Publications

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development of an electrochemical immunoassay for detection of gatifloxacin in swine urine.to detect gatifloxacin (gat) residue in swine urine, an electrochemical immunoassay was established. an indirect competitive immunoassay was developed, in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay (elisa) plate and gat residue from the sample competes with the limited binding sites in added anti-gat antibody. horseradish peroxidase (hrp) conjugated to goat anti-rabbit igg was used as the enzymatic label. a carbon fiber working electrode was constructed and ...201222302425
glucose oxidase/horseradish peroxidase co-immobilized at a cnt-modified graphite electrode: towards potentially implantable biocathodes. 201222298433
enzyme-nanoparticle conjugates at oil-water interface for amplification of electrochemical immunosensing.in the current research, the preparation of biocatalytic capsule (ab(2)-catalytic capsule) was based on utilizing "oil" (organic compound) to entrap the nano-au/hrp/hafp bioconjugates, which were produced by horseradish peroxidase (hrp) and hrp labeled anti-afp (hafp) co-conjugated on gold nanoparticles (nano-au). for the first time, a signal amplification strategy was introduced by the prepared biocatalytic capsule as labels for electrochemical immunodetection of α-1-fetoprotein (afp). the tran ...201222296802
patterned biocatalytic films via one-step self-assembly.patterned porous films containing enzymes have been facilely prepared via a one-step breath figure process, which is based on the self-assembly of horseradish peroxidase nanoparticles that show good resistance to organic solvents. the patterned biohybrid films possess robust catalytic activity.201222293594
a gene-protein assay for human epidermal growth factor receptor 2 (her2): brightfield tricolor visualization of her2 protein, the her2 gene, and chromosome 17 centromere (cen17) in formalin-fixed, paraffin-embedded breast cancer tissue sections.the eligibility of breast cancer patients for human epidermal growth factor receptor 2 (her2)-directed therapies is determined by the her2 gene amplification and/or her2 protein overexpression status of the breast tumor as determined by in situ hybridization (ish) or immunohistochemistry (ihc), respectively. our objective was to combine the us food and drug administration (fda)-approved her2 & chromosome 17 centromere (cen17) brightfield ish (bish) and her2 ihc assays into a single automated her ...201222647525
bi-enzyme synergetic catalysis to in situ generate coreactant of peroxydisulfate solution for ultrasensitive electrochemiluminescence immunoassay.a novel electrochemiluminescence (ecl) immunosensor for ultrasensitive detection of α-1-fetoprotein (afp) was designed based on the in situ bi-enzymatic reaction to generate coreactant of peroxydisulfate for signal amplification. in this work, aunps were electrodeposited on the glassy carbon electrode (gce) surface, which promoted the electron transfer. then, l-cysteine and another layer of aunps were, respectively assembled onto the modified electrode surface, which formed the multilayer films ...201222621981
modulation of tight junction proteins in the perineurium to facilitate peripheral opioid analgesia.peripheral application of opioids reduces inflammatory pain but is less effective in noninflamed tissue of rats and human patients. hypertonic solutions can facilitate the antinociceptive activity of hydrophilic opioids in noninflamed tissue in vivo. however, the underlying mechanisms are not well understood. we hypothesized that the enhanced efficacy of opioids may be because of opening of the perineurial barrier formed by tight junction-proteins like claudin-1.201222534246
a novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells.a novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. in the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. the double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid dna, ovalbumin (pi 4.5), horseradish peroxidase (pi 7.2), and cytochrome-c (pi 10 ...201222580226
astrocytes acquire morphological and functional characteristics of ependymal cells following disruption of ependyma in hydrocephalus.hydrocephalic hyh mutant mice undergo a programmed loss of the neuroepithelium/ependyma followed by a reaction of periventricular astrocytes, which form a new cell layer covering the denuded ventricular surface. we present a comparative morphological and functional study of the newly formed layer of astrocytes and the multiciliated ependyma of hyh mice. transmission electron microscopy, immunocytochemistry for junction proteins (n-cadherin, connexin 43) and proteins involved in permeability (aqu ...201222576081
the effect of chemical modification with pyromellitic anhydride on structure, function, and thermal stability of horseradish peroxidase.the stability of enzymes remains a critical issue in biotechnology. compared with the strategies for obtaining stable enzymes, chemical modification is a simple and effective technique. in the present study, chemical modification of horseradish peroxidase (hrp) was carried out with pyromellitic anhydride. hrp has achieved a prominent position in the pharmaceutical, chemical, and biotechnological industries. in this study, the effect of chemical modification on thermal stability, structure, and f ...201222562551
poly(o-phenylenediamine)-carried nanogold particles as signal tags for sensitive electrochemical immunoassay of prolactin.a novel class of redox-active molecular tags, poly(o-phenylenediamine)-carried nanogold particles (gppds), was first synthesized and functionalized with horseradish peroxidase-anti-prolactin conjugates (hrp-anti-prl). thereafter, a specific sandwich-type electrochemical immunoassay was designed for determination of prolactin (prl) by using gppd-labeled hrp-anti-prl conjugates as molecular tags on anti-prl antibody-modified glassy carbon electrode. compared with pure gold nanoparticles and poly(o ...201222560276
quantum dot based rapid tests for zearalenone detection.three different kinds of immunosorbent assays with luminescence detection were developed for the determination of zearalenone (zen), a secondary toxic metabolite of fusarium fungi. cdse/zns core/shell quantum dots (qds) were used as a label in quantitative micro-well plate immunoassays (fluorescent-labeled immunosorbent assay, flisa) and in qualitative column test methods. as carriers for qd-based column tests, sepharose gel (for covalent binding of antibody) and polyethylene frits (for physical ...201222555674
understanding the formation of cus concave superstructures with peroxidase-like activity.copper sulfide (cus) concave polyhedral superstructures (cpss) have been successfully prepared in an ethanolic solution by a simple solvothermal reaction without the use of surfactants or templates. two typical well defined, high symmetry cus concave polyhedrons, forming a concave truncated cuboctahedron and icosahedron were prepared. the effect of the reaction time, temperature and different cu ion and sulfur sources on the formation of cus cpss were investigated and a possible formation mechan ...201222552534
enhanced stability of horseradish peroxidase encapsulated in acetalated dextran microparticles stored outside cold chain conditions.micro- and nanoparticles have been shown to improve the efficacy of safer protein-based (subunit) vaccines. here, we evaluate a method of improving the vaccine stability outside cold chain conditions by encapsulation of a model enzyme, horseradish peroxidase (hrp), in an acid-sensitive, tunable biodegradable polymer, acetalated dextran (ac-dex). vaccines that are stable outside the cold chain would be desirable for use in developing nations. ac-dex particles encapsulating hrp were prepared using ...201222548844
comparative study of surface plasmon resonance, electrochemical and electroassisted chemiluminescence methods based immunosensor for the determination of antibodies against human growth hormone.an indirect immunoassay format with human growth hormone (hgh) immobilized on the self-assembled monolayer (sam) modified surface plasmon resonance (spr) chip has been shown to detect specific anti-hgh antibodies using the combination of three different physical phenomena in the same channel of the spr analyzer. for the enhancement of analytical signal and sensitivity of the immunosensor horseradish peroxidase (hrp) labeled secondary antibodies, specifically interacting with the formed immune co ...201222541890
microchip electrophoresis coupled with on-line magnetic separation and chemiluminescence detection for multiplexed immunoassay.a facile and universal strategy for multiplexed immunoassay is proposed. the strategy is based on microchip electrophoresis (mce) coupled with on-line magnetic separation and chemiluminescence (cl) detection. the system consisted of a microchip, an electromagnet, and a photomultiplier. the realization of multiplexed immunoassay protocol involves sampling magnetic nanoparticles (mnps) labeled antibodies, n-(4-aminobutyl)-n-ethyl-isoluminol (abei) labeled antigens and free antigens in the precolum ...201222539323
boronic acid functionalized core-shell polymer nanoparticles prepared by distillation precipitation polymerization for glycopeptide enrichment.the boronic acid-functionalized core-shell polymer nanoparticles, poly(n,n-methylenebisacrylamide-co-methacrylic acid)@4-vinylphenylboronic acid (poly(mba-co-maa)@vpba), were successfully synthesized for enriching glycosylated peptides. such nanoparticles were composed of a hydrophilic polymer core prepared by distillation precipitation polymerization (dpp) and a boronic acid-functionalized shell designed for capturing glycopeptides. owing to the relatively large amount of residual vinyl groups ...201222707097
high expression of cd98 alters epithelial barrier functions to promote induction of airway allergy.epithelial barrier dysfunction is critical in the induction of allergy; the aetiology is to be further understood. a recent report indicates that cd98 plays a role in the intestinal epithelial barrier dysfunction.201222702504
colorimetric detection of urine glucose based znfe2o4 magnetic nanoparticles.in this paper, we discovered that znfe(2)o(4) magnetic nanoparticles (mnps) possess intrinsic peroxidase-like activity. znfe(2)o(4) mnps exhibit several advantages such as high catalytic efficiency, good stability, monodispersion, and rapid separation over other peroxidase nanomimetics and horseradish peroxidase (hrp). znfe(2)o(4) mnps were used as a colorimetric biosensor for the detection of urine glucose. this method is simple, inexpensive, highly sensitive, and selective for glucose detectio ...201222702236
dual amplification strategy of highly sensitive thrombin amperometric aptasensor based on chitosan-au nanocomposites.a highly sensitive and selective electrochemical aptasensor for thrombin was developed. by introducing chitosan-gold nanoparticles and horseradish peroxidase (cs-aunps-hrp) conjugates to the sensitive union, the thrombin detection signal was dual amplified. the capture probe was prepared by immobilizing an anti-thrombin aptamer on core-shell fe(3)o(4)-au magnetic nanoparticles (aumnps) and which was served as magnetic separation material as well. the detection probe was prepared from another ant ...201222701874
a system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture.two lineages of endoderm develop during mammalian embryogenesis, the primitive endoderm in the pre-implantation blastocyst and the definitive endoderm at gastrulation. this complexity of endoderm cell populations is mirrored during pluripotent cell differentiation in vitro and has hindered the identification and purification of the definitive endoderm for use as a substrate for further differentiation. the aggregation and differentiation of early primitive ectoderm-like (epl) cells, resulting in ...201222701686
a direct hippocampo-cerebellar projection in chicken.our previous studies suggested that a direct hippocampo-cerebellar projection might exist in the chicken. to confirm such a presumption of hippocampo-cerebellar interactions, horseradish peroxidase (hrp) was used as a retrograde tracer to be injected into the white matter of the folia vi-viii of the cerebellum in young and adult chickens. in another set of experiments, young chickens were subjected to electrolytic lesions of the hippocampal formation (hf), especially the ventromedial portion, an ...201222692931
[development of a specific and sensitive enzyme-linked immunosorbent assay for vindesine].this paper reports a specific and sensitive enzyme-linked immunosorbent assay (elisa) for pharmacokinetic studies of vindesine (vds). anti-vds antibody was obtained by immunizing rabbits with vds conjugated with bovine serum albumin using n-[β-(4-diazophenyl) ethyl] maleimide as a heterobifunctional coupling agent. an enzyme marker was similarly prepared by coupling vds with horseradish peroxidase using n-(4-diazophenyl) maleimide. the detection limit of vds by elisa was approximately 24 pg/ml w ...201222687732
daf-fluorescence without no: elicitor treated tobacco cells produce fluorescing daf-derivatives not related to daf-2 triazol.diaminofluorescein-dyes (dafs) are widely used for visualizing no· production in biological systems. here it was examined whether daf-fluorescence could be evoked by other means than nitrosation. tobacco (nicotiana tabacum) suspension cells treated with the fungal elicitor cryptogein released compound(s) which gave a fluorescence increase in the cell-free filtrate after addition of daf-2 or daf-fm or dar-4m. daf-reactive compounds were relatively stable and identified as reaction products of h(2 ...201222683597
carbon, hydrogen, and nitrogen isotope fractionation associated with oxidative transformation of substituted aromatic n-alkyl amines.we investigated the mechanisms and isotope effects associated with the n-dealkylation and n-atom oxidation of substituted n-methyl- and n,n-dimethylanilines to identify isotope fractionation trends for the assessment of oxidations of aromatic n-alkyl moieties by compound-specific isotope analysis (csia). in laboratory batch model systems, we determined the c, h, and n isotope enrichment factors for the oxidation by mno(2) and horseradish peroxidase (hrp), derived apparent (13)c-, (2)h-, and (15) ...201222681573
immunoextraction of zinc proteins from human plasma using chicken yolk antibodies immobilized onto paramagnetic particles and their electrophoretic analysis.zinc(ii) as the only transition metal lacking redox activity is an essential part of approximately 10% proteins as a cofactor of these proteins. considering the fact that there are numerous zinc(ii) containing proteins, proteomics and metallomics studies aimed on them require accurate methods for preparation of real biological samples prior to their subsequent analysis using 2de and ms. for this purpose, we suggested a new method based on chicken anti-zinc antibodies and magnetizable particles. ...201222674189
lectin histochemistry to detect altered glycosylation in cells and tissues.lectins are naturally occurring carbohydrate-binding molecules. a very wide range of purified lectins are commercially available which exhibit a diversity of carbohydrate-binding preferences. they can be used in the laboratory to detect carbohydrate structures on, or in, cells and tissues in much the same way that purified antibodies can be employed to detect cell- or tissue-bound antigens using immunocytochemistry. as lectins can distinguish subtle alterations in cellular glycosylation, they ar ...201222674124
basic immunocytochemistry for light microscopy.immunocytochemistry, the identification of cell- or tissue-bound antigens in situ, by means of a specific antibody-antigen reaction, tagged microscopically by a visible label, has a remarkably wide range of applications. the basic techniques are straightforward and can be adapted to explore the localisation of virtually any molecule of interest to the researcher in samples of normal and/or malignant cells. heterogeneity can be mapped and loss or gain of immunoreactivity with tumour progression c ...201222674123
enzyme activity assay for horseradish peroxidase encapsulated in peptide nanotubes.encapsulation of horseradish peroxidase (hrp) inside a peptide nanotube (pnt) was demonstrated and its activity was measured. enzyme assay verified that 0.16 μg of the enzymes were encapsulated in 1mg of pnts. the encapsulation was also verified with tem, uv-vis spectroscopy, and ftir. the activity of the encapsulated hrp was examined for thermal stability, long-term storage stability, and resistance to a denaturant. they showed good storage stability, retaining its activity up to 90%, while the ...201222664191
charge heterogeneity of monoclonal antibodies by multiplexed imaged capillary isoelectric focusing immunoassay with chemiluminescence detection.characterization of charge heterogeneity of recombinant monoclonal antibodies (mabs) requires high throughput analytical methods to support clone selection and formulation screens. we applied the nanopro technology to rapidly measure relative charge distribution of mabs in early stage process development. the nanopro is a multiplexed capillary-based isoelectric immunoassay with whole-column imaging detection. this assay offers specificity, speed and sensitivity advantages over conventional capil ...201222663182
immobilized horseradish peroxidase on discs of polyvinyl alcohol-glutaraldehyde coated with polyaniline.discs of network polyvinyl alcohol-glutaraldehyde (pvag) were synthesized and coated with polyaniline (pani) using glutaraldehyde as a chemical arm (pvag-panig-hrp disc). the best conditions for the immobilization were established as about 1.0 mg ml(-1) of protein, for 60 min and ph 5.5. the soluble enzyme lost all of its activity after incubation at 70°c for 15 min, whereas the pvag-panig-hrp disc retained about half of the initial activity for pyrogallol. the same pvag-panig-hrp disc was used ...201222619582
rapidly curable chitosan-peg hydrogels as tissue adhesives for hemostasis and wound healing.chitosan-poly(ethylene glycol)-tyramine (cpt) hydrogels were rapidly formed in situ using horseradish peroxidase and hydrogen peroxide to explore their performance as efficient tissue adhesives. a poly(ethylene glycol) modified with tyramine was grafted onto a chitosan backbone to enhance the solubility of the chitosan and to crosslink into three-dimensional networks. the elastic modulus of the hydrogels could be controlled by changing the crosslinking conditions, and the mechanical strength inf ...201222617740
production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process.we developed an alginate-based hydrogel fiber enabling to enclose endothelial cells, degradable on-demand by alginate lyase, and having a cell adhesive surface. the hydrogel fiber was obtained by extruding an aqueous solution of 4% (w/v) alginate derivative possessing phenolic hydroxyl moieties (alg-ph) and horseradish peroxidase (hrp) into a flow of aqueous solution containing 0.3 mm h(2)o(2) and gelatin derivative possessing ph moieties (gelatin-ph). in the process, cross-linking of alg-ph res ...201222613056
chromogen-based immunohistochemical method for elucidation of the coexpression of two antigens using antibodies from the same species.the authors established a chromogen-based, double immunolabeling method using antibodies from the same species without any unwanted cross-reactivity. in addition, time-consuming staining steps were shortened by using polymer-based secondary antibodies. taking advantage of the nature of the chromogen 3-amino-9-ethylcarbazole (aec), which is used as a horseradish peroxidase substrate for antibody detection, the aec-derived signals in the first color development were easily eliminated by alcohol tr ...201222610462
optimization of horseradish peroxidase-catalyzed enhanced chemiluminescence reaction by full factorial design.using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ecr) catalyzed by horseradish peroxidase (hrp-c) in the presence of 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) and 4-morpholinopyridine (morp) as enhancers was performed. the effect of concentrations of sptz, hydrogen peroxide, morp, luminol, and tris on a ratio of peroxidase-catalyzed cl to background was studied. the use of the full 2(5) factorial design instead of "one-varia ...201222608439
an integrated electrochemical device based on immunochromatographic test strip and enzyme labels for sensitive detection of disease-related biomarkers.a novel electrochemical biosensing device that integrates an immunochromatographic test strip and a screen-printed electrode (spe) connected to a portable electrochemical analyzer was presented for rapid, sensitive, and quantitative detection of disease-related biomarker in human blood samples. the principle of the sensor is based on sandwich immunoreactions between a biomarker and a pair of its antibodies on the test strip, followed by highly sensitive square-wave voltammetry (swv) detection. h ...201222608414
design and preparation of a nanoprobe for imaging inflammation sites.to image inflammation sites, we developed a novel nanoparticle, hydroxylamine-containing nanoparticle (hanp), which emits an intense electron spin resonance (esr)-signal triggered by enzymatic oxidation reaction and ph-sensitive self-disintegration. the nanoparticle was prepared from an amphiphilic block copolymer, poly(ethylene glycol)-b-poly[4-(2,2,6,6-tetramethylpiperidine-1-hydroxyl)aminomethylstyrene] (peg-b-pmnt-h), which spontaneously forms a core-shell type polymeric micelle (particle di ...201222589050
[enzymatic synthesis of electroconductive biocomposites based on dna and optically active polyaniline].electroconductive interpolymer polyaniline complexes are synthesized on the dna matrix, using the method of oxidative polymerization of aniline with two different biocatalyzers: horseradish root peroxidase and micropiroxidase-11 biomimetic. the spectral characteristics and morphology of the acquired biocomposites have been studied. the stereospecificity of the acquired samples of interpolymer complexes is shown, depending on the biocatalyzers used. the results acquired indicate the important rol ...201222586909
zymographic assay of oxidases using peroxidase or hemin entrapped in polyacrylamide gel.this chapter describes a zymographic assay of oxidases which is based on a coupled peroxidase or hemin reaction. the enzymatic activity of oxidases (i.e., diamine oxidase/dao, glucose oxidase, galactose oxidase) can be directly monitored on polyacrylamide gels containing horseradish peroxidase or hemin, in the presence of their specific substrates and ortho-phenylenediamine (opda), an oxidizable chromogen. in the presence of hydrogen peroxide, opda is oxidized to azo-aniline, which led to well-d ...201222585525
carbon nanospheres-promoted electrochemical immunoassay coupled with hollow platinum nanolabels for sensitivity enhancement.two nanostructures including carbon nanospheres-graphene hybrid nanosheets (cns-gns) and hollow platinum nanospheres (hptns) were first synthesized by using direct electrolytic reduction and wet chemistry methods, respectively. thereafter, a specific sandwich-type electrochemical immunoassay was designed for determination of carcinoembryonic antigen (cea) by using hptns-labeled horseradish peroxidase-anti-cea conjugates (hrp-anti-cea) as molecular tags and anti-cea-assembled cns-gps as sensing p ...201222487011
colorimetric detection of cholesterol with g-quadruplex-based dnazymes and abts2-.a novel colorimetric method for detection of cholesterol was developed with hemin-g-quadruplex dnazyme by transducing oxidation of cholesterol into the color change of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (abts(2-)). oligonucleotide 5'-gtgggtagggcgggttgg-3' (oligo-1) formed g-quadruplex structure in the presence of k(+), it acted as a horseradish peroxidase (hrp) mimicking dnazyme when binding hemin and catalyzed the oxidation of colorless abts(2-) to green abts ...201222483213
dnazyme-based turn-on chemiluminescence assays in homogenous media.in this work, novel biosensing systems were developed for dnazyme-based assays in homogenous aqueous media. the two halves of a horseradish peroxidase mimicking dnazyme were assembled onto different gold nanoparticle surfaces through hybridization with corresponding linking dna sequences. in the analyses, the target molecules were recognized by the linking dna. this recognition broke the hybridization and released the dnazyme halves from the nanoparticle surface into the solution. together, both ...201222465444
electrically contacted enzyme based on dual hairpin dna structure and its application for amplified detection of hg2+.in the present study, based on a dual hairpin dna structure, a novel system of electrically contacted enzyme and its signal amplification for ultrasensitive detection of hg(2+) was demonstrated. in the presence of hg(2+), with the interaction of thymine-hg(2+)-thymine (t-hg(2+)-t), dna sequence dully labeled with ferrocene (fc) at 5' end and horseradish peroxidase (hrp) at 3' end, hybridized to the capture probe and formed the dual hairpin structure on the electrode. fc unit acts as a relay that ...201222464917
delivery of large molecules via poly(butyl cyanoacrylate) nanoparticles into the injured rat brain.poly(n-butyl-2-cyanoacrylate) (pbca) nanoparticles have been successfully applied to deliver small-molecule drugs to the central nervous system (cns). however, it is unclear whether pbca nanoparticles can be used as the delivery system for large molecules to potentially treat traumatic brain injury (tbi). in this study, we tested the capacity of pbca nanoparticles in passing through the blood-brain barrier (bbb) and transporting large molecules into normal and injured brains in the rat. we first ...201222460562
functionalization of carbon buckypaper for the sensitive determination of hydrogen peroxide in human urine.here we report on a new approach for the electrochemical detection of hydrogen peroxide (h(2)o(2)) based on the co-immobilization of horseradish peroxidase and methylene blue on the functionalized carbon buckypaper supported by a titanium substrate. cyclic voltammetry was used to study and optimize the performance of the resulting electrochemical biosensor. the proposed biosensor exhibited high analytical performance towards the quantification of h(2)o(2) at the physiological ph 7.4. under optim ...201222459585
cross-linking of horseradish peroxidase adsorbed on polycationic films: utilization for direct dye degradation.horseradish peroxidase (hrp) was immobilized on the polyaniline (pani) grafted polyacrylonitrile (pan) films. the maximum hrp immobilization capacity of the pan-g-pani-3 film was 221 μg/cm(2). the hrp-immobilized pan-g-pani-3 film retained 79 % of the activity of the same quantity free enzyme. the hrp-immobilized pan-g-pani-3 film was operated for the decolorization of two different benzidine-based dyes in the presence of hydrogen peroxide. the maximum decolorization grade was obtained at ph 6.0 ...201222451080
γ-aminobutyric acid-, glycine-, and glutamate-immunopositive boutons on mesencephalic trigeminal neurons that innervate jaw-closing muscle spindles in the rat: ultrastructure and development.unlike other primary sensory neurons, the neurons in the mesencephalic trigeminal nucleus (vmes) receive most of their synaptic input onto their somata. detailed description of the synaptic boutons onto vmes neurons is crucial for understanding the synaptic input onto these neurons and their role in the motor control of masticatory muscles. for this, we investigated the distribution of γ-aminobutyric acid (gaba)-, glycine-, and glutamate-immunopositive (+) boutons on vmes neurons and their ultra ...201222430513
reactive intermediates-induced potential responses of a polymeric membrane electrode for ultrasensitive potentiometric biosensing.reactive intermediates rather than stable reactants or products have been found to induce large potential responses on an appropriately formulated polymeric membrane electrode, which provides a novel methodology for ultrasensitive potentiometric biosensing based on the horseradish peroxidase-h(2)o(2) oxidation reaction.201222430082
dual photonic-electrochemical lab on a chip for online simultaneous absorbance and amperometric measurements.a dual lab on a chip (dloc) approach that enables simultaneous optical and electrochemical detection working in a continuous flow regime is presented. both detection modes are integrated for the first time into a single detection volume and operate simultaneously with no evidence of cross-talk. the electrochemical cell was characterized amperometrically by measuring the current in ferrocyanide solutions at +0.4 v vs gold pseudoreference electrode, at a flow rate of 200 μl min(-1). the experiment ...201222429156
calcium carbonate-gold nanocluster hybrid spheres: synthesis and versatile application in immunoassays.fluorescent gold nanoclusters (auncs) were incorporated into porous calcium carbonate spheres through electrostatic interaction. the resulting caco(3)/auncs hybrid material exhibited interesting properties, such as porous structure, excellent biocompatibility, good water solubility, and degradability. these properties make the caco(3)/auncs hybrid material a promising template to assemble horseradish peroxidase/antibody conjugates (hrp-ab(2)). by using caco(3)/auncs/hrp-ab(2) bioconjugates as pr ...201222422592
graphene-based chemiluminescence resonance energy transfer for homogeneous immunoassay.we report on chemiluminescence resonance energy transfer (cret) between graphene nanosheets and chemiluminescent donors. in contrast to fluorescence resonance energy transfer, cret occurs via nonradiative dipole-dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. we designed a graphene-based cret platform for homogeneous immunoassay of c-reactive protein (crp), a key marker for human inflammation and cardiovascular diseas ...201222417160
bioconjugated and cross-linked bionanostructures for bifunctional immunohistochemical labeling.the present study describes the development and use of a new bioconjugate combining targeted quantum dot labeling with an immunoperoxidase method and explores whether these bioconjugates could specifically and effectively label cu/zn superoxide dismutase (sod1). the new bioconjugate is designed for the examination of samples both under fluorescent and bright-field microscopy at the same time. for this purpose chlorobis(2-2'-bipyridyl) methacryloyl tyrosine-ruthenium(ii) and bis (2-2'-bipyridyl) ...201222414278
interenzyme substrate diffusion for an enzyme cascade organized on spatially addressable dna nanostructures.spatially addressable dna nanostructures facilitate the self-assembly of heterogeneous elements with precisely controlled patterns. here we organized discrete glucose oxidase (gox)/horseradish peroxidase (hrp) enzyme pairs on specific dna origami tiles with controlled interenzyme spacing and position. the distance between enzymes was systematically varied from 10 to 65 nm, and the corresponding activities were evaluated. the study revealed two different distance-dependent kinetic processes assoc ...201222414276
characterization of multifunctional nanosystems based on the avidin-nucleic acid interaction as signal enhancers in immuno-detection.the avidin-nucleic-acids-nano-assembly (ananas) is a kind of soft poly avidin nanoparticle originating from the high affinity interaction between avidin and the nucleic acids. in this work we investigated the possibility of transforming ananas cores into stoichiometrically controlled multifunctional nanoparticles through a "one-pot" procedure, and we measured in a quantitative way their ability to work as reagents for enhanced immunodiagnostic detection. initially, we measured the ananas loading ...201222414051
bioactivity of horseradish peroxidase entrapped in silica nanospheres.interest in the fabrication of micro/nanoreactors for evaluation of the function of biomolecules in biological processes, enzymatic reaction kinetics occurring inside the nanospace is rapidly increasing. with a simple reverse-micelle microemulsion method, horseradish peroxidase (hrp), a model biomolecule, was herein skillfully confined in silica nanoshells (hrp@sio(2)) and its biocatalytical behaviors were investigated in detail. spectroscopic measurements showed that the entrapped hrp molecules ...201222410482
an electrochemically reduced graphene oxide-based electrochemical immunosensing platform for ultrasensitive antigen detection.we present an electrochemically reduced graphene oxide (ergo)-based electrochemical immunosensing platform for the ultrasensitive detection of an antigen by the sandwich enzyme-linked immunosorbent assay (elisa) protocol. graphene oxide (go) sheets were initially deposited on the amine-terminated benzenediazonium-modified indiun tin oxide (ito) surfaces through both electrostatic and π-π interactions between the modified surfaces and go. this deposition was followed by the electrochemical reduct ...201222409529
facile fabrication of gold nanoparticles-poly(vinyl alcohol) electrospun water-stable nanofibrous mats: efficient substrate materials for biosensors.electrospun nanofibrous mats are intensively studied as efficient scaffold materials applied in the fields of tissue engineering, catalysis, and biosensors due to their flexibility and porosity. in this paper, we report a facile route to fabricate gold nanoparticles-poly(vinyl alcohol) (au nps-pva) hybrid water stable nanofibrous mats with tunable densities of au nps and further demonstrate the potential application of as-prepared au nps-pva nanofibrous mats as efficient biosensor substrate mate ...201222409429
interaction between la(iii) and proteins on the plasma membrane of horseradish.lanthanum (la) is an important rare earth element in the ecological environment of plant. the proteins on the plasma membrane control the transport of molecules into and out of cell. it is very important to investigate the effect of la(iii) on the proteins on the plasma membrane in the plant cell. in the present work, the interaction between la(iii) and proteins on the plasma membrane of horseradish was investigated using optimization of the fluorescence microscopy and fluorescence spectroscopy. ...201222407212
quantification of domoic acid in shellfish samples by capillary electrophoresis-based enzyme immunoassay with electrochemical detection.a new method was developed to quantify domoic acid by capillary electrophoresis-based enzyme immunoassay (ce-eia) with electrochemical (ec) detection. the method was based on noncompetitive immunoreaction between free domoic acid antigen (ag) and excessive amount of horseradish peroxidase (hrp)-labeled antidomoic acid antibody tracer (ab*) in liquid phase. then the bound enzyme-labeled complex (ab*-ag) and unbound ab* were separated by ce, and the system of hrp catalyzing h(2)o(2)/o-aminophenol ...201222406515
core/shell nanofibers with embedded liposomes as a drug delivery system.the broader application of liposomes in regenerative medicine is hampered by their short half-life and inefficient retention at the site of application. these disadvantages could be significantly reduced by their combination with nanofibers. we produced 2 different nanofiber-liposome systems in the present study, that is, liposomes blended within nanofibers and core/shell nanofibers with embedded liposomes. herein, we demonstrate that blend electrospinning does not conserve intact liposomes. in ...201222401557
effects of operating parameters on the efficiency of liposomal encapsulation of enzymes.encapsulation of active proteins in the hydrophilic core of vesicular liposomes is important for developing a therapeutic protein carrier system. the efficiency of liposomal encapsulation of proteins is generally low. a better understanding of the fundamental mechanisms of encapsulation is needed to increase this efficiency. in this study we investigated the effects of operating parameters such as phospholipid concentration, buffer ph and ionic strength, protein size and surface charge, and lipo ...201222398367
in praise of h₂o₂, the versatile ros, and its vanadium complexes.hydrogen peroxide (h₂o₂) is generated in mitochondria in aerobic cells as a minor product of electron transport, is inhibited selectively by phenolic acids (in animals) or salicylhydroxamate (in plants) and is regulated by hormones and environmental conditions. failure to detect this activity is due to presence of h₂o₂-consuming reactions or inhibitors present in the reaction mixture. h₂o₂ has a role in metabolic regulation and signal transduction reactions. a number of enzymes and cellular acti ...201222394337
electrochemical detection of natural dna damage induced by in situ peroxidase-generated reactive nitrogen species in dna layer-by-layer films.the no(2)• radical is one kind of reactive nitrogen species and can be generated by horseradish peroxidase (hrp)-catalyzed oxidation of nitrite (no(2)(-)) in the presence of hydrogen peroxide (h(2)o(2)) and may cause dna damage in living system. in the present work, {pdda/dna}(4) layer-by-layer (lbl) films assembled with poly(diallyldimethylammonium chloride) (pdda) and natural double-stranded dna on electrodes were used to detect dna damage induced by no(2)• radicals generated from the hrp+h(2) ...201222387104
assessment of reactive oxygen species production in cultured equine skeletal myoblasts in response to conditions of anoxia followed by reoxygenation with or without exposure to peroxidases.to culture equine myoblasts from muscle microbiopsy specimens, examine myoblast production of reactive oxygen species (ros) in conditions of anoxia followed by reoxygenation, and assess the effects of horseradish peroxidase (hrp) and myeloperoxidase (mpo) on ros production.201222369537
hemigossypol, a constituent in developing glanded cottonseed (gossypium hirsutum).gossypol is a dimeric sesquiterpenoid first identified in cottonseed, but found in various tissues in the cotton plant including the seed. from its first discovery, it was assumed that hemigossypol was the biosynthetic precursor of gossypol. previous studies established that peroxidase (either from horseradish or from cottonseed) converts hemigossypol to gossypol. however, hemigossypol has never been identified in healthy cottonseed. in a temporal study using hplc and lc-ms, hemigossypol was ide ...201222369216
electrochemical immunosensor for carcinoembryonic antigen based on nanosilver-coated magnetic beads and gold-graphene nanolabels.a novel redox-active magnetic nanostructure was synthesized by using a wet chemical method for high-efficiency electrochemical immunoassay of carcinoembryonic antigen (cea, as a model analyte). the nanostructures based on the combination of a magnetic nanocore, a layer of electroactive poly(o-phenylenediamine) (ppd), and a silver metallic shell displayed good adsorption properties for the attachment of anti-cea antibody selective to cea. the magnetic nanostructure presented good redox behaviors ...201222365686
a hydrophilic and hydrophobic organic solvent mixture enhances enzyme stability in organic media.binary mixtures of hydrophilic and hydrophobic solvents were assessed for their ability to balance enzyme activity with the conservation of enzyme stability in organic media. acetone, dioxane and dodecane were chosen as model organic solvents, and subtilisin carlsberg and horseradish peroxidase (hrp) were chosen as model enzymes. residual enzyme activities were measured to monitor enzyme stability, and the fluorescence intensity of hrp was monitored to investigate structural changes due to the p ...201222361965
magnetosomes eliminate intracellular reactive oxygen species in magnetospirillum gryphiswaldense msr-1.magnetotactic bacteria synthesize magnetic particles called magnetosomes that cause them to orient to their external magnetic fields. however, the physiological significance and other possible functions of these magnetosomes have not been explored in detail. in this study, we have investigated the biological functions of magnetosomes with respect to their ability to scavenge reactive oxygen species (ros) in magnetospirillum gryphiswaldense msr-1. to assess the changes in ros levels under differe ...201222360568
biofunctionalized dendritic polyaniline nanofibers for sensitive electrochemical immunoassay of biomarkers.multi-armed dendritic polyaniline nanofibers (mpanfs) were first synthesized and functionalized with horseradish peroxidase (hrp) and carcinoembryonic antibody (anti-cea) for highly efficient electrochemical immunoassay of carcinoembryonic antigen (cea, as a model analyte here) in this work. transmission electron microscope (tem) and scanning electron microscope (sem) techniques were employed to characterize the synthesized mpanfs. by using anti-cea-conjugated core-shell gold-fe(3)o(4) nanocompo ...201222355804
enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells.an injectable hydrogel system, composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugates chemically cross-linked by an enzyme-mediated oxidation reaction, has been designed as a biodegradable scaffold for tissue engineering. in light of the role of substrate stiffness on cell differentiation, we herein report a newly improved gtn hydrogel system with a broader range of stiffness control that uses gtn-hpa-tyramine (gtn-hpa-tyr) conjugates to stimulate the osteogenic differentiation of ...201222343003
selective (1)o(2) quenchers, oligostilbenes, from vitis wilsonae: structural identification and biogenetic relationship.two previously unknown resveratrol trimers named wilsonols a-b, as well as a resveratrol tetramer named wilsonol c, were isolated from vitis wilsonae veitch, together with 12 known oligostilbenes. their chemical structures have been elucidated by detailed analyses of 1d and 2d nmr spectroscopic data, as well as chemical evidence obtained via either catalysis with hrp (horseradish peroxidase) and h(2)o(2) (hydrogen peroxide), acid, or uv irradiation. during the chemical processes, a biomimetic re ...201222342782
nano-structured arrays for multiplex analyses and lab-on-a-chip applications.nanospheres lithographic (nsl) method has been used to fabricate nano-structured arrays (nas) of hexagonally close-packed gold (au) using polystyrene beads [ps, diameter ∼300 nm] as mask. the developed na was incorporated with a customized and cheap microfluidics system to demonstrate its applicability as an alternative easy and efficient platform for multiplex analysis and lab-on-a-chip applications. the chip functionality was demonstrated with horseradish peroxidase (hrp) and anti-hrp antibody ...201222342717
binding of divalent metal ions to calcium-free peroxidase: thermodynamic and kinetic studies.thermodynamics of binding of divalent metal ions including ca(2+), mg(2+), ba(2+), and cd(2+) to ca-free horseradish peroxidase (hrp) enzyme was investigated using uv/vis spectrophotometry and molecular-mechanic (mm) calculations. according to the obtained binding and thermodynamic parameters, trend of the relative binding affinities of these divalent metal cations was found to be: ca(2+) > cd(2+) > mg(2+) > ba(2+). binding analysis based on scatchard and hill models showed positive cooperativit ...201222976971
development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles.the detection of estrogen receptors (ers) by immunohistochemistry (ihc) using 3,3'-diaminobenzidine (dab) is slightly weak as a prognostic marker, but it is essential to the application of endocrine therapy, such as antiestrogen tamoxifen-based therapy. ihc using dab is a poor quantitative method because horseradish peroxidase (hrp) activity depends on reaction time, temperature and substrate concentration. however, ihc using fluorescent material provides an effective method to quantitatively us ...201222959769
inhibitory effect of silicon nanowires on the polymerase chain reaction.the effect of nanomaterials on biological reactions has received much attention. we report herein that silicon nanowires (sinws) inhibit the polymerase chain reaction (pcr). the inhibitory effect was found to be concentration-dependent, with a minimum inhibitory concentration of about 0.4 mg ml(-1). dna polymerase, restriction endonucleases, lysozyme and horseradish peroxidase maintained their bioactivities after exposure to sinws. also the interaction of sinws with primers and dntp did not lead ...201222914382
[organization of the projections of the substructures of deep mesencephalic nucleus to the striatum in the dog brain].method of retrograde axonal transport of horseradish peroxidase was used to study the organization of the projections of the individual substructures of the deep mesencephalic nucleus complex to the functionally diverse regions of striatal structures of the dog brain (n=20). it was shown that the projection fibers of the neurons of the nucleus profundus mesencephali were directed to the ventro-lateral segment of the nucleus caudatus and the ventral segment of the putamen. the dorsal segment of t ...201222913133
bsa-templated mno2 nanoparticles as both peroxidase and oxidase mimics.inorganic nanomaterials that mimic enzymes are fascinating as they potentially have improved properties relative to native enzymes, such as greater resistance to extremes of ph and temperature and lower sensitivity to proteases. although many artificial enzymes have been investigated, searching for highly-efficient and stable catalysts is still of great interest. in this paper, we first demonstrated that bovine serum albumin (bsa)-stabilized mno(2) nanoparticles (nps) exhibited highly peroxidase ...201222900262
gold nanoparticles-based nanoconjugates for enhanced enzyme cascade and glucose sensing.we have coupled gold nanoparticles with horseradish peroxidase (hrp) to assemble catalytic nanoconjugates (hrp-aunps) for glucose detection. we found that a proper mixing ratio of hrp/aunps can significantly improve catalytic activity for the cascade reaction, an effect arising from increased spatial coupling between enzymes. such gold nanoparticle-based nanoconjugates are shown to be promising nanosensors for glucose.201222898737
a bioelectrochemical method for the quantitative description of the hofmeister effect of ionic liquids in aqueous solution.it is imperative to establish a simple, efficient, and practical method to investigate the hofmeister effect of ionic liquids (ils) on the behavior of proteins (enzymes). in this study, the effects of the cations and anions of different ils in aqueous media on the structural stability of horseradish peroxidase (hrp), a model oxidoreductase, were systematically investigated using electrochemical methods. it is found that without ils no direct electron transfer current signals of hrp appear at bar ...201222897433
enzymatic growth of quantum dots: applications to probe glucose oxidase and horseradish peroxidase and sense glucose.three innovative assays are developed for the detection of enzymatic activities of glucose oxidase (gox) and horseradish peroxidase (hrp) by the generation of cds quantum dots (qds) in situ using non-conventional enzymatic reactions. in the first assay, gox catalyzes the oxidation of 1-thio-β-d-glucose to give 1-thio-β-d-gluconic acid. the latter is spontaneously hydrolyzed to β-d-gluconic acid and h2 s, which in the presence of cadmium nitrate yields fluorescent cds nanoparticles. in the second ...201222887879
in vitro analysis of the monolignol coupling mechanism using dehydrogenative polymerization in the presence of peroxidases and controlled feeding ratios of coniferyl and sinapyl alcohol.in this study, dehydrogenative polymers (dhp) were synthesized in vitro through dehydrogenative polymerization using different ratios of coniferyl alcohol (ca) and sinapyl alcohol (sa) (10:0, 8:2, 6:4, 2:8, 0:10), in order to investigate the monolignol coupling mechanism in the presence of horseradish peroxidase (hrp), coprinus cinereus peroxidase (cip) or soybean peroxidase (sbp) with h(2)o(2), respectively. the turnover capacities of hrp, cip and sbp were also measured for coniferyl alcohol (c ...201222884779
dna methylation analysis triggered by bulge specific immuno-recognition.we report the sequence-selective discrimination of the cytosine methylation status in dna with anti methylcytosine antibody for the first time. this was realized by employing an affinity measurement involving the target methylcytosine in a bulge region and anti methylcytosine antibody, following hybridization with a bulge-inducing dna to ensure that only the target methylcytosine is located in the bulge. the affinity of the antibody for methylcytosine in the bulge was 79% of that in a single str ...201222880797
biotin igm antibodies in human blood: a previously unknown factor eliciting false results in biotinylation-based immunoassays.biotin is an essential vitamin that binds streptavidin or avidin with high affinity and specificity. as biotin is a small molecule that can be linked to proteins without affecting their biological activity, biotinylation is applied widely in biochemical assays. in our laboratory, igm enzyme immuno assays (eias) of µ-capture format have been set up against many viruses, using as antigen biotinylated virus like particles (vlps) detected by horseradish peroxidase-conjugated streptavidin. we recentl ...201222879954
improved performance of antigen-hrp conjugate-based immunoassays after the addition of anti-hrp antibody and application of a liposomal chemiluminescence marker.to overcome the sensitivity limit for small molecules (haptens) in immunoassays based on antigen-horseradish peroxidase (hrp) conjugates as labels, a novel approach was established that afforded very low detection limits. biotinylated anti-hrp antibody was utilized in order to attach, via a streptavidin bridge, liposomaly entrapped hrp. fentanyl, used as a model antigen, could be determined via the generation of a high-intensity and relatively stable chemiluminescence (cl) signal of a hrp-cataly ...201222878629
dna-directed immobilization of horseradish peroxidase onto porous sio2 optical transducers.multifunctional porous si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. first, an oxidized psi optical nanostructure, a fabry-pérot thin film, is synthesized and is used as the optical transducer element. immobilization of the enzyme onto the nanostructure is performed through dna-directed immobilization. preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. the c ...201222873686
ultrasensitive electrochemical immunosensor for ca 15-3 using thionine-nanoporous gold-graphene as a platform and horseradish peroxidase-encapsulated liposomes as signal amplification.this paper describes a novel electrochemical immunosensor using a nanoporous gold (npg)/graphene (gn) hybrid platform combined with horseradish peroxidase (hrp)-encapsulated liposomes as labels for the sensitive detection of cancer antigen 15-3 (ca 15-3). the electrochemical detection was based on the released hrp from hrp-encapsulated liposomes toward the reduction of h(2)o(2) with the help of the thionine (th) electron mediator. in the presence of ca 15-3, hrp@liposomes and th-npg-gn formed a ...201222866329
controlled immobilisation of active enzymes on the cowpea mosaic virus capsid.immobilisation of horseradish peroxidase (hrp) and glucose oxidase (gox) via covalent attachment of modified enzyme carbohydrate to the exterior of the cowpea mosaic virus (cpmv) capsid gave high retention of enzymatic activity. the number of enzymes bound per virus was determined to be about eleven for hrp and 2-3 for gox. this illustrates that relatively large biomacromolecules can be readily coupled to the virus surface using simple conjugation strategies. virus-biomacromolecule hybrids have ...201222865109
[establishment of ba-elisa method for detecting cea in human sera].to establish a sensitive biotin-avidin enzyme linked immunosorbent assay (ba-elisa) method for detecting carcinoembryonic antigen (cea) in serum.201222863598
a neurobehavioral analysis of the prevention of visual impairment in the dba/2j mouse model of glaucoma.timoptic-xe treatment was used to examine the relationship between age-related changes in intraocular pressure (iop), retinal cell loss, visual ability, and neuronal labeling in the superior colliculus in the dba/2j mouse model of pigmentary glaucoma.201222859742
whole-mount fluorescence in situ hybridization and antibody staining of drosophila embryos.whole-mount rna in situ hybridizations with digoxigenin-conjugated probes and alkaline phosphatase biochemistry have been used widely for many years to map expression pattern domains in the drosophila embryo. to capitalize on the number of molecular markers in the central nervous system (cns) and to enable expression analysis at the single-cell level, fluorescence in situ hybridization procedures are becoming standard. this protocol describes methods for the simultaneous detection of rna and pro ...201222854564
inhibition of heme peroxidases by melamine.in 2008 melamine-contaminated infant formula and dairy products in china led to over 50,000 hospitalizations of children due to renal injuries. in north america during 2007 and in asia during 2004, melamine-contaminated pet food products resulted in numerous pet deaths due to renal failure. animal studies have confirmed the potent renal toxicity of melamine combined with cyanuric acid. we showed previously that the solubility of melamine cyanurate is low at physiologic ph and ionic strength, pro ...201222852071
low concentration of silver nanoparticles not only enhances the activity of horseradish peroxidase but alter the structure also.chemical synthesis of ag-nps was carried out using reduction method. the reduction mechanistic approach of silver ions was found to be a basic clue for the formation of the ag-nps. the nanoparticles were characterized by uv-vis, ft-ir and tem analysis. we had designed some experiments in support of our hypothesis, "low concentrations of novel nanoparticles (silver and gold) increases the activity of plant peroxidases and alter their structure also", we had used ag-nps and hrp as models. the immo ...201222848490
redox-sensitive sulfenic acid modification regulates surface expression of the cardiovascular voltage-gated potassium channel kv1.5.kv1.5 (kcna5) is expressed in the heart, where it underlies the i(kur) current that controls atrial repolarization, and in the pulmonary vasculature, where it regulates vessel contractility in response to changes in oxygen tension. atrial fibrillation and hypoxic pulmonary hypertension are characterized by downregulation of kv1.5 protein expression, as well as with oxidative stress. formation of sulfenic acid on cysteine residues of proteins is an important, dynamic mechanism for protein regulat ...201222843785
biotin determination in food supplements by an electrochemical magneto biosensor.an electrochemical magneto biosensor for the rapid determination of biotin in food samples is reported. the affinity reaction was performed on streptavidin-modified magnetic microbeads as a solid support in a direct competitive format. the biotinylated horseradish peroxidase enzyme (biotin-hrp) competes with free biotin in the sample for the binding sites of streptavidin on the magnetic microbeads. the modified magnetic beads were then easily captured by a magneto graphite-epoxy composite electr ...201222841112
effect of organic solvents on peroxidases from rice and horseradish: prospects for enzyme based applications.a feasibility test for rice peroxidase (rp) enzyme as a substitute for horseradish peroxidase (hrp) was carried out. the activity of hrp was maximum at 30 °c with ph 6.0-7.0. the purified rice peroxidase showed optimum activity at 30 °c with ph 7-8 and was thermostable till 68 °c, which is higher than the temperature reported for hrp. rp obeyed michaelis-menten kinetics. with increasing substrate concentrations, rp and hrp had v(max) as 8.23 μm min(-1) and 4.21 μm min(-1) and k(m) as 5.585 and 3 ...201222841068
sensitive chemiluminescent immunoassay of triclopyr by digital image analysis.an image based detection of chemiluminescence enzyme-linked immunosorbent assay (cl-elisa) for the quantification of triclopyr has been developed. the immunoassay was an indirect competitive immunoassay with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (hrp). chemiluminescence was produced by the luminol/h(2)o(2)/hrp reaction, detected by a monochrome video ccd camera and digitized with an imagraph ic-pci frame grabber using a custom program developed in c(++) (microsof ...201222841045
synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides.in this study we describe a method for highly specific enrichment of glycopeptides with boronic acid-functionalized chitosan polymeric nanospheres and matrix assisted laser desorption-ionization mass spectrometry (maldi-ms). this is the first time chitosan has been used to create nanosphere support material for selective enrichment of glycopeptides by modification with glycidyl methacrylate (gma) and derivatization with 3-aminophenylboronic acid (apb). due to their multifunctional chemical moiet ...201222840004
a perioculomotor nitridergic population in the macaque and cat.we determined the distribution of cells containing synthetic enzymes for the unconventional neurotransmitter, nitric oxide, with respect to the known populations within the oculomotor complex.201222836763
[the influence of strabismus and monocular deprivation on the size of callosal cells in cortical areas 17 and 18 of the cat].to reveal the changes in visual cortex structure following impaired early binocular experience, the size (somatic area) of callosal cells in areas 17, 18 ofmonocularly deprived and convergent strabismic cats was measured. horseradish peroxidase was injected into the single ocular dominance columns of areas 17, 18 and the transition zone 17/18. in both groups of impaired cats the mean size of callosal cells in area 17 was increased in comparison to intact cats. in area 18, the similar difference ...201222834337
development of an automatic multi-channel ink-jet ejection chemiluminescence system and its application to the determination of horseradish peroxidase.in this work, an automatic multi-channel ink-jet for chemiluminescence (cl) analysis was developed. the four-channel ink-jet device was controlled by a home-made circuit. differing from the classic flow injection cl, the whole procedure for cl analysis was automatically completed on a hydrophobic glass side. cl reaction of luminal and hydrogen peroxide for the determination of horseradish peroxidase (hrp) was selected as an application to automatic cl analysis platform. all solutions delivered b ...201222819052
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