Publications

immunoprecipitation of biosynthetically-labelled proteins from different papua new guinea plasmodium falciparum isolates by sera from individuals in the endemic area.	the human serum antibody response to plasmodium falciparum infection in papua new guinea has been studied by electrophoretic analysis of immunoprecipitated biosynthetically-labelled malaria proteins from three different isolates maintained in long-term in vitro culture. differences in protein antigenic composition in different lines have been described and simplified by examination of antigens recognized only by hyperimmune serum. an in vitro assay has been used to screen various human sera cont ...	1981	7033895
serum concentrations of chloroquine in a patient with a late recrudescence of kenyan plasmodium falciparum malaria.	a swedish tourist who had visited kenya fell ill with plasmodium falciparum malaria 11 days after returning home, in spite of taking pyrimethamine (50 mg weekly) as malaria prophylaxis. chloroquine treatment (25 mg base/kg body-weight) giving serum concentrations of 0.30 mumol/l cleared the patent parasitaemia and the patient recovered. recrudescence occurred, however, within 42 days. a second chloroquine course (30 mg base/kg) gave serum levels up to 1.28 mumol/l. the patient improved rapidly a ...	1981	7034310
doxycycline in the treatment of falciparum malaria among aborigine children in west malaysia.	doxycycline in a single dose was found to be a valuable drug in the treatment of chloroquine-resistant falciparum malaria. it was less effective in a single daily dose of 4 mg/kg body-weight for four days, when it cured only five out of nine patients, while a dosage of 4 mg/kg body-weight for seven days cured 23 out of 26 patients.	1981	7034311
imported malaria in a hospital in paris.	the number of cases of imported malaria diagnosed by the parasitology laboratory of the pitié-salpêtrière hospital group in paris has shown a significant increase in recent years. from 1970 to 1979, 443 cases were diagnosed and the annual number recorded during this period rose from seven to 93 cases. this is causing anxiety since plasmodium falciparum is the principal species observed, affecting two out of three patients. the principal epidemiological characters of malaria imported into france, ...	1981	7034314
the sickle cell trait in the santhals of ajodhya hills of purulia district, west bengal.		1981	7034322
congenital transfer of antibodies against malarial sporozoites detected in gambian infants.	a survey of the antibody response to sporozoites of plasmodium falciparum was carried out on plasma samples obtained from 20 mothers and their newborn infants living in the gambia, west africa. serological assays detected antisporozoite antibodies in 90% (18/20) of the mothers, and similar titers were detected in 17/18 of their infants. the antisporozoite antibodies were gradually lost from the circulation of the babies until by approximately 6 months of age positive reactions against p. falcipa ...	1981	7034558
competitive inhibition by soluble erythrocyte glycoproteins of penetration by plasmodium falciparum.	glycophorin, the major sialoglycoprotein of the erythrocyte membrane, was extracted from human erythrocyte ghosts by the lithium diiodosalicylate phenol (lis) or chloroform-methanol (cm) methods. the products (lisgp and cmgp) were examined for their capacity to inhibit invasion of erythrocytes by plasmodium falciparum in vitro. in the presence of either glycoprotein, parasitemia was significantly less than in control cultures, indicating competitive inhibition of attachment. desialylation result ...	1981	7034559
reaction of immune sera with components of the human malarial parasite, plasmodium falciparum.	human and monkey sera from individuals exposed to plasmodium falciparum were characterized by indirect immunofluorescence, in vitro parasite growth inhibition, and immunoprecipitation of 125i-labeled parasite antigens followed by analytical sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (sds-page). in general there was a good correlation between fluorescence titer and the ability of a serum to inhibit parasite growth in vitro. exceptions were found, however. some variance was seen in ...	1981	7034560
quinine alone versus quinine plus a pyrimethamine-sulfadoxine combination in the treatment of plasmodium faliciparum cerebral malaria.	fifty-two patients with severe chloroquine resistant plasmodium falciparum malaria were treated in a randomized double blind study with either quinine and a single dose of pyrimethamine-sulfadoxine (fansidar) or quinine alone. although no statistically significant differences were observed, the 25 patients who received both drugs responded faster and had a more favorable outcome (no deaths) when compared to the 27 who received quinine alone (2 deaths).	1981	7034564
antigenic structure and related aspects of the biology of plasmodia: the present situation: scientific working group on the immunology of malaria.	major progress has been made in the production of monoclonal antibodies against various plasmodium species and developmental stages. some of the monoclonal antibodies, especially those directed against sporozoites, have proved to be protective in rodent and simian systems. antigenic analysis has been advanced by the application of these monoclonal antibodies, and recent studies have concentrated on membrane-bound antigens.while promising leads have emerged from the in vitro production of viable ...	1981	7034986
malaria in malaysian soldiers, 1980.		1981	7035854
preliminary characterization of the major rna species from plasmodium falciparum.	polyacrylamide gel electrophoresis of the total rna extracted from cultures of plasmodium falciparum resolved two major rna components with estimated molecular weights of 1.3 x 10(6) and 0.72 - 0.74 x 10(6). oligo(dt)-cellulose column chromatography of the total cellular rna indicates that more than 90% of the total radiolabeled rna lacks substantial poly(a) sequences (poly(a)-). resolution of the poly(a)- fraction on polyacrylamide or agarose gels indicates that the majority of the poly(a)- rna ...	1981	7035940
interactions of plasmodium falciparum infected erythrocytes with ligand-coated agarose beads.	the binding of normal and plasmodium falciparum-infected squirrel monkey erythrocytes to columns of ligand-coated agarose beads was compared. concanavalin a, ricin, soybean and peanut agglutinin-coated beads retain erythrocytes containing large developmental stages of the parasite preferentially to ring-containing erythrocytes or to normal erythrocytes. binding is inhibited by the sugar corresponding to the lectin's specificity. preferential binding does not occur with wheat germ or ulex europae ...	1981	7035941
malaria in scotland and its presentation in a glasgow hospital.		1981	7028680
sensitive high-performance liquid chromatographic analysis for chloroquine in body fluids. application to studies of drug resistance in plasmodium falciparum.	a high-performance liquid chromatographic method has been developed for the sensitive determination of chloroquine in body fluids. the method has been applied to quality-control assay of world health organization (who) in-vitro, macro-test kits for the assessment of susceptibility of plasmodium falciparum to chloroquine. experiments utilizing [14c] chloroquine demonstrated that water was not capable of efficiently desorbing chloroquine from the inside surfaces of kit vials. the addition of blood ...	1981	7028768
an authentic case of pulmonary malaria caused by plasmodium falciparum.		1981	7028885
malaria prophylaxis.		1981	7029226
malaria in refugees from indo-china as seen australia in 1980.		1981	7029230
[in vitro culture of plasmodium falciparum, a new contribution to the antimalaria campaign].		1981	7029455
[plasmodium falciparum malaria resistant to polychemotherapy. usefulness of pathogen culture (author's transl)].	in a patients with p. falciparum malaria contracted in thailand, the course of the disease under treatment suggested resistance to both chloroquine and pyrimethamine-sulfadoxine, as well as reduced sensitivity to quinine. this was confirmed by in vitro tests on continuous culture of the strain. therapeutic success was obtained with a quinine-cycline combination. the problems raised by the emergence of p. falciparum strains resistant to polychemotherapy are emphasized.	1981	7029457
ovalocytic erythrocytes from melanesians are resistant to invasion by malaria parasites in culture.	ovalocytic erythrocytes from melanesians in papua new guinea have been demonstrated to be resistant to infection by malaria parasites (plasmodium falciparum) in culture by using a double-label fluorescence assay of merozoite invasion. that merozoites do not bind irreversibly to ovalocytes has been demonstrated by an assay that measures competition between ovalocytes and normocytes. analysis of behavior on thermal deformation has demonstrated that ovalocytes are more more thermostable than normoc ...	1981	7029547
malaria 'breakthroughs' and resistance to chloroquine in africa. case reports.	four cases of plasmodium falciparum malaria are presented. these cases are typical of chloroquine resistant malaria, with their pattern of 'breakthrough' malaria despite chloroquine prophylaxis, absence of response to therapeutic doses of chloroquine and recrudescence of the malarial parasites. these cases should alert physicians in other parts of the world, who may have to treat travelers from central and west africa, and particularly from the ivory coast, to the possibility that the malaria co ...	1981	7029733
enzyme typing of plasmodium falciparum from african and some other old world countries.	freeze-dried and freshly cultured isolates of plasmodium falciparum from different countries have been examined for variation in six enzymes, gpi, pgd, ldh, gdh, ada and pepe, using starch gel electrophoresis. variant forms of each enzyme occur. for most enzymes, similar forms occur in isolates from different parts of the world, although differences in frequencies of each form are found. ldh-2 has been found only in african isolates. pepe variants vary markedly in their frequency between east an ...	1981	7029800
enzyme typing of some isolates of plasmodium falciparum from thailand.	one hundred and eighty nine isolates of plasmodium falciparum collected in thailand, and eleven originating from cambodia, have been typed by starch-gel electrophoresis of six enzymes (gpi, ldh, gdh, pgd, ada, pepe). substantial polymorphism was found only with gpi. occasional variants occurred with ada, while the other four enzymes appeared to be invariant by the tests used. the results are compared with those of similar studies on african isolates, and lead to the provisional conclusion that p ...	1981	7029801
resistance of ten thai isolates of plasmodium falciparum to chloroquine and pyrimethamine by in vitro tests.	in vitro drug resistance tests of ten isolates of plasmodium falciparum from three different collection points in central thailand have been carried out, and the results compared with those of similar tests with a drug-sensitive west african isolate. judged by concentration of drug tolerated, the thai isolates appeared to be about 10 times as resistant to chloroquine, and usually about 10(5) times as resistant to pyrimethamine, as the african isolate. a little variation amongst the thai isolates ...	1981	7029802
a compact semi-automated continuous cultivation system for plasmodium falciparum.	a compact inexpensive semi-automated continuous cultivation system for plasmodium falciparum is described. the system is simple to operate and allows cultures to proceed unattended for several days. a particularly useful feature is a portable cultivation module that is freely transferable from the incubator to a tissue culture cabinet for manual operations such as sampling or addition of red cells. each cultivation module holds plastic flasks which can give a combined culture surface area of up ...	1981	7029804
plasmodium falciparum gametocytogenesis stimulated by lymphocytes and serum from infected gambian children.		1981	7029805
the rate of production of plasmodium falciparum gametocytes during natural infections.		1981	7029806
chloroquine-resistant falciparum malaria in madagascar and kenya.	3 african cases of chloroquine-resistent (ri) p. falciparum malaria, proved by recrudescences after administration of recommended doses and adequate serum levels of chloroquine, are described. the 3 patients, swedish women aged 43, 27, and 41, had never visited areas where chloroquine-resistent p. falciparum is known to exist. 2 patients had taken regular prophylaxis with chloroquine, while the 3rd had interrupted chloroquine use with temporary use of pyrimethamine. all 3 were treated with ch ...	1981	7030238
malaria.		1981	7030441
[plasmodium falciparum and drepanocytic gene in popular republic of congo. i. prevalence of malaria and drepanocytic trait among school children in brazzaville area (author's transl)].	from 1978 to 1979, 5 surveys, among schoolchildren, were carried out during the rainy season in the neighbourhood of brazzaville (r.p. congo): 3 in pk 45 village (northern part of the capital), 2 in djoumouna village (southern part), and 1 in "talangai" (a suburb of the capital). 868 exams (plasmodic and splenic index fitted with hemoglobin composition [hb aa or hb as]) were done. it appeared that 19,6% of schoolchildren examined were heterozygous sicklers (as). this percentage confirmed the pre ...	1981	7031402
[plasmodium falciparum and drepanocytic gene in popular republic of congo. ii. clinical aspects of malaria according to parasitemia and hemoglobin genotype (author's transl)].		1981	7031403
complete in vitro maturation of plasmodium falciparum gametocytes.		1981	7031476
clones of the malaria parasite plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes.	a culture line of plasmodium falciparum (fcr-3/gambia) was used to select and place in culture cells containing a single parasite. the method depends on examination of minute droplets of dilute cell suspensions with oil immersion phase-contrast microscopy. droplets found to contain a single parasite were maintained under appropriate culture conditions until detectable numbers of parasites were present (generally by day 21). of nine clones that grew up, seven were knobless and two were knobby. th ...	1981	7031656
fibrin-degradation products in falciparum malaria.	a fatal female case of cerebral falciparum malaria who was accidentally, artificially and directly infected in japan through nursing an imported falciparum malaria was experienced. these observations raised a question as to whether the disseminated intravascular coagulation (dic) would occur in falciparum malaria. then, 84 congolese patients with uncomplicated falciparum malaria were studied on the coagulation. serum fibrin-degradation products (fdp) levels were only slightly raised (10-40 micro ...	1981	7032150
chloroquine sensitivity of plasmodium falciparum in ibadan, nigeria: ii. correlation of in vitro with in vivo sensitivity.	plasmodium falciparum malaria was treated in 82 children with 25 mg/kg chloroquine orally over three days. they were observed for 28 days during which blood films were examined periodically for malaria parasites. asexual forms of p. falciparum, present in the blood films of all the patients before commencing treatment, disappeared rapidly and by the third day no parasites were seen in blood films from any of them. among the patients observed for more than three days, blood films remained negativ ...	1981	7036425
fansidar resistant falciparum malaria acquired in south east asia.	a case of plasmodium falciparum malaria resistant to fansidar (sulphadoxine plus pyrimethamine) at a level corresponding to r iii and resistant to chloroquine is reported. the infection was most certainly acquired in malaysia, but diagnosed and treated in a non-malarious area. normal resorption and elimination rates of the fansidar components excludes cure failure due to abnormal drug fate in the host. p. falciparum parasites from the patient have been maintained in vitro cultures. the patient w ...	1981	7036431
the in vivo sensitivity of plasmodium falciparum to chloroquine and to sulphadoxine-pyrimethamine combination in ibadan, nigeria.	fifty-eight nigerian children with plasmodium falciparum malaria were allocated randomly into two groups and treated with either chloroquine (25 mg/kg over three days) or fansidar (35 mg sulphadoxin (+ 1/20 pyrimethamine) per kg single dose)). they were observed for 28 days during which blood films were examined periodically for malaria parasites. asexual forms of p. falciparum, which were present in the blood films of all the patients in both groups before commencing treatment, disappeared rapi ...	1981	7036438
s-antigens and isozymes in strains of plasmodium falciparum.	evidence was found for the independent reassortment of parasite genes for s-antigens and isozymes. the lagos and palo alto strains of plasmodium falciparum had the same isozyme forms of pgd and ldh but different s-antigens. in contrast, the bw (gambia) and palo alto strains had different isozyme forms of gdh but shared some s-antigens. from artificial mixtures of parasites with different isozymes of gdh and different s-antigens, clones were derived in vitro which had a single isozyme type of gdh ...	1981	7036439
malaria blood cultures: a rapid fixation method to prevent sloughing of the thick film.		1981	7036441
[origin and incidence of the sickle cell gene in the republic of panama].		1981	7036238
[plasmodium falciparum malaria and sickle cell gene in the popular republic of congo. i. relationship between parasitemia and sicke cell trait in djoumouna (region of brazzaville) (author's transl)].	the relationship between sickle cell trait and falciparum malaria was studied in the village of djoumouna, twenty kilometers south west of brazzaville. malaria is characterized by a stable high intensity of transmission on the average one infective mosquito bite by night and by child contrasting with a relatively low malarial infection rate. the prevalence of carriers of an s gene (as) does not change with age: 22.2% for children under 5 years, 22.1% for childrern between 5 and 15 years, and 22. ...	1981	7036841
growth of human malaria parasites in biotinylated erythrocytes.	the adaptation of the biotin-avidin system for the analysis of membrane pathobiology in plasmodium falciparum malaria is described. biotin was linked covalently via the succinimide ester derivative (biotinyl-n-hydroxysuccinimide ester, bnhs) to intact human erythrocytes, prior to inoculation and in vitro cultivation of falciparum parasites. growth experiments indicated that incubation concentrations of less than 1.0 mg bnhs/1.0 ml erythrocyte packed cell volume could yield biotinylated erythrocy ...	1981	7038477
partial purification and characterization of lactate dehydrogenase from plasmodium falciparum.	lactate dehydrogenase (ldh) from plasmodium falciparum was partially purified by two different procedures. in the first procedure, parasitized erythrocytes (80% parasitemia) were lysed, and the soluble fraction was purified on deae-sephadex to separate the parasite ldh(ldh-p) from the ldh isoenzymes present in the human erythrocytes. ldh-p was then purified by high-performance liquid chromatography (hplc) on a tsk-g-3000 sw protein column. this two-step procedure gave ldh-p with specific activit ...	1981	7038478
isolation and characterisation of ribosomal rna from the human malaria parasite plasmodium falciparum.	ribosomal rna isolated from the malaria parasite plasmodium falciparum consists of two species with molecular weight of 1.49 +/- 0.09 x 10(6) and 0.78 +/- 0.02 x 10(6) present in equimolar quantities. their molecular weights are comparable with those of other protozoa but quite distinct from those of the human host. the overall base composition of the rrna (40% g+c) is close to that of the rodent parasite plasmodium berghei, unlike the latter, however, p. falciparum has no nick in the rna from i ...	1981	7038480
[a new case of plasmodium falciparum malaria resistance to chloroquine in africa (rwanda) (author's transl)].	report of a case of p. falciparum malaria observed in rwanda. relapse and positive blood thick smear has been observed 8 days after the beginning of the treatment of the previous fit of fever by chloroquine. authors recall the resistance criteria fixed by o.m.s. for antimalarial drugs and question wether drug-resistant malaria is not spreading in east-africa, considering some similar cases reported in the region.	1981	7040906
acute malaria in children in madang: endemicity, clinical presentation and treatment.	much of the present knowledge of the status of malaria in papua new guinea stems from a few studies carried out in the past. in the present situation there are many areas of uncertainty not least the effects of poor acceptance of residual spraying programmes and the emergence of chloroquine-resistant falciparum malaria. new anti-malarials are being developed, new methods of vector control may become useful and immunization may soon become possible. in order to assess the impact of these measures ...	1981	7039178
chloroquine-resistant plasmodium falciparum malaria in madang children.		1981	7039179
inhibition of the in vitro growth of plasmodium falciparum by human polymorphonuclear neutrophil leucocytes.		1981	7039878
sulfadoxine-pyrimethamine resistant falciparum malaria in thai children.	sixty-eight children with uncomplicated falciparum malaria admitted to the hospital for tropical diseases in bangkok during april-december 1980 were randomly divided into 3 groups and given 3 regimens. group 1 of 27 cases were treated with a single dose of sulfadoxine 20 mg per kg body wt and pyrimethamine 1.0 mg per kg body wt. two cases (7.4%) were cured (s) while 4 cases (14.8%) showed ri failure, 17 cases (63.0%) rii failure and 4 cases (14.8) riii failure. in group 2, 18 cases were treated ...	1981	7043751
prophylaxis and management of malaria.		1981	7018479
[conditions for re-establishing the schizogonic rhythm of plasmodium falciparum in vitro].		1981	7018722
decrease in susceptibility of plasmodium falciparum to mefloquine in continuous culture.	chloroquine-resistant p. falciparum, line fck from thailand, was tested for susceptibility to mefloquine in continuous culture. the drug resulted in 50% schizont inhibition at the level of 8.0 nmol/ml of culture when tested for the first time. after three months of discontinuous exposure to mefloquine at increasing levels, the sensitivity of the parasite decreased. a concentration as high as 128 nmol/ml eventually was required for 50% inhibition of growth.	1981	7018728
cerebral malaria in adult zambian africans.		1981	7018881
impairment of the growth of plasmodium falciparum in hbee erythrocytes.	hbe is a beta-chain mutant frequently found among inhabitants of southeast asia and surrounding territories. we find that plasmodium falciparum multiplies more slowly in erythrocytes from individuals homozygous for hbe than in cells from hba individuals. in contrast, this parasite grows normally in erythrocytes heterozygous for hbe. this is the first direct evidence that suggests what has been suspected on the basis of circumstantial data, that hbe-containing erythrocytes might be advantageous t ...	1981	7019245
failure of chloroquine prophylaxis in plasmodium falciparum from east africa.		1981	7019473
malaria: are we prepared?		1981	7019474
malaria antibody titres as measured by the indirect fluorescent antibody test in relation to parasitaemia and treatment.	two hundred and two sera from orang asli patients living in malarious areas were tested by the washed-cell, thick smear malaria ifa test. these patients were infected with p.vivax, p.falciparum and some with both parasites. antibodies to the homologous antigens were detected at titres of 1 : 16-1 : 4096 about 60 days after onset of treatment. eighteen months after cure antibody levels had fallen to lower levels of reactivity.	1981	7020094
chloroquine resistant plasmodium falciparum infection from lampung and south sumatra, indonesia.	a report was made of 4 cases of chloroquine resistant plasmodium falciparum infections. the infections, detected in jakarta, were imported from kotabumi, tanjung karang, the island of pidada in the lampung province and from pangkalpinang on the island bangka in the province of south sumatra. treatment with courses of 1500 mg chloroquine base and with increased dosages up to 2250 mg base failed to cure the patients. the chloroquine sensitivity test in vitro was carried out in 3 patients, which sh ...	1981	7020096
neonatal malaria in southern thailand.		1981	7020097
malaria in cape town. a report of 3 cases and a review of current therapy and prophylaxis.	three cases of malaria seen within 1 week at the somerset hospital in cape town are reported. one of these patients developed cerebral malaria and severe brain damage. the management of acute malaria and its complications, as well as the prophylaxis, is briefly reviewed. in view of the difficulty in obtaining intravenous quinine in cape town, it is strongly recommended that small supplies of intravenous quinine be maintained at centres throughout the country. this may decrease the incidence of c ...	1981	7020110
malaria in children.	the incidence of malaria in the united states has tripled during the past decade. a high index of suspicion for this diagnosis is warranted in travelers, visitors and refugees who have persistent fever. the possibility of malaria in children among these groups should not be overlooked. a common cause of malaria in travelers returning to the united states from endemic areas is failure to complete the appropriate course of chemoprophylaxis. except in plasmodium falciparum infections, treatment sho ...	1981	7020395
clinical drug-resistant falciparum malaria acquired from cultured parasites.	this is the first reported infection with plasmodium falciparum acquired from continuous cultures of these parasites. the strain involved, fcr3, was originally isolated from the gambia, west africa, and was initially sensitive to chloroquine in vitro. after nearly 4 years of continuous culture without chloroquine pressure, it became resistant to chloroquine in vitro and in vivo, as demonstrated by the failure of both prophylactic and therapeutic regimens to control an accidental inoculation of p ...	1981	7020443
prevalence of chloroquine-resistant falciparum malaria in the brazilian amazon.	the prevalence of chloroquine-resistant falciparum malaria was determined for humans living at 28 different sites in the brazilian amazon. blood samples obtained from each patient were defibrinated, placed in vials containing 0.5% glucose and or chloroquine and incubated for 24 hours at 39-40 degrees c without agitation. in vitro sensitivity of the parasite to four different concentrations of chloroquine was determined for each sample. after 24 hours of incubation, trophozoites of plasmodium fal ...	1981	7020444
antimalarial activity of quassinoids against chloroquine-resistant plasmodium falciparum in vitro.	the growth of plasmodium falciparum in vitro was markedly inhibited by certain quassinoids (the bitter principles from plants of the family simaroubaceae). the most active compound, simalikalactone d, gave complete inhibition at 0.005 microgram/ml. glaucarubinone an soularubinone were equally effective at 0.006 microgram/ml, whereas chaparrinone and simarolide had little effect even at 0.01 microgram/ml. these relative activities are parallel to the antineoplastic activities of these materials.	1981	7020445
infectivity of a strain of plasmodium falciparum from hainan, people's republic of china, to different anophelines.		1981	7020446
red cells containing hemoglobin e do not inhibit malaria parasite development in vitro.		1981	7020447
the enzyme-linked immunosorbent assay (elisa) for malaria. iii. antibody response in documented plasmodium falciparum infections.	serum specimens from patients in el salvador, central america, with slide-proven plasmodium falciparum infections were examined for antibodies to p. falciparum using the enzyme-linked immunosorbent assay (elisa) and the indirect fluorescent antibody (ifa) methods. both serologic tests were positive in 78.1% of the 827 samples, both negative in 5.4%, the elisa positive alone in 6.3%, and the ifa alone in 10.2%. agreement between the serologic tests was better in the specimens with high positive t ...	1981	7020453
malaria.		1981	7020587
invasion of erythrocytes by malaria merozoites: evidence for specific receptors involved in attachment and entry.	invasion of erythrocytes by malaria merozoites involves attachment of the merozoite membrane at the point of collision with the erythrocytes, then reorientation of the merozoite such that its apex is opposed to the erythrocyte membrane, followed by invaginatin of the erythrocyte membrane and interiorization of the parasite. specific recognition of erythrocyte surface components by the merozoite has been shown by studies on the specificity of merozoites of different malaria species for a limited ...	1981	7021086
ascites production in the squirrel monkey (saimiri sciureus).	a method is described for inducing the production of large amounts of ascitic fluid (af) in the squirrel monkey saimiri sciureus. the total amount of protein in the induced af is close to 60% of that in the serum. electrophoretic analysis of serum and af samples from the same monkey revealed similar protein patterns, including gamma globulins. antibody titers against plasmodium falciparum in infected monkeys, measured by indirect immunofluorescence, were also comparable in serum and af.	1981	7021692
a simple apparatus for large-scale production of plasmodium falciparum in vitro.		1981	7021790
evaluation of in-vitro-cultured plasmodium falciparum as antigen for malaria serology.	a handicap in developing seroepidemiology for malaria in india is the lack of homologous antigens. aotus monkeys, in which human plasmodia can be propagated, are not easily available in india. other alternatives include the use of infected human blood or the in-vitro culture of plasmodium falciparum, using the candle-jar technique of trager & jensen (1976). with certain modifications, the in-vitro culture technique has been established at the st john's medical college (sjmc) to obtain a regular ...	1981	7021870
the distribution of plasmodium falciparum in the peripheral blood and bone marrow of gambian children.		1981	7022784
chloroquine-resistant falciparum malaria from east africa.		1981	7022785
characterization of antigens from erythrocytic stages of plasmodium falciparum reacting with human immune sera.		1981	7022789
a simplified culture technique for plasmodium falciparum.		1981	7023399
a simple automated device for the maintenance of plasmodium falciparum in suspension culture.		1981	7023400
a comparison of static thin layer and suspension cultures for the maintenance in vitro of plasmodium falciparum.		1981	7023403
entry of malaria parasites into resealed ghosts of human and simian erythrocytes.	whereas "white' ghosts, conventionally prepared by hypotonic lysis and washing are resistant to invasion by malaria parasites, cells lysed at high haematocrit and resealed without separating the membranes from the cytosol are invaded with high efficiency (not less than one-third the frequency of intact cells). similar results were obtained with human and monkey cells, exposed respectively to plasmodium falciparum and p. knowlesi. microspectrophotometry of individual cells was used to ascertain t ...	1981	7023533
[2 cases of disseminated intravascular coagulation in p. falciparum malaria: clinical and therapeutic considerations].		1981	7023803
hypoxanthine transport in normal and malaria-infected erythrocytes.		1981	7024014
[think of malaria!].	a drug addict, treated because of a lung abscess, was found to have plasmodium vivax malaria and was treated accordingly. the second patient acquired falciparum malaria inspite of chloroquine prophylaxis and was admitted with a thrombocytopenia of 24 000 mm3. after chloroquine treatment the platelet count became normal. thrombocytopenia is usually present in falciparum malaria. complications and their treatment are discussed.	1981	7024070
inhibition of in vitro growth of plasmodium falciparum by immune serum from monkeys.	three owl monkeys that had been immunized against the camp strain of plasmodium falciparum by infection were treated with chloroquine and rechallenged with parasites. immune serum caused a dose-dependent, time-dependent inhibition of in vitro parasite growth. heat-inactivation eliminated nonspecific inhibition by normal monkey serum without diminishing immune inhibition. purified igg from immune serum inhibited parasite growth. serum taken immediately before the second challenge did not inhibit ...	1981	7024432
plasmodium falciparum resistant to fansidar.		1981	7024758
[use of chemotherapeutic preparations in regions with a spread of chloroquine-resistant tropical malaria].		1981	7024770
[hemostatic system disorders in malaria (a review of the literature)].		1981	7024773
[search for new antimalarial compounds (a review of patents)].		1981	7024774
concurrent inhibition by tunicamycin of glycosylation and parasitemia in malarial parasites (plasmodium falciparum) cultured in human erythrocytes.	tunicamycin (0.1--10 microgram/ml) incubated 96 h with human erythrocytes infected with malarial parasites significantly decreased parasitemia compared to controls. the antimalarial effect of tunicamycin was dose-related and paralleled its inhibition of the incorporation of radiolabeled glucosamine into parasite-derived membrane macromolecules. tunicamycin had no significant effect on isoleucine incorporation into parasite-derived macromolecules. these results suggest that tunicamycin may act by ...	1981	7025026
2-deoxyglucose: inhibition of parasitemia and of glucosamine incorporation into glycosylated macromolecules, in malarial parasites (plasmodium falciparum).	cultured malarial parasites were incubated with various doses of 2-deoxyglucose (0.5, 1.0, 5.0, and 10.0 mm) for 48 h. effects of 2-deoxyglucose on parasite survival were determined by counting the number of parasites/10,000 erythrocytes. effects of 2-deoxyglucose on synthesis of membrane proteins and glycosylated macromolecules were monitored by metabolic labelling using radioactive isoleucine and glucosamine, respectively. 2-deoxyglucose significantly reduced parasite survival and synthesis of ...	1981	7025027
[treatment of malaria (p. falciparum) with clindamycin].		1981	7025170
syndrome of inappropriate antidiuretic hormone (siadh) in measles and malaria infections.	sodium concentrations and osmolalities of simultaneously obtained venous blood and urine have been determined in a total of 61 children consisting of 18 children with malaria infection, 18 children with measles infection and 25 age- and sex-matched controls. the mean plasma osmolalities in the malaria and measles groups were significantly lower than in the control group (p less than 0.01). there was no difference between the measles and malaria groups either in their plasma osmolality (p greater ...	1981	7025395
chemotherapy of malaria.	the chemotherapy of malaria is incomplete without taking into consideration the widespread presence of chloroquine resistant falciparum malaria necessitating the use of a combination of antimalarials in order to effect a radical cure. fortunately, there has been no evidence of chloroquine resistance thus far with p vivax and p malariae. chloroquine resistant falciparum strains are present in about 85% of patients suffering from falciparum malaria but fortunately over 90 percent of the resistant ...	1981	7025744
[drug resistance to malaria. apropos of 17 cases in 2 years].	17 cases of resistant p. falciparum malaria are reported. they were observed in different populations: on one hand 9 cambodian children refugees in france, on the other hand 8 french adults consulting after travels in south america or in south-east asia. different resistance levels to amino-4-quinolines and to a combination of sulfadoxine and pyrimethamine were found: they were more often from the ri type but sometimes from the riii type also. 6 patients were treated with mefloquine. its activit ...	1981	7026070
falciparum malaria among military recruits: emphasis on early diagnosis and treatment.		1981	7026230
cerebral malaria: a diagnostic dilemma. a case report.		1981	7026363
continuous in vitro cultivation of plasmodium falciparum: replacement of human serum by calf serum and proteose peptone.		1981	7026430
parasite-derived mitogenic activity for human t cells in plasmodium falciparum continuous cultures.	supernatants from plasmodium falciparum continuous cultures exhibited mitogenic activity against human blood lymphocytes from unsensitized donors. this effect, which was not observed with supernatants from control cultures grown in the absence of the parasites, was dependent upon (i) the concentration of supernatant added to the lymphocyte cultures and (ii) the parasite concentration in the p. falciparum continuous cultures. t cells were the predominant target cells of this mitogenic activity. w ...	1981	7026445
inhibitory effects of erythrocyte membrane proteins on the in vitro invasion of the human malarial parasite (plasmodium falciparum) into its host cell.	the intracellular development of the erythrocytic stage of the malarial parasite (merozoite) is initiated by the attachment of the parasite to the erythrocyte surface. this paper describes an assay system to investigate plasmodium falciparum merozoite entry into the host cell and reports on three observations regarding this interaction. (a) merozoites do not invade human erythrocytes treated with either trypsin or neuraminidase, and both enzymes partially cleave glycophorin a, the major erythroc ...	1981	7026577
in vitro studies of malarial parasites using resealed ghosts of human erythrocytes.	a method has been determined for the preparation of resealed erythrocyte ghosts that are suitable for in vitro studies of plasmodium falciparum. the resealed ghosts have been characterized with respect to their shape, size, stability in culture and their hemoglobin and atp contents. light and electron microscopy has revealed that parasites are able to develop normally in resealed ghosts. even resealed ghosts containing very reduced levels of the normal erythrocyte cytoplasmic constituents are ca ...	1981	7027270