Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| phylogenetic background of attaching and effacing escherichia coli isolates from animals. | detection and distribution of eae gene in forty-four attaching and effacing escherichia coli (aeec) strains of animal origin were investigated. association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. phylogenetic analysis showed that 31 ehec/eae+ stec strains belong to groups a, b1 and e, 13 epec strains segregated in b1 and b2. moreover, group a possessed the eae gamma2/theta type, group b1 the eae beta1, eae kapp ... | 2008 | 18509740 |
| a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants. | the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ... | 2008 | 18513960 |
| dietary arginine supplementation alleviates intestinal mucosal disruption induced by escherichia coli lipopolysaccharide in weaned pigs. | this study evaluated whether arginine (arg) supplementation could attenuate gut injury induced by escherichia coli lipopolysaccharide (lps) challenge through an anti-inflammatory role in weaned pigs. pigs were allotted to four treatments including: (1) non-challenged control; (2) lps-challenged control; (3) lps+0.5 % arg; (4) lps+1.0 % arg. on day 16, pigs were injected with lps or sterile saline. at 6 h post-injection, pigs were killed for evaluation of small intestinal morphology and intestina ... | 2008 | 18275628 |
| escherichia coli stb toxin binding to sulfatide and its inhibition by carragenan. | escherichia coli heat-stb is an important cause of diarrhea in piglets. stb was shown to interact specifically with sulfatide (3'-sulfogalactosyl-ceramide) present on the surface of epithelial cells of piglet jejunum. basic data are lacking on stb binding to sulfatide in solution and more precisely on the possible inhibition of this interaction. using surface plasmon resonance technology, we compare binding of stb to sulfatide and other glycoshingolipids previously shown, with a multiplate-bindi ... | 2008 | 18279334 |
| indirect transmission of escherichia coli o157:h7 occurs readily among swine but not among sheep. | transmission of escherichia coli o157:h7 among reservoir animals is generally thought to occur either by direct contact between a naïve animal and an infected animal or by consumption of food or water containing the organism. although ruminants are considered the major reservoir, there are two reports of human infections caused by e. coli o157:h7 linked to the consumption of pork products or to the contamination of fresh produce by swine manure. the objective of this study was to determine wheth ... | 2008 | 18310419 |
| a homolog of the o157 urease-encoding o island 48 is present in porcine o149:h10 enterotoxigenic escherichia coli. | the relationship of the urease operon in the highly virulent o149 porcine enterotoxigenic escherichia coli (etec) strain ro8 to a genomic island (gi) homologous to o island (oi) 48 of o157 enterohemorrhagic e. coli (ehec) strain edl933 was investigated. eighty-four of 84 o149:h10 strains were urease positive whereas 44 of 44 o149:h43 porcine etec strains were urease-negative. seventeen of 17 o149:h10 strains that were tested possessed the oi-48 homolog whereas 24 of 24 o149:h43 strains lacked th ... | 2008 | 18316019 |
| antibiotic resistance among indicator bacteria isolated from healthy pigs in new zealand. | to determine the resistance to antibiotics among the indictor bacteria, escherichia coli and enterococcus spp, isolated from the faeces of healthy pigs on three conventional pig farms and one organic farm in the north island of new zealand. | 2008 | 18322557 |
| salmonella spp. in bavarian liquid pig manure: occurrence and relevance for the distribution of antibiotic resistance. | in a representative study, 380 manure samples of pig farms distributed all over bavaria (germany) were screened for the presence of salmonella spp. at the time of manure application to soil using methods adapted to din en iso 6579. the isolates were tested according to din 58940 for their susceptibility towards 26 antimicrobial substances - such substances partly administered in animals, but mainly used in human therapy. six out of 380 manure samples and, in addition, the only separated liquid m ... | 2008 | 18331515 |
| functional role of polyhydroxy compounds on protein structure and thermal stability studied by circular dichroism spectroscopy. | polyhydroxy compounds such as cyclitols, acyclic polyols and sugars are produced by a wide variety of organisms under stressful conditions in order to protect macromolecular structure. plants undergoing abiotic stresses like heat and dehydration accumulate enormous amounts of polyhydroxy compounds (up to 400 mm) in their cellular tissues. not only do they serve as osmoprotectants ("compatible solutes"), they also protect membrane structure and preserve enzymatic activity. to gain further insight ... | 2008 | 18343146 |
| molecular cloning and characterization of a bile salt hydrolase from lactobacillus acidophilus pf01. | phenotypic screening for bile salt hydrolase (bsh) activity was performed on lactobacillus acidophilus pf01 isolated from piglet feces. a gene encoding bsh was identified and cloned from the genomic library of l. acidophilus pf01. the bsh gene and surrounding regions were characterized by nucleotide sequence analysis and were found to contain a single open reading frame (orf) of 951 nucleotides encoding a 316 amino acid protein. the potential bsh promoter region was located upstream of the start ... | 2008 | 18388461 |
| inhibition of escherichia coli o157:h7 in ripening dry fermented sausage by ground yellow mustard. | compounds generated by the enzymatic hydrolysis of glucosinolates naturally present in mustard powder are potently bactericidal against escherichia coli o157:h7. because e. coli o157:h7 can survive the dry fermented sausage manufacturing process, 2, 4, and 6% (wt/wt) nondeheated (hot) mustard powder or 6% (wt/wt) deheated (cold) mustard powder were added to dry sausage batter inoculated with e. coli o157:h7 at about 7 log cfu/g to evaluate the antimicrobial effectiveness of the powders. reductio ... | 2008 | 18389690 |
| espfu, a type iii-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by e. coli o157:h7. | enterohaemorrhagic escherichia coli (ehec) o157:h7 induces filamentous actin-rich 'pedestals' on intestinal epithelial cells. pedestal formation in vitro requires translocation of bacterial effectors into the host cell, including tir, an ehec receptor, and espf(u), which increases the efficiency of actin assembly initiated by tir. while inactivation of espf(u) does not alter colonization in two reservoir hosts, we utilized two disease models to explore the significance of espf(u)-promoted actin ... | 2008 | 18067584 |
| effects of direct haemoperfusion through fibres immobilizing polymyxin b and nafamostat mesilate on endotoxaemia in conscious guinea-pigs. | 1. direct haemoperfusion through a network of fibres immobilizing polymyxin b (pmx-b) is used for the treatment of septic shock, but the mechanism underlying its clinical benefits remains unclear. the aims of the present study were to assess the actions of direct haemoperfusion through fibres immobilizing pmx-b (pmx-dhp) on the effects of exogenously administered endotoxin in conscious guinea-pigs and to examine the difference in the effects of heparin compared with nafamostat mesilate (nm) used ... | 2008 | 18047622 |
| the consequences of non-starch polysaccharide solubility and inclusion level on the health and performance of weaned pigs challenged with enterotoxigenic escherichia coli. | the inclusion of insoluble nsp (insp) in weaner pig diets has been reported to decrease post-weaning colibacillosis (pwc). conversely, soluble nsp (snsp) have been shown to exacerbate pwc. the present study investigated the effect of nsp solubility and inclusion level on the health and performance of newly weaned pigs challenged with enterotoxigenic escherichia coli (etec), using nsp sources known not to affect digesta viscosity, in a 2 x 2 x 2 factorial combination of nsp solubility (insp v. sn ... | 2008 | 17761008 |
| effect of antimicrobial dosage regimen on salmonella and escherichia coli isolates from feeder swine. | a body of evidence exists that suggests that antimicrobial use in food animals leads to resistance in both pathogenic and commensal bacteria. this study focused on the impact of three different antimicrobial regimes (low-level continuous, pulse, and no antimicrobial) for two antimicrobials (chlortetracycline and tylosin) on the presence of salmonella spp. and on the prevalence of antimicrobial resistance of both salmonella spp. and nonspecific escherichia coli in fecal samples from feeder swine. ... | 2008 | 18223115 |
| a survey of the microbiological quality of feral pig carcasses processed for human consumption in queensland, australia. | an investigation of the microbiological quality of 217 feral pig carcasses at a queensland wild game processing plant was carried out using the excision method of sampling. samples were analyzed for aerobic plate count (apc), e. coli, and salmonella over a period of 2 years. the mean adjusted apc was 4.7 log colony-forming units (cfu)/g, and counts at the 90th, 95th, and 99th percentiles were 4.8, 5.2, and 6.1 log cfu/g, respectively. the maximum number of bacteria recovered was 6.4 log cfu/g, a ... | 2008 | 18260821 |
| investigation of bioaerosols released from swine farms using conventional and alternative waste treatment and management technologies. | microbial air pollution from concentrated animal feeding operations (cafos) has raised concerns about potential public health and environmental impacts. we investigated the levels of bioaerosols released from two swine farms using conventional lagoon-sprayfield technology and ten farms using alternative waste treatment and management technologies in the united states. in total, 424 microbial air samples taken at the 12 cafos were analyzed for several indicator and pathogenic microorganisms, incl ... | 2008 | 19192808 |
| comparison of swabbing and destructive methods for microbiological pig carcass sampling. | to compare the belgian swabbing sampling method for pig carcasses with the reference destructive method with regard to escherichia coli and aerobic plate counts, salmonella and campylobacter prevalence and their relationship. | 2008 | 19241527 |
| prevalence of tir gene subtypes in escherichia coli isolates from pigs. | 2008 | 18836158 | |
| [cloning and expression of the pig skeletal muscle musclin gene]. | we found seven tag sequence with high homology in dbest by using human musclin gene, and got its cdna sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by rt-pcr, encoding 132 amino acid residue protein. the new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pgex-4t-1, and the recombinant was transformed into e. coli bl21. induced by iptg, the fusion ... | 2008 | 18837403 |
| determination of the mimic epitope of the m-like protein adhesin in swine streptococcus equi subsp. zooepidemicus. | the m-like protein, also known as szp, is expressed on the surface of streptococcus equi subsp. zooepidemicus (s. zooepidemicus). previous studies demonstrated that szp is similar to m protein of group a streptococcus in the structure and characteristics of antiphagocytosis. the m protein is an adhesin that can bind to the host cells, however it is not known whether the szp of s. zooepidemicus also functions as an adhesin. we conducted an investigation to determine szp as an adhesin, and one szp ... | 2008 | 18840263 |
| examination of salmonella and escherichia coli translocation from hog manure to forage, soil, and cattle grazed on the hog manure-treated pasture. | use of hog (sus scrofa) manure as a fertilizer is a practical solution for waste re-utilization, however, it may serve as a vehicle for environmental and domestic animal contamination. work was conducted to determine whether pathogens, naturally present in hog manure could be detected in cattle (bos taurus) grazed on the manure-treated pasture, and whether forage contamination occurred. during two 3 mo summer trials manure was applied to yield < or = 124 kg available n per hectare in a single sp ... | 2008 | 18948461 |
| purification, molecular cloning, heterologous expression and characterization of pig cyp1a2. | porcine cytochrome p450 (cyp) 1a2 was purified to electrophoretic homogeneity from the hepatic microsomes of beta-naphthoflavone-treated male pigs. in a reconstituted system, this enzyme showed a good catalytic activity towards caffeine, acetanilide, and methoxyresorufin, all known markers of mammalian cyp1a2. using 3'- and 5'-rapid amplification of coding dna (cdna) ends (race), we amplified from the liver rna of control pigs a full-length 1827 bp cdna containing an open reading frame of 1548 b ... | 2008 | 18949657 |
| genetic variation at the alpha-1-fucosyltransferase (fut1) gene in asian wild boar and chinese and western commercial pig breeds. | escherichia coli f18 bacteria producing enterotoxins and/or shigatoxin (etec/stec) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha-1-fucosyltransferase (fut1) gene has been identified as a candidate gene for controlling the expression of etec f18 receptor. the genetic variations at nucleotide position 307 in open reading frame of fut1 gene in one wild boar breed and 20 western commercial and chinese native pig breeds were investigated by polymerase ch ... | 2008 | 19134079 |
| escherichia coli stb toxin and colibacillosis: knowing is half the battle. | expression of both adherence and enterotoxin expression are required for enterotoxigenic escherichia coli (etec) strains to cause colibacillosis. etec strains are responsible for diarrhea in humans and animals by production of various enterotoxins. for many years, the role of the heat-stable e. coli enterotoxin stb as a diarrhea-causing toxin in animals, and in particular in swine, has been controversial. in fact, although the presence of stb-positive e. coli strains and diarrhea in animals is f ... | 2008 | 17995951 |
| expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect elisa. | porcine circovirus type 2 (pcv2) is considered to be associated with post-weaning multisystemic wasting syndrome (pmws), which is a newly emerged economically important swine disease. the entire coding region of open reading frame 2 (orf2), encoding the viral capsid protein (cap), of pcv2 was cloned and sequenced from the clinical specimen obtained from pmws-affected piglets. six recombinant subunits, a-f, spanning the defined regions of cap were produced by e. coli expression system and used as ... | 2008 | 17996970 |
| time- and dose-related regional fluxes of tissue-type plasminogen activator in anesthetized endotoxemic pigs. | acute endotoxinemia elicits an early fibrinolytic response. this study analyzes the effects of the dose and duration of endotoxin infusion on arterial levels of tissue-type plasminogen activator (tpa) and pulmonary, mesenteric and hepatic plasma tpa fluxes. | 2008 | 17999715 |
| molecular characterization of a novel type of prostamide/prostaglandin f synthase, belonging to the thioredoxin-like superfamily. | prostaglandin f (pgf) ethanolamide (prostamide f) synthase, which catalyzed the reduction of prostamide h(2) to prostamide f(2alpha), was found in mouse and swine brain. the enzyme was purified from swine brain, and its amino acid sequence was defined. the mouse enzyme consisted of a 603-bp open reading frame coding for a 201-amino acid polypeptide with a molecular weight of 21,669. the amino acid sequence placed the enzyme in the thioredoxin-like superfamily with cys(44) being the active site. ... | 2008 | 18006499 |
| the effect of intramuscular administration of colistin on the development and course of experimentally induced oedema disease in weaned piglets. | shiga-toxigenic e. coli (stec) strains that produce shiga toxin stx2e cause oedema disease in weaned piglets. the purpose of the present study was to investigate the impact of stx2e released in mesenteric lymph nodes on disease pathogenesis. colistin and ampicillin were intramuscularly administered to piglets of the experimental group simultaneously challenged with stec strain, type o139:f18ab, stx2e+. piglets of the control group were challenged with stec only. the strain was naturally resistan ... | 2008 | 17977672 |
| molecular cloning and functional characterization of a histidine decarboxylase from staphylococcus capitis. | histamine intoxication is probably the best known toxicological problem of food-borne disease. a histamine-producing staphylococcus capitis strain has been isolated from a cured meat product. the aim of this study was to gain deeper insights into the genetic determinants for histamine production in staph. capitis. | 2008 | 17887985 |
| productivity improvement of recombinant escherichia coli fermentation via robust optimization. | a nonlinear model of a recombinant escherichia coli producing porcine growth hormone (pgh) fermentation was developed. the model was used to calculate a glucose feeding and temperature strategy to optimize the production of pgh. simulations showed that the implementation of optimal feed and temperature profiles was sensitive to the maximum specific growth rate, and a mismatch could result in excessive acetate production and a significant reduction in pgh yield. an optimization algorithm was thus ... | 2008 | 17717709 |
| development and validation of a recombinant capsid protein-based elisa for detection of antibody to porcine circovirus type 2. | porcine circovirus type 2 (pcv2) has been recently associated with a number of disease syndromes, especially postweaning multisystemic wasting disease (pmws). herein, an alternative indirect enzyme-linked immunosorbent assay (elisa) for detection of pcv2 antibody was developed using nuclear localization signal-truncated capsid protein of pcv2 produced in escherichia coli (cap elisa). this assay was validated by comparison with an indirect immunofluorescence assay (iif) and a pcv2-based elisa. th ... | 2008 | 17467754 |
| farm-level risk factors for fluoroquinolone resistance in e. coli and thermophilic campylobacter spp. on finisher pig farms. | logistic regression, supported by other statistical analyses was used to explore the possible association of risk factors with the fluoroquinolone (fq)-resistance status of 108 pig finisher farms in great britain. the farms were classified as 'affected' or 'not affected' by fq-resistant e. coli or campylobacter spp. on the basis of isolation of organisms from faecal samples on media containing 1 mg/l fq. the use of fq was the most important factor associated with finding resistant e. coli and/or ... | 2009 | 19134238 |
| defatted bovine colostrum-supplemented diet around weaning improves exocrine pancreatic secretion by means of volume, digestive enzymes, and antibacterial activity. | exocrine pancreatic secretion contributes to limit pathogenic bacteria-associated diarrhea. bovine colostrum, used in the treatment of diarrhea, reduces symptoms originating from gut pathogenic bacteria overgrowth. we hypothesized that bovine colostrum may stimulate the exocrine pancreatic secretion. | 2009 | 19136910 |
| associations between antimicrobial resistance phenotypes, antimicrobial resistance genes, and virulence genes of fecal escherichia coli isolates from healthy grow-finish pigs. | escherichia coli often carries linked antimicrobial resistance genes on transmissible genetic elements. through coselection, antimicrobial use may select for unrelated but linked resistance or virulence genes. this study used unconditional statistical associations to investigate the relationships between antimicrobial resistance phenotypes and antimicrobial resistance genes in 151 e. coli isolates from healthy pigs. phenotypic resistance to each drug was significantly associated with phenotypic ... | 2009 | 19139228 |
| chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (igy): in vivo evaluation in a pig model of enteric colibacillosis. | in our previous study, the applicability of chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (igy) was established in a simulated gastrointestinal tract environment. the objective of the present study was to evaluate the protective efficacy of microencapsulated igy against k88+ etec (enterotoxigenic escherichia coli)-induced diarrhea in 40-day-old pigs. groups of pigs orally challenged with 10(11) cfu/ml of k88+ etec were fed with non-encapsulated igy, microencapsulat ... | 2009 | 19150135 |
| genetic loci involved in rubrifacine production in the walnut pathogen brenneria rubrifaciens. | brenneria rubrifaciens produces a unique red pigment known as rubrifacine that has been hypothesized to play a role in pathogenesis on walnut. analysis of dna flanking the tn5 insertion site in 20 rubrifacine minus (pig(-)) mutants identified three regions required for rubrifacine production. the first region was homologous to nonribosomal peptide synthetases (nrps), the second was homologous to autoinducer synthase genes (expi homologs), and the third region was homologous to the slya gene of c ... | 2009 | 19159306 |
| transmission of incn plasmids carrying blactx-m-1 between commensal escherichia coli in pigs and farm workers. | ctx-m-1-producing escherichia coli were isolated from 56 pigs, three farm personnel, two manure samples, and two air samples from two danish pig farms where an association between prophylactic ceftiofur use and the occurrence of cephalosporin resistance was previously demonstrated. human, animal, and environmental strains displayed high genetic diversity but harbored indistinguishable or closely related incn plasmids carrying bla(ctx-m-1), indicating that incn plasmids mediating cephalosporin re ... | 2009 | 19188380 |
| evaluation of baits for oral vaccination of european wild boar piglets. | the objective of this study was to develop and evaluate new baits for the oral delivery of vaccine preparations to 2-4 month-old wild boar piglets. baits were prepared using a matrix composed of wild boar feed, wheat flour, paraffin, sacarose and cinnamon-truffle powder attractant with polyethylene capsules dipped into the matrix to introduce vaccine formulation. physical stability studies demonstrated that baits were stable for at least three days at temperatures as high as 42 degrees c. recomb ... | 2009 | 18950820 |
| transgenic lettuce producing a candidate protein for vaccine against edema disease. | pig edema disease is a bacterial disease caused by shiga toxin 2e-producing escherichia coli belonging mainly to serotypes o138, o139, and o141. the b subunit of shiga toxin 2e (stx2eb) is a candidate protein for use in a vaccine against edema disease. we produced this protein in transgenic lettuce (lactuca sativa), an edible plant that can be cultivated in a factory setting. in a transient expression system, we found that ntadh 5'-untranslated region (5'-utr) functions as a translational enhanc ... | 2009 | 19584542 |
| evidence of class 1 integron transfer between escherichia coli and salmonella spp. on livestock farms. | a study was conducted to determine if homologous integrons occurred in escherichia coli and salmonella spp. within livestock production sites in the united states and thailand suggesting transfer of genetic resistance elements between those organisms. fecal samples were collected via rectal swabs from live swine in the united states and thailand, and cloacal swabs from live chickens in thailand, and killed chickens at a u.s. abattoir. isolates were derived only from farms harboring both salmonel ... | 2009 | 19630513 |
| distribution of sulfonamide resistance genes in escherichia coli and salmonella isolates from swine and chickens at abattoirs in ontario and québec, canada. | sulfonamide-resistant escherichia coli and salmonella isolates from pigs and chickens in ontario and québec were screened for sul1, sul2, and sul3 by pcr. each sul gene was distributed differently across populations, with a significant difference between distribution in commensal e. coli and salmonella isolates and sul3 restricted mainly to porcine e. coli isolates. | 2009 | 19633109 |
| contributions of o island 48 to adherence of enterohemorrhagic escherichia coli o157:h7 to epithelial cells in vitro and in ligated pig ileal loops. | o island 48 (oi-48) of escherichia coli consists of three functional gene clusters that encode urease, tellurite resistance (te(r)), and putative adhesins iha and aida-1. the functions of these clusters in enterohemorrhagic e. coli (ehec) o157:h7 infection are unknown. deletion mutants for these three regions were constructed and evaluated for their ability to adhere to epithelial cells in vitro and in ligated pig ileal loops. deletion of the te(r) gene cluster reduced the ability of the organis ... | 2009 | 19633120 |
| [the apoptosis of vero cells induced by shiga toxin 2e]. | shiga-like toxin-producing escherichia coli (stec) causes edema disease in piglets and hemolytic uremic syndrome in human. shiga-like toxins (stxs) produced by stec induce mammalian cells death via either necrosis or apoptosis. however, the ability of stx2e, separated from edema disease (stx2e), to trigger apoptosis and the sequence of intracellular signaling events have not yet been completely defined. in this study we investigated the apoptotic effects of stx2e on vero cells. | 2009 | 19637576 |
| effect of dietary level of protein and fiber on the productive performance and health status of piglets. | to study the interaction between the levels of protein and fiber on the productive performance and health status of piglets, ninety-six 35-d-old piglets (9.11 +/- 0.60 kg of bw) were placed in 32 pens of 3 animals each and allotted to 4 dietary treatments for 21 d. the 4 diets were based on rice, dairy products, and soybean meal in a 2 x 2 factorial arrangement of treatments, with 2 levels of cp (15.4 vs. 19.4%, as-fed basis) and 2 levels of dietary fiber [df; low fiber (lf) 5.3% ndf and high fi ... | 2009 | 19648494 |
| effect of immunostimulation by detoxified e. coli lipopolysaccharide combined with inactivated propionibacterium granulosum cells on porcine immunity. | the aim of this experiment was to evaluate the immunomodulating activities of inactivated propionibacterium granulosum cell walls and e. coli lipopolysaccharide (pg/lps) on porcine immunity. piglets were intramuscularly administered pg/lps (1 ml/10 kg body weight) once or twice. the function of natural killer cells, lymphocytes and neutrophils and the adjuvant effect on antibody induction by attenuated classical swine fever virus (csfv) and inactivated mycoplasma hyopneumoniae vaccination were e ... | 2009 | 19652476 |
| phenotypic and genotypic traits of shiga toxin-negative e. coli o157:h7/h(-) bovine and porcine strains. | enterohemorrhagic e. coli (ehec) o157:h7/h(-) (nonmotile) exist as shiga toxin gene (stx)-positive and stx-negative variants in patients and the environment. we analyzed molecular characteristics, phenotypes, and the phylogenetic background of three stx-negative e. coli o157:h7/h(-) strains isolated from cattle and a pig and compared them with those of human ehec and stx-negative e. coli o157:h7/h(-). all three animal strains contained flich7 and two contained eae. one eae-positive strain (o157: ... | 2009 | 19245340 |
| characterization of sulphonamide-resistant escherichia coli using comparison of sul2 gene sequences and multilocus sequence typing. | the sul2 gene encodes sulphonamide resistance (sul(r)) and is commonly found in escherichia coli from different hosts. we typed e. coli isolates by multilocus sequence typing (mlst) and compared the results to sequence variation of sul2, in order to investigate the relation to host origin of pathogenic and commensal e. coli strains and to investigate whether transfer of sul2 into different genomic lineages has happened multiple times. sixty-eight e. coli isolated in denmark and norway from diffe ... | 2009 | 19246754 |
| selection of non-aggregating vh binders from synthetic vh phage-display libraries. | the particular interest in vh antibody fragments stems from the fact that they can rival their "naturally occurring" single-domain antibody (sdab) counterparts (camelid vhhs and shark vnars) with regard to such desirable characteristics as stability, solubility, expression, and ability to penetrate cryptic epitopes and outperform them in terms of less immunogenicity, a much valued property in human immunotherapy applications. however, human vhs are typically prone to aggregation. various approac ... | 2009 | 19252860 |
| antimicrobial resistance in escherichia coli strains isolated from swiss weaned pigs and sows. | based on directive (ec) no 99/2003, monitoring programs on the development of antimicrobial resistance in bacteria from livestock are implemented in many european countries. the aim of the present study was (i) to establish comprehensive baseline data on the antimicrobial resistance situation in escherichia coli isolates obtained from healthy pigs (pooled fecal samples) originating from 60 swiss pig-breeding farms, and (ii) to analyze differences in the resistance frequency between escherichia c ... | 2009 | 19263381 |
| relatedness of escherichia coli strains with different susceptibility phenotypes isolated from swine feces during ampicillin treatment. | the aim of this study was to examine the dynamics of the development of resistance in fecal escherichia coli populations during treatment with ampicillin for 7 days in pigs. before treatment, only 6% of the isolates were ampicillin resistant, whereas more than 90% of the isolates were resistant after days 4 and 7 of treatment. ampicillin-resistant e. coli isolates were mainly multiresistant, and 53% of the isolates from the treated pigs had one phenotype that included resistance to six antibioti ... | 2009 | 19270132 |
| addition of an alginate to a modified zeolite improves hemostatic performance in a swine model of lethal groin injury. | quikclot is a zeolite-based hemostatic agent that can control severe hemorrhage through adsorption of water in an exothermic reaction. ion exchanging the calcium ions in zeolite type 5a with cations of a reduced hydration enthalpy can reduce heat generation, but its effect on the hemostatic efficacy is not clear. we developed a new compound zeolite hemostat and tested it against controls in a modified swine model of battlefield injury. | 2009 | 19276728 |
| a novel endo-glucanase from the thermophilic bacterium geobacillus sp. 70pc53 with high activity and stability over a broad range of temperatures. | a thermophilic geobacillus bacterium secreting high activity of endo-glucanase (ec 3.2.1.4) was isolated from rice straw compost supplemented with pig manure. a full-length gene of 1,104 bp, cela, encoding this glycosyl hydrolase family 5 endo-glucanase of 368 amino acids was isolated. no related gene from geobacillus has been reported previously. the recombinant cela expressed in escherichia coli had an optimal activity at 65 degrees c and ph 5.0, and it exhibited tenfold greater specific activ ... | 2009 | 19296197 |
| prevalence of enterotoxigenic escherichia coli virulence genes from scouring piglets in zimbabwe. | world-wide, enterotoxigenic escherichia coli (etec) and verotoxigenic e. coli (vtec)-induced diarrhea are economically important for porcine producers. our aim was to investigate the prevalence of toxin and fimbrial genes among e. coli isolated from diarrheic piglets from randomly selected piggeries in zimbabwe. we used multiplex pcr for screening sta, stb, lt, and stx-2e toxins. subsequently f4, f5, f6, f18 and f41 fimbriae genes were screened in toxin positive isolates. toxin positive strains ... | 2009 | 19347597 |
| influence of lactobacillus fermentum i5007 on the intestinal and systemic immune responses of healthy and e. coli challenged piglets. | the effect of feeding lactobacillus fermentum i5007 on the immune system of weaned pigs with or without e. coli challenge was determined. twenty-four weaned barrows (6.07 +/- 0.63 kg bw) were randomly assigned to one of four treatments (n = 6) in a factorial design experiment. the first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with l. fermentum i5007 (10(8) cfu/ml) at a daily dose of 20 ml. pigs in the s ... | 2009 | 19347600 |
| lactobacillus isolates from weaned piglets' mucosa with inhibitory activity against common porcine pathogens. | twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (s. aureus, b. cereus, e. coli, c. perfringens). two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. isolates 4/26 and 2/25 (identified as l. crispatus or l. amylovorus) were inhibitory against most strains of s. aureus, b. cereu ... | 2009 | 19381487 |
| determination of phosphorylation sites for nadp-specific isocitrate dehydrogenase from mycobacterium tuberculosis. | isocitrate dehydrogenase (icd) catalyzes the oxidative decarboxylation reaction of 2r,3s-isocitrate to yield 2-oxoglutarate in the tricarboxylic acid (tca) cycle. two isoforms of nadp-specific icds with the e.c number 1.1.1.42 have been annotated in the organism mycobacterium tuberculosis, monomeric icd2 and dimeric icd1. blast search against the protein data bank (pdb) database shows a marked similarity between dimeric mycobacterium tuberculosis icd1 sequence and that of sus scrofa, a cytosolic ... | 2009 | 19385702 |
| the food contaminant fumonisin b(1) reduces the maturation of porcine cd11r1(+) intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal etec infection. | consumption of food or feed contaminated with fumonisin b(1) (fb(1)), a mycotoxin produced by fusarium verticillioides, can lead to disease in humans and animals. the present study was conducted to examine the effect of fb1 intake on the intestinal immune system. piglets were used as a target and as a model species for humans since their gastro-intestinal tract is very similar. the animals were orally exposed to a low dose of fb(1) (1 mg/kg body weight fb(1)) for 10 days which did not result in ... | 2009 | 19389343 |
| refined localization of the escherichia coli f4ab/f4ac receptor locus on pig chromosome 13. | diarrhoea in newborn and weaned pigs caused by enterotoxigenic escherichia coli (etec) expressing f4 fimbriae leads to considerable losses in pig production. in this study, we refined the mapping of the receptor locus for etec f4ab/f4ac adhesion (f4bcr) by joint analysis of nordic and swiss data. a total of 236 pigs from a nordic experimental herd, 331 pigs from a swiss experimental herd and 143 pigs from the swiss performing station were used for linkage analysis. genotyping data of six known m ... | 2009 | 19392815 |
| quantification of heparan sulfate disaccharides using ion-pairing reversed-phase microflow high-performance liquid chromatography with electrospray ionization trap mass spectrometry. | the glycosaminoglycan (gag) family of biomacromolecules is composed acidic and linear chains of repeating disaccharide units. quantitative disaccharide composition analysis is essential for the study and characterization of gags. heparan sulfate and heparin consist of multiple disaccharide units and can be well-separated by ion-pairing reversed-phase microflow high-performance liquid chromatography (iprp-mf-hplc). each disaccharide can be detected and its mass confirmed by electrospray ionizatio ... | 2009 | 19402671 |
| comparison of genotypes of escherichia coli strains carrying f18ab and f18ac fimbriae from pigs. | escherichia coli carrying the f18 fimbriae colonize the small intestine of pigs and cause postweaning diarrhea and edema disease. there are 2 closely related antigenic variants of f18, f18ab, and f18ac. while f18ab-positive strains are known to be associated with edema disease, e. coli-carrying f18ac are known to cause diarrhea. one hundred ninety-eight e. coli isolates obtained from cases of diarrhea and edema disease in pigs isolated from feces or intestine were screened for the presence of th ... | 2009 | 19407090 |
| escherichia coli expressing single-chain fv on the cell surface as a potential prophylactic of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) is a causative agent of severe diarrhea which leads to death in piglets. because of the high mortality which is up to 100% in suckling piglets, ped is an important porcine disease in korea. in this study, we developed a prophylactic candidate using single-chain fvs to prevent the pedv infection. scfvs of mouse monoclonal antibody which was verified to neutralize pedv was expressed in escherichia coli expression system. after the confirmation of pedv neutral ... | 2009 | 19428826 |
| escherichia coli nissle 1917 for probiotic use in piglets: evidence for intestinal colonization. | this study was prompted to investigate the intestinal localization and colonization of orally administered escherichia coli nissle 1917 (ecn) in piglets. | 2009 | 19457029 |
| incidence of lameness and abrasions in piglets in identical farrowing pens with four different types of floor. | lameness in piglets is a major animal welfare issue. floor abrasiveness is a common cause of superficial injury in piglets in farrowing pens. the abrasion achieved may act as a gate for infections, which in turn may induce development of infectious arthritis. in this study, the influence of improvements of the floor quality and of increased ratios of straw in identical farrowing pens was measured. | 2009 | 19463187 |
| african swine fever virus ap endonuclease is a redox-sensitive enzyme that repairs alkylating and oxidative damage to dna. | african swine fever virus (asfv) encodes an ap endonuclease (pe296r) which is essential for virus growth in swine macrophages. we show here that the dna repair functions of pe296r (ap endonucleolytic, 3'-->5' exonuclease, 3'-diesterase and nucleotide incision repair (nir) activities) and dna binding are inhibited by reducing agents. protein pe296r contains one intramolecular disulfide bond, whose disruption by reducing agents might perturb the interaction of the viral ap endonuclease with the dn ... | 2009 | 19464038 |
| activation of peroxisome proliferator-activated receptor-gamma potentiates pro-inflammatory cytokine production, and adrenal and somatotropic changes of weaned pigs after escherichia coli lipopolysaccharide challenge. | our previous study demonstrated mrna and protein expression of peroxisome proliferator-activated receptor-g (ppar-g) in the immune system of weaned pigs. in this report, to test the hypothesis that activation of ppar-g in immune system modulates inflammatory response, and adrenal and somatotropic responses associated with immune challenge, we administered intraperitoneally ppar-g agonist and/or antagonist in weaned pigs subjected to escherichia coli lipopolysaccharide (lps) challenge. unexpected ... | 2009 | 19474210 |
| antimicrobial use in the australian pig industry: results of a national survey. | to describe how various antimicrobials are used in commercial pig herds in australia and for what disease conditions. | 2009 | 19489779 |
| ctx-m-type beta-lactamase in escherichia coli isolated from sick animals in korea. | about 408 escherichia coli were isolated from sick farm animals and pets during 2003-2006. of these isolates, four strains showed resistance to third-generation cephalosporins. the bla(ctx-m-14) gene was encountered in three e. coli strains, each of which were isolated from two cows and a dog, respectively, and bla(ctx-m-15) was identified in an e. coli isolated from a pig. all bla(ctx-m) genes were found to be transferred. the pulsed-field gel electrophoresis pattern indicated that the ctx-m-ty ... | 2009 | 19496675 |
| resistance patterns and detection of aac(3)-iv gene in apramycin-resistant escherichia coli isolated from farm animals and farm workers in northeastern of china. | the aminoglycoside apramycin has been used widely in animal production in china since 1999. this study was aimed to investigate the resistance pattern of apramycin-resistant escherichia coli isolated from farm animals and farm workers in northeastern of china during 2004-2007 and to determine whether resistance to apramycin was mediated by plasmid containing the aac(3)-iv gene and the mode for the transfer of genetic information between bacteria of farm animals and farm workers. thirty six e. co ... | 2009 | 19501867 |
| molecular analysis of escherichia coli from retail meats (2002-2004) from the united states national antimicrobial resistance monitoring system. | the origins and virulence potential of meat product-associated escherichia coli are undefined. | 2009 | 19508167 |
| global spread of mobile antimicrobial drug resistance determinants in human and animal escherichia coli and salmonella strains causing community-acquired infections. | antimicrobial drugs used in human infection treatment and animal husbandry may select for drug-resistant bacterial pathogens, which are increasingly observed worldwide. we sought to examine the extent to which identical mobile drug resistance elements are shared across common pathogens isolated from human and animal sources. | 2009 | 19538087 |
| effects of phenyllactic acid on growth performance, nutrient digestibility, microbial shedding, and blood profile in pigs. | ninety weanling pigs in exp. 1 (6.27 +/- 0.73 kg; 21 d of age) and 96 growing pigs in exp. 2 (21.73 kg +/- 1.29 kg; 56 d of age) were used in two 42-d experiments to evaluate the effect of phenyllactic acid (pla) on growth performance, apparent total tract digestibility (attd) of dm and n, fecal ph value, microbial shedding, and blood profiles. in exp. 1, the 3 dietary treatments were 1) negative control (nc), 2) positive control (pc), nc + antibiotics, and 3) pla, nc + 0.5% pla. in exp. 2, diet ... | 2009 | 19542505 |
| expression of porcine parvovirus vp2 gene requires codon optimized e. coli cells. | porcine parvovirus (ppv) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. the gene coding for the capsid protein vp2 of ppv was amplified and inserted into the plasmid pet-32a (+), which was then used to transform escherichia coli rosetta, the capsid protein of ppv was fused to a polyhistidine tag, and the position of the affinity tag is in n-terminus. vp2 was expressed using different expression host bacteria, including e. coli bl21, and ... | 2009 | 19543964 |
| factors related to the carriage of verocytotoxigenic e. coli, salmonella, thermophilic campylobacter and yersinia enterocolitica in cattle, sheep and pigs at slaughter. | a 12-month abattoir study was undertaken from january 2003. we collected 7492 intestinal samples from cattle, sheep and pigs at slaughter. rectal samples were taken from cattle and sheep and caecal samples from pigs. they were examined for verocytotoxigenic e. coli (vtec) o157, salmonella, thermophilic campylobacter and yersinia enterocolitica. data were collected on the animal from which the sample came and this information was analysed to look at potential risk factors for carriage of these or ... | 2009 | 19203406 |
| a pig-human comparative rh map comprising 20 genes on pig chromosome 13q41 that harbours the etec f4ac receptor locus. | the enterotoxigenic escherichia coli (etec) f4ac is a major cause of diarrhoea in newborn and young pigs. the locus for the intestinal etec f4ac receptor (f4acr) has been mapped to pig chromosome (ssc) 13q41 with known homology to human chromosome (hsa) 3q21 and q29. however, the causative gene and mutation(s) remain unknown. the aim of this study was to characterize gene-derived markers on ssc13q41 for fine mapping of the f4acr locus, and construct a high-resolution pig-human comparative map to ... | 2009 | 19207927 |
| concentration and bioavailability of ciprofloxacin and teicoplanin in the cornea. | to investigate the concentration and bioavailability of ciprofloxacin and teicoplanin in the cornea. | 2009 | 19218614 |
| epr spectroscopy and electrospray ionization mass spectrometry reveal distinctive features of the iron site in leukocyte 12-lipoxygenase. | the procedure for the expression and purification of recombinant porcine leukocyte 12-lipoxygenase using escherichia coli [k.m. richards, l.j. marnett, biochemistry 36 (1997) 6692-6699] was updated to make it possible to produce enough protein for physical measurements. electrospray ionization tandem mass spectrometry confirmed the amino acid sequence. the redox properties of the cofactor iron site were examined by epr spectroscopy at 25k following treatment with a variety of fatty acid hydroper ... | 2009 | 19683507 |
| assessment of shiga toxin-producing escherichia coli isolates from wildlife meat as potential pathogens for humans. | a total of 140 shiga toxin-producing escherichia coli (stec) strains from wildlife meat (deer, wild boar, and hare) isolated in germany between 1998 and 2006 were characterized with respect to their serotypes and virulence markers associated with human pathogenicity. the strains grouped into 38 serotypes, but eight o groups (21, 146, 128, 113, 22, 88, 6, and 91) and four h types (21, 28, 2, and 8) accounted for 71.4% and 75.7% of all stec strains from game, respectively. eighteen of the serotype ... | 2009 | 19700552 |
| cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in henan. | to evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ... | 2009 | 19732142 |
| arginine-vasopressin attenuates beneficial norepinephrine effect on jejunal mucosal tissue oxygenation during endotoxinaemia. | the objective of the present study was to investigate the effects of increasing doses of norepinephrine (ne) with or without arginine-vasopressin (avp) on intestinal oxygen supply and jejunal mucosal tissue oxygen tension in an acute endotoxic pig model. | 2009 | 19720610 |
| occurrence and characteristics of class 1 and class 2 integrons in resistant escherichia coli isolates from animals and farm workers in northeastern china. | the purpose of this study was to investigate the incidence of class 1 and class 2 integrons and the presence of gene cassettes in resistant escherichia coli isolates from farm animals and farm workers in northeastern china during 2004-2007. most of the e. coli isolates from the farm workers and animals were mutiresistant and showed high resistance to aminoglycosides, chloramphenicol, amoxicillin, sulfanilamide, and quinolones. integrons were found in 56.8% of the resistant e. coli isolates from ... | 2009 | 19728782 |
| escherichia coli transport from surface-applied manure to subsurface drains through artificial biopores. | bacteria transport in soils primarily occurs through soil mesopores and macropores (e.g., biopores and cracks). field research has demonstrated that biopores and subsurface drains can be hydraulically connected. this research was conducted to investigate the importance of surface connected and disconnected (buried) biopores on escherichia coli (e. coli) transport when biopores are located near subsurface drains. a soil column (28 by 50 by 95 cm) was packed with loamy sand and sandy loam soils to ... | 2009 | 19875797 |
| characterization of selected nutrients and bacteria from anaerobic swine manure lagoons on sow, nursery, and finisher farms in the mid-south usa. | swine (sus scrofa domestica) production in the mid-south usa comprises sow, nursery, and finisher farms. a 2007 packing plant closure started a regional shift from finisher to sow and nursery farms. changes in manure stored in lagoons and land-applied as fertilizer were expected but were unknown because nutrient and bacterial levels had not been characterized by farm type. the objectives of this study were to quantify selected nutrients and bacteria, compare levels by farm types, and project imp ... | 2009 | 19875798 |
| antibiotic resistant bacterial profiles of anaerobic swine lagoon effluent. | although land application of swine (sus scrofa) manure lagoon effluent is a common and effective method of disposal, the presence of antibiotic-resistant bacteria, both pathogenic and commensal can complicate already understood issues associated with its safe disposal. the aim of this study was to assess antibiotic resistance in swine lagoon bacteria from sow, nursery, and finisher farms in the southeastern united states. effluents from 37 lagoons were assayed for the presence of escherichia col ... | 2009 | 19875799 |
| wild boars as reservoirs of extended-spectrum beta-lactamase (esbl) producing escherichia coli of different phylogenetic groups. | esbl-producing e. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in portugal. the esbl types identified by pcr and sequencing were bla(ctx-m-1) (6 isolates) and bla(ctx-m-1) + bla(tem1-b) (2 isolates). further resistance genes detected included tet (a) or tet (b) (in three tetracycline-resistant isolates), aad a (in three streptomycin-resistant isolates), cml a (in one chloramphenicol-resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all s ... | 2009 | 19810044 |
| analysis of the aida-i gene sequence and prevalence in escherichia coli isolates from pigs with post-weaning diarrhoea and oedema disease. | three hundred and twenty-four strains of escherichia coli isolated from weaned pigs with diarrhoea or oedema disease in eastern china were screened by multiplex pcr for the presence of the gene encoding adhesin involved in diffuse adhesion i (aida-i). two aida-i positive strains were subjected to analysis of the nucleotide sequence of the complete orfa and orfb of the aida gene. the aida-i positive e. coli isolates were also assessed for five fimbriae (f4, f5, f6, f18 and f41) by monoclonal anti ... | 2009 | 18077196 |
| epoprostenol improves mucosal tissue oxygen tension in an acute endotoxemic pig model. | the objective of the present study was to determine the effects of increasing dosages of continuously infused epoprostenol (pgi), a prostacyclin analog, on intestinal oxygen supply and jejunal mucosal tissue oxygen tension in an acute endotoxic pig model. jejunal mucosal tissue po2, oxygen saturation of jejunal microvascular hemoglobin, and gut microvascular blood flow were investigated. systemic hemodynamic variables, mesenteric-venous and systemic acid base and blood gas variables, and lactate ... | 2009 | 18520707 |
| a tryptophan-enriched diet improves feed intake and growth performance of susceptible weanling pigs orally challenged with escherichia coli k88. | we tested the effect of trp addition to a standard weaning diet and oral challenge with enterotoxigenic escherichia coli k88 (etec) on growth and health of piglets susceptible or nonsusceptible to the intestinal adhesion of etec. sixty-four pigs weaned at 21 d of age were divided into 3 groups based on their ancestry and bw: a control group of 8 pigs fed a basal diet (b), the first challenged group of 28 pigs fed b diet (bch), and the second challenged group of 28 pigs fed a diet with trp (trpch ... | 2009 | 18791156 |
| associations between reported on-farm antimicrobial use practices and observed antimicrobial resistance in generic fecal escherichia coli isolated from alberta finishing swine farms. | multilevel random intercept logistic and poisson regression were used to model associations between various antimicrobial use practices and resistance to individual and multiple antimicrobials among generic fecal escherichia coli isolated from alberta finishing swine. in-feed antimicrobial use in finishers was significantly associated with increased risk of resistance to ampicillin, chloramphenicol, streptomycin, and sulfisoxazole in generic e. coli isolates. chlortetracycline use in grower rati ... | 2009 | 19041147 |
| inhibition of complement and cd14 attenuates the escherichia coli-induced inflammatory response in porcine whole blood. | the innate immune response is a double-edged sword in systemic inflammation and sepsis. uncontrolled or inappropriate activation can damage and be lethal to the host. several studies have investigated inhibition of downstream mediators, including tumor necrosis factor alpha (tnf-alpha) and interleukin-1beta (il-1beta). emerging evidence indicates that upstream inhibition is a better therapeutic approach for attenuating damaging immune activation. therefore, we investigated inhibition of two cent ... | 2009 | 19047409 |
| isolation and characterization of intestinal escherichia coli clones from wild boars in germany. | our understanding of the composition of escherichia coli populations in wild boars is very limited. in order to obtain insight into the e. coli microflora of wild boars, we studied e. coli isolates from the jejunums, ileums, and colons of 21 wild boars hunted in five geographic locations in germany. ten isolates per section were subjected to clonal determination using pulsed-field gel electrophoresis. one representative isolate per clone was further investigated for virulence traits, phylogeneti ... | 2009 | 19060173 |
| supplementation of diets for gestating sows with zinc amino acid complex and gastric intubation of suckling pigs with zinc-methionine on mineral status, intestinal morphology and bacterial translocation in lipopolysaccharide-challenged early-weaned pigs. | eighty male pigs from 20 litters were used to evaluate dietary addition of 250mg/kg of zn from zinc amino acid complex (znaa) to sows during the last trimester of gestation and gastric intubation of 40mg zn from soluble zinc methionine (znmet) to suckling pigs at birth and on day 7 and 14 (weaning) on small intestinal morphology, zn status and bacterial translocation in early-weaned pigs. at weaning, pigs were challenged with an intramuscular injection of saline without or with 120microg/kg bw o ... | 2009 | 19062056 |
| characterization of monoclonal antibody against replication-associated protein of porcine circovirus. | the replication-associated (rep) protein of porcine circovirus (pcv) was suggested to play an essential role in the replication and translation of viral dna. in this study, one monoclonal antibody (mab) specific for rep protein of porcine circovirus type 1 (pcv1), two mabs against rep protein of porcine circovirus type 2 (pcv2), and five mabs to both rep protein of pcv1 and pcv2 were generated using, respectively, rep protein of pcv1 and pcv2 expressed in escherichia coli as an immunogen. wester ... | 2009 | 19072659 |
| biorecognition of escherichia coli k88 adhesin for glycated porcine albumin. | escherichia coli (e. coli) that expresses galactose-reactive lectins, like k88 adhesin, causes high mortality among piglets. carbohydrates that compete for adhesion could serve as an alternative for disease prevention. porcine serum albumin (psa) was modified by non-enzymatic glycation with lactose to produce psa-lac or psa-glc beta (1-4) gal, as confirmed by reduction of available free amino groups, increased molecular mass and by ricinus communis lectin recognition. e. coli k88 binds to psa-la ... | 2009 | 19101589 |
| expression, purification, and characterization of recombinant ns-1, the porcine parvovirus non-structural protein. | the non-structural protein ns-1 of porcine parvovirus (ppv) could be a useful antigen for differentiating pigs infected with ppv from those vaccinated with inactivated whole virus. ns-1 was expressed in escherichia coli using the pet-32a (+) vector. the fusion protein, which was expressed at a high level, was similar antigenically to the native ns-1 protein as determined by western blot assay using polyclonal antibodies from pigs vaccinated with alive ppv vaccine. a simple procedure was used to ... | 2009 | 19101593 |
| characterization of a novel macrolide efflux gene, mef(b), found linked to sul3 in porcine escherichia coli. | the aim of this study was to characterize a putative novel macrolide efflux gene located in the vicinity of sul3 in porcine escherichia coli. | 2009 | 19131424 |
| genotypic and phenotypic characterisation of escherichia coli strains associated with porcine pyelonephritis. | urinary tract infection (uti) is a severe problem in humans as well as in many domestic animals like pigs. the most frequent infectious agent in uti is uropathogenic escherichia coli. such strains have been extensively characterised with respect to virulence and fitness factors as well as clonal type when it comes to human isolates. however, relatively little has been done to characterise the corresponding porcine strains. on this background we have analysed 20 porcine pyelonephritis e. coli str ... | 2009 | 18835113 |
| parallel isotope-based quantification of modified trna nucleosides. | 2009 | 19746495 | |
| bacterial effector binding to ribosomal protein s3 subverts nf-kappab function. | enteric bacterial pathogens cause food borne disease, which constitutes an enormous economic and health burden. enterohemorrhagic escherichia coli (ehec) causes a severe bloody diarrhea following transmission to humans through various means, including contaminated beef and vegetable products, water, or through contact with animals. ehec also causes a potentially fatal kidney disease (hemolytic uremic syndrome) for which there is no effective treatment or prophylaxis. ehec and other enteric patho ... | 2009 | 20041225 |
| thermodynamic analysis of the structure-function relationship in the total dna-binding site of enzyme-dna complexes. | both helicases and polymerases perform their activities when bound to the nucleic acids, that is, the enzymes possess a nucleic acid-binding site. functional complexity of the helicase or the polymerase action is reflected in the intricate structure of the total nucleic acid-binding site, which allows the enzymes to control and change their nucleic acid affinities during the catalysis. understanding the fundamental aspects of the functional heterogeneity of the total nucleic acid-binding site of ... | 2009 | 21603116 |