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bartonella seropositivity in children with henoch-schonlein purpura.an association between henoch-schonlein purpura (hsp) and seropositivity for bartonella henselae (bh) has been described. the objective of this study was to see if such an association exists in northern alberta.200515807903
[bartonella and bartonellosis--emerging and re-emerging infections: epidemiology, clinical course, diagnosis].information on the epidemiology, clinic and diagnostics of bartonellosis is updated. the importance of bartonellosis lies in the fact that its main risk group embraces patients with immunodeficiencies of different origin, the number of such patients constantly growing. the diagnostics of bartonellosis is insufficiently developed and the research on bartonella organisms, except for the trunch (volynia) fever causative agent, is insufficient in our country.200515773411
[non infectious endocarditis: retrospective study (6 cases)].if there is cardiac valve vegetation and the blood cultures are negatives we need to look for slow growing bacteria, fungi, legionella pneumophilia, bartonella henselae and quintana, brucella melitensis and abortus, coxiella burneti, chlamydiae pneumoniae by serologic tests. the diagnosis of non- infectious endocarditis could be considered only if these results were negative. the main purpose of this study was to describe the clinical and echocardiographic signs of non-infectious endocarditis ca ...200515777581
multifocal bone marrow involvement in cat-scratch disease.we describe 2 children with prolonged fever of unknown origin and prominent skeletal pain who had multifocal bone disease caused by bartonella infection. initial radiologic studies, including plain films, radionuclide scintigraphy and computed tomography, yielded negative results. in both cases, magnetic resonance imaging revealed multiple enhancing bone marrow lesions consistent with clinical symptoms. microbiologic diagnoses were established serologically.200515876956
autochthonous epidemic typhus associated with bartonella quintana bacteremia in a homeless person.trench fever, a louse-borne disease caused by bartonella quintana, is reemerging in homeless persons. epidemic typhus is another life-threatening louse-borne disease caused by rickettsia prowazekii and known to occur in conditions of war, famine, refugee camps, cold weather, poverty, or lapses in public health. we report the first case of seroconversion to r. prowazekii in a homeless person of marseilles, france. this was associated with b. quintana bacteremia. although no outbreaks of typhus ha ...200515891141
[diseases produced by bartonella].the genus bartonella is cause of a broad number of emerging and re-emerging infectious diseases. clinical manifestations depend on the implicated bartonella sp. and the immunity of the host. because there is not a universal therapy for this infection, treatment should be chosen individually. bartonella sp. is responsible of potentially serious clinical pictures (endocarditis, chronic bacteremia, bacillary angiomatosis, carrion's disease, etc.), so clinical suspicion, a quick diagnosis and an ear ...200515899181
seroprevalence of antibodies against bartonella species and evaluation of risk factors and clinical signs associated with seropositivity in dogs.to determine the seroprevalence of antibodies against bartonella spp in a population of sick dogs from northern california and identify potential risk factors and clinical signs associated with seropositivity.200515900951
evidence of bartonella sp. in questing adult and nymphal ixodes ricinus ticks from france and co-infection with borrelia burgdorferi sensu lato and babesia sp.ticks are known vectors for a wide range of pathogenic microorganisms. their role in the transmission of some others is so far only suspected. ticks can transmit multiple pathogens, however, little is known about the co-existence of these pathogens within questing ticks. we looked for the presence of dna from three micro-organisms, bartonella sp., borrelia burgdorferi sensu lato and babesia sp. which are known or suspected tick-borne pathogens, using a cohort of 92 questing ixodes ricinus ticks ...200515610725
demonstration of intracellular microorganisms (rickettsia spp., chlamydia pneumoniae, bartonella spp.) in pathological human aortic valves by pcr.rickettsiae, which causes vasculitis, has not been linked to atherosclerotic cardiovascular disease in contrast to chlamydia pneumoniae whose association with coronary artery disease and with sclerotic heart valves in patients undergoing aortic valve replacement is well established, even if causality is yet to unproven. in the search for any of these infectious agents, 84 pathological and 15 normal aortic heart valves of patients undergoing forensic autopsy were analysed by pcr and dna-sequencin ...200515603840
a review of emerging flea-borne bacterial pathogens in new zealand.recent studies have shown that rickettsia typhi, r. felis, bartonella henselae, and b. clarridgeiae occur in new zealand. to raise awareness of these emerging and re-emerging flea-borne bacterial pathogens among new zealand health workers, we review the clinical features, diagnosis, and treatment of infections, and the biology of the major flea vectors.200515682209
[cat-scratch disease]. 200515695034
a bipartite signal mediates the transfer of type iv secretion substrates of bartonella henselae into human cells.bacterial type iv secretion (t4s) systems mediate the transfer of macromolecular substrates into various target cells, e.g., the conjugative transfer of dna into bacteria or the transfer of virulence proteins into eukaryotic host cells. the t4s apparatus virb of the vascular tumor-inducing pathogen bartonella henselae causes subversion of human endothelial cell (hec) function. here we report the identification of multiple protein substrates of virb, which, upon translocation into hec, mediate al ...200515642951
ectoparasitism and vector-borne diseases in 930 homeless people from marseilles.homeless people are particularly exposed to ectoparasites, but their exposure to arthropod-borne diseases has not been evaluated systematically. a medical team of 27 persons (7 nurses, 6 infectious disease residents or fellows, 2 dermatologists, and 12 infectious disease specialists) visited the 2 shelters in marseilles, france, for 4 consecutive years. homeless volunteers were interviewed, examined, and received care; and blood was sampled for cell counts and detection of bacteremia, antibodies ...200515643300
visual loss after cat scratch. 200515632237
bartonella henselae inhibits apoptosis in mono mac 6 cells.bartonella henselae causes the vasculoproliferative disorders bacillary angiomatosis and peliosis probably resulting from the release of vasculoendothelial growth factor (vegf) from infected epithelial or monocytic host cells. here we demonstrate that b. henselae in addition to vegf induction was also capable of inhibiting the endogenous sucide programme of monocytic host cells. our results show that b. henselae inhibits pyrrolidine dithiocarbamate (pdtc)-induced apoptosis in mono mac 6 cells. b ...200515617526
[cotton wool spots in a 16-year-old girl]. 200515095109
disseminated cat scratch disease with vertebral osteomyelitis and epidural abscess.a 5-year-old boy with cat scratch disease presented with fever of unknown origin and osteomyelitis of the thoracic spine and epidural abscess. he did not have localizing signs or symptoms. computed tomography of the abdomen, which was initially negative, showed hepatosplenic disease. cat scratch disease has variable systemic presentations and should be included in the differential diagnosis of fever of unknown origin if an epidemiologic risk factor is present.200516351038
pattern visual evoked potentials in eyes with disc swelling due to cat scratch disease-associated neuroretinitis.to evaluate optic nerve function by pattern visual evoked potentials (veps) in eyes with optic disc swelling due to neuroretinitis associated with cat scratch disease (csd).200516328936
aortic valve endocarditis with bartonella quintana - a rare entity.endocarditis caused by bartonella species is difficult to diagnose and still remains a rare entity. therefore, a young male patient undergoing aortic valve replacement for culture-negative endocarditis is reported in whom the diagnosis of a bartonella quintana infection was made with a great delay postoperatively.200516331372
[detection of the bartonella dna by the method of nested pcr in patients after tick bites in novosibirsk region].arthropod-borne bacterial pathogen bartonella dna was detected in human blood cells after tick bites in summer 2003 and 2004 in novosibirsk region by nested pcr with primers specific to groel gene of hsp60 protein. comparative assay of 190 p.b. of long pcr fragment revealed that the nucleotide sequences might belong to bartonella henselae and bartonella quintana.200516334219
[cat-scratch disease as cause of lymphadenitis colli].cat-scratch disease as cause of lymphadenitis colli. cat-scratch disease is a frequent cause of lymphadenitis colli. it mainly affects children and adolescents younger than 21 years. since the clinical picture is not characteristic, a history of contact to cats or kittens is highly valuable for diagnosing the disease. major aspects of the disease concerning epidemiology, diagnostic procedures, clinical presentation and therapy are discussed.200516358204
comparative proteomic analysis of b. henselae houston and b. henselae marseille by two-dimensional gel electrophoresis.to compare the protein difference between b. henselae houston and b. henselae marseille by two-dimensional gel electrophoresis.200516370318
[prevalence of serum antibodies against bartonella ssp. in a health population from the south area of the seville province].to know the prevalence of serum antibodies against bartonella spp. in a healthy population from south of spain.200516381097
[multifocal chorioretinitis, papillitis, and recurrent optic neuritis in cat-scratch disease].to evaluate the causative factor for multifocal chorioretinitis, papillitis, and recurrent optic neuritis. a 41-year-old patient presenting multifocal choroiditis, papillitis, and recurrent optic neuritis was evaluated with funduscopy, angiography (fa), optical coherence tomography (oct), visual evoked potentials (vep), and numerous blood laboratory tests. fa and oct showed multifocal pigment epithelial detachments. vep showed typical changes for optic neuritis and papillitis. indirect fluoresce ...200516395190
[abrupt visual loss in children. three case studies of ocular bartonellosis].to evaluate the severity and progression of neuroretinitis in children caused by bartonella.200516395224
bartonella henselae in porpoise blood.we report detection of bartonella henselae dna in blood samples from 2 harbor porpoises (phocoena phocoena). by using real-time polymerase chain reaction, we directly amplified bartonella species dna from blood of a harbor porpoise stranded along the northern north carolina coast and from a pre-enrichment blood culture from a second harbor porpoise. the second porpoise was captured out of habitat (in a low-salinity canal along the northern north carolina coast) and relocated back into the ocean. ...200516485476
bartonella quintana in cynomolgus monkey (macaca fascicularis).we identified a bartonella quintana strain by polymerase chain reaction amplification, cloning, and sequencing of dna extracted from lysed erythrocytes and cultured colonies grown from peripheral blood collected from a captive-bred cynomolgus monkey (macaca fascicularis). this report describes naturally acquired b. quintana infection in a nonhuman primate.200516485482
bartonella and rickettsia in fleas and lice from mammals in south carolina, u.s.a.species in the genera bartonella and rickettsia are vector-borne pathogens of humans and domestic animals. the natural reservoirs and enzootic transmission cycles of these bacteria are poorly known in south carolina. thirteen species of lice and fleas were collected from urban animals and screened for the presence of bartonella and rickettsia by pcr amplification using genus-specific primers. bartonella henselae was present in cat fleas (ctenocephalides felis) from virginia opossums (didelphis v ...200516599169
molecular evidence for novel bartonella species in trichobius major (diptera: streblidae) and cimex adjunctus (hemiptera: cimicidae) from two southeastern bat caves, u.s.a. 200516599175
bartonella species in rodents and shrews in the greater jakarta area.in february 2004, we captured 221 rodents and shrews in the greater jakarta area as part of a study to determine the prevalence of rodent-associated vector-borne infections. microscopic examination of blood smears revealed 6% (13/218) to be positive for bartonella spp. the corresponding dna samples, either from blood blots or frozen spleen pieces and from fleas collected on these animals, were tested for evidence of bartonella infection by pcr, targeting the portions: 378bp and 930bp of the citr ...200516610656
[diagnostics of human granulocytic anaplasmosis].diagnostics of human granulocytic anaplasmosis. human granulocytic anaplasmosis is one of recently emerging tick-borne diseases. the paper summarizes current opinions on diagnostics of this zoonosis, including the recent recommendations of escar (european society of clinical microbiology and infectious diseases study group on coxiella, anaplasma, rickettsia and bartonella).200516838619
[capreolus capreolus and ixodes ricinus as a reservoir of bartonella in north-western poland].capreolus capreolus and ixodes ricinus as a reservoir of bartonella in north-western poland. the purpose of the study was to assess the prevalence bartonella in capreolus capreolus from north-western poland forest. supplementary, ticks infesting roe deer were also screened in order to ascertain their role as vectors and reservoirs of bartonella. the samples of blood from 98 animals from north-western poland were pcr-screened. bartonella dna was detected by using primers complementary to the inte ...200516838623
bacillary angiomatosis in an hiv-positive man with multiple risk factors: a clinical and epidemiological puzzle.a 36-year-old man with advanced aids presented with skin lesions, fever and weight loss. physical examination revealed skin lesions suggestive of bacillary angiomatosis. diagnostic imaging identified bone lesions, soft tissue masses and a single hepatic lesion. his clinical presentation was most consistent with bartonella quintana; however, his risk for infection with b quintana was limited to a remote, brief period of homelessness. in contrast, his frequent contact with cats presented substanti ...200518159553
[fleas and diseases transmissible to man].fleas are wingless insects that parasitize mammals and more rarely birds. they are able to jump and may bite people. adult fleas are hematophagous. the impact of fleas on public health is related to their ability to act as vectors for transmission of infectious agents during blood meals. the purpose of this article is to describe fleas and the diseases that they transmit to humans. special focus is placed on epidemiological aspects.200616615611
bacillary angiomatosis associated with pseudoepitheliomatous hyperplasia.bacillary angiomatosis is an opportunistic bacterial infection caused by either bartonella henselae or b. quintana. the classic histologic presentation of bacillary angiomatosis involves three components: a lobular proliferation of capillaries with enlarged endothelial cells, neutrophilic debris, and clumps of finely granular material identified as bacteria with staining techniques. pseudoepitheliomatous hyperplasia is a histologic reaction pattern characterized by epithelial proliferation in re ...200616456322
ocular manifestation of cat-scratch disease in hiv-positive patients.to characterize ocular manifestations of cat-scratch disease in hiv-positive patients.200616458711
antimicrobial susceptibility of bartonella henselae using etest methodology.bartonella henselae is a fastidious slow growing pathogen which is seldom cultured in the laboratory. previous descriptions of antimicrobial susceptibility have been largely limited to feline isolates and/or laboratory reference strains, with no accounting for genotypic or phenotypic diversity.200616464897
parotid mass due to cat scratch disease.cat scratch disease (csd), due to bartonella henselae, is a self-limited chronic lymphadenopathy. a previously healthy 22-year-old woman presented with a palpable painful swelling in the right submandibular region accompanied by enlarged cervical lymph nodes. a diagnosis of b. henselae infection was made according to her personal history that divulged frequent contacts with cats and to a high titre of immunoglobulin g (igg) and igm antibodies for this agent. the patient improved within 1 month w ...200616669837
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
secondary unilateral glaucoma and neuroretinitis: atypical manifestation of cat-scratch disease. 200616604397
bartonella quintana associated neuroretinitis.we report an observational case of bartonella quintana-associated neuroretinitis. the patient had a positive igm ifa titer for bartonella quintana early in the disease. after treatment, the neuroretinitis and igm resolved. given the patient's history, symptoms, response to treatment, and igm course, we believe his neuroretinitis was secondary to bartonella quintana.200616449005
effects of cow age and pregnancy on bartonella infection in a herd of dairy cattle.bartonella spp. are small hemotropic bacteria infecting mammals. four bartonella species have been recently described in cattle and wild ruminants. to date, the biology and possible pathogenic role of bartonella species isolated from ruminants are poorly understood. therefore, a dairy herd of 448 cows and heifers was surveyed in order to establish the prevalence of bartonella bovis and b. chomelii infections, the level of bacteremia, and the relationship between bacteremia and age or pregnancy s ...200616390945
bartonella quintana characteristics and clinical management.bartonella quintana, a pathogen that is restricted to human hosts and louse vectors, was first characterized as the agent of trench fever. the disease was described in 1915 on the basis of natural and experimental infections in soldiers. it is now recognized as a reemerging pathogen among homeless populations in cities in the united states and europe and is responsible for a wide spectrum of conditions, including chronic bacteremia, endocarditis, and bacillary angiomatosis. diagnosis is based on ...200616494745
first isolation of bartonella alsatica from a valve of a patient with endocarditis.we report the first documented case of endocarditis in a man infected with bartonella alsatica, which causes bacteremia in healthy wild rabbits. b. alsatica was identified by serology and culture and by pcr of an aortic valve specimen. b. alsatica should be added to the list of zoonotic agents of blood culture-negative endocarditis.200616390990
localized bacillary angiomatosis in the oral cavity: observations about a neoplasm with atypical behavior. description of a case and review of the literature.bacillary angiomatosis is a rather frequent infectious pathology appearing mainly in the skin but can also affect the liver, spleen, heart, bones, lungs, muscles, central nervous system and other organs. the localization of the lesion in the oral cavity is rather rare, as it is evident in the literature. bacillary angiomatosis can be clinically similar to the kaposi's sarcoma and histologically confused with angiosarcoma, epitheloid hemangioma and pyogenic granuloma. a case of bacillary angiomat ...200616495874
purification of the yada membrane anchor for secondary structure analysis and crystallization.non-fimbrial adhesins, such as yersinia yada, moraxella uspa1 and a2, haemophilus hia and hsf, or bartonella bada, represent an important class of molecules by which pathogenic proteobacteria adhere to their hosts. they form trimeric surface structures with a head-rod-anchor architecture. whereas their head and rod domains may be of heterologous origin, their anchor domains are homologous and display the properties of autotransporters. conflicting topology models exist for these membrane anchors ...200616405993
brucella outer membrane protein omp31 is a haemin-binding protein.the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ...200616517201
kikuchi's disease or kikuchi's syndrome? 200616421809
transient monoclonal gammopathy in a patient with bartonella quintana endocarditis.monoclonal gammopathy has been reported rarely in association with infectious diseases. viral infection has been the most frequently reported. we report a case of bartonella quintana endocarditis in a 45-year-old homeless male associated with a monoclonal igg kappa gammopathy. the gammopathy disappeared after 8 months of antibiotics while the bartonella antibody titre was decreasing. this correlation suggests a causative role for b. quintana for the monoclonal gammopathy. to the best of our know ...200616432867
etiology of tick-borne febrile illnesses in adult residents of north-eastern poland: report from a prospective clinical study.febrile tick-borne diseases can be caused by different pathogens. the study objective was to prospectively determine the etiology of infection among adults exposed to tick bite and to detect acute human granulocytic anaplasmosis (hga), a recently emerging infection, in north-eastern poland. we evaluated 68 patients (34 males and 34 females, mean age 44.2+/-15 years) who had fever within 4 weeks after a tick bite. paired sera were tested for antibodies against tick-borne encephalitis virus, borre ...200616530481
serological survey of vector-borne zoonotic pathogens in pet cats and cats from animal shelters and feral colonies.although cats and their arthropod parasites can sometimes be important sources of zoonotic diseases in humans, the extent of exposure among various cat populations to many potential zoonotic agents remains incompletely described. in this study, 170 domestic cats living in private homes, feral cat colonies, and animal shelters from california and wisconsin were evaluated by serology to determine the levels of exposure to a group of zoonotic vector-borne pathogens. serological positive test result ...200616434226
confirmation of diagnosis of cat scratch disease by immunohistochemistry. 200616434905
pet ownership in immunocompromised children--a review of the literature and survey of existing guidelines.pet ownership has been associated with both emotional and physical health benefits. however, owning pets may also pose health risks to immunocompromised patients through zoonotic transmission of disease. our initial impression was that there is a lack of any evidence base in information given by health care professionals regarding these risks. we therefore aimed to produce evidence-based guidelines addressing this issue. a pubmed search was undertaken and a variety of literature on zoonoses revi ...200616581294
prevalence of selected infectious disease agents in cats from arizona.the objective of this study was to use polymerase chain reaction (pcr) assays to determine the prevalence of ehrlichia species, anaplasma phagocytophilum, mycoplasma haemofelis, 'candidatus mycoplasma haemominutum' and bartonella species from feral and relinquished cats in phoenix and nogales, arizona. dna from one or more of the organisms was amplified from 31 of 112 blood samples (27.7%). dna consistent with bartonella clarridgeiae 15 (13.4%), bartonella henselae 14 (12.5%), 'candidatus m haem ...200616443383
antibodies to rickettsia rickettsii, rickettsia typhi, coxiella burnetii, bartonella henselae, bartonella quintana, and ehrlichia chaffeensis among healthy population in minas gerais, brazil.rickettsial diseases except those belonging to spotted fever group rickettsioses are poorly studied in south america particularly in brazil where few epidemiological reports have been published. we describe a serosurvey for rickettsia rickettsii, r. typhi, coxiella burnetii, bartonella henselae, b. quintana, and ehrlichia chaffeensis in 437 healthy people from a brazilian rural community. the serum samples were tested by indirected micro-immunoflourescence technique and a cutoff titer of 1:64 wa ...200616444416
[presence of bartonella henselae in cats: natural reservoir quantification and human exposition risk of this zoonoses in chile].the availability of a serologic test for cat scratch disease in humans has allowed the diagnosis of an increasing number of cases of this disease in chile.200616446874
bartonella quintana endocarditis with positive serology for coxiella burnetii.both bartonella quintana and coxiella burnetii are known to cause of blood culture negative endocarditis. in such case, the diagnosis is usually established by serology. a case of bartonella quintana endocarditis is described where the serology was falsely positive for coxiella burnetii. this case demonstrates the difficulty in distinguishing these two etiologic agents by routine serologic testing.200616386308
antiangiogenic effect of erythromycin: an in vitro model of bartonella quintana infection.bartonella quintana, the etiological agent of bacillary angiomatosis (ba), causes endothelial cell proliferation. erythromycin has dramatic effects on ba, and the effects are largely unexplained by the compound's bacteriostatic properties. our aim here was to evaluate the possibility that erythromycin alters angiogenesis.200616388485
antibodies to bartonella vinsonii subsp. berkhoffii in moroccan dogs.bartonella vinsonii subsp. berkhoffii is a fastidious microorganism that has been recognized as an emerging human and canine pathogen. we report for the first time on the prevalence of antibodies to b. vinsonii subsp. berkhoffii in domestic dogs from morocco. the overall seroprevalence was 38% (56 of 147 dogs tested). most of the seropositive dogs were stray dogs from rabat (36%, 8 of 22) and khenifra (47%, 47 of 101). antibodies against b. vinsonii subsp. berkhoffii were found infrequently amon ...200616474074
a bartonella vinsonii berkhoffii typing scheme based upon 16s-23s its and pap31 sequences from dog, coyote, gray fox, and human isolates.since the isolation of bartonella vinsonii subspecies berkhoffii from a dog with endocarditis in 1993, this organism has emerged as an important pathogen in dogs and as an emerging pathogen in people. current evidence indicates that coyotes, dogs and gray foxes potentially serve as reservoir hosts. based upon sequence differences within the 16s-23s its region and pap31 gene, we propose a classification scheme that divides b. vinsonii subsp. berkhoffii isolates into four distinct types. two conse ...200616460911
plasmacytoid monocytes in cat scratch disease with special reference to the histological diversity of suppurative lesions.it has been suggested that plasmacytoid monocytes (pmos) play an essential role in t-cell-dependent immune response. indeed, numerous pmos are found in close topographical association with epithelioid cell granulomas in hypersensitivity-type granulomas, such as tuberculosis and sarcoidosis. the key pathologic process in cat scratch disease (csd) usually involves a b-cell-associated granulomatous reaction. histologically, csd appears to exhibit a histopathologic diversity, including suppurative l ...200616352403
bartonella quintana, lice, and molecular tools. 200617017209
[cat-scratch disease. review of eight adult patients hospitalized for fever or adenopathy].cat-scratch disease is common among children. among adults the disease is less often considered in the differential diagnosis of enlarged lymph nodes and fever.200617186093
dynamics of the incw genetic backbone imply general trends in conjugative plasmid evolution.plasmids cannot be understood as mere tools for genetic exchange: they are themselves subject to the forces of evolution. their genomic and phylogenetic features have been less studied in this respect. focusing on the incw incompatibility group, which includes the smallest known conjugative plasmids, we attempt to unveil some common trends in plasmid evolution. the functional modules of incw genetic backbone are described, with emphasis on their architecture and relationships to other plasmid gr ...200617026718
molecular detection of rickettsia felis, rickettsia typhi and two genotypes closely related to bartonella elizabethae.a total of 56 fleas were collected from mice, rats, and one hedgehog in national parks of mainland portugal and the madeira island. all fleas were tested for the presence of bacteria of the genera rickettsia and bartonella using pcr assays. in fleas from mainland portugal, we detected rickettsia felis in one archaeopsylla erinacei maura flea and in one ctenophtalmus sp. in five leptopsylla segnis fleas taken from rats in the madeira island, we identified rickettsia typhi. in addition, in four fl ...200617038702
the reach of the genome signature in prokaryotes.with the increased availability of sequenced genomes there have been several initiatives to infer evolutionary relationships by whole genome characteristics. one of these studies suggested good congruence between genome synteny, shared gene content, 16s ribosomal dna identity, codon usage and the genome signature in prokaryotes. here we rigorously test the phylogenetic signal of the genome signature, which consists of the genome-specific relative frequencies of dinucleotides, on 334 sequenced pr ...200617040564
isolated hepatic involvement of cat scratch disease in immunocompetent adults: enhanced magnetic resonance imaging, pathological findings, and molecular analysis--two cases.visceral involvement in absence of lymphadenopathy is a rare manifestation in cat scratch disease; hepatic granulomas are rare, representing 0.3% of systemic manifestations of cat scratch disease, and gallbladder extension is a singular case. the present article refers to 2 rare cases of visceral cat scratch disease in immunocompetent adults with hepatic granulomatous inflammation, caused by bartonella henselae infection, with gallbladder involvement in 1 case and no lymphadenopathy. histologica ...200617041206
the low seroprevalence of tick-transmitted agents of disease in dogs from southern ontario and quebec.infectious diseases caused by pathogens transmitted by ticks and other insect vectors are an important cause of morbidity and mortality in both dogs and humans throughout north america. the purpose of this study was to determine the seroprevalence of selected vector-transmitted pathogens in southern ontario and quebec. samples submitted to the vector borne disease diagnostic laboratory (vbddl) at the north carolina state university college of veterinary medicine were evaluated for antibodies to ...200617217089
lymph node biopsy specimens and diagnosis of cat-scratch disease.we report microbiologic analysis of 786 lymph node biopsy specimens from patients with suspected cat-scratch disease (csd). the specimens were examined by standard, cell culture, and molecular methods. infectious agents were found in samples from 391 (49.7%) of 786 patients. the most commonly identified infectious agent was bartonella henselae (245 patients, 31.2%), the agent of csd. mycobacteriosis was diagnosed in 54 patients (6.9%) by culture and retrospectively confirmed by using a specific ...200617073081
[wild small mammals are the reservoir hosts of the bartonella genus bacteria in the south of moscow region].a total of 103 blood samples collected from wild small mammals captured in the prioksko-terrasny reserve on the south of moscow region were studied to determine the bartonellae prevalence. the examined species were the yellow-necked mice apodemus flavicollis (35 samples), the european wood mouse apodemus uralensis (10 samples), the bank vole clethrionomys glareolus (51 samples), the house mouse mus musculus (3 samples), the common vole microtus arvalis (2 samples), and the shrew sorex araneus (2 ...200617094651
indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following listeria monocytogenes infection.control of pathogens by formation of abscesses and granulomas is a major strategy of the innate immune system, especially when effector mechanisms of adaptive immunity are insufficient. we show in human listeriosis that dcs expressing indoleamine 2,3-dioxygenase (ido), together with macrophages, are major cellular components of suppurative granulomas in vivo. induction of ido by dcs is a cell-autonomous response to listeria monocytogenes infection and was also observed in other granulomatous inf ...200617111046
bartonella henselae endocarditis in a child.14-year-old white male with a past medical history of congenital bicuspid aortic valve, streptococcus viridans endocarditis, and pulmonary valve homograft presented with culture-negative endocarditis. molecular studies identified the causative organism as bartonella henselae and subsequent serologic studies supported this diagnosis. this rare cause of endocarditis may be under recognized in children. bartonella henselae endocarditis should be considered in all children with culture-negative endo ...200617111290
infective endocarditis due to bartonella spp. and coxiella burnetii: experience at a cardiology hospital in sao paulo, brazil.bartonella spp. and coxiella burnetii are recognized as causative agents of blood culture-negative endocarditis (bcne) in humans and there are no studies of their occurrences in brazil. the purpose of this study is to investigate bartonella spp. and c. burnetii as a causative agent of culture-negative endocarditis patients at a cardiology hospital in são paulo, brazil. from january 2004 to december 2004 patients with a diagnosis of endocarditis at our institute were identified and recorded prosp ...200617114712
arthropod-borne diseases in homeless.homeless people are particularly exposed to ectoparasite. the living conditions and the crowded shelters provide ideal conditions for the spread of lice, fleas, ticks, and mites. body lice have long been recognized as human parasites and although typically prevalent in rural communities in upland areas of countries close to the equator, it is now increasingly encountered in developed countries especially in homeless people or inner city economically deprived population. fleas are widespread but ...200617114713
prevalence of rickettsia felis-like and bartonella spp. in ctenocephalides felis and ctenocephalides canis from la rioja (northern spain).our aim was to determine the presence of rickettsia spp. and bartonella spp. in ctenocephalides felis and ctenocephalides canis from la rioja (spain). a total of 88 specimens were tested by polymerase chain reaction (pcr) using glta and ompb genes as targets for rickettsia spp., and 16s rrna and ribc genes for bartonella spp. rickettsia felis-like (28.4%), bartonella clarridgeiae (6.8%), and bartonella henselae (3.4%) were detected in ctenocephalides spp. other bartonella sp. different from b. c ...200617114721
american association of feline practitioners 2006 panel report on diagnosis, treatment, and prevention of bartonella spp. infections. 200616846781
first evidence of feline herpesvirus, calicivirus, parvovirus, and ehrlichia exposure in brazilian free-ranging felids.serum samples from 18 pumas (puma concolor), one ocelot (leopardus pardalis), and two little spotted cats (leopardus tigrinus) collected from free-ranging animals in brazil between 1998 and 2004 were tested by indirect immunofluorescence (ifa) for antibodies to feline herpesvirus 1 (fhv 1), calicivirus (fcv), coronavirus (fcov), parvo-virus (fpv), ehrlichia canis, anaplasma pha-gocytophilum, and bartonella henselae. serum samples also were tested, by western blot and elisa, for feline leukemia v ...200616870878
cloning, characterization, and expression of bartonella henselae p26.in order to identify immunoreactive bartonella henselae proteins, b. henselae antiserum from an experimentally infected cat was used to screen a b. henselae genomic dna expression library. one immunoreactive phage clone contained a gene (p26) with significant nucleotide identity with orthologs in brucellae, bartonellae, and several plant-associated bacteria. p26 gene sequences from four b. henselae strains, one b. koehlerae strain, and one b. clarridgeiae strain were cloned. comparative nucleoti ...200616893981
broad-range polymerase chain reaction for the diagnosis of bartonella henselae endocarditis.we present a case of subacute bacterial endocarditis in a 10-year-old girl with di-george syndrome, congenital heart disease, and mild immunodeficiency. she was afebrile at initial presentation but was found to have massive splenomegaly, and signs of congestive heart failure. no causative organism could be identified on routine blood and tissue cultures. a detailed clinical history revealed a history that she had been scratched by a cat and developed intermittant fevers over 3 months. bartonella ...200616898887
ixodes frontalis and avian tick-related syndrome in the united kingdom.this study aimed to characterise tick species responsible for avian tick infestations in the uk, to analyse various risk factors for tick-related syndrome in tick-infested birds and to test samples for the presence of certain tick-transmitted pathogens.200616911113
in vitro susceptibility of bartonella species to 17 antimicrobial compounds: comparison of etest and agar dilution.in vitro susceptibility testing of 31 bartonella spp. strains including 21 bartonella henselae isolates was performed for 17 antimicrobial agents (telithromycin, four macrolides, five fluoroquinolones, five aminoglycosides, doxycycline and rifampicin).200616916864
serological and molecular evidence of exposure to arthropod-borne organisms in cats from northeastern spain.one hundred sixty-eight cat sera from spain were tested for igg antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap) and bartonella henselae (bh) antigens using ifa and for felv antigen and fiv antibody by elisa. for 47 whole blood samples, pcr testing was performed for rickettsia, ehrlichia and bartonella. seroprevalences were: bh (71.4%), rc (44%), ec (11.3%), felv (8.5%), fiv (7.4%) and ap (1.8%). bh antibodies were associated with seroreactivity to both ...200616919405
bartonella quintana variably expressed outer membrane proteins mediate vascular endothelial growth factor secretion but not host cell adherence.bartonella quintana causes trench fever, endocarditis, and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. little is known about the interaction of this pathogen with host cells. we attempted to elucidate the interaction of b. quintana with human macrophages (thp-1) and epithelial cells (hela 229). remarkably, only b. quintana strain jk-31 induced secretion of vascular endothelial growth factor (vegf) from thp-1 and hela 229 cells upon infection similar ...200616926391
autocrine role for interleukin-8 in bartonella henselae-induced angiogenesis.the gram-negative bacterium bartonella henselae is capable of causing angiogenic lesions as a result of infection. previously, it has been shown that b. henselae infection can result in production of the chemokine interleukin-8 (il-8). in this study, we demonstrated that monocytes, endothelial cells, and hepatocytes produce il-8 in response to b. henselae infection. we also investigated the role of il-8 in b. henselae-induced endothelial cell proliferation and capillary tube formation. both in v ...200616926411
systemic cat scratch disease.systemic cat scratch disease (csd) is often associated with prolonged fever and microabscesses in the liver and/or spleen. we report a case of systemic csd with hepatic, splenic and renal involvement in an aboriginal child in taiwan. a previously healthy 9-year-old girl had an intermittent fever for about 17 days, and complained of abdominal pain, headache and weight loss. abdominal computed tomography showed multiple tiny hypodense nodular lesions in the spleen and both kidneys. laparotomy reve ...200616935770
case records of the massachusetts general hospital. case 27-2006. a 17-year-old boy with fever and lesions in the liver and spleen. 200616943407
analysis of data in 30 patients with cat scratch disease without lymphadenopathy.the prominent clinical manifestation of cat scratch disease is regional lymphadenopathy at the site of the cat scratch or bite, associated with fever or general symptoms. a serological study of 540 patients with either lymphadenopathy, persistent fever, or pet ownership disclosed that 30 (16.1%) of the 186 patients with a serological diagnosis of cat scratch disease had no regional lymphadenopathy, and in these 30 patients, the absence of lymphadenopathy was closely related to the presence of pe ...200616944264
prevalence of bartonella henselae antibodies in serum of cats with and without clinical signs of central nervous system disease.bartonella henselae is occasionally associated with neurological dysfunction in people and some experimentally infected cats. the purpose of this study was to determine whether b henselae seroprevalence or titer magnitude varies among cats with neurological disease, cats with non-neurological diseases, and healthy cats while controlling for age and flea exposure. there was no difference in b henselae seroprevalence rates between cats with seizures and cats with other neurological diseases. cats ...200616949848
a translocated bacterial protein protects vascular endothelial cells from apoptosis.the modulation of host cell apoptosis by bacterial pathogens is of critical importance for the outcome of the infection process. the capacity of bartonella henselae and b. quintana to cause vascular tumor formation in immunocompromised patients is linked to the inhibition of vascular endothelial cell (ec) apoptosis. here, we show that translocation of bepa, a type iv secretion (t4s) substrate, is necessary and sufficient to inhibit ec apoptosis. ectopic expression in ecs allowed mapping of the a ...200617121462
[endocarditis due to bartonella spp. three new clinical cases and spanish literature review]. 200617125688
[a case of hepatosplenic cat scratch disease].a 43-year-old man was admitted with idiopathic fever. abdominal ultrasonogram demonstrated multiple hypoechoic lesions in the spleen. abdominal ct scan showed multiple hypodense lesions in the liver and spleen. the patient had a cat in his house, and the presence of a very high serous antibody titer for bartonella henselae led to the diagnosis of hepatosplenic cat scratch disease. it is important to consider this disease in the differential diagnosis of idiopathic fever when multiple lesions are ...200616953102
transient paresis associated with cat-scratch disease: case report and literature review of vertebral osteomyelitis caused by bartonella henselae.cat-scratch disease (csd) rarely presents as vertebral osteomyelitis. we describe a case with paresis of the arm with total recovery after antibiotic and neurosurgical therapy. we reviewed 20 other cases of csd vertebral osteomyelitis in the literature. this diagnosis should be considered in patients with systemic symptoms, back pain, and cat contact. the prognosis is generally good.200617133166
bartonella quintana prosthetic valve endocarditis detected by blood culture incubation beyond 10 days.we describe the case of a 45-year-old man with mitral and aortic prosthetic valve replacement who presented with symptoms of subacute bacterial endocarditis. bartonella quintana was grown from blood after prolonged culture. the course of the disease was complicated by splenic infarction, glomerulonephritis resulting in progressive renal insufficiency, and cerebroventricular hemorrhage. notably, cardiac ultrasonography showed no extensive vegetations but a strand-like lesion. culture-positive b. ...200616962956
[bacterial zoonoses: emerging concepts].bacterial zoonoses are evolving with changes in society, climate and lifestyles. a hierarchy of non food-borne zoonoses was recently proposed in france, and includes characteristics such as severity criteria and bioterrorism potential. the creation of specific networks and reference centers has provided the means to monitor the emergence (or re-emergence) of zoonoses such as brucellosis and q fever. molecular tools have facilitated the detection of bacteria that are transmitted by arthropod vect ...200617140097
rickettsial agents in egyptian ticks collected from domestic animals.to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ...200617004028
[bartonella henselae as a cause of optical nerve neuritis].bartonella henselae is included into the group of gram-negative bacteria that can cause not so rare disease known as cat-scratch disease (csd). this disease is characterized by the specific general symptoms, and the complications in the eyes can be manifested in the form of neuroretinitis, follicular conjunctivitis and focal chorioretinitis. in this paper clinical and epidemiological characteristics of a patient with ophthalmologic complications caused by bartonella henselae are described. we in ...200617144434
an unusual outcome in a child with hepatosplenic cat-scratch disease.typical cat-scratch disease (bartonella henselae infection) in an immunocompetent child is usually associated with a history of scratch, bite or intimate contact with a cat. most patients develop a non-tender papule in the scratch line after three to ten days. this may persist for only a few days or as long as two to three weeks. during the next two weeks or more, regional lymph nodes that drain the area gradually enlarge and then slowly resolve in more than 10% of patients. the nodes develop ov ...200617136337
serologic survey of eptesicus fuscus from georgia, u.s.a. for rickettsia and borrelia and laboratory transmission of a rickettsia by bat ticks.bats and their ectoparasites are associated with bacterial agents of unknown pathogenicity. we tested sera from 56 eptesicus fuscus from georgia against borrelia hermsii, orientia tsutsugamushi, rickettsia conorii, and rickettsia rickettsii. we detected antibodies reactive against a relapsing fever borrelia and spotted fever group rickettsia in 3/56 and 1/56 bats, respectively. we attempted to culture bartonella from the blood of these bats but were unsuccessful. in addition, we fed bat ticks, c ...200617249357
bartonella infections in fleas (siphonaptera: pulicidae) and lack of bartonellae in ticks (acari: ixodidae) from hungary.fleas (95 pulex irritans, 50 ctenocephalides felis, 45 ctenocephalides canis) and ixodid ticks (223 ixodes ricinus, 231 dermacentor reticulatus, 204 haemaphysalis concinna) were collected in hungary and tested, in assays based on pcr, for bartonella infection. low percentages of p. irritans (4.2%) and c. felis (4.0%) were found to be infected. the groel sequences of the four isolates from p. irritans were different from all the homologous sequences for bartonellae previously stored in genbank bu ...200617252927
demographic features of bartonella infections in richardson's ground squirrels (spermophilus richardsonii).the epidemiology of bartonella infections in richardson's ground squirrels (spermophilus richardsonii) was studied at multiple sites in saskatchewan, canada, from 2002 to 2004. the overall prevalence of bartonella infection was 48%. juvenile squirrels were significantly more likely to be infected with bartonella than were adults (58% and 37%, respectively), and juvenile animals also were significantly more likely to have high levels of bacteremia compared to adult animals. prevalence of bartonel ...200617255440
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