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sero-epidemiology of amoebiasis in peninsular malaysia. 19826284074
seroconversion to entamoeba histolytica among short-term travelers to mexico.paired serum samples were evaluated for the presence of antibodies to entamoeba histolytica in 326 healthy college students from the united states who lived in guadalajara, mexico, for an average of four weeks. one hundred eighty of these students had an enteric illness develop, but no stool test results were positive for ameba. an indirect hemagglutination assay was the serologic method used, and no seroconversion was demonstrates. this finding confirms the belief that amebiasis is a rare cause ...19826284082
a preliminary study on the pathogenicity of axenically grown entamoeba histolytica for the golden hamster. 19826284104
purification and properties of nadp-linked, alcohol dehydrogenase from entamoeba histolytica.an nadp-linked, alcohol dehydrogenase from entamoeba histolytica was purified to apparent homogeneity by blue sepharose affinity chromatography. molecular weights of 130,000 and 30,000 were estimated by gel filtration and by sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, suggesting that the enzyme is a tetramer. the enzyme exhibited more than 20-fold selectivity for nadp(h) over nad(h). although the purified enzyme acts on both primary and secondary alcohols, higher act ...19826284905
mouse ige response to an allergen from entamoeba histolytica.attempts were made to demonstrate the presence of allergenic components in an extract of axenically cultured entamoeba histolytica (hk 9). various strains of mice were immunized i.p. with a low dose of the extract mixed with al(oh)3 and boosted with the same mixture 4 wk later. a high titer of ige antibody response was demonstrated in balb/c, dba/2 (h-2d), cba (h-2k), and swm strains but not in c3h/he (h-2k) strain. the extract was fractionated by gel filtration with sephadex g-200 and then by d ...19826284906
the epidemiology of entamoeba histolytica in a rural and an urban area of mexico. a pilot survey ii.stocks of intestinal amoebae grown in monoxenic culture, were compared against each other and against those previously reported from mexico city. these were isolated from subjects in san cristobal de las casas, chiapas (rural area) and hospital patients in merida, yucatan (urban area). electrophoretic patterns of the four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm), l-malate: nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk) demonstrated the pr ...19826285556
comparison of indirect fluorescent-antibody amoebic serology with counterimmunoelectrophoresis and indirect hemagglutination amoebic serologies.patients ranged from those with no prior diagnosis of or suspected exposure to entamoeba histolytica to those with proven amoebic liver abscesses (extraintestinal disease). a comparison of serologies from patients with proven and suspected amoebiasis or possible past exposure revealed good correlation between the indirect fluorescent antibody (ifa) procedure and the other methods used, counterimmunoelectrophoresis and indirect hemagglutination. titers from patients with proven extraintestinal am ...19826279694
amoebiasis: a review. 19826279839
importation of pathogenic entamoeba histolytica. 19826277421
the collagenase of entamoeba histolytica.the present work was designed to investigate the capacity of trophozoites of entamoeba histolytica to adhere to and digest human collagen types i and iii in vitro. the time-course of binding of ameba to both human collagen types i and iii was similar. however, the kinetics of detachment were different for each collagen type. trophozoites of e. histolytica cultured on heat-reconstituted type i collagen gels produced a well-defined area of lysis. quantitative studies using 14c-labeled collagen rev ...19826274995
a study of intestinal protozoa including non-pathogenic entamoeba histolytica from patients in a group of mental hospitals.twenty-five per cent of 174 patients residing in three mental hospitals were found to be infected with protozoa. seven per cent of the patients were infected with entameba histolytica. isoenzyme characterization of the e. histolytica grown in culture showed that there were no pathogenic zymodemes of this organism in any of the specimens tested. (am j public health 1982;72:178-180.)19826275721
studies on entamoeba histolytica infection in rural localities in banat. 19826303273
an ion-channel forming protein produced by entamoeba histolytica.we have identified a remarkable ion-channel forming material in virulent strains of entamoeba histolytica that may be responsible for many of the symptoms associated with amoebic dysentery. a polypeptide that we refer to as amoebapore is shed into the growth media and is also found within the amoeba in a high speed sedimentable fraction. amoebapore has the distinctive property of spontaneously incorporating into lipid bilayers, liposomes, and cells, leading to progressive and irreversible change ...19826329705
parasitic bowel disease: three pathogens important in primary care.a high index of suspicion and careful application of diagnostic methods are essential for accurate diagnosis of parasitic bowel diseases. the varied clinical spectrum of giardiasis, amebiasis, and strongyloidiasis emphasizes the need to consider these pathogens when patients present with gastrointestinal complaints. giardiasis should be suspected in patients, especially returned travelers, with unexplained increase in stool frequency, particularly with bloating, flatulence, or vague systemic sym ...19826280160
characterization of a membrane pore-forming protein from entamoeba histolytica.we describe the partial purification and characterization of a pore-forming material (pem) from entamoeba histolytica. the formation of ion channels by pfm was examined in three systems. (a) pfm depolarizes j774 macrophages and mouse spleen lymphocytes as measured by [3h]tpp+ uptake. (b) pfm induces rapid monovalent cation flux across the membrane of phosphatidylcholine-cholesterol vesicles. (c) pfm confers a voltage-dependent conductance to artificial planar bilayers, which is resolved as a sum ...19826294211
the colitides.diverse signs may be seen on barium enema in the various forms of colitis. barium enema is only reasonably specific in the diagnosis of crohn's disease and ulcerative colitis once the other possible diseases have been excluded from consideration by biopsy, microscopy, culture, or therapeutic trial. in particular, campylobacter colitis has proved to be such a common entity that many patients originally thought to have had one attack of ulcerative colitis, ischemic colitis, or crohn's colitis may ...19826294727
isolated entamoeba histolytica actin does not inhibit dnase-i activity. 19826280688
in vitro amoebicidal activity of immune cells.the amoebicidal activity of peripheral blood lymphocytes and spleen and peritoneal cells from hamsters vaccinated against or protected from hepatic amoebiasis and from those with hepatic amoebiasis was investigated. peripheral blood lymphocytes and peritoneal and spleen cells from vaccinated or protected animals can kill trophozoites of entamoeba histolytica in vitro. in contrast, spleen cells from infected hamsters showed no significant cytotoxic effect on the parasite. these data suggest that ...19826281189
attachment and ingestion of bacteria by trophozoites of entamoeba histolytica.entamoeba histolytica trophozoites were found to be very selective in their interactions with bacteria. two principal mechanisms were shown to be responsible for these interactions. certain bacteria, such as a number of escherichia coli and serratia marcescens strains which are known to contain mannose-binding components on their cell surface, bound to mannose receptors on the amoeba membrane. this attachment was markedly inhibited by alpha-methylmannoside (0.5%), especially when the incubations ...19826281192
the presentation of amoebiasis. 19826281593
virulence, haemolysis pattern and haemophagocytosis index of entamoeba histolytica. 19826295930
galactose-1-phosphate uridylyltransferase from entamoeba histolytica. 19826296622
6-phosphofructokinase (pyrophosphate) from entamoeba histolytica. 19826296625
immunology of entamoeba histolytica in human and animal hosts.although entamoeba histolytica induces humoral and cellular immune responses in both human and animal hosts, there is no indication of postinfection immunity in humans; in contrast, several other mammals are protected by prior infection or immunization. the exacerbation of the disease by immunosuppression suggests a protective function of still-unknown defense mechanisms. specific local and circulating antibodies are produced regularly during invasive amebiasis. although serum antibodies, togeth ...19826296962
a review of the parasite cellular mechanisms involved in the pathogenesis of amebiasis.amebiasis, a significant cause of morbidity and mortality on a global scale, is caused by invasion of the colonic mucosa by the cytolytic protozoan parasite entamoeba histolytica. a review of the investigations into the mechanisms by which the parasite causes invasive disease is presented, including the relevant history, epidemiology, and pathology of amebiasis, the biology of the parasite, in vivo and in vitro studies of the pathogenesis of disease, the host immune responses, and possibilities ...19826296963
amebiasis: host-pathogen biology.invasive amebiasis caused by entamoeba histolytica, and particularly amebic liver abscess, is a major public health problem in mexico and some other countries because of the high incidence and mortality of this disease. this paper first discusses the pathogenic effect of e. histolytica and the defensive response of the host and then reports studies concerning the experimental induction of protective immunity to amebic infection. the pathogenic effect of e. histolytica is probably initiated by a ...19826296964
a radioimmunoprecipitation polyethylene glycol assay for circulating entamoeba histolytica antigens. 19826298310
studies of organotin-schiff base complexes as new potential amebicidal agents. 19826298434
[clinico-parasitologic basic diagnosis of extraintestinal amebiasis and evaluative comparison of different methods in the diagnosis and course of amebic liver abscess]. 19826285626
diagnosis of amebic vaginitis from cervicovaginal smears. 19826285657
[amebiasis].infections with entamoeba histolytica do not necessarily cause disease in those infected. the parasite may act as commensal (cysts living in the bowel) or it may cause a broad spectrum of clinical illness. some of the factors causing overt disease are poorly understood. an acute amebic dysentery is accompanied by bloody stools, abdominal pain and indigestion. the most important extraintestinal complication of an amebic infection is a liver abscess causing severe pain, fever, nausea and vomiting. ...19826286439
adhesion of entamoeba histolytica trophozoites to human erythrocytes.to understand the mechanism of entamoeba histolytica adhesion, we characterized the binding of trophozoites to human erythrocytes (rbc) in suspension by measuring the kinetics of amoeba-rbc complex formation. adhesion was very efficient, since most of the amoebae were complexed with rbc after only 5 min at 37 degrees c in mixtures containing 10(4) amoebae and 10(6) rbc per ml; the adhesion rate depended on amoeba and rbc concentrations, but not on the a, b, and o human blood groups, and was maxi ...19826286491
effect of ion channel inhibitors on the cytopathogenicity of entamoeba histolytica.entamoeba histolytica (axenic strain hm1-imss), a cytolytic enteric pathogen, kills target chinese hamster ovary cells in two discrete steps: (1) carbohydrate-specific adherence of amoebae to target cells, followed by (2) cytolysis of adherent target cells. both steps require intact amoebic microfilament function. the effects of the fast na+ channel blocker tetrodotoxin and the slow na+-ca++ channel blockers verapamil (10(-5) m) and bepridil (10(-5) m) on amoebic killing were evaluated. verapami ...19826286794
indirect hemagglutination antibody titers for entamoeba histolytica in dried filter paper blood and sera.serum and elutates from dried filter paper bloods from 404 patients with and without amebic liver abscess (ala) were tested for entamoeba histolytica antibodies by the indirect hemagglutination test. eighty-nine percent of the sera from patients with ala were positive (titers greater than or equal to 1 : 128) and 94% of those without ala negative. fifty-five percent of the filter paper bloods from ala patients were positive. overall agreement was 80% for positives and negatives and the correlati ...19826287648
preliminary observations using a multi-layer elisa method for the detection of entamoeba histolytica trophozoite antigens in stool samples.a method for detecting entamoeba histolytica trophozoite antigens in aqueous solution is described. this involves coating plastic microtitre plates with a 'catching antibody', specific rabbit anti-e. histolytica antibody (srae). after adding the test material the presence of antigen is determined using two additional heterologous antibody layers, one of 'developing antibody', in this case human anti-e. histolytica immunoglobulin (hae), which is followed by a final layer of peroxidase conjugated ...19826287685
a zymodeme study of entamoeba histolytica in a group of south african schoolchildren.using a biphasic culture medium, stocks of intestinal amoebae were isolated from a group of children attending school in durban, south africa. these were compared with stocks collected in other areas of the world already characterized using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm) l-malate: nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk). 33% of 94 samples grew entamoeba histolytica, only one of which g ...19826287686
ultrastructure of entamoeba histolytica. a 96 hours old culture of an autochthonous strain. 19826287965
virulence of entamoeba histolytica strains from bombay (india) to rats.the results of intracaecal inoculation experiments on young albino rats with different strains of e. histolytica isolated in cultures from patients with different varieties of clinical amoebiasis, such as acute amoebic dysentery, amoebic liver abscess and chronic amoebic colitis, as well as from asymptomatic infections, have been presented and discussed. caecal scoring was performed with regard to the condition of the wall of the caecum and the contents of its lumen. successful infection with vi ...19826288796
analog inhibitors for the pyrophosphate-dependent phosphofructokinase of entamoeba histolytica and their effect on culture growth. 19826288907
macrophage requirement for host defence against experimental hepatic amoebiasis in the hamster.the role of macrophages in hepatic amoebiasis in hamsters has been investigated by means of their treatment with bacille calmette-guérin (bcg) for activation, and with silica for elimination of these cells. silica-treated animals inoculated intrahepatically with 1 x 10(5) trophozoites of entamoeba histolytica developed amoebic abscesses in the liver and more metastases to other organs than control animals, and this effect was silica-dose-dependent. in contrast, bcg-treated animals developed sign ...19826289225
amebiasis: nutritional implications.studies on the role of nutrition in amebiasis in humans and experimental animals are meager. some reports suggest that malnutrition of the host increases the incidence of infection and potentiates the severity of the disease. others suggest that malnutrition protects the host against invasion. a few reports indicate that dietary regimens can alleviate symptoms and even eradicate the parasite. others doubt a correlation between diet and rate of infection or disease manifestations. the problem is ...19826289405
progress in amebiasis. 19826289484
immunosuppression and experimental amoebiasis in guinea-pigs. 19826289762
effect of complement depletion on hepatic amoebiasis in hamsters. 19826290120
a solid-phase sandwich radioimmunoassay for entamoeba histolytica proteins and the detection of circulating antigens in amoebiasis.a sensitive and specific immunochemical assay for entamoeba histolytica antigens would be a valuable tool for clinical diagnosis, to study the sequelae of amoebiasis, and to screen for the expression of amoebic proteins in recombinant bacterial clones. the major impediment toward developing such an assay is the cross-reactivity of anti-e. histolytica antisera with a wide range of mammalian serum proteins. a rabbit anti-e. histolytica antiserum was repeatedly passed over three bovine serum protei ...19826290308
can we diagnose amebiasis? 19826290310
entamoeba histolytica cytotoxin: purification, characterization, strain virulence, and protease activity.a heat-labile cytotoxin was isolated from virulent strains of axenically cultivated entamoeba histolytica. strains of e. histolytica representing a spectrum of virulence as determined in animal and in vitro models of disease were examined for cytotoxic activity. extracts of virulent strain hm1 possessed marked cytotoxic activity, those of moderately virulent strain 200 showed intermediate activity, and those of avirulent strains 303 and rahman showed no activity. the cytotoxin was partially puri ...19826290574
a new liquid medium for xenic cultivation of entamoeba histolytica and other lumen-dwelling protozoa. 19826290631
similarities of lipid metabolism in mammalian and protozoan cells: an evolutionary hypothesis for the prevalence of atheroma. 19826290864
subcellular distribution and stability of the major hemolytic activity of entamoeba histolytica trophozoites.since the hemolytic activity of extracts from entamoeba histolytica trophozoites previously described by us might determine at least partially the necrotic lesions of amebiasis, we have continued its characterization in vitro. using rat erythrocytes as target cells, we have found that cytolysis by e. histolytica trophozoite extracts was (1) dose dependent, (2) localized mainly in a vesicular fraction whose absolute and specific activities were respectively 1.9 and 4.0 times higher than those of ...19826291272
on culture of entamoeba histolytica in plastic tissue culture flasks. 19826291273
natural transmission of entamoeba histolytica from mother cynomolgus monkeys (macaca fascicularis) to their newborn infants under indoor rearing conditions.natural transmission of entamoeba histolytica from infected mother cynomolgus monkeys (macaca fascicularis) to their newborn infants was investigated by the stool examination under indoor individually-caged conditions. every infant became positive for e. histolytica between 5 and 10 weeks after birth. although some infants were demonstrated to harbor the trophozoites, no overt amebic diarrhea occurred in any of the infants throughout the period of this study.19826291957
amoebiasis. 19826292416
cross-reaction of antiserum against enterobacterial common antigen with entamoeba histolytica hk-9 extracts. 19826295298
[ninth seminar on amebiasis. 9-11 november 1981]. 19826295299
the effects of prostaglandins on the secretion resulting from entamoeba histolytica "enterotoxin". 19826295300
adherence and ingestion of bacteria by trophozoites of entamoeba histolytica. 19826295301
separation of adherence, cytolytic, and phagocytic events in the cytopathogenic mechanisms of entamoeba histolytica. 19826295302
[isolation of clones of e. histolytica deficient in adhesion to human erythrocytes]. 19826295303
[clones of e. histolytica deficient in phagocytosis present a deficiency in virulence]. 19826295304
[morphology of trophozoites of entamoeba histolytica on natural substrates. study using scanning electron microscopy]. 19826295305
[interactions between lectin and receptor mediate the adhesion of e. histolytica to epithelial cells. relation of adhesion to the virulence of the strains]. 19826295306
[detection of early cellular damage produced by entamoeba histolytica in epithelia]. 19826295307
[receptors participating in the adhesion of e. histolytica to human erythrocytes]. 19826295308
virulence of entamoeba histolytica upon continuous axenic cultivation. 19826295309
[presence of collagenolytic activity in trophozoites of entamoeba histolytica]. 19826295310
phagosomal membranes from entamoeba histolytica: isolation and biochemical properties. 19826295311
[cytotoxic activity of entamoeba histolytica on lymphoid cells of mouse spleen]. 19826295312
high salt sds-dep technic for isolation of "intact" rrna from entamoeba histolytica. 19826295313
[interaction between entamoeba histolytica and the cecal epithelium of the guinea pig. quantitative study]. 19826295314
activation of lymphocytes from healthy donors and patients with amebiasis by extracts of entamoeba histolytica. 19826295315
antigen fraction from entamoeba histolytica strain hk9 for use in elisa. 19826295316
[excretion of anti-ameba specific antibodies of class iga in bile from rats immunized with trophozoites of entamoeba histolytica cultivated in axenic media]. 19826295317
the nitroblue tetrazolium (nbt) reduction of entamoeba histolytica during endocytosis of e. coli and homologous antibodies. 19826295318
[effect of cycloheximide on the inhibition of chemotaxis in human monocytes caused by products of e. histolytica]. 19826295319
[partial purification of several surface antigens of e. histolytica. investigation of the biological activity with respect to sera from patients with amebic liver abscess]. 19826295320
rna depolymerase in entamoeba histolytica: soluble vs ribosomal. 19826295321
[monoclonal antibodies against entamoeba histolytica]. 19826295322
[identification of circulating amebic antigen in man by immunoenzymatic analysis. i. development of the technic]. 19826295323
[detection of amebic antigen by the elisa method in sera of patients with hepatic abscess]. 19826295324
[long term detection of anti-amebic antibodies by counterimmunoelectrophoresis and indirect hemagglutination]. 19826295325
[localization of actin in trophozoites of entamoeba histolytica (hmi)]. 19826295326
in vitro activity of certain quassinoid anti-tumor agents against entamoeba histolytica. 19826295327
[immunochemistry of a lipopeptidophosphoglycan extracted from trophozoites of entamoeba histolytica strain hk-9 cultivated in axenic media, using the phenol-water method]. 19826295328
[localization of a lipopeptidophosphoglycan extracted by phenol-water from trophozoites of the hk-9 strain of entamoeba histolytica]. 19826295329
specificity of iron requirements of entamoeba histolytica in vitro. 19826295330
protein biosynthesis by entamoeba histolytica in culture. 19826295331
[clinical correlation of zymodemes of e. histolytica]. 19826295332
observations on zymodeme studies of entamoeba histolytica in durban, south africa. 19826295333
a review of isoenzyme characterization of entamoeba histolytica with particular reference to pathogenic and non-pathogenic stocks isolated in mexico. 19826295334
membrane dynamics of entamoeba histolytica and giardia lamblia studied with reflection contrast microscopy and the carbon immunoassay. 19826295335
bruceantin, a potent amoebicide from a plant, brucea antidysenterica.bruceantin, purified from an ethiopian plant used to treat dysentery, killed entamoeba histolytica in vitro (ic50 [the concentration of drug which decreased the number of colonies to half that of controls] = 0.018 microgram/ml). six related quassinoids of 17 tested were also amoebicidal. no relationship between quassinoid structure and amoebicidal activity was apparent.19826100431
an epidemiological investigation of health risks related to solid waste salvage and recycling in an egyptian community.this is the first report of an epidemiological cross-sectional study of solid waste scavengers, called zabaline, in egypt. the zabaline communities provide an important solid waste collection service throughout egypt's urban sector. their economic viability depends entirely on salvaging solid waste for recycling. intestinal parasites were common among the zabaline examined; 48% were found with one or more protozoan or helminth infections. either schistosoma haematobium or s. mansoni or both infe ...19826983749
in vitro lymphocyte stimulation in patients with lepromatous and borderline tuberculoid leprosy. the effect of dapsone treatment on the response to mycobacterium leprae antigens, tuberculin purified protein derivative and non-mycobacterial stimulants.lymphocytes from peripheral blood were isolated from leprosy patients and healthy contacts (hc) of leprosy patients and stimulated in vitro with: mycobacterium leprae and a m. leprae cell wall antigen, mlw 1; tuberculin purified protein derivative (ppd); antigens prepared from candida albicans, entamoeba histolytica, leishmania aethiopica, and parotitis virus; the non-specific mitogens phytohemagglutinin (pha) and concanavalin a (con-a). lymphocytes from patients with untreated lepromatous lepro ...19826763003
cytologic detection of parasitic disorders.parasitic diseases are common in developing countries; yet they are infrequently diagnosed in cytologic material. six cases are described where routine cytologic examination identified the parasites and suggested the diagnosis. there were three cases of entamoeba histolytica infection of the female genital tract and one each of hydatid cyst of the brain, strongyloides stercoralis infection of the lung and filarial infection of the urinary bladder. to our knowledge, cytologic diagnosis of hydatid ...19827048830
vibrio parahaemolyticus: a major cause of travelers' diarrhea in bangkok.travelers' diarrhea in bangkok, thailand was investigated in a group of 146 hotel guests who had diarrhea during their stay in the city. the identified pathogenic organisms were vibrio parahaemolyticus in 45 (31%), vibrio cholerae in 1, nonagglutinable vibrio in 1, and entamoeba histolytica in 3. identification of enterotoxigenic escherichia coli was not attempted. the study suggests that vibrio parahaemolyticus is a major cause of travelers' diarrhea in bangkok.19827058975
amebic abscess of the liver: surgical aspects.during the past five years, ten documented cases of hepatic amebic abscess have required surgical intervention at olive view hospital, van nuys, california. three of the patients underwent laparotomy when an hepatic abscess ruptured into the peritoneal cavity, and two required surgical intervention when an abscess invaded adjacent organs. another abscess continued to enlarge despite metronidazole therapy and still another became superinfected with bacteria. in the remaining three patients, surgi ...19827064468
[diarrhea caused by parasites].the role of parasites as etiologic agents in diarrhea is emphasized. in countries of western europe, entamoeba histolytica and giardia lamblia are the most prevalent species, although some other 20 protozoans and helminths may lead to diarrheic disturbances. parasitology, pathogenesis, clinical signs, diagnosis and therapy are briefly outlined. the need for competence on the part of the personnel responsible for laboratory diagnosis is emphasized; also, newly developed immunodiagnostic methods m ...19827079687
intestinal parasites in asymptomatic adult southeast asian immigrants.all previous studies of intestinal parasites in southeast asian refugees have included immigrants with and without symptoms. in order to determine the prevalence of intestinal parasites in asymptomatic southeast asians, 226 adult refugees were studied who had no symptoms or signs suggestive of intestinal parasitism and who submitted one to three stools for examination. fifty-four percent of these asymptomatic adults were infected with one or more potential pathogens, 22 percent with ascaris, 20 ...19827108458
attempts to immunize golden hamsters against entamoeba histolytica. 19827125761
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