Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| nkg2d ligand mica is retained in the cis-golgi apparatus by human cytomegalovirus protein ul142. | human cytomegalovirus (hcmv) evades t-cell recognition by down-regulating expression of major histocompatibility complex (mhc) class i and ii molecules on the surfaces of infected cells. contrary to the "missing-self" hypothesis, hcmv-infected cells are refractory to lysis by natural killer (nk) cells. inhibition of nk cell function is mediated by a number of hcmv immune evasion molecules, which operate by delivering inhibitory signals to nk cells and preventing engagement of activating ligands. ... | 2009 | 19793804 |
| human cytomegalovirus interacts with toll-like receptor 2 and cd14 on syncytiotrophoblasts to stimulate expression of tnfalpha mrna and apoptosis. | placentae from newborns with congenital human cytomegalovirus (hcmv) infection often display shallow implantation, chronic villitis and disruptions of the syncytiotrophoblast. little is known about how hcmv infection induces inflammation in the placenta and loss of the trophoblast. we propose that the inflammation is initiated with innate defense responses of mature syncytiotrophoblast (st) to virus. previously we have shown that ultraviolet irradiation-inactivated (uv-) hcmv interacts with toll ... | 2009 | 19796811 |
| detection of viral dna in kidney graft preservation and washing solutions is predictive of posttransplant infections in pediatric recipients. | in pediatric kidney transplant recipients, viral infections occur soon after transplant and may be transmitted from the graft. | 2009 | 19803803 |
| hexamethylene bisacetamide can convert nonpermissive human cells to a permissive state for expressing the major immediate-early genes of human cytomegalovirus by up-regulating nf-kappab activity. | expression of the major immediate-early (mie) genes of human cytomegalovirus (hcmv) in the human thyroid papillary carcinoma cell line tpc-1 is repressed at the transcriptional level. however, treatment of these cells with hexamethylene bisacetamide (hmba), a chemical inducer of differentiation, for 12 to 24 h before infection enabled the cells to support ie1 and ie2 gene expression and consequently hcmv replication. in hmba-treated cells the transcription factor nf-kappab was induced and the mi ... | 2009 | 19027925 |
| high-level expression of glycoprotein d by a dominant-negative hsv-1 virus augments its efficacy as a vaccine against hsv-1 infection. | using the t-rex (invitrogen, carlsbad, ca) gene switch technology, we previously generated a dominant-negative herpes simplex virus (hsv)-1 recombinant, cj83193, capable of inhibiting its own replication as well as that of wild-type hsv-1 and hsv-2. it has been further demonstrated that cj83193 is an effective vaccine against hsv-1 infection in a mouse ocular model. to ensure its safety and augment its efficacy, we generated an improved cj83193-like hsv-1 recombinant, cj9-gd, which contains a de ... | 2009 | 19005489 |
| high cytomegalovirus load and prolonged virus excretion in breast milk increase risk for viral acquisition by very low birth weight infants. | breast milk is the main source of postnatal human cytomegalovirus (hcmv) infection. the aim of this study was to assess the relationship between hcmv load in breast milk and viral transmission to very low birth weight (vlbw) infants. | 2009 | 19687768 |
| human cytomegalovirus productively infects adrenocortical cells and induces an early cortisol response. | following our recent findings on the presence of human cytomegalovirus (hcmv) in the normal human adrenal cortex and in adrenocortical tumors, especially in cortisol-secreting tumors, aim of the present study was to investigate the direct effects of hcmv infection on human adrenocortical cells. to this aim, both clinical isolates and laboratory strains of hcmv were used to assess the early effects of infection on human adrenocortical cell morphology, proliferation, gene expression, and steroidog ... | 2009 | 19688782 |
| identification of hla-a*2402-restricted hcmv immediate early-1 (ie-1) epitopes as targets for cd8+ hcmv-specific cytotoxic t lymphocytes. | to identify novel hla-a*2402-restricted human cytomegalovirus (hcmv) immediate early-1 (ie-1) epitopes for adoptive immunotherapy, we explored 120 overlapping 15-amino acid spanning ie-1. | 2009 | 19698161 |
| human cytomegalovirus in peripheral giant cell granuloma. | the peripheral giant cell granuloma is a relatively common non-neoplastic inflammatory lesion of gingiva, but the etiopathogeny remains unknown. this study aimed to evaluate the importance of human cytomegalovirus and epstein-barr virus in a peripheral giant cell granuloma of a 47-year-old female. | 2009 | 19702955 |
| single and multiple mutations in the human cytomegalovirus ul97 gene and their relationship to the enzymatic activity of ul97 kinase for ganciclovir phosphorylation. | in this study we determined that the double mutant m460v/d605e in the ul97 gene of an hcmv isolate from an immunocompromised patient (mmt isolate) is related to resistance to ganciclovir (gcv) therapy. our results suggest that the aspartic acid-to-glutamic acid substitution at codon 605 may be associated with a natural polymorphism of the ul97 gene, and not with positive selection pressure exerted by the antiviral drug. we also determined that gcv resistance due to the m460v mutation in the hcmv ... | 2009 | 19712701 |
| the trc8 e3 ligase ubiquitinates mhc class i molecules before dislocation from the er. | the us2 and us11 gene products of human cytomegalovirus promote viral evasion by hijacking the endoplasmic reticulum (er)-associated degradation (erad) pathway. us2 and us11 initiate dislocation of newly translocated major histocompatibility complex class i (mhc i) from the er to the cytosol for proteasome-mediated degradation, thereby decreasing cell surface mhc i. despite being instrumental in elucidating the mammalian erad pathway, the responsible e3 ligase or ligases remain unknown. using a ... | 2009 | 19720873 |
| real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis. | we described the proof-of-principle of a nonoptical real-time pcr that uses cyclic voltammetry for indirectly monitoring the amplified dna product generated in the pcr reaction solution after each pcr cycle. to enable indirect measurement of the amplicon produced throughout pcr, we monitor electrochemically the progressive consumption (i.e., the decrease of concentration) of free electroactive deoxynucleoside triphosphates (dntps) used for dna synthesis. this is accomplished by exploiting the fa ... | 2009 | 19722651 |
| inhibition of human cytomegalovirus replication via peptide aptamers directed against the nonconventional nuclear localization signal of the essential viral replication factor pul84. | the ul84 open reading frame of human cytomegalovirus encodes an essential multifunctional regulatory protein that is thought to act in the nucleus as an initiator of lytic viral replication. nuclear trafficking of pul84 is facilitated by a complex nonconventional nuclear localization signal (nls) that mediates its interaction with the cellular importin-alpha/beta pathway. since binding of pul84 to importin-alpha proteins mechanistically differs from that of cellular proteins containing a classic ... | 2009 | 19740994 |
| conserved retinoblastoma protein-binding motif in human cytomegalovirus ul97 kinase minimally impacts viral replication but affects susceptibility to maribavir. | the ul97 kinase has been shown to phosphorylate and inactivate the retinoblastoma protein (rb) and has three consensus rb-binding motifs that might contribute to this activity. recombinant viruses containing mutations in the rb-binding motifs generally replicated well in human foreskin fibroblasts with only a slight delay in replication kinetics. their susceptibility to the specific ul97 kinase inhibitor, maribavir, was also examined. mutation of the amino terminal motif, which is involved in th ... | 2009 | 19159461 |
| identification of trim23 as a cofactor involved in the regulation of nf-kappab by human cytomegalovirus. | human cytomegalovirus (hcmv) regulates nf-kappab during infection by a variety of mechanisms. for example, the hcmv gene product, ul144, is known to activate nf-kappab in a tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6)-dependent manner, causing the upregulation of the chemokine ccl22 (mdc). viral ul144 is expressed from the ul/b' region of the hcmv genome at early times postinfection and is a tnfr1-like homologue. despite this homology to the tnfr1 receptor superfamily, ul144 ... | 2009 | 19176615 |
| cyclin-dependent kinases phosphorylate the cytomegalovirus rna export protein pul69 and modulate its nuclear localization and activity. | replication of human cytomegalovirus (hcmv) is subject to regulation by cellular protein kinases. recently, we and others reported that inhibition of cyclin-dependent protein kinases (cdks) or the viral cdk ortholog pul97 can induce intranuclear speckled aggregation of the viral mrna export factor, pul69. here we provide the first evidence for a direct regulatory role of cdks on pul69 functionality. although replication of all hcmv strains was dependent on cdk activity, we found strain-specific ... | 2009 | 19179338 |
| rapid determination of antiviral drug susceptibility of human cytomegalovirus by real-time pcr. | a quantitative real-time pcr-based assay was developed for determination of cytomegalovirus (hcmv) susceptibility to antiviral drugs. after hcmv isolate-growth for 4 days, antiviral drug susceptibility was determined by measuring the reduction of intracellular hcmv dna in the presence of increasing concentrations of either ganciclovir, or foscarnet or cidofovir. the 50% inhibitory concentration (ic(50)) was the drug concentration that reduced the number of hcmv genome copies by 50%. the ic(50) v ... | 2009 | 18992772 |
| genetic vaccines for anthrax based on recombinant adeno-associated virus vectors. | bacillus anthracis represents a formidable bioterrorism and biowarfare threat for which new vaccines are needed with improved safety and efficacy over current options. toward this end, we created recombinant adeno-associated virus type 1 (raav1) vectors containing synthetic genes derived from the protective antigen (pa) or lethal factor (lf) of anthrax lethal toxin (letx) and tested them for immunogenicity and induction of toxin-neutralizing antibodies in rabbits. codon-optimized segments encodi ... | 2009 | 19002162 |
| next generation tools for high-throughput promoter and expression analysis employing single-copy knock-ins at the hprt1 locus. | we have engineered a set of useful tools that facilitate targeted single copy knock-in (ki) at the hypoxanthine guanine phosphoribosyl transferase 1 (hprt1) locus. we employed fine scale mapping to delineate the precise breakpoint location at the hprt1(b-m3) locus allowing allele specific pcr assays to be established. our suite of tools contains four targeting expression vectors and a complementing series of embryonic stem cell lines. two of these vectors encode enhanced green fluorescent protei ... | 2009 | 18950699 |
| herpesviridae and novel inhibitors. | herpesviridae comprises a large family of double-stranded dna viruses that infect both animals and humans. eight herpesviruses are known to infect humans: herpes simplex virus type-1 and -2, varicella zoster virus, human cytomegalovirus, epstein-barr virus, human herpesvirus 6 type-a and -b, human herpesvirus type-7 and -8 or kaposi's sarcoma virus. despite the fact that the past two decades have been evolutionary in the development of antiviral agents, therapeutic choices are restricted by limi ... | 2009 | 20032535 |
| association of human t lymphotropic virus 1 amplification of periodontitis severity with altered cytokine expression in response to a standard periodontopathogen infection. | periodontal diseases (pds) are infectious diseases in which periodontopathogens trigger chronic inflammatory and immune responses that lead to tissue destruction. recently, viruses have been implicated in the pathogenesis of pds. individuals infected with human t lymphotropic virus 1 (htlv-1) present with abnormal oral health and a marked increased prevalence of periodontal disease. | 2010 | 20038241 |
| high prevalence of human cytomegalovirus in a population of periodontally healthy subjects. | human cytomegalovirus (hcmv) appears to be more frequent in periodontally affected patients than in healthy control groups. based on this assumption, it has been suggested that hcmv may play a role in the pathogenesis of periodontal disease. | 2010 | 20038920 |
| activation of peroxisome proliferator-activated receptor gamma by human cytomegalovirus for de novo replication impairs migration and invasiveness of cytotrophoblasts from early placentas. | human cytomegalovirus (hcmv) contributes to pathogenic processes in immunosuppressed individuals, in fetuses, and in neonates. in the present report, by using reporter gene activation assays and confocal microscopy in the presence of a specific antagonist, we show for the first time that hcmv infection induces peroxisome proliferator-activated receptor gamma (ppargamma) transcriptional activity in infected cells. we demonstrate that the ppargamma antagonist dramatically impairs virus production ... | 2010 | 20042507 |
| proteasome subunits relocalize during human cytomegalovirus infection, and proteasome activity is necessary for efficient viral gene transcription. | we have continued studies to further understand the role of the ubiquitin-proteasome system (ups) in human cytomegalovirus (hcmv) infection. with specific inhibitors of the proteasome, we show that ongoing proteasome activity is necessary for facilitating the various stages of the infection. immediate-early protein 2 expression is modestly reduced with addition of proteasome inhibitors at the onset of infection; however, both early and late gene expression are significantly delayed, even if the ... | 2010 | 20042513 |
| the inhibitor of cyclin-dependent kinases, olomoucine ii, exhibits potent antiviral properties. | olomoucine ii, the most recent derivative of roscovitine, is an exceptionally potent pharmacological inhibitor of cyclin-dependent kinase activities. here, we report that olomoucine ii is also an effective antiviral agent. | 2010 | 20054100 |
| human cytomegalovirus reinfection is associated with intrauterine transmission in a highly cytomegalovirus-immune maternal population. | to determine contribution of reinfection with new strains of cytomegalovirus in cytomegalovirus seromimmune women to incidence of congenital cytomegalovirus infection. | 2010 | 20060091 |
| human cytomegalovirus tropism for endothelial/epithelial cells: scientific background and clinical implications. | human cytomegalovirus (hcmv) has been routinely isolated from and propagated in vitro in human embryonic lung fibroblast (helf) cell cultures, while in vivo it is known to infect predominantly endothelial and epithelial cells. in recent years, genetic determinants of the hcmv tropism for endothelial/epithelial cells were identified in the ul131a/ul130/ul128 locus of hcmv genome of wild-type strains. ul131a-ul128 gene products form a complex with glycoprotein h (gh) and l (gl) resulting in a gh/g ... | 2010 | 20084641 |
| ex vivo monitoring of human cytomegalovirus-specific cd8(+) t-cell responses using the quantiferon-cmv assay in allogeneic hematopoietic stem cell transplant recipients attending an irish hospital. | reconstitution of human cytomegalovirus (hcmv) t-cell immunity is crucial in hematopoietic stem cell transplant (hsct) recipients. the quantiferon-cmv assay for cellular hcmv-specific immunity was evaluated in allogeneic hsct recipients (n = 43) and patients with hematological malignancies (n = 29) attending a tertiary-care irish hospital. an intracellular cytokine (icc) assay correlated with the quantiferon-cmv assay. although there was agreement between hcmv seropositivity and quantiferon-cmv ... | 2010 | 20087937 |
| bicaudal d1-dependent trafficking of human cytomegalovirus tegument protein pp150 in virus-infected cells. | human cytomegalovirus (hcmv) virion assembly takes place in the nucleus and cytoplasm of infected cells. the hcmv virion tegument protein pp150 (ppul32) is an essential protein of hcmv and has been suggested to play a role in the cytoplasmic phase of hcmv assembly. to further define its role in viral assembly and to identify host cell proteins that interact with pp150 during viral assembly, we utilized yeast two-hybrid analyses to detect an interaction between pp150 and bicaudal d1 (bicd1), a pr ... | 2010 | 20089649 |
| antiviral and anti-inflammatory metabolites from the soft coral sinularia capillosa. | chemical investigations of the soft coral sinularia capillosa resulted in the isolation of one new tetraprenylbenzoquinone, capilloquinone (1), two new furanobenzosesquiterpenoids, capillobenzopyranol (2) and capillobenzofuranol (3), one new furanosesquiterpenoid, capillofuranocarboxylate (4), and five previously characterized metabolites, comprising (e)-5-(2,6-dimethylocta-5,7-dienyl)furan-3-carboxylic acid (5), 2-[(2e,6e)-3,7-dimethyl-8-(4-methylfuran-2-yl)octa-2,6-dienyl]-5-methylcyclohexa-2, ... | 2010 | 20155971 |
| [proteomic study of the brain tissues of mice with human cytomegalovirus infection]. | to establish the two-dimensional electrophoresis profiles with high resolution and reproducibility from the brain tissues of mice with human cytomegalovirus (hcmv) infection. | 2010 | 20159717 |
| regulation of the subcellular distribution of key cellular rna-processing factors during permissive human cytomegalovirus infection. | alternative splicing and polyadenylation of human cytomegalovirus (hcmv) immediate-early (ie) pre-mrnas are temporally regulated and rely on cellular rna-processing factors. this study examined the location and abundance of essential rna-processing factors, which affect alternative processing of ul37 ie pre-mrnas, during hcmv infection. serine/threonine protein kinase 1 (srpk1) phosphorylates serine/arginine-rich proteins, necessary for pre-spliceosome commitment. it was found that hcmv infectio ... | 2010 | 20164265 |
| protective immunity transferred by infusion of cytomegalovirus-specific cd8(+) t cells within donor grafts: its associations with cytomegalovirus reactivation following unmanipulated allogeneic hematopoietic stem cell transplantation. | human cytomegalovirus (cmv)-specific cytotoxic t lymphocyte (ctl) immune response must be reconstituted for long-term protection against cmv relapse and disease in hematopoietic stem cell transplantation (hsct) recipients. we phenotypically quantitated absolute numbers of cmv-pp65 peptide-specific ctls (ctl(cmv)) in 50 related donor unmanipulated allografts infused into hla-matched or -mismatched recipients and examined the incidence of cmv reactivation. high ctl(cmv) with terminally differentia ... | 2010 | 20167279 |
| apo b100 similarities to viral proteins suggest basis for ldl-dna binding and transfection capacity. | ldl mediates transfection with plasmid dna in a variety of cell types in vitro and in several tissues in vivo in the rat. the transfection capacity of ldl is based on apo b100, as arginine/lysine clusters, suggestive of nucleic acid-binding domains and nuclear localization signal sequences, are present throughout the molecule. apo e may also contribute to this capacity because of its similarity to the dengue virus capsid proteins and its ability to bind dna. synthetic peptides representing two a ... | 2010 | 20173184 |
| point mutations in bhv-1 us3 gene abolish its ability to induce cytoskeletal changes in various cell types. | the us3 gene is conserved among alphaherpesviruses and codes for a protein kinase, a multifunctional protein involved in many phases of virus infection, like nuclear egress, modulation of apoptosis and modification of the cellular cytoskeleton. bovine herpesvirus (bhv-1), a member of the alphaherpesvirinae, contains an open reading frame homologous to us3 of other herpesviruses, which has been identified as a serine/threonine kinase (takashima, y., tamura, h., xuan, x., otsuka, h., 1999. identif ... | 2010 | 20197221 |
| novel mode of phosphorylation-triggered reorganization of the nuclear lamina during nuclear egress of human cytomegalovirus. | the nucleocytoplasmic egress of viral capsids is a rate-limiting step in the replication of the human cytomegalovirus (hcmv). as reported recently, an hcmv-specific nuclear egress complex is composed of viral and cellular proteins, in particular protein kinases with the capacity to induce destabilization of the nuclear lamina. viral protein kinase pul97 and cellular protein kinase c (pkc) play important roles by phosphorylating several types of nuclear lamins. using pul97 mutants, we show that t ... | 2010 | 20202933 |
| fusion of the human cytomegalovirus pp65 antigen with both ubiquitin and ornithine decarboxylase additively enhances antigen presentation to cd8(+) t cells in human dendritic cells. | antigenic molecules are modified for targeting to the proteasome by ubiquitin (ub) or by a ub-independent system such as ornithine decarboxylase (odc) to be presented by mhc class i molecules. in this study, we compared the immunogenicity of human cytomegalovirus pp65 antigen fused with ub and/or odc, using rna electroporation of human dendritic cells. among the c-terminal mutants of ub (g76, a76, and v76), ub(g) showed the best ability to enhance the degradation of a target protein and stimulat ... | 2010 | 20218861 |
| dendritic cell vaccination in an allogeneic stem cell recipient receiving a transplant from a human cytomegalovirus (hcmv)-seronegative donor: induction of a hcmv-specific t(helper) cell response. | in the absence of a protective immune response, human cytomegalovirus (hcmv) infection remains a life-threatening complication after allogeneic stem cell transplantation (sct), especially in recipients of grafts from hcmv-seronegative donors. after allogeneic sct from a seronegative donor, prolonged and severe immune deficiency often leads to infectious complications. vaccination with antigen-loaded dendritic cells (dc) has been shown to be a potent approach for the induction of antigen-specific ... | 2010 | 20230226 |
| cytomegalovirus vaccines and methods of production (wo20009049138): the emerging recognition of the importance of virus neutralization at the epithelial/endothelial interface. | human cytomegalovirus (cmv) is the most common cause of congenital viral infection in the developed world. approximately 40,000 congenitally infected infants are born in the us each year. congenital cmv infection is responsible for a wide range of neurodevelopmental disabilities and is the most common infectious cause of hearing loss in children. the significant public health impact of congenital cmv has led the institute of medicine to rank development of a cmv vaccine as a top priority. vaccin ... | 2010 | 20302454 |
| antitumor-promoting activity of lignans: inhibition of human cytomegalovirus ie gene expression. | chemoprevention is a promising new approach to cancer prevention. since the beginning of chemoprevention studies, short-term in vitro models used in the study of carcinogenesis have been applied in the identification of antitumor-promoting agents. | 2010 | 20332453 |
| [phage display selection on mrc-5 cells yield peptides specific for hcmv-binding]. | to screen the special binding peptides to the mrc-5 cells infected by human cytomegalovirus (hcmv) so as to study the progress how hcmv recognizes susceptive host cells. | 2010 | 20369459 |
| saliva as a source of hcmv dna in allogeneic stem cell transplantation patients. | the purpose of this study was to investigate the use of saliva for the identification of human cytomegalovirus (hcmv) in allogeneic hematopoietic stem cell transplant patients by real time pcr compared with blood. | 2010 | 20374507 |
| intravitreal ganciclovir in the management of non-aids-related human cytomegalovirus retinitis. | to report and evaluate intravitreal ganciclovir injections in non-aids patients with human cytomegalovirus (hcmv) retinitis. | 2010 | 20379302 |
| inhibition of nkg2d expression in nk cells by cytokines secreted in response to human cytomegalovirus infection. | the nkg2d receptor activates natural killer (nk) cell cytotoxicity and cytokine production on recognition of self-molecules induced by cellular stress under different conditions such as viral infections. the importance of nkg2d in the immune response to human cytomegalovirus (hcmv) is supported by the identification of several viral molecules that prevent the expression of nkg2d ligands by infected cells. in this study we report that, paradoxically, a significant, selective, and transient reduct ... | 2010 | 20393128 |
| mir-132 regulates antiviral innate immunity through suppression of the p300 transcriptional co-activator. | micrornas are small, non-coding rnas that negatively regulate gene expression. it has been proposed that micrornas could function in the regulation of innate immunity, but this has not been demonstrated for viral infection. here we test this hypothesis using the human pathogenic virus kaposi's sarcoma-associated herpesvirus (kshv) and one of its putative natural cellular targets, primary lymphatic endothelial cells (lecs). we show that an early antiviral microrna response (6 h post-infection) in ... | 2010 | 20418869 |
| [screening and identification of cd13-binding peptides with phage display peptide library]. | to screen and identify the peptides that specifically bind to cd13 on monocytes. | 2010 | 20423858 |
| bioactive norditerpenoids from the soft coral sinularia gyrosa. | chemical investigations of the soft coral sinularia gyrosa resulted in the isolation of six new norcembranolides, gyrosanolides a-f (1-6), a new norcembrane, gyrosanin a (7), and 11 known norditerpenoids 8-18. the structures of the isolated compounds were elucidated through extensive spectroscopic data and by comparison with reported data in the literature. compounds 1-3, 7-9, 12, and 13 at concentration of 10microm did not inhibit the cox-2 protein expression, but significantly reduced the leve ... | 2010 | 20430633 |
| immune responses in pigs induced by recombinant canine adenovirus 2 expressing the glycoprotein 5 of porcine reproductive and respiratory syndrome virus. | to develop a new type vaccine for porcine reproductive and respiratory syndrome (prrs) prevention by using canine adenovirus 2(cav-2) as vector, the glycoprotein 5(gp5) gene from prrsv strain jl was amplified by rt-pcr, and the expression cassette of gp5 was constructed using the human cytomegalovirus (hcmv) promoter and the simian virus 40 (sv40) early mrna polyadenylation signal. the expression cassette of glycoprotein 5 was cloned into the cav-2 genome in which e3 region had been partly delet ... | 2010 | 20432066 |
| optimal replication of human cytomegalovirus correlates with endocytosis of glycoprotein gpul132. | envelopment of a herpesvirus particle is a complex process of which much is still to be learned. we previously identified the glycoprotein gpul132 of human cytomegalovirus (hcmv) as an envelope component of the virion. in its carboxy-terminal portion, gpul132 contains at least four motifs for sorting of transmembrane proteins to endosomes; among them are one dileucine-based signal and three tyrosine-based signals of the yxxø and npxy (where x stands for any amino acid, and ø stands for any bulky ... | 2010 | 20444903 |
| positive predictive value of anti-hcmv igm as an index of primary infection. | a search for specific igm antibodies was used for the detection of primary human cytomegalovirus (hcmv) infections, but the significance of the results is limited by the possible persistence of specific igm over time, the fact that they are also produced during episodes of reactivation or reinfection, and possible cross-reactions with other viruses. anti-hcmv antibody screening was carried out to assess the positive predictive value of detecting specific igm antibodies using igg and igm enzyme-l ... | 2010 | 20470827 |
| sequential mutations associated with adaptation of human cytomegalovirus to growth in cell culture. | mutations that occurred during adaptation of human cytomegalovirus to cell culture were monitored by isolating four strains from clinical samples, passaging them in various cell types and sequencing ten complete virus genomes from the final passages. mutational dynamics were assessed by targeted sequencing of intermediate passages and the original clinical samples. gene rl13 and the ul128 locus (ul128l, consisting of genes ul128, ul130 and ul131a) mutated in all strains. mutations in rl13 occurr ... | 2010 | 20479471 |
| analysis of dna repeats in bacterial plasmids reveals the potential for recurrent instability events. | structural instability has been frequently observed in natural plasmids and vectors used for protein expression or dna vaccine development. however, there is a lack of information concerning hotspot mapping, namely, dna repeats or sequences identical to the host genome. this led us to evaluate the abundance and distribution of direct, inverted, and tandem repeats with high recombination potential in 36 natural plasmids from ten bacterial genera, as well as in several widely used bacterial and ma ... | 2010 | 20496146 |
| short hairpin rnas specific to human cytomegalovirus terminase subunit pul89 prevent viral maturation. | in order to define the role of the human cytomegalovirus (hcmv) small terminase subunit pul89, analysis by rna interference was applied. | 2010 | 20516558 |
| role of noncovalent sumo binding by the human cytomegalovirus ie2 transactivator in lytic growth. | the 86-kda immediate-early 2 (ie2) protein of human cytomegalovirus (hcmv) is a promiscuous transactivator essential for viral gene expression. ie2 is covalently modified by sumo at two lysine residues (k175 and k180) and also interacts noncovalently with sumo. although sumoylation of ie2 has been shown to enhance its transactivation activity, the role of sumo binding is not clear. here we showed that sumo binding by ie2 is necessary for its efficient transactivation function and for viral growt ... | 2010 | 20519406 |
| asp2151, a novel helicase-primase inhibitor, possesses antiviral activity against varicella-zoster virus and herpes simplex virus types 1 and 2. | to evaluate and describe the anti-herpesvirus effect of asp2151, amenamevir, a novel non-nucleoside oxadiazolylphenyl-containing herpesvirus helicase-primase complex inhibitor. | 2010 | 20534624 |
| role of the specific interaction of ul112-113 p84 with ul44 dna polymerase processivity factor in promoting dna replication of human cytomegalovirus. | the human cytomegalovirus (hcmv) ul112-113 region encodes four phosphoproteins with common amino termini (p34, p43, p50, and p84) via alternative splicing and is thought to be required for efficient viral dna replication. we have previously shown that interactions among the four ul112-113 proteins regulate their intranuclear targeting and enable the recruitment of the ul44 dna polymerase processivity factor to viral prereplication foci. here, we show that in virus-infected cells, the ul112-113 p ... | 2010 | 20538862 |
| human cytomegalovirus ie86 protein binds to cellular mcm3 protein but does not inhibit its binding to the epstein-barr virus orip in u373mg-p220.2 cells. | human cytomegalovirus (hcmv) or its immediate-early ie86 protein alone induces cell cycle in quiescent primary human foreskin fibroblasts (hffs), but blocks its progression at the g1/s interphase and inhibits cellular dna synthesis by a mechanism that is not clearly understood. it is assumed that, in this phenomenon, the binding of minichromosome maintenance (mcm) proteins to replication origins is blocked. in this work, we analyzed the initiation of dna replication in hcmv-permissive u373mg cel ... | 2010 | 20545442 |
| alteration of lipid metabolism in cells infected with human cytomegalovirus. | the human cytomegalovirus (hcmv) envelope contains 12 virus-encoded glycoproteins and glycoprotein complexes but the lipid composition of the envelope has not been clearly defined. given the specificity of the interactions between integral membrane proteins and lipids, it is likely that the lipid content of the virion envelope is regulated during infection. in an effort to determine the effects of hcmv infection on lipid metabolism, we have used pcr array technology to investigate how infection ... | 2010 | 20552728 |
| comparative evaluation of eight commercial human cytomegalovirus igg avidity assays. | the interpretation of a positive igm antibody result to human cytomegalovirus (hcmv) in a pregnant woman is of major importance for the correct management of the pregnancy. determination of hcmv-specific igg avidity is considered an useful approach for distinguishing igm antibody due to primary hcmv infection from igm antibody elicited during non-primary infection. | 2010 | 20561816 |
| human cytomegalovirus ul144 is associated with viremia and infant development sequelae in congenital infection. | human cytomegalovirus (hcmv) strains may be genotyped based on polymorphisms that exist within the ul144 gene, which is one of 19 viral genes lost in attenuated laboratory strains. in the present study, ul144 genotypes in congenitally infected babies (congenital cytomegalovirus [ccmv]) were determined, and the relationship between the genotype, viral load, cytokine profile, and patient developmental outcome was investigated. all ccmv infections identified during 2006 and 2007 were included (n = ... | 2010 | 20810771 |
| detection of human cytomegalovirus in bronchoalveolar lavage fluid of lung transplant recipients reflects local virus replication and not contamination from the throat. | whether bronchoalveolar lavage (bal) fluid may be contaminated with oropharyngeal cytomegalovirus (cmv) has never been investigated. in an analysis of cmv dna loads in 76 simultaneously obtained bal fluid and throat wash samples from lung transplant recipients, we show that such contamination is unlikely and that detection of cmv dna in bal fluid reflects virus replication in the lung. | 2010 | 20810778 |
| transcription pattern of ul131a-128 mrna in clinical strains of human cytomegalovirus. | human cytomegalovirus (hcmv) mrna was obtained from human embryonic lung fibroblast cells infected by hcmv clinical strains from urine samples of infants at different kinetic periods. the cdna of ul131a-128 mrnas was amplified using reverse transcription-polymerase chain reaction (rt-pcr) and analysed by sequencing. meanwhile, clones containing ul131a-128 transcripts in an hcmv cdna library of a clinical strain were selected and sequenced. it was demonstrated that ul131a-128 mrna was expressed w ... | 2010 | 20826945 |
| cyclin-dependent kinase activity controls the onset of the hcmv lytic cycle. | the onset of human cytomegalovirus (hcmv) lytic infection is strictly synchronized with the host cell cycle. infected g0/g1 cells support viral immediate early (ie) gene expression and proceed to the g1/s boundary where they finally arrest. in contrast, s/g2 cells can be infected but effectively block ie gene expression and this inhibition is not relieved until host cells have divided and reentered g1. during latent infection ie gene expression is also inhibited, and for reactivation to occur th ... | 2010 | 20844576 |
| antiviral drug resistance of human cytomegalovirus. | the study of human cytomegalovirus (hcmv) antiviral drug resistance has enhanced knowledge of the virological targets and the mechanisms of antiviral activity. the currently approved drugs, ganciclovir (gcv), foscarnet (fos), and cidofovir (cdv), target the viral dna polymerase. gcv anabolism also requires phosphorylation by the virus-encoded ul97 kinase. gcv resistance mutations have been identified in both genes, while fos and cdv mutations occur only in the dna polymerase gene. confirmation o ... | 2010 | 20930070 |
| identification of binary interactions between human cytomegalovirus virion proteins. | human cytomegalovirus (hcmv) virions are composed of a dna-containing nucleocapsid surrounded by a tegument layer and host-derived lipid envelope studded with virally encoded glycoproteins. these complex virions are estimated to be composed of more than 50 viral proteins. assembly of hcmv virions is poorly understood, especially with respect to acquisition of the tegument; however, it is thought to involve the stepwise addition of virion components through protein-protein interactions. we sought ... | 2010 | 20962080 |
| oncomodulation by human cytomegalovirus: novel clinical findings open new roads. | the question whether human cytomegalovirus may affect cancer diseases has been discussed (very controversially) for decades. there are convinced believers and strict opponents of the idea that hcmv might be able to play a role in the course of cancer diseases. in parallel, the number of published reports on the topic is growing. recently published and presented (ranganathan p, clark p, kuo js, salamat s, kalejta rf. a survey of human cytomegalovirus genomic loci present in glioblastoma multiform ... | 2010 | 20967552 |
| 2-azetidinone--a new profile of various pharmacological activities. | 2-azetidinone, a β-lactam four member heterocyclic compound involved in research aimed to evaluate new products that possess interesting biological activities. these compounds reported for their antimicrobial and antifungal activities. successful introduction of aztreonam as a potent inhibitor of cephalosporinase and ezetimibe as a cholesterol absorption inhibitor proved potential of 2-azetidinone moiety. subsequently 2-azetidinones were highlighted as a potent mechanism based inhibitor of sever ... | 2010 | 20970895 |
| [be vigilant of human cytomegalovirus infection in the diagnosis and treatment of ulcerative colitis]. | 2010 | 20979780 | |
| experimental human cytomegalovirus latency in cd14+ monocytes. | cd14(+) monocytes are a reservoir for latent human cytomegalovirus, and virus replication is reactivated during their differentiation to macrophages or dendritic cells. it has not been clear whether the virus can establish latency upon direct infection of monocytes or whether it must first become quiescent in a progenitor cell that subsequently differentiates to generate a monocyte. we report that infection of primary human monocytes with a clinical strain of human cytomegalovirus exhibits the h ... | 2010 | 21041645 |
| human cytomegalovirus early protein pul21a promotes efficient viral dna synthesis and the late accumulation of immediate-early transcripts. | we have previously reported that a newly annotated gene of human cytomegalovirus (hcmv), ul21a, encodes an early viral protein termed pul21a. most notably, the virions of a ul21a deletion virus had markedly reduced infectivity, indicating that ul21a is required to establish an efficient productive infection. in this study, we infected fibroblasts with equal numbers of dna-containing viral particles and identified where in the viral life cycle pul21a acted. the ul21a deletion virus entered cells ... | 2010 | 21047969 |
| alternative splicing of the human cytomegalovirus major immediate-early genes affects infectious-virus replication and control of cellular cyclin-dependent kinase. | the major immediate-early (mie) gene locus of human cytomegalovirus (hcmv) is the master switch that determines the outcomes of both lytic and latent infections. here, we provide evidence that alteration in the splicing of hcmv (towne strain) mie genes affects infectious-virus replication, movement through the cell cycle, and cyclin-dependent kinase activity. mutation of a conserved 24-nucleotide region in mie exon 4 increased the abundance of ie1-p38 mrna and decreased the abundance of ie1-p72 ... | 2010 | 21068259 |
| diversity of antiviral-resistant human cytomegalovirus in heart and lung transplant recipients. | immunocompromised transplant recipients are at high risk for human cytomegalovirus (cmv)-related infection and disease. antiviral prophylaxis and treatment have reduced cmv morbidity and mortality, but at times promote development of antiviral-resistant cmv strains that can significantly contribute to adverse clinical outcomes in transplant recipients. we have investigated cmv genotypes in transplant recipients (bone marrow, stem cell, kidney, heart, lung, and liver) receiving antiviral prophyla ... | 2010 | 21070537 |
| prevention of maternal cytomegalovirus infection: current status and future prospects. | human cytomegalovirus (cmv) infection is the most common cause of perinatal viral infection in the developed world, resulting in approximately 40,000 congenitally infected infants in the united states each year. congenital cmv infection can produce varying degrees of neurodevelopmental disabilities. the significant impact of congenital cmv has led the institute of medicine to rank development of a cmv vaccine as a top priority. vaccine development has been ongoing; however no licensed cmv vaccin ... | 2010 | 21072294 |
| human cytomegalovirus induces tgf-β1 activation in renal tubular epithelial cells after epithelial-to-mesenchymal transition. | human cytomegalovirus (hcmv) infection is associated epidemiologically with poor outcome of renal allografts due to mechanisms which remain largely undefined. transforming growth factor-β1 (tgf-β1), a potent fibrogenic cytokine, is more abundant in rejecting renal allografts that are infected with either hcmv or rat cmv as compared to uninfected, rejecting grafts. tgf-β1 induces renal fibrosis via epithelial-to-mesenchymal transition (emt) of renal epithelial cells, a process by which epithelial ... | 2010 | 21079788 |
| inhibition of calmodulin-dependent kinase kinase blocks human cytomegalovirus-induced glycolytic activation and severely attenuates production of viral progeny. | viruses depend on the host cell to provide the energy and biomolecular subunits necessary for production of viral progeny. we have previously reported that human cytomegalovirus (hcmv) infection induces dramatic changes to central carbon metabolism, including glycolysis, the tricarboxylic acid (tca) cycle, fatty acid biosynthesis, and nucleotide biosynthesis. here, we explore the mechanisms involved in hcmv-mediated glycolytic activation. we find that hcmv virion binding and tegument protein del ... | 2010 | 21084482 |
| guinea pig cytomegalovirus gp84 is a functional homolog of the human cytomegalovirus (hcmv) ul84 gene that can complement for the loss of ul84 in a chimeric hcmv. | the guinea pig cytomegalovirus (gpcmv) co-linear gene and potential functional homolog of hcmv ul84 (gp84) was investigated. the gp84 gene had delayed early transcription kinetics and transient expression studies of gp84 protein (pgp84) demonstrated that it targeted the nucleus and co-localized with the viral dna polymerase accessory protein as described for hcmv pul84. additionally, pgp84 exhibited a transdominant inhibitory effect on viral growth as described for hcmv. the inhibitory domain co ... | 2010 | 21094510 |
| [effect of periodontal mechanical treatment on herpesviruses in gingival crevicular fluid of patients with chronic periodontitis]. | to evaluate the subgingival prevalence of human cytomegalovirus (hcmv), epstein-barr virus-1 (ebv-1) in chronic periodontitis (cp) patients before and after treatment and to analyze the relationship between the prevalent variance and periodontal clinical parameters. | 2010 | 21122436 |
| a case of epstein-barr virus associated post-transplant lymphoproliferative disorder with cns involvement: pathological findings at both biopsy and autopsy. | post-transplant lymphoproliferative disorder (ptld) with cns involvement is an uncommon and fatal side effect of immunosuppressants. a 55-year-old man presented with non-fluent aphasia, fever, neck stiffness and disturbance of consciousness. twenty-one years previously, the patient had undergone kidney transplantation for chronic renal failure. brain mri revealed multiple lesions in the bilateral cerebrum, right cerebellum and medulla oblongata. the brain biopsy showed ebv-positive lymphocytes i ... | 2010 | 21134001 |
| characterization of the betaherpesviral pul69 protein family reveals binding of the cellular mrna export factor uap56 as a prerequisite for stimulation of nuclear mrna export and for efficient viral replication. | ul69 of human cytomegalovirus (hcmv) encodes a pleiotropic transactivator protein and has a counterpart in every member of the herpesviridae family thus far sequenced. however, little is known about the conservation of the functions of the nuclear phosphoprotein pul69 in the homologous proteins of other betaherpesviruses. therefore, eukaryotic expression vectors were constructed for pc69 of chimpanzee cytomegalovirus, prh69 of rhesus cytomegalovirus, pm69 of murine cytomegalovirus, pu42 of human ... | 2010 | 21147923 |
| natural killer cell receptor expression reflects the role of human cytomegalovirus in the pathogenesis of a subset of cd4+ t-cell large granular lymphocytosis. | a high frequency of cd4(+) t-cell large granular lymphocyte (t-lgl) lymphocytosis occurs in human leukocyte antigen (hla) -drb1*0701 individuals displaying monoclonal expansions of vβ13.1+ cd4(+) t-cell clones, which specifically respond to human cytomegalovirus (hcmv) antigens. we previously reported the expression of natural killer (nk)-cell associated receptors (nkr) by hcmv-specific cytolytic cd4(+) t cells from healthy donors. in the present study a high expression of different nkr (i.e., n ... | 2010 | 21156193 |
| in vivo evaluation of a biodegradable donut-shaped minitablet for prolonged posterior segment drug delivery in the rabbit eye model. | this study focused on the in vivo evaluation of a biodegradable ganciclovir-loaded donut-shaped minitablet (dsmt) for controlled drug delivery in the new zealand white albino rabbit eye model. specialized tablet tooling was used to manufacture a poly(lactic-co-glycolic acid) dsmt device that was implanted into 18 rabbits through the pars plana/peripheral retina of the right eyes of each rabbit. the left eyes were used as controls. possible adverse effects on ocular tissues were assessed by histo ... | 2010 | 21165921 |
| inhibition of swine leukocyte antigen-i presentation in transgenic mini-pig cell lines by expressing human cytomegalovirus us6. | xenotransplantation using porcine organs may resolve the chronic shortage of donor organs for clinical transplantation if significant immunologic barriers can be overcome. a xenograft can be rejected by t cells, especially cd8(+) cytotoxic t lymphocytes (ctl)-mediated responses, as these cells show cytotoxicity against xenografts by recognition of swine leukocyte antigen (sla)-i. peptide translocation is inhibited by the endoplasmic reticulum-resident human cytomegalovirus (hcmv) glycoprotein un ... | 2010 | 21168753 |
| narrowing of t-cell receptor beta variable repertoire during symptomatic herpesvirus infection in transplant patients. | primary infection or recrudescence of latent virus infection in transplant recipients can be manifested either as asymptomatic or symptomatic disease. here we show that symptomatic human cytomegalovirus (hcmv) or epstein-barr virus (ebv) infection or recrudescence following solid organ transplantation (sot) was coincident with a dramatic skewing of t-cell receptor beta variable (trbv) repertoire, with expansions of monoclonal/oligoclonal clonotypes. as the clinical symptoms resolved, the periphe ... | 2010 | 19806163 |
| survivin expression in odontogenic keratocysts and correlation with cytomegalovirus infection. | the aim of this study was to investigate the expression of survivin, an inhibitor of apoptosis, in odontogenic keratocysts and to compare it to the findings in non-neoplastic jaw cysts - periapical cysts, as well as to establish a possible relationship between survivin expression and human cytomegalovirus presence within these cysts. | 2010 | 19659890 |
| molecular detection of cytomegalovirus, herpes simplex virus 2, human papillomavirus 16-18 in turkish pregnants. | human cytomegalovirus (cmv) is the most common cause of viral intrauterine infections in the world. herpes simplex virus type 2 (hsv-2) and human papillomavirus (hpv) are the main agents of viral sexually transmitted diseases, which cause genital ulcers and genital warts, respectively. hpv infection has been linked to the majority of the anogenital malignancies. the aim of this study was to detect the existence of cmv, hsv-2 and hpv type 16-18 in turkish pregnants by using sensitive molecular as ... | 2010 | 21340297 |
| differential resistance to antiviral drugs in an immunocompromised patient with cytomegalovirus encephalitis. | 2010 | 20801077 | |
| [incidence and relationship of human cytomegalovirus infection and human herpesvirus 6 infection in pediatric patients after hemopoietic stem cell transplantation]. | to study the incidence of human cytomegalovirus (cmv) and human herpesvirus 6 (hhv-6) infection in pediatric patients with hemopoietic stem cell transplantation (hsct), and to explore the relationship between cmv and hhv-6 infection in pediatric patients with hsct. | 2010 | 21604575 |
| a new tool linking human cytomegalovirus drug resistance mutations to resistance phenotypes. | drug resistant strains of human cytomegalovirus (hcmv) in patients at risk may increasingly develop into a problem in the clinical setting. genotypic resistance testing is becoming the method of choice, but requires previous phenotypic characterisation of each newly found mutation. in order to facilitate the interpretation of the patient's cmv sequence data, a web-based search tool was generated that links the sequence to a database containing all published ul97 (protein kinase) and ul54 (dna po ... | 2010 | 19853628 |
| [evaluation of the lightcycler 480 real-time pcr system for the measurement of cmv, ebv, hhv-6 and bkv viral loads in whole blood]. | the roche lightcycler 480 (lc480) system was evaluated for quantitative molecular diagnosis of opportunistic viral infections caused by human cytomegalovirus (cmv), epstein-barr virus (ebv), human herpesvirus 6 (hhv-6), and bk virus (bkv), in comparison with "in-house" real-time pcr assays. | 2010 | 19892483 |
| sequences of complete human cytomegalovirus genomes from infected cell cultures and clinical specimens. | we have assessed two approaches to sequencing complete human cytomegalovirus (hcmv) genomes (236 kbp) in dna extracted from infected cell cultures (strains 3157, han13, han20 and han38) or clinical specimens (strains jp and 3301). the first approach involved amplifying genomes from the dna samples as overlapping pcr products, sequencing these by the sanger method, acquiring reads from a capillary instrument and assembling these using the staden programs. the second approach involved generating s ... | 2010 | 19906940 |
| analysis of latent viral gene expression in natural and experimental latency models of human cytomegalovirus and its correlation with histone modifications at a latent promoter. | human cytomegalovirus (hcmv) is an opportunistic human pathogen that establishes a lifelong latent infection, which can reactivate periodically. if unchecked by a robust immune response, this reactivation can result in severe disease in immunocompromised patients. reactivation of latent virus in myeloid progenitor cells is concomitant with cellular differentiation through regulation of the major immediate-early promoter (miep) by chromatin remodelling. in this study, we analysed the expression o ... | 2010 | 19906945 |
| successful immunotherapy of hcmv disease using virus-specific t cells expanded from an allogeneic stem cell transplant recipient. | opportunistic infection remains the principal cause of mortality in allogeneic stem cell transplant recipients with active extensive chronic graft-versus-host disease. human cytomegalovirus (hcmv) represents an important cause of disease in this setting and the toxicity of protracted and recurrent antiviral treatment together with eventual drug resistance represents a significant limitation to therapy. although the expansion and adoptive transfer of hcmv-specific t cells from the healthy origina ... | 2010 | 19919660 |
| sequence flexibility of the immunodominant hla a*0201 restricted ppul83 cd8 t-cell epitope of human cytomegalovirus. | the cytomegalovirus ppul83 protein contains an immunodominant a*0201 restricted epitope between residues 495 and 503. we investigated the tolerance of this epitope to sequence variation in the context of peptide binding to hla a*0201 and the ability to induce an interferon gamma (ifngamma) response through engagement with the t-cell receptor (tcr). the majority of mutations investigated resulted in a decrease in the production of ifngamma indicating that if such variants occurred in vivo they wo ... | 2010 | 19950243 |
| the varicella-zoster virus ie4 protein: a conserved member of the herpesviral mrna export factors family and a potential alternative target in antiherpetic therapies. | during a viral infection, in addition to cellular mrnas, amounts of viral mrnas have to be efficiently transported to the cytoplasm for translation. it is now established that herpesviruses encode a conserved gene family whose proteins act as viral mrna export factors that mediate nucleocytoplasmic transport of viral transcripts and eventually modulate through this mechanism the antiviral response. this conserved family of proteins contains the ie4 protein of the varicella-zoster virus (vzv). he ... | 2010 | 20650265 |
| optimized recombinant dense bodies of human cytomegalovirus efficiently prime virus specific lymphocytes and neutralizing antibodies without the addition of adjuvant. | control of human cytomegalovirus (hcmv) infection correlates with the reconstitution of antiviral t lymphocytes in haematopoietic stem cell transplant recipients. a vaccine to foster this reconstitution and to ameliorate the severe consequences of hcmv reactivation is yet unavailable. this work focused on providing a rationale for the amendment of the yields and the antigenic composition of a vaccine, based on subviral dense bodies (db) of hcmv. modified db were generated that contained the hla- ... | 2010 | 20655401 |
| the glycoprotein b disintegrin-like domain binds beta 1 integrin to mediate cytomegalovirus entry. | cellular integrins were identified as human cytomegalovirus (hcmv) entry receptors and signaling mediators in both fibroblasts and endothelial cells. the goal of these studies was to determine the mechanism by which hcmv binds to cellular integrins to mediate virus entry. hcmv envelope glycoprotein b (gb) has sequence similarity to the integrin-binding disintegrin-like domain found in the adam (a disintegrin and metalloprotease) family of proteins. to test the ability of this region to bind to c ... | 2010 | 20660204 |
| human cytomegalovirus (hcmv) replication kinetics in stem cell transplant recipients following anti-hcmv therapy. | cytomegalovirus (hcmv) remains an important infection following stem cell transplantation (sct) and is managed via pre-emptive therapy. in some patients hcmv loads continue to increase after therapy and they experience multiple episodes of replication. | 2010 | 20667769 |
| cd45ra expression on hcmv-specific effector memory cd8+ t cells is associated with the duration and intensity of hcmv replication after transplantation. | in this cross-sectional study on 42 solid organ transplant recipients, the association of kinetics of human cytomegalovirus (hcmv) replication and emra hcmv-specific cd8+ t cells was investigated. correlation was observed between the duration of hcmv replication after transplantation and cd45ra+cd27- (r=0.609; p=0.004), cd45ra+cd28- (r=0.579; p=0.008) or cd45ra+ccr7- (r=0.488; p=0.029) hcmv-specific cd8+ t cells percentages. in the multivariate regression analyses, cd45ra+cd27-, cd45ra+cd28- or ... | 2010 | 20674505 |
| hcmv-encoded chemokine receptor us28 mediates proliferative signaling through the il-6-stat3 axis. | us28 is a viral g protein (heterotrimeric guanosine triphosphate-binding protein)-coupled receptor encoded by the human cytomegalovirus (hcmv). in addition to binding and internalizing chemokines, us28 constitutively activates signaling pathways linked to cell proliferation. here, we show increased concentrations of vascular endothelial growth factor and interleukin-6 (il-6) in supernatants of us28-expressing nih 3t3 cells. increased il-6 was associated with increased activation of the signal tr ... | 2010 | 20682912 |
| nuclear localization of tegument-delivered pp71 in human cytomegalovirus-infected cells is facilitated by one or more factors present in terminally differentiated fibroblasts. | herpesviral virions contain a tegument layer that consists primarily of viral proteins. the delivery of fully functional proteins to infected cells upon virion envelope fusion to the plasma membrane allows herpesviruses to modulate cellular activities prior to viral gene expression. certain tegument proteins can also regulate viral processes. for example, the pp71 tegument protein encoded by the ul82 gene of human cytomegalovirus (hcmv) stimulates viral immediate early (ie) gene expression and t ... | 2010 | 20686028 |