Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| electrochemical immunosensors for antibodies to peanut allergen ara h2 using gold nanoparticle-peptide films. | life-threatening allergies to peanuts and tree nuts can be revealed by detecting antibodies (iges) to their allergens in patient serum. herein, we compare several immunosensor-like methodologies for sensitive detection of antibodies to a peptide sequence from the major peanut allergen, arachis hypogaea 2 (ara h2). the sensors feature a synthetic peptide layer of the major ige-binding epitope from ara h2 attached to a dense gold nanoparticle (aunp) film on a pyrolytic graphite (pg) electrode. the ... | 2010 | 20540504 |
| lensfree holographic imaging of antibody microarrays for high-throughput detection of leukocyte numbers and function. | characterization of leukocytes is an integral part of blood analysis and blood-based diagnostics. in the present paper, we combine lensless holographic imaging with antibody microarrays for rapid and multiparametric analysis of leukocytes from human blood. monoclonal antibodies (abs) specific for leukocyte surface antigens (cd4 and cd8) and cytokines (tnf-alpha, ifn-gamma, il-2) were printed in an array so as to juxtapose cell capture and cytokine detection antibody (ab) spots. integration of ab ... | 2010 | 20359168 |
| carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation. | we have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. however, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotu ... | 2010 | 20364135 |
| oral bifidobacterium modulates intestinal immune inflammation in mice with food allergy. | it is proposed that probiotics have a therapeutic effect on the treatment of immune disorders. however, the underlying mechanisms require clarification. the present study aimed to evaluate the effect of gavage-feeding bifidobacteria on suppression of t helper 2 (th2) pattern inflammation in the intestines of mice with food allergy. | 2010 | 20546446 |
| accessory olfactory bulb function is modulated by input from the main olfactory epithelium. | although it is now established that sensory neurons in both the main olfactory epithelium and the vomeronasal organ may be activated by both general and pheromonal odorants, it remains unclear what initiates sampling by the vomeronasal organ. anterograde transport of wheat germ agglutinin-horseradish peroxidase was used to determine that adequate intranasal syringing with zinc sulfate interrupted all inputs to the main olfactory bulb but left intact those to the accessory olfactory bulb. adult m ... | 2010 | 20377623 |
| experimental study on acupuncture activating the gonadotropin-releasing hormone neurons in hypothalamus. | to probe into the most effective site, extra-ordinary point, acupoint and channel for regulating reproductive endocrine function by means of the study on acupuncture activating the gonadotropin-releasing hormone neurons (gnrh) in hypothalamus. | 2010 | 20397460 |
| meal parameters and vagal gastrointestinal afferents in mice that experienced early postnatal overnutrition. | early postnatal overnutrition results in a predisposition to develop obesity due in part to hypothalamic and sympathetic dysfunction. potential involvement of another major regulatory system component--the vagus nerve--has not been examined. moreover, feeding disturbances have rarely been investigated prior to development of obesity when confounds due to obesity are minimized. to examine these issues, litters were culled on the day of birth to create small litters (sl; overnutrition), or normal ... | 2010 | 20403369 |
| exploring peptide space for enzyme modulators. | a method is presented for screening high-density arrays to discover peptides that bind and modulate enzyme activity. a polyvinyl alcohol solution was applied to array surfaces to limit the diffusion of product molecules released from enzymatic reactions, allowing the simultaneous measurement of enzyme activity and binding at each peptide spot. for proof of concept, it was possible to identify peptides that bound to horseradish peroxidase, alkaline phosphatase, and beta-galactosidase and substant ... | 2010 | 20408521 |
| radio frequency radiation causes no nonthermal damage in enzymes and living cells. | the ability of radio frequency radiation (rfr) to exert irreversible nonthermal (i.e., not caused by accompanying heat) effects on biologics has been widely debated due to a relative paucity of comprehensive critical details in published reports dealing with this issue. in this study, we used rigorous control over experimental conditions to determine whether continuous rfr nonthermally affects commercially important enzymes and live bacterial and human cells using three most commonly used freque ... | 2010 | 20572294 |
| expression of astrocytic type 2 angiotensin receptor in central nervous system inflammation correlates with blood-brain barrier breakdown. | the blood-brain barrier (bbb), a complex of endothelial and glial barriers, controls passage of cells and solutes between the blood and central nervous system (cns). blood-brain barrier breakdown refers to entry of cells and/or solutes. we were interested whether the renin-angiotensin system is involved during bbb breakdown. we studied the type 2 angiotensin receptor at(2) because of its suggested neuroprotective role. two models of brain inflammation were used to distinguish solute versus cellu ... | 2010 | 20414743 |
| cholecystokinin/cholecystokinin-1 receptor-mediated peripheral activation of the afferent vagus by enteral nutrients attenuates inflammation in rats. | the current study investigates activation of the nutritional anti-inflammatory pathway by lipid-rich nutrition. | 2010 | 20585240 |
| determination of plasma and serum l-lysine using l-lysine epsilon-oxidase from marinomonas mediterranea nbrc 103028(t). | this article describes a successful application of l-lysine epsilon-oxidase (ec 1.4.3.20) for l-lysine determination. l-lysine epsilon-oxidase was isolated from culture supernatant of marinomonas mediterranea nbrc 103028(t) and was used for l-lysine determination. comparison of the characteristics of l-lysine epsilon-oxidase with l-lysine alpha-oxidase, a commercial enzyme used for l-lysine determination, suggests that the use of l-lysine epsilon-oxidase would be more valuable for the determinat ... | 2010 | 20599635 |
| simultaneous determination of biomarkers for alzheimer's disease using sol-gel-derived optical array biosensor. | the sol-gel-derived optical array-based biosensor for the simultaneous analysis of multiple analytes as potential markers for alzheimer's disease was fabricated. beta-amyloid, acetylcholine and glutamate were selected as the biosensing probes. the fluorescent dye, carboxy snarf-1-dextran, was co-immobilized with glutamate dehydrogenase and acetylcholinesterase for sensing glutamate and acetylcholine, respectively, while amplex red and fitc-dextran were immobilized with horseradish peroxidase for ... | 2010 | 20444591 |
| metabolic stability of superoxide and hydroxyl radical adducts of a cyclic nitrone toward rat liver microsomes and cytosol: a stopped-flow esr spectroscopy study. | the metabolic stability of the spin adducts derived from the reaction of superoxide and hydroxyl radicals with 5-tert-butoxycarbonyl-5-methyl-1-pyrroline n-oxide (bocmpo) in the presence of rat liver microsomes (rlm) and rat liver cytosol (rlc) was studied by using a stopped-flow device coupled to an electron spin resonance (esr) spectrometer. the kinetics of the disappearance of the bocmpo-oh and bocmpo-ooh radicals could be followed by esr spectroscopy with treatment of the esr data by an appr ... | 2010 | 20452418 |
| antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (raphanus sativus var. niger, brassicaceae). | the antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. the extract appeared to form a complex with fe(3+) or cu(2+). it displayed a concentration-dependant reducing power (1.16od(700 nm) at a concentration of 4mm) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with ic(50)=1.74 +/- 0.03 mm). it could promote the cle ... | 2010 | 20600520 |
| immunoassay for quantifying squamous cell carcinoma antigen in serum. | although the benefits of quantifying serum squamous cell carcinoma antigen (scca) have been reported, scca reagents were no longer available in the us by the late 1990s. because scca quantification still has demonstrated clinical utility, we developed and validated a microtiter plate-based elisa for measuring scca in serum. | 2010 | 20601447 |
| delivering horseradish peroxidase as a respirable powder to the isolated, perfused, and ventilated lung of the rat: the pulmonary disposition of an inhaled model biopharmaceutical. | our aim was to investigate the potential of the dustgun aerosol technology integrated with the isolated, perfused, and ventilated lung of the rat (ipl) to study the pulmonary disposition of an inhaled model biopharmaceutical, the 40-kda protein horseradish peroxidase (hrp). | 2010 | 20455769 |
| brain distribution and toxicological evaluation of a systemically delivered engineered nanoscale ceria. | engineered nanoscale ceria is used as a diesel fuel catalyst. little is known about its mammalian central nervous system effects. the objective of this paper is to characterize the biodistribution of a 5-nm citrate-stabilized ceria dispersion from blood into brain and its pro- or antioxidant effects. an approximately 4% aqueous ceria dispersion was iv infused into rats (0, 100, and up to 250 mg/kg), which were terminated after 1 or 20 h. ceria concentration, localization, and chemical speciation ... | 2010 | 20457660 |
| spinal motor neuronal degeneration after knee joint immobilization in the guinea pig. | this study used a unilateral knee joint immobilization model in adult guinea pigs to test the hypothesis that retrograde degeneration of motor neurons in the spinal cord is the result of attenuation of knee joint activities. | 2010 | 20605551 |
| recombinant peroxidase production in species of lepidoptera frequently found in argentina. | horseradish peroxidase isozyme c (hrpc) is an important commercial biocatalyst. in this study, a screening of different lepidopteran species frequently found in argentina to produce this protein was carried out. two recombinant viruses were constructed: acmnpv hrpc polyhedrin-minus (occ-), an intrahemocelical infective virus; and acmnpv hrpc polyhedrin-plus (occ+), to achieve an oral infective baculovirus. each lepidopteran species was infected either with acmnpv hrpc occ- or acmnpv hrpc occ+ an ... | 2010 | 20615485 |
| disposable amperometric magnetoimmunosensors for the specific detection of streptococcus pneumoniae. | disposable amperometric magnetoimmunosensors, based on the use of functionalized magnetic beads and gold screen-printed electrodes, have been developed for the selective detection and quantification of streptococcus pneumoniae. a specific antibody prepared against a serotype 37 s. pneumoniae strain, selected by flow cytometry among seven anticapsular or antisomatic antibodies, was linked to protein a-modified magnetic beads and incubated with bacteria. the same antibody, conjugated with horserad ... | 2010 | 20615685 |
| histochemical evaluation of human prostatic tissues with cratylia mollis seed lectin. | lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation. cratylia mollis seed lectins (cramoll 1,4 and cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (np), hyperplasia (bph), and prostate carcinoma (pca). the staining pattern of con-a and cramoll 1,4 in bph was more intense tha ... | 2010 | 20617135 |
| [segmental and regional innervation of acupoint "taixi" (ki 3) area in the rat: a horseradish peroxidase method study]. | to study the peripheral and central neural involvements of acupoint "taixi" (ki 3) by using horseradish peroxidase (hrp). | 2010 | 20458903 |
| coupled enzyme reactions in multicompartment microparticles. | spherical biopolymer particles have been fabricated, applying coprecipitation with calcium carbonate, followed by cross-linking of the macromolecules and dissolution of the inorganic support. particles made of roughly 80% horseradish peroxidase (hrp) as well as glucose oxidase (gox) were prepared and enzyme activities were confirmed, applying the amplex red assay. the enzyme particles were reusable for at least six times, with a remaining activity of 30-50% from the initial one. when multiple co ... | 2010 | 20486658 |
| protein radical formation resulting from eosinophil peroxidase-catalyzed oxidation of sulfite. | eosinophil peroxidase (epo) is an abundant heme protein in eosinophils that catalyzes the formation of cytotoxic oxidants implicated in asthma, allergic inflammatory disorders, and cancer. it is known that some proteins with peroxidase activity (horseradish peroxidase and prostaglandin hydroperoxidase) can catalyze oxidation of bisulfite (hydrated sulfur dioxide), leading to the formation of sulfur trioxide anion radical ((.)so(3)(-)). this free radical further reacts with oxygen to form peroxym ... | 2010 | 20501663 |
| enzyme biosensor based on the immobilization of hrp on sio₂/bsa/au composite nanoparticles. | in this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (hrp) on sio₂/bsa/au/thionine/nafion-modified gold electrode was fabricated successfully. firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (thi) as an electron mediator via the ion-exchange interaction between the thi and nafion. subsequently, the sio₂/bsa/au composite nanoparticles were assembled onto thi film through the covalent bo ... | 2010 | 20532672 |
| connections between the zona incerta of the dog diencephalon and the substantia nigra, ventral tegmental field, and pedunculopontine tegmental nucleus. | studies using technique based on retrograde and anterograde transport of horseradish peroxidase were performed to address the organization of the connections of different sectors of the zone incerta (zi) of the diencephalon with substructures of the substantia nigra, ventral tegmental field, and pedunculopontine nucleus of the midbrain in dogs. these structures were found to be interconnected. the organization of these connections included elements demonstrating that segregated conduction of fun ... | 2010 | 20532829 |
| [treatment of spinal cord injury by mild hypothermia combined with bone marrow mesenchymal stem cells transplantation in rats]. | bone marrow mesenchymal stem cells (bmscs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on bmscs transplantation, proliferation, and differentiation. to investigate the effect of bmscs transplantation and mild hypothermia on repair of rat spinal cord injury (sci). | 2010 | 20695375 |
| [ontogenetic specifics of organisation of the cortico-cortical connections of the cat primary visual cortex and lateral suprasylvian area]. | a quantitative and qualitative analysis of distribution of initial cells that organize the cortico-cortical connections between area 17 and posterior-medial lateral suprasylvian area (pmls) was carried out in 10 kittens (5- and 12-week old). a horseradish peroxidase labeling technique was used. pmls area is the highest center of moving visual stimuli information processing. certain ontogenetic peculiarities of the area 17 initial neurons regular (clustered) organization were revealed. an age-rel ... | 2010 | 20535992 |
| [immortalized mouse brain endothelial cell line bend.3 displays the comparative barrier characteristics as the primary brain microvascular endothelial cells]. | the purpose of this study was to assess weather the immortalized mouse brain endothelial cell line bend.3 displays the comparative barrier characteristics as the primary brain microvascular endothelial cells (bemc). | 2010 | 20540860 |
| methods for detection and measurement of hydrogen peroxide inside and outside of cells. | hydrogen peroxide (h(2)o(2)) is an incompletely reduced metabolite of oxygen that has a diverse array of physiological and pathological effects within living cells depending on the extent, timing, and location of its production. characterization of the cellular functions of h(2)o(2) requires measurement of its concentration selectively in the presence of other oxygen metabolites and with spatial and temporal fidelity in live cells. for the measurement of h(2)o(2) in biological fluids, several se ... | 2010 | 20526816 |
| nano-molar level hydrogen peroxide detection by horseradish peroxidase adsorbed cup-stacked carbon nanotube electrodes and applications to l-glutamate detection. | we have developed a simple fabrication method of a highly sensitive direct electron transfer-type electrochemical biosensor for hydrogen peroxide by use of cup-stacked carbon nanotubes (cscnts). the cscnts, formed by stacking of cup-shaped carbon units, has larger internal space and more hydrophilic edges, thanks to the presence of functional groups containing oxygen (e.g., -cooh, -oh), than multi-walled carbon nanotubes (mwcnts). when the cscnts suspension was cast, the cscnts were dispersed ho ... | 2010 | 20543499 |
| protein modification with amphiphilic block copoly(2-oxazoline)s as a new platform for enhanced cellular delivery. | several homopolymers, random copolymers and block copolymers based on poly(2-oxazoline)s (pox) were synthesized and conjugated to horseradish peroxidase (hrp) using biodegradable and nonbiodegradable linkers. these conjugates were characterized by amino group titration, polyacrylamide gel electrophoresis (page), isoelectric focusing, enzymatic activity assay and conformation analysis. the conjugates contained on average from about one to two polymer chains per enzyme. from 70% to 90% of enzymati ... | 2010 | 20550191 |
| activity-dependent bulk endocytosis and clathrin-dependent endocytosis replenish specific synaptic vesicle pools in central nerve terminals. | multiple synaptic vesicle (sv) retrieval modes exist in central nerve terminals to maintain a continual supply of svs for neurotransmission. two such modes are clathrin-mediated endocytosis (cme), which is dominant during mild neuronal activity, and activity-dependent bulk endocytosis (adbe), which is dominant during intense neuronal activity. however, little is known about how activation of these sv retrieval modes impact the replenishment of the total sv recycling pool and the pools that resid ... | 2010 | 20554865 |
| prognostic value of mucin 4 expression in colorectal adenocarcinomas. | mucin 4 (muc4) is aberrantly expressed in colorectal adenocarcinomas (crcs) but its prognostic value is unknown. | 2010 | 20564074 |
| igg2 dominancy and carbohydrate recognition specificity of c3h/he mouse antibodies directed to cross-reactive carbohydrate determinants (ccds) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose. | few common carbohydrate epitopes consisting of terminal beta-(1,2)-xylose and/or alpha-(1,3)-fucose residues are shared by a variety of glycoproteins from plants, insects and parasitic worms, termed cross-reactive carbohydrate determinant (ccd), and frequently recognized by ige antibodies of patients with food and/or respiratory allergy, though clinical relevancy of such ccd-specific ige is still controversial. attention has also been focused on ccds from the undesired post-translational modific ... | 2010 | 20600324 |
| real-time monitoring and kinetic parameter estimation of the affinity interaction of jartinm and rartinm with peroxidase glycoprotein by the electrogravimetric technique. | artinm is a d-mannose binding lectin that has been arousing increasing interest because of its biomedical properties, especially those involving the stimulation of th1 immune response, which confers protection against intracellular pathogens. the potential pharmaceutical applications of artinm have motivated the production of its recombinant form (rartinm) so that it is important to compare the sugar-binding properties of jartinm and rartinm in order to take better advantage of the potential app ... | 2010 | 20605432 |
| noncompetitive immunoassay for follicle-stimulating hormone in human serum using capillary electrophoresis with chemiluminescence detection. | a noncompetitive immunoassay based on capillary electrophoresis (ce) with chemiluminescence (cl) detection has been developed for the determination of follicle-stimulating hormone (fsh) in human serum. the work involved the development of separation and cl conditions allowing for routine analysis of serum samples. in this study, horseradish peroxidase (hrp)-labeled monoclonal anti-fsh can catalyze the luminol-hydrogen peroxide reaction. the determined fsh can react with an excessive amount of hr ... | 2010 | 20623406 |
| montelukast treatment (cysteinyl leukotriene receptor antagonist) in a model of food allergy: modifications in lymphatic cell population from rectal mucosa. | the aim is to determine immunopathological modifications in rectal mucosa from rabbits after local challenge in ovalbumin (ova) sensitized animals previously treated with montelukast. | 2010 | 20617862 |
| single-cell detection using a thin film transistor photosensor with micro-partitions. | a thin film transistor (tft) photosensor was applied to single-cell detection by identifying cell surface molecules based on chemiluminescence. micro-partitions were directly fabricated on the tft photosensor surface by photolithography. the surface of each pixel was surrounded by 25 μm-height partitions, forming areas of approximately 30 μm × 30 μm for cell entrapment and photosensing. visualization of individual jm cells, stained with mouse anti-human cd8 igg1 primary antibody and horseradish ... | 2010 | 20694269 |
| detection of quorum sensing molecules in burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays. | the burkholderia cepacia complex (bcc) employs a quorum sensing (qs) mechanism which is a cell density-dependent bacterial communication system to regulate certain gene expressions. as with many other gram-negative bacteria, burkholderia cepacia species use (n-acyl-)homoserine lactones (ahls or hsls) as signalling molecules. because of the essential role of qs in bacterial behavior, the aim of this study was to demonstrate the applicability of our in-house-developed enzyme-linked immunosorbent a ... | 2010 | 20694722 |
| a novel homogeneous biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum. | electrochemiluminescence (ecl) assays have been widely used for the detection of anti-therapeutic antibodies (atas) against biotherapeutics. with the discontinuation of bioveris (bv) ecl platform, an alternative technology was needed to replace bv assays to ensure continuous support of multi-year clinical studies. after evaluation of several immunoassay platforms, a novel homogeneous biotin-digoxigenin (dig) based bridging elisa format was selected to develop an anti-rhumabx antibody screening a ... | 2010 | 20868690 |
| antibody-targeted horseradish peroxidase associated with indole-3-acetic acid induces apoptosis in vitro in hematological malignancies. | indole-3-acetic acid (iaa), when oxidized by horseradish peroxidase (hrp), is transformed into cytotoxic molecules capable of inducing cell injury. the aim of this study was to test if, by targeting hematopoietic tumors with hrp-conjugated antibodies in association with iaa treatment, there is induction of apoptosis. we used two lineages of hematologic tumors: nb4, derived from acute promyelocytic leukemia (apl) and granta-519 from mantle cell lymphoma (mcl). we also tested cells from 12 patient ... | 2010 | 21168913 |
| ultrasensitive electrochemical immunoassay of staphylococcal enterotoxin b in food using enzyme-nanosilica-doped carbon nanotubes for signal amplification. | a new sandwich-type electrochemical immunoassay for ultrasensitive detection of staphylococcal enterotoxin b (seb) in food was developed using horseradish peroxidase-nanosilica-doped multiwalled carbon nanotubes (hrpsicnts) for signal amplification. rabbit polyclonal anti-seb antibodies immobilized on the screen-printed carbon electrode (spce) and covalently bound to the hrpsicnts were used as capture antibodies and detection antibodies, respectively. in the presence of seb analyte, the sandwich ... | 2010 | 20873791 |
| immunosensor based on fluorescence quenching matrix of the conducting polymer polypyrrole. | in this study, the combination of autofluorescent proteins and fluorescence quenching polymers was shown to be a design which can increase the selectivity and sensitivity of immunosensors. with this objective, the conducting polymer polypyrrole (ppy) was used as a matrix for immobilization of proteins, which enables biological recognition of the analyte, and as a fluorescence quencher, which increases the selectivity of fluorescence-based detection. in this study, bovine leukemia virus proteins ... | 2010 | 20941482 |
| a peptide-based material platform for displaying antibodies to engage t cells. | this study investigated a strategy by which antibodies are displayed to engage t cells. a peptide composite containing binding sites was characterized in vitro and explored as an injectable system in vivo. the composite consists of two amphiphilic peptides, aeaeakakaeaeakak (referred to as "eak") and eak appended with six consecutive histidines at the c-terminus ("eakh6"). spectroscopic analysis showed the two peptides integrated into a single structure. prior to combination, conformational anal ... | 2010 | 20880580 |
| simultaneous detection of multiple chemical residues in milk using broad-specificity antibodies in a hybrid immunosorbent assay. | in this study, a novel immunoassay using 2 types of sensors (qds and an enzyme) were simultaneously used for detecting multiple structurally different molecules in milk. the method integrates the fluorescence-linked immunosorbent assay (flisa) using qd605 and qd655 as probes and an enzyme-linked immunosorbent assay (elisa) using horseradish peroxidase (hrp) labeled secondary antibody. the flisa was produced by anti-sulfonamide and anti-quinolone broad-specificity monoclonal antibodies (mabs) for ... | 2010 | 20947327 |
| microfluidic immunosensor with gold nanoparticle platform for the determination of immunoglobulin g anti-echinococcus granulosus antibodies. | this article describes a microfluidic immunosensor, developed for the detection of igg antibodies specific to echinococcus granulosus in human serum samples, which represents an alternative tool that can be used for the immunodiagnosis of hydatidosis in an automated way. our device consists of a plexiglas system with a central channel and a gold electrode. for immobilization of the e. granulosus antigen, a gold electrode was modified with the incorporation of gold nanoparticles. immobilized anti ... | 2010 | 20951112 |
| a simple and rapid competitive enzyme-linked immunosorbent assay (celisa) for high-throughput measurement of secretory immunoglobulin a (siga) in saliva. | a simple competitive enzyme-linked immunosorbent assay (celisa) was established for rapid measurement of secretory immunoglobulin a (siga) in saliva. the method was based on competitive reaction between the immobilized iga and free iga in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human iga. in comparison with the conventionally used sandwich elisa, the celisa is simpler, low-cost, and shows better reproducibility since it is not affected by the variatio ... | 2010 | 20685490 |
| ternary surface monolayers for ultrasensitive (zeptomole) amperometric detection of nucleic acid hybridization without signal amplification. | a ternary surface monolayer, consisting of coassembled thiolated capture probe, mercaptohexanol and dithiothreitol, is shown to offer dramatic improvements in the signal-to-noise characteristics of electrochemical dna hybridization biosensors based on common self-assembled monolayers. remarkably low detection limits down to 40 zmol (in 4 μl samples) as well as only 1 cfu escherichia coli per sensor are thus obtained without any additional amplification step in connection to the commonly used hor ... | 2010 | 20883023 |
| increasing of blood-tumor barrier permeability through paracellular pathway by low-frequency ultrasound irradiation in vitro. | the research was conducted to study the increase of blood-tumor barrier (btb) permeability through paracellular pathway by low-frequency ultrasound (lfu) irradiation in vitro. lfu (frequency=1.0 mhz) was performed to irradiate btb model from the co-culture of rat c6 glioma cells and rat brain microvascular endothelial cells (rbmecs). the permeability of btb was measured by transendothelial electrical resistance (teer) and flux of horseradish peroxidase (hrp) assays after lfu irradiation. western ... | 2010 | 21104456 |
| feeding on poplar leaves by caterpillars potentiates foliar peroxidase action in their guts and increases plant resistance. | peroxidases (pods) are believed to act as induced and constitutive defenses in plants against leaf-feeding insects. however, little work has examined the mode of action of pods against insects. putative mechanisms include the production of potentially antinutritive and/or toxic semiquinone free radicals and quinones (from the oxidation of phenolics), as well as increased leaf toughness. in this study, transgenic hybrid poplar saplings (populus tremula × populus alba) overexpressing horseradish p ... | 2010 | 20680646 |
| rapid quantification of melamine in milk using competitive 1,1'-oxalyldiimidazole chemiluminescent enzyme immunoassay. | a novel competitive 1,1'-oxalyldiimidazole (odi) chemiluminescent enzyme immunoassay (cleia) was developed as a method for rapid and simple screening of melamine in milk. fat existing in milk acts as an inhibitor in the competitive binding interaction of melamine and anti-melamine in the presence of melamine-conjugated horseradish peroxidase. thus, the calibration curve and sensitivity of competitive odi cleia for the quantification of melamine in fat free milk were wider and better than those i ... | 2010 | 20683522 |
| in situ electrochemical imaging of membrane glycan expression on micropatterned adherent single cells. | a scanning electrochemical microscopic (secm) method for in situ imaging of four types of membrane glycan motifs on single adherent cells was proposed using bgc-823 human gastric carcinoma (bgc) cells as the model. these adherent cells were first micropatterned in the microwell of poly(dimethylsiloxane) membrane for precisely controlling the localized surface interaction, and the membrane glycans were then specifically recognized with corresponding lectins labeled with horseradish peroxidase (hr ... | 2010 | 20684525 |
| stopped-flow microarray immunoassay for detection of viable e. coli by use of chemiluminescence flow-through microarrays. | rapid multiplexed analysis of microorganisms is important in water analysis to control bacterial contamination for health and safety reasons. direct quantification of bacteria by means of flow-through microarray immunoassays requires new analysis strategies for optimising sensitivity and the analysis time. for bacteria and for particles, hydrodynamic forces and sedimentation are the dominating effects for binding on surfaces in a flow-through system, whereas diffusion is insignificant. therefore ... | 2010 | 21107820 |
| kinetic analysis of phagosomal production of reactive oxygen species. | phagocytes produce large quantities of reactive oxygen species for pathogen killing; however, the kinetics and amplitude of ros production on the level of individual phagosomes are poorly understood. this is mainly due to the lack of appropriate methods for quantitative ros detection with microscopic resolution. we covalently attached the ros-sensitive dye dichlorodihydrofluorescein (dcfh(2)) to yeast particles and investigated their fluorescence due to oxidation in vitro and in live phagocytes. ... | 2010 | 21111807 |
| enzyme linked immunosorbent assay for milk progesterone. | a direct antigen heterologous enzyme linked immunosorbent assay (elisa) for milk progesterone has been developed using progesterone-3-o-carboxymethyloxime-bovine serum albumin (p-3-o-cmo-bsa) antiserum and 17-α-hydroxy-progesterone-3-o-carboxymethyloxime-horseradish peroxidase (17-α-oh-p-3-o-cmo-hrp) enzyme conjugate. the data of the present study reveal that the homologous assay, which employed p-3-o-cmo-hrp as the label, showed no displacement. on the contrary, replacement of p-3-o-cmo-hrp wit ... | 2010 | 21113843 |
| cation exchange in znse nanocrystals for signal amplification in bioassays. | znse nanocrystals (ncs), possessing low native luminescence but high biocompatibility, were employed as labeling tags in bioassays. they were able to amplify each target recognition event thousands of times through a cation-exchange reaction (cxamp) that released over 3000 encapsulated zn(2+) from one single nc. the freed cations in turn triggered strong fluorescence from the zn-responsive dyes. the present study demonstrated that cxamp with znse delivered superior detection performance in compa ... | 2010 | 21117624 |
| the expression of nadph oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line a549. | controlled production of reactive oxygen species (ros) by nadph oxidases in non-phagocytic cells has recently been suggested to participate in the regulation of cellular functions. due to the role of ros in control of cellular functions, precise and accurate detection of ros is of essential importance. however, various methodological approaches currently used for ros determination vary in sensitivity, specificity, as well as in requirements for specialized equipment. in this study, human lung ep ... | 2010 | 21138653 |
| quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification with self-produced coreactant from oxygen reduction. | a highly sensitive competitive immunosensor based on the electrochemiluminescence (ecl) of quantum dots (qds) was proposed by coupling with an enzymatic amplification. the fabrication process of the immunosensor was traced with atomic force microscopic images and electrochemical impedance spectra. the strong cathodic ecl emission of the immobilized qds could be detected at a relatively low emission potential. the reduction of dissolved oxygen during the cathodic process provided a self-produced ... | 2010 | 20695454 |
| immobilization of horseradish peroxidase on nanoporous copper and its potential applications. | nanoporous copper (npc) with a pore size of 100-200 nm was prepared by simply dealloying al(60)cu(40) alloy in a 5 wt.% hcl solution. the npc was characterized by scanning electron microscopy and nitrogen adsorption techniques. horseradish peroxidase (hrp) was immobilized on npc by adsorption. compared with free enzyme, the thermal stability of the immobilized enzyme was greatly improved due to the multiple attachments between the enzyme molecule and the npc surface. after 2h incubation at 50 de ... | 2010 | 20709542 |
| the role of stiffness of gelatin-hydroxyphenylpropionic acid hydrogels formed by enzyme-mediated crosslinking on the differentiation of human mesenchymal stem cell. | we report the stimulation of neurogenesis and myogenesis of human mesenchymal stem cells (hmscs) on the surfaces of biodegradable hydrogels with different stiffness. the hydrogels were composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugate were formed using the oxidative coupling of phenol moieties catalyzed by hydrogen peroxide (h(2)o(2)) and horseradish peroxidase (hrp). the storage modulus of the hydrogels was readily tuned from 600 to 12800 pa. it was found that the stiffness of ... | 2010 | 20709390 |
| oxidative metabolism of the anti-cancer agent mitoxantrone by horseradish, lacto-and lignin peroxidase. | mitoxantrone (mh(2)x), an anthraquinone-type anti-cancer agent used clinically in the treatment of human malignancies, is oxidatively activated by the peroxidase/h(2)o(2) enzyme system. in contrast to the enzymatic mechanisms of drug oxidation, the chemical transformations of mh(2)x are not well described. in this study, mh(2)x metabolites, produced by the horseradish, lacto- or lignin peroxidase (respectively hrp, lpo and lip)/h(2)o(2) system, were investigated by steady-state spectrokinetic an ... | 2010 | 20887767 |
| oxidation reactions mediated by single-walled carbon nanotubes in aqueous solution. | how single-walled carbon nanotubes (swcnt) function in redox reactions may be related to their behaviors in the induction of oxidative stress. herein, oxidation of several biologically relevant reducing agents in the presence of swcnt was studied in aqueous solutions. the selected reductants included a common indicator for intracellular reactive oxygen species (ros) (2,7-dichlorodihydrofluorescein), small antioxidants (vitamin c, trolox, and cysteine), and a high-molecular-weight ros scavenger ( ... | 2010 | 20715868 |
| a secondary antibody format chemiluminescence immunoassay for the determination of estradiol in human serum. | a competitive immunoassay for estradiol (e2) based on secondary antibody format was established. the donkey anti-rabbit igg was used as the secondary antibody to coat micro-plates, and the horseradish peroxidase (hrp)-luminol-h(2)o(2) chemiluminescent system with high sensitivity was chosen as the detection system. the addition of sodium trichloroacetate (ccl(3)coona) in the enzyme buffer as a replaceable packing material can realize directly analysis of e2 in human serum without extraction, whi ... | 2010 | 20801358 |
| advent of innovative chemiluminescent enzyme immunoassay. | using 1,1'-oxalyldiimidazole (odi) chemiluminescence detection, a new chemiluminescent enzyme immunoassay (cleia) was developed to quantify prostate specific antigen (psa) in human serum. the results observed in odi cleia were compared with those obtained in commercially available enzyme linked immunosorbent assay (elisa), fluorescence enzyme immunoassay (feia), and luminol cleia. psa complex-conjugated horseradish peroxidase (hrp) was formed from one-step sandwich immunoreaction of psa, psa pri ... | 2010 | 20739174 |
| qsar-assisted design of an environmental catalyst for enhanced estrogen remediation. | a quantitative structure-activity relationship (qsar) was used to streamline re-design of a model environmental catalyst, horseradish peroxidase (hrp), for enhanced reactivity towards a target pollutant, steroid hormone 17β-estradiol. this qsar, embodying relationship between reaction rate and intermolecular binding distance, was used in silico to screen for mutations improving enzyme reactivity. eight mutations mediating significant reductions in binding distances were expressed in saccharomyce ... | 2010 | 20797763 |
| polystyrene microspheres based sandwich immunosensor using cdte nanoparticles amplification and ultrasensitive flow-injection chemiluminescence detection. | in this paper we propose a specific sandwich immunoassay method for human-immunoglobulin g (higg). this immunoassay protocol takes advantage of sandwich binding of primary and secondary antibodies for increased specificity. polystyrene microspheres (ps) serve as immobilizing support, site for sandwich immunoassay and then subsequently used for chemiluminescence (cl) detections. in this sandwich immunoassay, ps microspheres were modified with the primary anti-higg (ab1) via electrostatic interact ... | 2010 | 20801005 |
| in vitro studies of dna damage caused by tricyclic antidepressants: a role of peroxidase in the side effects of the drugs. | studies show that tricyclic antidepressants prescribed for migraines, anxiety, and child enuresis have numerous adverse effects in living cells. one of the undesired outcomes observed under treatment with these drugs is dna damage. however, the mechanisms underlying damage have yet to be elucidated. we performed in vitro studies of the dna damage caused by four tricyclic antidepressants: imipramine, amitriptyline, opipramol, and protriptyline. we focused particularly on the dna damage aided by p ... | 2010 | 20804147 |
| lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen cryopreserved in extenders with antioxidants. | the objective of this study was to evaluate the effect of addition of the antioxidants trolox and catalase to a ram semen cryopreservation extender on lipid peroxidation and hydrogen peroxide generation on the extender and in the thawed semen. semen was collected from 23 santa inês rams (one ejaculate per ram) and diluted at 32°c to a concentration of 400×10⁶ cells/ml in one of the following solution: tris-egg yolk extender (control), or the same extender supplemented with either 50μm trolox/10⁸ ... | 2010 | 20813469 |
| a selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules. | a new assay system for chitin has been developed. it comprises the chitin-binding protein chbb in fusion with a his-tag as well as with a strep-tag, the latter of which was chemically coupled to horseradish peroxidase. with the resulting complex, minimal quantities of chitin are photometrically detectable. in addition, the assay allows rapid scoring of the activity of chitin-synthases. as a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin bi ... | 2010 | 20957083 |
| tanshinone iia protects the human blood-brain barrier model from leukocyte-associated hypoxia-reoxygenation injury. | to investigate the in vitro effect of tanshinone iia on leukocyte-associated hypoxia-reoxygenation injury of human brain-blood barrier (bbb), we established the bbb model by culturing purified primary human brain microvascular endothelial cells (hbmvec) to confluence on cell culture insert. bbb was identified by tight junction, transendothelial electrical resistance (teer) and the permeability of bbb to horseradish peroxidase (hrp). the effect of tanshinone iia on the permeability of bbb was tes ... | 2010 | 20826144 |
| fluorometric assay of oleate-activated phospholipase d isoenzyme in membranes of rat nervous tissue and human platelets. | phospholipase d plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. oleate-activated phospholipase d was the first mammalian phospholipase d isoform to be discovered but is the least known. the study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase d activity in different biological materials. the brain cortex of male wistar rats, cultured rat brain astrocytes, and human platelets were proc ... | 2010 | 20835407 |
| comparison of enzyme-linked immunosorbent assay-based techniques for the detection of antibody to rift valley fever virus in thermochemically inactivated sheep sera. | different enzyme-linked immunosorbent assay (elisa)-based techniques for the detection of antibodies to rift valley fever virus (rvfv) have been developed in recent years, but their diagnostic sensitivity was not directly compared. in addition, their use might still be restricted to high biocontainment facilities when sera to be tested are collected from viremic individuals. in this study, we report on direct comparison of various elisa forms for the detection of anti-rvfv antibody in preinactiv ... | 2010 | 20854023 |
| visualization of microvascular blood flow in mouse kidney and spleen by quantum dot injection with "in vivo cryotechnique". | the "in vivo cryotechnique" (ivct) is a powerful tool to instantly capture blood flow, and all plasma components are well kept in tissue samples. in this study, we injected glutathione (gsh)-coated quantum dots (qds), which emit a 650-nm-fluorescent signal with an ultraviolet excitation, into anesthetized mouse left ventricles, and ivct was performed for kidneys, spleens and livers at 2, 5, 10, 15, 30s or 24h after the qd injection. the frozen tissues were processed to freeze-substitution fixati ... | 2010 | 20858507 |
| prognostic significance of cyclin a and b1 in pediatric embryonal tumors. | embryonal tumors constitute the most common malignant brain tumor group in children. although patient prognosis has been substantially improved over recent decades, identification of prognostic markers would be of obvious significance. in the present study we evaluated the prognostic significance of cyclin a and b1 in correlation with ki-67 index in pediatric embryonal tumors. we retrospectively evaluated 53 children with embryonic tumors who were treated surgically in our institute. all patient ... | 2010 | 21069428 |
| direct projections of omnipause neurons to reticulospinal neurons: a double-labeling light microscopic study in the cat. | omnipause neurons (opns) are inhibitory neurons located in the midline region of the caudal pons. their role in gating the discharges of saccade-related burst neurons is well known, but there is no agreement concerning their influence on brainstem neurons that control other muscle groups participating in rapid gaze shifts. in the present study, we inquired whether opns project directly to pontobulbar reticulospinal neurons (rsns) in the cat. retrograde transport of horseradish peroxidase from th ... | 2010 | 20963829 |
| ultrasensitive electrochemical aptasensor for thrombin based on the amplification of aptamer-aunps-hrp conjugates. | successful development of an ultrasensitive and highly specific electrochemical aptasensor for thrombin based on amplification of aptamer-gold nanoparticles-horseradish peroxidase (aptamer-aunps-hrp) conjugates was reported. in this electrochemical protocol, aptamer1 (apt1) was immobilized on core/shell fe(3)o(4)/au magnetic nanoparticles (aumnps) and served as capture probe. aptamer2 (apt2) was dual labeled with aunps and hrp and used as detection probe. in the presence of thrombin, the sandwic ... | 2010 | 21030239 |
| nutritive evaluation and effect of moringa oleifera pod on clastogenic potential in the mouse. | moringa oleifera lam (horseradish tree; tender pod or fruits) has been consumed as a vegetable and utilized as a major ingredient of healthy thai cuisine. previous studies have shown that m. oleifera pod extracts act as bifunctional inducers along with displaying antioxidant properties and also inhibiting skin papillomagenesis in mice. this study was aimed to determine the nutritive value, and clastogenic and anticlastogenic potentials of m. oleifera pod. the nutritive value was determined accor ... | 2010 | 21039028 |
| potential of melatonin to treat or prevent age-related macular degeneration through stimulation of telomerase activity. | melatonin may play a causal role in the occurrence of age-related macular degeneration (amd). replicative capacity and response to injury in the retinal pigment epithelium (rpe) is compromised during aging. prevention of telomere shortening by antioxidants may be a useful approach for reducing the cumulative effects of oxidative stress in rpe cells. melatonin, a well known antioxidant, which acts advantageously as an amphiphilic agent, may benefit amd patients more than commonly used lipophilic ... | 2010 | 20884126 |
| tresyl-based conjugation of protein antigen to lipid nanoparticles increases antigen immunogenicity. | the present studies were aimed at investigating the engineering of nps with protein-conjugated-surfactant at their surface. in order to increase the immunogenicity of a protein antigen, brij 78 was functionalized by tresyl chloride and then further reacted with the primary amine of the model proteins ovalbumin (ova) or horseradish peroxide (hrp). the reaction yielded brij 78-ova and brij 78-hrp conjugates which were then used directly to form np-ova or np-hrp using a one-step warm oil-in-water m ... | 2010 | 20837122 |
| resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells. | a large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. the cells were identified as perivascular resident macrophages (pvms), as they were positive for several macrophage surface molecules including f4/80, cd68, and cd11b. the macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor ... | 2010 | 20838812 |
| development of ultra-sensitive soybean peroxidase-based cl-elisa for the determination of human thyroglobulin. | serum thyroglobulin (tg) is a main marker of thyroid cancer relapses after total or near-total thyroidectomy of patients with differentiated thyroid carcinoma. in this study, we developed a chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) for detecting tg in human serum. soybean peroxidase (sbp) in combination with 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) and 4-morpholinopyridine (morph) and horseradish peroxidase (hrp) with p-iodophenol (pip) were used as detection systems ... | 2010 | 20851124 |
| increased plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain. | pmd (pelizaeus-merzbacher disease) is a rare neurodegenerative disorder that impairs motor and cognitive functions and is associated with a shortened lifespan. the cause of pmd is mutations of the plp1 [proteolipid protein 1 gene (human)] gene. transgenic mice with increased plp1 [proteolipid protein 1 gene (non-human)] copy number model most aspects of pmd patients with duplications. hypomyelination and demyelination are believed to cause the neurological abnormalities in mammals with plp1 dupl ... | 2010 | 20885931 |
| one-chip biosensor for simultaneous disease marker/calibration substance measurement in human urine by electrochemical surface plasmon resonance method. | we have developed a miniaturized electrochemical surface plasmon resonance biosensor for measuring two biomolecules that have very different molecular sizes, one is transferrin (mw=75 kda) as a disease marker protein, the other is creatinine (mw=113) as a calibration marker for the accurate measurement of human urinary samples. the sensor has a pdms based microchannel that is 2 mm wide and 20 μm deep. two gold films were integrated in the microchannel; one was modified with anti-transferrin anti ... | 2010 | 20888212 |
| nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum. | a new electrochemical immunoassay protocol for the determination of carcinoembryonic antigen (cea) in human serum was developed by means of immobilizing horseradish peroxidase-labeled anti-cea antibodies (hrp-anti-cea) on the nanogold/nano-fe(3)o(4) particles-functionalized polyion complex (pic) membrane. with a one-step immunoassay format, the formed immunocomplex inhibited partly the bioactive center of the immobilized hrp, and decreased the hrp toward the reduction of h(2)o(2). nanogold-actua ... | 2010 | 20888448 |
| gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimer for sensitive electrochemical immunoassay of brevetoxins in food samples. | a sensitive electrochemical immunosensor for the fast screening of brevetoxin b (btx-2) in food samples was developed by means of immobilizing btx-2-bovine serum albumin conjugate (btx-2-bsa) on the gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimers (aunp-paads). the presence of gold nanoparticles greatly improved the conductivity of the paads, and three-dimensional paads increased the surface coverage of the biomolecules on the electrode. under optimal conditions, three t ... | 2010 | 20926280 |
| peripheral nerve injury and trpv1-expressing primary afferent c-fibers cause opening of the blood-brain barrier. | the blood-brain barrier (bbb) plays the crucial role of limiting exposure of the central nervous system (cns) to damaging molecules and cells. dysfunction of the bbb is critical in a broad range of cns disorders including neurodegeneration, inflammatory or traumatic injury to the cns, and stroke. in peripheral tissues, the vascular-tissue permeability is normally greater than bbb permeability, but vascular leakage can be induced by efferent discharge activity in primary sensory neurons leading t ... | 2010 | 21044346 |
| high sensitivity dna detection using gold nanoparticle functionalised polyaniline nanofibres. | polyaniline (pani) nanofibres (pani-nf) have been modified with chemically grown gold nanoparticles to give a nanocomposite material (pani-nf-aunp) and deposited on gold electrodes. single stranded capture dna was then bound to the gold nanoparticles and the underlying gold electrode and allowed to hybridise with a complementary target strand that is uniquely associated with the pathogen, staphylococcus aureus (s. aureus), that causes mastitis. significantly, cyclic voltammetry demonstrates that ... | 2010 | 21159503 |
| projections from the brain to the spinal cord in the mouse. | the cells that project from the brain to the spinal cord have previously been mapped in a wide range of mammalian species, but have not been comprehensively studied in the mouse. we have mapped these cells in the mouse using retrograde tracing after large unilateral fluoro-gold (fg) and horseradish peroxidase (hrp) injections in the c1 and c2 spinal cord segments. we have identified over 30 cell groups that project to the spinal cord, and have confirmed that the pattern of major projections from ... | 2010 | 20936329 |
| [demonstration of afferent connections of the forebrain in emys orbicularis turtles by the peroxidase method]. | the transport of horseradish peroxidase (hrp) out of the injection site in the dorsal ventricular ridge was studied in turtles emys orbicularis. labeled cells in the forebrain were observed in the paleostriatum among fibers of the lateral forebrain bundle. in the thalamus most of cells containing the granular hrp reaction product were located in the n. rotundus, n. reuniens and perirotundal nuclei (n. dorso-medialis anterior, n. magnocellularis thalami, n (centralis) lateralis, n. dorso-medialis ... | 2010 | 654713 |
| [quantitative immunoenzyme determination of lactoferrin in the serum and plasma]. | a procedure for quantitative estimation of lactoferrin is developed using the "sandwich" type of immunoenzymatic assay. preparation of lactoferrin was purified from women colostrum by means of ion exchange chromatography; the sorbent (a ligand-containing complex of lactoferrin) was synthesized; the antibody conjugate against lactoferrin and peroxidase from horseradish was prepared; optimal conditions of the immunoenzymatic assay were selected. concentration of lactoferrin in donor blood serum or ... | 2011 | 2697964 |
| effects of asymmetric dimethylarginine on bovine retinal capillary endothelial cell proliferation, reactive oxygen species production, permeability, intercellular adhesion molecule-1, and occludin expression. | asymmetric dimethylarginine (adma), an endogenous competitive inhibitor of nitric oxide synthase, is associated with impaired endothelial dysfunction, such as chronic heart failure, hypertension, diabetes, and pulmonary hypertension. the effects of adma on cell proliferation, reactive oxygen species (ros) production, cell permeability, intercellular adhesion molecule-1 (icam-1), and tight-junction protein occludin levels in bovine retinal capillary endothelial cells (brcecs) were investigated. | 2011 | 21297899 |
| a disposable immunosensor for shigella flexneri based on multiwalled carbon nanotube/sodium alginate composite electrode. | a novel shigella flexneri immunosensor based on horseradish peroxidase-labeled antibodies to s. flexneri (hrp-anti-s. flexneri) immobilized by physical adsorption on the multiwalled carbon nanotube (mwcnt)/sodium alginate (sa) composite modified screen-printed electrode surface was successfully fabricated. in this strategy, mwcnt/sa biocomposite acted as the matrix to adsorb and immobilize hrp-anti-s. flexneri. the modified electrodes were characterized with an atomic force microscope and cyclic ... | 2011 | 20816661 |
| suitability of different glycoproteins and test systems for detecting cross-reactive carbohydrate determinant-specific ige in hymenoptera venom-allergic patients. | in hymenoptera venom allergy, about 75% of detected in vitro double positivity to yellow jacket and honeybee venom is ascribed to specific ige (sige) directed against cross-reactive carbohydrate determinants (ccds). to date, for the detection of ccd-sige, different carbohydrate antigens and methods are used. the most suitable one still has to be identified. | 2011 | 21447958 |
| direct retinal projections to the lateroposterior thalamic nucleus (lp) in the mole. | direct retinothalamic projections in the mole, an animal with a highly reduced visual system, were studied after an intraocular injection of wheat germ-agglutinated horseradish peroxidase (wga-hrp). anterogradely labeled axon terminals were found not only in the dorsal and ventral nuclei of the lateral geniculate body but also in the lateroposterior nucleus on the side contralateral to the intraocular wga-hrp injection. | 2011 | 3241641 |
| [ultrastructural analysis of the frog sensorimotor synapse physiologically identified and labeled with horseradish peroxidase]. | 2011 | 3032551 | |
| development of phosphonate modified fe 1-x mnxfe2o4 mixed ferrite nanoparticles: novel peroxidase mimetics in enzyme linked immunosorbent assay. | a highly facile and feasible strategy on the fabrication of advanced intrinsic peroxidase mimetics based on mn(2+) doped mixed ferrite (mn(ii)(x)fe(ii)(1-x)fe(iii)(2)o(4)) nanoparticles was demonstrated for the quantitative and sensitive detection of mouse igg (as a model analyte). mn(2+) doped fe(1-x)mn(x)fe(2)o(4) nanoparticles were synthesized using varying ratios of mn(2+):fe(2+) ions and characterized by the well known complementary techniques. the increase of mn(2+) proportion had remarkab ... | 2011 | 22063549 |
| a highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer. | a two-dimensional gaseous ethanol visualization system has been developed and demonstrated using a horseradish peroxidase-luminol-hydrogen peroxide system with high-purity luminol solution and a chemiluminescence (cl) enhancer. this system measures ethanol concentrations as intensities of cl via the luminol reaction. cl was emitted when the gaseous ethanol was injected onto an enzyme-immobilized membrane, which was employed as a screen for two-dimensional gas visualization. the average intensity ... | 2011 | 23044769 |
| urothelial plaque formation in post-golgi compartments. | urothelial plaques are specialized membrane domains in urothelial superficial (umbrella) cells, composed of highly ordered uroplakin particles. we investigated membrane compartments involved in the formation of urothelial plaques in mouse umbrella cells. the golgi apparatus did not contain uroplakins organized into plaques. in the post-golgi region, three distinct membrane compartments containing uroplakins were characterized: i) small rounded vesicles, located close to the golgi apparatus, were ... | 2011 | 21887288 |