagen iii n-terminal peptide chemiluminescence enzyme immunoassay].to establish chemiluminescence enzyme immunoassay (cleia) for quantitative detection of procollagen iii n-terminal peptide (p iii np) in serum.201324645331
[establishment of a quantitative detection method for golgi protein73].to establish enzyme-linked immunosorbent assay (elisa) for quantitative detection of golgi protein73 (gp73) in serum.201324645330
[microplate chemiluminescence enzyme immunoassay for the quantitative analysis of tissue inhibitor of metalloproteinases i].to establish microplate chemiluminescence enzyme immunoassay (cleia) for quantitative analysis of tissue inhibitor of metalloproteinases i (timp i) in human serum.201324579481
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