Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| colchicine-like effect of diethylstilbestrol (des) on mammalian cells in vitro. | diethylstilbestrol (des), a synthetic estrogen, showed colchicine-like effects in vitro on cells of the cell lines such as chinese hamster fibroblast of thymus origin (cht), rat liver (dl), rat erythroblastic leukemia (eden-1/tc) and hela-s3. metaphase arrest was induced 3 h after treatment with 15 microgram/ml of des and polyploid or polynucleated cells were prominently observed more than 24 h after treatment. the arrest, however, was reversible when the agent was removed from the medium. tetra ... | 1978 | 661838 |
| repair of alkylated dna in chinese hamster cells measured by loss of enzyme-sensitive sites in isolated dna. | 1978 | 666813 | |
| altered growth properties of chinese hamster cells exposed to 1-methylguanine and 7-methylguanine. | primary chinese hamster embryo cell cultures generally yield cell lines with a finite lifetime in culture. however, if early-passage cells are exposed chronically to either of two normal degradation products of transfer rna, 1-methylguanine or 7-methylguanine, they are converted to continuous lines with altered growth characteristics and morphology. the continuous cell lines have saturation densities 2- to 10-fold higher than did finite control cultures, and some have the ability to grow in soft ... | 1978 | 667823 |
| assignment of nadh-cytochrome b5 reductase (dia1 locus) to human chromosome 22. | nadh-cytochrome b5 reductase (dia1, ec. 1.6.2.2) from human fibroblasts and from chinese hamster cells, both identified by immunologic studies, were clearly distinguished after polyacrylamide gel isoelectro-focusing followed by staining for nadh diaphorase activity. in thirteen independent man-hamster hybrids, the human enzyme dia1 presented a positive correlation with the human chromosome g22. eight hybrids were dia1(+) g22(+) and five hybrids were dia1(-) g22(-). these data agree with the rece ... | 1978 | 669708 |
| dna single-strand lesions due to 'sunlight' and uv light: a comparison of their induction in chinese hamster and human cells, and their fate in chinese hamster cells. | 1978 | 674395 | |
| structure of the altered oligosaccharide present in glycoproteins from a clone of chinese hamster ovary cells deficient in n-acetylglucosaminyltransferase activity. | clone 15b cells, derived from chinese hamster ovary cells and deficient in a specific udp-n-acetylglucosamine:glycoprotein n-acetylglucosaminyltransferase activity, synthesize glycoproteins with altered oligosaccharide units. glycopeptides prepared from these glycoproteins contain large quantities of a glycopeptide with the composition (man)5(glcnac)2-asn whereas parent cells have only small amounts of this glycopeptide. the structure of the glycopeptide was determined by the combination of meth ... | 1978 | 681360 |
| induced segregation of human syntenic genes by 5-bromodeozyuridine + near-visible light. | chromosome-breaking agents have been used in two different procedures for promoting segregation of syntenic genes on human chromosome 12. in method a, a human-chinese hamster cell hybrid containing the single human chromosome 12 was treated either with 5-bromodeoxyuridine brdu + near-visible light or with x-rays. in method b, normal human fibroblasts were treated with brdu + near-visible light followed by their fusion with a chinese hamster glycine-requiring cell mutant cho-k1/gly-a. since the h ... | 1978 | 684558 |
| ghost-mediated transfer of human hypoxanthine-guanine phosphoribosyl transferase into deficient chinese hamster ovary cells by means of polyethylene glycol-induced fusion. | 1978 | 689099 | |
| assignment of peptidase s (peps) to chromosome 4 in man using somatic cell hybrids. | a starch gel electrophoretic procedure is described that resolves peptidase s (peps) as well as the peptidases a, b, and c in man-rodent, rodent-rodent, and primate-rodent interspecific somatic cell hybrids. the interspecific peps cell hybrid phenotype can be resolved into a pattern which suggests that peps is composed of five or six identical subunits. results are presented supporting assignment of the peps locus to chromosome 4 in man using man-mouse and man-chinese hamster somatic cell hybrid ... | 1978 | 689684 |
| hypoxanthine phosphoribosyltransferase from chinese hamster brain and human erythrocytes. | 1978 | 692400 | |
| genetic analysis of tumorigenesis: i. expression of tumor-forming ability in hamster hybrid cell lines. | two new fibroblasts cell lines from a male chinese hamster embryo are described: one tumorigenic in nude mice (chef 16-2) and the other not (chef 18-1). both lines have a stable diploid mode and mean of 22 with 10 pairs of homologous autosomes. the cell lines differ unambiguously in cloning ability in methylcellulose, colony morphology, and tumorigenicity; the expression of these traits was examined in a set of 18-1 x 16-2 hybrid clones. the results show initial suppression of tumorigenicity and ... | 1978 | 694725 |
| evidence against the existence of real isozymes of hypoxanthine phosphoribosyltransferase. | a method for reducing the degree of heterogeneity in the electrophoretic enzyme activity pattern of hypoxanthine phosphoribosyltransferase preparations by incubation with a (magnesium) phosphoribosyl diphosphate substrate is described. hypoxanthine phosphoribosyltransferase was isolated from human erythrocytes and chinese hamster livers. a subunit molecular weight of 26000--27000 as reported by other authors was obtained for both enzymes by gel electrophoresis in the presence of dodecylsulfate. ... | 1978 | 710424 |
| increased tissue conductance and ion transport in guinea pig ileum after exposure to staphylococcus aureus delta-toxin in vitro. | prior studies had shown that staphylococcus aureus delta-toxin was able to inhibit water absorption in guinea pig ileum and to elevate the cyclic amp content of this tissue, but was unable to elicit certain cyclic amp-mediated changes in y-1 adrenal or chinese hamster ovary cells. because water movement passively follows the net movement of electrolytes in the gut, this study investigated the effect of delta-toxin on ion transport in guinea pig ileum maintained in vitro. the transmural potential ... | 1978 | 711309 |
| growth of infective forms of trypanosoma rhodesiense in vitro, the causative agent of african trypanosomiasis. | a new approach to the culture of african trypanosomes led to the growth of the infective forms of the causative agent of human african trypanosomiasis. infective cultures of trypanosoma rhodesiense were initiated and maintained in vitro on chinese hamster lung cells. by changing daily one-third of the hepes-buffered rpmi 1640 medium containing 20 percent fetal bovine serum, the trypanosome numbers increased to 3 x 10(6) to 5 x 10(6) cells per milliliter. after 80 days in vitro at 37 degrees c, t ... | 1978 | 715441 |
| mutagenicity and carcinogenicity of 8-mop/uva in cell cultures. | hgprt-deficient mutants of chinese hamster cells and human skin fibroblasts are selected with 6-thioguanine after treatment with the combination 8-mop and uva. calculation based on this study indicate that 1.7 x 10(-5) mutants will be induced in the epidermis per session of puva therapy. induction of cell-transformation was observed in c3h and 3t3 mouse cells. | 1978 | 719181 |
| surface functions during mitosis i: phagocytosis, pinocytosis and mobility of surface-bound con a. | phagocytosis, pinocytosis and the surface distribution of concanavalin a (cona) have been analyzed during mitosis in several mammalian cell lines. use of the bisbenzimidazole dye, hoechst 33258, for chromosome staining after gentle fixation made possible the rapid identification and correlation of mitotic phase with surface properties. phagocytosis of both opsonized and nonopsonized particles is markedly depressed in mitotic cells of the mouse macrophage cell line j774.1. the uptake of opsonized ... | 1978 | 719746 |
| aconitase (e.c. 4.2.1.3) mitochondrial locus mapped to human chromosome 22: studies with chinese hamster--human somatic cell hybrids. | three separate somatic cell fusions were made between chinese hamster lines and human lymphocytes containing (1) a 3/4 translocation, (2) an x/9 translocation, and (3) a 17/9 translocation. eleven independently derived hybrids showed that only human chromosome 22 was consistently present when human aconm was expressed and absent when human aconm was not expressed. these studies assign a gene for human aconm to chromosome 22, and are consistent with prior gene-mapping results. | 1978 | 728065 |
| genetic analysis of cell malignancy--evidence from somatic cell genetics. | when normal nontumorigenic cells are fused with tumorigenic cells some of the resulting hybrids are nontumorigenic in respect to their ability to grow in immune-deficient nude mice. comparison of the chromosome content of nontumorigenic with tumorigenic hybrids, as well as with the cells of tumors which develop from the latter, reveals that in normal human x tumorigenic chinese hamster hybrid crosses, two specific human chromosomes of the nontumorigenic parental line are very likely responsible ... | 1978 | 728675 |
| isolation of mutant mammalian cells altered in polyamine transport. | chinese hamster ovary and rat myoblast cells resistant to the toxic action of methylglyoxal bis guanylhydrazone (mgbg), an antimitotic agent and inhibitor of polyamine synthesis, have been isolated by single step selection. mutagenesis with ethyl methanesulfonate increases the recovery of the variants at least 30-fold. intracellular accumulation of mgbg is greatly reduced in resistant cells. this property is accompanied by a 99% decrease in the uptake of all three naturally occurring polyamines. ... | 1978 | 730773 |
| excorporation efficacy with ca-dtpa of 241am and 252cf in the skeleton, liver, and kidney of the rat and syrian and chinese hamsters: lack of correlation with the biological half-times. | 1978 | 734050 | |
| [electron microscopic study of mammalian somatic cell fusion under the action of polyethylene glycol]. | an electron microscope study of heterokaryons and synkaryons, obtained after the treatment of suspension of human embryonic fibroblasts and cultured fibroblasts of chinese hamster (clone m151) with polyethylene glycol (pec, m. v. 6000) has shown that in 15 minutes after the administration of peg, the cell agglutination and disappearance of plasma membranes between cells takes place. it is not obvious, however, how per is passing through the cell envelope, but the similar action of pef towards th ... | 1978 | 734774 |
| assignment of the gene for dipeptidase 2 to mus musculus chromosome 18 by somatic cell hybridization. | evidence is presented for the assignment of the gene for dipeptidase 2 to mus musculus chromosome 18 by synteny testing and karyotypic analysis of chinese hamster x mouse somatic cell hybrid clones. dip-2 and chromosome 18 were expressed concordantly in 24/24 clones examined (ten primary clones and 14 secondary clones). synteny testing indicated that dip-2 was not expressed concordantly with the expression of any marker enzymes. | 1978 | 736882 |
| responses of mammalian metaphase chromosomes to endonuclease digestion. | digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and dnase ii) under optimal digestion conditions followed by giemsa staining produces sharp banding patterns identical to g-bands. in 3h-thymidine labeled, synchronized metaphase cells of the chinese hamster (cho line), the band induction is accompanied by the removal of dna. the single strand specific nuclease s1 and dnase i do not produce such banding patterns. | 1978 | 743903 |
| cytogenetic effects of inhaled ozone. | we have repeated as closely as possible the experiments of zelac et al., who observed significantly elevated levels of chromosome aberrations in short-term cultures of peripheral lymphocytes from chinese hamsters that had inhaled ozone. unlike zelac et al., we observed no increase in chromosome-type aberration levels, though a small increase in chromatid-aberration levels similar to that reported for exposed human subjects by merz et al. was seen. no increase in the levels of any chromosomal abe ... | 1978 | 745619 |
| sister-chromatid exchanges induced in rabbit lymphocytes by 2-aminofluorene and 2-acetylaminofluorene after in vitro and in vivo metabolic activation. | because short in vitro treatments of chinese hamster cells with 2-aminofluorene and 2-acetylaminofluorene did not increase sces even in the presence of a metabolic activation system, experiments were carried out with rabbits to see if in vivo activation occurred. rabbits injected with 2-af could activate the compound and a transient dose-dependent increase in sces was found in peripheral lymphocytes cultured at various times after the injection. with 2-aaf, however, the response was more variabl ... | 1978 | 745622 |
| cellular and molecular studies on globin gene expression. | globin gene expression has been studied with the use of a combination of cell and molecular biology techniques. with a somatic cell hybrid between mouse erythroleukemia (mel) and human erythroid cells, human and mouse globin genes can be coexpressed in the same hybrid cell. somatic cell hybridization between mel cells and nonerythroid cells (e.g., human fibroblasts) results in hybrid cells that cannot be induced to produce hemoglobin of any type. molecular hybridization of cellular dna and rna w ... | 1978 | 748760 |
| assignment of a human galactose-1-phosphate uridylyltransferase gene (galt1) to chromosome 9 in human-chinese hamster somatic cell hybrids. | 1978 | 752475 | |
| toward the development of a standard reference cholera antitoxin. | the need for a reference cholera antitoxin to serve as a standard for the calibration of cholera enterotoxin and toxoid as well as for measurement of the antitoxin response in animals and patients was recognized by the nih cholera advisory committee, niaid, dhew, usa. two cholera antitoxins have been used for several years as provisional references, but neither was considered to embody all of the properties of an ideal standard. accordingly, a lot of cholera antitoxin was prepared by immunizatio ... | 1978 | 753669 |
| [in vitro effect of asbestos fibers on short-term cultures of human lymphocytes]. | the endocellular incorporation of asbestos fibres seems to be capable to induce on chinese hamster cell cultures numerical and structural chromosome aberrations. in the present study asbestos fibres have been added at different concentrations to human cultured lymphocytes which have been harvested at 48 and 72 h, respectively. the results do not show significant difference in the distribution of chromatid and chromosome-type aberrations between the control cultures and the cultures treated with ... | 1978 | 755418 |
| assignment of the genes for triose phosphate isomerase to chromosome 6 and tripeptidase-1 to chromosome 10 in mus musculus by somatic cell hybridization. | evidence is presented for the assignment of the gene for triose phosphate isomerase to mus musculus chromosome 6 and tripeptidase-1 to chromosome 10 by synteny testing and chromosome assignment in chinese hamster x mouse somatic cell hybrid clones. neither tpi nor trip-1 were expressed concordantly with any known isozyme markers in 45 hybrid clones (13 primary and 32 secondary). karyotypic analysis of 21 clones showed that the expression of tpi and chromosome 6 were concordant in all cases as wa ... | 1978 | 566475 |
| assignment of a mus musculus gene for triosephosphate isomerase to chromosome 6 and for glyoxalase-i to chromosome 17 using somatic cell hybrids. | chinese hamster x mouse hybrid cells segregating mouse chromosomes have been used to assign a gene for triosephosphate isomerase (tpi-1, ec 5.3.1.1, mckusick no. 19045) to mouse chromosome 6, and a gene for glyoxalase-i (glo-1, ec 4.4.1.5, mckusick no 13875) to mouse chromosome 17. the genes for tpi-1 and lactate dehydrogenase b are syntenic in man and probably so in the dog. it is therefore likely that they are syntenic also in the mouse. it is of interest then that there is a mouse gene, ldr-1 ... | 1978 | 566476 |
| mutagenesis by n-acetoxy-2-acetyl aminofluorene of chinese hamster v79 cells is unaffected by caffeine. | 8-azaguanine (azg)- and 6-thioguanine (tg)-resistant cells (mutants) were induced in chinese hamster v79-4 cells by 0.1--2.5 microgram/ml n-acetoxy-2-acetyle aminofluorine (acaaf) treatments in the presence of 5% fetal bovine serum (fbs). the frequency of resistant colonies increased from 1 to 47 per 10(5) survivors. the effect of caffeine (50--200 microgram/ml) during the mutagenesis expression period was determined by adding caffeine 1--24 h after acaaf. the medium was replaced after 48 h expo ... | 1978 | 566636 |
| the action of af 2 on cultured hamster and human cells under aerobic and hypoxic conditions. | af 2 (2-(2-furyl)-3-(5-nitro-furyl)acrylamide) was toxic to chinese hamster v 79 cells and normal human fibroblasts in aerobic media. however, the toxicity of the drug was increased many times by hypoxia. similarly, the frequency of af 2-induced azaguanine- and ouabain-resistant mutants of v 79 cells was much higher in hypoxia than under aerobic conditions. both hamster v 79 cells and human fibroblasts metabolized af 2 and other nitrofurans rapidly only under hypoxic conditions. human fibroblast ... | 1978 | 566638 |
| time and cell systems as variables in fusion experiments with polyethylene glycol. | cell hybridization was done between a monolayer of b14-150 chinese hamster cells and a suspension of either mouse leukemia cells or normal human lymphocytes. cell contact was obtained by centrifugation of the suspension cells onto the monolayer cells in a culture plate. cell fusion was done by means of polyethylene glycol (peg). the optimum time for peg exposure as well as the yield of hybrid cells differed markedly with the different combinations. | 1978 | 566713 |
| properties of the thymidine transport system of chinese hamster ovary cells as probed by nitrobenzylthioinosine. | the transport of thymidine into chinese hamster ovary cells grown in suspension culture was measured under conditions in which thymidine was not metabolized, namely, when cells had been depleted of atp. the system transporting thymidine was saturable (kztm = 70 micron), rapid 50% of transmembrane equilibrium level attained within 8 sec), and was apparently shared by other nucleosides, but not thymine or hypoxanthine. 6([4-nitrobenzyl]thio)-9-beta-d-ribofuranosylpurine, "nitrobenzylthioinosine", ... | 1978 | 568178 |
| assignment of the gene for glyoxylase i to mouse chromosome 17 by somatic cell genetics. | evidence is presented for the assignment of the gene for glyoxylase i to mouse chromosome 17 using mouse x chinese hamster somatic cell hybrids. glo i was not expressed concordantly with any known marker enzymes which represented 11 linkage groups. the presence of chromosome 17 and expression of glo i were concordant in 31/31 clones. glo i is thus linked to the h-2 histocompatibility locus in the mouse. | 1978 | 569476 |
| replicon sizes in mammalian cells as estimated by an x-ray plus bromodeoxyuridine photolysis method. | a new method is described for estimating replicon sizes in mammalian cells. cultures were pulse labeled with [3h]thymidine ([3h]tdr) and bromodeoxyuridine (brdurd) for up to 1 h. the lengths of the resulting labeled regions of dna, lobs, were estimated by a technique wherein the change in molecular weight of nascent dna strands, induced by 313 nm light, is measured by velocity sedimentation in alkaline sucrose gradients. if cells are exposed to 1,000 rads of x-rays immediately before pulse label ... | 1978 | 570067 |
| the incorporation of homologous and heterologous hypoxanthine-guanine phosphoriboxyltransferase into mutant cells. | experiments are described leading to partial compensation of a deficiency in the enzyme hypoxanthine-guanine phosphoribosyltransferase in mutant cells by supplying the cells with exogenous purified enzymes. deae-dextran is an effective helper agent, whereas poly (l-lysine), lysolecithin and amphotericin b seem to inhibit the entry of the enzymes of their activity. enzyme preparation from chinese hamster was found to have different effects in different mutant cell lines. in mutant chinese hamster ... | 1978 | 563735 |
| mutagenicity studies with praziquantel, a new anthelmintic drug, in mammalian systems. | praziquantel, a new anthelmintic drug with antischistosomal and anticestodal properties, was tested in comparison with a placebo control and a 'positive control' with cyclophosphamide in mammalian test system in vivo for potential mutagenic effects. the test systems used and the tested doses of praziquantel were: (1) dominant lethal test on male nmri mice, 12 mating periods of 4 days each, 1 x 1200 mg/kg bw by mouth; (2) dominant lethal test on female nmri mice, treatment during pre-estrus, 1 x ... | 1978 | 580366 |
| effects of asbestos on epithelioid cell lines. | the in vitro effects of asbestos fibers on the growth and viability of cho cells, an epithelioid cell line derived from chinese hamster ovary, and on k-22 cells, an epithelial cell line derived from rat liver, have been studied. relatively low concentrations of asbestos (10 micrograms/ml) were quite cytotoxic to both cell types. a sample of chrysotile asbestos was more toxic than samples of crocidolite or amosite. the toxic factor could not be extracted from the asbestos and toxicity occurred on ... | 1978 | 233816 |
| the effect of bromodeoxyuridine on the segregation of the chicken-specific hprt gene from chinese hamster-chick red blood cell somatic hybrids. | the segregation of the chick-specific hprt gene was studied in three chinese hamster-chick red blood cell hybrid lines. the three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. bromodeoxyuridine becomes incorporated into the nuclear dna and increases the spontaneous segregation rate. | 1978 | 299650 |
| gamma-glutamyltransferase in human diploid fibroblasts and other mammalian cells. | gamma-glutamyltransferase was determined in wi-38 human diploid fibroblasts and compared to enzyme levels determined in several other mammalian cell lines including: fibroblast-like cells from human skin, tibia and foreskin; epithelial-like cells from human, bovine and monkey kidney; and transformed cells (chinese hamster ovary, hela s3 and sv-40 transformed wi-38). transformed cells had the lowest activity found followed in increasing order by fibroblasts, human and bovine epithelial cells and ... | 1978 | 27446 |
| interspecies interactions of arboviruses. iii. competition for virus envelope antigens in mixed getah and sindbis virus populations. | interactions of getah and sindbis virus populations in mixed infections of 5 tissue systems were competitive or neutral. the type of interaction is regulated by the host cell and virus population density. on reproduction in mouse brains and spev cell cultures the mixed population exhibited antigenic markers of getah virus; in syrian hamster kidney and chick embryo cell cultures those of sindbis virus; and in chinese hamster kidney cell cultures the antigenic markers of both viruses. mixed popula ... | 1978 | 29465 |
| growth of infective forms of trypanosoma (t.) brucei on buffalo lung and chinese hamster lung tissue culture cells. | infective cultures of trypanosoma (t.) brucei (strain 427) have been initiated and maintained on chinese hamster lung tissue culture cells and buffalo lung tissue culture cells. by changing daily one-third of the rpmi-1640 plus 20% fetal bovine serum medium, the cell numbers can be maintained at 2--4 x 10(6) cells/ml. the cultured trypanosomes on these two tissue culture cell types were infective to mice and morphologically similar to bloodstream slender trypomastigotes in having a subterminal k ... | 1978 | 31773 |
| human bronchus-mediated mutagenesis of mammalian cells by carcinogenic polynuclear aromatic hydrocarbons. | cultured human bronchial explants activated benzo[alpha]pyrene (bzap) into electrophilic metabolites that bind to dna in bronchial epithelial cells. promutagenic and mutagenic metabolites of bzap were also released into the culture medium. an increase in mutation frequency for ouabain resistance was found in chinese hamster v-79 cells when they were cocultivated with bronchial explants in the presence of bzap. the proximate carcinogenic form of bzap, the 7,8-diol [(+/-)-r7,t8-dihyroxy-7,8-dihydr ... | 1978 | 148049 |
| liver cell-mediated mutagenesis of mammalian cells by liver carcinogens. | a cell-mediated mutagenesis assay using primary cultures of rat liver cells and v79 chinese hamster cells has been developed. liver carcinogens and their structural analogues were studied. mutations in the v79 cells were characterized by resistance to ouabain. cocultivation of the liver cells and v79 cells in the presence of the carcinogens n-nitrosodimethylamine, n-nitrosodiethylamine, and aflatoxin b1 caused the induction of ouabain-resistant mutants of v79 cells. in the absence of liver cells ... | 1978 | 149308 |
| functional heterogeneity of transferrin-bound iron: iron uptake by cell suspensions from bone marrow and liver and by cell cultures of fibroblasts and lymphoblasts. | according to the hypothesis of fletcher and huehns, functional differences exist between both iron-binding sites of transferrin. the site designated a should mainly be involved in the delivery of iron to erythroid cells, whereas site b should donate its iron preferentially to cells involved in the absorption and storage of iron. in the present study this hypothesis could be confirmed by in vitro experiments with various cell types. iron transferrin preincubated with rat bone marrow cells donates ... | 1978 | 101008 |
| absence of mutagenicity of praziquantel, a new, effective, anti-schistosomal drug, in bacteria, yeasts, insects and mammalian cells. | praziquantel (embay 8440, droncit) a new, effective anti-schistosomal drug, was tested in various short-term assays that have shown a predictive value for the detection of potential carcinogens. indicator organisms s. typhimurium strains, s. pombe, s. cerevisiae, cultured v79 chinese hamster cells or human heteroploid cells and drosophila melanogaster were treated with praziquantel. the induction of reverse and forward mutations, mitotic gene conversions, x-linked recessive lethals, sister-chrom ... | 1978 | 106268 |
| [modified method of differential staining of sister chromatids]. | a modified method of obtaining differential staining of sister chromatids is described. it is simple, rapid, and effective, and at the same time inexpensive and accessible, since it allows one to use available reagents. when 5-bromdeoxyuridine was administered 24 hours before fixation into the chinese hamster cell culture the percentage of metaphases with a differential chromatid staining constituted 95--98, and when this substance was administered 28 hours before fixation into the human lymphoc ... | 1978 | 75748 |
| a new immunochemical method for the quantitative measurement of specific gene products in man-rodent somatic cell hybrids. | an immunochemical method has been developed for the quantitative determination of species-specific gene products, for instance alpha-galactosidase and n-acetyl-alpha-galactosaminidase, in man-rodent hybrid cells and in the parental cell lines. antisera raised against the purified enzymes are covalently coupled to sepharose 4b. the gene products are specifically removed from a cell lysate by incubating with the appropriate sepharose-coupled antiserum. after centrifugation followed by washing of t ... | 1978 | 83282 |
| molecular mechanisms involved in the production of chromosomal aberrations. i. utilization of neurospora endonuclease for the study of aberration production in g2 stage of the cell cycle. | chinese hamster ovary cells (cho) were x-irradiated in g2 stage of the cell cycle and immediately treated, in the presence of inactivated sendai virus, with neurospora endonuclease (e.c. 3.1.4.), an enzyme which is specific for cleaving single-stranded dna. with this treatment, the frequencies of all types of chromosome aberrations increased when compared to x-irradiated controls. these results are interpreted as due to the conversion of some of the x-ray induced single-stranded dna breaks into ... | 1978 | 83535 |
| thermal enhancement of the effectiveness of gamma radiation for induction of reproductive death in cultured mammalian cells. | the induction by gamma radiation of reproductive death in cultured cells derived from a rat ureter carcinoma (ruc-2) and from chinese-hamster lung tissue (ch-v79) was shown to be enhanced by hyperthermic treatments at 41, 43 and 45 degrees c. the degree of enhancement was found to depend on the line of cells studied, the temperature employed and the level of damage considered. the influence of accumulating sublethal damage was decreased by hyperthermia, and the final slope of the radiation survi ... | 1978 | 309451 |
| host range restriction of vaccinia virus in chinese hamster ovary cells: relationship to shutoff of protein synthesis. | chinese hamster ovary cells were found to be nonpermissive for vaccinia virus. although early virus-induced events occurred in these cells (rna and polypeptide synthesis), subsequent events appeared to be prevented by a very rapid and nonselective shutoff of protein synthesis. within less than 2 h after infection, both host and viral protein syntheses were arrested. at low multiplicities of infection, inhibition of rna synthesis with cordycepin resulted in failure of the virus to block protein s ... | 1978 | 310474 |
| shedding of the glycoprotein from vesicular stomatitis virus-infected cells. | in a culture of chinese hamster ovary cells infected with vesicular stomatitis virus, there is specific shedding of viral antigens into the medium. this shedding appears to be unrelated to progeny formation or to cell lysis. although all five of the virus-specific proteins are detected in the extracellular soluble fraction, the major antigen is the gs protein. this protein has a molecular weight of 54,000. indirect analysis of the content of sialic acid as well as peptide analysis of the gs and ... | 1978 | 211264 |
| assignment of the receptor for ecotropic murine leukemia virus to mouse chromosome 5. | the gene for the receptor for ecotropic murine leukemia virus (rev) has been assigned to mouse chromosome 5. this determination was made possible by an analysis of somatic cell hybrids between mouse and chinese hamster cells. the parents of these hybrids were a/hej or mus poschiavinus peritoneal exudate cells or balb/c primary embryo fibroblasts and e36, a chinese hamster lung fibroblast deficient in hypoxanthine guanine phosphoribosyltransferase. segregation of mouse chromosomes in these hybrid ... | 1978 | 212501 |
| cytogenetic studies of a simian virus 40 temperature-sensitive mutant-transformed chinese hamster cell clone at the permissive and nonpermissive temperature. | a clone of chinese hamster embryo cells transformed by tsa58, the temperature-sensitive mutant of simian virus 40, was analyzed for chromosome abnormalities at the permissive temperature (37 degrees c) and nonpermissive temperature (40.5 degrees c). trypsin-giemsa-banded metaphases were analyzed 1 week after the temperature shift. the metaphases from cells at both temperatures were pseudodiploid, with numerous chromosome changes primarily affecting chromosomes no. 1 and 2. other chromosomes (no. ... | 1978 | 212571 |
| simian virus 40 a gene function: dna content analysis of chinese hamster cells transformed by an early temperature-sensitive virus mutant. | replication of two chinese hamster embryo cell lines transformed by an early temperature-sensitive mutant of simian virus 40, tsa58, was examined by flow microfluorometry and autoradiography of [3h]thymidine-labeled cells in order to determine whether transformed cell dna synthesis is initiated by the virus a gene. at the permissive temperature (37 degrees), cells transformed by the mutant were like the wild-type virus transformants in appearance, colony-forming ability, high saturation density, ... | 1978 | 212747 |
| a herpes simplex virus 1 integration site in the mouse genome defined by somatic cell genetic analysis. | transfection experiments with hsv 1 in which one uses herpes simplex virus (hsv) thymidine kinase (tk) as a selectable prototrophic marker yield two classes of transformed cells: stable and unstable. in this report, we test the hypothesis that the stability phenotype can be explained by virus genome integration into a recipient cell chromosome. the method of analysis is by means of somatic cell genetics. we have isolated a series of microcell hybrids between a tk- chinese hamster cell line and a ... | 1978 | 214240 |
| localization of a gene for human alpha-galactosidase b (= n-acetyl-alpha-d-galactosaminidase) on chromosome 22. | the localization of the structural gene for human alpha-galactosidase b (= n-acetyl-alpha-galactosaminidase) was investigated by means of man-chinese hamster and man-mouse somatic cell hybrids. the hybrid clones were analyzed for chromosomes and for a large number of known enzyme markers. the lysates of the hybrid cells were treated with sepharose-coupled antihuman alpha-galactosidase b and the activity of the adsorbed enzyme was measured on the sepharose beads as n-acetyl-alpha-galactosaminidas ... | 1978 | 215508 |
| diphtheria toxin has the properties of a lectin. | the inhibition of protein synthesis in chinese hamster v79 cells by diphtheria toxin is antagonized by the lectins concanavalin a, succinylated concanavalin a, and wheat germ agglutinin but not by proteus vulgaris phytohemagglutinin or abrus agglutinin. the effects of concanavalin a and wheat germ agglutinin are reversed by methyl alpha-mannoside and n-acetylglucosamine, respectively. the inhibition of diphtheria toxin as a function of concanavalin a concentration fits a model of competitive inh ... | 1978 | 272642 |
| tumorigenicity and its suppression in cybrids of mouse and chinese hamster cell lines. | the effect of cytoplasm upon the expression of tumorigenicity was examined with a pair of mouse and a pair of chinese hamster cell lines, in intraspecies cybrids formed by reciprocal fusions between either tumorigenic or nontumorigenic cells and cytoplasms derived from them. with the mouse cells, 3t3 and the simian virus 40-transformed line svt2, the cybrid clones were tumorigenic when svt2 cells were fused with 3t3 cytoplasts, but not in the reciprocal fusion. with the hamster cells, chef/18 an ... | 1978 | 276880 |
| chimeric toxins: toxic, disulfide-linked conjugate of concanavalin a with fragment a from diphtheria toxin. | a disulfide-linked conjugate of concanavalin a (con a) and fragment a from diphtheria toxin has been synthesized and shown to be toxic for hela (human), chinese hamster ovary (cho), and sv3t3 (murine) cells. the conjugate was constructed by first coupling cystamine to con a with a carbodiimide reagent and then reacting the modified con a with reduced fragment a under conditions promoting disulfide interchange. the desired conjugate, obtained in nearly 50% yield relative to input of fragment a, w ... | 1978 | 281682 |
| inducers of dna synthesis present during mitosis of mammalian cells lacking g1 and g2 phases. | the cell cycle analysis of chinese hamster lung fibroblast v79-8 line by the premature chromosome condensation method has confirmed the absence of measurable g1 and g2 periods. sendai virus-mediated fusion of mitotic v79-8 cells with g1 phase hela cells resulted in the induction of both dna synthesis and premature chromosome condensation in the latter, indicating the presence of the inducers of dna synthesis above the critical level not only throughout s phase, as it is in hela, but also during ... | 1978 | 283413 |
| expression of "early" and "late" viral functions in a somatic cell hybrid between a mouse cell and a spontaneous yielder sv 40-transformed chinese hamster cell. | a somatic cell hybrid (cl. 6d) was originated from the fusion of mouse 3t3-4e) and spontaneous yielder sv 40-transformed chinese hamster (chk/svlp ag) cells. during the early stages of its history, the c1. 6d hybrid underwent a rapid chromosome loss, preferentially loosing hamster chromosomes. this was not a constant tendency of the hybrid cells. as the parental chk)svlp ag cells, the hybrid cells were always found 100 per cent sv40 t-antigen positive. while chk/svlp ag cells infectious sv 40 dn ... | 1978 | 204268 |
| direct fluorescent assay of urokinase and plasminogen activators of normal and malignant cells: kinetics and inhibitor profiles. | a direct rate assay for plasminogen activator has been developed using a synthetic fluorogenic peptide substrate, 7-(n-cbz-glycylglycylargininamido)-4-methylcoumarin trifluoroacetate. the assay correlates well with the standard 125i-labeled fibrin plate assay using highly purified urokinase, culture fluids from wi-38, chinese hamster vary or hela cells, or rous sarcoma virus-transformed chick fibroblasts as the source of plasminogen activator. the assay is sensitive, rapid, and linear throughout ... | 1978 | 204931 |
| cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer. | the chinese hamster genes for thymidine kinase (atp:thymidine 5'-phosphotransferase, ec 2.7.1.75) and galactokinase (atp:d-galactose 1-phosphotransferase, ec 2.7.1.6) have been cotransferred to mouse cells by chromosome-mediated gene transfer. hamster metaphase chromosomes were incubated with mouse b82 cells and 22 independent colonies were isolated in a selective medium. all of the 12 colonies analyzed expressed the donor form of thymidine kinase; the hamster form of galactokinase was also expr ... | 1978 | 204939 |
| assignment of the gene for adenosine kinase to chromosome 14 in mus musculus by somatic cell hybridization. | evidence is presented for the assignment of the gene for adenosine kinase to mus musculus chromosome 14 by synteny testing and karyotypic analysis of mouse x chinese hamster somatic cell hybrid clones. adok and two enzymes previously mapped to mouse chromosome 14, np and es-10, were expressed concordantly in 29 hybrid clones. chromosome analysis confirmed this assignment. syntenic evidence is also presented using several clones of a gene transfer system in which the gene for human hprt had integ ... | 1978 | 206412 |
| a host range difference between herpes simplex virus types 1 and 2. | the infectivities of several strains of herpes simplex virus type 1 and type 2 were measured by plating on established human and chinese hamster cell lines. whereas all the type 1 strains reproducibly showed a 10(4)-fold lower titre on the chinese hamster cells, the type 2 strains showed only a 10- to 100-fold lower titre. this host range difference has been used in the analysis of recombinants between type 1 and type 2 viruses. | 1978 | 206659 |
| specific changes in the oligosaccharide moieties of vsv grown in different lectin-resistnat cho cells. | the carbohydrate moieties of the g glycoprotein of vesicular stomatitis virus (vsv) grown in three distinct lectin-resistant (lecr) chinese hamster ovary (cho) cell lines have been compared by fine structural analysis of radiolabeled glycopeptides. the mutant wgariii, selected for resistance to wheat germ agglutinin (wga), produces vsv containing g glycoprotein specifically lacking in sialic acid. the mutant phari, selected for resistance to phytohemagglutinin (pha) and previously shown to lack ... | 1978 | 207434 |
| mutagenesis of chinese hamster cells in vitro by combination treatments with methyl methanesulfonate and n-acetoxy-2-acetylaminofluorene. | mutational synergism was examined in chinese hamster v79 cells exposed to methyl methanesulfonate followed by n-acetoxy-2-acetylaminofluorene (acaaf) at different time intervals. treatment with 500 micron methyl methanesulfonate resulted in 95% survival of cloning ability and induced approximately 4 azaguanine-resistant mutants/10(5) survivors. seven micron acaaf produced 10 times as many mutants, and the survival was 7%. lethal synergism was observed for methyl methanesulfonate treatments follo ... | 1978 | 208770 |
| localization of the binding site for cell attachment in the alpha1(i) chain of collagen. | certain cells such as cho (chinese hamster ovary) and fibroblasts attach to a substrate of type i collagen via proteins that link the cell surface to collagen. serum contains a glycoprotein, c-cap (collagen cell attachment protein), that can mediate this adhesion. previously, we established that alpha1(i)-cb7 (cyanogen bromide peptide 7), which lies within residues 552 to 822 of collagen, contains a major binding site for c-cap(alpha1(i)-cb7 has been renumbered to account for an additional tripl ... | 1978 | 209046 |
| aanlysis of dcmp deaminase and cdp reductase levels in hamster cells infected by herpes simplex virus. | several enzymatic activities involved in the biosynthetic pathways of nucleotides, including thymidine kinase, which has been used as a biochemical marker in studies of gene transfer, are induced by herpes simplex virus (hsv). the utility of additional markers prompted us to reanalyze the effects of hsv infection on the activities of two other enzymes for which direct selective methods can be devised: dcmp deaminase and cdp reductase. for this purpose, mutant chinese hamster (la1) cells devoid o ... | 1978 | 209209 |
| molecular dosimetry of the chemical mutagen ethyl methanesulfonate in escherichia coli and in v-79 chinese hamster cells. | 1978 | 209321 | |
| homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p. | it is possible to generate interspecific somatic cell hybrids that preferentially segregate mouse chromosomes, thus making possible mapping of mouse genes. therefore, comparison of the linkage relationships of homologous genes in man and mouse is now possible. chinese hamster x mouse somatic cell hybrids segregating mouse chromosomes were tested for the expression of mouse enolase (eno-1; ec 4.2.1.11, mckusick no. 17245), 6-phosphogluconate dehydrogenase [pgd; ec 1.1.1.44, mckusick no. 17220], p ... | 1978 | 209463 |
| new region of the simian virus 40 genome required for efficient viral transformation. | viable mutants of simian virus 40 with deletions in three regions of the virus genome (map coordinates 0.21-0.17, 0.59-0.54, and 0.67-0.74) have been tested for their ability to transform rat fibroblasts to anchorage independence. only those mutants whose deletions occur between 0.59 and 0.55 in the proximal part of the early region are defective in transforming ability. the most severely defective of these transform with less than one-hundredth the efficiency of wild type. they retain their def ... | 1978 | 209467 |
| reexpression of hprt activity following cell fusion with polyethylene glycol. | polyethylene glycol-1000 (peg-1000) induced fusion of hprt (e.c. 2.4.2.8) deficient chinese hamster cells with alpha-galactosidase a (e.c. 2.3.1.22) deficient cells from a patient with fabry's disease yielded hybrids which contained both human and hamster hprt, g6pd (e.c. 1.1.1.49), and aprt (e.c. 2.4.2.7) and chinese hamster alpha-galactosidase b. thus peg-1000 mediated somatic cell fusion led to reexpression of chinese hamster hprt. it did not restore the expression of human alpha-galactosidas ... | 1978 | 209782 |
| study on the mutagenicity of conjugated estrogens in human, animal and bacterial systems. | conjugated estrogens were studied for mutagenicity (and implied carcinogenicity) in two strains of s. typhimurium, two normal and three neoplastic human lymphoid cell lines (the latter from burkitt's lymphoma patients) in cell culture and a chinese hamster cell line in diffusion chambers implanted into mice. no significant increase in mutation rate or chromosome aberrations were noticed after exposure to conjugated estrogens. | 1978 | 217940 |
| persistence of the fc cytophilic antibody receptor in macrophage hybrid cells. | hybrid cell clones were obtained from mouse macrophase/ehrlich ascites cell fusions which showed the 'fc' cytophilic antibody receptor site, phagocytosis and motility typical of macrophages. with continued growth of the clones for 11 weeks, the receptor became undetectable but could be unmasked by dilute trypsin treatment; subsequent remasking of the receptor could be prevented with cycloheximide treatment of the hybrid cells. these hybrids produced cystic anaplastic tumours in neonatal irradiat ... | 1978 | 400978 |
| mutations of chinese hamster somatic cells from 2-deoxygalactose sensitivity to resistance. | in chinese hamster somatic cells, the spontaneous change of phenotype from 2-deoxygalactose sensitivity to resistance was studied using fluctuation test experiments à la luria and delbrück (1943) for four chinese hamster cell strains derived from v79. the results are consistent with true mutational events. the mutation rates are in the range of 1 to 3.5 x 10(-5) per cell per generation. the relationship between the 2-deoxyglactose resistance and the galactokinase markers is discussed. | 1979 | 488705 |
| oer and rbe for negative pion beams of different peak widths. | experimental data on survival curves for pion because of different peak widths under aerobic and hypoxic conditions are reported. metabolic depletion of oxygen by the chinese hamster cells (line v79) was used to obtain hypoxia. the results indicate that the rbe at the beam entrance (plateau) is approximately 1.0. when the bragg peaks were broadened to widths of 1.3, 7.8, and 10.5 cm (at the 80% dose level), the rbe (50% cell survival) at the peak centres was 1.7, 1.6, and 1.2, respectively. the ... | 1979 | 465928 |
| [pathology of mitosis after cellular recovery from a metaphase block]. | the experiments in chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. by the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. this form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis. | 1979 | 465693 |
| the metabolically mediated dna damage and subsequent dna repair by 4-chlorobiphenyl in chinese hamster ovary cells. | the metabolism of [3h]-4-chlorobiphenyl (cb) by chinese hamster ovary cell cultures resulted in the formation of lipophilic hydroxylated metabolites, trichloroacetic acid soluble conjugates and covalent macromolecular adducts. the major lipophilic metabolite was chromatographically similar to 4'-chloro-4-biphenylol. the intracellular macromolecules were fractionated into protein, rna and dna. although protein was the major target of covalent interaction with [3h]-cb and contributes 85% of the ma ... | 1979 | 451338 |
| nuclear protein synthesis at permissive and non-permissive temperatures in a chinese hamster ovary cell line with a temperature-sensitive defect in cytoplasmic non-mitochondrial protein synthesis. | 1979 | 446528 | |
| effects of hyperthermia (41.5 degrees) on chinese hamster ovary cells analyzed in motisis. | the mitotic cells from an asynchronous population of chinese hamster ovary cells exposed to 41.5 degrees for 7 hr were examined by light and electron microscopy to determine if there were any morphological abnormalities related to cell death or lengthening of metaphase induced by hyperthermia. all components of the mitotic apparatus were formed during exposure to heat, and the mitotic apparatus was functional as demonstrated by eventual cell division. however, heat caused the nuclear envelope to ... | 1979 | 445391 |
| interaction between radiation and drug damage in mammalian cells. iii. the effect of adriamycin and actinomycin-d on the repair of potentially lethal radiation damage. | we have studied the effects of actinomycin-d (amd) and adriamycin (adrm) on the repair of radiation damage in chinese hamster cells (v79) in plateau phase growth. suppression of potentially lethal damage repair (pldr) was observed in the presence of non-toxic levels of amd and minimally toxic levels of adrm. the suppression of pldr by amd persisted as long as the drug was present. removal of amd was followed by prompt repair of potentially lethal injury suggesting that suppression of pldr by amd ... | 1979 | 314429 |
| rescue of marker phenotypes: effects of budr sensitization, hypoxia and high let. | the survival curve of colony-forming ability of chinese hamster wg3h cells has been compared with the dose-response curve for the expression of an active thymidine kinase (tk) gene from these cells. the tk+ phenotype was measured by hybrid colony formation after fusion of wg3h (tk+) cells with chinese hamster a23 (tk-) cells. the tk+ survival data fitted a multi-target curve up to 3 krad of 137 cs irradiation, when a highly resistant fraction of hybrid colonies was seen at about 1 per cent survi ... | 1979 | 314426 |
| relationship between histidyl-trna level and protein synthesis rate in wild-type and mutant chinese hamster ovary cells. | a preliminary investigation was carried out to determine how conditional lethal mutants affected in particular aminoacyl-trna synthetases may be used to study the role of trna charging levels in protein synthesis. the relationship between rate of protein synthesis and level of histidyl-trna in wild-type cultured chinese hamster ovary cells was determined using the analogue histidinol to inhibit histidyl-trna synthetase activity. this response was compared with that obtained using a mutant strain ... | 1979 | 256567 |
| the induction of sister chromatid exchanges by dihydrodiols derived from 7,12-dimethylbenz[a]anthracene and 3-methylcholanthrene. | the in vitro induction of sister chromatid exchanges (sces) in chinese hamster ovary (cho) cells by the polycyclic hydrocarbons, 7,12-dimethylbena[a]anthracene and 3-methylcholanthrene and some of the related dihydrodiols was investigated. increased numbers of sces were seen in the chromosomes of cells exposed to non-k-region dihydrodiols. the most active compounds were the 3,4-dihydrodiol of 7,12-dimethylbenza[a]anthracene and the 7,8- and 9,10-dihydrodiols of 3-methylcholanthrene: the parent h ... | 1979 | 110440 |
| regulated synthesis of rna polymerase ii polypeptides in chinese hamster ovary cell lines. | rna polymerase ii polypeptides present in [35s]methionine-labeled chinese hamster ovary (cho) cell extracts have been quantitatively immunoprecipitated with an anti-calf thymus rna polymerase ii serum. analyses of the immunoprecipitates on sodium dodecyl sulfate polyacrylamide gels indicated that the immunoprecipitated polymerase ii of both wild type cho cells and the alpha-amanitin-resistant mutant ama1 had polypeptides of molecular weight 214,000, 140,000, 34,000, 25,000, 23,000, 20,500, and 1 ... | 1979 | 438182 |
| characterization of postribosomal aminoacyl-trna synthetases in cultured chinese hamster ovary cells. | 1979 | 454608 | |
| g1 phase chinese hamster v79-379a cells are inherently more sensitive to platinum bound to their dna than mid s phase or asynchronously treated cells. | 1979 | 475817 | |
| cell-mediated mutagenesis in cultured chinese hamster cells by polycyclic hydrocarbons: mutagenicity and dna reaction related to carcinogenicity in a series of compounds. | three polycyclic hydrocarbons, benz(a)anthracene, 3-methylcholanthrene and 7,12-dimethylbenz(a)anthracene, have been studied in a cell-mediated mutagenesis system using bhk 21 cells to metabolize the hydrocarbons and v-79 cells as targets for detecting induced cytotoxicity and mutation. in large-scale experiments, the dna of v-79 cells was analyzed by column chromatography to determine the nature and true extent of reaction of hydrocarbons with dexoyribonucleosides. products with dna formed by t ... | 1979 | 110710 |
| platinum-induced mutations to 8-azaguanine resistance in chinese hamster ovary cells. | 6 platinum (pt) compounds were compared in suspension cultured chinese hamster ovary (cho-s) cells with respect to their inhibition of growth, their reduction of cloning efficiency, and their induction of mutants resistant to 200 microm (30 micrograms/ml) 8-azaguanine (8-ag) and 3 mm ouabain (oua), respectively. the toxicity of these compounds can be ranked by the medium concentrations which decrease suspension growth/or cloning efficiency by 50%: cis-pt(nh3)2-cl2 (0.9/1.5 microm) greater than p ... | 1979 | 460295 |
| the cytotoxic, mutagenic and clastogenic effects of chromium-containing compounds on mammalian cells in culture. | examples of chromic and chromate salts have been examined for their effects on a cultured chinese hamster cell line. the responses studied were cytotoxicity, mutagenesis and clastogenesis. chromate (hexavalent chromium) salts of both high and medium water solubility were active in producing all three classes of response, whereas an insoluble chromate salt and a soluble chromic (trivalent chromium) salt were inactive. in addition to illustrating the value of using mammalian cells in culture for s ... | 1979 | 460294 |
| sister chromatid exchanges induced by inhaled anesthetics. | there is sufficient evidence that anesthetics may cause cancer to justify a test of their carcinogenic potential. baden, et al., using the ames test, a rapid and inexpensive genetic indicator of carcinogenicity, have shown that among currently used anesthetics fluroxene alone caused bacterial mutations. the authors used the sister chromatid exchange (sce) technique, another rapid assay of mutagenic-carcinogenic potential. the frequency of sister chromatid exchanges in chinese hamster ovary cells ... | 1979 | 453561 |
| enhancement of thermal killing by polyamines. iii. synergism between spermine and gamma radiation in hyperthermic chinese hamster cells. | 1979 | 451160 | |
| differential effect of procaine on irradiated mammalian cells in culture. | hela and v-79 chinese hamster cells temporarily stored in ampoules were treated with the local anesthetic procaine. postirradiation treatment increased lethality in hela cells depending on drug concentration, duration of treatment and cell density, as measured by colony-forming ability upon plating. if present during irradiation only, procaine protected from irradiation. in v-79 cells procaine potentiated radiation lethality only in freshly trypsinized cells. procaine effect was thus cell type s ... | 1979 | 441343 |
| effect of continuous insulin infusion with an implantable seven-day minipump in the diabetic chinese hamster. | 1979 | 436792 | |
| excision repair of cis-diamminedichloroplatinum(ii)-induced damage to dna of chinese hamster cells. | platinum was lost from the dna of cis-diamminedichloroplatinum(ii) [cis-pt(ii)]-treated exponentially growing chinese hamster v79-379a cells with a half-life of 28 hr. by contrast, platinum was lost from the dna of cells treated in stationary-phase culture with a half-life of 4 days. cells treated in and allowed to remain as a stationary-phase culture maintained an intact and apparently viable appearance. when the stationary-phase culture was diluted with fresh medium, cell division occurred, an ... | 1979 | 427811 |
| the relevance of a caffeine post-treatment to sce incidence induced in chinese hamster cells. | 1979 | 113671 |