Publications

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prognostic value of mucin 4 expression in colorectal adenocarcinomas.mucin 4 (muc4) is aberrantly expressed in colorectal adenocarcinomas (crcs) but its prognostic value is unknown.201020564074
radio frequency radiation causes no nonthermal damage in enzymes and living cells.the ability of radio frequency radiation (rfr) to exert irreversible nonthermal (i.e., not caused by accompanying heat) effects on biologics has been widely debated due to a relative paucity of comprehensive critical details in published reports dealing with this issue. in this study, we used rigorous control over experimental conditions to determine whether continuous rfr nonthermally affects commercially important enzymes and live bacterial and human cells using three most commonly used freque ...201020572294
[the striopallidal projections of the zona incerta in the dog diencephalon].the projections of individual sectors of the zona incerta (zi) in dog diencephalon to the functionally different striatal segments were studied by the method of the antero- and retrograde axonal transport of horseradish peroxidase. the data on the interconnections of zi with all striopallidal structures, obtained by the author before and in the present work were summarized. among all the striatal nuclei, the the rostral, ventral and caudal zi sectors contained the neurons that projected their fi ...201020572386
cholecystokinin/cholecystokinin-1 receptor-mediated peripheral activation of the afferent vagus by enteral nutrients attenuates inflammation in rats.the current study investigates activation of the nutritional anti-inflammatory pathway by lipid-rich nutrition.201020585240
determination of plasma and serum l-lysine using l-lysine epsilon-oxidase from marinomonas mediterranea nbrc 103028(t).this article describes a successful application of l-lysine epsilon-oxidase (ec 1.4.3.20) for l-lysine determination. l-lysine epsilon-oxidase was isolated from culture supernatant of marinomonas mediterranea nbrc 103028(t) and was used for l-lysine determination. comparison of the characteristics of l-lysine epsilon-oxidase with l-lysine alpha-oxidase, a commercial enzyme used for l-lysine determination, suggests that the use of l-lysine epsilon-oxidase would be more valuable for the determinat ...201020599635
a direct competitive enzyme-linked immunosorbent assay by antibody coated for diethyl phthalate analysis.a direct competitive enzyme-linked immunosorbent assay (elisa) has been developed for detection of diethyl phthalate (dep). protein-hapten conjugate was synthesized to produce polyclonal antibodies against dep. experimental parameters were optimized, including immunoreaction conditions, the dilution ratio of horseradish peroxidase (hrp)-antigen conjugate, time of the antibody coated, effect of ph, and ionic strength. the limit of detection was 0.096ng/ml, and the linear range was 0.1-3500ng/ml w ...201020599639
igg2 dominancy and carbohydrate recognition specificity of c3h/he mouse antibodies directed to cross-reactive carbohydrate determinants (ccds) bearing beta-(1,2)-xylose and alpha-(1,3)-fucose.few common carbohydrate epitopes consisting of terminal beta-(1,2)-xylose and/or alpha-(1,3)-fucose residues are shared by a variety of glycoproteins from plants, insects and parasitic worms, termed cross-reactive carbohydrate determinant (ccd), and frequently recognized by ige antibodies of patients with food and/or respiratory allergy, though clinical relevancy of such ccd-specific ige is still controversial. attention has also been focused on ccds from the undesired post-translational modific ...201020600324
antioxidant and pro-oxidant properties of acylated pelargonidin derivatives extracted from red radish (raphanus sativus var. niger, brassicaceae).the antioxidant and pro-oxidant potential of an extract from red radish, in which the major compounds were acylated pelargonidin derivatives, were assessed with a variety of assays in vitro. the extract appeared to form a complex with fe(3+) or cu(2+). it displayed a concentration-dependant reducing power (1.16od(700 nm) at a concentration of 4mm) and scavenging effect against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals (with ic(50)=1.74 +/- 0.03 mm). it could promote the cle ...201020600520
immunoassay for quantifying squamous cell carcinoma antigen in serum.although the benefits of quantifying serum squamous cell carcinoma antigen (scca) have been reported, scca reagents were no longer available in the us by the late 1990s. because scca quantification still has demonstrated clinical utility, we developed and validated a microtiter plate-based elisa for measuring scca in serum.201020601447
pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin.in histochemistry and cytochemistry, horseradish peroxidase-labeled lectins are often used as probes for the localization of carbohydrates in cells and tissues. in transmission electron microscopy, the most commonly used procedure for detection of carbohydrates is lectin-gold labeling. horseradish peroxidase catalyzes the formation of insoluble polymerized diaminobenzidine which on exposure to osmium tetroxide forms osmium black, a compound visible in the electron microscope, making horseradish ...201020602219
real-time monitoring and kinetic parameter estimation of the affinity interaction of jartinm and rartinm with peroxidase glycoprotein by the electrogravimetric technique.artinm is a d-mannose binding lectin that has been arousing increasing interest because of its biomedical properties, especially those involving the stimulation of th1 immune response, which confers protection against intracellular pathogens. the potential pharmaceutical applications of artinm have motivated the production of its recombinant form (rartinm) so that it is important to compare the sugar-binding properties of jartinm and rartinm in order to take better advantage of the potential app ...201020605432
spinal motor neuronal degeneration after knee joint immobilization in the guinea pig.this study used a unilateral knee joint immobilization model in adult guinea pigs to test the hypothesis that retrograde degeneration of motor neurons in the spinal cord is the result of attenuation of knee joint activities.201020605551
recombinant peroxidase production in species of lepidoptera frequently found in argentina.horseradish peroxidase isozyme c (hrpc) is an important commercial biocatalyst. in this study, a screening of different lepidopteran species frequently found in argentina to produce this protein was carried out. two recombinant viruses were constructed: acmnpv hrpc polyhedrin-minus (occ-), an intrahemocelical infective virus; and acmnpv hrpc polyhedrin-plus (occ+), to achieve an oral infective baculovirus. each lepidopteran species was infected either with acmnpv hrpc occ- or acmnpv hrpc occ+ an ...201020615485
disposable amperometric magnetoimmunosensors for the specific detection of streptococcus pneumoniae.disposable amperometric magnetoimmunosensors, based on the use of functionalized magnetic beads and gold screen-printed electrodes, have been developed for the selective detection and quantification of streptococcus pneumoniae. a specific antibody prepared against a serotype 37 s. pneumoniae strain, selected by flow cytometry among seven anticapsular or antisomatic antibodies, was linked to protein a-modified magnetic beads and incubated with bacteria. the same antibody, conjugated with horserad ...201020615685
histochemical evaluation of human prostatic tissues with cratylia mollis seed lectin.lectins, proteins which selectively recognize carbohydrates, have been used in histochemistry for the evaluation of changes in glycosylation in processes of cellular differentiation and/or dedifferentiation. cratylia mollis seed lectins (cramoll 1,4 and cramoll 3), conjugated to horseradish peroxidase, were used as histochemical probes in human prostate tissues: normal (np), hyperplasia (bph), and prostate carcinoma (pca). the staining pattern of con-a and cramoll 1,4 in bph was more intense tha ...201020617135
montelukast treatment (cysteinyl leukotriene receptor antagonist) in a model of food allergy: modifications in lymphatic cell population from rectal mucosa.the aim is to determine immunopathological modifications in rectal mucosa from rabbits after local challenge in ovalbumin (ova) sensitized animals previously treated with montelukast.201020617862
noncompetitive immunoassay for follicle-stimulating hormone in human serum using capillary electrophoresis with chemiluminescence detection.a noncompetitive immunoassay based on capillary electrophoresis (ce) with chemiluminescence (cl) detection has been developed for the determination of follicle-stimulating hormone (fsh) in human serum. the work involved the development of separation and cl conditions allowing for routine analysis of serum samples. in this study, horseradish peroxidase (hrp)-labeled monoclonal anti-fsh can catalyze the luminol-hydrogen peroxide reaction. the determined fsh can react with an excessive amount of hr ...201020623406
online coupling of reverse-phase and hydrophilic interaction liquid chromatography for protein and glycoprotein characterization.we have developed a novel system for coupling reverse-phase (rp) and hydrophilic interaction liquid chromatography (hilic) online in a micro-flow scheme. in this approach, the inherent solvent incompatibility between rp and hilic is overcome through the use of constant-pressure online solvent mixing, which allows our system to perform efficient separations of both hydrophilic and hydrophobic compounds for mass spectrometry-based proteomics applications. when analyzing the tryptic digests of bovi ...201020632160
feeding on poplar leaves by caterpillars potentiates foliar peroxidase action in their guts and increases plant resistance.peroxidases (pods) are believed to act as induced and constitutive defenses in plants against leaf-feeding insects. however, little work has examined the mode of action of pods against insects. putative mechanisms include the production of potentially antinutritive and/or toxic semiquinone free radicals and quinones (from the oxidation of phenolics), as well as increased leaf toughness. in this study, transgenic hybrid poplar saplings (populus tremula × populus alba) overexpressing horseradish p ...201020680646
rapid quantification of melamine in milk using competitive 1,1'-oxalyldiimidazole chemiluminescent enzyme immunoassay.a novel competitive 1,1'-oxalyldiimidazole (odi) chemiluminescent enzyme immunoassay (cleia) was developed as a method for rapid and simple screening of melamine in milk. fat existing in milk acts as an inhibitor in the competitive binding interaction of melamine and anti-melamine in the presence of melamine-conjugated horseradish peroxidase. thus, the calibration curve and sensitivity of competitive odi cleia for the quantification of melamine in fat free milk were wider and better than those i ...201020683522
in situ electrochemical imaging of membrane glycan expression on micropatterned adherent single cells.a scanning electrochemical microscopic (secm) method for in situ imaging of four types of membrane glycan motifs on single adherent cells was proposed using bgc-823 human gastric carcinoma (bgc) cells as the model. these adherent cells were first micropatterned in the microwell of poly(dimethylsiloxane) membrane for precisely controlling the localized surface interaction, and the membrane glycans were then specifically recognized with corresponding lectins labeled with horseradish peroxidase (hr ...201020684525
a simple and rapid competitive enzyme-linked immunosorbent assay (celisa) for high-throughput measurement of secretory immunoglobulin a (siga) in saliva.a simple competitive enzyme-linked immunosorbent assay (celisa) was established for rapid measurement of secretory immunoglobulin a (siga) in saliva. the method was based on competitive reaction between the immobilized iga and free iga in the solution for the limited amount of horseradish peroxidase-conjugated rabbit anti-human iga. in comparison with the conventionally used sandwich elisa, the celisa is simpler, low-cost, and shows better reproducibility since it is not affected by the variatio ...201020685490
single-cell detection using a thin film transistor photosensor with micro-partitions.a thin film transistor (tft) photosensor was applied to single-cell detection by identifying cell surface molecules based on chemiluminescence. micro-partitions were directly fabricated on the tft photosensor surface by photolithography. the surface of each pixel was surrounded by 25 μm-height partitions, forming areas of approximately 30 μm × 30 μm for cell entrapment and photosensing. visualization of individual jm cells, stained with mouse anti-human cd8 igg1 primary antibody and horseradish ...201020694269
detection of quorum sensing molecules in burkholderia cepacia culture supernatants with enzyme-linked immunosorbent assays.the burkholderia cepacia complex (bcc) employs a quorum sensing (qs) mechanism which is a cell density-dependent bacterial communication system to regulate certain gene expressions. as with many other gram-negative bacteria, burkholderia cepacia species use (n-acyl-)homoserine lactones (ahls or hsls) as signalling molecules. because of the essential role of qs in bacterial behavior, the aim of this study was to demonstrate the applicability of our in-house-developed enzyme-linked immunosorbent a ...201020694722
[treatment of spinal cord injury by mild hypothermia combined with bone marrow mesenchymal stem cells transplantation in rats].bone marrow mesenchymal stem cells (bmscs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on bmscs transplantation, proliferation, and differentiation. to investigate the effect of bmscs transplantation and mild hypothermia on repair of rat spinal cord injury (sci).201020695375
quantum dots based electrochemiluminescent immunosensor by coupling enzymatic amplification with self-produced coreactant from oxygen reduction.a highly sensitive competitive immunosensor based on the electrochemiluminescence (ecl) of quantum dots (qds) was proposed by coupling with an enzymatic amplification. the fabrication process of the immunosensor was traced with atomic force microscopic images and electrochemical impedance spectra. the strong cathodic ecl emission of the immobilized qds could be detected at a relatively low emission potential. the reduction of dissolved oxygen during the cathodic process provided a self-produced ...201020695454
expression of the carbohydrate recognition domain of fimh and development of a competitive binding assay.uropathogenic escherichia coli (upec) is the primary cause of urinary tract infections (utis). in the first step of this infective process, the virulence factor fimh located on type 1 pili allows upec to specifically adhere to oligosaccharides, which are part of glycoproteins on the urinary bladder mucosa. this initial step prevents the clearance of e. coli from the urinary tract and enables the invasion of the host cells. because fimh antagonists can block this interaction, they exhibit a promi ...201020705050
trace detection of picloram using an electrochemical immunosensor based on three-dimensional gold nanoclusters.picloram, a herbicide widely used for broadleaf weed control, is persistent and mobile in soil and water with adverse health and environmental effects. it is important to develop a sensitive method for accurate detection of trace picloram in the environment. in this article, a type of ordered three-dimensional (3d) gold (au) nanoclusters obtained by two-step electrodeposition using the spatial obstruction/direction of the polycarbonate membrane is reported. bovine serum albumin (bsa)-picloram wa ...201020709012
the role of stiffness of gelatin-hydroxyphenylpropionic acid hydrogels formed by enzyme-mediated crosslinking on the differentiation of human mesenchymal stem cell.we report the stimulation of neurogenesis and myogenesis of human mesenchymal stem cells (hmscs) on the surfaces of biodegradable hydrogels with different stiffness. the hydrogels were composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugate were formed using the oxidative coupling of phenol moieties catalyzed by hydrogen peroxide (h(2)o(2)) and horseradish peroxidase (hrp). the storage modulus of the hydrogels was readily tuned from 600 to 12800 pa. it was found that the stiffness of ...201020709390
modification of the heme active site to increase the peroxidase activity of thermophilic cytochrome p450: a rational approach.the site specific mutants of the thermophilic p450 (p450 175a1 or cyp175a1) were designed to introduce residues that could act as acid-base catalysts near the active site to enhance the peroxidases activity. the leu80 in the distal heme pocket of cyp175a1 was located at a position almost equivalent to the glu183 that is involved in stabilization of the ferryl heme intermediate in chloroperoxidase (cpo). the leu80 residue of cyp175a1 was mutated with histidine (l80h) and glutamine (l80q) that cou ...201020709408
immobilization of horseradish peroxidase on nanoporous copper and its potential applications.nanoporous copper (npc) with a pore size of 100-200 nm was prepared by simply dealloying al(60)cu(40) alloy in a 5 wt.% hcl solution. the npc was characterized by scanning electron microscopy and nitrogen adsorption techniques. horseradish peroxidase (hrp) was immobilized on npc by adsorption. compared with free enzyme, the thermal stability of the immobilized enzyme was greatly improved due to the multiple attachments between the enzyme molecule and the npc surface. after 2h incubation at 50 de ...201020709542
oxidation reactions mediated by single-walled carbon nanotubes in aqueous solution.how single-walled carbon nanotubes (swcnt) function in redox reactions may be related to their behaviors in the induction of oxidative stress. herein, oxidation of several biologically relevant reducing agents in the presence of swcnt was studied in aqueous solutions. the selected reductants included a common indicator for intracellular reactive oxygen species (ros) (2,7-dichlorodihydrofluorescein), small antioxidants (vitamin c, trolox, and cysteine), and a high-molecular-weight ros scavenger ( ...201020715868
advent of innovative chemiluminescent enzyme immunoassay.using 1,1'-oxalyldiimidazole (odi) chemiluminescence detection, a new chemiluminescent enzyme immunoassay (cleia) was developed to quantify prostate specific antigen (psa) in human serum. the results observed in odi cleia were compared with those obtained in commercially available enzyme linked immunosorbent assay (elisa), fluorescence enzyme immunoassay (feia), and luminol cleia. psa complex-conjugated horseradish peroxidase (hrp) was formed from one-step sandwich immunoreaction of psa, psa pri ...201020739174
qsar-assisted design of an environmental catalyst for enhanced estrogen remediation.a quantitative structure-activity relationship (qsar) was used to streamline re-design of a model environmental catalyst, horseradish peroxidase (hrp), for enhanced reactivity towards a target pollutant, steroid hormone 17β-estradiol. this qsar, embodying relationship between reaction rate and intermolecular binding distance, was used in silico to screen for mutations improving enzyme reactivity. eight mutations mediating significant reductions in binding distances were expressed in saccharomyce ...201020797763
polystyrene microspheres based sandwich immunosensor using cdte nanoparticles amplification and ultrasensitive flow-injection chemiluminescence detection.in this paper we propose a specific sandwich immunoassay method for human-immunoglobulin g (higg). this immunoassay protocol takes advantage of sandwich binding of primary and secondary antibodies for increased specificity. polystyrene microspheres (ps) serve as immobilizing support, site for sandwich immunoassay and then subsequently used for chemiluminescence (cl) detections. in this sandwich immunoassay, ps microspheres were modified with the primary anti-higg (ab1) via electrostatic interact ...201020801005
a secondary antibody format chemiluminescence immunoassay for the determination of estradiol in human serum.a competitive immunoassay for estradiol (e2) based on secondary antibody format was established. the donkey anti-rabbit igg was used as the secondary antibody to coat micro-plates, and the horseradish peroxidase (hrp)-luminol-h(2)o(2) chemiluminescent system with high sensitivity was chosen as the detection system. the addition of sodium trichloroacetate (ccl(3)coona) in the enzyme buffer as a replaceable packing material can realize directly analysis of e2 in human serum without extraction, whi ...201020801358
in vitro studies of dna damage caused by tricyclic antidepressants: a role of peroxidase in the side effects of the drugs.studies show that tricyclic antidepressants prescribed for migraines, anxiety, and child enuresis have numerous adverse effects in living cells. one of the undesired outcomes observed under treatment with these drugs is dna damage. however, the mechanisms underlying damage have yet to be elucidated. we performed in vitro studies of the dna damage caused by four tricyclic antidepressants: imipramine, amitriptyline, opipramol, and protriptyline. we focused particularly on the dna damage aided by p ...201020804147
lipid peroxidation and generation of hydrogen peroxide in frozen-thawed ram semen cryopreserved in extenders with antioxidants.the objective of this study was to evaluate the effect of addition of the antioxidants trolox and catalase to a ram semen cryopreservation extender on lipid peroxidation and hydrogen peroxide generation on the extender and in the thawed semen. semen was collected from 23 santa inês rams (one ejaculate per ram) and diluted at 32°c to a concentration of 400×10⁶ cells/ml in one of the following solution: tris-egg yolk extender (control), or the same extender supplemented with either 50μm trolox/10⁸ ...201020813469
fluorometric assay of oleate-activated phospholipase d isoenzyme in membranes of rat nervous tissue and human platelets.phospholipase d plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. oleate-activated phospholipase d was the first mammalian phospholipase d isoform to be discovered but is the least known. the study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase d activity in different biological materials. the brain cortex of male wistar rats, cultured rat brain astrocytes, and human platelets were proc ...201020835407
tresyl-based conjugation of protein antigen to lipid nanoparticles increases antigen immunogenicity.the present studies were aimed at investigating the engineering of nps with protein-conjugated-surfactant at their surface. in order to increase the immunogenicity of a protein antigen, brij 78 was functionalized by tresyl chloride and then further reacted with the primary amine of the model proteins ovalbumin (ova) or horseradish peroxide (hrp). the reaction yielded brij 78-ova and brij 78-hrp conjugates which were then used directly to form np-ova or np-hrp using a one-step warm oil-in-water m ...201020837122
resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells.a large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. the cells were identified as perivascular resident macrophages (pvms), as they were positive for several macrophage surface molecules including f4/80, cd68, and cd11b. the macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor ...201020838812
development of ultra-sensitive soybean peroxidase-based cl-elisa for the determination of human thyroglobulin.serum thyroglobulin (tg) is a main marker of thyroid cancer relapses after total or near-total thyroidectomy of patients with differentiated thyroid carcinoma. in this study, we developed a chemiluminescent enzyme-linked immunosorbent assay (cl-elisa) for detecting tg in human serum. soybean peroxidase (sbp) in combination with 3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) and 4-morpholinopyridine (morph) and horseradish peroxidase (hrp) with p-iodophenol (pip) were used as detection systems ...201020851124
comparison of enzyme-linked immunosorbent assay-based techniques for the detection of antibody to rift valley fever virus in thermochemically inactivated sheep sera.different enzyme-linked immunosorbent assay (elisa)-based techniques for the detection of antibodies to rift valley fever virus (rvfv) have been developed in recent years, but their diagnostic sensitivity was not directly compared. in addition, their use might still be restricted to high biocontainment facilities when sera to be tested are collected from viremic individuals. in this study, we report on direct comparison of various elisa forms for the detection of anti-rvfv antibody in preinactiv ...201020854023
visualization of microvascular blood flow in mouse kidney and spleen by quantum dot injection with "in vivo cryotechnique".the "in vivo cryotechnique" (ivct) is a powerful tool to instantly capture blood flow, and all plasma components are well kept in tissue samples. in this study, we injected glutathione (gsh)-coated quantum dots (qds), which emit a 650-nm-fluorescent signal with an ultraviolet excitation, into anesthetized mouse left ventricles, and ivct was performed for kidneys, spleens and livers at 2, 5, 10, 15, 30s or 24h after the qd injection. the frozen tissues were processed to freeze-substitution fixati ...201020858507
a novel homogeneous biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum.electrochemiluminescence (ecl) assays have been widely used for the detection of anti-therapeutic antibodies (atas) against biotherapeutics. with the discontinuation of bioveris (bv) ecl platform, an alternative technology was needed to replace bv assays to ensure continuous support of multi-year clinical studies. after evaluation of several immunoassay platforms, a novel homogeneous biotin-digoxigenin (dig) based bridging elisa format was selected to develop an anti-rhumabx antibody screening a ...201020868690
angiotensin ii type 1 receptor (at1) signaling in astrocytes regulates synaptic degeneration-induced leukocyte entry to the central nervous system.astrocytes are the major cellular component of the blood-brain barrier glia limitans and act as regulators of leukocyte infiltration via chemokine expression. we have studied angiotensin-ii receptor type 1 (at1) and related nf-κb signaling in astrocytes. angiotensin ii derives from cleavage of angiotensin i by angiotensin converting enzyme (ace), angiotensin i deriving from angiotensinogen via cleavage by renin. level of expression of ace was slightly increased in transgenic mice that express do ...201020868737
ultrasensitive electrochemical immunoassay of staphylococcal enterotoxin b in food using enzyme-nanosilica-doped carbon nanotubes for signal amplification.a new sandwich-type electrochemical immunoassay for ultrasensitive detection of staphylococcal enterotoxin b (seb) in food was developed using horseradish peroxidase-nanosilica-doped multiwalled carbon nanotubes (hrpsicnts) for signal amplification. rabbit polyclonal anti-seb antibodies immobilized on the screen-printed carbon electrode (spce) and covalently bound to the hrpsicnts were used as capture antibodies and detection antibodies, respectively. in the presence of seb analyte, the sandwich ...201020873791
a peptide-based material platform for displaying antibodies to engage t cells.this study investigated a strategy by which antibodies are displayed to engage t cells. a peptide composite containing binding sites was characterized in vitro and explored as an injectable system in vivo. the composite consists of two amphiphilic peptides, aeaeakakaeaeakak (referred to as "eak") and eak appended with six consecutive histidines at the c-terminus ("eakh6"). spectroscopic analysis showed the two peptides integrated into a single structure. prior to combination, conformational anal ...201020880580
ternary surface monolayers for ultrasensitive (zeptomole) amperometric detection of nucleic acid hybridization without signal amplification.a ternary surface monolayer, consisting of coassembled thiolated capture probe, mercaptohexanol and dithiothreitol, is shown to offer dramatic improvements in the signal-to-noise characteristics of electrochemical dna hybridization biosensors based on common self-assembled monolayers. remarkably low detection limits down to 40 zmol (in 4 μl samples) as well as only 1 cfu escherichia coli per sensor are thus obtained without any additional amplification step in connection to the commonly used hor ...201020883023
potential of melatonin to treat or prevent age-related macular degeneration through stimulation of telomerase activity.melatonin may play a causal role in the occurrence of age-related macular degeneration (amd). replicative capacity and response to injury in the retinal pigment epithelium (rpe) is compromised during aging. prevention of telomere shortening by antioxidants may be a useful approach for reducing the cumulative effects of oxidative stress in rpe cells. melatonin, a well known antioxidant, which acts advantageously as an amphiphilic agent, may benefit amd patients more than commonly used lipophilic ...201020884126
increased plp1 gene expression leads to massive microglial cell activation and inflammation throughout the brain.pmd (pelizaeus-merzbacher disease) is a rare neurodegenerative disorder that impairs motor and cognitive functions and is associated with a shortened lifespan. the cause of pmd is mutations of the plp1 [proteolipid protein 1 gene (human)] gene. transgenic mice with increased plp1 [proteolipid protein 1 gene (non-human)] copy number model most aspects of pmd patients with duplications. hypomyelination and demyelination are believed to cause the neurological abnormalities in mammals with plp1 dupl ...201020885931
oxidative metabolism of the anti-cancer agent mitoxantrone by horseradish, lacto-and lignin peroxidase.mitoxantrone (mh(2)x), an anthraquinone-type anti-cancer agent used clinically in the treatment of human malignancies, is oxidatively activated by the peroxidase/h(2)o(2) enzyme system. in contrast to the enzymatic mechanisms of drug oxidation, the chemical transformations of mh(2)x are not well described. in this study, mh(2)x metabolites, produced by the horseradish, lacto- or lignin peroxidase (respectively hrp, lpo and lip)/h(2)o(2) system, were investigated by steady-state spectrokinetic an ...201020887767
one-chip biosensor for simultaneous disease marker/calibration substance measurement in human urine by electrochemical surface plasmon resonance method.we have developed a miniaturized electrochemical surface plasmon resonance biosensor for measuring two biomolecules that have very different molecular sizes, one is transferrin (mw=75 kda) as a disease marker protein, the other is creatinine (mw=113) as a calibration marker for the accurate measurement of human urinary samples. the sensor has a pdms based microchannel that is 2 mm wide and 20 μm deep. two gold films were integrated in the microchannel; one was modified with anti-transferrin anti ...201020888212
nanogold-actuated biomimetic peroxidase for sensitized electrochemical immunoassay of carcinoembryonic antigen in human serum.a new electrochemical immunoassay protocol for the determination of carcinoembryonic antigen (cea) in human serum was developed by means of immobilizing horseradish peroxidase-labeled anti-cea antibodies (hrp-anti-cea) on the nanogold/nano-fe(3)o(4) particles-functionalized polyion complex (pic) membrane. with a one-step immunoassay format, the formed immunocomplex inhibited partly the bioactive center of the immobilized hrp, and decreased the hrp toward the reduction of h(2)o(2). nanogold-actua ...201020888448
gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimer for sensitive electrochemical immunoassay of brevetoxins in food samples.a sensitive electrochemical immunosensor for the fast screening of brevetoxin b (btx-2) in food samples was developed by means of immobilizing btx-2-bovine serum albumin conjugate (btx-2-bsa) on the gold nanoparticles-decorated amine-terminated poly(amidoamine) dendrimers (aunp-paads). the presence of gold nanoparticles greatly improved the conductivity of the paads, and three-dimensional paads increased the surface coverage of the biomolecules on the electrode. under optimal conditions, three t ...201020926280
projections from the brain to the spinal cord in the mouse.the cells that project from the brain to the spinal cord have previously been mapped in a wide range of mammalian species, but have not been comprehensively studied in the mouse. we have mapped these cells in the mouse using retrograde tracing after large unilateral fluoro-gold (fg) and horseradish peroxidase (hrp) injections in the c1 and c2 spinal cord segments. we have identified over 30 cell groups that project to the spinal cord, and have confirmed that the pattern of major projections from ...201020936329
immunosensor based on fluorescence quenching matrix of the conducting polymer polypyrrole.in this study, the combination of autofluorescent proteins and fluorescence quenching polymers was shown to be a design which can increase the selectivity and sensitivity of immunosensors. with this objective, the conducting polymer polypyrrole (ppy) was used as a matrix for immobilization of proteins, which enables biological recognition of the analyte, and as a fluorescence quencher, which increases the selectivity of fluorescence-based detection. in this study, bovine leukemia virus proteins ...201020941482
simultaneous detection of multiple chemical residues in milk using broad-specificity antibodies in a hybrid immunosorbent assay.in this study, a novel immunoassay using 2 types of sensors (qds and an enzyme) were simultaneously used for detecting multiple structurally different molecules in milk. the method integrates the fluorescence-linked immunosorbent assay (flisa) using qd605 and qd655 as probes and an enzyme-linked immunosorbent assay (elisa) using horseradish peroxidase (hrp) labeled secondary antibody. the flisa was produced by anti-sulfonamide and anti-quinolone broad-specificity monoclonal antibodies (mabs) for ...201020947327
microfluidic immunosensor with gold nanoparticle platform for the determination of immunoglobulin g anti-echinococcus granulosus antibodies.this article describes a microfluidic immunosensor, developed for the detection of igg antibodies specific to echinococcus granulosus in human serum samples, which represents an alternative tool that can be used for the immunodiagnosis of hydatidosis in an automated way. our device consists of a plexiglas system with a central channel and a gold electrode. for immobilization of the e. granulosus antigen, a gold electrode was modified with the incorporation of gold nanoparticles. immobilized anti ...201020951112
a selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules.a new assay system for chitin has been developed. it comprises the chitin-binding protein chbb in fusion with a his-tag as well as with a strep-tag, the latter of which was chemically coupled to horseradish peroxidase. with the resulting complex, minimal quantities of chitin are photometrically detectable. in addition, the assay allows rapid scoring of the activity of chitin-synthases. as a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin bi ...201020957083
direct projections of omnipause neurons to reticulospinal neurons: a double-labeling light microscopic study in the cat.omnipause neurons (opns) are inhibitory neurons located in the midline region of the caudal pons. their role in gating the discharges of saccade-related burst neurons is well known, but there is no agreement concerning their influence on brainstem neurons that control other muscle groups participating in rapid gaze shifts. in the present study, we inquired whether opns project directly to pontobulbar reticulospinal neurons (rsns) in the cat. retrograde transport of horseradish peroxidase from th ...201020963829
ultrasensitive electrochemical aptasensor for thrombin based on the amplification of aptamer-aunps-hrp conjugates.successful development of an ultrasensitive and highly specific electrochemical aptasensor for thrombin based on amplification of aptamer-gold nanoparticles-horseradish peroxidase (aptamer-aunps-hrp) conjugates was reported. in this electrochemical protocol, aptamer1 (apt1) was immobilized on core/shell fe(3)o(4)/au magnetic nanoparticles (aumnps) and served as capture probe. aptamer2 (apt2) was dual labeled with aunps and hrp and used as detection probe. in the presence of thrombin, the sandwic ...201021030239
nutritive evaluation and effect of moringa oleifera pod on clastogenic potential in the mouse.moringa oleifera lam (horseradish tree; tender pod or fruits) has been consumed as a vegetable and utilized as a major ingredient of healthy thai cuisine. previous studies have shown that m. oleifera pod extracts act as bifunctional inducers along with displaying antioxidant properties and also inhibiting skin papillomagenesis in mice. this study was aimed to determine the nutritive value, and clastogenic and anticlastogenic potentials of m. oleifera pod. the nutritive value was determined accor ...201021039028
peripheral nerve injury and trpv1-expressing primary afferent c-fibers cause opening of the blood-brain barrier.the blood-brain barrier (bbb) plays the crucial role of limiting exposure of the central nervous system (cns) to damaging molecules and cells. dysfunction of the bbb is critical in a broad range of cns disorders including neurodegeneration, inflammatory or traumatic injury to the cns, and stroke. in peripheral tissues, the vascular-tissue permeability is normally greater than bbb permeability, but vascular leakage can be induced by efferent discharge activity in primary sensory neurons leading t ...201021044346
prognostic significance of cyclin a and b1 in pediatric embryonal tumors.embryonal tumors constitute the most common malignant brain tumor group in children. although patient prognosis has been substantially improved over recent decades, identification of prognostic markers would be of obvious significance. in the present study we evaluated the prognostic significance of cyclin a and b1 in correlation with ki-67 index in pediatric embryonal tumors. we retrospectively evaluated 53 children with embryonic tumors who were treated surgically in our institute. all patient ...201021069428
increasing of blood-tumor barrier permeability through paracellular pathway by low-frequency ultrasound irradiation in vitro.the research was conducted to study the increase of blood-tumor barrier (btb) permeability through paracellular pathway by low-frequency ultrasound (lfu) irradiation in vitro. lfu (frequency=1.0 mhz) was performed to irradiate btb model from the co-culture of rat c6 glioma cells and rat brain microvascular endothelial cells (rbmecs). the permeability of btb was measured by transendothelial electrical resistance (teer) and flux of horseradish peroxidase (hrp) assays after lfu irradiation. western ...201021104456
stopped-flow microarray immunoassay for detection of viable e. coli by use of chemiluminescence flow-through microarrays.rapid multiplexed analysis of microorganisms is important in water analysis to control bacterial contamination for health and safety reasons. direct quantification of bacteria by means of flow-through microarray immunoassays requires new analysis strategies for optimising sensitivity and the analysis time. for bacteria and for particles, hydrodynamic forces and sedimentation are the dominating effects for binding on surfaces in a flow-through system, whereas diffusion is insignificant. therefore ...201021107820
kinetic analysis of phagosomal production of reactive oxygen species.phagocytes produce large quantities of reactive oxygen species for pathogen killing; however, the kinetics and amplitude of ros production on the level of individual phagosomes are poorly understood. this is mainly due to the lack of appropriate methods for quantitative ros detection with microscopic resolution. we covalently attached the ros-sensitive dye dichlorodihydrofluorescein (dcfh(2)) to yeast particles and investigated their fluorescence due to oxidation in vitro and in live phagocytes. ...201021111807
enzyme linked immunosorbent assay for milk progesterone.a direct antigen heterologous enzyme linked immunosorbent assay (elisa) for milk progesterone has been developed using progesterone-3-o-carboxymethyloxime-bovine serum albumin (p-3-o-cmo-bsa) antiserum and 17-α-hydroxy-progesterone-3-o-carboxymethyloxime-horseradish peroxidase (17-α-oh-p-3-o-cmo-hrp) enzyme conjugate. the data of the present study reveal that the homologous assay, which employed p-3-o-cmo-hrp as the label, showed no displacement. on the contrary, replacement of p-3-o-cmo-hrp wit ...201021113843
cation exchange in znse nanocrystals for signal amplification in bioassays.znse nanocrystals (ncs), possessing low native luminescence but high biocompatibility, were employed as labeling tags in bioassays. they were able to amplify each target recognition event thousands of times through a cation-exchange reaction (cxamp) that released over 3000 encapsulated zn(2+) from one single nc. the freed cations in turn triggered strong fluorescence from the zn-responsive dyes. the present study demonstrated that cxamp with znse delivered superior detection performance in compa ...201021117624
quantitative detection of human tumor necrosis factor α by a resonance raman enzyme-linked immunosorbent assay.tumor necrosis factor α is an inflammatory cytokine which has been linked with many infectious and inflammatory diseases. detection and quantification of this key biomarker is commonly achieved by use of an enzyme-linked immunosorbent assay (elisa). this fundamental technique uses the spectroscopic detection of a chromogen such as 3,3',5,5'-tetramethylbenzidine (tmb). horseradish peroxidase (hrp), bound to the detection antibody, catalyzes the oxidation of tmb by hydrogen peroxide to generate co ...201021121688
the expression of nadph oxidases and production of reactive oxygen species by human lung adenocarcinoma epithelial cell line a549.controlled production of reactive oxygen species (ros) by nadph oxidases in non-phagocytic cells has recently been suggested to participate in the regulation of cellular functions. due to the role of ros in control of cellular functions, precise and accurate detection of ros is of essential importance. however, various methodological approaches currently used for ros determination vary in sensitivity, specificity, as well as in requirements for specialized equipment. in this study, human lung ep ...201021138653
site-specific protein cross-linking by peroxidase-catalyzed activation of a tyrosine-containing peptide tag.protein modification methods represent fundamental techniques that are applicable in many fields. in this study, a site-specific protein cross-linking based on the oxidative tyrosine coupling reaction was demonstrated. in the presence of horseradish peroxidase (hrp) and h(2)o(2), tyrosine residues undergo one-electron oxidation reactions and form radicals in their phenolic moieties, and these species subsequently react with each other to form dimers or further react to generate polymers. here, a ...201021142129
miniaturised hybrid immunoassay for high sensitivity analysis of aflatoxin m1 in milk.an ultra-sensitive sandwich elisa was developed for detection of afm1 in milk. the assay involved the immobilization of rat monoclonal antibody of afm1 in 384 microtiter plate to capture afm1 antigen. this was detected by tracer secondary rabbit poly-clonal antibody labelled with horseradish peroxidase upon addition of a luminol-based substrate. milk samples with different fat percentage were analyzed after pre-treatment. linear range of afm1 detection 250-6.25 pg/ml was achieved in 3% fat milk. ...201021145226
lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle.lactobacilli have been barely studied in cows. we proposed isolate and characterize lactic acid bacteria from dairy cows as compared to those raised for meat production and elucidate the presence of strains with evident probiotic employment's potential. for this, isolation and quantification of lab mainly lactobacilli were realized from vaginal cattle samples in mrs medium. each selected microorganism was then briefly characterized. the math method was employed using hexadecane, xilene an toluen ...201021145403
citrinin (cit) determination in rice samples using a micro fluidic electrochemical immunosensor.the development of an electrochemical immunosensor incorporated in a micro fluidic cell for quantification of citrinin (cit) mycotoxin in rice samples is described for the first time. both cit present in rice samples and immobilized on a gold surface electrodeposited on a glassy carbon (gc) electrode modified with a cysteamine self-assembled monolayer were allowed to compete for the monoclonal mouse anti-cit igg antibody (mab-cit) present in solution. then, an excess of rabbit anti mouse igg (h+ ...201021147345
overexpression of androgen receptors in target musculature confers androgen sensitivity to motoneuron dendrites.the dendritic arbors of spinal motoneurons are dynamically regulated by a variety of factors, and several lines of evidence indicate that trophic interactions with the target musculature are of central importance. in highly androgen-sensitive motoneuron populations, androgens are thought to regulate motoneuron dendrites through their action at the receptor-enriched target musculature. using rats transgenically modified to overexpress androgen receptor (ar) in skeletal muscle, we directly tested ...201021147875
high sensitivity dna detection using gold nanoparticle functionalised polyaniline nanofibres.polyaniline (pani) nanofibres (pani-nf) have been modified with chemically grown gold nanoparticles to give a nanocomposite material (pani-nf-aunp) and deposited on gold electrodes. single stranded capture dna was then bound to the gold nanoparticles and the underlying gold electrode and allowed to hybridise with a complementary target strand that is uniquely associated with the pathogen, staphylococcus aureus (s. aureus), that causes mastitis. significantly, cyclic voltammetry demonstrates that ...201021159503
ultrastructural analysis of glutamate-immunopositive synapses onto the rat jaw-closing motoneurons during postnatal development.the excitatory synapses on the jaw-closing (jc) motoneurons mediate the neuronal input that ensures smooth and rhythmic movements of the jaw. recently, we have shown that the neurotransmitter phenotype of the inhibitory boutons onto jc motoneurons shifts from gaba to glycine, and new inhibitory synapses onto jc motoneurons are continuously formed during postnatal development (paik et al. [2007] j. comp. neurol. 503:779–789). to test whether the developmental pattern of the excitatory synapses on ...201021162123
polyethylene glycol enhances axolemmal resealing following transection in cultured cells and in ex vivo spinal cord.the integrity of the neuronal membrane is critical for its function as well as survival, and ineffective repair of damaged membranes may be one of the key factors underlying the neuronal degeneration and overall functional loss that occurs after spinal cord injury and traumatic brain injury. previously, we showed that polyethylene glycol (peg) can reseal axonal membranes following compression in isolated guinea pig spinal cord white matter. we now report that 10 mm peg can also significantly enh ...201019691421
analysis of peroxidase activity of rice (oryza sativa) recombinant hemoglobin 1: implications for in vivo function of hexacoordinate non-symbiotic hemoglobins in plants.in plants, it has been proposed that hexacoordinate (class 1) non-symbiotic hbs (nshb-1) function in vivo as peroxidases. however, little is known about peroxidase activity of nshb-1. we evaluated the peroxidase activity of rice recombinant hb1 (a nshb-1) by using the guaiacol/h2o2 system at ph 6.0 and compared it to that from horseradish peroxidase (hrp). results showed that the affinity of rice hb1 for h2o2 was 86-times lower than that of hrp (k(m)=23.3 and 0.27 mm, respectively) and that the ...201019833360
factors in creepy delayed neuronal death in hippocampus following brain ischemia-reperfusion injury with long-term survival.brain ischemia is well known for its ability to compromise the function of the blood-brain barrier. we assessed blood-brain barrier integrity by examining the leakage of horseradish peroxidase (hrp) and different fragments of amyloid precursor protein from the vascular network into hippocampal parenchyma in rats exposed to brain ischemia with long-term survival. the areas of blood-brain barrier leakage were associated with increased staining of hrp and c-terminal of amyloid precursor protein/bet ...201019812917
bionanocapsule-based enzyme-antibody conjugates for enzyme-linked immunosorbent assay.macromolecules that can assemble a large number of enzyme and antibody molecules have been used frequently for improvement of sensitivities in enzyme-linked immunosorbent assays (elisas). we generated bionanocapsules (bncs) of approximately 30nm displaying immunoglobulin g (igg) fc-binding zz domains derived from staphylococcus aureus protein a (designated as zz-bnc). in the conventional elisa using primary antibody and horseradish peroxidase-labeled secondary antibody for detecting antigen on t ...201019819216
electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor.polypyrrole (ppy) has been shown as a matrix for label-free electrochemical immunosensor based on electrochemical impedance spectroscopy (eis) measurements. the immunosensing system model presented here was based on bovine leukemia virus (blv) protein (gp51) entrapped within electrochemically-synthesized polypyrrole (ppy/gp51). this ppy/gp51 layer interacted with antibodies against gp51 (anti-gp51-ab) that are present in significant concentration in the blood serum of blv infected cattle. after ...201019879816
e. coli outer membrane with autodisplayed z-domain as a molecular recognition layer of spr biosensor.in this work, the z-domain of protein a was expressed as a recombinant-fusion protein on the outer membrane of escherichia coli by using "autodisplay" technology, and the outer membrane with z-domains was layered on a spr biosensor as a highly sensitive molecular recognition layer. the outer membrane layer formation was demonstrated by using various surfaces with controlled hydrophobicity. the effect of orientation control by the outer membrane with z-domains was estimated to have far higher sen ...201019884000
highly porous bioresorbable scaffolds with controlled release of bioactive agents for tissue-regeneration applications.highly porous poly(dl-lactic-co-glycolic acid) films with controlled release of horseradish peroxidase (hrp) as a model protein have been successfully developed and studied. these films, which are prepared by freeze-drying inverted emulsions, are designed for use in tissue-regeneration applications. the effects of the emulsion's formulation and host polymer's characteristics on the film's microstructure and hrp release profile over 4 weeks were investigated. a dual pore size population is charac ...201019887123
injectable biodegradable hydrogels with tunable mechanical properties for the stimulation of neurogenesic differentiation of human mesenchymal stem cells in 3d culture.we report an injectable hydrogel scaffold system with tunable stiffness for controlling the proliferation rate and differentiation of human mesenchymal stem cells (hmscs) in a three-dimensional (3d) context in normal growth media. the hydrogels composed of gelatin-hydroxyphenylpropionic acid (gtn-hpa) conjugate were formed using the oxidative coupling of hpa moieties catalyzed by hydrogen peroxide (h(2)o(2)) and horseradish peroxidase (hrp). the stiffness of the hydrogels was readily tuned by va ...201019892395
enhanced detection of staphylococcal genomes in positive blood cultures using a polymeric enzyme complex.this article describes a simple and inexpensive signal amplification method, termed polymeric enzyme detection (ped), which permits rapid and sensitive detection of conserved sequences in the tuf gene that identify staphylococcus genus, conserved sequences in the femb gene that specifically detect staphylococcus aureus species, and the methicillin resistance gene meca directly from positive blood culture bottles. microbe-specific capture probes were immobilized onto microtiter plates or silicon ...201019782653
comparison of gut microbiota and allergic reactions in balb/c mice fed different cultivars of rice.our preliminary clinical trial showed that consumption of cooked rice of a japanese common cultivar yukihikari improved atopic dermatitis associated with a suspected rice allergy, although the underlying mechanisms remain unclear. we hypothesised that the ameliorating effect of yukihikari on atopic dermatitis is associated with the gut microbiota. balb/c mice were fed a synthetic diet supplemented with uncooked and polished white rice powder prepared from one of four different cultivars: yukihik ...201019772680
direct synaptic connections between superior colliculus afferents and thalamo-insular projection neurons in the feline suprageniculate nucleus: a double-labeling study with wga-hrp and kainic acid.the suprageniculate nucleus (sg) of the feline thalamus, which subserves largely unimodal sensory and orientation behavior, receives input from the deep layers of the superior colliculus (sc), and projects to the suprasylvian cortical areas, such as the anterior ectosylvian visual area and the insular visual area (iva), which contain visually responsive neurons. through a double tract-tracing procedure involving the injection of wheat germ agglutinin conjugated with horseradish peroxidase (wga-h ...201019755134
microbicidal action of indole-3-acetic acid combined with horseradish peroxidase on prototheca zopfii from bovine mastitis.we investigated the toxic effect of indole-3-acetic acid (iaa) combined with horseradish peroxidase (hrp) on prototheca zopfii from bovine mastitis. p. zopfii isolates were identified and characterized by morpho-physiological parameters; presences of p. zopfii genotype 2 were also investigated. subsequently, p. zopfii was incubated in the absence (control) or presence of iaa/hrp and examined for: (i) cell viability; (ii) colonies number formation; (iii) antioxidant enzyme activity; and (iv) dna ...201019707886
transtactin: a universal transmembrane delivery system for strep-tag ii-fused cargos.the delivery of molecules into cells poses a critical problem that has to be solved for the development of diagnostic tools and therapeutic agents acting on intracellular targets. cargos which by themselves cannot penetrate cellular membranes due to their biophysical properties can achieve cell membrane permeability by fusion to protein transduction domains (ptds). here, we engineered a universal delivery system based on ptd-fused strep-tactin, which we named transtactin. biochemical characteriz ...201019602053
decontamination of chemical and biological warfare agents with a single multi-functional material.we report the synthesis of new polymers based on a dimethylacrylamide-methacrylate (dmaa-ma) co-polymer backbone that support both chemical and biological agent decontamination. polyurethanes containing the redox enzymes glucose oxidase and horseradish peroxidase can convert halide ions into active halogens and exert striking bactericidal activity against gram positive and gram negative bacteria. new materials combining those biopolymers with a family of n-alkyl 4-pyridinium aldoxime (4-pam) hal ...201020199807
synapse formation in the olfactory cortex by regenerating optic axons: ultrastructural evidence for polyspecific chemoaffinity.the optic nerve was severed at its entry into the optic chiasma and implanted into the striatal region of the ipsilateral cerebral hemisphere in adult frogs (rana pipiens). regenerating retinal ganglion cell axons, observed by the autoradiographic tracing method and by horseradish peroxidase (hrp) fiber filling, grew anteriorly along the olfactory tracts and posteriorly along the ipsilateral lateral forebrain bundle and stria medullaris. many of the regenerating axons ultimately joined the ipsil ...20102822778
oligonucleotide microarray for the identification of carbapenemase genes of molecular classes a, b, and d.this work is a report on the development of a method of hybridization analysis on dna microarrays for the simultaneous identification and typing of carbapenemase-encoding genes. these enzymes are produced by the microorganisms that are responsible for causing infectious diseases. the method involves several steps, including dna extraction from clinical samples and amplification of carbapenemase genes by multiplex pcr with simultaneous labelling by biotin. following that, hybridization of the lab ...201022649657
in situ generation of hydrogen peroxide by carbohydrate oxidase and cellobiose dehydrogenase for bleaching purposes.the carbohydrate oxidase from microdochium nivale (caox), heterologously expressed in aspergillus oryzae, and cellobiose dehydrogenase from myriococcum thermophilum (mtcdh), were assessed for their ability to generate bleaching species at a ph suitable for liquid detergents. the substrate specificities of caox and mtcdh were analyzed on a large variety of soluble and insoluble substrates, using oxygen as an electron receptor. even insoluble substrates like cellulose were oxidized from both caox ...201021298807
amperometric hydrogen peroxide biosensor based on the immobilization of heme proteins on gold nanoparticles-bacteria cellulose nanofibers nanocomposite.a novel matrix, gold nanoparticles-bacterial cellulose nanofibers (au-bc) nanocomposite was developed for enzyme immobilization and biosensor fabrication due to its unique properties such as satisfying biocompatibility, good conductivity and extensive surface area, which were inherited from both gold nanoparticles (aunps) and bacterial cellulose nanofibers (bc). heme proteins such as horseradish peroxidase (hrp), hemoglobin (hb) and myoglobin (mb) were successfully immobilized on the surface of ...201021315900
metabolic stability of superoxide and hydroxyl radical adducts of a cyclic nitrone toward rat liver microsomes and cytosol: a stopped-flow esr spectroscopy study.the metabolic stability of the spin adducts derived from the reaction of superoxide and hydroxyl radicals with 5-tert-butoxycarbonyl-5-methyl-1-pyrroline n-oxide (bocmpo) in the presence of rat liver microsomes (rlm) and rat liver cytosol (rlc) was studied by using a stopped-flow device coupled to an electron spin resonance (esr) spectrometer. the kinetics of the disappearance of the bocmpo-oh and bocmpo-ooh radicals could be followed by esr spectroscopy with treatment of the esr data by an appr ...201020452418
protection against early intestinal compromise by lipid-rich enteral nutrition through cholecystokinin receptors.early gut wall integrity loss and local intestinal inflammation are associated with the development of inflammatory complications in surgical and trauma patients. prevention of these intestinal events is a potential target for therapies aimed to control systemic inflammation. previously, we demonstrated in a rodent shock model that lipid-rich enteral nutrition attenuated systemic inflammation and prevented organ damage through a cholecystokinin receptor-dependent vagal pathway. the influence of ...201020453642
delivering horseradish peroxidase as a respirable powder to the isolated, perfused, and ventilated lung of the rat: the pulmonary disposition of an inhaled model biopharmaceutical.our aim was to investigate the potential of the dustgun aerosol technology integrated with the isolated, perfused, and ventilated lung of the rat (ipl) to study the pulmonary disposition of an inhaled model biopharmaceutical, the 40-kda protein horseradish peroxidase (hrp).201020455769
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