Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| sepsis-related mortality of very low birth weight brazilian infants: the role of pseudomonas aeruginosa. | the aim of this study is to identify risk factors for sepsis-related mortality in low birth weight (<1500 g) infants. we performed retrospective cohort study to investigate risk factors for sepsis-related mortality in all neonates birth weight <1500 g admitted to level iii neonatal intensive care unit, brazil, april 2001/september 2004. of the 203 cases, 71 (35%) had sepsis. of those, gram-positive was identified in 52/87 blood cultures (59.8%), the most common coagulase-negative staphylococcus ... | 2010 | 20182631 |
| sepsis-related mortality of very low birth weight brazilian infants: the role of pseudomonas aeruginosa. | the aim of this study is to identify risk factors for sepsis-related mortality in low birth weight (<1500 g) infants. we performed retrospective cohort study to investigate risk factors for sepsis-related mortality in all neonates birth weight <1500 g admitted to level iii neonatal intensive care unit, brazil, april 2001/september 2004. of the 203 cases, 71 (35%) had sepsis. of those, gram-positive was identified in 52/87 blood cultures (59.8%), the most common coagulase-negative staphylococcus ... | 2010 | 20182631 |
| heterotrophic nitrogen removal by a newly isolated acinetobacter calcoaceticus hnr. | strain hnr, isolated from a membrane bioreactor (mbr), demonstrates a surprising ability to convert ammonium to nitrogen gas under aerobic conditions while growing heterotrophically. on the basis of phylogenetic analysis of the 16s rrna gene sequence, strain hnr was related to acinetobacter calcoaceticus (98.9% identity). nitrogen balance during heterotrophic growth with 120mg/l of nh(4)(+)-n showed that 40.2% of nh(4)(+)-n was in the form of n(2) and 52.1% was found in biomass. only a trace pro ... | 2010 | 20202830 |
| biological treatment of waste gas containing mixture of monochlorobenzene (mcb) and benzene in a bench scale biofilter. | the paper outlines treatment of waste gas containing monochlorobenzene (mcb) and benzene in a mixture using biofilter packed with compost and woodchips seeded with acinetobacter calcoaceticus. the biofilter could treat waste gas containing mcb and benzene effectively with an efficiency of (99+/-5%) and (97+/-6%) at optimal empty bed contact time (ebct) of 3 min with a loading of 57 g/m(3)/h of mcb and 2g/m(3)/h of benzene. at optimum loading of mcb and benzene, the biofilter showed total bacteri ... | 2010 | 20202831 |
| rapid differentiation of francisella species and subspecies by fluorescent in situ hybridization targeting the 23s rrna. | francisella (f.) tularensis is the causative agent of tularemia. due to its low infectious dose, ease of dissemination and high case fatality rate, f. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. microbiological diagnosis is cumbersome and time-consuming. methods for the direct detection of the pathogen (immunofluorescence, pcr) have been developed but are restricted to reference laboratories. | 2010 | 20205957 |
| acinetobacter brisouii sp. nov., isolated from a wetland in korea. | a bacterial strain 5yn5-8(t) was isolated from peat layer on yongneup in korea. cells of strain 5yn5-8(t) were strictly aerobic, gram-negative, coccobacilli, non-spore forming, and non-motile. the isolate exhibited optimal growth at 28 degrees c, ph 7.0, and 0-1% nacl. results of 16s rrna gene sequence analyses indicated a close relationship of this isolate to acinetobacter calcoaceticus (97.8% similarity for strain dsm 30006(t)). it also exhibited 94.4-97.8% 16s rrna gene sequence similarities ... | 2010 | 20221727 |
| isolation and characterization of a mutant of the marine bacterium alcanivorax borkumensis sk2 defective in lipid biosynthesis. | in many microorganisms, the key enzyme responsible for catalyzing the last step in triacylglycerol (tag) and wax ester (we) biosynthesis is an unspecific acyltransferase which is also referred to as wax ester synthase/acyl coenzyme a (acyl-coa):diacylglycerol acyltransferase (ws/dgat; atfa). the importance and function of two atfa homologues (atfa1 and atfa2) in the biosynthesis of tags and wes in the hydrocarbon-degrading marine bacterium alcanivorax borkumensis sk2 have been described recently ... | 2010 | 20305021 |
| rhamnolipids: diversity of structures, microbial origins and roles. | rhamnolipids are glycolipidic biosurfactants produced by various bacterial species. they were initially found as exoproducts of the opportunistic pathogen pseudomonas aeruginosa and described as a mixture of four congeners: alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate (rha-rha-c(10)-c(10)), alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-beta-hydroxydecanoate (rha-rha-c(10)), as well as their mono-rhamnolipid congeners rha-c(10)-c(10) and rha-c(10). ... | 2010 | 20336292 |
| [biodegradation of surface-active substances of acinetobacter calcoaceticus k-4]. | a capacity of microorganisms of different taxonomic groups to assimilate surface-active substances (surfactants) of acinetobacter calcoaceticus k-4 as a single source of carbon and energy has been established. it was shown that a. calcoaceticus k-4 cannot use its own surfactants as a source of carbon nutrition. the use of biocide formalin in concentration of 0.1% permits to prolong the term of preservation of a. calcoaceticus k-4 surfactants to 3.5 months without a loss of their surfactant and e ... | 2010 | 20364713 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| molecular epidemiology of acinetobacter baumannii-acinetobacter calcoaceticus complex isolated from clinical specimens at an intensive care unit. | 2010 | 20383429 | |
| oxa-24 carbapenemase gene flanked by xerc/xerd-like recombination sites in different plasmids from different acinetobacter species isolated during a nosocomial outbreak. | a clinical strain of acinetobacter calcoaceticus resistant to carbapenems was isolated from a blood culture sample from an inpatient in a hospital in madrid (spain) during a large outbreak of infection (affecting more than 300 inpatients), caused by a multidrug-resistant acinetobacter baumannii clone. the carbapenem resistance in both the a. calcoaceticus and a. baumannii clones was due to a bla(oxa-24) gene harbored in different plasmids. the plasmids were fully sequenced, revealing the presenc ... | 2010 | 20385865 |
| effect of microwave irradiation on cellular disintegration of gram positive and negative cells. | this research investigated the effect of microwave irradiation (mwi) on cell disintegration in municipal secondary sludge (mss). a representative mss gram-positive bacterium (bacillus subtilis) and gram-negative bacteria (acinetobacter calcoaceticus and pseudomonas aeruginosa) were pure cultured separately and treated using mwi. compared to untreated controls, mwi significantly increased the soluble chemical oxygen demand (cod) (1.8-4.0-fold), soluble protein concentration (1.1-1.8-fold), and so ... | 2010 | 20393700 |
| adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria. | this study was performed in order to characterize the relationship between adhesion and biofilm formation abilities of drinking water-isolated bacteria (acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staphylococcus sp.). adhesion was assessed by two distinct methods: thermodynamic prediction of adhesion potential by quantifying hydrophobicity and the free energy of adhesion; and by microtiter plate assays. biofilms w ... | 2010 | 20405208 |
| iron regulated outer membrane proteins (iromps) as potential targets against carbapenem-resistant acinetobacter spp. isolated from a medical centre in malaysia. | carbapenem-resistant acinetobacter spp. have gained increasing significance as opportunistic pathogens in hospitalized patients. carbapenem resistance is often associated with the loss and/or decrease in outer membrane proteins (omp) and overexpression of multidrug efflux systems. however, carbapenem-hydrolysing beta-lactamases of ambler class b (metallo-enzymes) and ambler class d (oxacillinases) have also been detected in acinetobacter spp. in this study we have investigated the role of the ir ... | 2010 | 20424311 |
| isolation and characterization of a virulent bacteriophage ab1 of acinetobacter baumannii. | acinetobacter baumannii is an emerging nosocomial pathogen worldwide with increasing prevalence of multi-drug and pan-drug resistance. a. baumannii exists widely in natural environment, especially in health care settings, and has been shown difficult to be eradicated. bacteriophages are often considered alternative agent for controlling bacterial infection and contamination. in this study, we described the isolation and characterization of one virulent bacteriophage ab1 capable of specifically i ... | 2010 | 20426877 |
| clinical experience with tigecycline in the treatment of carbapenem-resistant acinetobacter infections. | tigecycline is a promising therapeutic option against many current multidrug resistant pathogens. the aim of this retrospective study was to determine the clinical and microbiological outcomes of patients treated with tigecycline for serious infections caused by carbapenem-resistant acinetobacter calcoaceticus-acinetobacter baumannii complex (acb-complex). a retrospective study was conducted to define the patients who received tigecycline for carbapenem-resistant acb-complex infections between 1 ... | 2010 | 20435570 |
| rt-pcr and statistical analyses of adeabc expression in clinical isolates of acinetobacter calcoaceticus-acinetobacter baumannii complex. | the relationship between expression of adeabc and minimal inhibitory concentration (mic) of tigecycline was investigated by rt-pcr and statistical analyses in a population of 106 clinical isolates (mic range, 0.0313-16 microg/ml) of acinetobacter calcoaceticus-acinetobacter baumannii complex. there was a statistically significant linear relationship (p < 0.0001) between log-transformed expression values and log-transformed mic values, indicating that overexpression of adeabc efflux pump is a pre ... | 2010 | 20438348 |
| a distinct dgat with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in euonymus and transgenic seeds. | endosperm and embryo tissues from the seeds of euonymus alatus (burning bush) accumulate high levels of 3-acetyl-1,2-diacyl-sn-glycerols (actags) as their major storage lipids. in contrast, the aril tissue surrounding the seed produces long-chain triacylglycerols (lctags) typical of most other organisms. the presence of the sn-3 acetyl group imparts actags with different physical and chemical properties, such as a 30% reduction in viscosity, compared to lctags. comparative transcriptome analysis ... | 2010 | 20439724 |
| lipid droplet-associated proteins are involved in the biosynthesis and hydrolysis of triacylglycerol in mycobacterium bovis bacillus calmette-guerin. | mycobacteria store triacylglycerols (tgs) in the form of intracellular lipid droplets (lds) during hypoxia-induced nonreplicating persistence. these bacteria are phenotypically drug-resistant and therefore are believed to be the cause for prolonged tuberculosis treatment. lds are also associated with bacilli in tuberculosis patient sputum and hypervirulent strains. although proteins bound to lds are well characterized in eukaryotes, the identities and functions of such proteins have not been des ... | 2010 | 20452973 |
| enhanced surface colonization by escherichia coli o157:h7 in biofilms formed by an acinetobacter calcoaceticus isolate from meat-processing environments. | a meat factory commensal bacterium, acinetobacter calcoaceticus, affected the spatial distribution of escherichia coli o157:h7 surface colonization. the biovolume of e. coli o157:h7 was 400-fold higher (1.2 x 10(6) microm(3)) in a dynamic cocultured biofilm than in a monoculture (3.0 x 10(3) microm(3)), and e. coli o157:h7 colonized spaces between a. calcoaceticus cell clusters. | 2010 | 20453142 |
| adsorption of extracellular chromosomal dna and its effects on natural transformation of azotobacter vinelandii. | to better understand the influence of environmental conditions on the adsorption of extracellular chromosomal dna and its availability for natural transformation, the amount and conformation of adsorbed dna were monitored under different conditions in parallel with transformation assays using the soil bacterium azotobacter vinelandii. dna adsorption was monitored using the technique of quartz crystal microbalance with dissipation (qcm-d). both silica and natural organic matter (nom) surfaces wer ... | 2010 | 20453151 |
| multilocus sequence types of carbapenem-resistant pseudomonas aeruginosa in singapore carrying metallo-beta-lactamase genes, including the novel bla(imp-26) gene. | nine imipenem-resistant pseudomonas aeruginosa isolates were found to contain a variety of metallo-beta-lactamase genes, including bla(imp-1), bla(imp-7), bla(vim-2), bla(vim-6), and the novel bla(imp-26). multilocus sequence typing showed a diversity of sequence types. comparison with isolates from an earlier study showed that the epidemic clones from 2000 have not become established. | 2010 | 20463166 |
| natural competence in thermoanaerobacter and thermoanaerobacterium species. | low-g+c thermophilic obligate anaerobes in the class clostridia are considered among the bacteria most resistant to genetic engineering due to the difficulty of introducing foreign dna, thus limiting the ability to study and exploit their native hydrolytic and fermentative capabilities. here, we report evidence of natural genetic competence in 13 thermoanaerobacter and thermoanaerobacterium strains previously believed to be difficult to transform or genetically recalcitrant. in thermoanaerobacte ... | 2010 | 20472726 |
| hexavalent molybdenum reduction to mo-blue by acinetobacter calcoaceticus. | a local molybdenum-reducing bacterium was isolated and tentatively identified as acinetobacter calcoaceticus strain dr.y12 based on carbon utilization profiles using biolog gn plates and 16s rdna comparative analysis. molybdate reduction was optimized under conditions of low dissolved oxygen (37 degrees c and ph 6.5). of the electron donors tested, glucose, fructose, maltose and sucrose supported molybdate reduction after 1 d of incubation, glucose and fructose supporting the highest mo-blue pro ... | 2010 | 20490756 |
| evaluation of the analytical performance of the xpert mtb/rif assay. | we performed the first studies of analytic sensitivity, analytic specificity, and dynamic range for the new xpert mtb/rif assay, a nucleic acid amplification-based diagnostic system that detects mycobacterium tuberculosis and rifampin (rif) resistance in under 2 h. the sensitivity of the assay was tested with 79 phylogenetically and geographically diverse m. tuberculosis isolates, including 42 drug-susceptible isolates and 37 rif-resistant isolates containing 13 different rpob mutations or mutat ... | 2010 | 20504986 |
| natural transformation of vibrio cholerae as a tool--optimizing the procedure. | vibrio cholerae gains natural competence upon growth on chitin. this allows the organism to take up free dna from the environment and to incorporate it into its genome by homologous recombination. | 2010 | 20509862 |
| bacterial gut symbionts contribute to seed digestion in an omnivorous beetle. | obligate bacterial symbionts alter the diets of host animals in numerous ways, but the ecological roles of facultative bacterial residents that colonize insect guts remain unclear. carabid beetles are a common group of beneficial insects appreciated for their ability to consume insect prey and seeds, but the contributions of microbes to diet diversification in this and similar groups of facultative granivores are largely unknown. | 2010 | 20520762 |
| acinetobacter endocarditis presenting as a large right atrial mass: an atypical presentation. | this paper discusses a 26-year-old woman with end-stage renal disease on hemodialysis and acinetobacter calcoaceticus-baumannii complex endocarditis. the patient had an indwelling right internal jugular catheter that was probably the nidus of infection. transthoracic echocardiogram revealed an atypical presentation of the endocarditis as a large intracardiac mass, measuring in centimeters and occupying more than 50% of the right atrial cavity. the mass was attached to the lateral wall of the rig ... | 2010 | 20529103 |
| improvement of an e. coli bioreporter for monitoring trace amounts of phenol by deletion of the inducible sigma54-dependent promoter. | an escherichia coli bioreporter harboring the phenol-inducible mphk promoter (p(mphk)) from acinetobacter calcoaceticus phea-2 fused to a beta-galactosidase gene (lacz) and the regulator gene (mopr) of a. calcoaceticus ncib8250 was constructed to detect phenol. p(mphk) containing three inverted repeats (ir1, ir2 and ir3) upstream of mphk was activated by the regulator mopr in the presence of phenol. deletion analysis of p(mphk) revealed that ir2 and ir3 were essential for promoter activity, whil ... | 2010 | 20533077 |
| novel rhamnolipid biosurfactants produced by a polycyclic aromatic hydrocarbon-degrading bacterium pseudomonas aeruginosa strain ny3. | a novel rhamnolipid biosurfactant-producing and polycyclic aromatic hydrocarbon (pah)-degrading bacterium pseudomonas aeruginosa strain ny3 was isolated from petroleum-contaminated soil samples. strain ny3 was characterized by its extraordinary capacity to produce structurally diverse rhamnolipids. a total of 25 rhamnolipid components and 37 different parent molecular ions, representing various metal ion adducts (na(+), 2na(+) and k(+)), were detected by matrix-assisted laser desorption ionizati ... | 2010 | 20580808 |
| detection of human intestinal catalase-negative, gram-positive cocci by rrna-targeted reverse transcription-pcr. | an analytical system based on rrna-targeted reverse transcription-quantitative pcr (rt-qpcr) for enumeration of catalase-negative, gram-positive cocci was established. subgroup- or species-specific primer sets targeting 16s or 23s rrna from enterococcus, streptococcus, and lactococcus were newly developed. the rt-qpcr method using these primers together with the previously reported primer sets specific for the enterococcus genus, the streptococcus genus, and several streptococcus species was fou ... | 2010 | 20581195 |
| impact of direct soil exposures from airborne dust and geophagy on human health. | over evolutionary time humans have developed a complex biological relationship with soils. here we describe modes of soil exposure and their biological implications. we consider two types of soil exposure, the first being the continuous exposure to airborne soil, and the second being dietary ingestion of soils, or geophagy. it may be assumed that airborne dust and ingestion of soil have influenced the evolution of particular dna sequences which control biological systems that enable individual o ... | 2010 | 20617027 |
| molecular characterization and heterologous expression of quinate dehydrogenase gene from gluconobacter oxydans ifo3244. | the quinate dehydrogenase (qdh) from gluconobacter oxydans ifo3244 exhibits high affinity for quinate, suggesting its application in shikimate production. nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. the qdh gene has the unusual ttg translation initiation codon. conserved regions and a signature sequence for the quinoprotein family were observed. phylogenetic analysis demonstrated relatedness of qdh from g. oxydans to other qu ... | 2010 | 20618134 |
| recognition of potentially novel human disease-associated pathogens by implementation of systematic 16s rrna gene sequencing in the diagnostic laboratory. | clinical isolates that are difficult to identify by conventional means form a valuable source of novel human pathogens. we report on a 5-year study based on systematic 16s rrna gene sequence analysis. we found 60 previously unknown 16s rrna sequences corresponding to potentially novel bacterial taxa. for 30 of 60 isolates, clinical relevance was evaluated; 18 of the 30 isolates analyzed were considered to be associated with human disease. | 2010 | 20631113 |
| biochip system for rapid and accurate identification of mycobacterial species from isolates and sputum. | the accurate detection of mycobacterial species from isolates and clinical samples is important for pathogenic diagnosis and treatment and for disease control. there is an urgent need for the development of a rapid, simple, and accurate detection method. we established a biochip assay system, including a biochip, sample preparation apparatus, hybridization instrument, chip washing machine, and laser confocal scanner equipped with interpretation software for automatic diagnosis. the biochip simul ... | 2010 | 20686082 |
| influence of the diversity of bacterial isolates from drinking water on resistance of biofilms to disinfection. | single- and multispecies biofilms formed by six drinking water-isolated bacterial species were used to assess their susceptibilities to sodium hypochlorite (shc). in general, multispecies biofilms were more resistant to inactivation and removal than single biofilms. total biofilm inactivation was achieved only for acinetobacter calcoaceticus single-species biofilms and for those multispecies biofilms without a. calcoaceticus. biofilms with all bacteria had the highest resistance to shc, while th ... | 2010 | 20693444 |
| sustainable biodegradation of phenol by acinetobacter calcoaceticus p23 isolated from the rhizosphere of duckweed lemna aoukikusa. | phenol-degrading bacteria were isolated from the rhizosphere of duckweed (lemna aoukikusa) using an enrichment culture method. one of the isolates, p23, exhibited an excellent ability to degrade phenol and attach to a solid surface under laboratory conditions. phylogenetic analysis revealed that p23 belongs to the genera acinetobacter and has the highest similarity to acinetobacter calcoaceticus. p23 rapidly colonized on the surface of sterilized duckweed roots and formed biofilms, indicating th ... | 2010 | 20704249 |
| emergence and distribution of plasmids bearing the blaoxa-51-like gene with an upstream isaba1 in carbapenem-resistant acinetobacter baumannii isolates in taiwan. | the bla(oxa-51)-like gene with an upstream isaba1 (isaba1-bla(oxa-51)-like gene) was originally found on the chromosomes of carbapenem-resistant or -susceptible acinetobacter baumannii isolates. however, a plasmid-borne isaba1-bla(oxa-51)-like gene has recently been identified in acinetobacter genomic species 13tu and several a. baumannii isolates in taiwan, and all of the isolates are carbapenem resistant. this study aimed to characterize the plasmids bearing the isaba1-bla(oxa-51)-like gene an ... | 2010 | 20713680 |
| antibiotic producing potentials of three freshwater actinomycetes isolated from the eastern cape province of south africa. | crude extracts of three actinomycetes species belonging to saccharopolyspora (tr 046 and tr 039) and actinosynnema (tr 024) genera were screened for antibacterial activities against a panel of several bacterial strains. the extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (tr 046); 8 to15 mm (tr 039); and 10 to 13 mm (tr 024). the minimum inhibitory concentrations ranged from 0.078 to 10 mg/ml (tr 04 ... | 2010 | 20717525 |
| application of a novel method for optimization of bioemulsan production in a miniaturized bioreactor. | a novel and economical method was used to optimize bacterial growth and bioemulsan production. acinetobacter calcoaceticus ptcc 1641 was applied in an innovated synthetic medium comprised whey and mineral salt medium (msm) in ratio of 1:1 in a shaken flask bioreactor. the optimum inoculum size, shaking frequency rate and filling volume (v(l)) using ventilation flasks were investigated that resulted in 5 vol.%, 300 rpm and 15 ml), respectively. the surface tension decreased around 48% during bioe ... | 2010 | 20719500 |
| genotypic and phenotypic correlations of multidrug-resistant acinetobacter baumannii-a. calcoaceticus complex strains isolated from patients at the national naval medical center. | 2010 | 20739490 | |
| synthesis and spectrum of the neoglycoside achn-490. | achn-490 is a neoglycoside, or "next-generation" aminoglycoside (ag), that has been identified as a potentially useful agent to combat drug-resistant bacteria emerging in hospitals and health care facilities around the world. a focused medicinal chemistry campaign produced a collection of over 400 sisomicin analogs from which achn-490 was selected. we tested achn-490 against two panels of gram-negative and gram-positive pathogens, many of which harbored ag resistance mechanisms. unlike legacy ag ... | 2010 | 20805391 |
| global regulator h-ns and lipoprotein nlpi influence production of extracellular dna in escherichia coli. | extracellular dna (edna) is a structural component of the polymeric matrix of biofilms from different species. different mechanisms for dna release have been proposed including lysis of cells, lysis of dna-containing vesicles, and dna secretion. here, a genome-wide screen of 3985 non-lethal mutations was performed to identify genes whose deletion alters edna release in escherichia coli. deleting nlpi, yfec, and rna increased edna from planktonic cultures while deleting hns and rfad decreased edn ... | 2010 | 20833130 |
| assessment of the microbiological safety of dried spices and herbs commercialized in spain. | spices and herbs are natural products or their blends that must be free of extraneous matter content. conventional production of these products implicates a number of hygienic problems so spices and herbs may be exposed to a wide range of microbial contamination during pre- and post-harvest and they can present high microbial counts. in this study, we have analyzed the microbial quality of 53 samples of spices and dry herbs collected from spanish markets detecting a contamination of samples of s ... | 2010 | 20848208 |
| burkholderia type vi secretion systems have distinct roles in eukaryotic and bacterial cell interactions. | bacteria that live in the environment have evolved pathways specialized to defend against eukaryotic organisms or other bacteria. in this manuscript, we systematically examined the role of the five type vi secretion systems (t6sss) of burkholderia thailandensis (b. thai) in eukaryotic and bacterial cell interactions. consistent with phylogenetic analyses comparing the distribution of the b. thai t6sss with well-characterized bacterial and eukaryotic cell-targeting t6sss, we found that t6ss-5 pla ... | 2010 | 20865170 |
| influence of microbial interactions and eps/polysaccharide composition on nutrient removal activity in biofilms formed by strains found in wastewater treatment systems. | the study of biofilm function, structure and microbial interactions might help to improve our understanding of biofilm wastewater treatment processes. however, few reports specifically address the influence of interactions within multispecies biofilms on microbial activity and biofilm composition. thus, the relationship between biofilm formation, denitrification activity, phosphorus removal and the composition of extracellular polymeric substances (eps), exopolysaccharides and the bacterial comm ... | 2010 | 20869860 |
| gyrb multiplex pcr to differentiate between acinetobacter calcoaceticus and acinetobacter genomic species 3. | a previously established multiplex pcr that identifies to the species level acinetobacter baumannii and acinetobacter genomic species 13tu (gs13tu) was expanded to include acinetobacter calcoaceticus and acinetobacter genomic species 3. | 2010 | 20881170 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| a beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in cupriavidus necator. | the tcprxabcyd operon of cupriavidus necator jmp134 is involved in the degradation of 2,4,6-trichlorophenol (tcp). all of the gene products except tcpy have assigned functions in tcp metabolism. sequence comparison identified tcpy as a member of cog4313, a group of hypothetical proteins. tcpy has a signal peptide, indicating it is a membrane or secreted protein. secondary structure and topology analysis indicated tcpy as a beta-barrel outer membrane protein, similar to the escherichia coli outer ... | 2010 | 19937267 |
| epidemiology of bloodstream infections in patients with haematological malignancies with and without neutropenia. | all bacterial isolates from 7058 patients admitted to haemato-oncology wards at national taiwan university hospital between 2002 and 2006 were characterized. in total 1307 non-duplicate bloodstream isolates were made from all patients with haematological malignancy; 853 (65%) of these were from neutropenic patients. gram-negative bacteria predominated (60%) in neutropenic isolates with escherichia coli (12%), klebsiella pneumoniae (10%), acinetobacter calcoaceticus-baumannii complex (6%), and st ... | 2010 | 19941686 |
| bacteremia due to acinetobacter genomic species 10. | six patients with acinetobacter genomic species 10 bacteremia were identified. the clinical features of the patients, phenotypic and genotypic identifications, antimicrobial susceptibilities, and genes flanking isaba1 of the bacteria were described. the results revealed that this bacterium is a potentially lethal pathogen that can cause health care-associated infections in debilitated patients. | 2010 | 19955266 |
| systematic review of invasive acinetobacter infections in children. | clinicians are generally familiar with acinetobacter as an etiological agent for serious nosocomial infections in intensive care units. however, there are no previous reviews of the full spectrum of invasive infections in children. | 2010 | 21629616 |
| defective in cuticular ridges (dcr) of arabidopsis thaliana, a gene associated with surface cutin formation, encodes a soluble diacylglycerol acyltransferase. | a key step in the triacylglycerol (tag) biosynthetic pathway is the final acylation of diacylglycerol (dag) by dag acyltransferase. in silico analysis has revealed that the dcr (defective in cuticular ridges) (at5g23940) gene has a typical hx(4)d acyltransferase motif at the n-terminal end and a lipid binding motif vx(2)gf at the middle of the sequence. to understand the biochemical function, the gene was overexpressed in escherichia coli, and the purified recombinant protein was found to acylat ... | 2010 | 20921218 |
| multidrug resistant acinetobacter. | emergence and spread of acinetobacter species, resistant to most of the available antimicrobial agents, is an area of great concern. it is now being frequently associated with healthcare associated infections. literature was searched at pubmed, google scholar, and cochrane library, using the terms 'acinetobacter resistance, multidrug resistant (mdr), antimicrobial therapy, outbreak, colistin, tigecycline, ampc enzymes, and carbapenemases in various combinations. the terms such as mdr, extensivel ... | 2010 | 20927292 |
| probing reactivity of pqq-dependent carbohydrate dehydrogenases using artificial electron acceptor. | the kinetic parameters of carbohydrate oxidation catalyzed by acinetobacter calcoaceticus pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase (gdh) and escherichia coli pqq-dependent aldose sugar dehydrogenase (asdh) were determined using various electron acceptors. the radical cations of organic compounds and 2,6-dichlorophenolindophenol are the most reactive with both enzymes in presence of glucose. the reactivity of dioxygen with asdh is low; the bimolecular constant k (ox) = 660 m ... | 2010 | 20936374 |
| bacterial stimulation of copper phytoaccumulation by bioaugmentation with rhizosphere bacteria. | copper contaminated areas pose environmental health risk to living organisms. remediation processes are thus required for both crop production and industrial activities. this study employed bioaugmentation with copper resistant bacteria to improve phytoremediation of vineyard soils and copper mining waste contaminated with high copper concentrations. oatmeal plant (avena sativa l.) was used for copper phytoextraction. three copper resistant bacterial isolates from oatmeal rhizosphere (pseudomona ... | 2010 | 20937516 |
| effects of water miscible organic solvents on the activity and conformation of the baeyer-villiger monooxygenases from thermobifida fusca and acinetobacter calcoaceticus: a comparative study. | a broader exploitation of enzymes in organic synthesis can be achieved by increasing their tolerance toward organic solvents. in this study, the stability and activity of baeyer-villiger monooxygenases from thermobifida fusca (pamo) and acinetobacter sp. (chmo) in the presence of water miscible organic solvents were compared. pamo was more stable than chmo. the concentration of solvent (v/v) at which it halved its activity (c(50) ) was 4- to 16-fold higher than that observed for chmo. for pamo, ... | 2010 | 20939006 |
| characterization of the exopolysaccharide produced by salipiger mucosus a3, a halophilic species belonging to the alphaproteobacteria, isolated on the spanish mediterranean seaboard. | we have studied the exopolysaccharide produced by the type strain of salipiger mucosus, a species of halophilic, eps-producing (exopolysaccharide-producing) bacterium belonging to the alphaproteobacteria. the strain, isolated on the mediterranean seaboard, produced a polysaccharide, mainly during its exponential growth phase but also to a lesser extent during the stationary phase. culture parameters influenced bacterial growth and eps production. yield was always directly related to the quantity ... | 2010 | 20948906 |
| etiology and antibacterial susceptibility pattern of community-acquired bacterial ocular infections in a tertiary eye care hospital in south india. | to identify the etiology, incidence and prevalence of ocular bacterial infections, and to assess the in vitro susceptibility of these ocular bacterial isolates to commonly used antibiotics. | 2010 | 20952834 |
| microbial contamination of orthodontic buccal tubes from manufacturers. | this study aimed to test the sterility of new unused orthodontic buccal tubes received from manufacturers. four different types of buccal tubes were used straight from the manufactures package without any additional sterilizing step. of these buccal tubes tested, three genera of bacteria, implicated as opportunistic pathogens, namely micrococcus luteus, staphylococcus haemolyticus and acinetobacter calcoaceticus were recovered from these buccal tubes. our data showing microbial contamination on ... | 2010 | 20957099 |
| modulation of pseudomonas aeruginosa biofilm dispersal by a cyclic-di-gmp phosphodiesterase with a putative hypoxia-sensing domain. | pseudomonas aeruginosa encodes many enzymes that are potentially associated with the synthesis or degradation of the widely conserved second messenger cyclic-di-gmp (c-di-gmp). in this study, we show that mutation of rbda, which encodes a fusion protein consisting of pas-pac-ggdef-eal multidomains, results in decreased biofilm dispersal. rbda contains a highly conserved ggdef domain and eal domain, which are involved in the synthesis and degradation of c-di-gmp, respectively. however, in vivo an ... | 2010 | 20971871 |
| increasing fatty acid production in e. coli by simulating the lipid accumulation of oleaginous microorganisms. | unlike many oleaginous microorganisms, e. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. in this study, acetyl-coa carboxylase (acc) from acinetobacter calcoaceticus was expressed in e. coli to redirect the carbon flux to the generation of malonyl-coa, which resulted in a threefold increase in intracellular lipids. moreover, providing a high level of nadph by overexpressing malic enzyme and adding malate to the culture medium resul ... | 2010 | 20972897 |
| use of pcr coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles. | sepsis is among the top 10 causes of mortality in the united states. rapid administration of antibiotics is one of the most important contributors to patient survival, yet only a limited number of methods exist for rapid identification of microbes cultivated from bloodstream infections, which can lead to sepsis. while traditional single-target molecular methods have been shown to greatly improve survival for septic patients by enabling rapid deescalation of broad-spectrum antibiotics, multiplex ... | 2010 | 21048006 |
| use of pcr coupled with electrospray ionization mass spectrometry for rapid identification of bacterial and yeast bloodstream pathogens from blood culture bottles. | sepsis is among the top 10 causes of mortality in the united states. rapid administration of antibiotics is one of the most important contributors to patient survival, yet only a limited number of methods exist for rapid identification of microbes cultivated from bloodstream infections, which can lead to sepsis. while traditional single-target molecular methods have been shown to greatly improve survival for septic patients by enabling rapid deescalation of broad-spectrum antibiotics, multiplex ... | 2010 | 21048006 |
| comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods. | the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ... | 2010 | 21076485 |
| changing carbapenemase gene pattern in an epidemic multidrug-resistant acinetobacter baumannii lineage causing multiple outbreaks in central italy. | infections caused by multidrug-resistant (mdr) acinetobacter baumannii are a challenging problem worldwide. here, the molecular epidemiology and the genetic basis of antibiotic resistance in 111 mdr a. baumannii strains isolated from june 2005 to march 2009 from infected patients in 10 intensive care units (icus) in central italy were investigated. | 2010 | 21088019 |
| changing carbapenemase gene pattern in an epidemic multidrug-resistant acinetobacter baumannii lineage causing multiple outbreaks in central italy. | infections caused by multidrug-resistant (mdr) acinetobacter baumannii are a challenging problem worldwide. here, the molecular epidemiology and the genetic basis of antibiotic resistance in 111 mdr a. baumannii strains isolated from june 2005 to march 2009 from infected patients in 10 intensive care units (icus) in central italy were investigated. | 2010 | 21088019 |
| carbon flux rerouting during mycobacterium tuberculosis growth arrest. | a hallmark of the mycobacterium tuberculosis life cycle is the pathogen's ability to switch between replicative and non-replicative states in response to host immunity. transcriptional profiling by qpcr of ∼ 50 m. tuberculosis genes involved in central and lipid metabolism revealed a re-routing of carbon flow associated with bacterial growth arrest during mouse lung infection. carbon rerouting was marked by a switch from metabolic pathways generating energy and biosynthetic precursors in growing ... | 2010 | 21091505 |
| [changes in the bacterial spectrum in severe burn wounds]. | to determine the time axis for increase in gram negative bacterial strains in burn wounds during hospitalization. | 2010 | 21110445 |
| increasing imipenem resistance and dissemination of the isaba1-associated blaoxa-23 gene among acinetobacter baumannii isolates in an intensive care unit. | the antibiotic susceptibility of acinetobacter calcoaceticus-acinetobacter baumannii complex strains recovered from the intensive care unit (icu) of west china hospital, sichuan, pr china, from 2006 to 2009 was investigated. the identification of a. baumannii and analysis of carbapenemase-encoding genes and their relationship with isaba1 were performed by pcr. furthermore, a diversilab repetitive extragenic palindromic sequence-based pcr (rep-pcr) microbial typing system and a multilocus sequenc ... | 2010 | 21127157 |
| quinone-dependent d-lactate dehydrogenase dld (cg1027) is essential for growth of corynebacterium glutamicum on d-lactate. | corynebacterium glutamicum is able to grow with lactate as sole or combined carbon and energy source. quinone-dependent l-lactate dehydrogenase lldd is known to be essential for utilization of l-lactate by c. glutamicum. d-lactate also serves as sole carbon source for c. glutamicum atcc 13032. | 2010 | 21159175 |
| development of an immunochromatographic strip for simple detection of penicillin-binding protein 2'. | infections with methicillin-resistant staphylococcus aureus (mrsa) and methicillin-resistant coagulase-negative staphylococcus (mr-cns) are a serious problem in hospitals because these bacteria produce penicillin-binding protein 2' (pbp2' or pbp2a), which shows low affinity to β-lactam antibiotics. furthermore, the bacteria show resistance to a variety of antibiotics. identification of these pathogens has been carried out mainly by the oxacillin susceptibility test, which takes several days to p ... | 2010 | 21177917 |
| development of an immunochromatographic strip for simple detection of penicillin-binding protein 2'. | infections with methicillin-resistant staphylococcus aureus (mrsa) and methicillin-resistant coagulase-negative staphylococcus (mr-cns) are a serious problem in hospitals because these bacteria produce penicillin-binding protein 2' (pbp2' or pbp2a), which shows low affinity to β-lactam antibiotics. furthermore, the bacteria show resistance to a variety of antibiotics. identification of these pathogens has been carried out mainly by the oxacillin susceptibility test, which takes several days to p ... | 2010 | 21177917 |
| genes but not genomes reveal bacterial domestication of lactococcus lactis. | the population structure and diversity of lactococcus lactis subsp. lactis, a major industrial bacterium involved in milk fermentation, was determined at both gene and genome level. seventy-six lactococcal isolates of various origins were studied by different genotyping methods and thirty-six strains displaying unique macrorestriction fingerprints were analyzed by a new multilocus sequence typing (mlst) scheme. this gene-based analysis was compared to genomic characteristics determined by pulsed ... | 2010 | 21179431 |
| influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media. | in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ... | 2010 | 21211813 |
| influence of tetracycline resistance on the transport of manure-derived escherichia coli in saturated porous media. | in this research, tetracycline resistant (tet(r)) and tetracycline susceptible (tet(s)) escherichia coli isolates were retrieved from dairy manure and the influence of tetracycline resistance on the transport of e. coli in saturated porous media was investigated through laboratory column transport experiments. experimental results showed that tet(r)e. coli strains had higher mobility than the tet(s) strains in saturated porous media. measurements of cell surface properties suggested that tet(r)e ... | 2010 | 21211813 |
| screening of endophytic bacteria isolated from leaves of sambung nyawa [gynura procumbens (lour.) merr.] for cytokinin-like compounds. | endophytic bacteria are harmless in most plant species; and known to boost the growth and development of the host plants probably by secreting growth hormones. the isolation, identification and screening of endophytic bacteria for the plant growth regulators like cytokinin are needed to get the leads for their applications in agriculture sector. we describe the isolation and identification of the bacterial endophytes from the leaves of sambung nyawa [gynura procumbens (lour.) merr.] and their sc ... | 2010 | 21364796 |
| [peculiarities of ethanol oxidation by the producer of surface-active substances acinetobacter calcoaceticus k-4]. | activity of key-enzymes of c2-metabolism was determined in the cells of strain-producer of surface-active substances acinetobacter calcoaceticus k-4 grown on ethanol. it is shown that ethanol and acetaldehyde oxidation in the strain k-4 is performed by pyroquinolinquinon (pqq) and 4-nitroso-n,n-dimethylaniline (ndma)-dependent dehydrogenases. activity of ndma-dependent enzymes was maximum (100-300 nmol min(-1) mg(-1) of protein) in the early exponential growth phase of a. calcoaceticus k-4. avai ... | 2010 | 21381310 |
| [microbial spectrum of respiratory tract in patients with different pulmonary diseases]. | to study microbial spectrum of respiratory tract in patients with pulmonary diseases. | 2010 | 21384588 |
| hydrophobic substances induce water stress in microbial cells. | ubiquitous noxious hydrophobic substances, such as hydrocarbons, pesticides and diverse industrial chemicals, stress biological systems and thereby affect their ability to mediate biosphere functions like element and energy cycling vital to biosphere health. such chemically diverse compounds may have distinct toxic activities for cellular systems; they may also share a common mechanism of stress induction mediated by their hydrophobicity. we hypothesized that the stressful effects of, and cellul ... | 2010 | 21255365 |
| performance of enhanced biological sbr process for aniline treatment by mycelial pellet as biomass carrier. | mycelial pellet of aspergillus niger y3 was used as a biomass carrier to immobilize the aniline-degrading bacterium, acinetobacter calcoaceticus jh-9 and the mix culture of the cod rapid degradation bacteria. in order to investigate its removal effect on aniline and cod, the combined mycelial pellets were applied in the sbr. comparison of the performances was conducted between another sbr inoculated with sole strain jh-9 and the above sbr. the results showed that the stable degradations of anili ... | 2010 | 21256002 |
| biodegradation of ochratoxin a for food and feed decontamination. | ochratoxin a (ota) is one of the most important mycotoxins that is found in food and feed products. it has proven toxic properties, being primarily known for its nephrotoxicity and carcinogenicity to certain animal species. ota is produced by several species of aspergillus and penicillium that can be found in a wide variety of agricultural products, which makes the presence of ota in these products common. many countries have statutory limits for ota, and concentrations need to be reduced to as ... | 2010 | 22069627 |
| comparison of traditional phenotypic identification methods with partial 5' 16s rrna gene sequencing for species-level identification of nonfermenting gram-negative bacilli. | correct identification of nonfermenting gram-negative bacilli (nfb) is crucial for patient management. we compared phenotypic identifications of 96 clinical nfb isolates with identifications obtained by 5' 16s rrna gene sequencing. sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify nfb. | 2010 | 20164273 |
| skew-laplace and cell-size distribution in microbial axenic cultures: statistical assessment and biological interpretation. | we report a skew-laplace statistical analysis of both flow cytometry scatters and cell size from microbial strains primarily grown in batch cultures, others in chemostat cultures and bacterial aquatic populations. cytometry scatters best fit the skew-laplace distribution while cell size as assessed by an electronic particle analyzer exhibited a moderate fitting. unlike the cultures, the aquatic bacterial communities clearly do not fit to a skew-laplace distribution. due to its versatile nature, ... | 2010 | 20592754 |
| chemical, physical and biological approaches to prevent ochratoxin induced toxicoses in humans and animals. | ochratoxins are polyketide derived fungal secondary metabolites with nephrotoxic, immunosuppressive, teratogenic, and carcinogenic properties. ochratoxin-producing fungi may contaminate agricultural products in the field (preharvest spoilage), during storage (postharvest spoilage), or during processing. ochratoxin contamination of foods and feeds poses a serious health hazard to animals and humans. several strategies have been investigated for lowering the ochratoxin content in agricultural prod ... | 2010 | 22069658 |
| family 6 glycosyltransferases in vertebrates and bacteria: inactivation and horizontal gene transfer may enhance mutualism between vertebrates and bacteria. | glycosyltransferases (gts) control the synthesis and structures of glycans. inactivation and intense allelic variation in members of the gt6 family generate species-specific and individual variations in carbohydrate structures, including histo-blood group oligosaccharides, resulting in anti-glycan antibodies that target glycan-decorated pathogens. gt6 genes are ubiquitous in vertebrates but are otherwise rare, existing in a few bacteria, one protozoan, and cyanophages, suggesting lateral gene tr ... | 2010 | 20870714 |
| antioxidant, antimicrobial activity and toxicity test of pilea microphylla. | a total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from pilea microphylla (urticaceae family) and including toxicity test. antioxidant activity were tested by using dpph free radical scavenging, also total phenolic contents and total flavonoid contents were determined. toxicity assay carried out by using brine shrimps. methanol extract of method i (me i) showed the highest antioxidant activity at 69.51 ... | 2010 | 20652052 |
| rapid method for isolation of desiccation-tolerant strains and xeroprotectants. | a novel biotechnological process has been developed for the isolation of desiccation-tolerant microorganisms and their xeroprotectants, i.e., compatible solutes involved in long-term stability of biomolecules in the dry state. following exposure of soil samples to chloroform, we isolated a collection of desiccation-tolerant microorganisms. this collection was screened for the production of xeroprotectants by a variation of the bacterial milking (osmotic downshock) procedure and by a novel air-dr ... | 2010 | 20562279 |
| in vitro activity of tigecycline against patient isolates collected during phase 3 clinical trials for hospital acquired pneumonia. | the in vitro activity of tigecycline was evaluated against 819 baseline pathogens isolated from 383 patients enrolled in the phase 3 clinical trial investigating the efficacy of tigecycline in hospital acquired pneumonia (hap). the trials were global, enrolling patients in 27 countries. tigecycline was active against the most prevalent pathogens in hap, including gram-positive and gram-negative strains (90% of mics ≤2 µg/ml for the entire collection). the spectrum of activity of tigecycline incl ... | 2010 | 24470884 |
| fungal biodegradation and enzymatic modification of lignin. | lignin, the most abundant aromatic biopolymer on earth, is extremely recalcitrant to degradation. by linking to both hemicellulose and cellulose, it creates a barrier to any solutions or enzymes and prevents the penetration of lignocellulolytic enzymes into the interior lignocellulosic structure. some basidiomycetes white-rot fungi are able to degrade lignin efficiently using a combination of extracellular ligninolytic enzymes, organic acids, mediators and accessory enzymes. this review describe ... | 2010 | 21968746 |
| ochratoxin a in ruminants−a review on its degradation by gut microbes and effects on animals. | ruminants are much less sensitive to ochratoxin a (ota) than non-ruminants. the ruminal microbes, with protozoa being a central group, degrade the mycotoxin extensively, with disappearance half lives of 0.6-3.8 h. however, in some studies ota was detected systemically when using sensitive analytical methods, probably due to some rumen bypass at proportions of estimated 2-6.5% of dosage (maximum 10%). high concentrate proportions and high feeding levels are dietary factors promoting the likelines ... | 2010 | 22069612 |
| concomitant production of protease and lipase by bacillus licheniformis vsg1: production, purification and characterization. | this study was aimed at producing protease and lipase simultaneously on a common medium by bacillus licheniformis vsg1, which was isolated from a tannery effluent. the effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. both those enzymes were produced under optimized conditions like ph, temperature and incubation time. the enzyme mixture comprising of both protease and lipase was purified by ammonium sulp ... | 2010 | 24031479 |
| recommended design features of future clinical trials of antibacterial agents for hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. | 2010 | 20597666 | |
| pharmacokinetics and safety of single and multiple doses of achn-490 injection administered intravenously in healthy subjects. | achn-490 is an aminoglycoside with activity against multidrug-resistant pathogens, including those resistant to currently used aminoglycosides. two randomized, double-blind, placebo-controlled clinical studies investigated the pharmacokinetics (pk), safety, and tolerability of achn-490 injection in healthy subjects. study 1 used a parallel-group design with escalating single (sd) and multiple doses (md). study 2 explored a longer duration of the highest dose tolerated in the first study. subject ... | 2011 | 21911572 |
| advances in the management of sepsis and the understanding of key immunologic defects. | anesthesiologists are increasingly confronting the difficult problem of caring for patients with sepsis in the operating room and in the intensive care unit. sepsis occurs in more than 750,000 patients in the united states annually and is responsible for more than 210,000 deaths. approximately 40% of all intensive care unit patients have sepsis on admission to the intensive care unit or experience sepsis during their stay in the intensive care unit. there have been significant advances in the un ... | 2011 | 21952252 |
| the role of synthetic biology in the design of microbial cell factories for biofuel production. | insecurity in the supply of fossil fuels, volatile fuel prices, and major concerns regarding climate change have sparked renewed interest in the production of fuels from renewable resources. because of this, the use of biodiesel has grown dramatically during the last few years and is expected to increase even further in the future. biodiesel production through the use of microbial systems has marked a turning point in the field of biofuels since it is emerging as an attractive alternative to con ... | 2011 | 22028591 |
| evolutionary view of acyl-coa diacylglycerol acyltransferase (dgat), a key enzyme in neutral lipid biosynthesis. | triacylglycerides (tags) are a class of neutral lipids that represent the most important storage form of energy for eukaryotic cells. dgat (acyl-coa: diacylglycerol acyltransferase; ec 2.3.1.20) is a transmembrane enzyme that acts in the final and committed step of tag synthesis, and it has been proposed to be the rate-limiting enzyme in plant storage lipid accumulation. in fact, two different enzymes identified in several eukaryotic species, dgat1 and dgat2, are the main enzymes responsible for ... | 2011 | 21933415 |
| functional characterization of a ficolin-mediated complement pathway in amphioxus. | the ficolin-mediated complement pathway plays an important role in vertebrate immunity, but it is not clear whether this pathway exists in invertebrates. here we identified homologs of ficolin pathway components from the cephalochordate amphioxus and investigated whether they had been co-opted into a functional ficolin pathway. four of these homologs, ficolin fcn1, serine protease masp1 and masp3, and complement component c3, were highly expressed in mucosal tissues and gonads, and were signific ... | 2011 | 21832079 |
| infection-associated clinical outcomes in hospitalized medical evacuees after traumatic injury: trauma infectious disease outcome study. | 2011 | 21795875 |