Publications

accessibility of phospholipids in the chromaffin granule membrane.	1. the accessibility of phospholipids in the membrane of the adrenomedullary storage vesicles (chromaffin granules) has been studied. 2. the reaction of 2,4,6-trinitrobenzenesulphonic acid with both intact granules and their ghosts, results in the labelling of 70% of the phosphatidylethanolamine. 3. the action of phospholipase a2 (from bee venom), phospholipase c (from bacillus cereus) and sphingomyelinase c (from staphylococcus aureus) on granules and their ghosts was followed as a function of ...	1978	102348
clavulanic acid inhibition of beta-lactamase i from bacillus cereus 569/h.	inactivation of beta-lactamase i by clavulanic acid was investigated. clavulanic acid induced inhibition of the enzyme was found to be progressive with time. benzylpenicillin provided protection against the adverse effects of the inhibitor initially, however, the enzyme was irreversibly inhibited in a progressive manner even in the presence of substrate. reaction of beta-lactamase i with clavulanic acid, in the presence of ampicillin, led to a very rapid inactivation of the enzyme.	1978	102630
inhibition by potassium ion of the pregerminative response to l-alanine of unactivated spores of bacillus cereus t.	the effect of potassium ion on l-alanine-inosine-induced germination of unactivated spores of bacillus cereus t was studied. unactivated spores germinated in 0.1 m sodium phosphate buffer (napb), but not 0.1 m potassium phosphate buffer (kpb), at ph 8.0 and at 30 c. inhibition of germination was also observed on incubation of unactivated spores in napb containing potassium chloride. previously it was demonstrated that germination of unactivated spores involves at least two steps, one induced by ...	1978	102904
[supplementary approach to the differentiation of the spore bacteria, bacillus subtilis and bacillus cereus].		1978	102905
case report: bacillus cereus food poisoning.		1978	103816
relationship of bacillus subtilis dna polymerase iii to bacteriophage pbs2-induced dna polymerase and to the replication of uracil-containing dna.	in vivo studies of pbs2 phage replication in a temperature-sensitive bacillus subtilis dna polymerase iii (pol iii) mutant and a temperature-resistant revertant of this mutant have suggested the possible involvement of pol iii in pbs2 dna synthesis. previous results with 6-(p-hydroxyphenylazo)-uracil (hpura), a specific inhibitor of pol iii and dna replication in uninfected cells, suggest that pol iii is not involved in phage dna replication, due to its resistance to this drug. experiments were ...	1978	104052
[enteropathogenicity of bacillus cereus strains].		1978	104365
hybridization analysis of restriction endonuclease dna fragments of bacillus cereus transcribed during spore outgrowth.	transcribing bacillus cereus dna was visualized by means of autoradiography of electrophoretically separated ecori restriction endonuclease dna fragments hybridizing 32p-labeled rna. hybridization of rna of dormant spores, vegetative cells, and outgrowing spores indicates the following. (i) a large fraction of the nonribosomal rna in dormant spores is transcribed at a limited number of regions on the bacterial chromosome. (ii) after induction of spore germination, transcription activity is not l ...	1978	96096
properties of bacillus cereus temperature-sensitive mutants altered in spore coat formation.	three conditional bacillus cereus mutants altered in the assembly or formation of spore coat layers were analyzed. they all grew as well as the wild type in an enriched or minimal medium but produced lysozyme and octanol-sensitive spores at the nonpermissive temperature (35 to 38 degrees c). the spores also germinated slowly when produced at 35 degrees c. temperature-shift experiments indicated that the defective protein or regulatory signal is expressed at the time of formation of the outer spo ...	1978	96097
[repeated microcycle in bacillus cereus].		1978	96318
the effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase c in bacillus cereus.	the effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase c (ec 3.1.4.3) has been studied in washed bacillus cereus cells resuspended in nutrient broth. in the absence of inhibitors, the synthesis showed a biphasic pattern. no synthesis of release of enzyme was found in the presence of chloramphenicol. when rifampicin was added, phospholipase c synthesis continued for 10-15 min. nalidixic acid, at concentrations which inhibited dna synthesis completely, permi ...	1978	96661
bacillus cereus-induced malabsorption in young mice.	following a single, oral dose of bacillus cereus (2 x 10(8) bacteria) in vitro intestinal absorption of d-glucose, d-galactose, l-arginine, l-histidine, l-ornithine and l-proline in young mice (aged 2--3 1/2 months) decreased. malabsorption of d-glucose was dose- and time-dependent. impaired absorption of d-glucose occurred throughtout the length of the small intestine, particularly distally. following hydrolysis of d-maltose at the brush border, d-glucose absorption in infected mice and that of ...	1978	97113
an improved electrophoretic method for identifying antibiotics with special reference to animal tissues and animal feeding stuffs.		1978	97257
preliminary x-ray crystallographic data on phospholipase c from bacillus cereus.		1978	97390
inhibition by ammonium ion of germination of unactivated spores of bacillus cereus t induced by l-alanine and inosine.	studies were carried out on the inhibitory effect of nh4+ on germination of spores of bacillus cereus t induced by l-alanine and inosine. kinetic analysis showed that nh4+ inhibited the germination competitively. its inhibitory effect was greater when the unactivated spores had been preincubated with l-alanine. nh4+ did not inhibit the response of unactivated spores to l-alanine during preincubation. these results suggest that l-alanine sensitizes the spores to the inhibitory effect of nh4+.	1978	97499
bacillus cereus endocarditis. a case report.	bacillus cereus may cause infective problems in compromised patients. no previous record of infective endocarditis due to this organism could be found. a 51-year-old white woman with b. cereus endocarditis after prosthetic mitral valve replacement is described. the problems of interpreting the significance of b. cereus bacteraemia, delayed diagnosis, and the inherent resistance of the organism are discussed.	1978	97794
[study of lysogeny in bacillus thuringiensis and b. cereus].	forty-eight strains of bacillus thuringiensis and 12 strains of b. cereus were treated with ultraviolet light and mitomycin c. the former agent was the more effective inducer. bacillus thuringiensis produces at least seven different phage particles with long, non-contractile tails. the frequencies of lysogeny and polylysogeny are 83 and 25% respectively. morphologically defective phages occur in 25% of strains, whereas five of them produce low molecular-weight bacteriocins. one strain of b. cere ...	1978	98223
effects of phenylglyoxal on growth & sporulation of bacillus cereus t.		1978	98431
formation and function of n-acetyloglucosamine-linked phosphoryl- and pyrophosphorylundecaprenols in membranes from bacillus cereus.	membranes from bacillus cereus ahu 1356 incorporated radioactivity from udp-n-acetyl[14c]glucosamine into three alkaline-stable and acid-labile lipids which were extracted into chloroform:methanol (2:1) and separated from each other by thin layer chromatography on silica gel plates. the major labeled lipid (lipid 1) and a minor one (lipid 2) were identified as n-actetylglucosaminyl phosphorylundecaprenol from several analytical criteria involving mass spectral data and from reversal of their for ...	1978	98528
two purine nucleoside phosphorylases in bacillus subtilis. purification and some properties of the adenosine-specific phosphorylase.	two purine nucleoside phosphorylases (purine-nucleoside:orthophosphate ribosyltransferase, ec 2.4.2.1) were purified from vegetative bacillus subtilis cells. one enzyme, inosine-guanosine phosphorylase, showed great similarity to the homologous enzyme of bacillus cereus. it appeared to be a tetramer of molecular weight 95 000. the other enzyme, adenosine phosphorylase, was specific for adenosine and deoxyadenosine. the molecular weight of the native enzyme was 153 000 +/- 10% and the molecular w ...	1978	99174
histidine residues of zinc ligands in beta-lactamase ii.	1. the zn(ii)-requiring beta-lactamase from bacillus cereus 569/h/9, which has two zinc-binding sites, was examined by 270 mhz 1h n.m.r. spectroscopy. resonances were assigned to five histidine residues. 2. resonances attributed to three of the histidine residues in the apoenzyme shift on the addition of one equivalent of zn(ii). 3. although these three histidine residues are free to titrate in the apoenzyme, none of them titrates over the ph range 6.0--9.0 in the mono-zinc enzyme. 4. the abilit ...	1978	33655
production of l-glutamic acid by a bacillus sp.	a strain of bacillus cereus var. mycoides isolated from burdwan soil produces l-glutamate in the medium. the strain is able to grow and produce in a synthetic medium but supplementation with casamino acid or yeast extract improves the yield. maintenance of ph of the fermentation medium near neutrality prolongs the active growth period and improves the yield. glucose and ammonium nitrate were found to be most suitable carbon and nitrogen sources, respectively. cane sugar molasses (as a substitute ...	1978	33882
the isolation and cultivation of a single spore using a sterile disposable petri dish and a micromanipulator.		1978	105218
deoxyribonucleic acid relatedness between bacillus anthracis, bacillus cereus and bacillus thuringiensis.		1978	105229
[information on phase contrast and differential interference contrast figures--a comparative study (author's transl)].		1978	105312
changes in ultraviolet resistance and photoproduct formation as early events in spore germination of bacillus cereus t.		1978	105364
[b. cereus count in meat and dairy food products].	studies were carried out to establish the contamination of some meat and dairy food products with b. cereus. a total of 48 heat-treated sausages (32 perishable and 16 durable) and 64 batches of pasteurized milk were sampled. it was found that 25 per cent of investigated sausage samples contained b. cereus. perishables proved to a considerable extent more frequently contaminated (33.3 per cent). the count of b. cereus in such products ranged from 10(1) to 10(3) per g. however, pasteurized milk wa ...	1978	105462
[serological properties of species of the genus bacillus].		1978	82901
studies on sphingomyelinase of bacillus cereus. i. purification and properties.	a sphingomyelinase was purified 980-fold with recovery of 25.6% from the culture broth of bacillus cereus, by (nh4)2so4 precipitation and chromatography on cm-sephadex, deae-cellulose and sephadex g-75. the purified preparation was free of lipase, protease and other phospholipases. the enzyme specifically hydrolyzed sphingomyelin to ceramide and phosphorylcholine. lysophosphatidylcholine was also attacked by the enzyme. the enzyme (mr = 24 000) was maximally active at ph 6-7. other properties of ...	1978	23854
effect of ph on the antimicrobial activity of some triphenylmethane dyes.	four common dyes were tested as inhibitors of four types of bacteria over the ph range 5.0-9.0. inhibition of the gram-negative types, salmonella anatum and enterobacter aerogenes, was markedly affected by the ph of the medium. these organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at ph 5.0 than at ph 9.0. above ph 7.0 brilliant green (bg) and malachite green (mg) were precipitated as their respective carbinols and lost their inhibitory properties with ...	1978	27297
interactions between macromolecular adjuvants and drugs. 11. the influence of macromolecules on the microbiologic activity of tetracycline antibiotics.		1978	27822
tissue sterility in uneviscerated carcasses.	sheep muscle tissue removed aseptically from control carcasses, from uneviscerated carcasses held at 20 degrees c for 24 h, and from carcasses of sheep subjected to stress before slaughter was examined for the presence of bacteria. all samples from a total of 68 carcasses were sterile. whole-body autoradiography of mouse carcasses showed that 14c-labeled fixed bacteria injected after death remained in the lumen of the intestine. live bacteria did not penetrate the mucosal surface until the tissu ...	1978	29564
the influence of high concentrations of carbon dioxide on the germination of bacterial spores.		1978	31348
effects of glucose, ph, and dissolved-oxygen tension on bacillus cereus growth and permeability factor production in batch culture.	the production of a bacillus cereus enterotoxin, measured as rabbit skin permeability factor (pf), in response to differences in glucose availability, ph, and dissolved oxygen tension was studied in a 1-liter batch fermentor system. glucose had to be present for toxigenesis to occur. in uncontrolled fermentation an increasing inhibition of pf production and growth occurred as ph dropped occurred below 6.5. optimum ph for toxigenesis was 7.0 to 7.5, and fermentations maintained at this level yiel ...	1979	32838
specific staining of bacilli by means of immuno-indian-ink method and immunofluorescent method.	the possibilities for identification of b. anthracis are investigated by means of the immuno-indian-ink method (iiim) and the immunofluorescent method (ifm) in their direct and indirect modifications. the specifity of sera against noncapsulated cells of b. anthrasis increases by their adsorption with antigenically related strains of b. cereus as their vegetative cells and spores are killed beforehand with performic acid. repeated uses of the bacillus suspension for adsorption is possible by trea ...	1979	87066
shielding of phospholipid vesicles from phospholipase c hydrolysis by alpha-lactalbumin adsorption [proceedings].		1979	92261
binding of exogenous dna by human lymphocytes and by their isolated plasma membranes.	the binding of labeled bacterial dna to human tonsil lymphocytes and to plasma membranes isolated from these cells involved macromolecules mainly located at the cell surface, since plasma membrane preparations showed properties in common with those of viable cells. dna binding to lymphocytes was a dissociable, saturable, time, temperature, ph and concentration dependent process. a double reciprocal plot of the data obtained from dna saturation curves gave an apparent dissociation constant of abo ...	1979	94969
bacillus cereus endogenous panophthalmitis.	a case of severe suppurative endogenous panophthalmitis caused by bacillus cereus resulted from intravenously administered medications. this is the first, to our knowledge, well-documented case of endogenous endophthalmitis associated with this organism. it is recommended that if on gram's stain of the anterior chamber fluid, gram-positive rods are seen, chloramphenicol should be administered in addition to penicillin because of the possibility of b cereus infection.	1979	105693
effect of purified phospholipases on the binding of tetrodotoxin to axon plasma membrane.	the role of phospholipids in the binding of [3h]tetrodotoxin to garfish olfactory nerve axon plasma membrane was studied by the use of purified phospholipases. treatment of the membranes with low concentrations of either phospholipase a2 (crotalus adamanteus and naja naja) or phospholipase c (bacillus cereus and clostridium perfringens) resulted in a marked reduction in tetrodotoxin binding activity. a 90% reduction in the activity occurred with about 45% hydrolysis of membrane phospholipids by ...	1979	39172
effect of trichloroacetic acid treatment on certain properties of spores of bacillus cereus t.	spores of bacillus cereus t treated with trichloroacetic acid (6.1--61.2 mm) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 n) and hydrochloric acid (0.27 n), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; an ...	1979	41162
an adenosine triphosphate dependent deoxyribonuclease with adenosine triphosphatase, activity from bacillus cereus.	an adenosine triphosphate-stimulated deoxyribonuclease was purified to about 4200 fold from bacillus cereus. the enzyme activity of the crude extract increased by a factor of about 5 after dialysis. one of the low molecular weight inhibitors of the crude extract was found to be inorganic phosphate. during enzyme purification two nucleases were identified. one of them was specific to denatured dna and the other one degraded both denatured dna and native dna. the activity towards native dna could ...	1979	42271
protein synthesizing systems from spores and vegetative cells of bacillus cereus.	a system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of bacillus cereus t. ribosomes of both types react similarly, showing a magnesium optimum of about 6mm and spermidine optima of about 5mm and 4mm for vegative and spore ribosomes, respectively. these lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mm ammonium ion. a comparison of the response of these ribosomes to ...	1979	42369
[studies of the purification of the exotoxin of bacillus cereus (author's transl)].	the exotoxins of bacillus cereus show haemolytic, lethal, phospholipase-c- and enterotoxigenic activities. the enterotoxigenic activity is regarded to be the factor which causes food poisoning in man. efforts to purify the b. cereus exotoxins by precipitation with ammonium sulphate and subsequent chromatography on sephadex-g-75 and biogel-p-60 columns were partially successfull. haemolysin and phospholipase-c could be separated by gel-chromatography, they demonstrated partial identity on gel-dif ...	1979	44788
properties and production characteristics of vomiting, diarrheal, and necrotizing toxins of bacillus cereus.	evidence is provided that the enterotoxin of bacillus cereus variously described in the literature as diarrheagenic toxin, diarrheal agent, fluid accumulation factor, vascular permeability factor, dermonecrotic toxin, and intestinonecrotic toxin is a single relatively unstable protein of molecular weight approximately 50,000 and isoelectric point of the order of 4.9. it is presumed to be the enterotoxin responsible for the diarrheal-type b. cereus food poisoning syndrome and it may also be the p ...	1979	104614
a novel glycosidase, an endo-glucosaminidase active on the cell wall peptidoglycan with n-unsubstituted glucosamine residues.		1979	104886
effects of local anesthetics on bacterial cells.	the membrane effects of chlorpromazine, nupercain, tetracain, and procain were studied using bacillus cereus, b. megaterium, b. subtilis, and streptococcus faecalis, protoplasts from s. faecalis, and isolated membranes from b. subtilis. chlorpromazin, nupercain, and tetracain produced characteristic micromorphological alterations after treatment for 5 to 30 min at ph 7.0 and 20 degrees c; the membrane staining pattern changed from asymmetric to symmetric, complex mesosome-like structures appeare ...	1979	104970
bacillus cereus endocarditis.		1979	105070
serious infections from bacillus sp.	serious infections caused by organisms of the genus bacillus developed in seven patients. five drug abusers had either endocarditis or osteomyelitis, one leukemic patient had necrotizing fasciitis, and one patient had a ventriculoatrial shunt infection with recurrent bacteremia. all patients recovered. experience with these cases reemphasizes the importance of not dismissing bacillus organisms as culture contaminants, especially when isolated from blood, body fluids, or closed-space infections.	1979	105158
interaction of phenosafranine with nucleic acids and model polyphosphates. iii. heterogeneity in phenosafranine interactions with dna base pairs.	fluorescence and circular dichroism spectral measurements, thermal denaturation studies and binding competition experiments with netropsin and actinomycin d were carried out in systems containing phenosafranine bound to dna's differing in base composition. the investigated properties exhibit a heterogeneity related to the content of a.t and g.c pairs in dna and to the nature of phenosafranine binding modes. at low level of saturation of binding sites (r less than 0.1) phenosafranine does not sho ...	1979	106900
severe clinical conditions associated with bacillus cereus and the apparent involvement of exotoxins.	twenty-one cases of infection with bacillus cereus are summarised. the histories supplied showed that at least 15 of these were associated with severe or potentially severe symptoms including two deaths. analysis of the production of exotoxins, including haemolysin and phospholipase, by these strains is given, and the relevance of these metabolites to the severity of the condition is discussed. three incidents of bovine mastitis resulting from b. cereus and involving three deaths are also includ ...	1979	107202
[inorganic ion transport in bacteria].		1979	107670
[infrared spectra of bacillus cereus cells].		1979	108522
[change in the constructive metabolism and ultrastructural organization of bacillus cereus cells under the influence of a specific autoregulatory factor].	it has been found for the first time that an increase in the concentration of a specific autoregulatory factor in the medium is responsible for the hypometabolic state of vegetative cells; no principal cellular biopolymers are synthesized at this state (dna, rna, proteins and phospholipids), and no cellular structures are degraded. when the culture reverses to vegetative growth observed in the same medium, synthesis of the biopolymers is intensified and changes are detected in the ultrastructura ...	1979	108528
catalytic and conformational properties of cross-linked derivatives of penicillinase.		1979	109124
a hospital food-borne outbreak of diarrhea caused by bacillus cereus: clinical, epidemiologic, and microbiologic studies.	an outbreak of diarrhea involving 28 patients occurred in two wards of a chronic disease hospital. the illness was characterized by abdominal cramps and watery diarrhea without vomiting or fever. an epidemiologic investigation suggested food-borne intoxication and incriminated turkey loaf served at the preceding evening meal as the source of the outbreak. bacillus cereus was isolated both from the stool of all 14 symptomatic patients who were cultured and from turkey loaf. no other enteropathoge ...	1979	109549
bacillus cereus panophthalmitis after intravenous heroin.	two healthy young black men developed panophthalmitis after intravenous heroin injections. bacillus cereus, considered to be a relatively noncommon pathogen for man, was found to be the causative agent as it was recovered from the anterior chamber and viterous cavity of both cases. the ocular findings were unilateral in each case, and neither patient had any sistemic involvement from the bacteremia. the onset of visual symptoms varied from 24 to 36 hours after the last intravenous injection with ...	1979	110208
purification of phospholipase c from bacillus cereus by hydrophobic chromatography on palmitoyl cellulose.	phospholipase c (phosphatidylcholine choline-phosphohydrolase, ec 3.1.4.e) from bacillus cereus (iam-1208) was adsorbed to palmitoyl cellulose from a crude enzyme solution at ph 5--9. the adsorption was not influenced by ionic strength up to 2 m nacl. the adsorbed enzyme was eluted almost completely by washing the cellulose with a suitable detergent, such as triton x-100, adekatol so-120, cation dt-205, or sodium deoxycholate. the enzyme was then purified by column chromatography on a palmitoyla ...	1979	110896
[bacteria of current interest. 4. new infections caused by known bacteria-- beta group streptococcus, haemophilus influenzae, aeromonas and bacillus infections].		1979	110966
infectious diarrhea.		1979	111270
[pathogenicity of aerobically sporulating microorganisms: bacillus cereus].	the pathogenicity of the metabolites of b. cereus was determined by bioassays with white mice, chick embryos, rabbits, and kittens. cell-free filtrates of the cultures of several strains of b. cereus were used for application; in the mice the application was done i. v., i. p. and p. o., in the chick embryos via the allantoic sac, in the kittens p. o. and i. p., in the rabbits via tied-up sections of the small intestine by the loop-test method. it was revealed that some strains of b. cereus produ ...	1979	111402
[structural changes in spores under high temperature exposure].	the electron microscopy of ultrathin sections of b. cereus spores showed that no lysis and destructive changes occurred in the main structural components of the spores when heated to 99 degrees c (in distilled water). by the time 99% of the population were destroyed, the spores seemed to preserve the exosporium of the sporoderm, the cortex and the sporoblast intact. even autoclaving at 120 degrees c for 15 min brought about no visible changes in the ultrastructure of the spores, though it killed ...	1979	111441
biochemical changes occurring during microcycle sporogenesis of bacillus cereus t.		1979	111675
inhibition of germination of bacillus cereus t spores by phenylglyoxal.	phenylgloxal at a concentration of 0.6 mm inhibited germination of bacillus cereus t spores as characterized by a decrease in absorbance, dipicolinic acid and loss in heat resistance in a chemically defined growth and sporulation medium. in a germination medium containing l-alanine and adenosine, phenylglyoxal inhibited decrease in absorbance and affected partial loss of viability. it is postulated that phenylglyoxal interacts with free amino groups of various enzymes or amino compounds present ...	1979	112015
[toxins and enzymes of several species of bacillus, especially of the b. cereus-thuringiensis group (author's transl)].	in the examined strains the production of following toxins or enzymes was determined by bioassay or by semiquantitative and routine diagnostical tests: delta-endotoxin, alpha-exotoxin, beta-exotoxin hemolysin, phospholipase c, proteinase. the production of delta-endotoxin (= a parasporal crystal toxic for several insects) is the only character in that b. thuringiensis differs from b. cereus. other biochemical features as production of so-called alpha-exotoxin (= soluble toxic protein), hemolysin ...	1979	112804
unfolding and refolding of phospholipase c from bacillus cereus in solutions of guanidinium chloride.	1. protein-fluorescence studies indicated that phospholipase c from bacillus cereus is denatured in solutions of guanidinium chloride. the denaturation was not thermodynamically reversible and followed biphasic kinetics. 2. guanidinium chloride solutions released the structural zn2+ from the enzyme and rendered all histidine residues chemically reactive. in the presence of free zn1+ the enzyme was much more resistant to denaturation. also, the addition for free zn2+ to the denatured enzyme induc ...	1979	113000
physiological studies of a temperature-sensitive sporulation mutant of bacillus cereus t.	growth of temperature-sensitive mutant bacillus cereus t js22-c occurred normally at the restrictive temperature (37 degrees c), but sporulation was blocked at stage 0. the production of extracellular and intracellular proteases and of alkaline phosphatase occurred at 37 degrees c, but the expression of a functional tricarboxylic acid cycle did not. at the permissive temperature (26 degrees c), the mutant sporulated at a slightly lower frequency (60%) and at a lower rate than the parent strain. ...	1979	113065
the effect of transition metal ions on the resistance of bacterial spores to hydrogen peroxide and to heat.	the presence of 10 microm-cu2+ increased the lethal effect of hydrogen peroxide on spores of clostridium bifermentans but not on those of clostridium sporogenes pa 3679, clostridium perfringens, bacillus cereus or bacillus subtilis var. niger. cu2+ at 100 mum also increased the lethal effect of heat on spores of c. bifermentans but not on those of b. sutilis var. niger. the rate and extent of cu2+ uptake by spores of c. bifermentans and b. subtilis var. niger were similar, but examination of uns ...	1979	113488
how specific is the effect of penicillins on the conformation of penicillinase? an experimental model.		1979	113659
[cryoresistance during sporulation in bacillus (author's transl)].	the maximal resistance at -- 20 degrees c is obtained as soon as stage v of sporulation in bacillus cereus and for the spore of the mutant marburg strain of b. subtilis bloked at stage v of sporulation.	1979	114081
microbiological transformations of nabilone, a synthetic cannabinoid.	a screening program was conducted to find microorganisms that modify the synthetic cannabinoid nabilone. after purification, the products from three cultures were analyzed by spectral methods to determine their chemical structures. an optically active 9s-hydroxy-6ar,10ar-trans cannabinoid was isolated from a culture of an unidentified soil bacterium designated a24007. from bacillus cereus cultures were isolated a 9s,6'-dihydroxy-6ar,10ar-trans cannabinoid, a 9s-hydroxy-6'-keto-6ar,10ar-trans can ...	1979	114111
two gas-gangrene-like infections due to bacillus cereus.	two cases of postoperative gas-gangrene-like infection due to bacillus cereus are reported, drawing attention to the fact that bacillus cereus, a common environmental bacterium, can occasionally give rise to severe post-operative infection. characteristics of the organism related to the epidemiology and pathogenesis of such infections are discussed.	1979	114263
bacillus cereus: not a contaminant.		1979	114678
polioencephalomalacia in range cattle.	polioencephalomalacia developed in 27 of 225 cattle grazing on 486 hectares of dry, short, grama grass pasture. chemicals in drinking water, toxin from nitrate-utilizing ruminal bacteria, and documented poisonous plants were considered as etiologic agents. attempts to reproduce the disease by injecting mice and dosing sheep with broth filtrate from nitrate-utilizing ruminal bacteria were not successful. mushrooms collected from the pasture and fed to a cow did not reproduce the disease.	1979	115820
anomeric configuration of n-acetylglucosaminyl phosphorylundecaprenols formed in bacillus cereus membranes.	the structural difference was studied between two n-acetylglucosaminyl phosphorylundecaprenols formed by incubation of bacillus cereus membranes with udp-n-acetylglucosamine. on the treatment with 50% phenol, the major one of these glycolipids (lipid 1) yielded a saccharide phosphate, while the other (lipid 2) yielded n-acetylglucosamine along with a saccharide phosphate. the saccharide phosphates from lipids 1 and 2 were identified as alpha-n-acetylglucosamine 1-phosphate and its beta-anomer, r ...	1979	115872
cephalosporin-sensitive penicillin-binding proteins of staphylococcus aureus and bacillus subtilis active in the conversion of [14c]penicillin g to [14c]phenylacetylglycine.	breakdown of the covalent complex formed between [14c]penicillin g and higher molecular weight, cephalosporin-sensitive penicillin-binding proteins was studied using mixtures of the purified proteins isolated from membranes of staphylococcus aureus and bacillus subtilis. these penicillin-binding proteins were found to release the bound 14c label in a first order process characterized by half-lives of 10 to 300 min at 37 degrees c. denaturation of the penicilloyl.penicillin-binding proctein compl ...	1979	115876
the bacillus cereus toxin: isolation of permeability factor.	the bacillus cereus protein has been obtained from culture fluid in homogenic form as indicated by sds-disc electrophoresis and immunodiffusion not described before. the protein has a molecular weight of 100000 daltons. purification was accomplished by the following steps: (1) removal of ballast nitrous components with de-32 cellulose at ph 7.2; (2) removal of the proteins from the culture filtrate (deluted four times by water) with de-32 cellulose at ph 8.6; (3) elution by 0.005 m tris-hcl buff ...	1979	116438
the action of bacillus cereus phospholipase c on central-nervous-system myelin in freshly isolated and freeze-dried resuspended forms [proceedings].		1979	116892
recovery from bacillus cereus sepsis.	a patient with acute leukemia developed two separate episodes of bacillus cereus septicemia during one hospitalization. leukopenia as a consequence of cytotoxic chemotherapy preceded both illnesses. the course of the infections was favorably influenced by the return of adequate numbers of circulating granulocytes and aminoglycoside therapy. only one other compromised host is known to have recovered from this otherwise fatal disease.	1979	117555
effect of dyes on the quantitative recovery of yersinia enterocolitica.	a total of 69 dyes were incorporated separately at different concentrations into an agar medium for evaluation of their effects on the quantitative recovery of five serotypes of yersinia enterocolitica. one strain of pseudomonas aeruginosa and one strain of bacillus cereus were included for comparative purposes. certain dyes were evaluated further for their selective properties with five additional serotypes of y. enterocolitica, three strains of p. aeruginosa, and two of engerobacter spp. metan ...	1979	117750
spore survival during batch dry rendering of abattoir waste.	normal batch dry rendering practice does not ensure sterile products, because bacterial spores are protected against thermal denaturation by the high fat-low water content environment which results from drying the materials at temperatures below those required for sterilization.	1979	117753
dielectric properties of native and decoated spores of bacillus megaterium.	a general model for use in interpreting dielectric data obtained with bacterial endospores is developed and applied to past results for bacillus cereus spores and new results for bacillus megaterium spores. the latter were also subjected to a decoating treatment to yield dormant cells with damaged outer membranes that could be germinated with lysozyme. for both spore types, core ions appeared to be completely immobilized, and decoating of b. megaterium spores did not affect this extreme state of ...	1979	118161
anti-riboflavin activity of 8-o-alkyl derivatives of riboflavin in some gram-positive bacteria.	two new 8-o-alkyl derivatives of riboflavin (rf), i.e., 8-methoxy- (mof), and 8-ethoxy-8-demethyl-d-riboflavin (eof), their tetraacetate, and the tetraacetate of 8-hydroxy-8-demethyl-d-riboflavin (hof) were synthesized. the anti-rf activity of mof, eof and hof was estimated from the ratio cr/ci, where ci is the concentration of test flavin added to the culture medium and cr is the minimum concentration of rf needed to restore the growth inhibition. their activity was also compared with that of r ...	1979	118241
[enterotoxigenecity of bacillus cerous].		1979	118505
studies on bacterial cell wall inhibitors. viii. mode of action of a new antibiotic, azureomycin b, in bacillus cereus t.	azureomycin b, a new antibiotic which contains sugar, amino acid and phenol moieties and inhibits gram-positive bacteria, was found to be a specific inhibitor of peptidoglycan synthesis in bacteria. the antibiotic lysed growing cells of bacillus cereus t at a concentration of 10 micrograms/ml but did not affect resting cells. microscopical observation revealed swelling and lysis of the bacterial rods when treated with azureomycin b. the incorporation of [3h]diaminopimelic acid or [14c]glucosamin ...	1979	118956
effects of lysozyme on bacillus cereus 569: rupture of chains of bacteria and enhancement of sensitivity to autolysins.	bacillus cereus 569 is known to be resistant to lysis by lysozyme because of the presence of deacetylated glucosamine residues in its peptidoglycan, and cultures continued to grow even in the presence of lysozyme at 200 microgram ml-1. however, lysozyme caused rupture of the chains of bacteria and promoted the rate of autolysis in a non-growing cell suspension, causing a doubling of the rate of release of radioactively labelled wall material. heat-inactivated cells did not autolyse and were not ...	1979	119028
[biosynthesis of leucine in a reductoisomerase non inducible mutant of bacillus cereus t].		1979	119554
bacillus cereus septic arthritis following arthrography.	organisms of bacillus species usually are common laboratory contaminants and nonpathogenic in humans. recently, however, it has been suggested that cultures growing common bacillus species may indeed represent significant infections and should not always be disregarded. a 24-year-old man developed bacillus cereus septic arthritis following routine arthrography, which is an example of a serious orthopedic infection that can be caused by this supposedly nonpathogenic organism.	1979	119603
[effect of the amount of the nutrient medium and the action of small concentrations of noxious substances on the heat production in bacterial broth cultures].		1979	119622
bacillus cereus wound infections.		1979	119794
the growth and form of bacterial colonies.	a simple method is described for measuring the profile of bacterial colonies. profiles were determined for colonies of bacillus cereus, escherichia coli and staphylococcus albus of different ages. in spite of differences in cell morphology, the colony profiles had a common basic structure consisting of steeply rising leading edge connected by a ridge to an interior region where height also rose, though less steeply, to a flat or domed centre. the colony mass increased exponentially through part ...	1979	120410
[studies to investigate the ecological importance of the mass development of hydrodictyon reticulatum in infiltration basins for drinking water. iii. identification of the active components by the use of spectroscopic methods and gas chromatography (author's transl)].	antibacterial substances from h. reticulatum, that was harvested from its natural habitat or grown under culture conditions, could be isolated by extraction with ethanol or by steam destillation from cells as well as from culture filtrate. the active substances were tested by gas chromatography, infrared spectroscopy and mass spectrometry and characterized as a fraction of saturated fatty acids. its composition seems to depend on external circumstances or culture conditions, respectively, becaus ...	1979	120652
[inhibition of bacillus cereus beta-lactamase by dicloxacillin].		1979	121024
the spectrum of bacillus bacteremias in heroin addicts.	bacillus bacteremias occurred in two heroin addicts. the first patient had one day of fever and chills after intravenous heroin use. persistent cereus bacteremia consistent with endocarditis was documented and responded to four weeks of antibiotic therapy. the second patient had non-cereus bacillus species isolated from blood cultures three times over eight days, each time after renewed heroin use. the patient remained well, and the bacteremias cleared spontaneously. because bacillus species fre ...	1979	106784
[microbiological aspects of stored hard-boiled eggs].	hard-bioled eggs in the shell were without any pretreatment stored in air at 20 degrees c and 4 degrees c. furthermore the keeping quality of varnish-coated eggs stored in air at 20 degrees c and of untreated eggs stored in 100% carbon dioxide at the same temperature was studied. hard-boiled eggs stored at 4 degrees c and varnish-coated eggs stored at 20 degrees c were of excellent bacteriological quality up to 5 weeks. untreated eggs stored in air at 20 degrees c showed high microbiological con ...	1979	121493
preparation and properties of an immobilized derivative of penicillinase.	penicillinase (beta-lactamase i, ec 3.5.2.6) secreted by bacillus cereus, strain 569/h, was covalently attached to aminoethyl cellulose via glutaraldehyde. the immobilized derivative shows increased thermostability and decreased susceptibility to conformational changes induced by certain substrates of penicillinase. the decline in the rate of hydrolysis of such substrates was consequently suppressed by immobilization. a marked increase in km was observed with all substrates except for the unsubs ...	1979	219920
[electron microscopic study of normal bacillus anthracoides spores and after exposure to a chloroactive disinfectant].	the fine structure of bacillus anthracoides spores is similar in general to that of other, taxonomically related species of spore forming bacteria. however, the former is characterized by a well-developed multilayered exosporium and the heterogeneous structure of an envelope. the lethal effect of a chloroactive disinfectant (2/3 of calcium hypochlorite basic salt) is caused by changes in the structural organization of spores, which interferes with the normal permeability barrier and metabolic pr ...	1979	224288
the role of manganese in growth and sporulation of bacillus subtilis.	phosphoglycerate phosphomutase of bacillus subtilis, bacillus cereus and bacillus megaterium required mn2+ as cofactor, whereas the wheat germ and rabbit liver enzymes did not. in the absence of mn2+, b. subtilis did not sporulate in normal sporulation media but it did sporulate if the proper ratio of glucose or glycerol and malate was used. decoyinine, an inhibitor of guanosine monophosphate synthesis, induced sporulation in the presence of excess glucose and malate to the same extent with and ...	1979	225409
metal-coordinating substrate analogs as inhibitors of metalloenzymes.	a group of active-site metal coordinating inhibitors of zinc proteases (carboxypeptidase a, thermolysin, bacillus cereus neutral protease, and angiotensin-converting enzyme) have been synthesized and their properties investigated. their general structures are r-sh and r-nh-po2(o phi)h, where-s- or -o- serve as metal ligands and r refers to an amino acid or peptide group designed to interact with substrate recognition sites. these inhibitors can be extremely potent; thus, n-(2-mercaptoacetyl)-d-p ...	1979	230502
synthesis of choline and ethanolamine phospholipids with thiophosphoester bonds as substrates for phospholipase c.	spectrophotometric assays of esterases are sensitive, rapid, and quite specific when thioester substrates are used. glycerophospholipids with thiophosphoester bonds may be useful as substrates for phospholipase c (ec 3.1.4.3). these have been made from mercaptoglycerol and mercaptoethanol. the thiols were oxidized to disulfides, acylated, and reduced with dithiothreitol. phosphocholine derivatives were made by the classical methods for oxyphosphoesters. the phosphatidyl choline analogue was conv ...	1979	231487
predicted secondary structures of four penicillin beta-lactamases and a comparison with two lysozymes.	we have predicted the secondary structures of four beta-lactamases (bacillus cereus, bacillus licheniformis, staphylococcus aureus, and escherichia coli r-tem) by the statistical method of chou & fasman as well as by the information theory method of garnier et al. the secondary structures of all four beta-lactamases are of the alpha/beta type (levitt & chothia's nomenclature), with helices at n- and c-termini. there are about eight short regions each of alpha-helical (30--50%) and beta-strand (1 ...	1979	313376