Publications

cumulative correlations of lysozyme, lactoferrin, peroxidase, s-iga, amylase, and total protein concentrations with adherence of oral viridans streptococci to microplates coated with human saliva.	redundancy refers to the observation that many salivary proteins exhibit similar properties in vitro. it is possible that bacterial adherence to salivary pellicle occurs as a cumulative effect of multiple proteins. this study determined the joint and individual contributions of salivary amylase, s-iga, lysozyme, salivary peroxidase, lactoferrin, and total protein concentrations to adherence by oral viridans streptococci in microplates coated with whole saliva from 123 persons. strains used were: ...	1999	10096451
[analysis of serum antibodies to alpha-streptococci in patients with tonsil-related pustulosis palmaris et plantaris].	to determine the systemic immune response to alpha-streptococci (str. sanguis, str. salivarius and str. mitis) and beta streptococcus (str. pyogenes t12) in patients with tonsil related pustulosis palmaris et plantaris (ppp), we measured serum antibody levels to whole cell body antigens of streptococcus (str.) sanguis, str. salivarius, str. mitis or str. pyogenes by enzyme-linked immunosorbent assay (elisa). the serum igg antibody levels to alpha-streptococci, str. sanguis and str. salivarius we ...	1999	10191624
lipoteichoic acid acts as an antagonist and an agonist of lipopolysaccharide on human gingival fibroblasts and monocytes in a cd14-dependent manner.	cd14 has been implicated as a receptor of lipoteichoic acid (lta) and other bacterial components as well as lipopolysaccharide (lps). since the structures of ltas from various gram-positive bacteria are heterogeneous, we analyzed the effects of ltas on the secretion of interleukin-8 (il-8) by high- and low-cd14-expressing (cd14(high) and cd14(low)) human gingival fibroblasts (hgf). while bacillus subtilis lta had an il-8-inducing effect on cd14(high) hgf which was considerably weaker than that o ...	1999	10084995
efficacy of trovafloxacin in treatment of experimental staphylococcal or streptococcal endocarditis.	the efficacy of trovafloxacin against staphylococcus aureus and viridans group streptococci was investigated in vitro and in an experimental model of endocarditis. the mics at which trovafloxacin and ciprofloxacin inhibited 90% of clinical isolates of such bacteria (mic90s) were (i) 0.03 and 2 mg/liter, respectively, for 30 ciprofloxacin-susceptible s. aureus isolates, (ii) 32 and 128 mg/liter, respectively, for 20 ciprofloxacin-resistant s. aureus isolates, and (iii) 0.25 and 8 mg/liter, respec ...	1999	9869569
co-aggregation as a virulent factor of streptococcus sanguis isolated from infective endocarditis.	the pathogenicity of strains of the streptococcus sanguis group, isolated from infective endcarditis, was studied by measuring the development of subcutaneous abscesses in mice after infection with s. sanguis and actinomyces viscosus either singly or as co-aggregated pairs. the pathogenicity of the co-aggregates was also examined in various viable combinations of the two bacterial species. more abscesses were formed by a. viscosus than the s. sanguis group including clinical isolates. abscess fo ...	1999	10692836
in vitro antimicrobial activity of propolis and arnica montana against oral pathogens.	arnica and propolis have been used for thousands of years in folk medicine for several purposes. they possess several biological activities such as anti-inflammatory, antifungal, antiviral and tissue regenerative, among others. although the antibacterial activity of propolis has already been demonstrated, very few studies have been done on bacteria of clinical relevance in dentistry. also, the antimicrobial activity of arnica has not been extensively investigated. therefore the aim here was to e ...	2000	10716618
[lumbosacral spondylodiscitis as the first manifestation of subacute bacterial endocarditis caused by streptococcus sanguis type ii].		2000	10721582
salivary film expresses a complex, macromolecular binding site for streptococcus sanguis.	teeth in the oral cavity are coated with a salivary film or pellicle, which lacks apparent intermolecular organization. this heterogeneous film facilitates binding of early commensal colonizing bacteria, including streptococcus sanguis. to test the hypothesis that sufficient intermolecular organization exists in salivary films to form binding sites for s. sanguis, an in vitro model of saliva-coated teeth was probed with murine anti-idiotypical monoclonal antibodies (mab2, anti-ids). the anti-ids ...	2000	10722745
namibian chewing stick, diospyros lycioides, contains antibacterial compounds against oral pathogens.	the twigs of diospyros lycioides, a plant commonly known as "muthala", are frequently used as chewing sticks for the cleaning of teeth by rural and urban people in namibia. preliminary studies showed that a methanol extract of d. lycioides inhibited growth of selected oral pathogens. subsequent bioassay-guided fractionation led to the isolation of four novel bioactive naphthalene glycosides, diospyrosides a, b, c, and d (1-4), and two known bioactive naphthoquinones, juglone (5) and 7-methyljugl ...	2000	10725172
investigation of bacteremia following orthodontic debanding.	the aim of this study was to investigate the prevalence of bacteremia after orthodontic debanding and debonding. the study group comprised 30 patients (10 men, 20 women). all were treated using the edgewise technique. patients with acceptable oral hygiene and fixed appliances in both jaws were included in the study group. blood samples were obtained using a strict aseptic technique before and after removal of bands and brackets. a 6.6% bacteremia prevalence was observed in both preoperative and ...	2000	10730670
purification, characterization, and molecular analysis of the gene encoding glucosyltransferase from streptococcus oralis.	streptococcus oralis is a member of the oral streptococcal family and an early-colonizing microorganism in the oral cavity of humans. s. oralis is known to produce glucosyltransferase (gtase), which synthesizes glucans from sucrose. the enzyme was purified chromatographically from a culture supernatant of s. oralis atcc 10557. the purified enzyme, gtase-r, had a molecular mass of 173 kda and a pi of 6.3. this enzyme mainly synthesized water-soluble glucans with no primer dependency. the addition ...	2000	10768934
profile of subgingival microbiota in children with primary dentition.	eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). the samples were cultured for bacterial isolat ...	2000	10791707
comparison of gyra and parc mutations and resistance levels among fluoroquinolone-resistant isolates and laboratory-derived mutants of oral streptococci.	laboratory-derived fluoroquinolone-resistant mutants were obtained by serial passage of streptococcus sanguis and streptococcus anginosus isolates on agar containing increasing concentrations of old and new fluoroquinolones, ofloxacin and du-6859a, respectively. sequencing of an s. sanguis isolate exposed to du-6859a showed that resistance was associated with two mutations in the quinolone resistance determining region (qrdr) of the gyra gene (ser83-->phe; glu87-->lys), and with a mutation in th ...	2000	10837428
effect of an essential oil-containing antiseptic mouthrinse on induction of platelet aggregation by oral bacteria in vitro.	with an increasing body of data suggesting an association between periodontitis and cardiovascular disease, studies have been conducted to elucidate potential mechanisms by which oral bacteria might exert systemic effects. 2 oral bacteria, streptococcus sanguis and porphyromonas gingivalis, have been shown to induce platelet aggregation in vitro. this study was conducted to determine the effect of treatment with an essential oil mouthrinse (listerine antiseptic) on the platelet-aggregating activ ...	2000	10847543
differential patterns of apoptosis in resolving and nonresolving bacterial pneumonia.	infection with either streptococcus sanguis or streptococcus pneumoniae type 25 causes acute pneumonitis in rats. pneumonia caused by s. sanguis resolves over the course of 8 d, whereas pneumonia caused by s. pneumoniae type 25 progresses to fibrosis. to examine the role of apoptosis in these models, we performed assays with the terminal deoxynucleotidyltransferase-uridine nucleotide end-labeling technique on tissue sections from rat lungs at various times, and quantified the results with image ...	2000	10852786
natural history of streptococcus sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity.	the heterogeneous group of oral bacteria within the sanguinis (sanguis) streptococci comprise members of the indigenous biota of the human oral cavity. while the association of streptococcus sanguinis with bacterial endocarditis is well described in the literature, s. sanguinis is thought to play a benign, if not a beneficial, role in the oral cavity. little is known, however, about the natural history of s. sanguinis and its specific relationship with other oral bacteria. as part of a longitudi ...	2000	10858217
ib-367, a protegrin peptide with in vitro and in vivo activities against the microflora associated with oral mucositis.	although the microflora associated with oral mucositis initiated by cytotoxic therapy is not well characterized, several studies suggest that reduction of the microbial load in the oral cavity has some clinical benefit. the mics of ib-367, a synthetic protegrin analog, ranged from 0.13 to 64 microgram/ml for gram-positive bacteria (streptococcus mitis, streptococcus sanguis, streptococcus salivarius, and staphylococcus aureus) and from 0.06 to 8 microgram/ml for gram-negative species (klebsiella ...	2000	10858334
studies concerning the glucosyltransferase of streptococcus sanguis.	we have shown in previous studies that the glucosyltransferase (gtf) enzymes of streptococcus mutans have distinct properties when adsorbed to a surface. in the present study, we compared the activity of gtf from streptococcus sanguis, designated gtfss, in solution and on the surface of saliva-coated hydroxyapatite (sha) beads, and determined the ability of its product glucan to support the adherence of oral microorganisms. gtf from s. sanguis 804 nctc 10904 was purified from culture supernatant ...	2000	10867431
[meningitis after spinal anesthesia].	the occurrence of meningitis after spinal anaesthesia is a very rare event. we report a case of streptococcus sanguis meningitis following spinal anaesthesia for orthopaedic material removal. the presence of gram positive cocci (streptococcus sanguis) in the cerebrospinal fluid was in favour of an exogenous contamination, originating either from the patient's skin or the anaesthesiologist's oropharynx. the outcome was uneventful. the responsibility of the latter can result in legal consequences. ...	2000	10874434
role of monocytes in experimental staphylococcus aureus endocarditis.	in the pathogenesis of bacterial endocarditis (be), the clotting system plays a cardinal role in the formation and maintenance of the endocardial vegetations. the extrinsic pathway is involved in the activation of the coagulation pathway with tissue factor (tf) as the key protein. staphylococcus aureus is a frequently isolated bacterium from patients with be. we therefore investigated whether s. aureus can induce tf activity (tfa) on fibrin-adherent monocytes, used as an in vitro model of be. we ...	2000	10899897
colorimetric indicators of microbial contamination in corneal preservation medium.	to compare acid-base and oxidation-reduction indicators and to investigate the effect of buffer and temperature on the colorimetric detection of microbial growth in corneal preservation media.	2000	10928770
effect of the interleukin-1 genotype on monocyte il-1beta expression in subjects with adult periodontitis.	an association has been reported between polymorphisms in the genes encoding il-1alpha (-889) and il-1beta (+3953) (periodontitis susceptibility trait, pst), and an increased severity of periodontitis (18). the il-1beta polymorphism was reported to correlate with increased il-1beta expression by monocytes in response to bacterial stimulants. in the present study, we determined if pst positive subjects with periodontitis exhibit elevated production of il-1beta, compared to pst negative periodonti ...	2000	10929872
taxonomic study of "tufted mitior" strains of streptococci (streptococcus sanguinis biotype 11); recognition of a new genospecies.	the taxonomic position of tufted strains of streptococci, phenotypically resembling streptococcus mitis and previously referred to as 'tufted mitior' was investigated. by 16s rrna sequence analysis, it was clear that the "tufted mitior" strains belonged to the mitis group of species within the genus streptococcus. it was confirmed that these strains were taxonomically independent at the species level, sharing less than 43%, dna-dna similarity with all established species of the mitis group. howe ...	2000	10930077
trovafloxacin treatment of viridans group streptococcus experimental endocarditis.	the activity of trovafloxacin was compared with those of vancomycin and penicillin in a model of streptococcus sanguis species group (trovafloxacin mic, 0.125 microg/ml) and streptococcus mitis species group (trovafloxacin mic, 0.125 microg/ml) experimental endocarditis. rabbits with catheter-induced aortic valve vegetations were given no treatment, trovafloxacin at 15 mg/kg of body weight three times a day (t.i.d.), vancomycin at 15 mg/kg twice a day, or penicillin at 1. 2 x 10(6) iu t.i.d. aft ...	2000	10952616
antibody-targeted lethal photosensitization of porphyromonas gingivalis.	we have previously demonstrated that porphyromonas gingivalis is susceptible to killing by toluidine blue o (tbo) when irradiated with light from a helium-neon (hene) laser. the aim of this study was to determine whether a tbo-antibody conjugate (ab-tbo) could be used to specifically target p. gingivalis to lethal photosensitization in the presence of streptococcus sanguis or human gingival fibroblasts (hgfs). when a mixture of p. gingivalis and s. sanguis was exposed to 4 microg of tbo/ml and i ...	2000	10991833
presence of bacterial 16s ribosomal rna gene segments in human intestinal lymph follicles.	there is currently no information regarding microbial agents inside the intestinal lymph follicles.	2000	10994621
effects of antibodies to glucosyltransferase on soluble and insolubilized enzymes.	previous studies have shown that glucosyltransferase enzymes (gtfs) of streptococcus mutans adsorbed to saliva coated hydroxyapatite (sha) have distinct properties from the same enzymes in solution. the purpose of the present study was to determine the effects on enzyme activity of polyclonal antibodies raised to gtfs in a soluble form and bound to sha.	2000	11002411
effects of apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatite.	propolis, a resinous hive product collected by apis mellifera bees, has been used for thousands of years in folk medicine. ethanolic extracts of propolis (eep) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (gtf) enzymes in solution. these enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. in the present study, the effects of propolis from two different regions of brazil on the activity of separate, purif ...	2000	11014909
microbial composition of supra- and subgingival plaque in subjects with adult periodontitis.	the purpose of the present study was to compare and relate the microbial composition of supra and subgingival plaque in 23 adult periodontitis subjects (mean age 51 +/- 14 years).	2000	11034118
automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque.	the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ...	2000	11037755
competition for glucose between candida albicans and oral bacteria grown in mixed culture in a chemostat.	the competition for glucose as a growth-limiting substrate between candida albicans and a mixed community of oral bacteria was investigated. a chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. a mixed population of oral bacteria was established and after a steady state had been reached the chemostat was inoculated with c. albicans. seven bacterial species streptococcus sanguis, s. sobrinus, s. mitis, lactobacillus casei, veillonella dispar, ...	2000	11073150
fever and pain over the right hypochondrium. liver abscess as the presenting form of stromal intestinal tumor.		2000	11076187
sensitization of actinomyces naeslundii and streptococcus sanguis in biofilms and suspensions to acid damage by fluoride and other weak acids.	fluoride and other weak acids, such as benzoate, indomethacin, salicylate and sorbate, were found to be sensitizers for acid killing of cells of actinomyces naeslundii atcc 19246 and streptococcus sanguis nctc 10904 in suspensions or in mono-organism biofilms on glass slides. these bacteria are among the more acid-sensitive organisms from dental plaque and were killed when acidified to ph values between 3.5 and 4.0. biofilm cells were more resistant than cells in suspensions, especially in terms ...	2000	11081793
fractional inhibitory concentration index of combinations of antibacterial agents against cariogenic organisms.	to examine the inhibitory effects between paired combinations of four antibacterial agents that have been investigated in dentistry against six bacteria associated with dentinal caries.	2000	11082524
images in cardiology: silent patent ductus arteriosus endarteritis.		2000	11083739
[general syndrome and fever].		2000	11083959
similarity of bacteriocin activity profiles of mutans streptococci within the family when the children acquire the strains after the age of 5.	it has been shown that there is a window of infectivity for mutans streptococci between the ages of 19 and 31 months, when many children acquire mutans streptococci transmitted from their mothers. part of the children that escape this window acquire mutans streptococci at a later age. in this group, maternal transmission is expected to be less prevalent. the present study compared the bacteriocin activity profiles of mutans streptococci isolated from mothers, fathers and children when the childr ...	2000	11093022
strain-related acid production by oral streptococci.	acid production, in particular at low ph, is thought to be an important ecological determinant in dental caries. the aim of the present study was to determine the acid producing capability at different ph levels of 47 streptococcal strains, representing 9 species, isolated from human dental plaque. the bacteria were grown until mid log-phase under anaerobic conditions and acid production was measured in a ph-stat system at ph 7.0, 6.0, 5.5 and 5.0. at all ph values, the mean velocity of acid pro ...	2000	11093023
aneurysm of the anterior leaflet of the mitral valve secondary to aortic valve endocarditis.	aneurysm of the anterior mitral leaflet is a rare complication of infective aortic valve endocarditis, the natural evolution of which is generally its rupture, with subsequent acute and severe mitral regurgitation. its presence cannot be recognized with transthoracic echocardiography and even in surgery. we describe a 78-year-old man with aortic valve endocarditis, in whom transesophageal echocardiography was essential for the diagnosis of this complication, its therapeutic management, and the p ...	2000	11093109
dental plaque formation.	dental plaque is a complex biofilm that accumulates on the hard tissues (teeth) in the oral cavity. although over 500 bacterial species comprise plaque, colonization follows a regimented pattern with adhesion of initial colonizers to the enamel salivary pellicle followed by secondary colonization through interbacterial adhesion. a variety of adhesins and molecular interactions underlie these adhesive interactions and contribute to plaque development and ultimately to diseases such as caries and ...	2000	11113379
effects of organic acid anions on growth, glycolysis, and intracellular ph of oral streptococci.	oral streptococci produce large quantities of organic acids as the end-products of carbohydrate fermentation. in an approach to determine if oral streptococci exhibit differential sensitivities to organic acid anions, we determined the effects of formate, lactate, and acetate on intracellular ph maintenance, glycolysis, and growth of streptococcus mutans and streptococcus sanguis. growth was determined as maximum culture optical density in the presence of the organic acid anions at ph 7.1, 6.7, ...	2000	10690666
post-ph effect in oral streptococci.	to study the post-ph effect (pphe) in 11 strains of oral streptococci belonging to streptococcus sanguis, s. mitis, s. gordonii, s. mutans and s. sobrinus by using the bact/alert microbial detection system.	2000	11168090
adhesion of candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface.	adhesion of candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. c. albicans cells also adhere to several species of oral streptococci including streptococcus gordonii, streptococcus oralis and streptococcus sanguinis in what are believed to be multi-modal interactions. it is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of c. albicans cells by up to 2-3-fol ...	2000	10658650
synthesis, microbicidal activity, and solution structure of the dodecapeptide from bovine neutrophils.	the dodecapepetide sequence r-l-c-r-i-v-v-i-r-v-c-r with a disulfide bridge between the cysteine residues found in bovine neutrophils was synthesized by solid-phase procedures. its antimicrobial activity against oral microorganisms such as actinobacillus actinomycetemcomitans, porphyromonas gingivalis, streptococcus mutans, and streptococcus gordonii was examined, and its structural features were examined by cd and determined by two-dimensional (2d) nmr. the strains p. gingivalis (w50 and 381), ...	2000	10685049
in vitro study of the penetration of streptococcus sanguis and prevotella intermedia strains into human dentinal tubules.	the persistent presence of bacteria in the root canal system often leads to the failure of treatment. the purpose of this study was to determine in vitro penetration of streptococcus sanguis and prevotella intermedia into dentinal tubules. samples obtained from human teeth were inoculated with a strain of s. sanguis (nctc 7853) and p. intermedia (nctc 93336) for 20 days. bacterial penetration into tubules was investigated at scanning electron microscopy and light microscopic level. the results s ...	2000	11199726
comparative evaluation of antibacterial effects of nd:yag laser irradiation in root canals and dentinal tubules.	the antibacterial effects of the nd:yag laser on contaminated root canals and dentinal tubules were observed as the aim of this study. the samples were inoculated with streptococcus sanguis (nctc 7853) and prevotella intermedia (nctc 93336), and the effects of nd:yag laser were tested on these teeth. the specimens were lased with 1.8 w and 2.4 w nd:yag laser for 30 s, and the presence of bacteria in tubules was observed under light microscopy. the 1.8 w laser sterilized the tubules in 86.3% of s ...	2000	11199732
addition of antibacterial agents to mma-tbb dentin bonding systems--influence on tensile bond strength and antibacterial effect.	to produce a bonding system which has both high bond strength and antibacterial properties, an antibacterial agent (vancomycin: vcm or metronidazol: mn) was added to the pmma powder of 4-meta/mma-tbb resin (cb). the influence of the addition of an antibacterial agent on tensile bond strength to dentin and the antibacterial effect were investigated in this study. forty-seven freshly extracted bovine first or second incisors were used to measure the tensile bond strength to dentin. the bond streng ...	2000	11219091
investigation on interaction between streptococcus sanguis and porphyromonas gingivalis in specific pathogen-free rats.	to examine whether endogenous streptococcus can prevent or reduce the colonization of the virulent porphyromonas gingivalis strain.	2000	11314089
effects of three different infant dentifrices on biofilms and oral microorganisms.	the purpose of this work was to evaluate the effects of infant dentifrices: a--with lactoperoxidase, glucose oxidase and lactoferrin; b--with 1100 ppm of naf and sodium lauryl sulfate; c--with extract of calendula. the dentifrices were test on biofilms formed in vitro from saliva and dental plaque of infants, using reference strains a. viscosus (atcc 43146); c. albicans (atcc 51501); l. casei (atcc 4646); s. mitis (atcc 49456); s. mutans (atcc 25175); s. oralis (atcc 35037); s. sanguis (atcc 105 ...	2000	11314149
comparative in vitro activity of moxifloxacin against gram-positive clinical isolates.	the in vitro activity of moxifloxacin was compared with that of 15 antibacterial agents against 513 gram-positive microorganisms. the mic(90) (mg/l) of moxifloxacin was 0.06 for quinolone-susceptible staphylococcus aureus and staphylococcus epidermidis, 0.12 for streptococcus pyogenes and streptococcus agalactiae; 0.25 for streptococcus pneumoniae, streptococcus mitis, streptococcus bovis, streptococcus anginosus and actinomyces pyogenes; 0.5 for streptococcus sanguis and listeria monocytogenes, ...	2000	10629010
diversity and contribution of the intestinal bacterial community to the development of musca domestica (diptera: muscidae) larvae.	the bacterial diversity in the intestinal tract of musca domestica l. was examined in larvae collected from turkey bedding and corn silage. aerobic culturing yielded 25 bacterial species, including 11 from larvae collected from turkey bedding and 14 from larvae collected from corn silage. providencia rettgeri (hadley, elkins & caldwell) was the only species common to both environments. two mammalian pathogens, yersinia pseudotuberculosis (pfeiffer) and ochrobactrum anthropi (holmes), were isolat ...	2000	11126551
postantibiotic effects and postantibiotic sub-mic effects of amoxicillin on streptococcus gordonii and streptococcus sanguis.	amoxicillin is one of the most frequently recommended antibiotics for prophylaxis of infective endocarditis in dental/oral procedures. in this study, the postantibiotic effect (pae), postantibiotic sub-mic (pasme) and sub-mic effect (sme) of amoxicillin on oral streptococci, streptococcus gordonii and streptococcus sanguis, which are two of the major etiological agents in infective endocarditis, were investigated. the pae was induced by 10 x mic of amoxicillin for 2 h and the antibiotic was elim ...	2000	11128556
the galu gene of streptococcus pneumoniae that codes for a udp-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies.	the enzyme utp-glucose-1-phosphate uridylyltransferase (udp-glucose pyrophosphorylase, udpg:pp) is synthesized by practically all organisms, although prokaryotic udpg:pps are evolutionarily unrelated to the eukaryotic counterparts. the primary structure of prokaryotic udpg:pps is well conserved, although little information exists on the polymorphism of the genes coding for these enzymes. it has been reported that the galu gene encoding the streptococcus pneumoniae udpg:pp is absolutely required ...	2000	11137293
[bact-alert system for hemocultures: evaluation of terminal subcultures].	few laboratory microbiological procedures are as important as the isolation of microorganisms from blood. to evaluate the usefulness of the terminal subcultures, 5669 blood cultures giving negative results after 7 days of incubation in the bact/alert system (organon teknika) were studied. bottles were distributed as follows: 1562 adult aerobic bottles, 119 adult anaerobic bottles, 3960 pediatric bottles and 28 fan bottles. from 5669 blood cultures, 10 subcultures that yielded growth had not been ...	2000	11149151
the oral microbiota of children undergoing liver transplantation.	this study investigated the oral microbiota of children undergoing liver transplantation. oral swabs were taken using a standardized procedure from 27 children before liver transplantation and at 3 and 100 days post-transplantation and from 27 healthy controls at baseline and 90 days. viridans streptococci, yeasts, staphylococci, enterococci and enterobacteriaceae were enumerated and identified using conventional techniques. the oral microbiota of the patients changed significantly immediately p ...	2000	11154404
porphyromonas gingivalis platelet aggregation activity: outer membrane vesicles are potent activators of murine platelets.	recent evidence has established an association between chronic periodontitis and cardiovascular disease. periodontitis is a chronic inflammatory disease caused by a small group of gram-negative bacteria, of which porphyromonas gingivalis is considered an important causative agent. it has been proposed that dental plaque bacteria and their products can disseminate into the bloodstream from the site of infection and promote thromboembolic events associated with atherosclerosis and myocardial infar ...	2000	11154438
profile of subgingival microbiota in children with mixed dentition.	a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...	2000	11155173
the clinical and microbiological effects of systemic ornidazole in sites with and without subgingival debridement in early-onset periodontitis patients.	the purpose of this study was to evaluate the clinical and microbiological effects of systemic ornidazole (orn) in sites with or without subgingival debridement in early-onset periodontitis (eop) patients.	2000	11156043
adhesion in vitro of oral streptococci to porcelain, composite resin cement and human enamel.	we investigated the initial adhesion in vitro of oral streptococci to porcelain inlays. four strains of streptococci, streptococcus mitis nctc12261, streptococcus oralis atcc9811, streptococcus sanguis atcc10556 and streptococcus sobrinus omz176, were used in this study. the disc specimens were made of porcelain, composite resin cement and human enamel. these specimens, with or without a saliva-coat, were immersed in a suspension of each streptococci strain at 37 degrees c for 1 hr, and the numb ...	2000	11816366
[a comparative study on the capacities of different strains of streptococcus sanguis for p-aminobenzoic acid production].	this study was intended to compare the capacities of different strains of strep, sanguis for p-aminobenzoic acid (paba) production. the synthesis of paba during the growth of four strains of strep. sanguis was measured by the reversed-phase high-performance liquid chromatographic method. the results showed that the concentrations of paba synthesized by s. sanguis 10556, s. sanguis 10557, s. sanguis s34 and s. sanguis h7-4. were 1.979 +/- 0.081 micrograms/ml, 1.383 +/- 0.193 micrograms/ml, 1.983 ...	2000	12545834
[isolated primary streptococcal tricuspid endocarditis. a case report].	the authors report a new case of primitive isolated endocarditis of the tricuspid valve. the microorganism responsible, streptococcus sanguis, is unusual in its localization and in this environment. the uncomplicated evolution poses the problem of deciding what action should be taken vis-à-vis the persistence of an important isolated vegetation after effective antibiotic treatment. therapeutic abstention was chosen rather than surgical intervention in light of the literature. a spontaneous pulmo ...	2000	12555493
periodontitis as a risk factor for cardiovascular disease.		2000	12167894
[the disinfection efficiency comparison of different treatments on dental impression and gypsum casts].	in this study, the disinfecting efficiency of five disinfecting methods to three bacterial: staphylococcus epidermidis, streptococcus sanguis and bacillus subtilis were evaluated.	2000	12539655
[expression of pyruvate oxidase gene sopox from streptococcus sanguis in e. coli].	to reconstruct the expression plasmid of sopox gene for further understanding the regulation of its expression.	2001	12733344
[pyruvate oxidase gene from streptococcus sanguis: molecular cloning and sequence analysis of the gene].	to clone and sequence the gene of pyruvate oxidase (sopox) from streptococcus sanguis.	2001	12536558
antimicrobial activity of four root canal sealers against endodontic pathogens.	the antibacterial effects of various types of widely used endodontic sealers have not been compared systematically on facultative or obligate anaerobic endodontic pathogens. the aim of this study was to evaluate the antimicrobial properties of four commonly used endodontic sealers: two epoxy-resin-based sealers (ah26, ah plus), one zinc-oxide eugenol-based sealer (n2), and one calcium hydroxide-based sealer (sealapex). the testing microbes were four facultative anaerobic species (streptococcus m ...	2001	11800436
the antimicrobial properties of a urea-based handwash lotion with triclosan.	the purpose of this study was to determine and compare the antimicrobial action of a urea-based handwash lotion with triclosan with a lipid-depleting detergent-based handwash product with 4% chlorhexidine gluconate. the zones of inhibition produced by the two handwash lotions and the one produced by the positive control were similar against all strains of bacteria tested, indicating that a urea-based handwash lotion with triclosan is as effective as a lipid-depleting handwash lotion in inhibitin ...	2001	12024756
[clubbed fingers].		2001	11794900
the predominant microflora of nursing caries lesions.	the predominant microflora recovered from infected dentine of 52 carious teeth from 14 children with nursing caries was determined using both selective and non-selective media for the isolation of specific genera and acidified media (ph 5.2) to isolate the predominant aciduric microorganisms, and compared with the microflora of sound enamel surfaces in caries-free children. streptococcus mutans formed a significantly greater proportion of the lesion flora while streptococcus oralis, streptococcu ...	2001	11799279
antibacterial activity of hydrastis canadensis extract and its major isolated alkaloids.	the antibacterial activity of extract and isolated major alkaloids (berberine, beta-hydrastine, canadine and canadaline) of hydrastis canadensis l. (ranunculaceae) was evaluated against 6 strains of microorganism: staphylococcus aureus (atcc 25 993 and atcc 6538p), streptococcus sanguis (atcc 10 556), escherichia coli (atcc 25 922), pseudomonas aeruginosa (atcc 27 853). bactericidal activity was evaluated by contact test by measuring the "killing time" on a low density bacterial inoculum, and ba ...	2001	11509983
the ability of direct fluorescence-based, two-colour assays to detect different physiological states of oral streptococci.	to investigate the ability of six fluorescent-based, two-colour viability assays to detect different physiological growth stages of two oral streptococci species.	2001	11555201
alteration of the coadherence of candida albicans with oral bacteria by dietary sugars.	interactions between bacterial oral flora and candida albicans are important in denture plaque formation. this study therefore first aimed to quantify the coadherence of c. albicans and bacteria by the use of a bioluminescent adenosine triphosphate (atp) assay based on the firefly luciferase-luciferin system. the second aim was to examine the effect of i) dietary sugars (used for preculture) and ii) enzymatic digestion of fungi on the coadherence. when yeast was preincubated in yeast nitrogen ba ...	2001	11555304
actinomyces naeslundii fimbrial protein orf977 shows similarity to a streptococcal adhesin.	the nucleotide sequence of the chromosomal dna, upstream of actinomyces naeslundii t14v fimbrial gene fima, was determined. one open reading frame (orf977) encoding 977 amino acids was found, preceded by a gene homologous to elongation factor tu. database searches revealed that orf977 was homologous to csha, a streptococcus gordonii protein involved in cell adhesion. previous studies had already determined two genes in the type 2 fimbrial gene cluster of a. naeslundii t14v: the structural subuni ...	2001	11555311
phosphorylcholine-dependent cross-reactivity between dental plaque bacteria and oxidized low-density lipoproteins.	antibodies reactive with phosphorylcholine (pc) are ubiquitous in human sera, but the antigens stimulating their production and their function are not clear. previous studies have shown that a significant proportion of dental plaque bacteria contain pc as determined by reactivity with pc-specific mouse myeloma proteins and monoclonal antibodies. additionally, serum antibody concentrations of immunoglobulin (igg) g anti-pc are higher in sera of individuals who have experienced periodontal attachm ...	2001	11598029
in vitro antimicrobial activities of bakuchiol against oral microorganisms.	bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ...	2001	11600349
susceptibilities of oral bacteria and yeast to mammalian cathelicidins.	the effects of cathelicidins against oral bacteria and clinically important oral yeasts are not known. we tested the susceptibilities of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus sanguis, candida krusei, candida tropicalis and candida albicans to the following cathelicidins: fall39, smap29, and cap18. smap29 and cap18 were antimicrobial, whereas fall39 did not exhibit antimicrobial activity. future studies are needed to determine the p ...	2001	11600383
cariogenic potential of lactosylfructoside as determined by acidogenicity of oral streptococci in vitro and human dental plaque in situ.	the cariogenic potential of lactosylfructoside [o-beta-d-galactopyranosyl-(1-->4)-o-alpha-d-glucopyranosyl-(1<-->2)-beta-d-fructofuranoside] was estimated by experiments on oral streptococci in vitro and human dental plaque in situ. lactosylfructoside was unable to support growth of the strains of streptococcus mutans and s. sobrinus used in this study. however, it was able to support growth of strains of s. sanguis, s. mitis and s. oralis. acid was produced rapidly by cell suspensions of s. ora ...	2001	11641569
plaque removal characteristics of electric toothbrushes using an in vitro plaque model.	the purpose of the study was to investigate and quantify the efficacy of plaque removal by commercially available electric toothbrushes using an in vitro system based on artificial plaque grown on glass slides.	2001	11686826
proteolytic degradation of oral biofilms in vitro and in vivo: potential of proteases originating from euphausia superba for plaque control.	this paper deals with enzymatic removal of dental plaque, in vitro as well as in vivo, using proteases from the antarctic krill shrimp (euphausia superba), referred to as krillase. krillase exhibits both endo- and exopeptidase activity but has no microbicidal effect. in model systems with pure cultures of oral microorganisms. krillase demonstrated inhibition of microbial adhesion to saliva-coated hydroxyapatite. furthermore, a protocol for the growth of reproducible in vitro plaque films has bee ...	2001	11695752
co-adhesion and removal of adhering bacteria from salivary pellicles by three different modes of brushing.	this study compares removal of pairs of co-adhering and non-co-adhering oral actinomyces and streptococci by hand, electric and sonic brushing from salivary pellicles. in addition, re-deposition of a co-adhering and non-co-adhering streptococcal strain to brushed pellicles was studied. first, actinomyces were allowed to adhere to a pellicle in a flow chamber, after which streptococci suspended in saliva were perfused through the chamber at 33 c. pellicles with adhering bacterial pairs were brush ...	2001	11695753
plaque formation on surface modified dental implants. an in vitro study.	bacterial adhesion on titanium implant surfaces has a strong influence on healing and long-term outcome of dental implants. parameters like surface roughness and chemical composition of the implant surface were found to have a significant impact on plaque formation. the purpose of this study was to evaluate the influence of two physical hard coatings on bacterial adhesion in comparison with control surfaces of equivalent roughness. two members of the oral microflora, streptococcus mutans and str ...	2001	11737097
dysregulation of monocyte oxidative burst in streptococcal endocarditis.	streptococcal subacute endocarditis is characterized by low-grade systemic inflammation. although structural cardiac defects are pivotal, phagocytic cells, i.e. monocytes and neutrophils, are involved in the induction and the course of bacterial endocarditis. decreased production of reactive oxygen metabolites was described in long-lasting infections. we hypothesized that the oxidative burst of phagocytes induced by the infecting organism is defective in patients with streptococcal endocarditis.	2001	11737229
association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid.	the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions.	2001	11737506
anti-fibrin antibody binding in valvular vegetations and kidney lesions during experimental endocarditis.	in streptococcus sanguinis (sanguis) induced experimental endocarditis, we sought evidence that the development of aortic valvular vegetation depends on the availability of fibrin. endocarditis was induced in new zealand white rabbits by catheter placement into the left ventricle and inoculation of the bacteria. fibrin was localized in the developing vegetation with 99mtechnetium (tc)-labeled anti-fibrin antibody one or three days later. when rabbit anti-fibrin antibody was given intravenously o ...	2001	11762752
bactericidal effect of delmopinol on attached and planktonic streptococcus sanguinis cells.	the aim of this investigation was to determine the antibacterial effect of varying concentrations of delmopinol-hcl on attached as well as on planktonic streptooccus sanguinis cells in vitro. in addition, a possible antiadhesive effect on attached micro-organisms was to be investigated. s. sanguinis cells were allowed to attach to glass surfaces. these as well as planktonic cells were exposed to delmopinol-hci in concentrations ranging from 0.2% to 0.00005% for 2 min. the percentage of vital bac ...	2001	11767281
[effects of streptococcus sanguis on blood composition, blood pressure and cardiac dysfunction in rabbis].	to investigate effect of streptococcus sanguis on blood cell and cardiopulmonary changes.	2001	11769649
[cloning and analyzing of the conserved protein gene in streptococcus sanguinis atcc10556].	to clone and analyze the conserved protein gene in streptococcus sanguinis(s.s) atcc10556.	2001	11774210
[implication and mode of action of infectious agents in the formation of atheromatous plaques. infection and atherosclerosis].	different kinds of infectious agents seem to be implied in the atherosclerotic process. indeed, some bacteria and viruses have been identified in atherosclerotic lesions: chlamydia pneumoniae, helicobacter pylori, streptococcus sanguis, porphyromonas gingivalis, herpes simplex viruses type 1 and 2, coxsakievirus b and hepatitis virus a. by their ability to induce antigenic and functional changes in the cells of the vascular wall (endothelial cells, smooth muscle cells, monocytes/macrophages, and ...	2001	11776694
commercial preparations of lipoteichoic acid contain endotoxin that contributes to activation of mouse macrophages in vitro.	lipoteichoic acids (lta), cell wall components of gram-positive bacteria, have been reported to induce various inflammatory mediators and to play a key role in gram-positive-microbe-mediated septic shock. in a large number of these studies, investigators used commercially available lta purified from a variety of gram-positive bacteria, including staphylococcus aureus, bacillus subtilis, and streptococcus sanguis. we report here that, although these commercially available lta could be readily sho ...	2001	11159964
selection of oral microbial adhesion antagonists using biotinylated streptococcus sanguis and a human mixed oral microflora.	a microtitre assay has been developed using hydroxyapatite-coated wells and streptococcus sanguis nctc 10904 at 10(7) cells per ml. a number of models representing toothpaste and mouthwash usage were adopted to detect the anti-adherent efficacy of a polyvinylmethylether maleic acid copolymer (pvm/ma), polyoxypropylene/polyoxyethylene block copolymer (po/eo), two casein-derived peptides and selected silicones. the results not only confirmed the anti-adherence property of the selected components b ...	2001	11163320
recommended conservation of the names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, and seven other names included in the approved lists of bacterial names. request for an opinion.	with reference to the first principle of the international code of nomenclature of bacteria, which emphasizes stability of names, it is proposed that the original names streptococcus sanguis, streptococcus rattus, streptococcus cricetus, erwinia ananas, eubacterium tarantellus, lactobacillus sake, nitrosococcus oceanus, pseudomonas betle, rickettsia canada and streptomyces rangoon, all included in the approved lists of bacterial names, be conserved. request for an opinion.	2001	11321121
quinolone-resistance mechanisms and in vitro susceptibility patterns among european isolates of streptococcus mitis, streptococcus sanguis, and streptococcus pneumoniae.		2001	11347679
a case-control study of respiratory disease in thoroughbred racehorses in sydney, australia.	in order to investigate the role of infectious agents in the aetiology of lower respiratory tract disease in thoroughbred racehorses, a matched case-control study was conducted. cases were identified by the presence of coughing, and were compared to a control population matched on time of sample collection and location within the same training establishment. tracheal wash samples were collected from 100 cases and 148 controls. case horses were more likely than controls to have endoscopic and cyt ...	2001	11352347
are dental infections a cause of brain abscess? case report and review of the literature.	dental pathology and/or treatment have been linked to a small number of brain abscesses as possible sources of infection. a further case is presented, in which a dental site is implicated. a review of the evidence was undertaken. a wide range of dental procedures had been implicated. in some cases the brain isolate was not of dental origin. in many, the diagnosis was one of exclusion. in order to confirm the role of odontogenic infection in the pathogenesis of brain abscess, modern sampling tech ...	2001	11354924
amine and tin fluoride inhibition of streptococcus sanguis adhesion under continuous flow.	this study evaluated the ability of topically applied amine fluoride (amf) and amf-tin fluoride to inhibit the adhesion of streptococcus sanguis within a parallel plate flow cell system. one of three amf compounds and two tin fluoride preparations significantly reduced the net bacterial adhesion to conditioned glass over a 1-h period. tin(iv) fluoride inhibited s. sanguis adhesion to the greatest extent, and this was shown to be dependent on the formation of the conditioning film prior to agent ...	2001	11358541
antibacterial activity of particulate bioglass against supra- and subgingival bacteria.	particulate bioglass is a bioactive material used in the repair of periodontal defects. this material undergoes a series of surface reactions in an aqueous environment which lead to osseointegration. the aim of this study was to determine whether these reactions exerted an antibacterial effect on a range of oral bacteria. streptococcus sanguis, streptococcus mutans and actinomyces viscosus were suspended in nutrient broth (nb), artificial saliva (as) or dulbecco's modified eagle medium plus 10% ...	2001	11374470
predominant cultivable subgingival microbiota of healthy and hiv-infected ethnic chinese.	although clinically distinctive types of periodontal disease are known to be associated with hiv infection, the pathogenesis remains unclear. in the present study, the subgingival microflora of 21 hiv-infected and 11 hiv-free ethnic chinese were studied using the direct microscopic and anaerobic culture methods. motile curved rods were found to be three times higher in the hiv-infected group under direct microscopy. otherwise, there were no significant differences between the diseased and health ...	2001	11398993
the effect of oral bacteria on candida albicans germ-tube formation.	a total of eight bacterial isolates belonging to six species, and a select group of 12 oral candida albicans isolates, were used to study the effect of bacteria on germ-tube formation. briefly, each bacterial suspension (10(5-6) cells/ml) was mixed with a c. albicans suspension (10(7) cells/ml) and incubated at 37 degrees c for 90 min with bovine serum, and the percentage germ-tube-positive candida cells was quantified using a haemocytometer, under light microscopy. in general, out of eight bact ...	2001	11398996
investigation of bacteremia after orthodontic banding and debanding following chlorhexidine mouth wash application.	this study investigates the prevalence of bacteremia after orthodontic banding and debanding, following the application of a 0.2% chlorhexidine gluconate mouthwash. the banding and debanding groups were each composed of 40 young adult patients. in the banding group, patients were asked to rinse their mouth with chlorhexidine gluconate for 60 seconds just prior to fitting of the bands. in the debanding group, they were asked to use the mouthwash immediately before removal of bands and brackets. i ...	2001	11407771
inhibitory effects of moabs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of streptococcus mutans.	a surface protein antigen (pac) of streptococcus mutans, particularly the a-region of the molecule, has been reported to interact with salivary components on the tooth surface. it might be a candidate antigen inducing the production of antibodies against the adherence of s. mutans to the tooth surface. we investigated the effects of monoclonal antibodies (moabs) obtained by immunization of synthetic pac peptides that completely correspond to the amino acid sequence of part of the a-region. these ...	2001	11439156