Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cecal and fecal bacterial flora of the mongolian gerbil and the chinchilla. | the mongolian gerbil is being increasingly used as a laboratory animal and as a pet. both chinchillas and gerbils are used as animal models for otitis media and other otic research. previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. using the strict anaerobic methodology of the virginia polytechnic institute anaerobe laboratory, we studied the predominant bacterial flora of the cecum and feca ... | 1988 | 3389814 |
| cefetamet pivoxil: bacteriostatic and bactericidal activity of the free acid against 355 gram-negative rods. | the in vitro activity of the free acid of cefetamet pivoxil (ro 15-8075) was tested against 355 clinical isolates, namely enteropathogenic bacteria, glucose non-fermentative gram-negative rods (excluding pseudomonas aeruginosa) and legionella pneumophila. ceftriaxone was included in the study as reference compound. although the free acid of the orally active cephalosporin was generally weaker than ceftriaxone, it inhibited 88.2% and 94.5% of enterobacteriaceae and vibrionaceae at a concentration ... | 1988 | 3403040 |
| partial nucleotide sequence of the chlorocatechol degradative operon tfdcdef of pjp4 and similarity to promoters of the chlorinated aromatic degradative operons tfda and clcabd. | 1988 | 3405772 | |
| inducible and constitutive expression of pmol28-encoded nickel resistance in alcaligenes eutrophus n9a. | the nickel and cobalt resistance plasmid pmol28 was transferred by conjugation from its natural host alcaligenes eutrophus ch34 to the susceptible a. eutrophus n9a. strain n9a and its pmol28-containing transconjugant m220 were studied in detail. at a concentration of 3.0 mm nicl2, the wild-type n9a did not grow, while m220 started to grow at its maximum exponential growth rate after a lag of 12 to 24 h. when grown in the presence of subinhibitory concentrations (0.5 mm) of nickel salt, m220 grew ... | 1988 | 3410828 |
| cloning of the alcaligenes eutrophus alcohol dehydrogenase gene. | mutants of alcaligenes eutrophus which are altered with respect to the utilization of 2,3-butanediol and acetoin were isolated after transposon mutagenesis. the suicide vehicle psup5011 was used to introduce the drug resistance transposable element tn5 into a. eutrophus. kanamycin-resistant transconjugants of the 2,3-butanediol-utilizing parent strains cf10141 and as141 were screened for mutants impaired in the utilization of 2,3-butanediol or acetoin. eleven mutants were negative for 2,3-butane ... | 1988 | 2828319 |
| three-dimensional model of stellacyanin and its implications for electron transfer reactivity. | experimental data were combined with computational methods in constructing a hypothetical three-dimensional model for the blue single copper protein rhus stellacyanin (st). the known sequence of stellacyanin and its homology with plastocyanin (pc) were used together with the results of spectroscopic studies of the protein that yielded the current assignment of two histidines, one cysteine and a disulfide sulfur as copper ligands in stellacyanin. by computer graphics and energy minimization the f ... | 1988 | 2975707 |
| in vitro susceptibility of alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents. | the in vitro susceptibilities of 37 clinical isolates of alcaligenes denitrificans subsp. xylosoxidans to 24 antimicrobial agents were determined. imipenem was the only drug with consistent activity (mic for 90% of isolates, 2 micrograms/ml). piperacillin, ticarcillin-clavulanic acid, ceftazidime, and co-trimoxazole were active against most strains. all the isolates were resistant to ampicillin, cefazolin, cefuroxime, cefamandole, cefotetan, ceftriaxone, cefotaxime, aztreonam, amdinocillin, and ... | 1988 | 3163242 |
| regulation of isofunctional enzymes in pseudomonas alcaligenes mutants defective in the gentisate pathway. | the regulation of the inducible set of gentisate pathway enzymes used by pseudomonas alcaligenes (p25x1) has been studied in strains derived from mutant strains of p25x1 that had lost the constitutive enzymes that degrade m-cresol, 2,5-xylenol and 3,5-xylenol. the enzyme, 3-hydroxybenzoate 6-hydroxylase ii, that catalyzes the oxidation of 3-hydroxybenzoate to gentisate is substrate- and product-induced while gentisate dioxygenase ii is substrate induced. neither 3-hydroxybenzoate nor gentisate c ... | 1988 | 3170385 |
| resonance raman spectra of the copper-sulfur chromophores in achromobacter cycloclastes nitrite reductase. | resonance raman spectroscopy at ambient temperature and 77 k has been used to probe the structures of the copper sites in achromobacter cycloclastes nitrite reductase. this enzyme contains three copper ions per protein molecule and has two principal electronic absorption bands with lambda max values of 458 and 585 nm. comparisons between the resonance raman spectra of nitrite reductase and blue copper proteins establish that both the 458 and 585 nm bands are associated with cu(ii)-s(cys) chromop ... | 1988 | 3170560 |
| alcaligenes piechaudii from chronic ear discharge. | a recently described bacterium, alcaligenes piechaudii, was isolated repeatedly from the ear discharge of a diabetic man. this appears to be the first report of human infection in which there is clinical evidence of a pathogenic role for this species. | 1988 | 3170714 |
| nosocomial outbreak of achromobacter xylosoxidans associated with a diagnostic contrast solution. | 1988 | 3170779 | |
| chlorobenzoate catabolism and interactions between alcaligenes and pseudomonas species from bloody run creek. | a mixed community of bacteria from surface runoff waters of the hyde park industrial landfill was enriched on 3-chlorobenzoate. alcaligenes and pseudomonas species were dominant in the community. alcaligenes sp. br60 carried an unstable plasmid specifying 3-chlorobenzoate catabolism. metabolites detected in culture supernatants included chlorocatechol and chloro-cis, cismuconic acid. oxygen uptake in the presence of 3- and 4-substituted methyl-catechols revealed a catechol-1,2-oxygenase activity ... | 1988 | 3178396 |
| gene duplication in haloaromatic degradative plasmids pjp4 and pjp2. | pjp2 and pjp4 are 2,4-dichlorophenoxyacetic acid catabolic plasmids, and they show dna sequence homology. most of the pjp2 molecules (80% or more) isolated from 2,4-dichlorophenoxyacetic acid grown cells of alcaligenes eutrophus harbor a tandem duplication of a 25-kilobase (kb) segment encoding the catabolic functions. unlike plasmid pjp4, pjp2 in a. eutrophus gives rise to a 3-chlorobenzoate phenotype without further genetic rearrangement. pjp4 under 3-chlorobenzoate selection contains an inver ... | 1988 | 3203254 |
| structure of azurin from alcaligenes denitrificans refinement at 1.8 a resolution and comparison of the two crystallographically independent molecules. | the structure of the blue copper protein azurin, from alcaligenes denitrificans, has been refined crystallographically by restrained least-squares methods. the final crystallographic r value for 21,980 observed reflections to 1.8 a (1 a = 0.1 nm) resolution is 0.157. the asymmetric unit of the crystal contains two independent azurin molecules, the model for which comprises 1973 protein atoms, together with three so2-4 ions, and 281 water molecules. comparison of the two molecules shows very high ... | 1988 | 3210236 |
| [utilization of 3-chlorobenzoic acid by a mixed culture of microorganisms]. | a mixed microbial culture (acinetobacter calcoaceticus inmi-kz-3 and alcaligenes faecalis inmi-kz-5) completely utilises 3-chlorobenzoic acid (3-cba), and the liberation of chlorine atoms is 100% of the theoretically possible one. a. faecalis growth in the mixed culture at the account of 2-chloro-cis,cis-muconic acid which is accumulated in the course of 3-cba metabolism by a. calcoaceticus inmi-kz-3. | 1988 | 3211007 |
| growth dependent enzymatic profiles of some gram-negative nonfermentative bacteria of clinical significance. | a total of 734 strains of gram-negative nonfermentative bacteria (46 species and biochemically defined groups) of the genera pseudomonas, alcaligenes, bordetella, moraxella, acinetobacter, agrobacterium, and flavobacterium were investigated for their ability to hydrolyze 25 different chromogenic substrates. all tests were carried out in growth-stimulating media. results were read photometrically and evaluated automatically following a 24-h incubation. many of the 46 different species and biochem ... | 1988 | 3223128 |
| bacillus alcaligenes fecalis septicemia and meningitis in the newborn. | 1988 | 3225035 | |
| enzymatic process for the production of pharmacologically useful nucleosides. | 1988 | 3228238 | |
| [antibiotic sensitivity of nonfermenting gram-negative bacteria]. | antibiotic resistance of 132 strains of nonfermenting gram-negative bacteria (ngnb) was studied. 43, 20, 17, 14 and 12 of them belonged to acinetobacter calcoaceticus (anitratus and lwoffi), pseudomonas cepacia, alcaligenes faecalis, p. stutzeri and p. maltophilia, respectively. with rare exceptions all the strains were resistant to benzylpenicillin, oxacillin, lincomycin, ampicillin and cephaloridine. sensitivity to the other antibiotics varied within wide ranges. amikacin (94.3 per cent) and t ... | 1988 | 3228322 |
| bioavailability of zinc and cadmium and their effect on microbial growth and metal uptake. | 1988 | 3233390 | |
| [study of the microflora of fish and fish products on ships]. | 1988 | 3234820 | |
| achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound. | the microbiological and clinical features of a case of achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) meningitis associated with a gunshot wound are described. to our knowledge, this is the third confirmed case report of meningitis caused by this organism. | 1988 | 3235669 |
| [the role of gram-negative non-fermenting bacteria in hospital infections]. | 1988 | 3237527 | |
| two quick methods for isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase. | methods are described which allow the isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) in a very short time. source of the material was highly impure commercial enzyme in the case of spinach rubisco or bacteria grown from a fermentor in the case of alcaligenes eutrophus rubisco. purity of the enzymes is demonstrated by gel electrophoreses. enzyme isolated from fresh cells gave crystals of excellent diffraction, suitable for x-ray structure analyisis. | 1988 | 3237649 |
| [a clinical evaluation of sultamicillin fine granules in the treatment of meibomianitis]. | this study was performed to evaluate the clinical efficacy and the safety of sultamicillin (sbtpc) fine granules in the treatment of patients with meibomianitis. a dose of 375 mg sbtpc granules was orally given to 10 patients with meibomianitis after each meal, three times a day. the results obtained are summarized as follows. efficacies were rated as good in 7 cases, fair in 2 and poor in 1 with an efficacy ratio of 70.0%. no side effects were observed throughout the study. the organisms isolat ... | 1988 | 3249376 |
| bacterial species detected in some bottled mineral waters sold in italy. | a total of 51 samples from 32 different kinds of mineral water were examined to investigate their microbial facies in view of a possible relationship with ionic composition. no difference was found between mineral and oligomineral waters. the genera most represented were pseudomonas, flavobacterium and alcaligenes; only rarely could members of these genera be assigned precisely to a defined species, because many strains showed intermediate patterns. the authors suggest widening the microbiologic ... | 1988 | 3253227 |
| refinement of the structure of pseudoazurin from alcaligenes faecalis s-6 at 1.55 a resolution. | the crystal structure of the redox protein pseudoazurin (123 amino acid residues; molecular weight 13,000 daltons) from alcaligenes faecalis has been refined by fast fourier restrained least-squares minimization. cycles of rebuilding were carried out to escape from local minima. individual isotropic temperature factor values were refined separately for all atoms. the r factor was reduced from 0.400 (for 2647 reflections in the 6.0-2.8 a resolution range) to 0.180 (for all 19,770 reflections in t ... | 1988 | 3271558 |
| nosocomial achromobacter xylosoxidans infections. | a xylosoxidans is being recognized as an important nosocomial pathogen. as more patients are rendered immunosuppressed by chemotherapy, this organism's increasing role in hospital-acquired infections will be assured. achromobacter is a water-borne organism, highly resistant to most antibiotics, and even to some disinfectant solutions, and easily establishes itself in the hospital aquatic environment. achromobacter infections and outbreaks should be recognized and approached as serious problems r ... | 1988 | 3278052 |
| identification of pseudomonas, flavobacterium, and alcaligenes with the api 20 ne system. | the api 20 ne system is designed for the rapid identification of gram negative rods other than enterobacteriaceae. it has been compared to conventional methods in the characterization of 404 strains representative of 23 species belonging to 3 genera: pseudomonas (236 strains), flavobacterium (133 strains) and alcaligenes (35 strains). the system consists of 9 enzymatic tests and 12 carbohydrate assimilation tests. a 7-digit numerical profile is obtained by an octal coding of the test results. th ... | 1988 | 3279389 |
| achromobacter xylosoxidans (alcaligenes xylosoxidans subsp. xylosoxidans) bacteremia associated with a well-water source: case report and review of the literature. | a case of community-acquired achromobacter xylosoxidans bacteremia in a patient with metastatic breast carcinoma is described. the patient's home drinking water was identified as the source of her bacteremia. the case represents the first in which a community-acquired infection due to this organism has been attributed to a documented water source. | 1988 | 3281982 |
| 2-deoxy-2-fluoro-d-glycosyl fluorides. a new class of specific mechanism-based glycosidase inhibitors. | mechanism-based glycosidase inhibitors are of considerable use in studies of enzyme mechanism, in studies of glycoprotein processing, and possibly therapeutically in control of sugar uptake. this paper describes a new general approach to mechanism-based inactivation of glycosidases which involves trapping a covalent glycosyl enzyme intermediate. this is achieved by use of 2-deoxy-2-fluoro-d-glycosyl fluorides, for which the rate of hydrolysis of the fluoroglycosyl enzyme intermediate is extremel ... | 1988 | 3286645 |
| nickel affects expression of the nickel-containing hydrogenase of alcaligenes latus. | the effects of nickel on the expression of hydrogenase in the hydrogen-oxidizing bacterium alcaligenes latus were studied. in the absence of added nickel, both hydrogenase activity, measured as o2-dependent h2 uptake, and hydrogenase protein, measured in a western immunoblot, were very low compared with the levels in cells induced for hydrogenase in the presence of nickel. hydrogenase activity and protein levels were dependent on the added nickel concentration and were saturated at 30 nm added n ... | 1988 | 3045080 |
| isolation and characterization of a penicillinase from pseudomonas cepacia 249. | pseudomonas cepacia has an inducible beta-lactamase which is responsible for its novel ability to catabolize beta-lactam compounds. the gene encoding this enzyme, pena, was cloned from a genomic library of p. cepacia 249 on the broad-host-range cosmid plafr. this separated the pena gene from the gene encoding a second beta-lactamase in p. cepacia 249. expression of pena was inducible in an escherichia coli host strain by low levels of penicillin. the 33,500-molecular-weight enzyme had penicillin ... | 1988 | 3046483 |
| cloning and expression in escherichia coli of the alcaligenes eutrophus h16 poly-beta-hydroxybutyrate biosynthetic pathway. | the poly-beta-hydroxybutyrate (phb) biosynthetic pathway from alcaligenes eutrophus h16 has been cloned and expressed in escherichia coli. initially, an a. eutrophus h16 genomic library was constructed by using cosmid pvk102, and cosmid clones that encoded the phb biosynthetic pathway were sought by assaying for the first enzyme of the pathway, beta-ketothiolase. six enzyme-positive clones were identified. three of these clones manifested acetoacetyl coenzyme a reductase activity, the second enz ... | 1988 | 3049530 |
| role of hydrogen in the activation and regulation of hydrogen oxidation by the soluble hydrogenase from alcaligenes eutrophus h16. | the activation kinetics of the h2-oxidizing activity of the soluble hydrogenase from alcaligenes eutrophus h16 were investigated. activation with na2s2o4 plus 101 kpa h2 resulted in a rapid increase in activity over 1 h and constant activity after 3 h incubation. less-stable activations were achieved if enzyme was incubated with na2s2o4 under 1 kpa h2 or 101 kpa n2. the enzyme could also be partly activated either with nadh alone or with h2 alone. the level of activity obtained with both 101 kpa ... | 1988 | 3052435 |
| reversible and irreversible effects of nitric oxide on the soluble hydrogenase from alcaligenes eutrophus h16. | the effects of no on the h2-oxidizing and diaphorase activities of the soluble hydrogenase from alcaligenes eutrophus h16 were investigated. with fully activated enzyme, no (8-150 nm in solution) inhibited h2 oxidation in a time- and no-concentration-dependent process. neither h2 nor nad+ appeared to protect the enzyme against the inhibition. loss of activity in the absence of an electron acceptor was about 10 times slower than under turnover conditions. the inhibition was partially reversible; ... | 1988 | 3052436 |
| duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in alcaligenes eutrophus jmp134(pjp4). | the alcaligenes eutrophus jmp134 plasmid pjp4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-d) and 3-chlorobenzoic acid. tfda encodes 2,4-d monooxygenase, the initial enzyme in the 2,4-d catabolic pathway. the tfda locus has recently been localized to a region on pjp4 13 kilobases away from a cluster of five genes, tfdb to tfdf, which encode the enzymes responsible for the further degradation of 2,4-d to chloromaleylacetic acid (w.r. streber, k. n. ... | 1988 | 3056910 |
| microbial hydrolysis of organic nitriles and amides. | nitrile-hydrating enzymes produced by bacteria and fungi catalyse the conversion of a large number of chemically diverse nitriles, including many economically important compounds used industrially for chemical synthesis of amides and acids. this paper presents data on two new, highly different nitrile-hydrolysing enzymes which were isolated in connection with our studies on enzymic nitrile transformations. particular attention was paid to the enzymes' substrate specificities and sensitivity to s ... | 1988 | 3073055 |
| [retention of tined electrodes complicated by infection--a case report on its removal under total cardiopulmonary bypass]. | 1988 | 3074211 | |
| susceptibility of 310 nonfermentative gram-negative bacteria to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin. | the susceptibility of 310 mainly clinically isolated gram-negative nonfermenters to aztreonam, carumonam, ciprofloxacin, ofloxacin and fleroxacin was determined by agar dilution. all 3 quinolones were very active, with mic90 ranges (micrograms/ml) against all organisms ranging between 0.25 and 8 for ciprofloxacin, and 0.25 and 16.0 for ofloxacin and fleroxacin, respectively. aztreonam and carumonam showed less activity than the quinolones on a weight-for-weight basis, with mic90 values ranging f ... | 1988 | 3127127 |
| postoperative prophylaxis with norfloxacin in patients requiring bladder catheters. | the effect of once daily doses of 200 mg oral norfloxacin on the occurrence of catheter-associated bacteriuria (greater than 1000 cfu/ml) and pyuria was studied in 105 post-operative gynaecologic patients. norfloxacin was given from the second day after surgery until catheter removal. bacteriuria developed in 32 of 51 (63%) control patients compared to 8 of 54 (15%) patients receiving norfloxacin (p less than 0.001). pyuria was present in 22 of 51 (43%) control subjects versus only 3 of 54 (5%) ... | 1988 | 3141155 |
| differentiation of some gram-negative glucose nonfermenting bacteria using miniaturized carbon sources assimilation tests. | in water and soil the gram-negative nonfermenting bacteria play an important role in the biological mineralization process. to improve the methods for species differentiation of these heterogenous bacterial group, a total of 481 reference strains of gram-negative glucose nonfermenting bacteria belonging to the genera pseudomonas, alcaligenes, bordetella, agrobacterium, moraxella, acinetobacter, flavobacterium and some cdc groups have been investigated for their ability to utilize 42 different ca ... | 1988 | 3142164 |
| microbial transformation of the pyrethroid insecticides: permethrin, deltamethrin, fastac, fenvalerate, and fluvalinate. | pure cultures of bacillus cereus, pseudomonas fluorescens, and achromobacter sp. were shown to transform five pyrethroid insecticides in the presence of tween 80. one of the major products, 3-phenoxybenzoic acid, was further transformed to 4-hydroxy-3-phenoxybenzoic acid. permethrin was the most rapidly transformed of the pyrethroids, with a half-life of less than 5 days. | 1988 | 3145715 |
| display of the composition of polychlorinated biphenyls. | 1988 | 3147607 | |
| nucleotide homology and organization of chlorocatechol oxidation genes of plasmids pjp4 and pac27. | the 2,4-dichlorophenoxyacetate (2,4-d) catabolic plasmid pjp4 of alcaligenes eutrophus jmp134 contains two sets of nonidentical chlorocatechol oxidation gene sequences physically separated by a 7 kb dna region. we determined the nucleotide sequence of the 1.6 kb hindiii fragment containing the known genes tfdc and tfdd (don et al. 1985) which encode pyrocatechase and cycloisomerase, respectively. the 1.3 kb bglii-hindiii segment of recombinant plasmid pdc25 containing at least three chlorocatech ... | 1988 | 2830460 |
| size of diffusion pore of alcaligenes faecalis. | the diffusion pore of the outer membrane of alcaligenes faecalis was shown to be substantially smaller than the escherichia coli porin pore. in experiments with intact cells, pentoses and hexoses penetrated into the nacl-expanded periplasm, whereas saccharides of mr greater than 342 did not. cells treated with 0.5 m saccharides of mr greater than 342 weighed 33 to 38% less than cells treated with isotonic solution, suggesting that these saccharides do not permeate through the outer membrane. the ... | 1988 | 2835003 |
| bacterial collagenase and collagen identification. | a method is described for the cleavage of collagenous molecules by bacterial collagenase in the presence of sodium dodecyl sulphate (sds) and urea. comparison of three commercially available preparations of bacterial collagenase showed that the most efficient cleavage under these conditions was by the enzyme isolated from achromobacter iophagus (e.c. 3.4.24.8). no non-specific proteinase activity was apparent in conditions where all collagen types showed some susceptibility to attack. this metho ... | 1988 | 2835204 |
| near stoichiometric, irreversible inactivation of bacterial collagenases by o-chloranil (3,4,5,6-tetrachloro-1,2-benzoquinone). | the hydrogen-abstracting quinone derivative 3,4,5,6-tetrachloro-1,2-benzoquinone (o-chloranil) caused a strong, near stoichiometric, irreversible inactivation of the collagenases from bacillus cereus, clostridium histolyticum and achromobacter iophagus. p-chloranil was a weaker inactivator. o-chloranil reacted rapidly with a site that affected substrate binding. amino acid analyses of native and totally inactivated enzymes, and the ph-profile of inactivation suggest that the dissociated form of ... | 1988 | 2837215 |
| 1h nuclear magnetic resonance study of the protonation behaviour of the histidine residues and the electron self-exchange reaction of azurin from alcaligenes denitrificans. | the proton nuclear magnetic resonance spectrum of azurin from alcaligenes denitrificans at ph 6.0 and 309 k is reported. proton signals from all methionine and histidine residues (among them the copper ligands) have been assigned. the data have been used to study the ph behaviour of his35 and to establish the electron self-exchange rate of the protein. his35 appears to be protonated at ph less than 4.5, possibly after rupture of a salt bridge. no effects of this protonation on the tertiary struc ... | 1988 | 2837576 |
| isolation and characterization of a new plasmid from a flavobacterium sp. which carries the genes for degradation of 2,4-dichlorophenoxyacetate. | a flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-d), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, prc10. cured strains of the flavobacterium sp. lost the plasmid as well as the ability to degrade these chlorinated compounds. comparison of this plasmid with the well-characterized 2,4-d-degradative plasmid pjp4 from alcaligenes eutrophus showed regions of homology between the two ... | 1988 | 2842290 |
| separate binding sites for antimycin and mucidin in the respiratory chain of the bacterium paracoccus denitrificans and their occurrence in other denitrificans bacteria. | by means of the method of fluorimetric titration it has been shown that mucidin does not affect the attachment of antimycin to membranes from anaerobically grown paracoccus denitrificans. the fluorimetric titration with antimycin can be used in the determination of the amount of the cytochrome bc1 complex in the membrane. in cells inhibited with antimycin, the oxidation of cytochromes c was accompanied by the reduction of cytochrome b; in the presence of mucidin this effect did not take place. t ... | 1988 | 2844159 |
| nitric oxide complex of cytochrome c' in cells of denitrifying bacteria. | electron paramagnetic resonance (epr) spectra at 77k have been measured for the dried cells of three groups of chemoheterotrophic bacteria cultured in the presence of nitrate: i, denitrifying bacteria containing cytochrome c' (alcaligenes sp. ncib 11015, achromobacter xylosoxidans gifu 543); ii, denitrifying bacteria in which cytochrome c' has not been found (alcaligenes faecalis iam 1015, bacillus firmus nias 237, pseudomonas stutzeri atcc 17641); iii, non-denitrifying bacterium containing cyto ... | 1988 | 2844743 |
| catabolic instability, plasmid gene deletion and recombination in alcaligenes sp. br60. | an alcaligenes sp. br60, isolated from surface runoff waters of the hyde park industrial landfill, contained a novel 85 kb catabolic plasmid (pbr60) functional in 3-chlorobenzoate (3cba) degradation. the plasmid exhibited a spontaneous 3.2% frequency of deletion of a 14 kb fragment specifying 3cba degradation. the deletion mutant br 40 and mitomycin c cured strains were not able to grow on 3cba and had reversion frequencies of less than 10(-10) cell-1 generation-1. transformation or conjugation ... | 1988 | 2845877 |
| alcohol dehydrogenase gene from alcaligenes eutrophus: subcloning, heterologous expression in escherichia coli, sequencing, and location of tn5 insertions. | the nucleotide sequence of the gene that encodes the fermentative, multifunctional alcohol dehydrogenase (adh) in alcaligenes eutrophus, and of adjacent regions on a 1.8-kilobase-pair psti fragment was determined. from the deduced amino acid sequence, a molecular weight of 38,549 was calculated for the adh subunit. the amino acid sequence reveals homologies from 22.3 to 26.3% with zinc-containing alcohol dehydrogenases from eucaryotic organisms (schizosaccharomyces pombe, zea mays, mouse, horse ... | 1988 | 2846513 |
| a spectrophotometric assay for dissimilatory nitrite reductases. | a spectrophotometric assay for dissimilatory nitrite reductases has been developed utilizing mammalian cytochrome c (equine heart) as reductant and spectrophotometric agent. the copper-containing nitrite reductase from achromobacter cycloclastes has been shown to have apparent km's for reduced cytochrome c and nitrite of 86 +/- 5 and 5.63 +/- 0.03 microm, respectively. the heme cd-containing enzyme from pseudomonas stutzeri was shown to have apparent km's for reduced cytochrome c and nitrite of ... | 1988 | 2847585 |
| cloning of the alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (phb) and synthesis of phb in escherichia coli. | eight mutants of alcaligenes eutrophus defective in the intracellular accumulation of poly-beta-hydroxybutyric acid (phb) were isolated after transposon tn5 mutagenesis with the suicide vector psup5011. ecori fragments which harbor tn5-mob were isolated from phc79 cosmid gene banks. one of them, ppt1, was used as a probe to detect the intact 12.5-kilobase-pair ecori fragment pp1 in a lambda l47 gene bank of a. eutrophus genomic dna. in six of these mutants (psi, api, gpi, gpiv, gpv, and gpvi) th ... | 1988 | 2848014 |
| a novel vector allowing the expression of genes in a wide range of gram-negative bacteria. | the construction and use of a novel vector allowing the expression of genes in a wide range of gram-negative bacteria is described. the vector utilizes the regulatory region from is50. the 70-bp promoter region was isolated from one of the terminal inverted repeats of tn5 by creating ecori and sa/i or psti restriction sites by in vitro mutagenesis. this 70-bp region was shown to direct the expression of cat and lacz genes in different bacterial genera including alcaligenes, enterobacter cloacae, ... | 1988 | 2853690 |
| [the biogenetic interrelationships between flavins and vitamin b12 in achromobacter cobalamini]. | 1988 | 2854877 | |
| [microflora of the drinking water regenerated from atmospheric moisture condensate in hermetically sealed quarters]. | in an enclosed environment the species composition of microorganisms was quantified and metabolic processes in microbial cells isolated from the atmospheric condensate and reclaimed water were investigated. it was demonstrated that indicator microorganisms isolated from water and condensate samples (alcaligenes faecalis, citrobacter freundii, aeromonas hydrophila, acinetobacter-moraxella) were specific and differed from indicator microorganisms typical of regular tap water. | 1988 | 2898035 |
| outbreak of infection with achromobacter xylosoxidans from contaminated intravascular pressure transducers. | achromobacter xylosoxidans contaminating transducers caused 15 cases of hospital infection. in the eight patients with bacteraemia the interval from inoculation to fever was an average of 6.6 days. all the infected patients recovered. computerization of laboratory records allowed retrieval of previous isolates, and review of clinical records focused the problem on patients with cardiac and aortic diseases. the problem arose from the re-use of disposable equipment after disinfection with a benzal ... | 1988 | 2905368 |
| production and purification of d-aminoacylase from alcaligenes denitrificans and taxonomic study of the strain. | a d-aminoacylase-producing microorganism, strain da181, isolated from soil was identified as alcaligenes denitrificans subsp. denitrificans. this strain produced about 29,300 units (micromoles of product formed per hour) of d-aminoacylase and 2,300 units of l-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1% n-acetyl-dl-leucine as the carbon source. the d-aminoacylase was purified 345-fold. the specific activity of the purified enzyme was 108,600 units per mg ... | 1988 | 16347618 |
| production of d-aminoacylase from alcaligenes denitrificans subsp. xylosoxydans mi-4. | a bacterial strain that produces d-aminoacylase was isolated from soil and identified as alcaligenes denitrificans subsp. xylosoxydans mi-4. l-aminoacylase activity in this strain was only 1 to 2% of d-aminoacylase activity. d-aminoacylase was inducibly produced. n-acetyl-dl-leucine was the best inducer, and the d-isomer had the ability to induce the enzyme. enzymatic resolution of n-acetyl-dl-methionine with the crude enzyme was carried out, and the d/l ratio in the resolved methionine was appr ... | 1988 | 16347776 |
| transfer and expression of lithoautotrophy and denitrification in a host lacking these metabolic activities. | the conjugative 450-kilobase-pair megaplasmid phg1 from alcaligenes eutrophus h16 was transferred to the herbicide-degrading soil bacterium a. eutrophus jmp134. this transfer was achieved by means of rp4 mobilization and a tn5-mob insertion provided in trans on the megaplasmid replicon. although kanamycin-resistant transconjugants also occurred with other gram-negative species such as rhizobium, agrobacterium, and thiobacteria, a. eutrophus jmp134 was the only recipient which stably maintained t ... | 1988 | 16347808 |
| an immobilized hydrogenase from alcaligenes eutrophus h-16. | alcaligenes eutrophus h-16 was grown in continuous culture under conditions which induced hydrogenase production. the hydrogenase enzyme was extracted, partially purified and immobilized on porous glass. this enzyme was then studied both in solution and in immobilized form as a possible candidate for a number of industrial applications. it proved to have a stability (storage and operational) which was highly temperature dependent. temperatures near freezing caused the enzyme to retain its activi ... | 1988 | 18584750 |
| biodegradation of asphalt cement-20 by aerobic bacteria. | seven gram-negative, aerobic bacteria were isolated from a mixed culture enriched for asphalt-degrading bacteria. the predominant genera of these isolates were pseudomonas, acinetobacter, alcaligenes, flavimonas, and flavobacterium. the mixed culture preferentially degraded the saturate and naphthene aromatic fractions of asphalt cement-20. a residue remained on the surface which was resistant to biodegradation and protected the underlying asphalt from biodegradation. the most potent asphalt-deg ... | 1989 | 16347928 |
| bacterial colonization of cod (gadus morhua l.) and halibut (hippoglossus hippoglossus) eggs in marine aquaculture. | aquaculture has brought about increased interest in mass production of marine fish larvae. problems such as poor egg quality and mass mortality of fish larvae have been prevalent. the intensive incubation techniques that often result in bacterial overgrowth on fish eggs could affect the commensal relationship between the indigenous microflora and opportunistic pathogens and subsequently hamper egg development, hatching, larval health, and ongrowth. little information about the adherent microflor ... | 1989 | 16347937 |
| survival and activity of a 3-chlorobenzoate-catabolic genotype in a natural system. | a chlorobenzoate-degrading alcaligenes strain, br60, was introduced to flowthrough lake microcosms and exposed to 3-chlorobenzoate (3cba) concentrations from 0 to 25 mum. a dna probe specific for br60 chlorobenzoate catabolic genes was used with the most probable number (mpn) technique to enumerate bacteria harboring this genetic information. this mpn-dna hybridization method combined with [u-c]3cba uptake rate measurements allowed the correlation of the size and activity of a specific catabolic ... | 1989 | 16347951 |
| nuclear magnetic resonance studies of poly(3-hydroxybutyrate) and polyphosphate metabolism in alcaligenes eutrophus. | the metabolic pathways of poly(3-hydroxybutyrate) (phb) and polyphosphate in the microorganism alcaligenes eutrophus h16 were studied by h, c, and p nuclear magnetic resonance (nmr) spectroscopy and by conventional analytical techniques. a. eutrophus cells accumulated two storage polymers of phb and polyphosphate in the presence of carbon and phosphate sources under aerobic conditions after exhaustion of nitrogen sources. the solid-state cross-polarization/magic-angle spinning c nmr spectroscopy ... | 1989 | 16348055 |
| a 2.0-a structure of the blue copper protein (cupredoxin) from alcaligenes faecalis s-6. | the structure of a blue copper protein, cupredoxin, from the potent denitrifying bacterium alcaligenes faecalis s-6, has been determined and refined against 2 a x-ray diffraction data. the agreement between observed and calculated structure factors is 0.159, and estimated errors in coordinates are 0.09-0.15 a. the protein folds in a beta sandwich similar to plastocyanin and azurin and includes features such as a "kink" and a "tyrosine loop" which have been noted previously for these proteins as ... | 1989 | 2909547 |
| bacteremia and urinary tract infection associated with cdc group vd biovar 2. | a case of urinary tract infection and bacteremia caused by cdc group vd biovar 2 in a 23-year-old woman with hodgkin's disease is described. this is the first report of cdc group vd biovar 2 isolated from a clinical specimen and considered as a pathogen. detailed antimicrobial susceptibility data are presented. | 1989 | 2913028 |
| phenoxyacetic acid degradation by the 2,4-dichlorophenoxyacetic acid (tfd) pathway of plasmid pjp4: mapping and characterization of the tfd regulatory gene, tfdr. | plasmid pjp4 enables alcaligenes eutrophus jmp134 to degrade 3-chlorobenzoate and 2,4-dichlorophenoxyacetic acid (tfd). plasmid pro101 is a derivative of pjp4 obtained by insertion of tn1721 into a nonessential region of pjp4. plasmid pro101 was transferred by conjugation to several pseudomonas strains and to a. eutrophus aeo106, a cured isolate of jmp134. aeo106(pro101) and some pseudomonas transconjugants grew on tfd. transconjugants with a chromosomally encoded phenol hydroxylase also degrade ... | 1989 | 2914848 |
| plasmid-determined inducible efflux is responsible for resistance to cadmium, zinc, and cobalt in alcaligenes eutrophus. | in alcaligenes eutrophus ch34, resistance to chromate is plasmid determined, inducible, and based on decreased net accumulation of the metal anion. plasmid-encoded resistances to zinc, cadmium, cobalt, and nickel are resulting from inducible, energy-dependent cation efflux systems. | 1989 | 2914875 |
| unusual resistance to cefuroxime by strains of alcaligenes faecalis. | 1989 | 2921091 | |
| microbial degradation of beta-chlorinated four-carbon aliphatic acids. | alcaligenes sp. strain cc1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. dehalogenation of alpha-chlorinated acids could be measured by using resting cells grown on all the different carbon sources, whereas dehalogenation of beta-chlorinated four-carbon acids ... | 1989 | 2921240 |
| properties of a pseudomonas stutzeri outer membrane channel-forming protein (nosa) required for production of copper-containing n2o reductase. | a protein (nosa) in the outer membrane of pseudomonas stutzeri that is required for copper to be inserted into n2o reductase has been extracted and purified to homogeneity. the purified protein could form channels in black lipid bilayers. like n2o reductase, nosa contained copper and was only made anaerobically. in contrast to n2o reductase, its synthesis was repressed by exogenous copper (but not by mn, co, ni, zn, or fe). also in contrast to n2o reductase, nosa homologs were not immunologicall ... | 1989 | 2467899 |
| metal ion uptake by a plasmid-free metal-sensitive alcaligenes eutrophus strain. | the divalent metal cations of zinc, cadmium, cobalt, nickel, and manganese are transported into cells of alcaligenes eutrophus strain ae104 by the energy-dependent magnesium transport system. chromate is transported by the sulfate uptake system. | 1989 | 2472382 |
| operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pjp4 and pac27. | alcaligenes eutrophus harboring plasmid pjp4 (strain jmp134) is capable of growing on both 2,4-dichlorophenoxyacetate (2,4-d) and 3-chlorobenzoate (3-cba), while pseudomonas putida carrying plasmid pac27 (strain ac867) can utilize only 3-cba as the sole carbon source. the tfdcdef operon of the pjp4 plasmid and the clcabd operon of plasmid pac27 each encode enzymes for the degradation of chlorocatechols (clc), key intermediates in the catabolism of 2,4-d and 3-cba. similarities in the nucleotide ... | 1989 | 2583528 |
| asp700i, a novel isoschizomer of xmni from achromobacter species 700 recognizing 5'-gaann/nnttc-3'. | 1989 | 2587236 | |
| asphi, a novel isoschizomer of hgiai from achromobacter species h recognizing 5'-gwgcw/c-3'. | 1989 | 2587281 | |
| fatal neonatal meningitis and ventriculitis caused by multiresistant achromobacter xylosoxidans. a case report. | meningitis and ventriculitis in a 6-day-old neonate caused by a gram-negative glucose-non-fermenting organism, achromobacter xylosoxidans, was resistant to most antibiotics except ceftazidime and imipenem. the organism became resistant after 28 days treatment with ceftazidime. when the infant was 7 weeks old, imipenem became available but, in spite of 3 days of intravenous treatment, the organism was still recovered from ventricular cerebrospinal fluid and the child died. this would appear to be ... | 1989 | 2588089 |
| antibacterial activity of norfloxacin against 1700 relatively resistant clinical isolates. | a total of 1724 clinical isolates from patients with different infections were tested in vitro to determine their susceptibility to norfloxacin. antibacterial activity of norfloxacin was compared with other drugs that were commonly used in the hospital. of the 919 strains of enterobacteriaceae tested, all except four isolates of enterobacter, two of serratia and one of proteus were susceptible to norfloxacin. ninety-five percent of the 199 strains of pseudomonas aeruginosa were also inhibited by ... | 1989 | 2598776 |
| evidence for a new pathway in the bacterial degradation of 4-fluorobenzoate. | six bacterial strains able to use 4-fluorobenzoic acid as their sole source of carbon and energy were isolated by selective enrichment from various water and soil samples from the stuttgart area. according to their responses in biochemical and morphological tests, the organisms were assigned to the genera alcaligenes, pseudomonas, and aureobacterium. to elucidate the degradation pathway of 4-fluorobenzoate, metabolic intermediates were identified. five gram-negative isolates degraded this substr ... | 1989 | 2604392 |
| bacterial colonization of domestic reverse-osmosis water filtration units. | we have analyzed the bacterial content of water from the reservoirs of 300 reverse-osmosis units installed in households. the heterotrophic plate counts on r2a medium (20 and 35 degrees c) ranged from 0 to 10(7) colony forming units per millilitre (cfu/ml). most reservoirs contained water with bacterial counts between 10(4) and 10(5) cfu/ml. the bacteria identified were pseudomonas (not aeruginosa), alcaligenes or moraxella, acinetobacter, flavobacterium, and chromobacterium. this report emphasi ... | 1989 | 2611732 |
| immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria. | polyclonal antibodies were used to identify heme or copper nitrite reductases in the following groups: 23 taxonomically diverse denitrifiers from culture collections, 100 numerically dominant denitrifiers from geographically diverse environments, and 51 denitrifiers from a culture collection not selected for denitrification. antisera were raised against heme nitrite reductases from pseudomonas aeruginosa and pseudomonas stutzeri and against copper nitrite reductase from achromobacter cycloclaste ... | 1989 | 2624465 |
| effect of redox potential on the activation of the nad-dependent hydrogenase from alcaligenes eutrophus z1. | a formal kinetic treatment of the autocatalytic activation cycle of the nad-dependent hydrogenase from alcaligenes eutrophus z1 is presented. the value for the enzyme first-order activation rate constant is estimated to be (2.0 +/- 0.6) s-1 (ph 7.8, 25 degrees c). the effect of the redox potential on the activation properties of the nad-dependent hydrogenase is studied. hydrogenase activation is controlled by a midpoint redox potential of approximately -100 mv (ph 7.8). once activated the enzyme ... | 1989 | 2643385 |
| redox-dependent inactivation of the nad-dependent hydrogenase from alcaligenes eutrophus z1. | a novel inactivation mechanism of the nad-dependent hydrogenase from alcaligenes eutrophus z1 comprising redox-dependent steps is described. the model of the hydrogenase inactivation process is proposed which implies that the enzyme may exist in several forms which differ in their stability and spectral properties. one of these forms, existing within a limited (approximately -200 +/- 30 mv) potential range, undergoes a rapid and irreversible inactivation. the dissociation of the fmn prosthetic g ... | 1989 | 2643386 |
| cloning, nucleotide sequence, and expression in escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from alcaligenes faecalis. | the extracellular poly(3-hydroxybutyrate) depolymerase gene from alcaligenes faecalis t1 was cloned into escherichia coli dh1 by using the plasmid puc8. an a. faecalis t1 genomic library was prepared in e. coli from a partial sau3ai digest and screened with antibody against the depolymerase. of the 29 antibody-positive clones, 1 (pdp14), containing about 4 kilobase pairs of a. faecalis t1 dna, caused expression of a high level of depolymerase activity in e. coli. the enzyme purified from e. coli ... | 1989 | 2644188 |
| genetic determinants of a nickel-specific transport system are part of the plasmid-encoded hydrogenase gene cluster in alcaligenes eutrophus. | nickel-deficient (nic-) mutants of alcaligenes eutrophus requiring high levels of nickel ions for autotrophic growth with hydrogen were characterized. the nic- mutants carried defined deletions in the hydrogenase gene cluster of the indigenous phg megaplasmid. nickel deficiency correlated with a low level of the nickel-containing hydrogenase activity, a slow rate of nickel transport, and reduced activity of urease. the nic+ phenotype was restored by a cloned dna sequence (hoxn) of a megaplasmid ... | 1989 | 2646280 |
| cloning of a carbofuran hydrolase gene from achromobacter sp. strain wm111 and its expression in gram-negative bacteria. | a 14-kilobase-pair (kbp) ecori dna fragment that encodes an enzyme capable of rapid hydrolysis of n-methylcarbamate insecticides (carbofuran hydrolase) was cloned from carbofuran-degrading achromobacter sp. strain wm111. when used to probe southern blots containing plasmid and total dnas from wm111, this 14-kbp fragment hybridized strongly to a 14-kbp ecori fragment from the greater than 100-kbp plasmid harbored by this strain but weakly to ecori-digested total dna from achromobacter sp. strain ... | 1989 | 2661544 |
| [catalytic component of calmodulin-independent adenylate cyclase from bovine brain. isolation and determination of partial amino acid sequence]. | the catalytic component of calmodulin-independent adenylate cyclase of cattle cerebral cortex was solubilized and purified to the homogeneous state. the conditions for preparative obtaining of the enzyme on the column with immobilized antibodies to adenylate cyclase were found. these antibodies were proved to interact with the calmodulin-independent rather than the calmodulin-dependent form of the enzyme. molecular mass of the calmodulin-independent adenylate cyclase determined electrophoretical ... | 1989 | 2662976 |
| poly-beta-hydroxybutyrate biosynthesis in alcaligenes eutrophus h16. characterization of the genes encoding beta-ketothiolase and acetoacetyl-coa reductase. | the poly-beta-hydroxybutyrate biosynthetic thiolase gene from zoogloea ramigera was used as a hybridization probe to screen restriction digests of alcaligenes eutrophus h16 dna. specific hybridization signals were obtained and two fragments (a 2.3-kilobase psti fragment and a 15-kilobase ecori fragment) cloned in the escherichia coli vector puc8 (plasmids paet3/paet10 and paet29, respectively). biochemical analysis of lysates of e. coli cells containing each plasmid identified significant levels ... | 1989 | 2670935 |
| poly-beta-hydroxybutyrate (phb) biosynthesis in alcaligenes eutrophus h16. identification and characterization of the phb polymerase gene (phbc). | the phbc gene encoding the third enzyme of the poly-beta-hydroxybutyrate biosynthetic pathway, poly-beta-hydroxybutyrate polymerase, in alcaligenes eutrophus h16 has been identified by the complementation of poly-beta-hydroxybutyrate negative mutants of a. eutrophus h16. these results demonstrate that the three enzymes of the poly-beta-hydroxybutyrate biosynthetic pathway are organized phbc-phba-phbb. expression of all three genes in escherichia coli results in a significant level (50% dry cell ... | 1989 | 2670936 |
| laboratory survey of fluoroquinolone activity. | fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ... | 1989 | 2672262 |
| conduritol aziridine: a new mechanism-based glucosidase inactivator. | a new mechanism-based glucosidase inactivator, conduritol aziridine (1,2-dideoxy-1,2-epimino-myo-inositol), has been synthesised from myo-inositol. this aziridine inactivates both the beta-glucosidase from alcaligenes faecalis and the alpha-glucosidase from yeast according to the expected pseudo-first order kinetics. inactivation constants measured are ki = 3.0mm, ki = 0.077 min-1 for the beta-glucosidase, and ki = 9.5mm, ki = 0.39 min-1 for the alpha-glucosidase. evidence for irreversible inact ... | 1989 | 2673241 |
| structural organization of the membrane-bound hydrogenase isolated from alcaligenes eutrophus as revealed by electron microscopy. | electron microscopy of negatively stained samples of the membrane-bound hydrogenase isolated from alcaligenes eutrophus was used to obtain enzyme images with an estimated resolution of 2.5 nm. the two subunits with shapes similar to the letter 'u' making up the enzyme could be seen to be joined in two planes orthogonal to each other, making contact with their concave sides. in face-on view, the particle exhibited bilateral symmetry. | 1989 | 2673917 |
| expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from alcaligenes eutrophus. | resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pmol30 in alcaligenes eutrophus results from a cation efflux system. five membrane-bound polypeptides that were expressed in escherichia coli from this determinant under the control of a phage t7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. the contributions of the polypeptides to the cation efflux syste ... | 1989 | 2678100 |
| cloning, nucleotide sequence, and expression of achromobacter protease i gene. | achromobacter protease i (api) is a lysine-specific serine protease which hydrolyzes specifically the lysyl peptide bond. a gene coding for api was cloned from achromobacter lyticus m497-1. nucleotide sequence of the cloned dna fragment revealed that the gene coded for a single polypeptide chain of 653 amino acids. the n-terminal 205 amino acids, including signal peptide and the threonine/serine-rich c-terminal 180 amino acids are flanking the 268 amino acid-mature protein which was identified b ... | 1989 | 2684982 |
| [electrophoretic analysis of the exoproducts of bacteria in the genus pseudomonas]. | a high degree of similarity (exceeding 90%) between the electrophoregrams of the exoproducts of 31 pseudomonas strains, including the standard species p. aeruginosa, p. maltophilia, p. putida, p. alcaligenes, p. testosteroni, p. diminuta, p. stutseri, p. fluorescens, has been shown by the method of electrophoresis in polyacrylamide gel. | 1989 | 2686300 |
| the sodium cycle: a novel type of bacterial energetics. | the progress of bioenergetic studies on the role of na+ in bacteria is reviewed. experiments performed over the past decade on several bacterial species of quite different taxonomic positions show that na+ can, under certain conditions, substitute for h+ as the coupling ion. various primary na+ pumps (delta mu na+ generators) are described, i.e., na+ -motive decarboxylases, nadh-quinone reductase, terminal oxidase, and atpase. the delta mu na+ formed is shown to be consumed by na+ driven atp-syn ... | 1989 | 2687258 |
| respiratory na+ pump and na+-dependent energetics in vibrio alginolyticus. | the marine bacterium vibrio alginolyticus was found to possess the respiratory na+ pump that generates an electrochemical potential of na+, which plays a central role in bioenergetics of v. alginolyticus, as a direct result of respiration. mutants defective in the na+ pump revealed that one of the two kinds of nadh: quinone oxidoreductase requires na+ for activity and functions as the na+ pump. the na+ pump composed of three subunits was purified and reconstituted into liposomes. generation of m ... | 1989 | 2687261 |