Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [genetic transformation of somatic cells. vi. transfer of the trait of the rate of loss of transformant phenotype by means of dna from cells containing the thymidine kinase gene of the herpes simplex virus]. | using dot-hybridization with thymidine kinase gene (tk gene) of herpes simplex virus type 1 (hsv 1) of dna preparations obtained from isolated metaphase chromosomes and lysate fractions of metaphase cells, which presumably contain smaller particles compared to metaphase chromosomes, it has been shown that the tk gene of hsv 1 is localized in chromosomes of cells of transformant clones unstable in tk+-phenotype. the dna isolated from the metaphase chromosomes from cells of transformant clones is ... | 1985 | 2992137 |
| preclinical assessment of topical treatments of herpes simplex virus infection: 5% (e)-5-(2-bromovinyl)-2'-deoxyuridine cream. | the potential efficacy of topical therapy with (e)-5-w-bromovinyl)-2'-deoxyuridine (bvdu) for cutaneous herpesvirus infection was evaluated in vitro and in guinea pigs. drug sensitivity testing against herpes simplex virus type 1 strain e115 revealed an id50 of 0.008 microgram/ml for bvdu and 0.19 microgram/ml for acyclovir (acv). in vitro drug diffusion studies showed poor penetration of guinea pig skin by bvdu from the cream compared to bvdu from dimethylsulfoxide (dmso) (0.04 vs. 1.5 microgra ... | 1985 | 2992371 |
| spread of herpes simplex virus and distribution of latent infection after intraocular infection of the mouse. | intraocular inoculation of hsv 1 in the mouse results not only in uveitis, but also in the spread of virus via sensory, sympathetic and optic nerves. during the acute infection with hsv 1 strain sc 16 in both outbred and nih (inbred) mice, virus reached the ipsilateral trigeminal ganglion, superior cervical ganglion, both sides of the brain stem and the contralateral (uninoculated) eye. with hsv 1 strain kos in outbred mice the same tissues became infected but virus was also isolated from the op ... | 1985 | 2992417 |
| iontophoresis of epinephrine isomers to rabbit eyes induced hsv-1 ocular shedding. | iontophoresis of 0.01% levo(-) epinephrine for 8 min at 0.8 mamp once daily for 3 consecutive days induces ocular shedding of herpes simplex virus type 1 (hsv-1) in latently infected rabbits. in the present experiment, we tested dextro(+) and levo(-) epinephrine for their comparative effects on induced hsv-1 ocular shedding. one hundred percent of the eyes shed virus after either 0.01% dextro(+) or levo(-) epinephrine iontophoresis (8 min, 0.8 mamp). however, the shedding frequency caused by 0.0 ... | 1985 | 2993192 |
| metabolism of the carbocyclic analogue of (e)-5-(2-iodovinyl)-2'-deoxyuridine in herpes simplex virus-infected cells. incorporation of c-ivdu into dna. | the carbocyclic analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu), in which the sugar moiety is replaced by a cyclopentane ring and which have been designated as c-bvdu and c-ivdu, respectively, are, like their parent compounds bvdu and ivdu, potent and selective inhibitors of herpes simplex virus type 1 (hsv-1) and, to a lesser extent, herpes simplex virus type 2 (hsv-2) replication. we have now synthesized the radiolabeled c-ivdu analogue, ... | 1985 | 2993282 |
| the therapeutic efficacy of a xanthate compound on herpes simplex virus in skin lesions of mice and guinea-pigs. | xanthates have recently been shown to inhibit the replication of both dna and rna viruses in vitro. the antiviral activity was exerted only under acidic ph conditions. curative effects in vivo on herpes simplex virus (hsv)-induced skin lesions were only observed when the xanthate compound was administered in the form of an ointment containing acidic buffer (sodium phosphate ph 5.0). advanced hsv-2-induced skin lesions in mice were healed by topical treatment with the xanthate compound. hsv-1-ind ... | 1985 | 2993486 |
| identification of cross-reacting glycoproteins of four herpesviruses by western blotting. | monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ... | 1985 | 2993488 |
| lack of oral hsv-2 in a college student population. | a population of individuals with a high incidence of genital herpes simplex virus type 1 (hsv-1), due most likely to oro-genital contact, was examined to determine the incidence of oral herpes simplex virus type 2 (hsv-2) infection. herpes simplex virus was isolated from the oral cavity of 43 college students whose symptoms ranged from singular lesions of the lips with minimal discomfort to severe oral disease with systemic involvement resulting in lymphadenopathy, chills, sweat, myalgia, and fe ... | 1985 | 2993498 |
| synthesis and antiherpes simplex virus activity of 9-[(1,3-dihydroxy-2-propylthio)methyl]guanine. | the synthesis of the thio analogue (thio-dhpg, 2) of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) is described. the synthesis of 2 proceeded via the condensation of acetoxymethyl sulfide 9 with diacetylguanine 10 to give the protected nucleoside analogue 11. although catalytic hydrogenolysis failed, the benzyl ether functionalities of 11 were successfully cleaved by an acetolysis reaction to furnish 14. ammonolysis of 14 gave 2, which was also transformed to sulfoxide 15 and sulfone 16. ... | 1985 | 2993615 |
| sialylated oligosaccharides o-glycosidically linked to glycoprotein c from herpes simplex virus type 1. | glycoprotein c (gc) was purified by immunoabsorbent from herpes simplex virus type-1-infected bhk cells labeled with [14c]glucosamine for 11 h and chased for 3 h. glycopeptides obtained by pronase digestion of gc were fractionated by bio-gel filtration and concanavalin a-sepharose chromatography. each glycopeptide fraction was analyzed for amino sugar composition by thin-layer chromatography. the majority of radioactivity was recovered as n-acetylglucosamine, but a significant amount of labeled ... | 1985 | 2993643 |
| regulation of cytomegalovirus gene expression: alpha and beta promoters are trans activated by viral functions in permissive human fibroblasts. | we have fused immediate (alpha) and delayed (beta) early promoter-regulatory sequences taken from the cytomegalovirus (cmv) genome to escherichia coli lacz (beta-galactosidase) as an indicator gene to study regulated expression of these promoters. after transfection of human fibroblast cells with plasmid constructs carrying beta-galactosidase fusions, and subsequent infection with cmv, we have demonstrated that viral trans-acting functions up-regulate the expression of these genes in a temporall ... | 1985 | 2993644 |
| virus-induced modification of the host cell is required for expression of the bacterial chloramphenicol acetyltransferase gene controlled by a late herpes simplex virus promoter (vp5). | the requirements for expression of genes under the control of early (alkaline exonuclease) and late (vp5) herpes simplex virus type 1 (hsv-1) gene promoters were examined in a transient expression assay, using the bacterial chloramphenicol acetyltransferase gene as an expression marker. both promoters were induced, resulting in the production of high levels of the enzyme upon low-multiplicity infection by hsv-1. s1 nuclease analysis of hybrids between rna isolated from infected cells containing ... | 1985 | 2993649 |
| application of antibody to synthetic peptides for characterization of the intact and truncated alpha 22 protein specified by herpes simplex virus 1 and the r325 alpha 22- deletion mutant. | the alpha 22 protein is one of five proteins synthesized immediately after infection of permissive cells with herpes simplex virus 1 and 2 (hsv-1 and hsv-2). on the basis of the reported nucleotide sequence of the hsv-1 gene, we synthesized two peptides containing the predicted amino acids 12 through 23 (12 residues) and 21 through 36 (16 residues) in two hydrophilic domains near the n terminus of the protein. rabbit antisera made against these peptides were then used to characterize the alpha 2 ... | 1985 | 2993651 |
| virion component of herpes simplex virus type 1 kos interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186. | herpes simplex virus (hsv) strains hsv type 1 (hsv-1) kos and hsv-2 186 are representative of delayed and early shutoff strains, respectively, with regard to their ability to inhibit protein synthesis in friend erythroleukemia cells. when these cells were simultaneously infected with hsv-1 kos and hsv-2 186, hsv-1 kos interfered with the rapid suppression of globin synthesis induced by hsv-2 186. the observed interference was competitive and not due to exclusion of hsv-2 by hsv-1 at the level of ... | 1985 | 2993660 |
| sp1 binds to promoter sequences and activates herpes simplex virus 'immediate-early' gene transcription in vitro. | during a herpes simplex virus (hsv-1) lytic infection, three classes of viral genes are transcribed in a temporally regulated manner. the hsv-1 'immediate-early' (ie) promoter sequences contain multiple copies of a hexanucleotide sequence, gggcgg, known as a gc box, and one or more copies of an 11-base pair (bp) conserved a + t-rich element, designated taatgarat. the taatgarat elements are thought to mediate the trans-activation of ie rna synthesis by a virion-associated protein(s), and the flan ... | 1985 | 2993923 |
| demonstration of a stimulatory protein for virus-specified dna polymerase in phorbol ester-treated epstein-barr virus-carrying cells. | a heat-labile epstein-barr virus-specific dna polymerase stimulatory protein having a molecular mass of 45 kda was purified from phorbol 12-myristate 13-acetate-treated p3hr-1 cells by column chromatography. the virus dna polymerase stimulatory protein was precipitated by sera from patients with nasopharyngeal carcinoma but not by sera from healthy donors. the interaction of the stimulatory protein with dna polymerase was stoichiometric. furthermore, this protein stimulated epstein-barr virus dn ... | 1985 | 2994045 |
| nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus dna fragment capable of trans-inducing alpha genes. | the five alpha genes of herpes simplex virus 1 are the first set of genes to be expressed after infection. previous studies have shown that alpha genes resident in eukaryotic cells are induced by infection with herpes simplex virus 1 or 2 but not by other herpesviruses and indicate that the alpha trans-inducing factor was a structural component of the virion. this factor induces genes linked to a bona fide promoter and containing at the 5' end a small sequence derived from the promoter-regulator ... | 1985 | 2994050 |
| glucocorticoid regulation of mouse mammary tumor virus sequences in transgenic mice. | we have introduced a chimeric plasmid, pltr2tk, containing the mouse mammary tumor virus (mtv) long terminal repeat (ltr) linked to the herpes simplex virus type 1 thymidine kinase gene into the mouse germ line by microinjection. in one mouse line, the thymidine kinase gene is appropriately expressed in the lactating mammary glands of heterozygous females; expression also occurs in the ovaries of these mice. in heterozygous males of this line, and in a male derived from another microinjection, t ... | 1985 | 2994051 |
| different populations of herpes simplex virus glycoprotein c discriminated by the carbohydrate-binding characteristics of n-acetylgalactosamine specific lectins (soybean and helix pomatia). brief report. | from the herpes simplex virus specified glycoprotein c two fractions were isolated with affinity either for helix pomatia lectin (hpa) or soybean lectin (sba). the data indicated that hpa and sba, despite their mutual main specificity for n-acetylgalactosamine, recognize structurally different gc populations. | 1985 | 2994599 |
| immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. | serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (hsv1) and 2 (hsv2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (elisa). in the immunoblotting test, polypeptides migrating like the hsv1-specified glycoprotein c showed type-specific reactivity but could not always be detected. the immunoblotting and elisa results were in agreement when antibody responses to hsv1- and hsv2-specified antigens were compared, and they a ... | 1985 | 2995271 |
| hsv1-specific thymidylate kinase activity in infected cells. | several 5-methoxymethyldeoxyuridine (mmdu)-resistant mutants of herpes simplex virus type 1 (hsv1) were classified by measuring their sensitivities to the deoxythymidine kinase (dtk)-dependent antiviral drugs 9-(2-hydroxyethoxymethyl)-guanine (acyclovir, acv), 1-beta-d-arabinofuranosylthymine (arat), and e-(2)-5-bromovinyldeoxyuridine (bvdu) and to the dtk-independent antiviral drug phosphonoacetate (paa). compared to wild-type (wt) virus, all five of the dtk- mutants were highly resistant (grea ... | 1985 | 2995273 |
| asynchronous expression of the immediate-early protein of herpesvirus saimiri in populations of productively infected cells. | the time course of virus replication in cultures of permissive cells infected with high multiplicities of herpesvirus saimiri (hvs), a gammaherpesvirus, is protracted relative to the replication of herpes simplex virus (hsv), an alphaherpesvirus, under similar conditions. the basis for this difference was investigated by quantitative immunofluorescence microscopy exploiting monoclonal antibodies specific to the hvs 52 000 mol. wt. immediate-early polypeptide (ie 52k) and to delayed-early (de 51k ... | 1985 | 2995555 |
| differences in humoral immunogenicity between herpes simplex virus types 1 and 2. | infection of nmri mice with increasing doses of six different strains of herpes simplex virus type 1 (hsv-1) induced increasing levels of neutralizing antibodies. in contrast, three strains of hsv-2, irrespective of the dose, induced only marginal antibody responses. only strain hg 52 (hsv-2) at high doses of infection stimulated antibody formation. the virus content of some organs in 6- to 8-week-old mice appeared to be lower after hsv-2 than after hsv-1 infection. application of immune-modulat ... | 1985 | 2995556 |
| inhibition of herpes simplex virus replication in vitro by human cytotoxic t cell clones and natural killer cell clones. | the abilities of human cytotoxic t cell (ctl) clones and natural killer (nk) cell clones to inhibit the replication of herpes simplex virus (hsv) in vitro were shown. the specificities of clones inhibiting hsv replication were the same as those of cytotoxicity in hsv type specificity and hla restriction, i.e. hsv type 1 (hsv-1) and hsv type 2 (hsv-2) common ctl clones inhibited the replication of both hsv-1 and hsv-2 in autologous cells, but not in allogeneic cells. hsv-1-specific ctl clones inh ... | 1985 | 2995557 |
| a comparison of the acid-soluble polypeptides of five herpesviruses. | the polypeptides soluble in 0.25 m-hcl were extracted from the nuclei of bhk cells infected with herpes simplex virus type 1 or type 2 and separated by sds-page. seventeen polypeptides were detectable in each extract of which 10 type 1 and nine type 2 polypeptides were reproducibly effectively extracted. in cells infected with bovine mammillitis virus, pseudorabies virus or equine herpesvirus type 1, at least 12, 13 and eight polypeptides respectively were acid-soluble. in addition to histones, ... | 1985 | 2995559 |
| acute and latent herpes simplex virus neurological disease in mice immunized with purified virus-specific glycoproteins gb or gd. | groups of 5-week-old balb/c mice were immunized intraperitoneally with approximately 10 micrograms of purified alum-precipitated glycoprotein gb or gd of either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) origin. control mice received injections of alum-precipitated 1% bovine serum albumin (bsa). following a second immunization 4 weeks later, seroconversion was confirmed by demonstrating the presence of glycoprotein-specific antibody by immune precipitation. all animals were challenged ... | 1985 | 2995573 |
| ocular infection with herpes simplex virus (hsv-1) in vitamin a-deficient and control rats. | an experimental model was developed for studying ocular infections with herpes simplex virus (hsv) type 1 in vitamin a-deficient (-a) and pair-fed control (+a) rats. the severity and course of the disease was evaluated by clinical examination, slit lamp biomicroscopy and histopathologic observations. experimental animals were in good health and were infected in the early stages of vitamin deficiency (either prior to or at the beginning of the weight plateau). in all trials the onset of herpetic ... | 1985 | 2995622 |
| (+/-)-2-amino-3,4-dihydro-7-[2,3-dihydroxy-4-(hydroxymethyl)-1- cyclopentyl]-7h-pyrrolo[2,3-d]pyrimidin-4-ones: new carbocyclic analogues of 7-deazaguanosine with antiviral activity. | 5-allyl-2-amino-4,6-dihydroxypyrimidine (3) was chlorinated and ozonized to yield (2-amino-4,6-dichloro-pyrimidin-5-yl)acetaldehyde (5). acetalization of 5 with ethanol afforded a new pyrimidine intermediate 6 which can lead to 2-amino-3,4-dihydro-7-alkyl-7h-pyrrolo[2,3-d]pyrimidin-4-ones and therefore to carbocyclic analogues of 7-deazaguanosine. the 7-substituent was a cyclopentyl analogue of the arabinofuranosyl moiety in 10a, lyxofuranosyl moiety in 10b, and ribofuranosyl moiety in 10c. comp ... | 1985 | 2995667 |
| comparison of the dna sequence and secondary structure of the herpes simplex virus l/s junction and the adeno-associated virus terminal repeat. | the defective parvovirus adeno-associated virus (aav) is absolutely dependent upon coinfection with either adenovirus or herpes simplex virus (hsv) for its multiplication. we have compared the terminal repeats of hsv-1f strain dna with the terminal 200 nucleotides of aav dna. our findings demonstrate similarities between portions of the hsv inverted repeats found at the l/s junction and the termini of aav. by computer analysis we have determined potential secondary folding patterns for both geno ... | 1985 | 2995732 |
| locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences. | computer techniques were used to locate related segments of amino acid sequences in the thymidine kinases of vaccinia virus and of herpes simplex virus type 1 and in porcine adenylate kinase. as determined by a procedure that evaluates triply aligned sequences, the probability that the similarities among the segments described here arose by chance was no greater than 0.001. because the sequence in porcine adenylate kinase is a nucleotide phosphate-binding site it is concluded that the segments i ... | 1985 | 2995987 |
| [genetic transformation of somatic cells. vii. the loss of the transformant phenotype is accompanied by both stable and unstable changes in dna]. | from 6 clones of chinese hamster cells varying in the rate of the loss of transformant phenotype and containing a thymidine kinase gene (tk-gene) of herpes simplex virus type 1 (hsv1), 25 subclones negative in thymidine kinase (tk-) were isolated on a medium with 50 micrograms/ml 5-bromodeoxyuridine (brdu). a study was made of the frequency of spontaneous reversions to the tk+ phenotype in cell populations of brdu-resistant subclones, and of the transforming activity (upon transformation of tk- ... | 1985 | 2996189 |
| [genetic transformation of somatic cells. ix. the loss of the transformant phenotype is accompanied by rearrangements in the plasmid dna containing the thymidine kinase gene of the herpes virus]. | as demonstrated by dot-hybridization, the cells of ht-subclones isolated from the cells of transformant clones cultured on a non-selective medium differ significantly in the number of copies of thymidine kinase gene (tk-gene) of herpes simplex virus (hsv1). since the cells of transformant clones lose thymidine kinase-positive (tk+) phenotype during cultivation, this data are indicative of high frequence rearrangements in the region of transforming dna as responsible for the transformant phenotyp ... | 1985 | 2996190 |
| selection and characterisation of acyclovir-resistant herpes simplex virus type 1 mutants inducing altered dna polymerase activities. | a collection of tk+, acv-resistant mutants of herpes simplex virus type 1 (hsv-1) has been derived using a selection system based on biochemically transformed cells. evidence is presented suggesting that most of these mutants induce resistant dna polymerase activities and are thus likely to express variant dna polymerases. preliminary data on the pathogenesis of these mutants show that most are similar to wild type virus in the majority of their characteristics, although they may be reduced in t ... | 1985 | 2996220 |
| eczema herpeticum of the child. an unusual manifestation of herpes simplex virus infection. | two children aged 7 months with eczema herpeticum received treatment consisting of intravenous acyclovir and human plasma with a high titer of herpes simplex virus antibodies. one recovered following two recurrences, but the other died rapidly, suffering both septicemia due to pseudomonas aeruginosa and herpetic encephalitis. in both cases, the virus involved was a herpes simplex virus type 1 (hsv 1). the various isolates obtained before, during and after treatment remained equally sensitive to ... | 1985 | 2996817 |
| herpes simplex viral infection in human neonates: an immunohistochemical and electron microscopic study. | specimens obtained at autopsy from six neonates with herpes simplex virus (hsv) infections were examined microscopically, electron microscopically, and immunohistochemically. coagulative necrosis with inclusions was found in the livers and adrenal glands in all cases, as well as in various other organs, including the spleen, bone marrow, lungs, esophagus, tongue, and thymus, in some cases. distinct hemorrhagic diathesis was found in three cases. no characteristic clinical findings, such as skin ... | 1985 | 2997017 |
| interferon production and sensitivity of rabbit corneal epithelial and stromal cells. | the induction of interferon and the ability of interferon to induce the antiviral state were studied using rabbit corneal epithelial and stromal cells which were cultured for fewer than five passages. interferon titers in the range of 7000 units/ml were induced in epithelial cell cultures and 76,000 units/ml in stromal cell cultures treated with uv-inactivated bluetongue virus. the interferon induced was stable to ph 2.0 treatment and heating to 56 degrees c for 16 hr. infection of epithelial an ... | 1985 | 2997072 |
| resolution of hsv corneal infection in the absence of delayed-type hypersensitivity. | the role of delayed-type hypersensitivity (dth) in the resolution of herpes simplex virus type 1 (hsv-1) ocular infection was examined. infection of balb/c mice on the sacrificed cornea with hsv-1 resulted in sensitization for dth. this response, demonstrable by swelling of the ear following inoculation with ultraviolet-irradiated virus, was optimal 7 days postinfection. the reaction was immunologically specific and characterized histologically by a predominately mononuclear cell infiltrate. dth ... | 1985 | 2997073 |
| immunological cross-reactivities among three herpesviruses. | sixty adults were tested for humoral and cell-mediated immunity to varicella zoster virus (vzv), type 1 herpes simplex virus (hsv-1) and the human cytomegalovirus (cmv). since herpesviruses share common antigens, we compared results in these individuals to assess whether our tests gave false positives due to cross-reactions. of the 60, igg antibody to vzv, hsv-1, cmv tested by elisa was detected in 51 (85%), 34 (57%) and 20 (33%) respectively. all possible permutations of results were obtained a ... | 1985 | 2997331 |
| effect of cloned human interferons on protein synthesis and morphogenesis of herpes simplex virus. | pretreatment of human fibroblast cells with 100 u of either cloned human alpha-2 or beta interferon per ml for 24 h reduced the release of infectious herpes simplex virus type 1 by more than 99%. this inhibition in infectivity correlated well with the total number of extracellular virus particles released from treated cells as determined by dna dot blot hybridization analysis. electron microscopic observations of interferon-treated human fibroblast cells clearly demonstrated typical assembly of ... | 1985 | 2997465 |
| humoral immune response to herpes simplex virus type 2 glycoproteins in patients receiving a glycoprotein subunit vaccine. | serial serum specimens from 22 herpes simplex virus (hsv)-seronegative recipients of an hsv type 2 (hsv-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to hsv-2 gb, gd, and g80, a complex of gc and ge. volunteers received 50 (n = 12) or 100 micrograms (n = 10) of vaccine at days 0, 28, and 140; sera were drawn weekly for 8 weeks and again at days 140, 147, and 365. among seronegative volunteers, an ... | 1985 | 2997470 |
| specificity of fc receptors induced by herpes simplex virus type 1: comparison of immunoglobulin g from different animal species. | cells infected with herpes simplex virus type 1 (hsv-1) express a cell surface receptor able to bind the fc portion of immunoglobulin g (igg). of the four human igg subclasses, the hsv-1 fc receptor, like staphylococcal protein a, binds to all except igg3. in this paper, we describe the binding of a number of animal igg and igg subclass molecules to hsv-1-infected cells and compare this binding to that of protein a. although only few representatives from each animal order were tested, we found t ... | 1985 | 2997471 |
| utilization of internal aug codons for initiation of protein synthesis directed by mrnas from normal and mutant genes encoding herpes simplex virus-specified thymidine kinase. | previous studies (h.s. marsden, l. haarr, and c.m. preston, j. virol. 46:434-445, 1983) have shown that at least three polypeptides, with molecular weights of 43,000, 39,000, and 38,000, are encoded by the herpes simplex virus type 1 (hsv-1) thymidine kinase (tk) gene. it has been suggested that the 39,000- and 38,000-molecular-weight polypeptides arise from preinitiation complexes bypassing the first and second aug codons before commencement of translation since, according to previous work (m. ... | 1985 | 2997472 |
| isolation and characterization of deletion mutants of herpes simplex virus type 1 in the gene encoding immediate-early regulatory protein icp4. | using vero cells transformed with the wild-type gene for icp4 as the permissive host cell, we isolated herpes simplex virus type 1 (hsv-1) mutants containing deletions in both copies of the icp4 gene. the mutants, d120 and d202, contained deletions of 4.1 and 0.5 kilobases, respectively, in each copy of icp4. icp4 mrna synthesized in d202-infected vero cells was 0.5 kilobases smaller than that synthesized in cells infected with the wild-type virus. no icp4 mrna was detected in d120-infected vero ... | 1985 | 2997476 |
| [occurrence of antibodies against herpes simplex virus type 1 in patients treated by long-term dialysis and in personnel of the dialysis department]. | 1985 | 2997759 | |
| stable induction of a 51k cellular protein in neuronal cells surviving herpes simplex virus type 1 infection. | a series of survivor cell lines derived by infection of b103 rat neuroma cells with active wild-type herpes simplex virus type 1 (hsv-1) (m. levine, a. l. goldin, and j. c. glorioso, j. virol. 35, 203-210 (1980)) has been isolated. the survivor cells produced no infectious virus, yet they continued to react with hsv-1 antiserum for over 100 cell generations following the initial infection. the reactivity of the survivor cells with hsv-1 antiserum is characterized as being due to expression of a ... | 1985 | 2997992 |
| activation and inhibition of expression of the 72,000-da early protein of adenovirus type 5 in mouse cells constitutively expressing an immediate early protein of herpes simplex virus type 1. | it has been previously reported that immediate early proteins of pseudorabies and cytomegalo viruses can substitute for the products of the human adenovirus type 5 (ad5) e1a gene in the activation of early ad5 transcription. in the present report the effect of one of the herpes simplex virus type 1 (hsv-1) immediate early genes, icp4, on ad5 early gene expression has been examined using mouse cell lines that constitutively express icp4. these lines as well as nonproducers were infected with wild ... | 1985 | 2998046 |
| effect of herpes simplex virus type 1 on cellular pools of oligosaccharide-lipid. | incorporation of [3h]mannose into cellular pools of mannosylphosphoryl dolichol (man-p-dol), oligosaccharide-lipid, and glycoprotein was measured and compared in herpes simplex virus type 1 (hsv-1)-infected cells and -uninfected cells. while mannose incorporation into the monosaccharide-dolichol fraction was similar in infected or uninfected vero cells, incorporation into the oligosaccharide-lipid fraction was markedly reduced in hsv-1-infected cells (64% of control levels). in contrast, mannose ... | 1985 | 2998058 |
| the antiherpes drug (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) interferes with formation of n-linked and of o-linked oligosaccharides of the herpes simplex virus type 1 glycoprotein c. | in hsv-1 infected cell the antiherpes drug (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) exerted at least three different effects on glycosylation of glycoprotein gc. first, an overall decrease of protein glycosylation occurred due to inhibition of synthesis of the lipid-linked oligosaccharides, precursors of n-linked oligosaccharides of gc. second, an inhibition of processing of n-linked oligosaccharides occurred after the acquisition of endo h-resistance, and possibly due to inhibition of galact ... | 1985 | 2998063 |
| loss of surface fibronectin after infection of cultured cells by hsv-1 and 2. | fibronectin is lost from the surface of hsv infected cells during cell rounding. in order to investigate also the fate of fibronectin during the process of hsv-induced cell-fusion, bhk, vero as well as primary or secondary rabbit kidney cells were infected with hsv-1 strains producing cell-fusion. by immunofluorescence and immunoelectron microscopy a considerable loss of fibronectin after hsv infection could be demonstrated leaving only irregular clumps of fibronectin containing virus particles ... | 1985 | 2998301 |
| [synthesis of anomeric 5-trimethylgermyl-2'-deoxyuridines and study of their antiviral and cytotoxic properties]. | glycosylation of silylated 5-trimethylgermyluracil with 2-deoxy-3,5-di-o-p-toluyl-alpha-d-ribofuranosylchloride in dichloroethane in the presence of sncl4 and subsequent deacylation led to anomeric 5-trimethylgermyl-2'-deoxyuridines. the alpha-nucleoside inhibits hsv-1 replication in vitro, blocks 2'-deoxyuridine incorporation into dna of hepatoma 22a cells and incorporation of thymidine into dna of cancer ovarian cells as well. treatment with 125 mg/kg x 5 days of alpha-nucleoside fails to incr ... | 1985 | 2998405 |
| activation of cellular promoters during herpes virus infection of biochemically transformed cells. | at least two of the immediate-early (ie) products of herpes simplex virus-1 (hsv-1) are responsible for the activation of transcription from viral early promoters. this process appears not to be promoter specific since several unrelated viral and cellular plasmid-borne promoters can also be activated in short-term transfection assays. this paper describes experiments that show that cellular promoters integrated into the host genome can also be activated during viral infection, and that this proc ... | 1985 | 2998778 |
| intermolecular recombination of the herpes simplex virus type 1 genome analysed using two strains differing in restriction enzyme cleavage sites. | intermolecular recombination of herpes simplex virus type 1 (hsv-1) was studied by analysing the segregation of strain-specific restriction enzyme cleavage sites among progeny viruses produced after co-infection by two hsv-1 strains differing in eight restriction enzyme cleavage sites. out of 93 progeny viruses examined, 51 clones were recombinant, and crossover sites of the recombinants were mapped on the hsv-1 genome. these sites were distributed evenly in the unique sequence of the l componen ... | 1985 | 2999310 |
| inhibition of herpes simplex virus type 1-induced interferon synthesis by monoclonal antibodies against viral glycoprotein d and by lysosomotropic drugs. | components of herpes simplex virus remained bound to the diploid cell membrane after nucleocapsid penetration into the cytosol. these components enabled the infected cells to induce interferon-alpha (ifn-alpha) in peripheral blood mononuclear cells even when the infected cells were fixed by glutaraldehyde. monoclonal antibodies directed against the major viral glycoprotein d could neutralize their ifn-alpha-inducing capacity. thus, the process of ifn induction does not require uptake and penetra ... | 1985 | 2999320 |
| synthetic glycoprotein d-related peptides protect mice against herpes simplex virus challenge. | glycoprotein d (gd) of herpes simplex virus (hsv) protects mice from a lethal challenge by either hsv type 1 (hsv-1; oral) or hsv-2 (genital). we evaluated whether synthetic peptides representing residues 1 through 23 of gd (mature protein) can be used as a potential synthetic herpesvirus vaccine. the immunogenicity of the peptides was demonstrated by the biological reactivity of antipeptide sera in immunoprecipitation and neutralization assays. all sera which immunoprecipitated gd had neutraliz ... | 1985 | 2999419 |
| effect of herpes simplex virus types 1 and 2 on surface expression of class i major histocompatibility complex antigens on infected cells. | cytotoxic t lymphocytes (ctl) generated in c57bl/6 (h-2b) mice in response to infection with the serologically distinct herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) were cross-reactive against target cells infected with either serotype. however, hsv-2-infected cells were shown to be much less susceptible to ctl-mediated lysis, and analysis through the use of hsv-1 x hsv-2 intertypic recombinants mapped the reduced susceptibility to a region contained within 0.82 to 1.00 map units of the ... | 1985 | 2999432 |
| epstein-barr virus genome may encode a protein showing significant amino acid and predicted secondary structure homology with glycoprotein b of herpes simplex virus 1. | we report significant sequence and predicted secondary structure homology between the herpes simplex virus 1 glycoprotein b (gb) and a protein predicted to be encoded by the balf4 reading frame of epstein-barr virus (ebv). homology was detectable at the dna level and was highly significant at the protein level and when evolutionary substitution frequencies of amino acids in related proteins were taken into account. hydropathic analyses predicted that the two proteins possess conserved n-terminal ... | 1985 | 2999435 |
| a modular system for the assay of transcription regulatory signals: the sequence taatgarat is required for herpes simplex virus immediate early gene activation. | a modular system for assaying the activity of transcriptional regulatory signals based on herpes simplex virus (hsv) promoter and terminator sequences linked to the bacterial chloramphenicol acetyltransferase (cat) gene has been used to study activation of hsv immediate early (ie) gene expression. insertion of the sv40 72 base pair (bp) repeat increased mrna levels by 15-fold thus demonstrating the ability of the hsv ie promoter to respond to a heterologous enhancer. a fragment containing part o ... | 1985 | 2999706 |
| dna sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters. | previous work has shown that transcriptional activation of herpes simplex virus type 1 (hsv-1) immediate early genes is mediated by a protein species (vmw65) present in the tegument of infecting virions. this paper describes dna sequence analysis and mrna mapping of the vmw65 gene in hsv-1 strain 17. the vmw65 coding region was identified as a 490 codon sequence encoding a polypeptide of molecular weight 54,342 and characterised by a high proportion of charged amino acid residues. a homologue to ... | 1985 | 2999707 |
| dna sequence of the region in the genome of herpes simplex virus type 1 containing the genes for dna polymerase and the major dna binding protein. | in the long unique region of the genome of herpes simplex virus type 1 (hsv-1), the genes for dna polymerase and the major dna binding protein are arranged in a head to head manner, with an origin of dna replication (termed oril) located between them. this paper reports an 8400 base pair dna sequence containing both genes and the origin, obtained mostly by m13/dideoxy analysis of plasmid cloned fragments. amino acid sequences of the two proteins were deduced. homologues of both genes were detect ... | 1985 | 2999714 |
| [discovery of the sequences of herpes simplex virus type 1 in the genomes of normal and transformed mouse and hamster cell lines]. | sequences capable of hybridization with cloned fragments of herpes simplex virus type i (hsvi) dna were found in genome dna of syrian hamster fibroblast lines transformed either by intact hsvi genome (cell line 14.012.81) or this virus dna fragments (cell line eh/a44). the method of pinpoint hybridization demonstrated the presence in dna of the cell lines under study of sequences capable of hybridization both with unique areas and with hsvi genome replicas. a correlation was established between ... | 1985 | 3000081 |
| possible relationship between antigenic properties and isolation history of hsv-1 strains. | 29 monoclonal anti-herpes simplex virus (hsv)-1 antibodies were produced and characterized with regard to virus neutralizing activity, intracellular or cell-surface location of viral antigens and, where possible, molecular weight of the viral protein recognized. 13 antibodies recognized viral antigens expressed on the surface of infected cells and 16 were directed to intracellular viral components. only two antibodies exhibited virus neutralizing activity. application of these antibodies to an a ... | 1985 | 3000104 |
| recovery of herpesviruses from cerebrospinal fluid of immunodeficient homosexual men. | over a one-year period the cerebrospinal fluid (csf) obtained from a series of homosexual men immunocompromised with either hodgkin's disease or acquired immune deficiency syndrome (aids) was cultured to assess the frequency with which infectious viruses could be recovered. of 58 patients examined, 4 (6.9%) had csf cultures that showed a cytopathology consistent with a virus infection. all isolates proved to be herpesviruses. cytomegalovirus (cmv) and varicella-zoster virus were isolated from cs ... | 1985 | 3000285 |
| antiviral activity of 5-ethyl-2'-deoxyuridine against herpes simplex viruses in cell culture, mice, and guinea pigs. | the susceptibility of 3 laboratory strains and 24 clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 5-ethyl-2'-deoxyuridine was determined in plaque reduction assays in vero cells. the median effective doses were 8.6 and 7.8 microm, respectively. the drug was less potent than acyclovir and other related antiviral drugs, but it had a high therapeutic index against both hsv-1 and hsv-2. drug-resistant viruses were readily produced in cell culture. these variants were c ... | 1985 | 3000291 |
| fusion from without induced by herpes simplex virus type 1. | strains ang and ang path of herpes simplex virus type 1 (hsv1) produced fusion from without (ffwo) of cells in culture. ffwo required 45 min to become complete. in contrast, fusion from within (ffwi) was not detected until 3-4 h after infection, depending on the cell type. ffwo was temperature dependent: at 0 degrees no fusion could be observed, but increase of temperature increased the degree of fusion. the ph optimum for ffwo was 7.8-8.5. the ffwo activity of the virus was found to be slightly ... | 1985 | 3000981 |
| recurrent genital herpes simplex virus infection in guinea pigs. | after recovery from initial genital herpes simplex virus (hsv) infections, female guinea pigs developed spontaneous recurrent infections characterized by discrete erythematous or vesicular herpetic lesions on the external genital skin. hsv type 2 (hsv2) caused significantly more recurrent infections in guinea pigs than did hsv type 1 (hsv1). hsv2-infected animals demonstrated a significant decline in frequency of recurrences over time. the viral nature of the recurrent lesions was confirmed by r ... | 1985 | 3000982 |
| establishment of mutant fm3a murine mammary carcinoma cell strains transformed with the herpes simplex virus type 1 thymidine kinase gene. | to establish cell systems appropriate for investigating the mode of action of anti-herpetic nucleoside analogs, mutants were constructed from murine fm3a mammary carcinoma cells, which were deficient in both thymidine kinase (ec 2.7.1.21) and thymidylate synthase (ec 2.1.1.45), but were transformed with a recombinant plasmid dna containing the herpes simplex virus type 1 thymidine kinase gene. the transformed cells expressed viral thymidine kinase activity and showed unusually high sensitivity t ... | 1985 | 3001011 |
| typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid spot hybridization. | fifty-one clinical isolates of herpes simplex virus (hsv) were typed by an enzyme immunoassay (eia) using mouse monoclonal antibodies, by dna spot hybridization, and by restriction enzyme analysis using restriction endonuclease eco ri. extracts of vero cells infected with the isolates were used for coating microtitre plates or denatured and spotted onto nitrocellulose filters. viral antigens passively adsorbed to microtitre plates were detected by an indirect eia using mouse monoclonal antibodie ... | 1985 | 3001118 |
| detection of herpes simplex virus type 2-specific antibody with glycoprotein g. | a recently described herpes simplex virus (hsv) type 2 (hsv-2)-specific glycoprotein (gg-2) was purified on an immunoaffinity column prepared with monoclonal antibody. this purified antigen was used in an immunodot enzymatic assay on nitrocellulose paper for the detection of hsv-2 antibodies in human serum. the test was very sensitive in that hsv-2 antibodies were detected in the convalescent sera of 132 of 134 patients with recurrent genital infections in which hsv-2 had been isolated earlier. ... | 1985 | 3001136 |
| herpes simplex virus hepatitis in mice: effects of treatment with trisodium phosphonoformate. | clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) induced focal necrotic hepatitis in icr/schö mice. the extent of the hepatitis could be considerably reduced by trisodium phosphonoformate (pfa) administered for 3 days in a daily dose of 400-600 mg/kg. the hsv hepatitis in mice could serve as a suitable model for testing antiherpetic drugs. | 1985 | 2869661 |
| alteration of tyrosine hydroxylase activity in pc12 cells infected with herpes simplex virus type 1. | during infection with herpes simplex virus type 1 (hsv-1) the activity of tyrosine hydroxylase (th) in pc12 pheochromocytoma cells was initially depressed reaching a nadir at 6 hours post-inoculation, but recovered rapidly with a return to baseline activity by 8 to 9 hours post-inoculation. subsequently, th activity again fell with a second more variable rise in activity occurring at 24 hours post-inoculation. studies with metabolic inhibitors and 2 temperature-sensitive viral mutants indicated ... | 1985 | 2857560 |
| liquid-phase and solid-phase radioimmunoassay with herpes simlex virus type 1 nucleocapsids. | liquid-phase radioimmunoassay (lpria) and solid-phase radioimmunoassay (spria) are described utilizing either 125i-labelled or immobilized nucleocapsids (nc) of herpes simplex virus type 1 (hsv-1). these techniques appeared sensitive and specific for quantification of hsv-nc antigens and corresponding antibodies. | 1985 | 2860794 |
| synthesis of glycoproteins of syncytial and non-syncytial strains of herpes simplex virus type 1 and their transport to the plasma membrane. | underglycosylated form of glycoprotein c (gc) synthesized in the presence of tunicamycin was found on the surface membrane of bhk cells infected with the non-syncytial (non-syn) strain kupka of herpes simplex virus type 1 (hsv-1). such form of gc was not detected in the plasma membrane of tunicamycin-treated cells infected with the syncytial (syn) hszp strain of hsv-1. in comparison to the non-syn strain kupka, a smaller amount of glycoproteins of the syn hszp strain was detected on the surface ... | 1985 | 2864818 |
| anti-herpes simplex virus and cytostatic activity of some new 5-substituted 1-(4-hydroxybutyl)-and 1-(2-hydroxyethoxymethyl) uracil nucleoside analogues. | two series of 5-substituted acyclic uracil nucleoside analogues (5-x-acyclo-u) were evaluated for their inhibitory effects against three herpes simplex virus type 1 (hsv-1) strains and one type 2 (hsv-2) strain in a plaque inhibition assay on human embryonic lung fibroblast (helf) cell cultures as well as for their ability to inhibit the proliferation of baby hamster kidney cells in suspension (bhk-s) culture. acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] and (s)-9-(2,3-dihydroxypropyl)adeni ... | 1985 | 2864819 |
| host cell and virus strain differences in synthesis of immediate early polypeptides in hsv-1 infected cells. | the synthesis of the immediate early (ie) polypeptides was analysed in primary rabbit kidney (rk) cells and a stable line of rabbit lung (zp) cells infected with the syncytial (syn) strain hszp and the non-syn strain kos of herpes simplex virus type 1 (hsv-1). results showed the following: after cycloheximide reversal the infection of rk and zp cells with hszp strain led to synthesis of five ie polypeptides (175k, 136k, 87k, 68k, and 63k), while infection of both cell cultures with the kos strai ... | 1985 | 2866689 |
| comparative studies on activation of latent herpes simplex virus in rabbits. | latent infection was established in 113 rabbits by inoculation of herpes simplex virus type 1 (hsv-1) strain kupka into the right scarified cornea. in animals succumbed from 88-387 days post-infection (p.i.), hsv-1 was detected in the cultured right cornea (rc) fragments of 2 out 45 rabbits (4.4%), while the cultured right trigeminal ganglion (rtg) fragments yielded virus in 60 out 70 animals (85.7%). spontaneous virus shedding occurred nearly in the half (35) of 74 animals followed for a mean o ... | 1985 | 2866691 |
| membrane markers, target cell specificity, and sensitivity to biological response modifiers distinguish human natural cytotoxic from human natural killer cells. | in the present report, we provide evidence for the distinct existence of a human natural cytotoxic (hnc) cell. this hnc cell can be identified by the monoclonal antibody hnc-1a3 and by the absence of the t10 antigen, other antigenic markers being shared, at least in part, with natural killer (nk) cells, t cells, or monocytes. in addition, the hnc cell preferentially kills the ma-160 target, the herpes simplex virus-1-infected ma-160 cell line, and the two human tumor cell lines hep-2 and hf-2. i ... | 1985 | 2932473 |
| investigation of the virulence genes of herpes simplex virus 2 by experimental infection in vivo with defined intertypic recombinants of a virulent hsv-2 x an avirulent hsv-1. | for determination of the gene loci for virulence and tropism of herpes simplex virus 2 (hsv-2) intertypic recombinants of hsv-1 strain hfem and hsv-2 strain 3345 were screened for their pathogenicity in the tree shrew. hsv-1 strain hfem is not pathogenic in the tree shrew and can be recovered as a latent virus only from the spleen of the animals; but hsv-2 strain 3345, the other parental virus of the recombinants, was found to be virulent for the tree shrew and colonized the ganglia of latently ... | 1985 | 3003551 |
| [use of permeabilized cells in the study of inhibitory products of herpes virus replication]. | cells made permeable by exposure to lysolecithin following infection by hsv-1 synthesize dna (in greater amounts than non-infected cells) in the presence of the four deoxyribonucleoside-triphosphates (dntps) : datp, dctp, dgtp, and dttp. dna synthesis also occurs if dttp is replaced by dt or dtmp, indicating activity of enzymes such as thymidine kinase, thymidylate kinase, deoxyribonucleoside-diphosphate kinase and adn polymerase. examination of dna synthesis in permeabilized cells enables detec ... | 1985 | 3003658 |
| selective inhibition of the proliferation of herpes simplex virus type 1 thymidine kinase gene-transformed murine mammary fm3a carcinoma cells by (e)-5-(2-bromovinyl)-2'-deoxyuridine and related compounds. | mouse mammary carcinoma fm3a cells deficient in thymidine kinase were transformed by a cloned gene for herpes simplex virus type 1 thymidine kinase. among several anti-herpetic nucleoside analogues, (e)-5-(2-bromovinyl)-2'-deoxyuridine, (e)-5-(2-iodovinyl)-2'-deoxyuridine and (e)-5-(2-bromovinyl)-2'-deoxycytidine inhibited the growth of the transformed cells at concentrations 5000- to 20000-fold lower than those required to inhibit the growth of the corresponding wild-type cells. the selective i ... | 1985 | 3003708 |
| highly selective cytostatic activity of (e)-5-(2-bromovinyl)-2'-deoxyuridine derivatives for murine mammary carcinoma (fm3a) cells transformed with the herpes simplex virus type 1 thymidine kinase gene. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and various structurally related analogues thereof, i.e., (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) and (e)-5-(2-bromovinyl)-2'-deoxycytidine (bvdc), and the carbocyclic analogues of bvdu, ivdu, and bvdc, were evaluated for their inhibitory effects on the growth of murine mammary carcinoma fm3a cells, deficient in thymidine kinase (tk) activity but transformed with the herpes simplex virus type 1 (hsv-1) tk gene (designated fm3a/tk-/hsv-1 tk+). bvdu a ... | 1985 | 3001499 |
| risk of recurrence after treatment of first-episode genital herpes with intravenous acyclovir. | to determine whether intravenous acyclovir treatment for a first episode of genital herpes could prevent or reduce subsequent recurrences, we combined and analyzed the results of two independently conducted, randomized, double-blind, placebo-controlled studies. sixty-one patients were enrolled in the two trials; 30 received the drug, and 31 received placebo. at entry the demographic, epidemiologic, and clinical features of acyclovir- and placebo-treated patients from the two centers showed no si ... | 1985 | 3001952 |
| characterization of virus obtained from mdbk cells persistently infected with a variant of herpes simplex virus type 1 strain mp [hsv-1(mp)]. | virus clones which express glycoprotein gc (gc+) were obtained from two persistently infected (p.i.) mdbk cell lines which had been independently established by infection with hsv-1(mp)10311, a gc- syncytial (syn) variant of herpes simplex virus type 1 strain mp [hsv-1(mp)]. the gc+ revertants were syn in mdbk, hep-2, and vero cell lines and in primary human fibroblasts; this offers further evidence that glycoprotein gc does not inhibit cell fusion. the gc+ revertants represented from 70 to 100 ... | 1985 | 3002019 |
| expression of herpes simplex virus glycoprotein b gene in yeast. | the dna sequence coding for herpes simplex virus type 1 glycoprotein b was placed under control of the acid phosphatase promoter of the yeast saccharomyces cerevisiae in a plasmid capable of replicating in both yeast and escherichia coli. yeast transformed by the plasmid synthesized immunologically active glycoprotein b polypeptide. | 1985 | 3002066 |
| herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method. | autopsy specimens from six patients with clinically diagnosed herpes simplex virus (hsv) encephalitis were studied. since immunocytochemistry has been reported to be a more reliable and successful method to identify hsv as the etiologic agent, antitype 1 hsv (hsv1) and antitype 2 hsv (hsv2) and the avidin-biotin-peroxidase complex (abc) method were applied to brain paraffin sections. positive immunostaining was observed in front-orbital, mediobasal temporal lobes, cingulate gyrus, and insula. th ... | 1985 | 3002087 |
| drug resistance patterns of herpes simplex virus isolates from patients treated with acyclovir. | a decrease in the in vitro sensitivity to acyclovir (acv) was observed in successive isolates of herpes simplex virus type 1 from three immunocompromised patients during intravenous therapy with this drug. the acv-resistant isolate from patient 1 was cross-resistant to dihydroxypropoxymethylguanine and bromovinyldeoxyuridine, but still susceptible to three fluoro-substituted pyrimidines, 2'-fluoro-5-iodo-1-beta-d-arabinofuranosylcytosine (fiac), 2'-fluoro-5-iodo-1-beta-d-arabinofuranosyluracil ( ... | 1985 | 3002245 |
| synergistic interaction between interferon-alpha and acyclovir in the treatment of herpes simplex virus type 1 infection in mice. | hairless mice were infected intracutaneously with hsv-1 and treated with rhuifn-alpha a/d, a recombinant dna-derived hybrid human interferon-alpha that is active on mouse cells in vitro and in vivo. when given alone (1 or 2 x 10(5) units/dose) at times soon after infection, interferon showed some efficacy, reducing disease severity by 20-30% compared to control. oral acyclovir was also effective in reducing disease severity in a dose-dependent manner, even when treatment was begun 72 h post-infe ... | 1985 | 3002258 |
| herpes simplex virus vaccines. | 1985 | 3002260 | |
| incorporation of carbocyclic (e)-5-(2-iodovinyl)-2'-deoxyuridine (c-ivdu) into dna of herpes simplex virus-infected cells. | c-ivdu, the carbocyclic analogue of ivdu [(e)-5-(2-iodovinyl)-2'-deoxyuridine], in which the sugar moiety is replaced by a cyclopentane ring, is, like its parent compound, a very potent and selective inhibitor of herpes simplex virus type 1 (hsv-1) replication. both c-ivdu and ivdu are effectively phosphorylated by hsv-1 infected cells and incorporated into viral and cellular dna of these cells. | 1985 | 3002266 |
| [inhibition of virus multiplication by immunoactive peptides]. | in the present study we show that peritoneal macrophages obtained from the mice treated with the immunoactive peptides inhibit the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), but not that of vesicular stomatitis virus (vsv), and that the intraperitoneal administration of the peptides suppresses the infection with hsv-1 in mice. | 1985 | 3002570 |
| acyclovir prophylaxis in bone marrow transplant recipients. | forty-two patients undergoing bone marrow transplantation were included in a randomised, double-blind and placebo controlled trial of prolonged acyclovir prophylaxis against infections with viruses of the herpes group. twenty patients were allocated to receive acyclovir and 22 to receive placebo. acyclovir or placebo was administered i.v. at a dose of 250 mg/m2 twice daily, starting 5 days before transplantation. at 5 weeks after transplantation, administration was changed to tablets, 400 mg thr ... | 1985 | 3006228 |
| herpesvirus infection in man. | herpesviruses which affect man are herpes simplex virus type 1 and type 2, varicella-zoster virus, cytomegalovirus and epstein-barr virus. the review deals with the more common clinical manifestations of human herpesvirus infections, which occur ubiquitously in all populations throughout the world. primary infections most commonly occur in childhood. it is a characteristic feature of herpesvirus that they generally remain in a latent form after clearance of the primary infection. the overall maj ... | 1985 | 3006233 |
| virological aspects on herpesvirus infections. | the family herpesviridae include five important human pathogens, herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv) and cytomegalovirus (cmv). the five viruses are represented in all three herpesvirus subfamilies, a subdivision based on differences in phenotypic properties such as length of replication cycle, cytotoxic properties and host cell range. the herpesviruses have dna genomes sharing characteristic anatomical features. they replicate in the nuclei of eukaryo ... | 1985 | 3006235 |
| herpetic tracheobronchitis detected at bronchoscopy: cytologic diagnosis by the immunoperoxidase method. | herpes simplex virus (hsv) infection of the respiratory tract requires rapid specific diagnosis to avoid late complications and maximize the efficacy of available drug therapy. we report a method of accomplishing this that was tested in 33 cytologic specimens derived from sputum, washings, or brushings from 25 debilitated elderly males suffering from a variety of underlying neoplastic and nonneoplastic chronic diseases. all specimens had shown either single cells (54%), multinucleated groups (8% ... | 1985 | 3013535 |
| the antiviral activity of tetrazole phosphonic acids and their analogues. | 5-(phosphonomethyl)-1h-tetrazole and a number of related tetrazoles have been prepared and their effects on the replication of herpes simplex viruses-1 and -2 have been investigated as well as their abilities to inhibit the dna polymerases induced by these viruses and the rna transcriptase activity of influenza virus a. contrary to an earlier report, 5-(phosphonomethyl)-1h-tetrazole was not an efficient inhibitor of the replication of hsv-1 and hsv-2 in tissue culture. analogues of 5-(phosphonom ... | 1985 | 2417198 |
| evaluation of corynebacterium granulosum derived p40 fraction effects on macrophage anti-herpes simplex virus type 1 functions. | 1985 | 2417550 | |
| the t cell response to the glycoprotein d of the herpes simplex virus: the significance of antigen conformation. | synthetic peptides corresponding to the first 23 amino acids of the glycoprotein d molecule of herpes simplex virus have been used to immunize mice and examine the role of antigen conformation in t cell responses. the structure of the peptides was determined by circular dichroism studies and was shown to be consistent with theoretical structure predictions. t cell clones were found that could respond to peptides that were nonhelical, and this response, which was directed to the c-terminal determ ... | 1985 | 2409148 |
| the immune response to herpes simplex virus: comparison of the specificity and relative titers of serum antibodies directed against viral polypeptides following primary herpes simplex virus type 1 infections. | employing an immunoblotting technique, the polypeptide specificity and relative titers of anti-hsv igg reactive with denaturation-resistant epitopes on hsv proteins were determined in patients experiencing primary hsv-1 infections at various anatomical sites. early sera from previously seronegative patients with primary hsv-1 infections were found to have comparatively low levels of antibody directed against the major viral glycoprotein antigens (gb, gc, and gd) relative to titers present in ser ... | 1985 | 2409225 |
| establishment and characterization of a persistent infection of mdbk cells with herpes simplex virus. | a persistent, dynamic-state infection of a variant of herpes simplex virus type 1 strain mp [hsv-1 (mp)] in mdbk cells was established without supportive measures and maintained for over three years by routine passaging of the cells at 7-9 day intervals. the infection was characterized by a cyclic pattern of monolayer damage and reconstitution, correlated with virus production, which was most evident when the interval between subcultures was intentionally prolonged and the cells were left undist ... | 1985 | 2409431 |
| the ribonucleotide reductase induced by herpes simplex virus type 1 involves minimally a complex of two polypeptides (136k and 38k). | herpes simplex virus type 1 (hsv-1) encodes a polypeptide of apparent mol. wt. 136 000 (vmw136) known to be a component of the virus-specified ribonucleotide reductase. monoclonal antibodies that precipitate this polypeptide also precipitate a polypeptide of mol. wt. 38 000 (vmw38) from extracts of hsv-1-infected cells. the basis for this co-precipitation has been investigated using a monoclonal antibody directed against vmw136 and an oligopeptide-induced antiserum directed against the carboxy t ... | 1985 | 2410556 |
| prediction of sequential antigenic regions in proteins. | prediction of antigenic regions in a protein will be helpful for a rational approach to the synthesis of peptides which may elicit antibodies reactive with the intact protein. earlier methods are based on the assumption that antigenic regions are primarily hydrophilic regions at the surface of the protein molecule. the method presented here is based on the amino acid composition of known antigenic regions in 20 proteins which is compared with that of 314 proteins [(1978) atlas of protein sequenc ... | 1985 | 2411595 |