Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation of pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion. | each year, approximately 170 million metric tons of chemical fertilizer are consumed by global agriculture. furthermore, some chemical fertilizers contain toxic by-products and their long-term use may contaminate groundwater, lakes, and rivers. the use of plant growth-promoting bacteria may be a cost-effective strategy for partially replacing conventional chemical fertilizers, and may become an integrated plant nutrient solution for sustainable crop production. the main direct bacteria-activated ... | 2015 | 26634494 |
| antifungal properties of rhamnolipid produced by pseudomonas aeruginosa ds9 against colletotrichum falcatum. | the rhamnolipid biosurfactant (rl-ds9) extracted from the bacterial strain pseudomonas aeruginosa ds9 was evaluated for its antifungal activity against colletotrichum falcatum that causes red rot in sugarcane. the surface tension (st) reduction, biosurfactant production, and antifungal activity of biosurfactant against c. falcatum were investigated by using the medium with different carbon sources and it was found to be maximum in glucose. moreover, highest reduction of st and production of bios ... | 2015 | 26173581 |
| xylanases of bacillus spp. isolated from ruminant dung as potential accessory enzymes for agro-waste saccharification. | polysaccharide hydrolase producing bacteria were isolated for biomass saccharification step in two-step bioethanol production. xylanolytic bacteria were found to be common in ruminant dung. seven bacillus dung isolates exhibited high xylanolytic activity; three of which were identified as bacillus safensis and four as bacillus altitudinis, based on 16s rdna and gyrb gene sequencing. interestingly, comparison of activity profiles for b. safensis m35 and b. altitudinis r31 and j208 crude xylanases ... | 2015 | 25645626 |
| draft genome sequence of bacillus amyloliquefaciens jjc33m, isolated from sugarcane soils in the papaloapan region, mexico. | bacillus amyloliquefaciens strain jjc33m is a bacterium that produces α-amylase (ec 3.2.1.1) and was isolated from sugarcane soil. its estimated genome size is 3.96 mb, and it harbors 4,048 coding genes (cdss). | 2015 | 25676753 |
| poly-γ-glutamic acid produced from bacillus licheniformis cgmcc 2876 as a potential substitute for polyacrylamide in the sugarcane industry. | as an environmentally friendly and industrially useful biopolymer, poly-γ-glutamic acid (γ-pga) from bacillus licheniformis cgmcc 2876 was characterized by the high-resolution mass spectrometry and (1)h nmr. a flocculating activity of 11,474.47 u ml(-1) obtained with γ-pga, and the effects of carbon sources, ions, and chemical properties (d-/l-composition and molecular weight) on the production and flocculating activity of γ-pga were discussed. being a bioflocculant in the sugar refinery process ... | 2015 | 26033934 |
| increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from bacillus licheniformis. | the use of endo-arabinanase from bacillus licheniformis (abnase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein. | 2015 | 25801671 |
| simultaneous saccharification and fermentation of delignified lignocellulosic biomass at high solid loadings by a newly isolated thermotolerant kluyveromyces sp. for ethanol production. | simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5-15% solid loading and 6-12 fpu g(-1) substrate enzyme loading for different time intervals 0-72 h at 42°c. maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from aspergillus terreus was 23.23, 18.29 and 1 ... | 2015 | 25553563 |
| gh11 xylanase from emericella nidulans with low sensitivity to inhibition by ethanol and lignocellulose-derived phenolic compounds. | an endo-β-1,4-xylanase (x22) was purified from crude extract of emericella nidulans when cultivated on submerged fermentation using sugarcane bagasse as the carbon source. the purified protein was identified by mass spectrometry and was most active at ph and temperature intervals of 5.0-6.5 and 50-60°c, respectively. the enzyme showed half-lives of 40, 10 and 7 min at 28, 50 and 55°c, respectively, and ph 5.0. apparent km and vmax values on soluble oat spelt xylan were 3.39 mg/ml and 230.8 iu/mg ... | 2015 | 26040589 |
| lepidoptera (crambidae, noctuidae, and pyralidae) injury to corn containing single and pyramided bt traits, and blended or block refuge, in the southern united states. | fall armyworm, spodoptera frugiperda (j. e. smith) (lepidoptera: noctuidae); corn earworm, helicoverpa zea boddie (lepidoptera: noctuidae); southwestern corn borer, diatraea grandiosella dyar (lepidoptera: crambidae); sugarcane borer, diatraea saccharalis f. (lepidoptera: crambidae); and lesser cornstalk borer, elasmopalpus lignosellus zeller (lepidoptera: pyralidae), are lepidopteran pests of corn, zea mays l., in the southern united states. blended refuge for transgenic plants expressing the i ... | 2015 | 26470116 |
| identification, inheritance, and fitness costs of cry2ab2 resistance in a field-derived population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is one of the major target pests of transgenic maize, zea mays, expressing bacillus thuringiensis (bt) proteins in south america and mid-southern region of the u.s. the mon89034 maize expresses cry2ab2 and cry1a.105 bt proteins and it was developed to provide better control of key lepidopteran pests of maize including d. saccharalis. the objectives of this study were to select and characterize the resistance of d. saccharalis to cry2ab2 using a non ... | 2015 | 26205174 |
| binding analysis of bacillus thuringiensis cry1 proteins in the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | sugarcane borer (diatraea saccharalis, f.) is an important corn pest in south america and united states. the aim of the present study was to analyze the susceptibility and binding interactions of three cry1a proteins and cry1fa in a brazilian d. saccharalis population. the results showed that cry1ab was the most active, followed by cry1ac, cry1fa and cry1aa. all cry1-biotinylated proteins tested bound specifically to the d. saccharalis brush border membrane vesicles (bbmv). heterologous competit ... | 2015 | 25736726 |
| sugarcane bagasse as support for immobilization of bacillus pumilus hz-2 and its use in bioremediation of mesotrione-contaminated soils. | the degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. in the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. a novel isolate bacillus pumilus hz-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrat ... | 2015 | 26337896 |
| isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from bacillus vallismortis rg-07. | the rising concerns about the scarcity of fossil fuels, the emission of green house gasses and air pollution by incomplete combustion of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. the aim of this study was to isolate a potential thermo-solvent tolerant cellulase producing bacterium from natural resources, and then applied for purification and characterization. th ... | 2015 | 25886936 |
| [succinic acid production from sucrose and sugarcane molasses by metabolically engineered escherichia coli]. | sugarcane molasses containing large amounts of sucrose is an economical substrate for succinic acid production. however, escherichia coli afp111 cannot metabolize sucrose although it is a promising candidate for succinic acid production. to achieve sucrose utilizing ability, we cloned and expressed cscbka genes encoding sucrose permease, fructokinase and invertase of non-pts sucrose-utilization system from e. coli w in e. coli afp111 to generate a recombinant strain afp111/pmd19t-cscbka. after 7 ... | 2015 | 26380410 |
| improved waste water treatment by bio-synthesized graphene sand composite. | the photocatalytic and antibacterial properties of graphene biosynthesized from sugar and anchored on sand particles has been focused here. the morphology and composition of the synthesized graphene sand composite (gsc) was investigated by means of x-ray powder diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edax), fourier transform infra-red spectroscopy (ftir) and uv-visible spectroscopy. sem images show wrinkly edges. this is characteristic of grap ... | 2015 | 26265599 |
| improved ethanol production from biomass by a rumen metagenomic dna fragment expressed in escherichia coli ms04 during fermentation. | with the aim of improving current ethanologenic escherichia coli strains, we screened a metagenomic library from bovine ruminal fluid for cellulolytic enzymes. we isolated one fosmid, termed csd4, which was able to confer to e. coli the ability to grow on complex cellulosic material as the sole carbon source such as avicel, carboxymethyl cellulose, filter paper, pretreated sugarcane bagasse, and xylan. glucanolytic activity obtained from e. coli transformed with csd4 was maximal at 24 h of incub ... | 2015 | 26175105 |
| mutation in galp improved fermentation of mixed sugars to succinate using engineered escherichia coli as1600a and am1 mineral salts medium. | escherichia coli kj122 was engineered to produce succinate from glucose using the wild type galp for glucose uptake instead of the native phosphotransferase system (ptsi mutation). this strain now ferments 10% xylose poorly. mutants were selected by serial transfers in am1 mineral salts medium with 10% xylose. clones from this population all exhibited a similar improvement, co-fermentation of an equal mixture of xylose and glucose. one of these, as1600a, produced 84.26 ± 1.37 g/l succinate, equi ... | 2015 | 26159300 |
| overexpression and characterization of a glucose-tolerant β-glucosidase from t. aotearoense with high specific activity for cellobiose. | thermoanaerobacterium aotearoense p8g3#4 produced β-glucosidase (bgl) intracellularly when grown in liquid culture on cellobiose. the gene bgl, encoding β-glucosidase, was cloned and sequenced. analysis revealed that the bgl contained an open reading frame of 1314 bp encoding a protein of 446 amino acid residues, and the product belonged to the glycoside hydrolase family 1 with the canonical glycoside hydrolase family 1 (gh1) (β/α)8 tim barrel fold. expression of pet-bgl together with a chaperon ... | 2015 | 25957152 |
| the antibiotic activity and mechanisms of sugarcane (saccharum officinarum l.) bagasse extract against food-borne pathogens. | sugarcane bagasse contains natural compositions that can significantly inhibit food-borne pathogens growth. in the present study, the phenolic content in sugarcane bagasse was detected as higher than 4 mg/g dry bagasse, with 470 mg quercetin/g polyphenol. the sugarcane bagasse extract showed bacteriostatic activity against the growth of staphylococcus aureus, listeria monocytogenes, escherichia coli and salomonella typhimurium. additionally, the sugarcane bagasse extract can increase the electri ... | 2015 | 25952848 |
| combining treatments to improve the fermentation of sugarcane bagasse hydrolysates by ethanologenic escherichia coli ly180. | inhibitory side products from dilute acid pretreatment is a major challenge for conversion of lignocellulose into ethanol. six strategies to detoxify sugarcane hydrolysates were investigated alone, and in combinations (vacuum evaporation of volatiles, high ph treatment with ammonia, laccase, bisulfite, microaeration, and inoculum size). high ph was the most beneficial single treatment, increasing the minimum inhibitory concentration (measured by ethanol production) from 15% (control) to 70% hydr ... | 2015 | 25864026 |
| complete genome sequence of nine isolates of canna yellow streak virus reveals its relationship to the sugarcane mosaic virus (scmv) subgroup of potyviruses. | complete genome sequences were obtained from nine isolates of canna yellow streak virus (caysv). caysv belongs to the sugarcane mosaic virus (scmv) subgroup of potyviruses with johnsongrass mosaic virus (jgmv) as its closest relative. multiple sequence alignments showed a pattern of amino acid substitutions in the cp sequences, which enabled us to relate these isolates to south east asian or european isolates. biological characterization of caysv identified nicotiana benthamiana, chenopodium qui ... | 2015 | 25567205 |
| xylitol production by yeasts isolated from rotting wood in the galápagos islands, ecuador, and description of cyberlindnera galapagoensis f.a., sp. nov. | this study evaluated d-xylose-assimilating yeasts that are associated with rotting wood from the galápagos archipelago, ecuador, for xylitol production from hemicellulose hydrolysates. a total of 140 yeast strains were isolated. yeasts related to the clades yamadazyma, kazachstania, kurtzmaniella, lodderomyces, metschnikowia and saturnispora were predominant. in culture assays using sugarcane bagasse hemicellulose hydrolysate, candida tropicalis clqca-24sc-125 showed the highest xylitol producti ... | 2015 | 26219566 |
| truncated ubiquitin 5' regulatory region from erianthus arundinaceus drives enhanced transgene expression in heterologous systems. | characterization of novel plant gene promoters underpins the development of transgenic crop plants. here, we report a novel 5' regulatory sequence (eriubi d7) of the ubiquitin gene from erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. a 3.2-kb regulatory sequence of ubiquitin gene was isolated through random amplification of genomic ends technique and characterized in rice, tobacco, and sugarcane. in silico analysis revealed that the regulatory ... | 2015 | 25985900 |
| loop-mediated isothermal amplification (lamp) based detection of colletotrichum falcatum causing red rot in sugarcane. | red rot, caused by colletotrichum falcatum, is a destructive disease prevalent in most sugarcane-producing countries. disease-free sugarcane planting materials (setts) are essential as the pathogen spreads primarily through infected setts. the present study was undertaken to develop a loop-mediated isothermal amplification (lamp) assay for the detection of c. falcatum. c. falcatum genomic dna was isolated from pure mycelium culture and infected tissues. four sets of primers corresponding to a un ... | 2015 | 25861736 |
| fvbck1, a component of cell wall integrity map kinase pathway, is required for virulence and oxidative stress response in sugarcane pokkah boeng pathogen. | fusarium verticillioides (formerly f. moniliforme) is suggested as one of the causal agents of pokkah boeng, a serious disease of sugarcane worldwide. currently, detailed molecular and physiological mechanism of pathogenesis is unknown. in this study, we focused on cell wall integrity mapk pathway as one of the potential signaling mechanisms associated with pokkah boeng pathogenesis. we identified fvbck1 gene that encodes a map kinase kinase kinase homolog and determined that it is not only requ ... | 2015 | 26500635 |
| deciphering the transcriptomic response of fusarium verticillioides in relation to nitrogen availability and the development of sugarcane pokkah boeng disease. | pokkah boeng, caused by fusarium verticillioides, is a serious disease in sugarcane industry. the disease severity is related to the sugarcane genotype as well as environmental considerations, such as nitrogen application. the impact of the nitrogen source (ammonium sulfate, urea, or sodium nitrate) on sugarcane pokkah boeng disease and its pathogen was investigated in planta and fungal growth and sporulation production was measured in vitro. the results showed that ammonium and nitrate were ben ... | 2016 | 27434999 |
| the decrease in the population of gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments. | it has been established that a decrease in the population of gluconacetobacter diazotrophicus associated with sugarcane occurs after nitrogen fertilization. this fact could be due to a direct influence of nh(4)no(3) on bacterial cells or to changes in plant physiology after fertilizer addition, affecting bacterial establishment. in this work, we observed that survival of g. diazotrophicus was directly influenced when 44.8mm of nh(4)no(3) (640mgn/plant) was used for in vitro experiments. furtherm ... | 2016 | 26652262 |
| striga biocontrol on a toothpick: a readily deployable and inexpensive method for smallholder farmers. | striga hermonthica (witchweed) is a parasitic weed that attacks and significantly reduces the yields of maize, sorghum, millet, and sugarcane throughout sub-saharan africa. low cost management methods such as hand weeding, short crop rotations, trap cropping, or conventional biocontrol have not been effective. likewise, striga-tolerant or herbicide-resistant maize cultivars are higher yielding, but are often beyond the economic means of sustenance farmers. the fungal pathogen, fusarium oxysporum ... | 2016 | 27551284 |
| development and application of a rapid and visual loop-mediated isothermal amplification for the detection of sporisorium scitamineum in sugarcane. | smut is a fungal disease with widespread prevalence in sugarcane planting areas. early detection and proper identification of sporisorium scitamineum are essential in smut management practices. in the present study, four specific primers targeting the core effector pep1 gene of s. scitamineum were designed. optimal concentrations of mg(2+), primer and bst dna polymerase, the three important components of the loop-mediated isothermal amplification (lamp) reaction system, were screened using a sin ... | 2016 | 27035751 |
| fusarium sacchari, a cause of mycotic keratitis among sugarcane farmers - a series of four cases from north india. | the two most common filamentous fungi causing mycotic keratitis are aspergillus and fusarium spp. around 70 fusarium spp. are involved in causing human infections. in this study, four cases of keratitis in sugarcane farmers in india are being reported, caused by the sugar cane pathogen fusarium sacchari, a species of the fusarium fujikuroi species complex. fusarial keratitis was established by potassium hydroxide/calcofluor white wet mounts and fungal culture of corneal scrapings on conventional ... | 2016 | 27292696 |
| a betabaculovirus encoding a gp64 homolog. | a betabaculovirus (disagv) was isolated from diatraea saccharalis (lepidoptera: crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in brazil. | 2016 | 26847652 |
| parameters of fermentation and rumen microbiota of nellore steers fed with different proportions of concentrate in fresh sugarcane containing diets. | the objective of this study was to evaluate the effect of a fresh sugarcane-based diet and different roughage-to-concentrate ratios (70:30, 60:40, 40:60 and 20:80) on the rumen microbiota associated with rumen fermentation parameters and the intake and apparent digestibility of nutrients in nellore steers. eight rumen-cannulated nellore steers (331 ± 8 kg bw) were distributed in a double 4 × 4 latin square design balanced for the control of the residual effect. the ruminal ph decreased (p < 0.01 ... | 2016 | 27415825 |
| fungal communities in pressmud composting harbour beneficial and detrimental fungi for human welfare. | pressmud is a substrate derived from sugarcane juice filtrate, and around 26-40 kg of this residue are produced per ton of sugarcane. it is mainly used as fertilizer in crops, and its application in the field is often made without any prior treatment, but, in this research, it was studied for the risk this practice poses for human health. this research was stimulated by previous results indicating the presence of opportunistic pathogens in residues used in various composting systems and the ext ... | 2016 | 27170376 |
| ima genome-f 6: draft genome sequences of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica and penicillium freii daomc 242723. | the genomes of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica, and penicillium freii daomc 242723 are presented in this genome announcement. these six genomes are from plant pathogens and otherwise economically important fungal species. the genome sizes range from 21 mb in the case of ceratocystiopsis minuta to 58 mb for the basidiomycete armillaria fuscipes. these genomes include the first reports of genomes for the genus endoconidio ... | 2016 | 27433447 |
| expression of two novel β-glucosidases from chaetomium atrobrunneum in trichoderma reesei and characterization of the heterologous protein products. | two novel gh3 family thermostable β-glucosidases from the filamentous fungus chaetomium atrobrunneum (cel3a and cel3b) were expressed in trichoderma reesei, purified by two-step ion exchange chromatography, and characterized. both enzymes were active over a wide range of ph as compared to neurospora crassa β-glucosidase gh3-3, which was also expressed in t. reesei and purified. the optimum temperature of both c. atrobrunneum enzymes was around 60 °c at ph 5, and both enzymes had better thermal a ... | 2016 | 27714589 |
| biohydrogen production from hydrolysates of selected tropical biomass wastes with clostridium butyricum. | biohydrogen production has received widespread attention from researchers in industry and academic fields. response surface methodology (rsm) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with clostridium butyrium, and achieved the highest production rate and yield of hydrogen. highest h2 yield of 2.02 mol h2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °c, while the composition of medium was (g/l): 15.66 glucose, 6.0 ... | 2016 | 27251222 |
| production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered escherichia coli strains. | in this study, the lactogenic escherichia coli strain ju15 was used and modified to produce d-lactate (d-la) from plant hydrolysates with a minimal nutrient addition in ph controlled fermenters. results showed that strain ju15 produces d-la with high yield and productivity in laboratory simulated hydrolysate media and actual sugar cane bagasse hemicellulosic hydrolysate. strain ju15 showed sequential carbon source utilization and acetic acid production. the l-lactic and acetic acid production pa ... | 2016 | 27573474 |
| effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (s)-4-chloro-3-hydroxybutanoate by whole cells of e. coli cczu-k14. | in this study, sugarcane bagasse (sb) was pretreated with combination pretreatment (e.g., sequential koh extraction and ionic liquid soaking, sequential koh extraction and fenton soaking, or sequential koh extraction and glycerol soaking). after the enzymatic hydrolysis of pretreated sbs, it was found that all these three concentrated hydrolyzates could be used for the asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate (cobe) into ethyl (s)-4-chloro-3-hydroxybutanoate [(s)-chbe]. compared ... | 2016 | 27060248 |
| activated carbon from pyrolysed sugarcane bagasse: silver nanoparticle modification and ecotoxicity assessment. | activated carbon from pyrolysed sugarcane bagasse (acpb) presented pore size ranges from 1.0 to 3.5nm, and surface area between 1200 and 1400m(2)g(-1) that is higher than commonly observed to commercial activated carbon. the acpb material was successfully loaded with of silver nanoparticles with diameter around 35nm (0.81wt.%). x-ray photoelectron spectroscopy (xps) analyses showed that the material surface contains metallic/ag(0) (93.60wt.%) and ionic/ag(+) states (6.40wt.%). the adsorption cap ... | 2016 | 27039274 |
| purification and characterization of recombinant sugarcane sucrose phosphate synthase expressed in e. coli and insect sf9 cells: an importance of the n-terminal domain for an allosteric regulatory property. | sucrose phosphate synthase (sps) catalyses the transfer of glycosyl group of uridine diphosphate glucose to fructose-6-phosphate to form sucrose-6-phosphate. plant sps plays a key role in photosynthetic carbon metabolisms, which activity is modulated by an allosteric activator glucose-6-phosphate (g6p). we produced recombinant sugarcane sps using escherichia coli and sf9 insect cells to investigate its structure-function relationship. when expressed in e. coli, two forms of sps with different si ... | 2016 | 26826371 |
| plasmidic expression of nema and yafc* increased resistance of ethanologenic escherichia coli ly180 to nonvolatile side products from dilute acid treatment of sugarcane bagasse and artificial hydrolysate. | hydrolysate-resistant escherichia coli sl100 was previously isolated from ethanologenic ly180 after sequential transfers in am1 medium containing a dilute acid hydrolysate of sugarcane bagasse and was used as a source of resistance genes. many genes that affect tolerance to furfural, the most abundant inhibitor, have been described previously. to identify genes associated with inhibitors other than furfural, plasmid clones were selected in an artificial hydrolysate that had been treated with a v ... | 2016 | 26826228 |
| de novo analysis of the transcriptome of pratylenchus zeae to identify transcripts for proteins required for structural integrity, sensation, locomotion and parasitism. | the root lesion nematode pratylenchus zeae, a migratory endoparasite, is an economically important pest of major crop plants (e.g. cereals, sugarcane). it enters host roots, migrates through root tissues and feeds from cortical cells, and defends itself against biotic and abiotic stresses in the soil and in host tissues. we report de novo sequencing of the p. zeae transcriptome using 454 flx, and the identification of putative transcripts encoding proteins required for movement, response to stim ... | 2016 | 26292651 |
| description and phylogenetic placement of beauveria hoplocheli sp. nov. used in the biological control of the sugarcane white grub, hoplochelus marginalis, on reunion island. | on reunion island successful biological control of the sugarcane white grub hoplochelus marginalis fairmaire (coleoptera: melolonthidae) has been conducted for decades with strains from the entomopathogenic fungal genus beauveria (ascomycota: hypocreales). a study based on morphological characters combined with a multisequence phylogenetic analysis of genes that encode the translation elongation factor 1-alpha (tef1), rna polymerase ii largest subunit (rpb1), rna polymerase ii second largest sub ... | 2016 | 26297783 |
| proteomic profile of hemolymph and detection of induced antimicrobial peptides in response to microbial challenge in diatraea saccharalis (lepidoptera: crambidae). | insects are organisms extremely well adapted to diverse habitats, primarily due to their innate immune system, which provides them with a range of cellular and humoral responses against microorganisms. lepidoptera hemolymph proteins involved in humoral responses are well known; however, there is a lack of knowledge about the sugarcane borer diatraea saccharalis. in this present work, the hemolymph proteins of this pest insect were studied by applying proteomic methodologies. two-dimensional elec ... | 2016 | 27012208 |
| xylan-specific carbohydrate-binding module belonging to family 6 enhances the catalytic performance of a gh11 endo-xylanase. | xylanases catalyze the hydrolysis of β-1,4-linked xylosyl moieties from xylan chains, one of the most abundant hemicellulosic polysaccharides found in plant cell walls. these enzymes can exist either as single catalytic domains or as modular proteins composed of one or more carbohydrate-binding modules (cbms) appended to the catalytic core. however, the molecular mechanisms governing the synergistic effects between catalytic domains and their cbms are not fully understood. thus, the goal of this ... | 2016 | 26923808 |
| engineering of bacillus subtilis for the production of 2,3-butanediol from sugarcane molasses. | 2,3-butanediol is known to be a platform chemical with several potential industrial applications. sustainable industrial scale production can be attained by using a sugarcane molasses based fermentation process using bacillus subtilis. however, the accumulation of acetoin needs to be reduced to improve process efficiency. in this work, b. subtilis was genetically modified in order to increase the yield of 2,3-butanediol. metabolic engineering strategies such as cofactor engineering and overexpre ... | 2016 | 26825987 |
| gh53 endo-beta-1,4-galactanase from a newly isolated bacillus licheniformis cbmai 1609 as an enzymatic cocktail supplement for biomass saccharification. | galactanases (endo-β-1,4-galactanases-ec 3.2.1.89) catalyze the hydrolysis of β-1,4 galactosidic bonds in arabinogalactan and galactan side chains found in type i rhamnogalacturan. the aim of this work was to understand the catalytic function, biophysical properties, and use of a recombinant gh53 endo-beta-1,4-galactanase for commercial cocktail supplementation. the nucleotide sequence of the endo-β-1,4-galactanase from bacillus licheniformis cbmai 1609 (bl1609gal) was cloned and expressed in es ... | 2016 | 26879978 |
| an entomopathogenic bacterium strain, bacillus thuringiensis, as a biological control agent against the red palm weevil, rhynchophorus ferrugineus (coleoptera: curculionidae). | the red palm weevil (rpw), rhynchophorus ferrugineus, is an invasive wood-boring insect that damages palms and sugarcane. bacillus thuringiensis (bt) is an entomopathogenic bacterium which has been modified into various strains and widely used in pest management. the aim of this study was to evaluate the susceptibility of rpw to the ha strain of bt. | 2016 | 27862867 |
| possibly similar genetic basis of resistance to bacillus thuringiensis cry1ab protein in 3 resistant colonies of the sugarcane borer collected from louisiana, usa. | the sugarcane borer, diatraea saccharalis (f.), is a major maize borer pest and a target of transgenic maize expressing bacillus thuringiensis (bt) proteins in south america and the mid-southern region of the united states. evolution of resistance in target pest populations is a great threat to the long-term efficacy of bt crops. in this study, we compared the genetic basis of resistance to cry1ab protein in 3 resistant colonies of sugarcane borer established from field populations in louisiana, ... | 2016 | 27860288 |
| reproductive isolation between two populations of diatraea saccharalis (f.) (lepidoptera: crambidae) from different host plant species and regions in argentina. | the sugarcane borer, diatraea saccharalis (f.), has a widespread distribution throughout the western hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. the use of bacillus thuringiensis (bt) corn has been the primary tool for managing this species in corn fields. sugarcane borer control has been recently threatened by observations of susceptibility and/or resistance to certain varieties of bt corn and the protein used in many newer varieties. this has led t ... | 2016 | 27112423 |
| production of cellulase from aspergillus terreus ms105 on crude and commercially purified substrates. | aspergillus terreus ms105 was originally isolated from soil and screened for cellulase production in the presence of various carbon sources including carboxymethyl cellulose (cmc), avicel, sigmacell, filter-paper and salicin. cmc induced the production of endoglucanase (eg) and filter-paperase while the levels of β-glucosidase (bgl) were increased when salicin was present in the medium. nature of production medium influenced the duration of lag- and log-phase of the growth, rate of fungal dry-ma ... | 2016 | 28330173 |
| cloning, expression, and purification of xylanase gene from bacillus licheniformis for use in saccharification of plant biomass. | the xylanase gene (xyna) of bacillus licheniformis 9945a was cloned and expressed in escherichia coli bl21(de3) using pet-22b(+) as an expression vector. the recombinant xylanase enzyme was purified by ammonium sulfate precipitation, followed by single-step immobilized metal ion affinity chromatography with a 57.58-fold purification having 138.2 u/mg specific activity and recovery of 70.08 %. molecular weight of the purified xylanase, 23 kda, was determined by sodium dodecyl sulfate polyacrylami ... | 2016 | 26438315 |
| production of l(+)-lactic acid from acid pretreated sugarcane bagasse using bacillus coagulans dsm2314 in a simultaneous saccharification and fermentation strategy. | sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. in our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail gc220 and fermented by the moderate thermophilic bacterium bacillus coagulans dsm2314. saccharification and fermentation were performed simultaneously (ssf), adding acid-pretreated bagasse either in one batch or in two stages. ssf was performed at low enzyme do ... | 2016 | 27872661 |
| construction of individual, fused, and co-expressed proteins of endoglucanase and β-glucosidase for hydrolyzing sugarcane bagasse. | at least a combination of endoglucanase (eglii) and β-glucosidase (bglz) is required for hydrolyzing crystalline cellulose. to understand the catalytic efficiency of combination enzymes for converting biomass to sugars, eglii and bglz were constructed in the form of individual, fused as well as co-expression proteins, and their activities for hydrolyzing sugarcane bagasse were evaluated. the genes, eglii isolated from bacillus amyloliquefaciens psm3.1 earlier and bglz from b. amyloliquefaciens a ... | 2016 | 24598011 |
| valorization of agro-industrial wastes towards the production of rhamnolipids. | in this work, oil mill wastewater (omw), a residue generated during olive oil extraction, was evaluated as an inducer of rhamnolipid production. using a medium containing as sole ingredients corn steep liquor (10%, v/v), sugarcane molasses (10%, w/v) and omw (25%, v/v), pseudomonas aeruginosa #112 produced 4.5 and 5.1g of rhamnolipid per liter in flasks and reactor, respectively, with critical micelle concentrations as low as 13mg/l. furthermore, in the medium supplemented with omw, a higher pro ... | 2016 | 27092993 |
| characterization of a thermotolerant phytase produced by rhizopus microsporus var. microsporus biofilm on an inert support using sugarcane bagasse as carbon source. | the rhizopus microsporus var. microsporus biofilm was able to produce increased levels of an extracellular thermotolerant phytase using polyethylene and viscose as an inert support in both modified nbrip medium and modified khanna medium containing sugarcane bagasse as the carbon source. the enzyme production was strictly regulated by the phosphorus content with optimal production at 0.5 mm of sodium phytate and kh2po4. the extracellular phytase, rmphy1, was purified 4.18-fold with 4.78 % recove ... | 2016 | 26906117 |
| a biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse. | in this study, a co-production of two high value-added products, glucose and xylooligosaccharides (xos), was investigated by utilizing sugarcane bagasse (sb) within a multi-product bio-refinery framework optimized by box-behnken design-based response surface methodology. the developed process resulted in a maximum cellulose conversion of xylan-removed sb, 98.69±1.30%, and a maximum extracted sb xylan conversion into xos (xylobiose and xylotriose) of 57.36±0.79% that was the highest sb xylan conv ... | 2016 | 26773956 |
| synergistic infection of two viruses mcmv and scmv increases the accumulations of both mcmv and mcmv-derived sirnas in maize. | the co-infection of maize chlorotic mottle virus (mcmv) and sugarcane mosaic virus (scmv) can cause maize lethal necrosis. however, the mechanism underlying the synergistic interaction between these two viruses remains elusive. in this study, we found that the co-infection of mcmv and scmv increased the accumulation of mcmv. moreover, the profiles of virus-derived sirnas (vsirnas) from mcmv and scmv in single- and co-infected maize plants were obtained by high-throughput sequencing. our data sho ... | 2016 | 26864602 |
| belowground ecology of scarabs feeding on grass roots: current knowledge and future directions for management in australasia. | many scarab beetles spend the majority of their lives belowground as larvae, feeding on grass roots. many of these larvae are significant pests, causing damage to crops and grasslands. damage by larvae of the greyback cane beetle (dermolepida albohirtum), for example, can cause financial losses of up to au$40 million annually to the australian sugarcane industry. we review the ecology of some scarab larvae in australasia, focusing on three subfamilies; dynastinae, rutelinae, and melolonthinae, c ... | 2016 | 27047506 |
| a strain of meyerozyma guilliermondii isolated from sugarcane juice is able to grow and ferment pentoses in synthetic and bagasse hydrolysate media. | the search for new microbial strains that are able to withstand inhibitors released from hemicellulosic hydrolysis and are also still able to convert sugars in ethanol/xylitol is highly desirable. a yeast strain isolated from sugarcane juice and identified as meyerozyma guilliermondii was evaluated for the ability to grow and ferment pentoses in synthetic media and in sugarcane bagasse hydrolysate. the yeast grew in xylose, arabinose and glucose at the same rate at an initial medium ph of 5.5. a ... | 2016 | 27038950 |
| saccharification of ozonated sugarcane bagasse using enzymes from myceliophthora thermophila jcp 1-4 for sugars release and ethanol production. | the saccharification of ozonated sugarcane bagasse (scb) by enzymes from myceliophthora thermophila jcp 1-4 was studied. fungal enzymes provided slightly higher sugar release than commercial enzymes, working at 50°c. sugar release increased with temperature increase. kinetic studies showed remarkable glucose release (4.99 g/l, 3%w/w dry matter) at 60°c, 8 h of hydrolysis, using an enzyme load of 10 fpu (filter paper unit). fpase and β-glucosidase activities increased during saccharification (284 ... | 2016 | 26773948 |
| optimization and characterization of pha from isolate pannonibacter phragmitetus erc8 using glycerol waste. | polyhydroxyalkanoates (phas) have been considered as a good alternative for petrochemical based polymers due to its biodegradability. however, a high production cost limits their acceptance in industries. in present work, efforts have been made to optimize the production of pha by pannonibacter phragmitetus erc8 using glycerol waste as a sole carbon source, with enhanced polymer production in a cost effective way. to check the possibility of growth and polymer accumulation potential of p. phragm ... | 2016 | 26851207 |
| draft genome sequence of leifsonia xyli subsp. xyli strain gdw1. | here, we report the draft genome sequence of leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of badila sugarcane located at the guangdong key laboratory for crops genetic improvement (guanzhou, china), that causes ratoon stunting disease of sugarcane. the de novo genome of leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a g+c content of 67.68%, and contained 2.6 mb bp and 2,838 coding sequences. | 2016 | 27795270 |
| rapid and quantitative detection of leifsonia xyli subsp. xyli in sugarcane stalk juice using a real-time fluorescent (taqman) pcr assay. | ratoon stunting disease (rsd) of sugarcane, one of the most important diseases seriously affecting the productivity of sugarcane crops, was caused by the bacterial agent leifsonia xyli subsp. xyli (lxx). a taqman probe-based real-time quantitative polymerase chain reaction (qpcr) assay was established in this study for the quantification of lxx detection in sugarcane stalk juice. a pair of pcr primers (pat1-qf/pat1-qr) and a fluorogenic probe (pat1-qp) targeting the part1 gene of lxx were used f ... | 2016 | 27725937 |
| fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria. | this study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (lab) isolated from sugarcane grown in a brazil on the quality of corn silage. | 2016 | 26496890 |
| screening of yeasts for selection of potential strains and their utilization for in situ microbial detoxification (ismd) of sugarcane bagasse hemicellulosic hydrolysate. | many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. the conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. hence, it is imperative to explore biological methods to overcome the inhibitors by ... | 2016 | 27570309 |
| effect of ozonolysis pretreatment parameters on the sugar release, ozone consumption and ethanol production from sugarcane bagasse. | a l9(3)(4) orthogonal array (oa) experimental design was applied to study the four parameters considered most important in the ozonolysis pretreatment (moisture content, ozone concentration, ozone/oxygen flow and particle size) on ethanol production from sugarcane bagasse (scb). statistical analysis highlighted ozone concentration as the highest influence parameter on reaction time and sugars release after enzymatic hydrolysis. the increase on reaction time when decreasing the ozone/oxygen flow ... | 2016 | 27132222 |
| distribution of dekkera bruxellensis in a sugarcane-based fuel ethanol fermentation plant. | we investigated the presence of the yeast dekkera bruxellensis in samples collected at three points surrounding the industrial alcoholic fermentation plants of two distilleries where there are often cases of contamination caused by this yeast: this involved sugar cane wash water, feeding sugar cane juice and vinasse from the treatment pond. total yeast was isolated in wln medium with bromocresol green and cycloheximide and further selected on the basis of its ability to grow in synthetic medium ... | 2016 | 26928357 |
| enhancement of penicillium echinulatum glycoside hydrolase enzyme complex. | the enhancement of enzyme complex produced by penicillium echinulatum grown in several culture media components (bagasse sugarcane pretreated by various methods, soybean meal, wheat bran, sucrose, and yeast extract) was studied to increment fpase, xylanase, pectinase, and β-glucosidase enzyme activities. the present results indicated that culture media composed with 10 g/l of the various bagasse pretreatment methods did not have any substantial influence with respect to the fpase, xylanase, and ... | 2016 | 26922416 |
| development of flocculent saccharomyces cerevisiae strain gyk-10 for the selective fermentation of glucose/fructose in sugar mills. | advances in glucose/fructose-selective ethanol production have successfully enhanced raw sugar extraction from sugarcane juice by converting inhibitory substances (i.e., glucose/fructose) into ethanol, which is removed by subsequent operations in cane sugar mills. however, the commercial implementation of this breakthrough process in existing cane sugar mills requires a yeast strain that (i) can be used in food production processes, (ii) exhibits stable saccharometabolic selectivity, and (iii) c ... | 2016 | 26811219 |
| sucrose and saccharomyces cerevisiae: a relationship most sweet. | sucrose is an abundant, readily available and inexpensive substrate for industrial biotechnology processes and its use is demonstrated with much success in the production of fuel ethanol in brazil. saccharomyces cerevisiae, which naturally evolved to efficiently consume sugars such as sucrose, is one of the most important cell factories due to its robustness, stress tolerance, genetic accessibility, simple nutrient requirements and long history as an industrial workhorse. this minireview is focu ... | 2016 | 26658003 |
| biochemical conversion of sugarcane straw hemicellulosic hydrolyzate supplemented with co-substrates for xylitol production. | biotechnological production of xylitol is an attractive route to add value to a sugarcane biorefinery, through utilization of the hemicellulosic fraction of sugarcane straw, whose availability is increasing in brazil. herein, supplementation of the sugarcane straw hemicellulosic hydrolyzate (xylose 57gl(-1)) with maltose, sucrose, cellobiose or glycerol was proposed, and their effect as co-substrates on xylitol production by candida guilliermondii fti 20037 was studied. sucrose (10gl(-1)) and gl ... | 2016 | 26615771 |
| hemicellulosic ethanol production by immobilized wild brazilian yeast scheffersomyces shehatae ufmg-hm 52.2: effects of cell concentration and stirring rate. | the use of sugarcane bagasse hemicellulosic hydrolysates presents an interesting alternative to second generation (2g) ethanol production. techniques to enhance the fermentation process, e.g., the use of immobilized cells, is one of the key factors for efficient production. here, the effect of two important parameters (cell concentration in immobilized system and stirring rate) on the 2g ethanol production using the wild brazilian yeast s. shehatae ufmg-hm 52.2 immobilized in calcium alginate ma ... | 2016 | 26507335 |
| mixed substrate fermentation for enhanced phytase production by thermophilic mould sporotrichum thermophile and its application in beneficiation of poultry feed. | the optimum values of the critical variables determined by the central composite design of response surface methodology (rsm) for maximum phytase production (1881.26 u g(-1) dry mouldy residue (dmr)) by sporotrichum thermophile are 2.5 % tween 80, 1.0 % yeast extract and 48 h of incubation period. phytase production in the mixed substrate (sugarcane bagasse and wheat bran) fermentation enhanced 11.6-fold over the initial production as a consequence of optimization. phytase titres are sustainable ... | 2016 | 26433602 |
| mechanistic study on ultrasound assisted pretreatment of sugarcane bagasse using metal salt with hydrogen peroxide for bioethanol production. | this study presents the ultrasound assisted pretreatment of sugarcane bagasse (scb) using metal salt with hydrogen peroxide for bioethanol production. among the different metal salts used, maximum holocellulose recovery and delignification were achieved with ultrasound assisted titanium dioxide (tio2) pretreatment (uatp) system. at optimum conditions (1% h2o2, 4 g scb dosage, 60 min sonication time, 2:100 m ratio of metal salt and h2o2, 75°c, 50% ultrasound amplitude and 70% ultrasound duty cycl ... | 2016 | 26384901 |
| development of a novel sequential pretreatment strategy for the production of bioethanol from sugarcane trash. | a novel sequential pretreatment strategy using biodiesel industry generated waste glycerol assisted transition metal and alkali pretreatment of sugarcane trash were developed for the production of bioethanol. various process parameters affecting pretreatment as well as hydrolysis were optimized by adopting a taguchi design. this novel method was found to be superior when compared to conventional pretreatment strategies like acid and alkali in removing hemicelluloses and lignin and the hydrolyzat ... | 2016 | 26318846 |
| improvement production of hyaluronic acid by streptococcus zooepidemicus in sugarcane molasses. | microbial hyaluronic acid (ha) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. in this context, to obtain an economically competitive ha production by streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. the highest ha production by s. zooepidemicus atcc 39920 achieved was 2.825 g. l(-1) in a 4.5 l bioreactor with controlled ph (8.0) and medium containing molasses (85.3 ... | 2016 | 27900664 |
| use of sugarcane molasses by pycnoporus sanguineus for the production of laccase for dye decolorization. | pycnoporus sanguineus is a white-rot basidiomycete that produces laccase as the only oxidoreductase; enzyme synthesis depends on cultivation variables, and fungal species and strain. laccases have wide substrate specificity, oxidize a broad range of compounds, and show potential for use in dye decolorization. we evaluated laccase production in a recently isolated strain of p. sanguineus cultivated with sugarcane molasses as the only carbon source, and urea or yeast extract as the nitrogen source ... | 2016 | 27813609 |
| magnesium ions in yeast: setting free the metabolism from glucose catabolite repression. | in a recent work we showed that magnesium (mg(ii)) plays an important role in industrial ethanol production, overcoming the negative effect of the excess of minerals, particularly copper, present in sugarcane juice, with a consequent increase in ethanol yield. this cation has been reported to be involved in several steps of yeast metabolism, acting mainly as a co-factor of several enzymes of fermentation metabolism and protecting yeast cells from stressful conditions. however, despite many physi ... | 2016 | 27714092 |
| sugarcane transcriptome analysis in response to infection caused by acidovorax avenae subsp. avenae. | sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. crop productivity is negatively affected by acidovorax avenae subsp avenae (aaa), which causes the red stripe disease. little is known about the molecular mechanisms triggered in response to the infection. we have investigated the molecular mechanism activated in sugarcane using a rna-seq approach. we have produced a de novo transcriptome assembly (tr7) from sugarcane rna-seq libraries submitted to drought an ... | 2016 | 27936012 |
| comparative proteomics reveals that central metabolism changes are associated with resistance against sporisorium scitamineum in sugarcane. | sugarcane smut, which is caused by sporisorium scitamineum, has been threatening global sugarcane production. breeding smut resistant sugarcane varieties has been proven to be the most effective method of controlling this particular disease. however, a lack of genome information of sugarcane has hindered the development of genome-assisted resistance breeding programs. furthermore, the molecular basis of sugarcane response to s. scitamineum infection at the proteome level was incomplete and combi ... | 2016 | 27733120 |
| de novo analysis of transcriptome reveals genes associated with leaf abscission in sugarcane (saccharum officinarum l.). | sugarcane (saccharum officinarum l.) is an important sugar crop which belongs to the grass family and can be used for fuel ethanol production. the growing demands for sugar and biofuel is asking for breeding a sugarcane variety that can shed their leaves during the maturity time due to the increasing cost on sugarcane harvest. | 2016 | 26946183 |
| isolation and characterization of scglud2, a new sugarcane beta-1,3-glucanase d family gene induced by sporisorium scitamineum, aba, h2o2, nacl, and cdcl2 stresses. | beta-1,3-glucanases (ec 3.2.1.39), commonly known as pathogenesis-related (pr) proteins, play an important role not only in plant defense against fungal pathogens but also in plant physiological and developmental processes. however, only a limited number of sugarcane beta-1,3-glucanase genes have been isolated. in the present study, we identified and characterized a new beta-1,3-glucanase gene scglud2 (genbank acc no. kf664181) from sugarcane. an x8 domain was present at the c terminal region of ... | 2016 | 27642288 |
| rnaseq transcriptional profiling following whip development in sugarcane smut disease. | sugarcane smut disease is caused by the biotrophic fungus sporisorium scitamineum. the disease is characterized by the development of a whip-like structure from the primary meristems, where billions of teliospores are produced. sugarcane smut also causes tillering and low sucrose and high fiber contents, reducing cane productivity. we investigated the biological events contributing to disease symptoms in a smut intermediate-resistant sugarcane genotype by examining the transcriptional profiles ( ... | 2016 | 27583836 |
| crosstalk between sugarcane and a plant-growth promoting burkholderia species. | bacterial species in the plant-beneficial-environmental clade of burkholderia represent a substantial component of rhizosphere microbes in many plant species. to better understand the molecular mechanisms of the interaction, we combined functional studies with high-resolution dual transcriptome analysis of sugarcane and root-associated diazotrophic burkholderia strain q208. we show that burkholderia q208 forms a biofilm at the root surface and suppresses the virulence factors that typically trig ... | 2016 | 27869215 |
| bacterial abilities and adaptation toward the rhizosphere colonization. | the rhizosphere harbors one of the most complex, diverse, and active plant-associated microbial communities. this community can be recruited by the plant host to either supply it with nutrients or to help in the survival under stressful conditions. although selection for the rhizosphere community is evident, the specific bacterial traits that make them able to colonize this environment are still poorly understood. thus, here we used a combination of community level physiological profile (clpp) a ... | 2016 | 27610108 |
| relationship of soil properties and sugarcane yields to red stripe in louisiana. | symptoms of red stripe disease caused by acidovorax avenae subsp. avenae in louisiana between 1985 and 2010 were limited to the leaf stripe form, which caused no apparent yield loss. during 2010, the more severe top rot form was observed, and a study was initiated to investigate the distribution of red stripe in the field and determine its effects on cane and sugar yields. soil properties data, red stripe incidence, and sugarcane yields were all highly variable and were not randomly distributed ... | 2016 | 27003508 |
| erratum for fontana et al., genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | 2016 | 26988059 | |
| genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | red stripe of sugarcane in argentina is a bacterial disease caused by acidovorax avenae. the genome sequence from the first isolate of this bacterium in argentina is presented here. the draft genome of the a. avenae t10_61 strain contains 5,646,552 bp and has a g+c content of 68.6 mol%. | 2016 | 26847889 |
| advances in agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops. | steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene tran ... | 2016 | 26660352 |
| emerging technologies for the production of renewable liquid transport fuels from biomass sources enriched in plant cell walls. | plant cell walls are composed predominantly of cellulose, a range of non-cellulosic polysaccharides and lignin. the walls account for a large proportion not only of crop residues such as wheat straw and sugarcane bagasse, but also of residues of the timber industry and specialist grasses and other plants being grown specifically for biofuel production. the polysaccharide components of plant cell walls have long been recognized as an extraordinarily large source of fermentable sugars that might b ... | 2016 | 28018390 |
| characterization of three plant biomass-degrading microbial consortia by metagenomics- and metasecretomics-based approaches. | the selection of microbes by enrichment on plant biomass has been proposed as an efficient way to develop new strategies for lignocellulose saccharification. here, we report an in-depth analysis of soil-derived microbial consortia that were trained to degrade once-used wheat straw (ws1-m), switchgrass (sg-m) and corn stover (cs-m) under aerobic and mesophilic conditions. molecular fingerprintings, bacterial 16s ribosomal rna (rrna) gene amplicon sequencing and metagenomic analyses showed that th ... | 2016 | 27418359 |
| identification of genes involved in indole-3-acetic acid biosynthesis by gluconacetobacter diazotrophicus pal5 strain using transposon mutagenesis. | gluconacetobacter diazotrophicus is a beneficial nitrogen-fixing endophyte found in association with sugarcane plants and other important crops. beneficial effects of g. diazotrophicus on sugarcane growth and productivity have been attributed to biological nitrogen fixation process and production of phytohormones especially indole-3-acetic acid (iaa); however, information about the biosynthesis and function of iaa in g. diazotrophicus is still scarce. therefore, the aim of this work was to ident ... | 2016 | 27774087 |
| ethanol production in brazil: a bridge between science and industry. | in the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in brazil. these contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. better control of fermentation conditions can ... | 2016 | 27818090 |
| influence of vinasse application in the structure and composition of the bacterial community of the soil under sugarcane cultivation. | although the use of vinasse as a waste helps replenish soil nutrients and improves the quality of the sugarcane crop, it is known that vinasse residues alter the diversity of bacteria naturally present in the soil. the actual impacts of vinasse application on the selection of bacterial taxa are not understood because no studies have addressed this phenomenon directly. analysis of 16s rrna gene clone sequences from four soil types showed that the soil planted with sugarcane and fertilized with vi ... | 2016 | 27528875 |
| essential genes for in vitro growth of the endophyte herbaspirillum seropedicae smr1 as revealed by transposon insertion site sequencing. | the interior of plants contains microorganisms (referred to as endophytes) that are distinct from those present at the root surface or in the surrounding soil. herbaspirillum seropedicae strain smr1, belonging to the betaproteobacteria, is an endophyte that colonizes crops, including rice, maize, sugarcane, and sorghum. different approaches have revealed genes and pathways regulated during the interactions of h. seropedicae with its plant hosts. however, functional genomic analysis of transposon ... | 2016 | 27590816 |
| mapping n-linked glycosylation of carbohydrate-active enzymes in the secretome of aspergillus nidulans grown on lignocellulose. | the genus aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (cazymes) that characterize their saprophyte lifestyle. aspergillus has the capacity to perform post-translational modifications (ptm), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins. in this study, the n-linked glycosylation of cazymes identified in the secretome of asperg ... | 2016 | 27508003 |
| rnaseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose. | sugarcane is one of the world's most profitable crops. waste steam-exploded sugarcane bagasse (seb) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. in nature, fungi seldom exist as planktonic cells, similar to those found in the nutrient-rich environment created within an industrial fermenter. instead, fungi predominantly form biofilms that allow them to thrive in hostile environments. | 2016 | 27437031 |
| characterization and biotechnological application of recombinant xylanases from aspergillus nidulans. | two xylanases from aspergillus nidulans, xlnb and xlnc, were expressed in pichia pastoris, purified and characterized. xlnb and xlnc achieved maximal activities at 60°c and ph 7.5 and at 50°c and ph 6.0, respectively. xlnb showed to be very thermostable by maintaining 50% of its original activity after 49h incubated at 50°c. xlnb had its highest activity against wheat arabinoxylan while xlnc had the best activity against beechwood xylan. both enzymes were completely inhibited by sds and hgcl2. x ... | 2016 | 27235731 |
| co-cultivation of aspergillus nidulans recombinant strains produces an enzymatic cocktail as alternative to alkaline sugarcane bagasse pretreatment. | plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. a clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. however, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. replacing these methods with co-culti ... | 2016 | 27199917 |