Publications

[susceptibilities of clinical isolates to antibacterial agents. a study mainly focused on ofloxacin (the second report). reported by the research group for testing ofloxacin susceptibility on clinical isolates].	susceptibilities of various clinical isolates to ofloxacin (oflx) and other antibacterial drugs were examined at 128 hospital laboratories in 36 prefectures throughout japan between april, 1986 and march, 1987. the results were totalized with an emphasis mainly on oflx and were compared with data obtained in the previous year. in this study, identification and susceptibility tests of the isolates were carried out at each hospital laboratory and the tests were performed according to the 1-dilutio ...	1989	2664255
characterization of putrescine production in nongrowing vibrio parahaemolyticus cells in response to external osmolality.	nongrowing vibrio parahaemolyticus cells rapidly produced putrescine (put) from added arginine when subjected to a low osmotic stress. this phenomenon was characterized in connection with a regulatory mechanism of the responsible enzymes, arginine decarboxylase (adc) and agmatine ureohydrolase (auh). nacl, kcl, licl, sucrose, and glycerol were used as solutes to prepare the resuspending media with various osmolalities. regardless of whether the solutes were electrolytes or non-electrolytes, expo ...	1989	2543889
a biotin-streptavidin amplified enzymeimmunoassay for 13,14-dihydro-15-keto-pgf2 alpha.	as an alternative for radioimmunoassays a new enzyme immunoassay (eia) for the determination of 13,14-dihydro-15-keto pgf2 alpha (pgfm) has been developed. biocytin was linked to pgfm by the n-hydroxysuccinimide method and the product (biocytinyl-pgfm) purified by reversed phase column chromatography. biocytinyl-pgfm was used in the eia as a bridge between the immobilized pgfm-antibody and streptavidin-peroxidase. the absolute sensitivity of the assay was about 160 amol (92% rel. binding) and re ...	1989	2675197
[antibacterial and bactericidal activities of japanese green tea].	we found that extracts of japanese green tea leaves inhibited the growth of various bacteria causing diarrheal diseases. all tea samples tested showed antibacterial activity against staphylococcus aureus, s. epidermidis, vibrio cholerae o1, v. cholerae non o1. v. parahaemolyticus, v. mimicus, campylobacter jejuni and plesiomonas shigelloides. none of the tea samples had any effect on the growth of v. fluvialis, aeromonas sobria, a. hydrophila, pseudomonas aeruginosa, salmonella enteritidis, ente ...	1989	2677434
digit-muscle responses evoked from multiple intracortical foci in monkey precentral motor cortex.	1. the precentral motor cortex, including the anterior bank of the central sulcus of monkey (macaca fuscata), was systemically penetrated with microelectrodes to determine the spatial organization of the microexcitable cortical elements that can produce responses in digit muscles. 2. at 200-microns intervals on each electrode track, low-current intracortical microstimuli were delivered and the muscle responses evoked from four digit muscles were recorded. the responses, obtained with 5, 8, 15, a ...	1989	2681562
purification and characterization of membrane-bound 5'-nucleotidase of vibrio parahaemolyticus.	membrane-bound 5'-nucleotidase from vibrio parahaemolyticus was solubilized and purified using a nonionic detergent, heptyl-beta-d-thioglucoside, and was characterized. this enzyme required mg2+ for activity, maximum activity being observed at 5 and 20 mm mg2+ with amp and atp, respectively, as substrates. of the divalent cations tested, mn2+ and co2+ were able to replace mg2+ partially, whereas ca2+ was ineffective. zinc strongly inhibited the enzyme activity and ni2+ caused partial inhibition. ...	1989	2546926
immunocytochemical localization of glutamate decarboxylase in the mesothoracic ganglion of locusta migratoria.	glutamate decarboxylase immunoreactivity has been located in the thoracic ganglia of the locust, locusta migratoria, using an antiserum raised from rat brain. at the light microscopic level clusters of nerve cell somata as well as nerve fibres were positively labelled by the antiserum. electron microscopy showed that glutamic acid decarboxylase was localized in numerous synaptic terminals.	1989	2686095
mechanisms of resistance in anaerobes and new developments in testing.	anaerobic bacteria currently demonstrate increased resistance to antimicrobial agents, primarily by the production of beta-lactamase. a number of species of bacteroides, most notably those in the bacteroides fragilis group, produce these enzymes. a few species of fusobacterium and clostridium produce beta-lactamase as well. fortunately, this mechanism of resistance is readily overcome by administering beta-lactamase inhibitors coupled with a beta-lactam antibiotic that would otherwise be inactiv ...	1989	2686914
cloning and expression of the 5'-nucleotidase gene of vibrio parahaemolyticus in escherichia coli and overproduction of the enzyme.	the gene encoding the membrane-bound 5'-nucleotidase of vibrio parahaemolyticus was cloned and expressed in escherichia coli. cells of e. coli harboring a plasmid, pnut5, which carries the 5'-nucleotidase gene were able to grow on atp as the sole source of carbon, although the original cells were not. the 5'-nucleotidase activity was detected in whole cells of e. coli harboring pnut5 and in membrane vesicles prepared from these cells. most properties of the 5'-nucleotidase produced in e. coli, t ...	1989	2546929
specificity of transposition of tn7 in vibrio parahaemolyticus.	tn7 was found to transpose at a high frequency from the plasmid, rp4::tn7, to the chromosome of vibrio parahaemolyticus. seven isolates carrying tn7 insertions were derived from three wild-type strains isolated from geographically distinct areas, and hindiii and bsteii dna digests of these strains were probed with a cole1::tn7 biotinylated probe. the results indicated that v. parahaemolyticus is similar to several other species which have been studied in having a highly preferred site of inserti ...	1989	2550986
mechanism of lhrh-stimulated steroidogenesis in rat leydig cells: lipoxygenase products of arachidonic acid may not be involved.	luteinizing hormone releasing hormone agonist, [(imbzl)-dhis6,pro9,net]-lhrh (lhrh-a), caused a two to threefold increase in in vitro testosterone (t) secretion by rat leydig cells. this lhrh-a-induced t secretion was completely blocked by quinacrine and chloroquine, inhibitors of phospholipase a2. addition of phospholipase a2, however, was ineffective in stimulating basal or lhrh-a-induced t secretion. phospholipase c, on the other hand, significantly stimulated both basal and lhrh-a-induced t ...	1989	2695506
[specificity of action of metalloproteinases from buckwheat seeds. hydrolysis of insulin beta-chain].		1989	2698338
effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets.	the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ...	1989	2556355
adherence targets of vibrio parahaemolyticus in human small intestines.	formalin-fixed human small intestinal mucosa with mucus coating, villi, and lymphoid follicle epithelium at the mucosal surface was used to test the adherence sites of clinically isolated (kanagawa phenomenon-positive) strains of vibrio parahaemolyticus. v. parahaemolyticus strains grown on cfa agar (supplemented with 3% nacl) for ca. 3 h at 37 degrees c possessed various levels of cell-associated hemagglutinins (has) which were detected with human or guinea pig erythrocytes. the observed adhere ...	1989	2568344
[acute gastroenteritis due to vibrio parahaemolyticus in spain. presentation of 8 cases].	eight cases of acute gastroenteritis caused by vibrio parahaemolyticus in humans are described; to our knowledge, they are the first such reported cases in spain. all cases appeared between august 20th and october 15th, with a frequency of 8.3% regarding the overall adult patients with acute gastroenteritis, and 11.5% regarding the overall patients with positive stool culture for any enteropathogenic organism. the eight strains were kanagawa positive and in three patients other enteropathogenic ...	1989	2716372
[acute gastroenteritis due to vibrio parahaemolyticus. serotypes and presentation of 4 new cases].		1989	2716381
purification of a tdh-related hemolysin produced by a kanagawa phenomenon-negative clinical isolate of vibrio parahaemolyticus 06: k46.	a hemolysin produced by a clinical isolate of kanagawa phenomenon-negative vibrio parahaemolyticus 06: k46 was purified by 55% ammonium sulfate fractionation and successive column chromatographies on deae-cellulose, hydroxyapatite, sepharose 4b and mono q. the purified hemolysin was physicochemically and immunologically identical with the vp-trh (v. parahaemolyticus thermostable direct hemolysin related hemolysin) recently described in v. parahaemolyticus 03: k6 (honda et al. infect. immun. 56: ...	1989	2721917
vibrio parahaemolyticus and other halophilic vibrios associated with seafood in hong kong.	the summer prevalence of vibrio parahaemolyticus and other halophilic vibrios in seafood from hong kong markets was investigated. halophilic vibrios were isolated from all seven types of seafood examined, and comprised 9.1%, 8% and 6.1% of contaminating aerobic heterotrophic bacteria from mussels, clams and oysters respectively. sucrose-positive vibrios were more common than sucrose-negative varieties. vibrio alginolyticus was the most frequently isolated species, followed by v. parahaemolyticus ...	1989	2722715
the cardiovascular effect of intracerebroventricular endothelin in rats.	to investigate the cardiovascular action of endothelin within the central nervous system, we studied the effect of intracerebroventricular endothelin in conscious wistar rats. the endothelin increased blood pressure and the heart rate in a dose-related way. the increase in mean blood pressure produced by 100 ng/kg of endothelin (45 +/- 6 mmhg, mean +/- s.e.m., n = 10) was much greater than that produced by the same amount of intravenous endothelin (4 +/- 1 mmhg, n = 7). pretreatment with intrave ...	1989	2576664
primary structures of sm31/32 diagnostic proteins of schistosoma mansoni and their identification as proteases.	we have constructed cdna clones containing the complete nucleotide sequences coding for two highly antigenic schistosoma mansoni adult worm proteins, sm31 and sm32. the predicted amino acid sequence of sm31 shows significant homology to mouse, rat and human cathepsin b. the nucleotide sequence of sm32 is identical to that reported by others for s. mansoni "haemoglobinase'. the different nucleotide sequences demonstrate the existence of two different proteolytic enzymes, both of which are synthes ...	1989	2725581
in vivo dna cross-linking by cyclophosphamide: comparison of human chronic lymphatic leukemia cells with mouse l1210 leukemia and normal bone marrow cells.	alkaline elution was done on a variety of cells following cyclophosphamide (cy) treatment in vivo. cells used were l1210 leukemia, normal mouse bone marrow, and peripheral blood cells obtained from a patient with chronic lymphatic leukemia (cll). endpoints used were determination of single strand breaks, dna-dna interstrand and dna-protein cross-links. after treatment of mice with cy (4 mg/mouse), low levels of single strand breaks were observed in both l1210 leukemia and cdf1 normal bone marrow ...	1989	2731167
ventricular arrhythmias and local electrograms after chronic regional denervation of the ischemic area in the pig heart.	cardiac denervation has been proved to reduce the incidence of coronary occlusion arrhythmias in digs, but the effect of limiting the extent of sympathectomy to the ischemic area, particularly in hearts with sparse coronary collateral circulation, as in the human heart, needs further investigation. ventricular arrhythmias and changes in epicardial direct current electrograms induced during acute left anterior descending coronary artery occlusion were recorded in 14 pigs subjected to regional den ...	1989	2738265
effect of urapidil on the performance of ischaemic myocardium in anaesthetized dogs.	the effect of urapidil on the ischaemic myocardium was studied in eight anaesthetized dogs. stenosis of the left descending coronary artery reduced blood flow and systolic contraction of the post-stenotic myocardium by about 50%; the end-diastolic length of the post-stenotic myocardium and the end-diastolic pressure increased, while aortic pressure slightly decreased. subsequent administration of urapidil (0.25 + 0.25 + 0.5 + 1.0 mg/kg intravenously) did not affect the systolic shortening and en ...	1989	2576670
[phylogenetic analysis of genes of the influenza virus. relationship between adaptability and neutrality].	detailed phylogenetic analysis of the gene family of hemagglutinin h3 of influenza a-type virus was fulfilled, taking into account the domain structure of protein and positions of antigen determinants. the densities of distribution of fixed synonimic replacements between domains ha1 and ha2 were shown to be actually equal (rho (ha1) = rho (ha2], and those of nonsynonimic ones to be unequal: their ratios were rho (ha1): rho (ha2) = 2.8 for nonepidemic branches, and rho (ha1): rho (ha2) = 7.7 for ...	1989	2583491
comparative amino acid sequence analysis of hemolysins produced by vibrio hollisae and vibrio parahaemolyticus.	vibrio hollisae produces a hemolysin (vh-rtdh) that is related to the thermostable direct hemolysin of vibrio parahaemolyticus (vp-tdh). although both hemolysins are essentially similar biologically and immunologically, they differ markedly in heat stability; vp-tdh is heat stable, whereas vh-rtdh is heat labile. to elucidate the relationships between their characteristics and molecular structures, we analyzed the amino acid sequence of vh-rtdh and compared it with that of vp-tdh. vh-rtdh consis ...	1989	2592352
cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of vibrio parahaemolyticus.	vibrio parahaemolyticus isolates derived from an outbreak of gastroenteritis in the republic of maldives did not have the genetic potential to produce the thermostable direct hemolysin, but one such isolate produced a hemolysin immunologically related to the thermostable direct hemolysin (t. honda, y. ni, and t. miwatani, infect. immun. 56:61-965, 1988). the maldives isolates hybridized with the dna probe for the gene encoding the thermostable direct hemolysin (the tdh gene) under reduced string ...	1989	2759706
emergence of a restricted bioserovar of vibrio parahaemolyticus as the predominant cause of vibrio-associated gastroenteritis on the west coast of the united states and mexico.	examination of 45 human fecal isolates of vibrio parahaemolyticus revealed the emergence of an unusual bioserovar (o4:k12, urease positive) associated with cases of gastroenteritis which appear to be domestically acquired on the west coast of the united states and mexico.	1989	2592553
[prospects for measuring the rheologic characteristics of human soft tissues based on the recording of their transverse rigidity].	it is shown by an example of a forearm that to determine objective characteristics of rheological properties of soft human tissues by measuring their transverse stiffness (k) one should first investigate the relation between k and the diameter of the used indentor (d). the nonlinearity of the dependence k(d) for indentor diameters from 2 to 8 mm is demonstrated and it is assumed that this nonlinearity is due to heterogeneity of tissue properties in the directions normal to the axis of force acti ...	1989	2765581
a mutant hemolysin with lower biological activity produced by a mutant vibrio parahaemolyticus.	a mutant toxin (m-tdh) of thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus was isolated from the culture of a strain of this organism mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. although the m-tdh had a molecular structure similar to the native vp-tdh, the m-tdh retained only about 7% residual hemolytic activity of the native toxin. furthermore, other biological activities of m-tdh, such as lethality in mice and enterotoxicity in rabbit ileal loops, were also weakened ...	1989	2599363
transcriptional start site in the mouse cyp1a1 (cytochrome p1450) gene.	two transcriptional initiation sites have been described by different laboratories for the mouse cyp1a1 (cytochrome p1450) gene: start site ti, at the beginning of the nontranslated 87-bp first exon, and start site tii, 635 bp downstream from site ti within the 2,380-bp first intron. site ti was characterized in normal c57bl/6 mouse liver, whereas site tii was described in a high activity variant line (hav cells) derived from the mouse hepatoma hepa-1c1c7 wild type (wt) established cell line. it ...	1989	2766934
prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6.	the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse.	1989	2613230
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.	we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...	1989	2769548
studies in fetal wound healing: ii. a fetal environment accelerates fibroblast migration in vitro.	we have used an in vitro model of wound healing using scratches made in a confluent monolayer of fibroblasts. the effects of fetal calf and postnatal calf serum on the migration of fibroblasts were compared. differences between fetal and calf serum-incubated fibroblasts grown on coverslips were observed within 15 minutes of exposure. cells in fetal serum began to change both shape and orientation and to move into the trough created by the scratch. the fibroblasts incubated in fetal calf serum co ...	1989	2769548
gene mapping by enzymatic amplification from flow-sorted chromosomes.	a new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. reliable amplification from as few as 200 chromosomes has been demonstrated. this method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, thy-1, and t3 delta and distal to the int-2 gene. t ...	1989	2613232
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
characterization of a non-structural 147-kda precursor polypeptide of echovirus 33 and its immunogenicity in man.	zinc chloride (0.9 mm)--an inhibitor of the processing of the initial polypeptides of picornaviridae--was used to accumulate large precursors of echovirus 33 (ev33) and notably two proteins of 147- and 97-kda. polyclonal hyperimmune sera were raised in mice against these polypeptides and assayed by immunoblotting against ev33-infected cells blocked or not with zncl2, showing that protein 147--2abc3abcd according to the l434 convention--can be considered to be the precursor of the two non-structu ...	1989	2772414
heterosexual spread of hiv infection.		1989	2772471
haemolysins of vibrio parahaemolyticus strains passaged in mice.	some phenotypically non-haemolytic strains of v. parahaemolyticus were observed to produce haemolysins after two passages in mices. depending on the strain, both heat stable and heat labile haemolysins were induced. the heat stable haemolysin so induced, was immunologically different from the thermostable direct haemolysin produced by kanagawa positive strains of v. parahaemolyticus.	1989	2620943
primary structure of a novel 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits)-binding membrane protein highly expressed in torpedo californica electroplax.	polyclonal rabbit antibodies were raised against 4-acetamido-4'-isothiocyanostilbene-2,2'-disulphonic acid (sits), an inhibitor of a variety of anion transport proteins. these antibodies specifically recognize sits-reacted erythrocyte band 3 in immunoprecipitations and western blots. in western blots of sits-reacted membrane proteins derived from vesicles of the electric organ of torpedo californica (known to express a sits-sensitive cl- channel) the antibodies recognized two major species of ap ...	1989	2775201
[study on 438 strains of pathogenic vibrio and their resistance to drugs].	this paper reported the monitoring result of pathogenic vibrio from 1461 diarrhoea outpatients in haikou city from april to november 1986. 438 strains of pathogenic vibri (29.98%) had been identified. among them the vibrio parahemolyticus (14.99%) was more common and the next was non-0:1 v. cholerae strains (11.84%). the features of attack-time, age, sex and clinical symptoms etc. were discussed.	1989	2624932
effects of food preservatives and local anesthetics on synthesis of outer membrane proteins in vibrio parahaemolyticus.	the effects of seven food preservatives including salicylate, benzoate, dehydroacetate, sorbate and propionate, and two local anesthetics, phenethyl alcohol and procaine, on the synthesis of major outer membrane proteins in vibrio parahaemolyticus strain 3283-61 were investigated. the synthesis of the outer membrane proteins a, b, c and e was remarkably inhibited by all the food preservatives except propionate and two additional outer membrane proteins with respective molecular weights of about ...	1989	2626751
formation of hydroxynorspermidine from exogenously added 1,3-diaminopropan-2-ol in vibrio species.	when vibrio parahaemolyticus aq 3627 was grown in the presence of 1,3-diaminopropan-2-ol (oh-dap), a new compound accumulated in the cells. this was identified as hydroxynorspermidine (oh-nspd), n-(3-aminopropyl)-1,3-diaminopropan-2-ol, by gas chromatography-mass spectrometry and thin-layer chromatography. it was also synthesized enzymatically from oh-dap by a cell-free preparation from this strain. all other vibrio strains examined also showed the ability to synthesize this compound, but none o ...	1989	2632061
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
[the sympathetic and renin-angiotensin systems are complementary in blood pressure regulation].	the consequences of a chronic destruction of sympathetic nerves or of the blockade of the renin angiotensin system (ras) on the blood pressure (bp) level and its spontaneous variability were studied using a computerized method which allows a continuous monitoring of bp in conscious unrestrained rats. guanethidine, used to lesion the sympathetic fibres did not alter the bp level but significantly enhanced its variability. on the opposite, angiotensin converting enzyme inhibition decreased the bp ...	1989	2633862
in vitro cultures of trabecular meshwork cells of the human eye as a model system for the study of cellular aging.	cells from the human trabecular meshwork providing a drainage system for the outflow of aqueous humour in the eye were isolated and propagated in monolayer culture. following serial subcultivation of the primary cultures, there was a gradual decline in the fraction of dividing cells with increasing population doubling level (pdl) resulting finally in growth cessation and disintegration of these 'senescent' cultures. the number of population doublings was at most 20. senescent cultures revealed r ...	1989	2640083
human t lymphocytes possess a limited in vitro life span.	the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ...	1989	2786475
[effect of two stage corticotomy on maxillary protraction].	for the purpose of clarifying the effects of two stage corticotomy on maxillary protraction, experimental studies by using of 5 male japanese monkeys (macaca fuscata) were performed. results from cast analysis, roentgenographic cephalometry and histological examinations were as follows. i. in experimental groups that received two stage corticotomy with maxillary protraction, apical base of maxillary bone protruded markedly. ii. in control groups that maxillary protraction was applied without cor ...	1989	2640929
synergistic antitumor effects of interleukin 2 and the monoclonal lym-1 against human burkitt lymphoma cells in vitro and in vivo.	interleukin 2 (il-2) regulates immune responses by inducing proliferation and differentiation of t-cells into cytotoxic cells, inducing lymphokine activated killer activity and enhancing antibody dependent cellular cytotoxicity (adcc). lym-1, a monoclonal antibody, recognizes a membrane antigen present on the surface of b-lymphoma cells and can be used for adcc. we therefore used raji (human burkitt lymphoma) cells to study the efficacy of combination therapy with il-2, lymphokine activated kill ...	1989	2788499
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.	previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...	1989	2789310
[an autopsy case of vibrio parahaemolyticus sepsis].		1989	2794678
ketopantoyl-lactone reductase from candida parapsilosis: purification and characterization as a conjugated polyketone reductase.	ketopantoyl-lactone reductase (2-dehydropantoyl-lactone reductase, ec 1.1.1.168) was purified and crystallized from cells of candida parapsilosis ifo 0708. the enzyme was found to be homogeneous on ultracentrifugation, high-performance gel-permeation liquid chromatography and sds-polyacrylamide gel electrophoresis. the relative molecular mass of the native and sds-treated enzyme is approximately 40,000. the isoelectric point of the enzyme is 6.3. the enzyme was found to catalyze specifically the ...	1989	2644973
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro].		1989	2806660
dexamethasone regulation of the expression of cytokine mrnas induced by interleukin-1 in the astrocytoma cell line u373mg.	bsf-2/il-6, gm-csf and il-1 beta mrnas were induced by recombinant il-1 in human astrocytoma cell line u373mg. the induction of bsf-2/il-6 and il-1 beta mrnas did not require de novo protein synthesis while that of gm-csf mrna required a newly synthesized protein. dexamethasone inhibited the induction of these cytokine mrnas by il-1. this process seems to require continued protein synthesis. these results suggest that the production of these cytokines are positively and negatively controlled by ...	1989	2646146
a role for hemispheric asymmetry in human behavioral variability.	a model is presented in which functional lateral specialization and selective hemisphere priming combine to provide a mechanism for behavioral variability. the model is tested and supported by two experiments in which lateral attentional priming produces significant reductions in between-subject and within-subject variability. these findings help us to understand the source of behavioral variability, broaden our knowledge of the gross structures and functions of the brain, provide information ab ...	1989	2657608
mechanics of the healed meniscus in a canine model.	the mechanical behavior of the intact canine stifle joint was studied, using a surgical model of meniscal injury and repair. thirty-eight animals were divided into five study groups: group s received only a sham arthrotomy (without a meniscal incision), groups p13 and p26 received peripheral medial meniscal incisions, and groups r13 and r26 received radial medial meniscus incisions. all meniscal incisions were repaired anatomically with absorbable suture. groups p13 and r13 were sacrificed at 13 ...	1989	2667375
[biological characteristics of plasmid carrying a repeated deoxyribonucleic acid sequence].	it was found that a plasmid which had a foreign deoxyribonucleic acid (dna) between two repeated sequences did not multiply in e. coli recbcsbcb, even if it multiplied in wild-type e. coli, e. coli recbc or e. coli recbcsbcbrecf when the insert was longer than 351 base pair. the multiplication of these plasmids were, however, inhibited when a plasmid expressing recf gene was introduced into e. coli recbcsbcbrecf. the inviability of the plasmid carrying the repeated sequence in e. coli recbcsbcb ...	1989	2681680
characterization of toxigenic vibrios isolated from the freshwater environment of hiroshima, japan.	the occurrence and characterization of toxigenic vibrios in surface water and sediment samples of the fresh water environment of the ohta river were studied. the membrane filter, pad preenrichment technique, followed by the placement of membranes onto thiosulfate citrate-bile salt-sucrose agar, was used for the enumeration of total vibrios. qualitative examination of pathogenic vibrios was also attempted. in addition, a survey was conducted to determine the incidence of clostridium botulinum in ...	1989	2690736
[transurethral unroofing of the müllerian duct cyst: a case report].	management of müllerian duct cyst depends on the size and symptomatology. here we report a case of recurrent müllerian duct cyst which was treated successfully by transurethral approach. a 57-year-old man was first admitted because of dysuria in 1981. under the diagnosis of müllerian duct cyst, he underwent resection of the cyst by suprapubic retrovesical approach that resulted in incomplete extirpation. in 1982, he was readmitted for recurrence of the cyst. excision by transsacral approach was ...	1989	2694830
[bacterial etiologies of acute infantile diarrhea in dakar].	systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica.	1989	2491376
immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen.	we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ...	1989	2491623
lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats.		1989	2492809
in vitro development of neospora caninum (protozoa: apicomplexa) from dogs.	the development of neospora caninum isolated from naturally infected dogs was examined in mammalian cell cultures. tachyzoites developed by endodyogeny when inoculated onto bovine monocyte or bovine cardiopulmonary artery endothelial cell cultures. tachyzoites were 5.0 by 2.0 microns and had a posteriorly located nucleus. cytopathogenic effects of parasite development consisted of the formation of holes in the cell monolayer associated with the rupture of infected host cells. serial passage of t ...	1989	2493088
symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984.	the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ...	1989	2609210
[morphometric and histologic studies of the significance of the paraventricular nucleus for the thyroid gland axis after ganglionectomy and cold exposure with special consideration of the pineal gland].	to analyse the role of the peripheral sympathetic fibres for the nucleus paraventricularis, the nuclear size and the neurosecretory material were studied 30 d after bilateral cervical ganglionectomy (gx). in order to examine the influence of gx at normal temperature (24 degrees c) and exposure to cold (10 degrees c), rats were subjected to a 72 h exposure to cold before killing. additionally thyroxine (t4), serum thyrotropin (tsh), and pituitary tsh were investigated. 4 times a day (light-dark c ...	1989	2610374
three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout.	the nerve endings of normal hair of the rat's snout, partially digested with trypsin and hydrochloric acid, were studied by scanning electron microscopy. each lanceolate structure measured ca. 10 microns in length and was arranged around the hair follicle. these palisade-shaped nerve endings were situated almost beneath the sebaceous glands, ran upward, parallel to the axis of the hair follicle, and terminated in pointed shape. 2 kinds of cells, teloglia cell type i showing flat profile, and tel ...	1989	2610376
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.	we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...	1989	2612450
effects of postnatal dexamethasone treatment on development of alveoli in adult rats.	we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ...	1989	2612450
human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin.	human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ...	1989	2612455
human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin.	human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ...	1989	2612455
[hiv infection and aids in 1988. the epidemiological situation in norway].		1989	2705132
new techniques of analyzing the healing process of artificial vascular grafts, transmural vascularization, and endothelialization.	beside traditional techniques (light, immunofluorescence, transmission electron (tem)- and scanning electron microscopy (sem), a new more sensitive method for producing microcorrosion casts using a polyester-based resin has been developed. the sem analyses of the microcorrosion casts of alloplastic vascular grafts was realized in an absolutely stable stage of polymerization. for the first time, a transprosthetic vascularization could be shown in great three-dimensional detail. the importance of ...	1989	2711038
role of extracellular space in hyperosmotic suppression of potassium-induced electrographic seizures.	1. focal electrographic seizures arose in the ca1 region of rat hippocampal slices bathed in elevated (8.5 mm) external potassium [( k+]o). high [k+]o also induced spontaneous interictal bursts that originated in area ca3 and propagated to ca1. to examine the contribution to electrographic seizure initiation of excitatory mechanisms that are influenced by extracellular volume, we studied the effect of hyperosmotic expansion of interstitial volume on seizure occurrence, interictal bursts, and exc ...	1989	2723735
bronchoconstrictor action of neuropeptide y (npy) in isolated guinea pig airways.	responses of various preparations of guinea pig and rat airways to synthetic porcine neuropeptide y were analysed in vitro. npy in doses up to 10(-6) m, induced a dose dependent contraction in trachea, bronchi and lung parenchymal strips of the guinea pig but did not have any effect in similar preparations obtained from the rat. in guinea pig airways the contractile responses to npy were small in size and characterized by a slow onset and a long duration. this effect of the peptide was not depen ...	1989	2747854
production and characterization of a compound inhibitory to vibrio parahaemolyticus from proteus vulgaris.	the production and characteristics of a compound in proteus vulgaris g cultures which was capable of inhibiting vibrio parahaemolyticus and other food-borne pathogens was investigated. production was influenced by medium composition, ph and temperature but not by the extent of aeration. the compound was most inhibitory at the optimum temperature for growth of v. parahaemolyticus. the inhibitor was most stable at ph 7.0 and inhibition occurred even after heating at 70 degrees c for 30 min and aft ...	1989	2613590
in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea.	the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ...	1989	2613604
standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera.	a non-competitive enzyme-linked immunoassay (elisa) has been standardized to supplement the in vivo potency test used for the quality control of inactivated tissue culture vaccines against rabies. the essentials of the elisa were: fixation of the virus in different dilutions of vaccine on the surface of microtitre plates; testing of the reference and up to six test vaccines on one plate; incubation with polyclonal antisera to rabies virus glycoprotein containing an excess of antibody; further in ...	1989	2613705
[the detection of vibrio parahaemolyticus and vibrio alginolyticus using modified culture media].	studies have been completed for the detection of vibrio(v.) parahaemolyticus and v. alginolyticus in baltic sea fish. the author had prepared for that purpose a liquid culturing medium and a solid substrate. the percentage of positive findings is remarkable. the results so far recorded should be a good reason for further studies.	1989	2619483
3'-fluoro-2',3'-dideoxy-5-chlorouridine: most selective anti-hiv-1 agent among a series of new 2'- and 3'-fluorinated 2',3'-dideoxynucleoside analogues.	a series of 2'- and 3'-fluorinated 2',3'-dideoxynucleosides and 3'-azido-2',3'-dideoxynucleosides were synthesized and evaluated for their inhibitory activity against human immunodeficiency virus-1 (hiv-1) replication in mt-4 cells. neither conversion of 3'-fluoro- or 3'-azido-2',3'-dideoxyadenosine to the corresponding inosine derivatives nor 8-bromination of 2',3'-dideoxyadenosine resulted in increased anti-hiv-1 activity. nor did introduction of a 2'-fluorine in the erythro or threo configura ...	1989	2754700
isolation of the saprophytic strain of mc-3 and participation of the cell surface structure in predation.	from a predatory bacterium, mc-3, a mutant strain which lost predation ability was isolated by chance selection. biological properties of the mutant were the same as the parent except only saprophytic property. properties of the parent and the mutant strains of mc-3, such as bacteriolytic activity of the culture supernatant, digestion of peptidoglycan of the host bacteria, and growth by utilizing the host cells or their cytoplasmic substances, suggested that cell surface structure of the host ce ...	1989	2622396
identification and characterization of an 8-kd peptide stimulating late erythropoiesis.	chronic renal failure is often associated with severe anemia, subnormal levels of erythropoietin (epo), and the presence of erythropoietic inhibitors. we studied an anephric patient with polycystic kidney disease maintaining a hemoglobin level of 13-14 g. biochemical, endocrine, and hematological parameters were all within normal limits. epo levels examined on two occasions during the clinical investigation were found to be low to subnormal. plasma of this patient supported human bone marrow ery ...	1989	2753086
[complete occlusion both left main coronary artery and right coronary artery].	a 67-year-old man, who had complete occlusion both of left main coronary artery (lmca) and right coronary artery (rca), underwent coronary revascularization. he had been suffering from severe angina pectoris preoperatively. exercise electrocardiogram (single master) showed myocardial ischemia. on cardiac catheterization, he showed extensive collaterals from right main coronary artery, and well-preserved left ventricular function. at operation, coronary arteries revealed severe sclerosis, especia ...	1989	2770071
[prevalence of vibrio parahaemolyticus in seafood from restaurants in the city of mérida, yucatán].	we report the prevalence of the vibrio parahaemolyticus in raw (11.54%), insufficiently cooked (0.00%) and partially cooked with heat (4.55%) sea foods, from restaurants of the city of merida, yucatan, mexico. we studied 190 samples from march to august 1987. we isolated 12 strains (6.32%) from an equal number of samples, whose biochemical characteristics corresponded with vibrio parahaemolyticus. we didn't find significant statistically differences when we compared the prevalences of the sample ...	1989	2772734
endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium.	the inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. endothelium-dependent responses were examined in vitro in normal yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (lad) 4 weeks before the study (n = 10). the presence of a complete endothelial lining was confirmed histologic ...	1989	2766515
compartmentalizing chemomechanical transduction in the ejecting heart.	the efficiency of transferring the total energy generated by ventricular contraction (pressure-volume area, pva) to external work (ew) and internal work (iw) and the myocardial oxygen consumption (mvo2) at zero pva were determined during volume loading on right heart bypass before and after a 50% augmentation (cacl2, 0.03 meq/kg/min, n = 7) or depression (20 minutes of 37 degrees c ischemia with 30 minutes of reperfusion, n = 7) of the contractile state. an increased ew efficiency (64% +/- 7% vs ...	1989	2763028
hypoxic changes in hippocampal neurons.	1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ...	1989	2547034
[acute maternal anterior poliomyelitis in a non-endemic zone].	the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus.	1989	2561040
concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated.	because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ...	1989	2561041
in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues.	one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ...	1989	2565962
effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes.	electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ...	1989	2566207
genome type analysis of adenoviruses: isolates from one year from the hannover area.	adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ...	1989	2566302
time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated cycles.	in this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ...	1989	2515208
the estimation of dietary protein intake in chronic renal failure.	forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ...	1989	2515492
use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5.	insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ...	1989	2538799
advances in oculoplastic surgery.	ophthalmic plastic surgery, a sub-specialty within ophthalmology, encompasses diagnosis and treatment of diseases and abnormalities of the eyelids, orbit, and lacrimal systems. eyelid carcinoma is a more common entity as well as blepharoptosis seen in children and adults. it can usually be corrected by surgery involving the levator palpebrae superioris or a tarsofrontalis suspension using synthetic material or human tissue. retraction of the upper eyelid, the opposite of blepharoptosis, may be s ...	1989	2592933
exotic diarrhoeal problems and poliomyelitis.	exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death.	1989	2594659
identification of n1-acetylnorspermidine in vibrio parahaemolyticus and an enzyme activity responsible for its formation.	n1-acetylnorspermidine [ch3conh(ch2)3 nh(ch2)3nh3] was identified in vibrio parahaemolyticus, which contains norspermidine as a major polyamine. this is the first example for the natural occurrence of monoacetylated unusual polyamine. the n1-acetylnorspermidine content was the highest 4 h after inoculation. incubation of norspermidine and acetyl coa with a cell extract from v. parahaemolyticus produced n1-acetylnorspermidine. a remarkable increase in specific activity of the acetyltransferase wa ...	1989	2599358
evidence of immunologic cross-reactivity between hemolysins of vibrio hollisae and vibrio parahaemolyticus demonstrated by monoclonal antibodies.		1989	2584761
urease-positive, kanagawa-negative vibrio parahaemolyticus from patients and the environment in the pacific northwest.	we previously reported the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. the present studies compare the biochemical characteristics, kanagawa hemolysin reactions, and plasmid profiles of 13 patient and 221 environmental isolates of the organism. classical biochemical testing of the isolates revealed similar reactions for the clinical and environmental strains, and analysis in agarose gels revealed that 13 to 15% of the isolates had plasmids. the ...	1989	2592543
enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus.	we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ...	1989	2556341