Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [recurrent vaginitis]. | vaginitis is one of the most frequent infections of the female genital system and, in the united states, represents the main reason for gynecological consultation. candida albicans, trichomonas vaginalis and gardnerella vaginalis are responsible for 90% of cases of vaginal phlogosis. it has been calculated that a percentage varying between 5% and 40% of the female population will suffer, at least once in their lifetime, from an episode of vaginal candidosis. the re-appearance of symptoms after s ... | 1989 | 2484480 |
| [etiology of cervicovaginal infection in pregnant and non-pregnant patients]. | a prospective study to evaluate the prevalence of specific microorganisms in pregnant and nonpregnant women with cervicovaginitis attended at the instituto nacional de perinatología, was done; candida sp, gardnerella vaginalis, replasma urealitycum, chlamydia trachomatis, group b streptococcus, trichomonas vaginalis, neisseria gonorrhoeae were investigated. two hundred thirty four patients were studied, 105/234 (44.9%) pregnant and 129/234 (55.1%) nonpregnant women, with a mean age of 26.5 years ... | 1989 | 2486979 |
| [trichomonas vaginalis infection in pregnant women and newborn infants]. | we studied 149 pregnant women at delivery and 113 newborns in order to determine the frequency of t vaginalis infection. neutral red stain, dark field microscopy and culture methods were used with comparable yields. the age of women ranged from 12 to 43 years. a 27.5% infection rate was detected in mothers but all newborns were free from infection. infection rates in relation to age among mothers confirmed previously reports. | 1989 | 2487951 |
| prevalence of chlamydia trachomatis infection in a population of asymptomatic women in a screening program for cervical cancer. | the prevalence of chlamydia trachomatis infection in a population of women with no symptoms of sexually transmitted disease was investigated. these women, aged 35-55 years, participated in a screening program for cervical cancer. with the use of a direct immunofluorescence method, 109 out of 2,470 smears tested were positive for chlamydia trachomatis, indicating an overall prevalence of 4.4%. no changes in prevalence were found when five-year cohorts of this group were analyzed, indicating that ... | 1989 | 2498093 |
| sexually transmitted pathogens in acute pelvic inflammatory disease. | the prevalence of sexually transmitted pathogens in two groups of women was studied: 50 women with clinical diagnoses of acute pelvic inflammatory disease (pid) and 50 asymptomatic women attending a family planning clinic (fpc). genital specimens, collected by non-invasive procedures, were examined. endocervical neisseria gonorrhoeae was present in 62% of the pid group and 10% of the fpc group (p less than 0.0001). one-third of n. gonorrhoeae isolates were penicillinase-producing strains. chlamy ... | 1989 | 2506653 |
| vaginal microbial flora in women with and without vaginal discharge registered in general practice. | to compare the microbiological findings for patients with and without vaginal discharge, 29 general practitioners registered 361 women with and 229 women without complaints of vaginal discharge in a multi-practice study in the county of aarhus, denmark. in five of the patients (1.4%) with vaginal discharge, neisseria gonorrhoeae was isolated compared with one (0.4%) in women without vaginal discharge. chlamydia trachomatis was isolated in 30 (8.3%) and nine (3.9) respectively, candida in 113 (31 ... | 1989 | 2509146 |
| bacterial vaginosis: prevalence in outpatients, association with some micro-organisms and laboratory indices. | seven hundred and ninety three women were investigated, aged between 16 and 78 years, to evaluate the prevalence of bacterial vaginosis (bv) and some associated micro-organisms, and to discuss the significance of laboratory indices correlated to this pathology. bv was diagnosed on the basis of four distinct criteria: a positive result of the test for amines with 10% koh (odour-test), the presence of clue cells on fresh microscopic examination, a ph greater than 4.5 and direct gram stain positive ... | 1989 | 2515148 |
| [macrophage activation and lymphokine production in the mouse by lymphocytes sensitized against trichomonas vaginalis]. | in this study we have examined whether resident peritoneal macrophages could be activated in vitro by immune t lymphocytes obtained from mice which were immunized with live trichomonas vaginalis (t. vaginalis). t lymphocytes obtained from mice previously sensitized against t. vaginalis, showed a significant proliferative response when cultured in vitro in the presence of t. vaginalis. resident peritoneal macrophages obtained from untreated syngenic balb/c mice revealed an increasing cytotoxic ac ... | 1989 | 2518763 |
| detection of sexually transmitted diseases by urethral cytology, the ignored male counterpart of cervical cytology. | the detection of sexually transmitted diseases by urethral cytology was investigated in 270 men examined by urethral swabbing smears. each sample was used to prepare a wet mount smear and smears for staining by the papanicolaou, gram and methylene blue techniques. a fifth smear was used for direct staining with fluorescein-conjugated monoclonal antibodies for the detection of chlamydia trachomatis. the smears were examined for cytoplasmic and nuclear changes as well as for pathogenic organisms a ... | 1989 | 2536992 |
| tritrichomonas foetus: purification and characterization of hydrogenosomal atp:amp phosphotransferase (adenylate kinase). | the hydrogenosomal enzyme atp:amp phosphotransferase (adenylate kinase) (ec 2.7.4.3) was purified to apparent homogeneity from the bovine parasite tritrichomonas foetus. a fraction enriched for hydrogenosomes was obtained from cell homogenates which had been subjected to differential and isopycnic centrifugation. adenylate kinase was solubilized in 50 mm tris-hcl, ph 7.3, containing 0.8% triton x-100, and purified by sequential affi-gel blue affinity chromatography and high-performance liquid ch ... | 1989 | 2546792 |
| cytopathology of sexually transmitted disease. | although other methods exist for routine identification of most sexually transmitted diseases, the pathologist can often recognize the etiologic agents or their cytologic effects on papanicolaou (pap) smear. useful clinical information requires both the ability to recognize these effects and knowledge of their limitations. pap smear changes characteristic of trichomonas vaginalis, chlamydia trachomatis, herpes simplex virus, gardnerella vaginalis, candida sp., and human papillomavirus infections ... | 1989 | 2551568 |
| evidence of the non-infectivity of herpes simplex viral particles in trichomonas vaginalis. | fan et al., employing an indirect immunofluorescent antibody (ifa) technique, reported the presence of herpes simplex virus type 2 (hsv-2) in trichomonas vaginalis. however, using the same method, we found that the protozoa showed autofluorescence immediately after acetone fixation. in order to demonstrate the non-infectivity of hsv-2 in t. vaginalis, several other methods were performed in this study. trichomonads were experimentally incubated together with hsv-2 and examined after immunofluore ... | 1989 | 2558006 |
| presence of a fructose-2,6-bisphosphate-insensitive pyrophosphate: fructose-6-phosphate phosphotransferase in the anaerobic protozoa tritrichomonas foetus, trichomonas vaginalis and isotricha prostoma. | extracts of the anaerobic protozoa tritrichomonas foetus, trichomonas vaginalis and isotricha prostoma contained a high activity (0.5-1 mumol min-1 (mg protein)-1) of pyrophosphate:fructose-6-phosphate phosphotransferase (ppi-pfk), but no detectable atp: fructose-6-phosphate phosphotransferase. ppi-pfk from i. prostoma was purified close to homogeneity by adsorption on phospho-ultrogel and elution with fructose-1,6-bisphosphate, and subsequent anion-exchange chromatography. the enzyme had an mr ... | 1989 | 2558319 |
| acquisition and retention of viruses by trichomonas vaginalis. | recently described occurrence of virus-like particles (vlp) in some strains of trichomonas vaginalis suggests the possibility that the pathogenic significance of this organism may be broadened by its potential for viral transmission. inasmuch as neither the source nor the host range of the vlp are known, any hazard which they may present for man cannot be estimated. a model has been established for the study of acquisition of known human viruses by t vaginalis. tissue cultures were infected with ... | 1989 | 2559023 |
| a simple micropore system for experimental studies on trichomonad parasites. | a simple leak-free micropore chamber containing protozoan parasite species was implanted subcutaneously on the back of hamsters and evaluated for viability and multiplication of protozoan parasites. trophozoites of defined strains of entamoeba histolytica, giardia lamblia, trichomonas vaginalis, and tritrichomonas foetus were used; their survival and multiplication in the chambers formed the basis of evaluation. entamoeba histolytica and g. lamblia did not survive more than 6 hr and succumbed du ... | 1989 | 2559180 |
| rapid diagnosis of common sexually transmitted diseases in adolescents: a review. | sexually transmitted diseases (stds) represent a significant health problem for adolescents in the united states. as a result, clinicians from a number of disciplines, among them pediatrics and dermatology, may be called on to evaluate teenage patients with these infections. traditionally, the diagnosis of many stds has relied on isolation of the causative organisms in culture. while such procedures typically offer optimal diagnostic accuracy, they are often time consuming and expensive, and may ... | 1989 | 2559398 |
| [an ultracytochemical study of the hydrogenosomes of trichomonas vaginalis]. | the ultracytochemical study of cultural t. vaginalis has been carried out. the activity of magnesium-dependent atpase, transport (sodium-potassium-dependent) atpase and adenylate cyclase was detected in dense corpuscles, located on the membranes of hydrogenosomes having a rounded form. the authors suggest that the dense corpuscles are the zones of the active transport of ions through the membranes of hydrogenosomes. | 1989 | 2559564 |
| sexually transmitted diseases and chlamydia trachomatis in women consulting for contraception. | to study the frequency of genital infections in women consulting their family doctor for contraception, 248 women (median age 23 years) were examined for a range of genital microorganisms. the prevalence of clue cells, candida albicans and trichomonas vaginalis were 21.0%, 12.9% and 2.0%, respectively. neisseria gonorrhoeae was isolated in only one case, whereas chlamydia trachomatis was found in 6.3% of women. a specific clinical picture for an infection with c. trachomatis in women was not see ... | 1989 | 2560023 |
| effect of heat inactivation of sera on anti-trichomonas vaginalis igg elisa. | 1989 | 2584418 | |
| characterization of a thiol proteinase in giardia lamblia. | sonicated preparations of giardia lamblia hydrolyze a variety of protein substrates including human immunoglobulin. activity is increased by thiol-activating agents and inhibited by thiol proteinase inhibitors. about 55% of activity remains in the soluble fraction after high-speed centrifugation, and pretreatment with a nonionic detergent results in increased soluble activity. this suggests that the enzyme is membrane bound or associated with subcellular particles. activity elutes as a major pea ... | 1989 | 2584755 |
| cervical tuberculosis coexisting with trichomonas vaginalis. | 1989 | 2588932 | |
| [urethritis cause by round-form trichomonas in women]. | 1989 | 2589833 | |
| [urogenital trichomoniasis and vaginal mycosis in female offenders--a repeat study 20 years later]. | the prevalence of urogenital trichomoniasis and vaginal mycosis in 698 female prison inmates in bratislava was compared with the occurrence of the diseases recorded twenty years ago. the occurrence rate of urogenital trichomoniasis (40.97%) was only slightly reduced compared to the sixties (59.17%), yet it was still five times higher than in the current population. the occurrence rate of vaginal mycosis is much lower in delinquent women (2.3%) than in other women. trichomonas vaginalis was most ... | 1989 | 2590856 |
| suppression of humoral immune responses in mice by antigens derived from trichomonas vaginalis. | the occurrence of immunosuppression in mice inoculated with antigens prepared from trichomonas vaginalis was investigated in this study. one hundred and fourteen balb/c mice with half of each sex were divided into 19 groups and inoculated with different antigen preparations before or after sheep red blood cell (srbc) immunization. antigen preparations included live t. vaginalis (ltv), excretory and secretory products (esp), and freeze and thaw antigens (fta). the immunosuppressive effects of the ... | 1989 | 2594864 |
| endocervical chlamydial infection in women attending a sexually transmitted disease clinic in singapore. | two hundred women attending the sexually transmitted disease (std) clinic at middle road hospital were investigated. chlamydia trachomatis was isolated from 32% of women who were contacts of men with nongonococcal urethritis, 15% of contacts of gonococcal urethritis, 27% of contacts of unspecified std, and 13% of women without any history of std in their sex partners. overall, chlamydia trachomatis was isolated from 17% of 200 women, neisseria gonorrhoeae from 13% of 199 women, candida albicans ... | 1989 | 2609175 |
| comparative in vitro sensitivity to metronidazole of trichomonas vaginalis with isolates and clones. | isolates of trichomonas vaginalis from a group of thai women in khon kaen, thailand were studies for their in vitro sensitivity to metronidazole. forty-five isolates of t. vaginalis and further 226 laboratory clones of these isolates were examined for drug sensitivity under anaerobic conditions using the cplm (cysteine-peptone-liver infusion maltose) medium. the in vitro sensitivity of t. vaginalis to metronidazole, recorded as mlc values revealed highly variable sensitivity profiles greater tha ... | 1989 | 2609217 |
| [the diagnostic value of a culture study in detecting patients with trichomoniasis]. | the advantages of culture method for the diagnosis of trichomoniasis over microscopy of stained and native preparations are demonstrated. new liquid nutrient media for trichomonas cultivation reveal vaginal trichomonas in 99.48% of cases in medium, in 100% of cases in medium, and in 97.78% of cases. these media are universal and may be used at bacteriologic laboratories of treatment-and-prophylaxis institutions engaged in culture diagnosis of trichomoniasis. | 1989 | 2609750 |
| [synthesis of 2-aryl benzothiazoles and research on its antiparasitic activity]. | several 2-aryl benzothiazoles (with phenyl, naphthyl, thienyl and some oxygenated heterocycles) were synthesized. the anti-parasitic properties of these compounds were evaluated, in vitro and in vivo, against one nematode nippostrongylus brasiliensis and, in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. only two compounds exhibited, in vitro a low nematicidal activity. | 1989 | 2610470 |
| trichomonas vaginalis infection in a lesbian. | 1989 | 2613220 | |
| prevalence of common infective agents of vaginitis. | a total of 100 women with vaginitis and 50 age matched healthy women were studied for common infective agents of vaginitis. the vaginal secretions collected from these women were examined for trichomonas vaginalis, g. vaginalis, and candida species. the prevalence of infective agents in the patients was t. vaginalis 11 per cent. candida species 30 per cent and gardnerella vaginalis 31 per cent. in contrast, in the control group the prevalence was candida species 14 per cent and g. vaginalis 22 p ... | 1989 | 2614050 |
| study of candida and trichomonas vaginalis in leucorrhoea. | of 150 clinically suspected cases of leucorrhoea, 44 cases (29.33%) showed growth of candida and 30 cases (20%) of trichomonas, by wet preparation. among the candida species the most frequent isolate was c albicans (56.8%), followed by c tropicalis (20.4%), c krusei (11.4%), c stellatoidea (9.1%) and c parapsilosis (2.3%). the higher incidence was found among the pregnant women than among non-pregnant women. among pregnant women multipara in third trimester showed higher incidence of candida and ... | 1989 | 2621360 |
| glycogen medium, antitrichomonal drug activity in vaginal liquids. | the amount of glycogen in vaginal liquids decreases with trichomonas vaginalis and this is connected with t. vaginalis activity in specimens. a glycogen-hydrolysed casein medium ("glycogen medium") added to vaginal liquid is a valid maintenance medium for t. vaginalis and therefore enables a direct test for antitrichomonas drugs to be performed. | 1989 | 2637795 |
| evaluation and management of vaginitis. an update for primary care practitioners. | there are three major causes of vaginitis symptoms that primary care practitioners should be able to diagnose and treat expertly. recent investigations have shown that bacterial vaginosis, the most common cause of vaginitis symptoms in patients not seen in sexually transmitted disease clinics, has a multifactorial etiology: gardnerella vaginalis is universally present but is not, per se, the etiologic agent. diagnosis and treatment are based, therefore, on evidence of a disturbed bacterial ecolo ... | 1989 | 2645843 |
| epidemiology of sexually transmitted diseases in rape victims. | rape is a violent crime that is increasing rapidly in incidence. victims can include male and female adults and children. these victims may be at risk for the acquisition of many sexually transmitted conditions. the risk will vary with the sex and socioeconomic status of the assailant as well as with the nature of the sexual contact. infections with neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, herpes simplex virus, human papillomavirus, and treponema pallidum have all bee ... | 1989 | 2649960 |
| [epidemiologic aspects of trichomoniasis]. | 1989 | 2655345 | |
| the emergency department diagnosis of trichomonas vaginitis. | trichomonas vaginalis is a common cause of vaginitis in emergency department patient populations. while the diagnosis of this condition is usually on the basis of wet-mount microscope slide preparations, other diagnostic technology exists. we studied the accuracy of the traditional wet-mount technique as compared with detection using direct immunofluorescence and culture techniques in ed patients with the presenting complaint of vaginal discharge. of 157 patients evaluated, the wet-mount techniq ... | 1989 | 2655509 |
| differentiation of gardnerella vaginalis, candida albicans, and trichomonas vaginalis infections of the vagina. | this study evaluated the positive predictive values of factors associated with gardnerella vaginalis, candida albicans, and trichomonas vaginalis for diagnosing vaginitis in a community-based population. one hundred ninety-six women with and without vaginal complaints were evaluated for historical factors, physical examination findings, and office laboratory results that were potentially associated with each of the three vaginal organisms. extensive microbiological tests were performed to detect ... | 1989 | 2656903 |
| carbohydrate, energy and hydrogenosomal metabolism of tritrichomonas foetus and trichomonas vaginalis. | 1989 | 2657037 | |
| trichomonas vaginalis. | although not life-threatening, trichomoniasis is a major health problem, especially in women. it is transmitted sexually and has a very high incidence worldwide. we argue that trichomonas vaginalis is not a commensal. attempts should be made to identify this pathogen in all suspicious cases. although culturing remains the most sensitive method to detect t vaginalis, newer methodologies such as monoclonal antibody fluorescent and direct staining make it possible to diagnose an infection with high ... | 1989 | 2668827 |
| vaginitis/vaginosis. | vaginitis/vaginosis is an extremely common medical problem. most cases are caused by yeast (predominantly candida albicans), the protozoan trichomonas vaginalis, or a specific mixture of bacteria (bacterial vaginosis). the prevalence of each of these varies with the patient population. the clinical signs used in differentiating among these and the associated bacterial flora are shown in table 3. because vaginitis/vaginosis cannot be adequately diagnosed solely on the basis of symptoms or physica ... | 1989 | 2676321 |
| trichomonas vaginalis. | trichomonas vaginalis is one of the most common sexually transmitted diseases. the epidemiology, diagnosis, treatment, and concerns about transmission are discussed. | 1989 | 2687745 |
| [retrospective study of mycotic and other colpitis pathogens]. | we had 170 female patients with presenting symptoms of vaginal itching, dysuria, dyspareunia, burning and vaginal discharge. we performed 200 times vaginal microbial exploration on the said 170 patients, with the repetition of the procedure on 30 out of these 200 patients for the second time. we had a positive result for vulvovaginal candidosis on 60 occasions. these positive results for yeast infection can be related to the incidence of other microbial infections such as mycoplasma-, ureaplasma ... | 1989 | 2688322 |
| [use of native microscopic examination of the vaginal flora in the diagnosis of trichomoniasis]. | microscopic diagnosis of vaginal discharge established so far by means of microbial vaginal pictures (mvp) has proved to be insufficient. phase contrast microscopy has an irreplaceable value in diagnosing vaginal infections transmitted by sexual intercourse. it yields quick and exact diagnosis of the disease, thus providing the possibility to administer treatment addressly and immediately. in equivocal findings and relapses vaginal cultivation is indispensable. in a series of 600 examined women ... | 1989 | 2691022 |
| bacterial vaginosis. | bacterial vaginosis is a common, non-inflammatory infection of the vagina. it is characterised by the presence of a thin, homogenous, greyish-white discharge. the differential diagnosis includes infection with trichomonas vaginalis and candida albicans. a diagnosis of bacterial vaginosis may be made by the detection of three of the following: characteristic discharge, ph of 5 or greater, clue cells and a positive koh amine test. culture of vaginal discharge is not necessary to effect a diagnosis ... | 1989 | 2694618 |
| new derivatives of 5-nitroimidazole: synthesis and antiparasitic activity. | the synthesis and the antiparasitic evaluation of twelve new 5-nitroimidaole derivatives has been carried out. the most effective compounds were the less hydrophilic pyridinium and imidazolium salts (iv), (v) and (x), and above all the tetrahydropyridine derivatives (xii) and (xiii). | 1989 | 2701965 |
| evaluation of proposed cytomorphologic criteria for the diagnosis of chlamydia trachomatis in papanicolaou smears. | to evaluate the proposed cytomorphologic criteria for the cervical cytologic diagnosis of chlamydia trachomatis infection, a study was made of 171 endocervical smears. all cytomorphologic elements that could be ascribed to chlamydia trachomatis infection were correlated with the diagnostic confirmation of this microorganism by monoclonal antibody (mab) staining. the presence of chlamydia trachomatis was detected in 21 samples (12.28%) by mab staining. comparing the cytomorphologic results with t ... | 1989 | 2728785 |
| association of chlamydia trachomatis and mycoplasma hominis with intrauterine growth retardation and preterm delivery. the john hopkins study of cervicitis and adverse pregnancy outcome. | the authors conducted a prospective study of risk factors for intrauterine growth retardation (birth weight less than a standard (race- and sex-adjusted) 10th percentile for gestational age) and preterm birth (birth prior to 37 weeks gestation) in a high-risk, inner-city, obstetric population, with special interest in pathogens colonizing the maternal genital tract. a total of 801 women within 22 to 30 weeks of gestation were enrolled and interviewed. subjects were cultured for gardnerella vagin ... | 1989 | 2729260 |
| infection with trichomonas vaginalis in a black population. | trichomonas vaginalis infection (tvi) was found by examination of pap smears in 25% of 3,005 unselected urban black women being screened for cancer of the cervix and vagina. the incidence was 22% in the group under 29 years of age; 69% in those between 30 and 59; and 9% in those over 60. women who had had a hysterectomy had 16% lower incidence of tvi than did the controls. class 1 reports (atypia, metaplasia, hyperplasia) were obtained in 10% of the entire group. cytologic changes were present i ... | 1989 | 2746693 |
| diagnostic procedures in vaginitis. | ninety-seven vaginitis patients underwent a standardized direct method of examination. bacterial vaginosis was found in 38.1%, candida albicans in 24.7% and trichomonas vaginalis in 13.4%. low secretional ph values were never met in bacterial vaginosis and t. vaginalis cases. a fishy odor, present in bacterial vaginosis, was also found in almost half of the t. vaginalis cases. endocervical bacteriological cultures are probably insufficient with respect to the detection of c. albicans and gardner ... | 1989 | 2759324 |
| differences in biologic maturation, sexual behavior, and sexually transmitted disease between adolescents with and without cervical intraepithelial neoplasia. | because several cofactors may influence the development of cervical intraepithelial neoplasia (cin) in young women, we compared differences in behaviors (sexual activity, contraception, and cigarette use), sexually transmitted disease (std) infection rates (chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and herpes simplex virus), and cervical maturation (age of menarche and percentage of cervical ectopy) in groups of sexually active female adolescents with and without cin. ... | 1989 | 2769512 |
| clinical manifestations of vaginal trichomoniasis. | trichomonas vaginalis was detected by culture or wet-mount examination in 118 (15%) of 779 randomly selected women attending a sexually transmitted disease clinic. vaginal trichomoniasis was significantly associated with symptoms of yellow discharge, abnormal vaginal odor, and vulvar itching and with signs of colpitis macularis ("strawberry cervix"), purulent vaginal discharge, and vaginal and vulvar erythema. a logistic regression model was used to adjust for coinfections, as well as for demogr ... | 1989 | 2783346 |
| scanning electron microscopy of trichomonas vaginalis. | by scanning electron microscopy (sem), t. vaginalis surface appeared pleated with crater-like depressions. all the flagella appeared to start off in a single bundle from a shallow depression with a prominent rim. the side carrying the undulating membrane is extensively ruffled having a mulberry-like appearance to which flocculent material was frequently adherent. t. vaginalis nutrition appeared to proceed through pseudopodia-like extensions and phagocytosis by a constant deep invagination at the ... | 1989 | 2785150 |
| relatedness of structures of a major immunogen in trichomonas vaginalis isolates. | solubilization of live trichomonas vaginalis organisms with detergent caused the release of cysteine proteinases in the detergent extract which were inhibitable with n-alpha-p-tosyl-l-lysine chloromethyl ketone. the detergent extracts of all isolates tested possessed similar cysteine proteinase activities. these parasite proteinases rapidly degraded a prominent immunogen whose surface disposition undergoes phenotypic variation in some isolates. the relatedness of the forms of this immunogen amon ... | 1989 | 2785964 |
| [observation of the processes of phagocytosis and digestion of staphylococcus aureus by trichomonas vaginalis using a transmission electron microscope]. | trichomonas vaginalis was incubated with bacteria for 30 min. after centrifugation of staphylococcus aureus, incubation of t. vaginalis was continued for 40 min., 120 min. and 6 hrs. it was found that bacteria have been intensively phagocytated through whole surface of protozoan. phagosome with bacteria at different stages of degradation, up to defecation of undigested residues were observed. it was observed that some bacteria, apparently not susceptible to digestion, were immersed directly in c ... | 1989 | 2786283 |
| comparison of diamond's medium modified and kupferberg medium for detection of trichomonas vaginalis. | diamond's medium modified, the commercially available version of the klaas modification of diamond's medium, was compared to kupferberg medium and to direct wet-mount examination for detection of trichomonads in symptomatic patients. diamond's medium was found to be superior to both kupferberg medium (p less than 10(-4) and wet mount (p less than 10(-6]. | 1989 | 2787332 |
| secretory hydrolases of trichomonas vaginalis. | supernatants of three trichomonas vaginalis strains obtained from culture or after contact and cultivation with wish cells were assayed to characterize the nature of the factor/s responsible for the cytotoxic activity on wish cell monolayers. this factor, mainly secreted from the live, exponentially grown parasite, with a molecular weight higher than 10,000 daltons and heat-unstable, does not appear to be a protease. in its place, we demonstrated that the presence of some hydrolases (particularl ... | 1989 | 2787467 |
| survival of trichomonas vaginalis in human semen. | although exposure of trichomonas vaginalis to human semen is of short duration, any effect that this fluid may have on the urogenital protozoon could affect its transmission, especially if only few trichomonads are present. small numbers of parasites (about 2500/ml semen) incubated in semen from different donors at 37 degrees c, were found to survive or grow for up to 12 hours in all samples and for up to 24 hours in most. survival and growth of t vaginalis in semen most resembled that found in ... | 1989 | 2787774 |
| evaluation of six media for the growth of trichomonas vaginalis from vaginal secretions. | many media have been formulated for the growth of trichomonas vaginalis, but the relative sensitivities of these media have not been determined. we evaluated the ability of six media, including all five media commercially available in the united states, to grow trichomonas vaginalis from vaginal secretions. in a first experiment, we evaluated the ability of five media to grow t. vaginalis from vaginal secretions of 375 women and determined the optimal days on which to read culture tubes, by inoc ... | 1989 | 2787804 |
| trichomonas vaginalis and tritrichomonas foetus secrete neuraminidase into the culture medium. | supernatants taken from axenic cultures of trichomonas vaginalis and tritrichomonas foetus contain a neuraminidase activity, the detection of which is augmented when the trichomonad culture media are supplemented with 30% supernatant of confluent epithelial cultures. the enzyme was active against human erythrocytes, which became highly reactive to peanut agglutinin lectin. the specificity of the enzyme was checked by using a substrate specific to neuraminidase: 2'-(4-methylumbelliferyl)-alpha-d- ... | 1989 | 2788244 |
| characterization of a secreted cytoactive factor from trichomonas vaginalis. | trichomonas vaginalis, grown in dulbecco's modified eagle's medium with or without serum, produced a factor (tvf) which altered the morphology of certain mammalian cells in vitro. tvf had a mr of approximately 250 kda by gel filtration, approximately 50 kda by sds-page, and was heat (56 degrees c, 30 min) and ph (greater than 6 or less than 8) labile. co-incubation of tvf with adherent target cells caused a marked rounding and clumping of bhk-21 or cho-k1 cells, but had no effect on rk-13 or weh ... | 1989 | 2788372 |
| isolation of a cell-detaching factor of trichomonas vaginalis. | the pathogenetic role of soluble products of trichomonas vaginalis growth in culture is controversial. to evaluate this role, t. vaginalis was grown in broth and cell culture and the cell-free filtrate was applied to fresh cell culture monolayers. when adjusted to ph 6.5, filtrates obtained from 22-h culture growth totally disrupted mccoy, hep-2, human foreskin fibroblast, and chinese hamster ovary cell monolayers within 6 h. these detached cells remained greater than 90% viable. this cell-detac ... | 1989 | 2788661 |
| trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. | the role of cysteine proteinases in adherence of trichomonas vaginalis nyh 286 to hela and human vaginal epithelial cells was evaluated. only pretreatment of trichomonads, but not epithelial cells, with n-alpha-p-tosyl-l-lysine chloromethyl ketone (tlck), an inhibitor of trichomonad cysteine proteinases, greatly diminished the ability of t. vaginalis to recognize and bind to epithelial cells. leupeptin and l-1-tosylamide-2-phenylethyl chloromethyl ketone, other cysteine proteinase inhibitors, al ... | 1989 | 2789190 |
| identification of virus-like particles in eimeria stiedae. | when nucleic acid samples purified from sporozoites of eimeria stiedae were analyzed by agarose gel electrophoresis, an ethidium-stainable band with an apparent electrophoretic mobility of 6.5 kb was consistently observed. the band was readily degradable upon rnase treatment, and its susceptibility towards ribonuclease a on a decreasing ionic strength was suggestive of double-stranded rna (dsrna). electron microscopy revealed spherical, probably icosahedral, virus-like particles (vlp) with a dia ... | 1989 | 2797059 |
| [the microbial flora of the vagina in trichomoniasis]. | the vaginal flora was investigated in women with trichomoniasis by means of a culture method. trichomonas, yeast-like fungi and various types of bacteria were isolated. the applied microbiological technique allowed isolation not only of aerobic and cicrophilic bacteria but only of anaerobic bacteria. anaerobic bacteria were nonspore-forming and were isolated very frequently in the vagina. | 1989 | 2802080 |
| failure of mebendazole to cure trichomonal vaginitis resistant to metronidazole: case reports. | after a recent report showing the in vitro susceptibility to mebendazole of a strain of trichomonas vaginalis that was resistant to metronidazole, we present two cases of metronidazole resistant infection, both of which failed to respond to oral mebendazole. | 1989 | 2807288 |
| sexually transmitted pathogens in pregnant women in a rural south african community. | one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ... | 1989 | 2807289 |
| chlamydia trachomatis cervical infection and oral contraceptive use among adolescent girls. | this study examines the relationship between oral contraceptive use and chlamydia trachomatis cervical infection in women less than 19 years of age. the clinical and epidemiologic data of 73 (19.4% prevalence rate) girls with chlamydial infection were analyzed, with special attention given to contraceptive use. the findings were compared with data from 303 girls who were chlamydia negative to assess the relationship between oral contraceptive use and c. trachomatis infection. use of an oral cont ... | 1989 | 2808080 |
| characterization and purification of extracellular proteases of trichomonas vaginalis. | extracellular protease activity was detected in serum-free culture filtrates of trichomonas vaginalis. the activity was demonstrated by hydrolysis of hide powder azure and possessed the characteristics of cysteine type proteases: inhibition by n-ethyl maleimide, cu2+, antipain, n-tosyl-l-phenylalanine chloromethyl ketone, n-tosyl-l-lysine chloromethyl ketone, leupeptin, chymostatin, and iodoacetamide, and enhancement by cysteine, edta, and dithiothreitol. the activity was optimal at acid ph and ... | 1989 | 2819601 |
| commentary: family planning and sex education in std. | the incidence of sexually transmitted diseases (std) has risen in the past years to epidemic proportions even though medical understanding of stds has considerably improved. the most common forms of contraception (e.g. condoms, diaphragms, vaginal spermicides, intrauterine devices, and oral contraception pills) vary in how effective each is in preventing stds. condoms offer almost complete protection against many stds, including, gonorrhoea, syphilis, herpes virus, trichomonas, candidiases, ch ... | 1989 | 12283075 |
| the effect of trichomonas vaginalis and the role of ph on cell culture monolayer viability. | in vitro, trichomonas vaginalis produces contact-dependent cytotoxicity in which the monolayers are disrupted and monolayer cells die. in contrast, when cell-free filtrates of t. vaginalis culture supernatants are applied to a monolayer, detachment occurs but cell viability is maintained. growth of t. vaginalis in culture results in a dramatic fall in ph. both in systems where t. vaginalis grew in physical contact with mccoy cells and where they were physically separate, monolayers still disrupt ... | 1990 | 1693888 |
| molecular analysis of the hydrogenosomal ferredoxin of the anaerobic protist trichomonas vaginalis. | we have determined the primary structure of the [2fe-2s]ferredoxin of the anaerobic protist trichomonas vaginalis. this protein, situated in the hydrogenosome, is composed of 93 amino acids. a comparison of t. vaginalis ferredoxin with greater than 80 other ferredoxins shows the closest similarity to [2fe-2s]putidaredoxin of the aerobic bacterium pseudomonas putida and a lesser one to mitochondrial [2fe-2s]ferredoxins of vertebrates. this similarity is reflected in the overall primary structure ... | 1990 | 1696716 |
| effect of fixation on activity and cytochemistry of hydrogenosomal enzymes in trichomonas vaginalis. | the effect of fixation on the activity of malate dehydrogenase (decarboxylating) and pyruvate synthase was investigated in trichomonas vaginalis. subsequently a cytochemical staining method was developed for the demonstration of malate dehydrogenase activity in hydrogenosomes. after fixation of cells in low concentrations of glutaraldehyde and incubation in the presence of malate and the tetrazolium compound 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl)tetrazolium chloride, an electron- ... | 1990 | 1706759 |
| glutathione transferase activity in some flagellates and amoebae, and purification of the soluble glutathione transferases from acanthamoeba. | glutathione transferase (gst) enzymes are toxicologically important from many points of view. nine protozoans were investigated here for their gst content. six aerobic amoebae had very different specific gst activities, but an anaerobic amoeba and two anaerobic flagellates did not have any gst activity, suggesting that the peroxidase activity of gst is an evolutionarily important property for aerobic organisms. the soluble gst isoenzymes of acanthamoeba culbertsoni and a. polyphaga were purified ... | 1990 | 2078188 |
| [antiprotozoal effects of benzodiazepine derivatives]. | it is known that some derivatives of the benzodiazepine group act not only as antipsychotic drugs but also have inhibitory effects on the growth of protozoa. the influence of imipramine and its derivatives clomipramine and desipramine on the multiplication of crithidia luciliae, leishmania mexicana amazonensis, trypanosoma cruzi and trichomonas vaginalis was investigated using in vitro cultivation of the parasites and compared with the effects of chlorpromazine and metronidazole. all trypanosoma ... | 1990 | 2085212 |
| sexually transmitted diseases and rape: the experience of a sexual assault centre. | epidemiological data and rates of sexually transmitted diseases (stds) are presented from a group of 90 women screened following rape. forty-eight (53%) women knew their assailant. in 6 cases multiple assailants were involved. in 21 (23%) rapes weapons were used. of 47 women examined within 48 hours of rape, 17 (36%) had evidence of genital trauma. anal injury was common (8 of 11, 73%) in those reporting anal intercourse. anal assault (17 of 90, 19%) and oral assault (16 of 90, 18%) commonly acc ... | 1990 | 2094402 |
| a cytotoxic effect of h2o2 on trichomonas vaginalis may be correlated to cytoskeleton modification. | 1990 | 2097657 | |
| treatment failure in trichomoniasis and persistence of the parasite after lactobacillus immunotherapy; two case reports. | this report describes the clinical and laboratory observations on two patients with a trichomonas vaginalis infection resistant to metronidazole. the metronidazole resistance was confirmed in in vitro cultures under aerobic conditions, after in vitro cultivation of the strains. trichomonas infection persisted during high-dose intravenous metronidazole administration and lactobacillus immunotherapy was unsuccessful in both patients. | 1990 | 2105899 |
| pattern of genito-urinary parasitic infections at the teaching hospital, sokoto, nigeria. | urinary schistosomiasis was more frequently observed among subjects aged 10-19 years old; and the degree of exposure to the infection was found to be high among the males. comparative analysis of t. vaginalis infection among the females revealed a ten-fold increase in the detection rate for the flagellate in the urine samples collected from the special treatment clinic than those from out-patient department. the age-specific morbidity pattern for trichomonal vaginitis was remarkably high among f ... | 1990 | 2112996 |
| a microbiological study of failed penicillin therapy for gonococcal urethritis in durban. | seventy-five men presenting with persistent urethral discharge after penicillin therapy were investigated for sexually transmitted pathogens during july - september 1987. the major aetiological agent isolated was neisseria gonorrhoeae (58 patients (77.3%]. penicillinase-producing n. gonorrhoeae (ppng) accounted for 58.6% of 58 isolates. chlamydia trachomatis was detected in 10.7% of patients and trichomonas vaginalis in 14.7% of patients. when there is a high prevalence of ppng, the use of penic ... | 1990 | 2116671 |
| trichomonas vaginalis requires traces of oxygen and high concentrations of carbon dioxide for optimal growth. | the effects of o2 and co2 on the growth in culture of trichomonas vaginalis strain c1-nih were investigated. growth under pre-purified n2 in the absence of co2 supplementation gave a doubling time of 4.4 h; when traces of o2 (less than 0.25 microm) were present, the doubling time was 3.5 h. organisms grew most rapidly (doubling time 2.3 h) with traces of o2 (less than 0.25 microm) and with the co2 level controlled at 5 mm. the balance of fermentation products from maltose was greatly influenced ... | 1990 | 2117256 |
| a further look at infection at the time of therapeutic abortion. | microbiological investigations and clinical follow-up were performed on 516 patients undergoing first-trimester termination of pregnancy, over a 2-year period. chlamydia trachomatis was isolated by pre-operative microbiological screening in 6.7% of patients, gonococcus in 0.4% and trichomonas vaginalis in 2%. candida spp and gardnerella vaginalis were isolated from 16% and 28% of the women, respectively. patients who had positive cervical chlamydial isolation were significantly younger (less tha ... | 1990 | 2121562 |
| neisseria gonorrhoeae, trichomonas vaginalis, and yeast reported from attenders at an antenatal clinic in a rural area in botswana. | 1990 | 2125025 | |
| in vitro antimicrobial activity of econazole and miconazole sulfosalicylate. | the in vitro activities of new sulfosalicylic salts of econazole (e.ssa) and miconazole (m.ssa) have been investigated in comparison with the respective nitrate (nit) salts and sulfosalicylic acid (ssa) alone. the results reveal good antimicrobial activity of m.ssa and e.ssa against different strains of candida, dermatophytes, moulds, gram-positive bacteria and trichomonas vaginalis. the mic values demonstrate that m.ssa is more active than m.nit on candida and t. vaginalis. e.ssa was also more ... | 1990 | 2127740 |
| effects of berberine, a plant alkaloid, on the growth of anaerobic protozoa in axenic culture. | extracts obtained by organic solvents from the root of coptis teeta in myanmar (burma), were tested for growth inhibitory activity against giardia lamblia, trichomonas vaginalis and entamoeba histolytica in axenic culture. all extracts had anti-protozoal activity with the methanol extract, in particular, being effective against all 3 parasites. by chromatographic analysis, the extracts were shown to contain berberine compounds and other alkaloids. comparing the inhibitory effects of the methanol ... | 1990 | 2131648 |
| [structure and evolution of trichomonadida]. | in the article the survey of data on morphology and ultrastructure of flagellates of the order trichomonadida is given with special attention paid to monocercomonadidae and trichomonadidae. the evolution within these flagellates is considered as a series of changes leading to formation of undulating membrane and reinforcement of the cytoskeleton. in both families other two evolutionary tendencies may be also observed: reduction of the flagellar apparatus (in monocercomonadidae) and enlargement o ... | 1990 | 2131689 |
| [effect of pseudomonas aeruginosa on trichomonas vaginalis in vitro--electron-microscopic studies]. | interaction between t. vaginalis and p. aeruginosa was studied in vitro. protozoan cells were incubated in 0.9% nacl at 37 degrees c for 30 and 150 min. with bacteria, alive or killed by heating for 10 min. at 100 degrees c. transmission electron microscope observations showed weak ++phagocytic activity (less than 10%) of t. vaginalis against living p. aeruginosa. phagocytosis of these bacteria caused death of t. vaginalis. disintegration and autolysis of t. vaginalis resulted probably from acti ... | 1990 | 2131691 |
| [incidence of trichomonas vaginalis infection among girls from the lódź population in the last 25-year period]. | 19,024 girls (aged: from 0 to 18) attending the pediatric gynaecology outpatient department and the center for treatment of parasitic and mycotic diseases were examined. the diagnosis of infection with t. vaginalis was based on preparations using various methods and cultures on specific media, from vaginal content and urine. it was found that the frequency of t. vaginalis invasions in girls had lessened 2-6 times in different groups from 1960 till 1985 and the higher percent of t. vaginalis in g ... | 1990 | 2131692 |
| [several years of observation of the incidence of trichomonas vaginalis infections among female workers in a single industrial plant]. | during 5 years cycle a prophylactic gynaecological, cytological and parasitological examinations of 1918 women were performed. 12.8% of women were infected with t. vaginalis. decreasing percentage of infections was observed: from 9.92% in 1984 to 2.2% in 1988. in the i. of papanicolaou cervical smears t. vaginalis has been found in 1.60% of cases, in the ii.--in 5.2%, in the iii.--in 14.29%, and in the iv.--in 22.22% of cases. in women with normal cervix uteri t. vaginalis have been found in 5.0 ... | 1990 | 2131693 |
| [trichomonas vaginitis at different life stages of women]. | using of fresh slides and culture t. vaginalis was found in 1094 persons (3.20%). it has been seen the morphological differentiation of t. vaginalis connected with the age, physiological state of macroorganisms and the clinic picture of trichomoniasis. in vivo, the spheroidal as well as ameboid forms of t. vaginalis were observed. the latter ones, characteristic in acute trichomoniasis, were often seen in pregnant women. on the other hand, nonmobile round-shaped forms of t. vaginalis occurred in ... | 1990 | 2131695 |
| [analysis of the relations between selected symptoms of inflammation of the genital and urinary organs and invasion of trichomonas vaginalis]. | data concerning 604 girls up 18 year old were analysed. on the basis clinical symptoms and results of culture of various biological materials on selected media--the monofocal trichomonas infection concerning the most often vulva and vagina, rarely urethra or other regions of urinary organ as well as the multifocal trichomonas infection, particularly complicated by mycosis were proved. it was found the convergence statistically significant (p less than or equal to 0.5) of the invasion of t. vagin ... | 1990 | 2131696 |
| [rare cases of prostatitis caused by invasion of trichomonas vaginalis with candida albicans]. | three cases of prostatitis caused by the invasion of t. vaginalis and c. albicans which has been found in different biological materials were described. after per rectum examination perineum biopsy of prostata was performed in all patients; in histopathological preparations pointing at the inflammation reaction of that gland the fungi were detected. also, the same microorganisms were proved in sexual partners of those patients but multifocal invasion of c. albicans--including genital and urinary ... | 1990 | 2131697 |
| [studies of compounds with potential antiparasitic action. viii. new oxime ethers in the 1-thiochromone system]. | the 50% antitrichomonal concentration of 7 examined ethers (t. vaginalis strain n. 1/86, roiron medium, safranine staining, 30 min.) was estimated by means of dose-response curve. the minimal mycostatic concentration (candida albicans l-45, geotrichum candidum; 3% sabouraud agar, 37 degrees c, 24 hr) was calculated with the aid of regression equation. all examined ethers show in vitro a marked antitrichomonal effect comparable with activity of ornidazole or phenol. mycostatic activity of new com ... | 1990 | 2131701 |
| [antitrichomonal properties of derivatives of ethers containing amine and halogenarylalkyl groups]. | the trichomonacidal activity in vitro of 6 new derivatives of theirs containing amine and halogenaryloalkyl groups in comparison with metronidazole, ornidazole and tinidazole has been examined. for all the examined compounds curves of action were obtained after 30 min, and lethal concentrations for 50% population (cl50) of three strains of t. vaginalis used for experiments were calculated. the trichomonacidal action of the following compounds was specially strong: 2-/1-methylpiperazine++-4-yl/et ... | 1990 | 2131703 |
| [in vitro sensitivity of entamoeba histolytica to metronidazole]. | the chemotherapy of amebiasis includes different substances with intra and extraintestinal action. nowadays, metronidazole is the election drug by its high efficacy and low toxicity. some therapeutic failures with metronidazole in patients with invader amebiasis and some reports of resistance to it, of certain strains of trichomonas vaginalis and species of bacteroides have caused an increased interest to do new in vitro evaluation of this compound and to search of new antiamebic drugs. the obje ... | 1990 | 2136490 |
| fibronectin- and laminin-mediated endocytic activity in the parasitic protozoa trichomonas vaginalis and tritrichomonas foetus. | the pathogenic protozoan tritrichomonas foetus is able to ingest polystyrene particles with a diameter up to 1.0 micron. trichomonas vaginalis, however, ingest particles as large as 4.4 microns in diameter. the particles are found within cytoplasmic membrane-bounded vacuoles. morphometrical analysis showed that t. vaginalis presents a higher endocytic activity than t. foetus. coating of the polystyrene particles with cationized ferritin increases their binding to the parasite surface but does no ... | 1990 | 2138055 |
| structural and histoenzymatic aspects of metronidazol on rat gonads. | metronidazol, an efficient drug for trichomonas vaginalis, lamblia intestinalis and some anaerobic germs, administered in a daily dose of 500 mg/kg body weight for 60 and 40 days in males and females, respectively, produces severe structural alterations (atrophy and cytolysis, more apparent in the testicle) and elicits, a functional impairment as well (modifications in the activity of some enzymes: alkaline and acide-phosphatase, mg(2+)-atpase, cyox, sdh, ldh). these morphological and histoenzym ... | 1990 | 2151289 |
| freeze-fracture study of trichomonas vaginalis. | the freeze-fracture technique was used to analyse the organization of the plasma membrane, as well as membranes of cytoplasmic organelles, of the pathogenic protozoan trichomonas vaginalis. rosettes formed by 4 to 14 intramembranous particles were seen on the fracture faces of the membrane lining the anterior flagella as well as in fracture faces of the plasma membrane enclosing the anterior region of the protozoan and in cytoplasmic organelles. special organization of the membrane particles wer ... | 1990 | 2152194 |
| sexually transmitted diseases in victims of rape. | the risk of acquiring a sexually transmitted disease as a result of rape is not known, in part because it is difficult to ascertain whether infections were present before the assault or acquired during it. to investigate this question, we examined female victims of rape within 72 hours of the assault and again at least one week after the assault. of the 204 girls and women initially examined within 72 hours of the rape, 88 (43 percent) were found to have at least one sexually transmitted disease ... | 1990 | 2155389 |
| cytomegalovirus infection among women attending a sexually transmitted disease clinic: association with clinical symptoms and other sexually transmitted diseases. | the prevalence rates of cytomegalovirus, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, and herpes simplex virus infection were determined for 247 women attending a sexually transmitted disease clinic in halifax, nova scotia between july 1983 and december 1985. isolation rates were 8.5%, 32.8%, 27.1%, 7.3%, and 6.5% for the five infectious agents, respectively. with multiple logistic regression analysis, the presence of cervical cytomegalovirus infection was independently a ... | 1990 | 2156421 |
| inhibition of trichomonas vaginalis motility by monoclonal antibodies is associated with reduced adherence to hela cell monolayers. | adherence of trichomonads to host epithelial cells appears to be a critical step in the pathogenesis of trichomoniasis. we evaluated the effect of a panel of 10 monoclonal antibodies on attachment of [35s]methionine-radiolabeled trichomonas vaginalis strains to hela cell monolayers. of 10 monoclonal antibodies, 3 totally eliminated motility of phs2j strain trichomonads and reduced their adherence to 48 to 60% of control values (p less than 0.001). however, none of the monoclonal antibodies affec ... | 1990 | 2187803 |